spin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spin
Entrez Gene ID	45380
Full Name	spinster
Synonyms	25/11,CG8428,Dmel\CG8428,Spin,bnch,l(2)10403,l(2)W5,l(2)k02511,l(2)k09905,l(2R)W5
General protein information	Preferred Names spinster Names CG8428 gene product from transcript CG8428-RB CG8428-PA CG8428-PB CG8428-PC CG8428-PD CG8428-PE Spinster/Benchwarmer benchwarmer diphthong lethal (2) W5 spin-PA spin-PB spin-PC spin-PD spin-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52F5-52E

mRNA and Protein(s)

mRNA	Protein	Name
NM_166145.2	NP_725531.1	spinster, isoform A
NM_080084.3	NP_524823.1	spinster, isoform D
NM_166147.2	NP_725533.1	spinster, isoform E
NM_166144.2	NP_725530.1	spinster, isoform C
NM_166146.2	NP_725532.1	spinster, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPNS1	XP_003639185.2
Bos taurus (cattle)	SPNS1	NP_001070276.1
Drosophila melanogaster (fruit fly)	spin	NP_725530.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006346	XP_316366.4
Caenorhabditis elegans (roundworm)	F09A5.1	NP_510181.3
Mus musculus (house mouse)	Spns1	NP_076201.2
Danio rerio (zebrafish)	spns1	NP_705949.1
	SPNS1	XP_001102328.2
Caenorhabditis elegans (roundworm)	CELE_C39E9.10	NP_502513.1
Arabidopsis thaliana (thale cress)	AT5G65687	NP_680469.1
Arabidopsis thaliana (thale cress)	AT2G22730	NP_179858.2
Arabidopsis thaliana (thale cress)	AT5G64500	NP_201255.1
Xenopus tropicalis (tropical clawed frog)	spns1	NP_001106495.1
Homo sapiens (human)	SPNS1	NP_114427.1
Pan troglodytes (chimpanzee)	SPNS1	XP_001147131.2
Caenorhabditis elegans (roundworm)	C13C4.5	NP_506041.1
Rattus norvegicus (Norway rat)	Spns1	NP_001034297.2
Caenorhabditis elegans (roundworm)	CELE_Y111B2A.19	NP_499650.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Within the migrating perineurial glial cells, Spinster is required to downregulate Decapentaplegic signaling activity, which ceases migratory abilities. Also, Spinster affects the growth of the carpet cell, which indirectly modulates glial migration.
Title: Spinster controls Dpp signaling during glial migration in the Drosophila eye.

Spinster (Spin) encodes a putative lysosomal efflux permease with the hallmarks of a sugar transporter. Spin mutants accumulate lysosomal carbohydrates and enlarged lysosomes.
Title: Spinster is required for autophagic lysosome reformation and mTOR reactivation following starvation.

spin promotes cell death mechanisms that are regulated negatively by th and emc and positively by atg8a
Title: Phenotypic interactions of spinster with the genes encoding proteins for cell death control in Drosophila melanogaster.

Spin is expressed in nerve and muscle and is required for normal synaptic growth. Spin is found in a late endosomal compartment;there is evidence for altered endosomal/lysosomal function in Spin-deficient flies.
Title: Unrestricted synaptic growth in spinster-a late endosomal protein implicated in TGF-beta-mediated synaptic growth regulation.

Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis.
Title: Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis.

The following spin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11403	NM_166146.2 Latest version!	Drosophila melanogaster spinster (spin), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11404	NM_166144.2 Latest version!	Drosophila melanogaster spinster (spin), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11405	NM_080084.3 Latest version!	Drosophila melanogaster spinster (spin), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11406	NM_166145.2 Latest version!	Drosophila melanogaster spinster (spin), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11407	NM_166147.2 Latest version!	Drosophila melanogaster spinster (spin), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spin ( NM_166146.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725532.1 Drosophila melanogaster spinster (spin), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11403
Clone ID Related Accession (Same CDS sequence)	NM_166146.2
Accession Version	NM_166146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spinster, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166146.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTGA AACACCAGAA GCAATCCTAC CAACCGCTCC CAACGGCGGC CGCCATGGAC 
AATCCGGCGA TGATCCAGAG CAGCGGTAGC AGTGGGAGCA GCTCCTCTGA GGAGGGCGGG 
AGTCGTGAGG ATGTGGCCAA TCTATCGCCG CTCGGACTGC CTACGACCTA CTCGTCACAG 
CAATTGATGC CCTCGGATAC GGACAGCATG GAGGAGGAGC GGCACCGACT GCGTCCCCAC 
CACCATCACC ACCACCCACT GGGCGAGCAC CACCACATCC CGGGCATACC ACCATCCGCC 
GTCGTGCCAT CGCGTCTCTC CAGCGTGGGC AGATCGCAGT GGTTCACCGT TACCGTGCTA 
TGCTTCGTCA ACCTCATCAA CTACATGGAT CGCTTTACGA TCGCAGGAGT TCTCACAGAT 
GTCCGAAATG ATTTCGATAT TGGCAATGAT AGTGCCGGAC TTCTCCAGAC GGTCTTCGTT 
ATCTCGTACA TGGTATGCGC CCCCATCTTC GGCTATCTGG GCGATCGTTA CTCCCGCCCC 
TGGATAATGG CGGTGGGCGT GGGTCTGTGG AGCACCACCA CCCTACTGGG GTCGTTCATG 
AAGCAGTTTG GATGGTTCAT CGCCTTCAGA GCGCTGGTCG GGATCGGCGA GGCATCGTAC 
AGTACCATCG CACCGACGAT TATCTCGGAT CTGTTCGTGC ACGACATGCG CTCCAAGATG 
CTGGCACTGT TCTACTTTGC CATACCCGTG GGATCCGGTC TGGGATACAT TGTGGGATCC 
AAGACAGCGC ACCTGGCCAA CGATTGGCGC TGGGCTCTGA GGGTCACGCC CATTCTGGGC 
ATTGTGGCAG TTTTCCTCAT TTTGCTGATC AAGGATCCGG TGCGGGGTCA CAGCGAGGGA 
TCGCACAATT TGGAGGCCAC TACCTACAAG CAGGACATCA AGGCGTTGGT CAGGAACCGT 
TCCTTCATGC TCTCCACGGC TGGATTCACG TGCGTTGCCT TCGTGGCCGG AGCACTGGCC 
TGGTGGGGAC CTTCCTTCAT CTATCTAGGC ATGAAGATGC AGCCCGGCAA CGAGAACATT 
GTCCAGGACG ACATCTCGTA CAAGTTTGGT CTGGTGGCCA TGTTGGCCGG ACTCATCGGA 
GTCCCGTTGG GCTCCTTTCT GGCCCAGCGA TTGAGGGGTC GCTACGAGAA CTGTGATCCG 
TACATCTGTG CGGTGGGACT TTTCATCTCG GCTCCGATGG TCTTCGCTGC TCTCGTGGTG 
CCGCAGACAA GCGAGAGCCT CTGCTTTTTC TTCGTTTTCG TGGCCCAGGT GGCACTGAAC 
CTGTGCTGGT CCATTGTAGC CGACATCCTA CTGTATGTGG TGGTTCCCAC GCGACGTTCA 
ACAGCCGAGG CCTTCCAAAT CCTCATCTCA CACGCACTCG GTGATGCCGG CAGTCCGTAT 
TTGGTTGGAG CAATCTCCGA GGCCATCATG AAACACCTGC ACAAGAACCC CAGCGACTCT 
GGACTGACCA CCGAGCTGCG AAGCATGTCC CAAGTGGCAG GTTCCGCGAT CAGTAATGCC 
ACCCAGGTGA TCGCGGAAGC CACCACCAGT TTGATGGAAA CAGCACGCTC GTCAGCCTCT 
CAGGAATACT CTGATGTGGA GCAATTCGAG GGACTGCAAT ATGCGCTCTT CTCCACCAGT 
TTTGTGGAGG TGCTGGGCGG CATTTTCTTC ATATTCACCG CCTGCTTTAT CATCAAAGAC 
AAGTACAATG CCACACGAGG ACTGCAAGGC GATCAGGGAG CGCAGGCGGT TCGATCGTCA 
GTCGCCTTGG CCAGCGGGCA AAAGGACGTC GAGTCCTTCA ACTCGGACTG CCTGGTCCTT 
TGCACTGACA TCGCGCTGCG CGAACGCACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725532.1
CDS	397..2289
Translation	MSLKHQKQSYQPLPTAAAMDNPAMIQSSGSSGSSSSEEGGSREDVANLSPLGLPTTYSSQQLMPSDTDSMEEERHRLRPHHHHHHPLGEHHHIPGIPPSAVVPSRLSSVGRSQWFTVTVLCFVNLINYMDRFTIAGVLTDVRNDFDIGNDSAGLLQTVFVISYMVCAPIFGYLGDRYSRPWIMAVGVGLWSTTTLLGSFMKQFGWFIAFRALVGIGEASYSTIAPTIISDLFVHDMRSKMLALFYFAIPVGSGLGYIVGSKTAHLANDWRWALRVTPILGIVAVFLILLIKDPVRGHSEGSHNLEATTYKQDIKALVRNRSFMLSTAGFTCVAFVAGALAWWGPSFIYLGMKMQPGNENIVQDDISYKFGLVAMLAGLIGVPLGSFLAQRLRGRYENCDPYICAVGLFISAPMVFAALVVPQTSESLCFFFVFVAQVALNLCWSIVADILLYVVVPTRRSTAEAFQILISHALGDAGSPYLVGAISEAIMKHLHKNPSDSGLTTELRSMSQVAGSAISNATQVIAEATTSLMETARSSASQEYSDVEQFEGLQYALFSTSFVEVLGGIFFIFTACFIIKDKYNATRGLQGDQGAQAVRSSVALASGQKDVESFNSDCLVLCTDIALRERT

