fwd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fwd
Entrez Gene ID	45374
Full Name	four wheel drive
Synonyms	CG7004,Dmel\CG7004,Fwd,PI4K,anon-WO0118547.259
General protein information	Preferred Names four wheel drive Names CG7004 gene product from transcript CG7004-RA CG7004-PA CG7004-PB CG7004-PC CG7004-PD Four-wheel drive fwd-PA fwd-PB fwd-PC fwd-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61C1-61C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_167826.2	NP_728518.1	four wheel drive, isoform B
NM_001370043.1	NP_001356971.1	four wheel drive, isoform E
NM_167827.2	NP_728519.1	four wheel drive, isoform C
NM_001274274.1	NP_001261203.1	four wheel drive, isoform D
NM_080083.4	NP_524822.1	four wheel drive, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	PI4KB	NP_002642.1
	PI4KB	XP_001106422.2
Pan troglodytes (chimpanzee)	PI4KB	XP_001171634.2
Danio rerio (zebrafish)	pi4kb	NP_001030149.2
Xenopus tropicalis (tropical clawed frog)	pi4kb	NP_001096204.1
Bos taurus (cattle)	PI4KB	NP_777208.1
Gallus gallus (chicken)	LOC100859723	XP_003642722.2
Gallus gallus (chicken)	PI4KB	XP_423394.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007512	XP_001687908.1
Caenorhabditis elegans (roundworm)	pifk-1	NP_508177.2
Canis lupus familiaris (dog)	PI4KB	XP_540314.3
Mus musculus (house mouse)	Pi4kb	NP_780565.2
Rattus norvegicus (Norway rat)	Pi4kb	NP_112345.1
Drosophila melanogaster (fruit fly)	fwd	NP_728518.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dPIP4K activity is a regulator of cell growth and TOR signaling during larval development
Title: Phosphatidylinositol 5-phosphate 4-kinase (PIP4K) regulates TOR signaling and cell growth during Drosophila development.

data suggest Fwd plays catalytic and noncatalytic roles in regulating Rab11 during cytokinesis
Title: Dual roles for the Drosophila PI 4-kinase four wheel drive in localizing Rab11 during cytokinesis.

The following fwd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fwd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa87755	NM_001370043.1 Latest version!	Drosophila melanogaster four wheel drive (fwd), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFa19402	NM_080083.4 Latest version!	Drosophila melanogaster four wheel drive (fwd), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa19402	NM_001274274.1 Latest version!	Drosophila melanogaster four wheel drive (fwd), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa19402	NM_167826.2 Latest version!	