IleRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following IleRS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IleRS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa19418 NM_176387.2
Latest version!
Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa19418 NM_080101.4
Latest version!
Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa19418 NM_168952.4
Latest version!
Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa19418
    Clone ID Related Accession (Same CDS sequence) NM_168952.4 , NM_080101.4 , NM_176387.2
    Accession Version NM_168952.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Isoleucyl-tRNA synthetase, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168952.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
    GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC
    CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG
    CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA
    TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG
    GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA
    ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG
    AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC
    CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC
    TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC
    GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC
    GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA
    AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC
    GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG
    TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT
    TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG
    TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC
    GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA
    GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA
    GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC
    CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG
    GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC
    CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG
    GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG
    TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC
    GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA
    GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA
    GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC
    AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA
    CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT
    GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT
    CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG
    CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA
    GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT
    GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG
    AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC
    GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA
    AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT
    GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA
    ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG
    GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG
    CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA
    TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT
    GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG
    CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG
    AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG
    GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG
    CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC
    ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG
    GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT
    GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC
    GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT
    CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG
    TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG
    CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG
    CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA
    AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG
    CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT
    TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC
    GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA
    CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG
    GAAAACCCCT TGGGACGCGC CTTATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_730716.1
    CDS133..3822
    Translation

    Target ORF information:

    RefSeq Version NM_168952.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168952.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
    GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC
    CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG
    CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA
    TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG
    GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA
    ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG
    AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC
    CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC
    TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC
    GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC
    GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA
    AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC
    GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG
    TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT
    TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG
    TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC
    GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA
    GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA
    GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC
    CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG
    GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC
    CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG
    GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG
    TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC
    GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA
    GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA
    GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC
    AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA
    CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT
    GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT
    CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG
    CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA
    GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT
    GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG
    AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC
    GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA
    AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT
    GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA
    ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG
    GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG
    CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA
    TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT
    GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG
    CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG
    AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG
    GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG
    CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC
    ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG
    GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT
    GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC
    GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT
    CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG
    TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG
    CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG
    CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA
    AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG
    CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT
    TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC
    GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA
    CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG
    GAAAACCCCT TGGGACGCGC CTTATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19418
    Clone ID Related Accession (Same CDS sequence) NM_168952.4 , NM_080101.4 , NM_176387.2
    Accession Version NM_080101.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Isoleucyl-tRNA synthetase, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080101.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
    GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC
    CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG
    CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA
    TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG
    GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA
    ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG
    AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC
    CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC
    TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC
    GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC
    GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA
    AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC
    GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG
    TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT
    TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG
    TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC
    GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA
    GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA
    GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC
    CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG
    GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC
    CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG
    GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG
    TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC
    GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA
    GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA
    GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC
    AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA
    CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT
    GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT
    CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG
    CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA
    GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT
    GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG
    AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC
    GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA
    AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT
    GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA
    ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG
    GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG
    CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA
    TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT
    GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG
    CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG
    AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG
    GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG
    CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC
    ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG
    GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT
    GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC
    GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT
    CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG
    TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG
    CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG
    CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA
    AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG
    CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT
    TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC
    GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA
    CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG
    GAAAACCCCT TGGGACGCGC CTTATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_524840.2
    CDS54..3743
    Translation

    Target ORF information:

    RefSeq Version NM_080101.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_080101.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
    GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC
    CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG
    CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA
    TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG
    GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA
    ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG
    AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC
    CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC
    TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC
    GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC
    GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA
    AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC
    GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG
    TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT
    TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG
    TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC
    GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA
    GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA
    GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC
    CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG
    GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC
    CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG
    GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG
    TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC
    GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA
    GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA
    GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC
    AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA
    CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT
    GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT
    CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG
    CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA
    GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT
    GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG
    AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC
    GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA
    AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT
    GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA
    ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG
    GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG
    CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA
    TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT
    GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG
    CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG
    AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG
    GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG
    CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC
    ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG
    GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT
    GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC
    GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT
    CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG
    TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG
    CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG
    CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA
    AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG
    CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT
    TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC
    GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA
    CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG
    GAAAACCCCT TGGGACGCGC CTTATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19418
    Clone ID Related Accession (Same CDS sequence) NM_168952.4 , NM_080101.4 , NM_176387.2
    Accession Version NM_176387.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Isoleucyl-tRNA synthetase, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_176387.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
    GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC
    CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG
    CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA
    TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG
    GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA
    ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG
    AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC
    CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC
    TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC
    GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC
    GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA
    AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC
    GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG
    TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT
    TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG
    TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC
    GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA
    GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA
    GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC
    CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG
    GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC
    CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG
    GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG
    TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC
    GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA
    GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA
    GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC
    AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA
    CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT
    GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT
    CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG
    CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA
    GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT
    GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG
    AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC
    GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA
    AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT
    GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA
    ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG
    GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG
    CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA
    TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT
    GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG
    CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG
    AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG
    GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG
    CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC
    ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG
    GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT
    GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC
    GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT
    CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG
    TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG
    CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG
    CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA
    AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG
    CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT
    TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC
    GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA
    CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG
    GAAAACCCCT TGGGACGCGC CTTATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788565.1
    CDS79..3768
    Translation

    Target ORF information:

    RefSeq Version NM_176387.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176387.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
    GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC
    CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG
    CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA
    TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG
    GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA
    ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG
    AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC
    CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC
    TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC
    GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC
    GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA
    AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC
    GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG
    TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT
    TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG
    TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC
    GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA
    GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA
    GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC
    CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG
    GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC
    CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG
    GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG
    TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC
    GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA
    GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA
    GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC
    AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA
    CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT
    GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT
    CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG
    CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA
    GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT
    GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG
    AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC
    GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA
    AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT
    GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA
    ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG
    GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG
    CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA
    TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT
    GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG
    CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG
    AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG
    GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG
    CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC
    ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG
    GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT
    GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC
    GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT
    CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG
    TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG
    CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG
    CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA
    AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG
    CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT
    TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC
    GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA
    CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG
    GAAAACCCCT TGGGACGCGC CTTATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.