Target ORF information:

RefSeq Version	NM_166146.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spinster (spin), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166146.2

ORF Insert Sequence:

ATGTCGCTGA AACACCAGAA GCAATCCTAC CAACCGCTCC CAACGGCGGC CGCCATGGAC 
AATCCGGCGA TGATCCAGAG CAGCGGTAGC AGTGGGAGCA GCTCCTCTGA GGAGGGCGGG 
AGTCGTGAGG ATGTGGCCAA TCTATCGCCG CTCGGACTGC CTACGACCTA CTCGTCACAG 
CAATTGATGC CCTCGGATAC GGACAGCATG GAGGAGGAGC GGCACCGACT GCGTCCCCAC 
CACCATCACC ACCACCCACT GGGCGAGCAC CACCACATCC CGGGCATACC ACCATCCGCC 
GTCGTGCCAT CGCGTCTCTC CAGCGTGGGC AGATCGCAGT GGTTCACCGT TACCGTGCTA 
TGCTTCGTCA ACCTCATCAA CTACATGGAT CGCTTTACGA TCGCAGGAGT TCTCACAGAT 
GTCCGAAATG ATTTCGATAT TGGCAATGAT AGTGCCGGAC TTCTCCAGAC GGTCTTCGTT 
ATCTCGTACA TGGTATGCGC CCCCATCTTC GGCTATCTGG GCGATCGTTA CTCCCGCCCC 
TGGATAATGG CGGTGGGCGT GGGTCTGTGG AGCACCACCA CCCTACTGGG GTCGTTCATG 
AAGCAGTTTG GATGGTTCAT CGCCTTCAGA GCGCTGGTCG GGATCGGCGA GGCATCGTAC 
AGTACCATCG CACCGACGAT TATCTCGGAT CTGTTCGTGC ACGACATGCG CTCCAAGATG 
CTGGCACTGT TCTACTTTGC CATACCCGTG GGATCCGGTC TGGGATACAT TGTGGGATCC 
AAGACAGCGC ACCTGGCCAA CGATTGGCGC TGGGCTCTGA GGGTCACGCC CATTCTGGGC 
ATTGTGGCAG TTTTCCTCAT TTTGCTGATC AAGGATCCGG TGCGGGGTCA CAGCGAGGGA 
TCGCACAATT TGGAGGCCAC TACCTACAAG CAGGACATCA AGGCGTTGGT CAGGAACCGT 
TCCTTCATGC TCTCCACGGC TGGATTCACG TGCGTTGCCT TCGTGGCCGG AGCACTGGCC 
TGGTGGGGAC CTTCCTTCAT CTATCTAGGC ATGAAGATGC AGCCCGGCAA CGAGAACATT 
GTCCAGGACG ACATCTCGTA CAAGTTTGGT CTGGTGGCCA TGTTGGCCGG ACTCATCGGA 
GTCCCGTTGG GCTCCTTTCT GGCCCAGCGA TTGAGGGGTC GCTACGAGAA CTGTGATCCG 
TACATCTGTG CGGTGGGACT TTTCATCTCG GCTCCGATGG TCTTCGCTGC TCTCGTGGTG 
CCGCAGACAA GCGAGAGCCT CTGCTTTTTC TTCGTTTTCG TGGCCCAGGT GGCACTGAAC 
CTGTGCTGGT CCATTGTAGC CGACATCCTA CTGTATGTGG TGGTTCCCAC GCGACGTTCA 
ACAGCCGAGG CCTTCCAAAT CCTCATCTCA CACGCACTCG GTGATGCCGG CAGTCCGTAT 
TTGGTTGGAG CAATCTCCGA GGCCATCATG AAACACCTGC ACAAGAACCC CAGCGACTCT 
GGACTGACCA CCGAGCTGCG AAGCATGTCC CAAGTGGCAG GTTCCGCGAT CAGTAATGCC 
ACCCAGGTGA TCGCGGAAGC CACCACCAGT TTGATGGAAA CAGCACGCTC GTCAGCCTCT 
CAGGAATACT CTGATGTGGA GCAATTCGAG GGACTGCAAT ATGCGCTCTT CTCCACCAGT 
TTTGTGGAGG TGCTGGGCGG CATTTTCTTC ATATTCACCG CCTGCTTTAT CATCAAAGAC 
AAGTACAATG CCACACGAGG ACTGCAAGGC GATCAGGGAG CGCAGGCGGT TCGATCGTCA 
GTCGCCTTGG CCAGCGGGCA AAAGGACGTC GAGTCCTTCA ACTCGGACTG CCTGGTCCTT 
TGCACTGACA TCGCGCTGCG CGAACGCACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spin ( NM_166144.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725530.1 Drosophila melanogaster spinster (spin), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa11404
Clone ID Related Accession (Same CDS sequence)	NM_166144.2
Accession Version	NM_166144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spinster, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166144.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTGA AACACCAGAA GCAATCCTAC CAACCGCTCC CAACGGCGGC CGCCATGGAC 
AATCCGGCGA TGATCCAGAG CAGCGGTAGC AGTGGGAGCA GCTCCTCTGA GGAGGGCGGG 
AGTCGTGAGG ATGTGGCCAA TCTATCGCCG CTCGGACTGC CTACGACCTA CTCGTCACAG 
CAATTGATGC CCTCGGATAC GGACAGCATG GAGGAGGAGC GGCACCGACT GCGTCCCCAC 
CACCATCACC ACCACCCACT GGGCGAGCAC CACCACATCC CGGGCATACC ACCATCCGCC 
GTCGTGCCAT CGCGTCTCTC CAGCGTGGGC AGATCGCAGT GGTTCACCGT TACCGTGCTA 
TGCTTCGTCA ACCTCATCAA CTACATGGAT CGCTTTACGA TCGCAGGAGT TCTCACAGAT 
GTCCGAAATG ATTTCGATAT TGGCAATGAT AGTGCCGGAC TTCTCCAGAC GGTCTTCGTT 
ATCTCGTACA TGGTATGCGC CCCCATCTTC GGCTATCTGG GCGATCGTTA CTCCCGCCCC 
TGGATAATGG CGGTGGGCGT GGGTCTGTGG AGCACCACCA CCCTACTGGG GTCGTTCATG 
AAGCAGTTTG GATGGTTCAT CGCCTTCAGA GCGCTGGTCG GGATCGGCGA GGCATCGTAC 
AGTACCATCG CACCGACGAT TATCTCGGAT CTGTTCGTGC ACGACATGCG CTCCAAGATG 
CTGGCACTGT TCTACTTTGC CATACCCGTG GGATCCGGTC TGGGATACAT TGTGGGATCC 
AAGACAGCGC ACCTGGCCAA CGATTGGCGC TGGGCTCTGA GGGTCACGCC CATTCTGGGC 
ATTGTGGCAG TTTTCCTCAT TTTGCTGATC AAGGATCCGG TGCGGGGTCA CAGCGAGGGA 
TCGCACAATT TGGAGGCCAC TACCTACAAG CAGGACATCA AGGCGTTGGT CAGGAACCGT 
TCCTTCATGC TCTCCACGGC TGGATTCACG TGCGTTGCCT TCGTGGCCGG AGCACTGGCC 
TGGTGGGGAC CTTCCTTCAT CTATCTAGGC ATGAAGATGC AGCCCGGCAA CGAGAACATT 
GTCCAGGACG ACATCTCGTA CAAGTTTGGT CTGGTGGCCA TGTTGGCCGG ACTCATCGGA 
GTCCCGTTGG GCTCCTTTCT GGCCCAGCGA TTGAGGGGTC GCTACGAGAA CTGTGATCCG 
TACATCTGTG CGGTGGGACT TTTCATCTCG GCTCCGATGG TCTTCGCTGC TCTCGTGGTG 
CCGCAGACAA GCGAGAGCCT CTGCTTTTTC TTCGTTTTCG TGGCCCAGGT GGCACTGAAC 
CTGTGCTGGT CCATTGTAGC CGACATCCTA CTGTATGTGG TGGTTCCCAC GCGACGTTCA 
ACAGCCGAGG CCTTCCAAAT CCTCATCTCA CACGCACTCG GTGATGCCGG CAGTCCGTAT 
TTGGTTGGAG CAATCTCCGA GGCCATCATG AAACACCTGC ACAAGAACCC CAGCGACTCT 
GGACTGACCA CCGAGCTGCG AAGCATGTCC CAAGTGGCAG GTTCCGCGAT CAGTAATGCC 
ACCCAGGTGA TCGCGGAAGC CACCACCAGT TTGATGGAAA CAGCACGCTC GTCAGCCTCT 
CAGGAATACT CTGATGTGGA GCAATTCGAG GGACTGCAAT ATGCGCTCTT CTCCACCAGT 
TTTGTGGAGG TGCTGGGCGG CATTTTCTTC ATATTCACCG CCTGCTTTAT CATCAAAGAC 
AAGTACAATG CCACACGAGG ACTGCAAGAT GCCACGGCTC AGCAACAGCA GCGGGATGAG 
CGCGGTCAGA TTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725530.1
CDS	397..2214
Translation	MSLKHQKQSYQPLPTAAAMDNPAMIQSSGSSGSSSSEEGGSREDVANLSPLGLPTTYSSQQLMPSDTDSMEEERHRLRPHHHHHHPLGEHHHIPGIPPSAVVPSRLSSVGRSQWFTVTVLCFVNLINYMDRFTIAGVLTDVRNDFDIGNDSAGLLQTVFVISYMVCAPIFGYLGDRYSRPWIMAVGVGLWSTTTLLGSFMKQFGWFIAFRALVGIGEASYSTIAPTIISDLFVHDMRSKMLALFYFAIPVGSGLGYIVGSKTAHLANDWRWALRVTPILGIVAVFLILLIKDPVRGHSEGSHNLEATTYKQDIKALVRNRSFMLSTAGFTCVAFVAGALAWWGPSFIYLGMKMQPGNENIVQDDISYKFGLVAMLAGLIGVPLGSFLAQRLRGRYENCDPYICAVGLFISAPMVFAALVVPQTSESLCFFFVFVAQVALNLCWSIVADILLYVVVPTRRSTAEAFQILISHALGDAGSPYLVGAISEAIMKHLHKNPSDSGLTTELRSMSQVAGSAISNATQVIAEATTSLMETARSSASQEYSDVEQFEGLQYALFSTSFVEVLGGIFFIFTACFIIKDKYNATRGLQDATAQQQQRDERGQIA