Drosophila melanogaster four wheel drive (fwd), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa19403	NM_167827.2 Latest version!	Drosophila melanogaster four wheel drive (fwd), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fwd ( NM_001370043.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356971.1 Drosophila melanogaster four wheel drive (fwd), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa87755
Clone ID Related Accession (Same CDS sequence)	NM_001370043.1
Accession Version	NM_001370043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	four wheel drive, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCCG TAATATCAGC ATTTAAAGGA AAAAAAAATA GCAATCCATC CTGCAATTCT 
CCAATGGGAT CGGCGAATGG CACTGCTCCG GTGGAGGCAA AGCCAAACAA TTGCTACCAG 
TACCTGCGCC ACATACGCGA AATCGATACA AACAACAAGC TCCTTTCTCC CGTCGTGGTT 
GTTGCAGAGG AGAGGCCAGT GAGCAAGGCT GGTTCGCCCT CGACAGTGGC GACCATGGCT 
ACTGGAGTCG ATGTGGTGGA TCACTGCCTG CCCACAACGG TACGATTGTC CGTTACCGAG 
GCGCTATCCG ACTTGAACAT GAACGGAAAA GCCGTCGAAC CCCCCGTCAC CCCATGCACT 
TCGGTTGGCG ACAATGCCAA TCTGGAGGTG GCTGAAACAT GGGCTTGTCC ACCGCCACAA 
GCGACGGATG ACGAGGACAT ATCCGAGCAC TCGACAGCCG CCTGCACCAG CACAAGTAGC 
TCCAGCGAGC ACAGCAACAG CACCGTGGTG CACAGTCCAA CTGTGGGGGA GAGCACCTGT 
GACTCCGTGT CCGTCTCGGT AAGCCATCCA AGTCCCAGCG TGAGTCAGAA CAACACATCG 
TCCGCCACCG GCCGGATGAG TAGCCCCCAC AGCCAGTCGT CGCTCTCCAT AGCCTGCGAG 
CCGCACTCGG CGTCGACCAC CACGCTGTCG TCGCTGGGAT CAGACCTGGG CAATGGAAAC 
GGAAGTACCG TACCCGCGTT TCTAATGGCC GCCCCCGTCA CGCTGCCAAT TGGCGGCCAG 
ATGCCCACGA TAAAGTGCCT CCCAGGAGCA AGCTCAGCGG CCAGCACGCC CAAGCACTCG 
CGCTACTCTA AGCCTCGTCT CAGCCTGAGC CGCGTGTTTA ATCACTCTAT GCCCTCCGTG 
CACGGCCGAC CAAATCTCAG TTTAGCGCCA GCTGGAGTTC CGGGAAGTGT GAATCCGCCG 
AAACGTATTT CCACGCACCA AAGAAACCTA AGCTTGGATT TTAGATCCAT GGGCATACTT 
CTTCCGCCTG TCTCGCAGGT GACCAATAAT CACATCAATC ACACCCAGCA CCACCGCAAT 
CGCAGCCTCG ACAGTGCCTT GCAACGCATT CCGGAGGTCG AAGTGTCATC GCCAAATGCT 
GAAAGCGAAA ACACGTTCTG CTCACCGTCC ATTCTCGGTG CGACTATGTG CTCAAAAATT 
GCCACGACAG CGGCAACTGT AACGTCAGCC CTGTCCCCAC CAGGCACGCC CACACCGGCT 
CTGATGTCGA CGGCGTGCAC GCCCACGATT GGAGCCGTAA AGGGCAACGT GCAACATCCC 
GTGCCGAAGC ATGAGCAATC GATTTCTTTG GCAGCTGCTT GCAGGTCCAG TGCCGTCGTG 
GAATCCTGTC CAACGTTACG AATGCGCCCA AAATCCAGCG AGTCGGTTGC GGCCAATGGA 
CAAGAGATGA TCGGCGTCAT TGTCAGCAGC ACGGGCAACG GCAGTAGCAG CAGTAATAAT 
AGCTGTAGTA GCGCTGGTAG CACCAAGAAG CGAGAGGATC TCACCAGTCT TGGATCGGAT 
GATTCTGGCA TCATCTGTGG CTCAGAGTCG GACCAAATAT CTCTGAATCG CATATGCCAC 
TCACATGAGT CGCTTGATTC GGGTGAGATG GATGCGGATG CCGATGCAGA AGAGGAGTGC 
GTTGATCTTA TGGATACGAC ATCCATCGAC GAGGAGTACA ACATTATGCA GCCGGACCAT 
CTCTGCCTCT ATCCTCCACA GGCCGCTTCC ACCGAATTGG ACTGCCGCAC CCTGCGTCCG 
TCTCGGAAAC AAAAGCGACA GCAACAGCAG GTCCTGGAGC GGAAGAATCT ACCGTCCCAA 
AACCAGATCG AAAGCGAAGA CTCAGTGGAT GCTGGCGTCG ACGCCCGAAC ACGCTATCGC 
GTTATGAACA TGTCGCAGGC AGCTATCAAT GTTGAACTCG GAACAGCTTC CAATGAAATC 
GATCCGGATG TCAACTACAG AGAATCTCAC CAAGATCCCA TCGCTGATGC ACCTTCCCTC 
TCCACCCCAA GTGTAGCATC AACGGCGTCA GCCACGCCAT CGGTAACCCC AACACCGATT 
GCCACTCCGA CACCAACGGA GAGCACCAAG AACGACCAAG TGCTGTTTAA GAATTTCTTT 
GGCGCCACCA AGAATGCCAT ATTTCGCACA GCCCAGAGCA TTATTGAAAA CCATGAGAAG 
AAAAACGCTG CTAAGCAGAA GGAGCAGCCT GATCACTCAG GGACAGTCAG CTTAGTGGGT 
TCGCCAAATG GAGGAAGCAG TGTGCCACAA GACCTGGTCA AGTCTCCCAC AGACATTTCG 
AAAAAGAAGG AATTCTTTAG TTTGCTCACG TCTAGTTCCA GCATAAAGGC AGCGGCTGCA 
GCTGCGGCGA ACTCAGCTGC TAGCACTCCT CCTGCCACGC CTACGGAGGC ACTAGCTGCG 
AATTCAAATT CGAAGCTTCA ACCGCTTTCC GATCCATCTG CCGATTTAAA AGTAAAGGAA 
CGTACTCTCA ACTTGCGTCT ACCTGGAGAT AGTGAAGGCA GTGAAGGAGC ATTGACAAAG 
TGTTCATCAA TCAACTCGAT ACACAAACTA AAGCTTCCTG TTCCTGGTGT CGTTAAGTAT 
TTCATCAGTG AAAAAGCACC TGTGTCTGAT GTGCTCCAGA AGCCAGAGAA AGGCCAAAGC 
GGCCTTTTGC GTTTCTTTGA GTCGCCAGTG TTCAACATAC ACTTTGCTGT GCATTATCTC 
TTTTACTCAA AGGAACCGGG AGTCCTCAGC TTTATCGGCA ACAAGATCTT CAGTTTTCCC 
GACCAAGAGG TTGATCTGTA CATACCACAA TTGGTTGTTA TGTACATTCA GATGGATGAG 
CTAGCTGAGG TTCTGGATCC GTATCTTACC ATTCGGTGTC GCAAGTCGGT TGACTTCTCG 
CTGAAGTGTC TGTGGCTCTT GGAGGCATAT AACTACCAAG TGGACTCGCT AGGGAACTCA 
CACAACAGTT CTCGGAAATC AAAGCTGGCG CTCATGAAGG AAATATTCTC AAAAAGGGAG 
CACAAACAGA CTCAAAACGA CCTCAAGTCT GCAGGTACTG GAGGTCTGCA TGTTGGAGAG 
CCGAGGTCGG TAGTCGCGCT TGCCAAGAAG ACTCACCACC GCTCGCAATC TGATGCCACT 
GTGCTCCTAG CCGACTTTCG CTCGCCTCAC ACACTGAGTA TATCGCATCG TATGTACCAG 
CAGCCACCCT ATACCACACA AACACTTCCC ACTACGCCCG CCAAGCTGTG CCTCGGTGAC 
TTAACTTCCG GCCACGCATT TGATAACGGG TGCACCTGCT TCGAGACGGT TCGAGGCCAA 
GTAAATGGTC TGCTTGGGCA GAGGACGCTA TGCAGCTGTG GTGCTCCAAA GACGTCACCC 
CAGAAAGAGT TTATGAAGGC ATTGATGAAC GTTGGTAAAA ATCTCACCTC GTGGCCTTCC 
AAAGCGGAGA AGACTTCAGC GCTGCGGATG TCCCTCAACT TGATTAACAA AAACCTGCCC 
GCTAGAGTCT GGCTTCCTTT GTACTCAGAC ATTCCACATC ATGTCGTTCG TATTACAGAG 
GAGAAGACCG CAGTACTGAA CTCGAAGGAT AAAACCCCCT ATATCATATA CGTAGAAGTT 
GTTGAAATTC CTGACATCTA TACTTCTCCC CTGATTCCCA AAATGATGCC TTCACTGCGG 
CACACCAAGA GCGAAGAGCA CCTGGATGGG TCCTGCCTGA ACTCCCACCA ACATCTGAGC 