Target ORF information:

RefSeq Version	NM_166144.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spinster (spin), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166144.2

ORF Insert Sequence:

ATGTCGCTGA AACACCAGAA GCAATCCTAC CAACCGCTCC CAACGGCGGC CGCCATGGAC 
AATCCGGCGA TGATCCAGAG CAGCGGTAGC AGTGGGAGCA GCTCCTCTGA GGAGGGCGGG 
AGTCGTGAGG ATGTGGCCAA TCTATCGCCG CTCGGACTGC CTACGACCTA CTCGTCACAG 
CAATTGATGC CCTCGGATAC GGACAGCATG GAGGAGGAGC GGCACCGACT GCGTCCCCAC 
CACCATCACC ACCACCCACT GGGCGAGCAC CACCACATCC CGGGCATACC ACCATCCGCC 
GTCGTGCCAT CGCGTCTCTC CAGCGTGGGC AGATCGCAGT GGTTCACCGT TACCGTGCTA 
TGCTTCGTCA ACCTCATCAA CTACATGGAT CGCTTTACGA TCGCAGGAGT TCTCACAGAT 
GTCCGAAATG ATTTCGATAT TGGCAATGAT AGTGCCGGAC TTCTCCAGAC GGTCTTCGTT 
ATCTCGTACA TGGTATGCGC CCCCATCTTC GGCTATCTGG GCGATCGTTA CTCCCGCCCC 
TGGATAATGG CGGTGGGCGT GGGTCTGTGG AGCACCACCA CCCTACTGGG GTCGTTCATG 
AAGCAGTTTG GATGGTTCAT CGCCTTCAGA GCGCTGGTCG GGATCGGCGA GGCATCGTAC 
AGTACCATCG CACCGACGAT TATCTCGGAT CTGTTCGTGC ACGACATGCG CTCCAAGATG 
CTGGCACTGT TCTACTTTGC CATACCCGTG GGATCCGGTC TGGGATACAT TGTGGGATCC 
AAGACAGCGC ACCTGGCCAA CGATTGGCGC TGGGCTCTGA GGGTCACGCC CATTCTGGGC 
ATTGTGGCAG TTTTCCTCAT TTTGCTGATC AAGGATCCGG TGCGGGGTCA CAGCGAGGGA 
TCGCACAATT TGGAGGCCAC TACCTACAAG CAGGACATCA AGGCGTTGGT CAGGAACCGT 
TCCTTCATGC TCTCCACGGC TGGATTCACG TGCGTTGCCT TCGTGGCCGG AGCACTGGCC 
TGGTGGGGAC CTTCCTTCAT CTATCTAGGC ATGAAGATGC AGCCCGGCAA CGAGAACATT 
GTCCAGGACG ACATCTCGTA CAAGTTTGGT CTGGTGGCCA TGTTGGCCGG ACTCATCGGA 
GTCCCGTTGG GCTCCTTTCT GGCCCAGCGA TTGAGGGGTC GCTACGAGAA CTGTGATCCG 
TACATCTGTG CGGTGGGACT TTTCATCTCG GCTCCGATGG TCTTCGCTGC TCTCGTGGTG 
CCGCAGACAA GCGAGAGCCT CTGCTTTTTC TTCGTTTTCG TGGCCCAGGT GGCACTGAAC 
CTGTGCTGGT CCATTGTAGC CGACATCCTA CTGTATGTGG TGGTTCCCAC GCGACGTTCA 
ACAGCCGAGG CCTTCCAAAT CCTCATCTCA CACGCACTCG GTGATGCCGG CAGTCCGTAT 
TTGGTTGGAG CAATCTCCGA GGCCATCATG AAACACCTGC ACAAGAACCC CAGCGACTCT 
GGACTGACCA CCGAGCTGCG AAGCATGTCC CAAGTGGCAG GTTCCGCGAT CAGTAATGCC 
ACCCAGGTGA TCGCGGAAGC CACCACCAGT TTGATGGAAA CAGCACGCTC GTCAGCCTCT 
CAGGAATACT CTGATGTGGA GCAATTCGAG GGACTGCAAT ATGCGCTCTT CTCCACCAGT 
TTTGTGGAGG TGCTGGGCGG CATTTTCTTC ATATTCACCG CCTGCTTTAT CATCAAAGAC 
AAGTACAATG CCACACGAGG ACTGCAAGAT GCCACGGCTC AGCAACAGCA GCGGGATGAG 
CGCGGTCAGA TTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spin ( NM_080084.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524823.1 Drosophila melanogaster spinster (spin), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa11405
Clone ID Related Accession (Same CDS sequence)	NM_080084.3
Accession Version	NM_080084.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spinster, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_080084.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTGA AACACCAGAA GCAATCCTAC CAACCGCTCC CAACGGCGGC CGCCATGGAC 