TGCAGTCGAA CTTCAAGCTG CAGTAGCAGT CAGCAAGGCA CAAGTTGCCG CCGAAAAAAA 
GGAGCAGATG TAGATGGCGG CTGCGGGGAT GCGGGTCCAC ATAATAGCTG CAGCAATGTA 
CATTCGTTAG GTGGACTTCA GTTCCAGGAA GATGACGTGT GGTCACAAGA GGAGGACGAA 
ATAACAGCGC AGTATCTTAA CATGCGGAAA GTGAGCGAGC GGGACACAAT TTCACAAATA 
TCACTAGATT CCTGCGATTC AAGAGATCAA GGTCCACCAG TCGTGTTTAA TATTGGCGAC 
GTGCGCTTGC GCCACTGCAG CAATTTGAGC TGCGAAAATA CTAAATCCTT CAGTAACGAC 
CCGGAGGATC CTTCGGCGGC AGCTTTAAAG GAACCGTGGC ACGAAAAGGA AAAGCTTATC 
CGCGAGTCTT CGCCATACGG CCATCTATCA AACTGGCGAC TGCTTTCCGC CATCGTAAAG 
TGTGGCGATG ATTTACGCCA AGAACTAATG GCTACCCAAC TTTTACAGAT GTTTAAAATT 
ATTTGGCAAG AAGAGCAAGT AGATCTGTGG GTGCGCCCGT ACAAAATAGT CTGCCTCTCG 
AATGATAGTG GTCTTATAGA GCCGATCCTT AACACTGTAT CCCTGCACCA AATTAAGAAG 
AACTCCAACA AATCGTTGAA AGAGTATTTT ATCGATGAAT ACGGCTCACC AAGCGGAGAA 
AGTTTTCGGC GCGCGCAGAA GAACTTCGTC CAAAGCTGTG CAGCTTACTG TCTAATTTCA 
TATTTACTGC AAGTTAAGGA CAGGCATAAT GGCAACATTC TCTTCCATTC GGATGGGCAC 
ATTATACACA TTGACTTTGG ATTTATTTTA AGTATATCAC CTAAAAACTT GGGATTCGAA 
CAGTCGCCCT TTAAGCTAAC GCCGGAATTT GTTGAGGTGA TGGGTGGCAC GAGCTCGGAG 
CATTGGCGTG AATTTAATAA GCTCTTGTTA GTTGGAATGA TGTCTGCTCG AAAGCATATG 
GATCGTATTA TTAACTTCGT GGAAATTATG CGCAGCAATG CGCATTTGCC GTGCTTTAAG 
AATGGGTGCT CTGGAACCGT ACAGAATCTC CGAAAACGCT TTCATATGAA TTTAACCGAA 
CAAGAAATGG AGCGAAAGGT TGAGCAGCTT GTACAGGACT CGTTAAAAAG TCTGTCAACC 
AAACTGTACG ACGGTTATCA ATACTACACT AATGGCATAT TGTGAGAACG GTTTTTTATG 
AAGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356971.1
CDS	1185..6233
Translation	MNSVISAFKGKKNSNPSCNSPMGSANGTAPVEAKPNNCYQYLRHIREIDTNNKLLSPVVVVAEERPVSKAGSPSTVATMATGVDVVDHCLPTTVRLSVTEALSDLNMNGKAVEPPVTPCTSVGDNANLEVAETWACPPPQATDDEDISEHSTAACTSTSSSSEHSNSTVVHSPTVGESTCDSVSVSVSHPSPSVSQNNTSSATGRMSSPHSQSSLSIACEPHSASTTTLSSLGSDLGNGNGSTVPAFLMAAPVTLPIGGQMPTIKCLPGASSAASTPKHSRYSKPRLSLSRVFNHSMPSVHGRPNLSLAPAGVPGSVNPPKRISTHQRNLSLDFRSMGILLPPVSQVTNNHINHTQHHRNRSLDSALQRIPEVEVSSPNAESENTFCSPSILGATMCSKIATTAATVTSALSPPGTPTPALMSTACTPTIGAVKGNVQHPVPKHEQSISLAAACRSSAVVESCPTLRMRPKSSESVAANGQEMIGVIVSSTGNGSSSSNNSCSSAGSTKKREDLTSLGSDDSGIICGSESDQISLNRICHSHESLDSGEMDADADAEEECVDLMDTTSIDEEYNIMQPDHLCLYPPQAASTELDCRTLRPSRKQKRQQQQVLERKNLPSQNQIESEDSVDAGVDARTRYRVMNMSQAAINVELGTASNEIDPDVNYRESHQDPIADAPSLSTPSVASTASATPSVTPTPIATPTPTESTKNDQVLFKNFFGATKNAIFRTAQSIIENHEKKNAAKQKEQPDHSGTVSLVGSPNGGSSVPQDLVKSPTDISKKKEFFSLLTSSSSIKAAAAAAANSAASTPPATPTEALAANSNSKLQPLSDPSADLKVKERTLNLRLPGDSEGSEGALTKCSSINSIHKLKLPVPGVVKYFISEKAPVSDVLQKPEKGQSGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFSFPDQEVDLYIPQLVVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNSHNSSRKSKLALMKEIFSKREHKQTQNDLKSAGTGGLHVGEPRSVVALAKKTHHRSQSDATVLLADFRSPHTLSISHRMYQQPPYTTQTLPTTPAKLCLGDLTSGHAFDNGCTCFETVRGQVNGLLGQRTLCSCGAPKTSPQKEFMKALMNVGKNLTSWPSKAEKTSALRMSLNLINKNLPARVWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPKMMPSLRHTKSEEHLDGSCLNSHQHLSCSRTSSCSSSQQGTSCRRKKGADVDGGCGDAGPHNSCSNVHSLGGLQFQEDDVWSQEEDEITAQYLNMRKVSERDTISQISLDSCDSRDQGPPVVFNIGDVRLRHCSNLSCENTKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKKNSNKSLKEYFIDEYGSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISPKNLGFEQSPFKLTPEFVEVMGGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRSNAHLPCFKNGCSGTVQNLRKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGIL*ERFFMKI

Target ORF information:

RefSeq Version	NM_001370043.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster four wheel drive (fwd), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370043.1

ORF Insert Sequence:

ATGAACTCCG TAATATCAGC ATTTAAAGGA AAAAAAAATA GCAATCCATC CTGCAATTCT 
CCAATGGGAT CGGCGAATGG CACTGCTCCG GTGGAGGCAA AGCCAAACAA TTGCTACCAG 
TACCTGCGCC ACATACGCGA AATCGATACA AACAACAAGC TCCTTTCTCC CGTCGTGGTT 
GTTGCAGAGG AGAGGCCAGT GAGCAAGGCT GGTTCGCCCT CGACAGTGGC GACCATGGCT 
ACTGGAGTCG ATGTGGTGGA TCACTGCCTG CCCACAACGG TACGATTGTC CGTTACCGAG 
GCGCTATCCG ACTTGAACAT GAACGGAAAA GCCGTCGAAC CCCCCGTCAC CCCATGCACT 
TCGGTTGGCG ACAATGCCAA TCTGGAGGTG GCTGAAACAT GGGCTTGTCC ACCGCCACAA 
GCGACGGATG ACGAGGACAT ATCCGAGCAC TCGACAGCCG CCTGCACCAG CACAAGTAGC 
TCCAGCGAGC ACAGCAACAG CACCGTGGTG CACAGTCCAA CTGTGGGGGA GAGCACCTGT 
GACTCCGTGT CCGTCTCGGT AAGCCATCCA AGTCCCAGCG TGAGTCAGAA CAACACATCG 
TCCGCCACCG GCCGGATGAG TAGCCCCCAC AGCCAGTCGT CGCTCTCCAT AGCCTGCGAG 
CCGCACTCGG CGTCGACCAC CACGCTGTCG TCGCTGGGAT CAGACCTGGG CAATGGAAAC 
GGAAGTACCG TACCCGCGTT TCTAATGGCC GCCCCCGTCA CGCTGCCAAT TGGCGGCCAG 
ATGCCCACGA TAAAGTGCCT CCCAGGAGCA AGCTCAGCGG CCAGCACGCC CAAGCACTCG 
CGCTACTCTA AGCCTCGTCT CAGCCTGAGC CGCGTGTTTA ATCACTCTAT GCCCTCCGTG 
CACGGCCGAC CAAATCTCAG TTTAGCGCCA GCTGGAGTTC CGGGAAGTGT GAATCCGCCG 
AAACGTATTT CCACGCACCA AAGAAACCTA AGCTTGGATT TTAGATCCAT GGGCATACTT 
CTTCCGCCTG TCTCGCAGGT GACCAATAAT CACATCAATC ACACCCAGCA CCACCGCAAT 
CGCAGCCTCG ACAGTGCCTT GCAACGCATT CCGGAGGTCG AAGTGTCATC GCCAAATGCT 
GAAAGCGAAA ACACGTTCTG CTCACCGTCC ATTCTCGGTG CGACTATGTG CTCAAAAATT 
GCCACGACAG CGGCAACTGT AACGTCAGCC CTGTCCCCAC CAGGCACGCC CACACCGGCT 
CTGATGTCGA CGGCGTGCAC GCCCACGATT GGAGCCGTAA AGGGCAACGT GCAACATCCC 
GTGCCGAAGC ATGAGCAATC GATTTCTTTG GCAGCTGCTT GCAGGTCCAG TGCCGTCGTG 