AATCCGGCGA TGATCCAGAG CAGCGGTAGC AGTGGGAGCA GCTCCTCTGA GGAGGGCGGG 
AGTCGTGAGG ATGTGGCCAA TCTATCGCCG CTCGGACTGC CTACGACCTA CTCGTCACAG 
CAATTGATGC CCTCGGATAC GGACAGCATG GAGGAGGAGC GGCACCGACT GCGTCCCCAC 
CACCATCACC ACCACCCACT GGGCGAGCAC CACCACATCC CGGGCATACC ACCATCCGCC 
GTCGTGCCAT CGCGTCTCTC CAGCGTGGGC AGATCGCAGT GGTTCACCGT TACCGTGCTA 
TGCTTCGTCA ACCTCATCAA CTACATGGAT CGCTTTACGA TCGCAGGAGT TCTCACAGAT 
GTCCGAAATG ATTTCGATAT TGGCAATGAT AGTGCCGGAC TTCTCCAGAC GGTCTTCGTT 
ATCTCGTACA TGGTATGCGC CCCCATCTTC GGCTATCTGG GCGATCGTTA CTCCCGCCCC 
TGGATAATGG CGGTGGGCGT GGGTCTGTGG AGCACCACCA CCCTACTGGG GTCGTTCATG 
AAGCAGTTTG GATGGTTCAT CGCCTTCAGA GCGCTGGTCG GGATCGGCGA GGCATCGTAC 
AGTACCATCG CACCGACGAT TATCTCGGAT CTGTTCGTGC ACGACATGCG CTCCAAGATG 
CTGGCACTGT TCTACTTTGC CATACCCGTG GGATCCGGTC TGGGATACAT TGTGGGATCC 
AAGACAGCGC ACCTGGCCAA CGATTGGCGC TGGGCTCTGA GGGTCACGCC CATTCTGGGC 
ATTGTGGCAG TTTTCCTCAT TTTGCTGATC AAGGATCCGG TGCGGGGTCA CAGCGAGGGA 
TCGCACAATT TGGAGGCCAC TACCTACAAG CAGGACATCA AGGCGTTGGT CAGGAACCGT 
TCCTTCATGC TCTCCACGGC TGGATTCACG TGCGTTGCCT TCGTGGCCGG AGCACTGGCC 
TGGTGGGGAC CTTCCTTCAT CTATCTAGGC ATGAAGATGC AGCCCGGCAA CGAGAACATT 
GTCCAGGACG ATGTTGCGTT CAACTTCGGA GTCATCACCA TGTTGGCGGG CCTGCTGGGC 
GTGCCGCTGG GCTCGTTCCT GTCCCAGTAC CTGGTTAAGC GTTATCCCAC GGCGGATCCG 
GTAATCTGCG CCTTTGGGCT GCTCGTGAGT GCGCCGCTGC TGACGGGCGC CTGTCTCTTG 
GTCAACAGCA ATTCGGTTGG CACCTATGCA CTCATATTTT TCGGGCAGTT AGCTTTAAAT 
TTGAATTGGG CAATTGTTGC GGACATACTG CTGTATGTGG TGGTTCCCAC GCGACGTTCA 
ACAGCCGAGG CCTTCCAAAT CCTCATCTCA CACGCACTCG GTGATGCCGG CAGTCCGTAT 
TTGGTTGGAG CAATCTCCGA GGCCATCATG AAACACCTGC ACAAGAACCC CAGCGACTCT 
GGACTGACCA CCGAGCTGCG AAGCATGTCC CAAGTGGCAG GTTCCGCGAT CAGTAATGCC 
ACCCAGGTGA TCGCGGAAGC CACCACCAGT TTGATGGAAA CAGCACGCTC GTCAGCCTCT 
CAGGAATACT CTGATGTGGA GCAATTCGAG GGACTGCAAT ATGCGCTCTT CTCCACCAGT 
TTTGTGGAGG TGCTGGGCGG CATTTTCTTC ATATTCACCG CCTGCTTTAT CATCAAAGAC 
AAGTACAATG CCACACGAGG ACTGCAAGGC GATCAGGGAG CGCAGGCGGT TCGATCGTCA 
GTCGCCTTGG CCAGCGGGCA AAAGGACGTC GAGTCCTTCA ACTCGGACTG CCTGGTCCTT 
TGCACTGACA TCGCGCTGCG CGAACGCACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524823.1
CDS	397..2289
Translation	MSLKHQKQSYQPLPTAAAMDNPAMIQSSGSSGSSSSEEGGSREDVANLSPLGLPTTYSSQQLMPSDTDSMEEERHRLRPHHHHHHPLGEHHHIPGIPPSAVVPSRLSSVGRSQWFTVTVLCFVNLINYMDRFTIAGVLTDVRNDFDIGNDSAGLLQTVFVISYMVCAPIFGYLGDRYSRPWIMAVGVGLWSTTTLLGSFMKQFGWFIAFRALVGIGEASYSTIAPTIISDLFVHDMRSKMLALFYFAIPVGSGLGYIVGSKTAHLANDWRWALRVTPILGIVAVFLILLIKDPVRGHSEGSHNLEATTYKQDIKALVRNRSFMLSTAGFTCVAFVAGALAWWGPSFIYLGMKMQPGNENIVQDDVAFNFGVITMLAGLLGVPLGSFLSQYLVKRYPTADPVICAFGLLVSAPLLTGACLLVNSNSVGTYALIFFGQLALNLNWAIVADILLYVVVPTRRSTAEAFQILISHALGDAGSPYLVGAISEAIMKHLHKNPSDSGLTTELRSMSQVAGSAISNATQVIAEATTSLMETARSSASQEYSDVEQFEGLQYALFSTSFVEVLGGIFFIFTACFIIKDKYNATRGLQGDQGAQAVRSSVALASGQKDVESFNSDCLVLCTDIALRERT

Target ORF information:

RefSeq Version	NM_080084.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spinster (spin), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080084.3

ORF Insert Sequence:

ATGTCGCTGA AACACCAGAA GCAATCCTAC CAACCGCTCC CAACGGCGGC CGCCATGGAC 
AATCCGGCGA TGATCCAGAG CAGCGGTAGC AGTGGGAGCA GCTCCTCTGA GGAGGGCGGG 
AGTCGTGAGG ATGTGGCCAA TCTATCGCCG CTCGGACTGC CTACGACCTA CTCGTCACAG 
CAATTGATGC CCTCGGATAC GGACAGCATG GAGGAGGAGC GGCACCGACT GCGTCCCCAC 
CACCATCACC ACCACCCACT GGGCGAGCAC CACCACATCC CGGGCATACC ACCATCCGCC 
GTCGTGCCAT CGCGTCTCTC CAGCGTGGGC AGATCGCAGT GGTTCACCGT TACCGTGCTA 
TGCTTCGTCA ACCTCATCAA CTACATGGAT CGCTTTACGA TCGCAGGAGT TCTCACAGAT 
GTCCGAAATG ATTTCGATAT TGGCAATGAT AGTGCCGGAC TTCTCCAGAC GGTCTTCGTT 
ATCTCGTACA TGGTATGCGC CCCCATCTTC GGCTATCTGG GCGATCGTTA CTCCCGCCCC 
TGGATAATGG CGGTGGGCGT GGGTCTGTGG AGCACCACCA CCCTACTGGG GTCGTTCATG 
AAGCAGTTTG GATGGTTCAT CGCCTTCAGA GCGCTGGTCG GGATCGGCGA GGCATCGTAC 
AGTACCATCG CACCGACGAT TATCTCGGAT CTGTTCGTGC ACGACATGCG CTCCAAGATG 
CTGGCACTGT TCTACTTTGC CATACCCGTG GGATCCGGTC TGGGATACAT TGTGGGATCC 
AAGACAGCGC ACCTGGCCAA CGATTGGCGC TGGGCTCTGA GGGTCACGCC CATTCTGGGC 
ATTGTGGCAG TTTTCCTCAT TTTGCTGATC AAGGATCCGG TGCGGGGTCA CAGCGAGGGA 
TCGCACAATT TGGAGGCCAC TACCTACAAG CAGGACATCA AGGCGTTGGT CAGGAACCGT 
TCCTTCATGC TCTCCACGGC TGGATTCACG TGCGTTGCCT TCGTGGCCGG AGCACTGGCC 
TGGTGGGGAC CTTCCTTCAT CTATCTAGGC ATGAAGATGC AGCCCGGCAA CGAGAACATT 
GTCCAGGACG ATGTTGCGTT CAACTTCGGA GTCATCACCA TGTTGGCGGG CCTGCTGGGC 
GTGCCGCTGG GCTCGTTCCT GTCCCAGTAC CTGGTTAAGC GTTATCCCAC GGCGGATCCG 
GTAATCTGCG CCTTTGGGCT GCTCGTGAGT GCGCCGCTGC TGACGGGCGC CTGTCTCTTG 
GTCAACAGCA ATTCGGTTGG CACCTATGCA CTCATATTTT TCGGGCAGTT AGCTTTAAAT 
TTGAATTGGG CAATTGTTGC GGACATACTG CTGTATGTGG TGGTTCCCAC GCGACGTTCA 
ACAGCCGAGG CCTTCCAAAT CCTCATCTCA CACGCACTCG GTGATGCCGG CAGTCCGTAT 
TTGGTTGGAG CAATCTCCGA GGCCATCATG AAACACCTGC ACAAGAACCC CAGCGACTCT 
GGACTGACCA CCGAGCTGCG AAGCATGTCC CAAGTGGCAG GTTCCGCGAT CAGTAATGCC 
ACCCAGGTGA TCGCGGAAGC CACCACCAGT TTGATGGAAA CAGCACGCTC GTCAGCCTCT 
CAGGAATACT CTGATGTGGA GCAATTCGAG GGACTGCAAT ATGCGCTCTT CTCCACCAGT 
TTTGTGGAGG TGCTGGGCGG CATTTTCTTC ATATTCACCG CCTGCTTTAT CATCAAAGAC 
AAGTACAATG CCACACGAGG ACTGCAAGGC GATCAGGGAG CGCAGGCGGT TCGATCGTCA 
GTCGCCTTGG CCAGCGGGCA AAAGGACGTC GAGTCCTTCA ACTCGGACTG CCTGGTCCTT 
TGCACTGACA TCGCGCTGCG CGAACGCACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spin ( NM_166145.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725531.1 Drosophila melanogaster spinster (spin), transcript variant A, mRNA.

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CloneID	OFa11406
Clone ID Related Accession (Same CDS sequence)	NM_166145.2
Accession Version	NM_166145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spinster, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166145.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTGA AACACCAGAA GCAATCCTAC CAACCGCTCC CAACGGCGGC CGCCATGGAC 