GAATCCTGTC CAACGTTACG AATGCGCCCA AAATCCAGCG AGTCGGTTGC GGCCAATGGA 
CAAGAGATGA TCGGCGTCAT TGTCAGCAGC ACGGGCAACG GCAGTAGCAG CAGTAATAAT 
AGCTGTAGTA GCGCTGGTAG CACCAAGAAG CGAGAGGATC TCACCAGTCT TGGATCGGAT 
GATTCTGGCA TCATCTGTGG CTCAGAGTCG GACCAAATAT CTCTGAATCG CATATGCCAC 
TCACATGAGT CGCTTGATTC GGGTGAGATG GATGCGGATG CCGATGCAGA AGAGGAGTGC 
GTTGATCTTA TGGATACGAC ATCCATCGAC GAGGAGTACA ACATTATGCA GCCGGACCAT 
CTCTGCCTCT ATCCTCCACA GGCCGCTTCC ACCGAATTGG ACTGCCGCAC CCTGCGTCCG 
TCTCGGAAAC AAAAGCGACA GCAACAGCAG GTCCTGGAGC GGAAGAATCT ACCGTCCCAA 
AACCAGATCG AAAGCGAAGA CTCAGTGGAT GCTGGCGTCG ACGCCCGAAC ACGCTATCGC 
GTTATGAACA TGTCGCAGGC AGCTATCAAT GTTGAACTCG GAACAGCTTC CAATGAAATC 
GATCCGGATG TCAACTACAG AGAATCTCAC CAAGATCCCA TCGCTGATGC ACCTTCCCTC 
TCCACCCCAA GTGTAGCATC AACGGCGTCA GCCACGCCAT CGGTAACCCC AACACCGATT 
GCCACTCCGA CACCAACGGA GAGCACCAAG AACGACCAAG TGCTGTTTAA GAATTTCTTT 
GGCGCCACCA AGAATGCCAT ATTTCGCACA GCCCAGAGCA TTATTGAAAA CCATGAGAAG 
AAAAACGCTG CTAAGCAGAA GGAGCAGCCT GATCACTCAG GGACAGTCAG CTTAGTGGGT 
TCGCCAAATG GAGGAAGCAG TGTGCCACAA GACCTGGTCA AGTCTCCCAC AGACATTTCG 
AAAAAGAAGG AATTCTTTAG TTTGCTCACG TCTAGTTCCA GCATAAAGGC AGCGGCTGCA 
GCTGCGGCGA ACTCAGCTGC TAGCACTCCT CCTGCCACGC CTACGGAGGC ACTAGCTGCG 
AATTCAAATT CGAAGCTTCA ACCGCTTTCC GATCCATCTG CCGATTTAAA AGTAAAGGAA 
CGTACTCTCA ACTTGCGTCT ACCTGGAGAT AGTGAAGGCA GTGAAGGAGC ATTGACAAAG 
TGTTCATCAA TCAACTCGAT ACACAAACTA AAGCTTCCTG TTCCTGGTGT CGTTAAGTAT 
TTCATCAGTG AAAAAGCACC TGTGTCTGAT GTGCTCCAGA AGCCAGAGAA AGGCCAAAGC 
GGCCTTTTGC GTTTCTTTGA GTCGCCAGTG TTCAACATAC ACTTTGCTGT GCATTATCTC 
TTTTACTCAA AGGAACCGGG AGTCCTCAGC TTTATCGGCA ACAAGATCTT CAGTTTTCCC 
GACCAAGAGG TTGATCTGTA CATACCACAA TTGGTTGTTA TGTACATTCA GATGGATGAG 
CTAGCTGAGG TTCTGGATCC GTATCTTACC ATTCGGTGTC GCAAGTCGGT TGACTTCTCG 
CTGAAGTGTC TGTGGCTCTT GGAGGCATAT AACTACCAAG TGGACTCGCT AGGGAACTCA 
CACAACAGTT CTCGGAAATC AAAGCTGGCG CTCATGAAGG AAATATTCTC AAAAAGGGAG 
CACAAACAGA CTCAAAACGA CCTCAAGTCT GCAGGTACTG GAGGTCTGCA TGTTGGAGAG 
CCGAGGTCGG TAGTCGCGCT TGCCAAGAAG ACTCACCACC GCTCGCAATC TGATGCCACT 
GTGCTCCTAG CCGACTTTCG CTCGCCTCAC ACACTGAGTA TATCGCATCG TATGTACCAG 
CAGCCACCCT ATACCACACA AACACTTCCC ACTACGCCCG CCAAGCTGTG CCTCGGTGAC 
TTAACTTCCG GCCACGCATT TGATAACGGG TGCACCTGCT TCGAGACGGT TCGAGGCCAA 
GTAAATGGTC TGCTTGGGCA GAGGACGCTA TGCAGCTGTG GTGCTCCAAA GACGTCACCC 
CAGAAAGAGT TTATGAAGGC ATTGATGAAC GTTGGTAAAA ATCTCACCTC GTGGCCTTCC 
AAAGCGGAGA AGACTTCAGC GCTGCGGATG TCCCTCAACT TGATTAACAA AAACCTGCCC 