AATCCGGCGA TGATCCAGAG CAGCGGTAGC AGTGGGAGCA GCTCCTCTGA GGAGGGCGGG 
AGTCGTGAGG ATGTGGCCAA TCTATCGCCG CTCGGACTGC CTACGACCTA CTCGTCACAG 
CAATTGATGC CCTCGGATAC GGACAGCATG GAGGAGGAGC GGCACCGACT GCGTCCCCAC 
CACCATCACC ACCACCCACT GGGCGAGCAC CACCACATCC CGGGCATACC ACCATCCGCC 
GTCGTGCCAT CGCGTCTCTC CAGCGTGGGC AGATCGCAGT GGTTCACCGT TACCGTGCTA 
TGCTTCGTCA ACCTCATCAA CTACATGGAT CGCTTTACGA TCGCAGGAGT TCTCACAGAT 
GTCCGAAATG ATTTCGATAT TGGCAATGAT AGTGCCGGAC TTCTCCAGAC GGTCTTCGTT 
ATCTCGTACA TGGTATGCGC CCCCATCTTC GGCTATCTGG GCGATCGTTA CTCCCGCCCC 
TGGATAATGG CGGTGGGCGT GGGTCTGTGG AGCACCACCA CCCTACTGGG GTCGTTCATG 
AAGCAGTTTG GATGGTTCAT CGCCTTCAGA GCGCTGGTCG GGATCGGCGA GGCATCGTAC 
AGTACCATCG CACCGACGAT TATCTCGGAT CTGTTCGTGC ACGACATGCG CTCCAAGATG 
CTGGCACTGT TCTACTTTGC CATACCCGTG GGATCCGGTC TGGGATACAT TGTGGGATCC 
AAGACAGCGC ACCTGGCCAA CGATTGGCGC TGGGCTCTGA GGGTCACGCC CATTCTGGGC 
ATTGTGGCAG TTTTCCTCAT TTTGCTGATC AAGGATCCGG TGCGGGGTCA CAGCGAGGGA 
TCGCACAATT TGGAGGCCAC TACCTACAAG CAGGACATCA AGGCGTTGGT CAGGAACCGT 
TCCTTCATGC TCTCCACGGC TGGATTCACG TGCGTTGCCT TCGTGGCCGG AGCACTGGCC 
TGGTGGGGAC CTTCCTTCAT CTATCTAGGC ATGAAGATGC AGCCCGGCAA CGAGAACATT 
GTCCAGGACG ATGTTGCGTT CAACTTCGGA GTCATCACCA TGTTGGCGGG CCTGCTGGGC 
GTGCCGCTGG GCTCGTTCCT GTCCCAGTAC CTGGTTAAGC GTTATCCCAC GGCGGATCCG 
GTAATCTGCG CCTTTGGGCT GCTCGTGAGT GCGCCGCTGC TGACGGGCGC CTGTCTCTTG 
GTCAACAGCA ATTCGGTTGG CACCTATGCA CTCATATTTT TCGGGCAGTT AGCTTTAAAT 
TTGAATTGGG CAATTGTTGC GGACATACTG CTGTATGTGG TGGTTCCCAC GCGACGTTCA 
ACAGCCGAGG CCTTCCAAAT CCTCATCTCA CACGCACTCG GTGATGCCGG CAGTCCGTAT 
TTGGTTGGAG CAATCTCCGA GGCCATCATG AAACACCTGC ACAAGAACCC CAGCGACTCT 
GGACTGACCA CCGAGCTGCG AAGCATGTCC CAAGTGGCAG GTTCCGCGAT CAGTAATGCC 
ACCCAGGTGA TCGCGGAAGC CACCACCAGT TTGATGGAAA CAGCACGCTC GTCAGCCTCT 
CAGGAATACT CTGATGTGGA GCAATTCGAG GGACTGCAAT ATGCGCTCTT CTCCACCAGT 
TTTGTGGAGG TGCTGGGCGG CATTTTCTTC ATATTCACCG CCTGCTTTAT CATCAAAGAC 
AAGTACAATG CCACACGAGG ACTGCAAGAT GCCACGGCTC AGCAACAGCA GCGGGATGAG 
CGCGGTCAGA TTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725531.1
CDS	397..2214
Translation	MSLKHQKQSYQPLPTAAAMDNPAMIQSSGSSGSSSSEEGGSREDVANLSPLGLPTTYSSQQLMPSDTDSMEEERHRLRPHHHHHHPLGEHHHIPGIPPSAVVPSRLSSVGRSQWFTVTVLCFVNLINYMDRFTIAGVLTDVRNDFDIGNDSAGLLQTVFVISYMVCAPIFGYLGDRYSRPWIMAVGVGLWSTTTLLGSFMKQFGWFIAFRALVGIGEASYSTIAPTIISDLFVHDMRSKMLALFYFAIPVGSGLGYIVGSKTAHLANDWRWALRVTPILGIVAVFLILLIKDPVRGHSEGSHNLEATTYKQDIKALVRNRSFMLSTAGFTCVAFVAGALAWWGPSFIYLGMKMQPGNENIVQDDVAFNFGVITMLAGLLGVPLGSFLSQYLVKRYPTADPVICAFGLLVSAPLLTGACLLVNSNSVGTYALIFFGQLALNLNWAIVADILLYVVVPTRRSTAEAFQILISHALGDAGSPYLVGAISEAIMKHLHKNPSDSGLTTELRSMSQVAGSAISNATQVIAEATTSLMETARSSASQEYSDVEQFEGLQYALFSTSFVEVLGGIFFIFTACFIIKDKYNATRGLQDATAQQQQRDERGQIA

Target ORF information:

RefSeq Version	NM_166145.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spinster (spin), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166145.2

ORF Insert Sequence:

ATGTCGCTGA AACACCAGAA GCAATCCTAC CAACCGCTCC CAACGGCGGC CGCCATGGAC 
AATCCGGCGA TGATCCAGAG CAGCGGTAGC AGTGGGAGCA GCTCCTCTGA GGAGGGCGGG 
AGTCGTGAGG ATGTGGCCAA TCTATCGCCG CTCGGACTGC CTACGACCTA CTCGTCACAG 
CAATTGATGC CCTCGGATAC GGACAGCATG GAGGAGGAGC GGCACCGACT GCGTCCCCAC 
CACCATCACC ACCACCCACT GGGCGAGCAC CACCACATCC CGGGCATACC ACCATCCGCC 
GTCGTGCCAT CGCGTCTCTC CAGCGTGGGC AGATCGCAGT GGTTCACCGT TACCGTGCTA 
TGCTTCGTCA ACCTCATCAA CTACATGGAT CGCTTTACGA TCGCAGGAGT TCTCACAGAT 
GTCCGAAATG ATTTCGATAT TGGCAATGAT AGTGCCGGAC TTCTCCAGAC GGTCTTCGTT 
ATCTCGTACA TGGTATGCGC CCCCATCTTC GGCTATCTGG GCGATCGTTA CTCCCGCCCC 
TGGATAATGG CGGTGGGCGT GGGTCTGTGG AGCACCACCA CCCTACTGGG GTCGTTCATG 
AAGCAGTTTG GATGGTTCAT CGCCTTCAGA GCGCTGGTCG GGATCGGCGA GGCATCGTAC 
AGTACCATCG CACCGACGAT TATCTCGGAT CTGTTCGTGC ACGACATGCG CTCCAAGATG 
CTGGCACTGT TCTACTTTGC CATACCCGTG GGATCCGGTC TGGGATACAT TGTGGGATCC 
AAGACAGCGC ACCTGGCCAA CGATTGGCGC TGGGCTCTGA GGGTCACGCC CATTCTGGGC 
ATTGTGGCAG TTTTCCTCAT TTTGCTGATC AAGGATCCGG TGCGGGGTCA CAGCGAGGGA 
TCGCACAATT TGGAGGCCAC TACCTACAAG CAGGACATCA AGGCGTTGGT CAGGAACCGT 
TCCTTCATGC TCTCCACGGC TGGATTCACG TGCGTTGCCT TCGTGGCCGG AGCACTGGCC 
TGGTGGGGAC CTTCCTTCAT CTATCTAGGC ATGAAGATGC AGCCCGGCAA CGAGAACATT 
GTCCAGGACG ATGTTGCGTT CAACTTCGGA GTCATCACCA TGTTGGCGGG CCTGCTGGGC 
GTGCCGCTGG GCTCGTTCCT GTCCCAGTAC CTGGTTAAGC GTTATCCCAC GGCGGATCCG 
GTAATCTGCG CCTTTGGGCT GCTCGTGAGT GCGCCGCTGC TGACGGGCGC CTGTCTCTTG 
GTCAACAGCA ATTCGGTTGG CACCTATGCA CTCATATTTT TCGGGCAGTT AGCTTTAAAT 
TTGAATTGGG CAATTGTTGC GGACATACTG CTGTATGTGG TGGTTCCCAC GCGACGTTCA 
ACAGCCGAGG CCTTCCAAAT CCTCATCTCA CACGCACTCG GTGATGCCGG CAGTCCGTAT 
TTGGTTGGAG CAATCTCCGA GGCCATCATG AAACACCTGC ACAAGAACCC CAGCGACTCT 
GGACTGACCA CCGAGCTGCG AAGCATGTCC CAAGTGGCAG GTTCCGCGAT CAGTAATGCC 
ACCCAGGTGA TCGCGGAAGC CACCACCAGT TTGATGGAAA CAGCACGCTC GTCAGCCTCT 
CAGGAATACT CTGATGTGGA GCAATTCGAG GGACTGCAAT ATGCGCTCTT CTCCACCAGT 
TTTGTGGAGG TGCTGGGCGG CATTTTCTTC ATATTCACCG CCTGCTTTAT CATCAAAGAC 
AAGTACAATG CCACACGAGG ACTGCAAGAT GCCACGGCTC AGCAACAGCA GCGGGATGAG 
CGCGGTCAGA TTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spin ( NM_166147.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725533.1 Drosophila melanogaster spinster (spin), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa11407
Clone ID Related Accession (Same CDS sequence)	NM_166147.2
Accession Version	NM_166147.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spinster, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166147.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTGA AACACCAGAA GCAATCCTAC CAACCGCTCC CAACGGCGGC CGCCATGGAC 
AATCCGGCGA TGATCCAGAG CAGCGGTAGC AGTGGGAGCA GCTCCTCTGA GGAGGGCGGG 
AGTCGTGAGG ATGTGGCCAA TCTATCGCCG CTCGGACTGC CTACGACCTA CTCGTCACAG 
CAATTGATGC CCTCGGATAC GGACAGCATG GAGGAGGAGC GGCACCGACT GCGTCCCCAC 
CACCATCACC ACCACCCACT GGGCGAGCAC CACCACATCC CGGGCATACC ACCATCCGCC 
GTCGTGCCAT CGCGTCTCTC CAGCGTGGGC AGATCGCAGT GGTTCACCGT TACCGTGCTA 
TGCTTCGTCA ACCTCATCAA CTACATGGAT CGCTTTACGA TCGCAGGAGT TCTCACAGAT 
GTCCGAAATG ATTTCGATAT TGGCAATGAT AGTGCCGGAC TTCTCCAGAC GGTCTTCGTT 
ATCTCGTACA TGGTATGCGC CCCCATCTTC GGCTATCTGG GCGATCGTTA CTCCCGCCCC 
TGGATAATGG CGGTGGGCGT GGGTCTGTGG AGCACCACCA CCCTACTGGG GTCGTTCATG 
AAGCAGTTTG GATGGTTCAT CGCCTTCAGA GCGCTGGTCG GGATCGGCGA GGCATCGTAC 
AGTACCATCG CACCGACGAT TATCTCGGAT CTGTTCGTGC ACGACATGCG CTCCAAGATG 
CTGGCACTGT TCTACTTTGC CATACCCGTG GGATCCGGTC TGGGATACAT TGTGGGATCC 
AAGACAGCGC ACCTGGCCAA CGATTGGCGC TGGGCTCTGA GGGTCACGCC CATTCTGGGC 
ATTGTGGCAG TTTTCCTCAT TTTGCTGATC AAGGATCCGG TGCGGGGTCA CAGCGAGGGA 
TCGCACAATT TGGAGGCCAC TACCTACAAG CAGGACATCA AGGCGTTGGT CAGGAACCGT 
TCCTTCATGC TCTCCACGGC TGGATTCACG TGCGTTGCCT TCGTGGCCGG AGCACTGGCC 
TGGTGGGGAC CTTCCTTCAT CTATCTAGGC ATGAAGATGC AGCCCGGCAA CGAGAACATT 
GTCCAGGACG ATATGTGGTG GTTCCCACGC GACGTTCAAC AGCCGAGGCC TTCCAAATCC 
TCATCTCACA CGCACTCGGT GATGCCGGCA GTCCGTATTT GGTTGGAGCA ATCTCCGAGG 
CCATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725533.1
CDS	397..1605
Translation	MSLKHQKQSYQPLPTAAAMDNPAMIQSSGSSGSSSSEEGGSREDVANLSPLGLPTTYSSQQLMPSDTDSMEEERHRLRPHHHHHHPLGEHHHIPGIPPSAVVPSRLSSVGRSQWFTVTVLCFVNLINYMDRFTIAGVLTDVRNDFDIGNDSAGLLQTVFVISYMVCAPIFGYLGDRYSRPWIMAVGVGLWSTTTLLGSFMKQFGWFIAFRALVGIGEASYSTIAPTIISDLFVHDMRSKMLALFYFAIPVGSGLGYIVGSKTAHLANDWRWALRVTPILGIVAVFLILLIKDPVRGHSEGSHNLEATTYKQDIKALVRNRSFMLSTAGFTCVAFVAGALAWWGPSFIYLGMKMQPGNENIVQDDMWWFPRDVQQPRPSKSSSHTHSVMPAVRIWLEQSPRPS