GCTAGAGTCT GGCTTCCTTT GTACTCAGAC ATTCCACATC ATGTCGTTCG TATTACAGAG 
GAGAAGACCG CAGTACTGAA CTCGAAGGAT AAAACCCCCT ATATCATATA CGTAGAAGTT 
GTTGAAATTC CTGACATCTA TACTTCTCCC CTGATTCCCA AAATGATGCC TTCACTGCGG 
CACACCAAGA GCGAAGAGCA CCTGGATGGG TCCTGCCTGA ACTCCCACCA ACATCTGAGC 
TGCAGTCGAA CTTCAAGCTG CAGTAGCAGT CAGCAAGGCA CAAGTTGCCG CCGAAAAAAA 
GGAGCAGATG TAGATGGCGG CTGCGGGGAT GCGGGTCCAC ATAATAGCTG CAGCAATGTA 
CATTCGTTAG GTGGACTTCA GTTCCAGGAA GATGACGTGT GGTCACAAGA GGAGGACGAA 
ATAACAGCGC AGTATCTTAA CATGCGGAAA GTGAGCGAGC GGGACACAAT TTCACAAATA 
TCACTAGATT CCTGCGATTC AAGAGATCAA GGTCCACCAG TCGTGTTTAA TATTGGCGAC 
GTGCGCTTGC GCCACTGCAG CAATTTGAGC TGCGAAAATA CTAAATCCTT CAGTAACGAC 
CCGGAGGATC CTTCGGCGGC AGCTTTAAAG GAACCGTGGC ACGAAAAGGA AAAGCTTATC 
CGCGAGTCTT CGCCATACGG CCATCTATCA AACTGGCGAC TGCTTTCCGC CATCGTAAAG 
TGTGGCGATG ATTTACGCCA AGAACTAATG GCTACCCAAC TTTTACAGAT GTTTAAAATT 
ATTTGGCAAG AAGAGCAAGT AGATCTGTGG GTGCGCCCGT ACAAAATAGT CTGCCTCTCG 
AATGATAGTG GTCTTATAGA GCCGATCCTT AACACTGTAT CCCTGCACCA AATTAAGAAG 
AACTCCAACA AATCGTTGAA AGAGTATTTT ATCGATGAAT ACGGCTCACC AAGCGGAGAA 
AGTTTTCGGC GCGCGCAGAA GAACTTCGTC CAAAGCTGTG CAGCTTACTG TCTAATTTCA 
TATTTACTGC AAGTTAAGGA CAGGCATAAT GGCAACATTC TCTTCCATTC GGATGGGCAC 
ATTATACACA TTGACTTTGG ATTTATTTTA AGTATATCAC CTAAAAACTT GGGATTCGAA 
CAGTCGCCCT TTAAGCTAAC GCCGGAATTT GTTGAGGTGA TGGGTGGCAC GAGCTCGGAG 
CATTGGCGTG AATTTAATAA GCTCTTGTTA GTTGGAATGA TGTCTGCTCG AAAGCATATG 
GATCGTATTA TTAACTTCGT GGAAATTATG CGCAGCAATG CGCATTTGCC GTGCTTTAAG 
AATGGGTGCT CTGGAACCGT ACAGAATCTC CGAAAACGCT TTCATATGAA TTTAACCGAA 
CAAGAAATGG AGCGAAAGGT TGAGCAGCTT GTACAGGACT CGTTAAAAAG TCTGTCAACC 
AAACTGTACG ACGGTTATCA ATACTACACT AATGGCATAT TGTGAGAACG GTTTTTTATG 
AAGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fwd ( NM_167826.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728518.1 Drosophila melanogaster four wheel drive (fwd), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19402
Clone ID Related Accession (Same CDS sequence)	NM_167826.2 , NM_001274274.1 , NM_080083.4
Accession Version	NM_167826.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	four wheel drive, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167826.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCCG TAATATCAGC ATTTAAAGGA AAAAAAAATA GCAATCCATC CTGCAATTCT 
CCAATGGGAT CGGCGAATGG CACTGCTCCG GTGGAGGCAA AGCCAAACAA TTGCTACCAG 
TACCTGCGCC ACATACGCGA AATCGATACA AACAACAAGC TCCTTTCTCC CGTCGTGGTT 
GTTGCAGAGG AGAGGCCAGT GAGCAAGGCT GGTTCGCCCT CGACAGTGGC GACCATGGCT 