Target ORF information:

RefSeq Version	NM_166147.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spinster (spin), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166147.2

ORF Insert Sequence:

ATGTCGCTGA AACACCAGAA GCAATCCTAC CAACCGCTCC CAACGGCGGC CGCCATGGAC 
AATCCGGCGA TGATCCAGAG CAGCGGTAGC AGTGGGAGCA GCTCCTCTGA GGAGGGCGGG 
AGTCGTGAGG ATGTGGCCAA TCTATCGCCG CTCGGACTGC CTACGACCTA CTCGTCACAG 
CAATTGATGC CCTCGGATAC GGACAGCATG GAGGAGGAGC GGCACCGACT GCGTCCCCAC 
CACCATCACC ACCACCCACT GGGCGAGCAC CACCACATCC CGGGCATACC ACCATCCGCC 
GTCGTGCCAT CGCGTCTCTC CAGCGTGGGC AGATCGCAGT GGTTCACCGT TACCGTGCTA 
TGCTTCGTCA ACCTCATCAA CTACATGGAT CGCTTTACGA TCGCAGGAGT TCTCACAGAT 
GTCCGAAATG ATTTCGATAT TGGCAATGAT AGTGCCGGAC TTCTCCAGAC GGTCTTCGTT 
ATCTCGTACA TGGTATGCGC CCCCATCTTC GGCTATCTGG GCGATCGTTA CTCCCGCCCC 
TGGATAATGG CGGTGGGCGT GGGTCTGTGG AGCACCACCA CCCTACTGGG GTCGTTCATG 
AAGCAGTTTG GATGGTTCAT CGCCTTCAGA GCGCTGGTCG GGATCGGCGA GGCATCGTAC 
AGTACCATCG CACCGACGAT TATCTCGGAT CTGTTCGTGC ACGACATGCG CTCCAAGATG 
CTGGCACTGT TCTACTTTGC CATACCCGTG GGATCCGGTC TGGGATACAT TGTGGGATCC 
AAGACAGCGC ACCTGGCCAA CGATTGGCGC TGGGCTCTGA GGGTCACGCC CATTCTGGGC 
ATTGTGGCAG TTTTCCTCAT TTTGCTGATC AAGGATCCGG TGCGGGGTCA CAGCGAGGGA 
TCGCACAATT TGGAGGCCAC TACCTACAAG CAGGACATCA AGGCGTTGGT CAGGAACCGT 
TCCTTCATGC TCTCCACGGC TGGATTCACG TGCGTTGCCT TCGTGGCCGG AGCACTGGCC 
TGGTGGGGAC CTTCCTTCAT CTATCTAGGC ATGAAGATGC AGCCCGGCAA CGAGAACATT 
GTCCAGGACG ATATGTGGTG GTTCCCACGC GACGTTCAAC AGCCGAGGCC TTCCAAATCC 
TCATCTCACA CGCACTCGGT GATGCCGGCA GTCCGTATTT GGTTGGAGCA ATCTCCGAGG 
CCATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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