ACTGGAGTCG ATGTGGTGGA TCACTGCCTG CCCACAACGG TACGATTGTC CGTTACCGAG 
GCGCTATCCG ACTTGAACAT GAACGGAAAA GCCGTCGAAC CCCCCGTCAC CCCATGCACT 
TCGGTTGGCG ACAATGCCAA TCTGGAGGTG GCTGAAACAT GGGCTTGTCC ACCGCCACAA 
GCGACGGATG ACGAGGACAT ATCCGAGCAC TCGACAGCCG CCTGCACCAG CACAAGTAGC 
TCCAGCGAGC ACAGCAACAG CACCGTGGTG CACAGTCCAA CTGTGGGGGA GAGCACCTGT 
GACTCCGTGT CCGTCTCGGT AAGCCATCCA AGTCCCAGCG TGAGTCAGAA CAACACATCG 
TCCGCCACCG GCCGGATGAG TAGCCCCCAC AGCCAGTCGT CGCTCTCCAT AGCCTGCGAG 
CCGCACTCGG CGTCGACCAC CACGCTGTCG TCGCTGGGAT CAGACCTGGG CAATGGAAAC 
GGAAGTACCG TACCCGCGTT TCTAATGGCC GCCCCCGTCA CGCTGCCAAT TGGCGGCCAG 
ATGCCCACGA TAAAGTGCCT CCCAGGAGCA AGCTCAGCGG CCAGCACGCC CAAGCACTCG 
CGCTACTCTA AGCCTCGTCT CAGCCTGAGC CGCGTGTTTA ATCACTCTAT GCCCTCCGTG 
CACGGCCGAC CAAATCTCAG TTTAGCGCCA GCTGGAGTTC CGGGAAGTGT GAATCCGCCG 
AAACGTATTT CCACGCACCA AAGAAACCTA AGCTTGGATT TTAGATCCAT GGGCATACTT 
CTTCCGCCTG TCTCGCAGGT GACCAATAAT CACATCAATC ACACCCAGCA CCACCGCAAT 
CGCAGCCTCG ACAGTGCCTT GCAACGCATT CCGGAGGTCG AAGTGTCATC GCCAAATGCT 
GAAAGCGAAA ACACGTTCTG CTCACCGTCC ATTCTCGGTG CGACTATGTG CTCAAAAATT 
GCCACGACAG CGGCAACTGT AACGTCAGCC CTGTCCCCAC CAGGCACGCC CACACCGGCT 
CTGATGTCGA CGGCGTGCAC GCCCACGATT GGAGCCGTAA AGGGCAACGT GCAACATCCC 
GTGCCGAAGC ATGAGCAATC GATTTCTTTG GCAGCTGCTT GCAGGTCCAG TGCCGTCGTG 
GAATCCTGTC CAACGTTACG AATGCGCCCA AAATCCAGCG AGTCGGTTGC GGCCAATGGA 
CAAGAGATGA TCGGCGTCAT TGTCAGCAGC ACGGGCAACG GCAGTAGCAG CAGTAATAAT 
AGCTGTAGTA GCGCTGGTAG CACCAAGAAG CGAGAGGATC TCACCAGTCT TGGATCGGAT 
GATTCTGGCA TCATCTGTGG CTCAGAGTCG GACCAAATAT CTCTGAATCG CATATGCCAC 
TCACATGAGT CGCTTGATTC GGGTGAGATG GATGCGGATG CCGATGCAGA AGAGGAGTGC 
GTTGATCTTA TGGATACGAC ATCCATCGAC GAGGAGTACA ACATTATGCA GCCGGACCAT 
CTCTGCCTCT ATCCTCCACA GGCCGCTTCC ACCGAATTGG ACTGCCGCAC CCTGCGTCCG 
TCTCGGAAAC AAAAGCGACA GCAACAGCAG GTCCTGGAGC GGAAGAATCT ACCGTCCCAA 
AACCAGATCG AAAGCGAAGA CTCAGTGGAT GCTGGCGTCG ACGCCCGAAC ACGCTATCGC 
GTTATGAACA TGTCGCAGGC AGCTATCAAT GTTGAACTCG GAACAGCTTC CAATGAAATC 
GATCCGGATG TCAACTACAG AGAATCTCAC CAAGATCCCA TCGCTGATGC ACCTTCCCTC 
TCCACCCCAA GTGTAGCATC AACGGCGTCA GCCACGCCAT CGGTAACCCC AACACCGATT 
GCCACTCCGA CACCAACGGA GAGCACCAAG AACGACCAAG TGCTGTTTAA GAATTTCTTT 
GGCGCCACCA AGAATGCCAT ATTTCGCACA GCCCAGAGCA TTATTGAAAA CCATGAGAAG 
AAAAACGCTG CTAAGCAGAA GGAGCAGCCT GATCACTCAG GGACAGTCAG CTTAGTGGGT 
TCGCCAAATG GAGGAAGCAG TGTGCCACAA GACCTGGTCA AGTCTCCCAC AGACATTTCG 
AAAAAGAAGG AATTCTTTAG TTTGCTCACG TCTAGTTCCA GCATAAAGGC AGCGGCTGCA 
GCTGCGGCGA ACTCAGCTGC TAGCACTCCT CCTGCCACGC CTACGGAGGC ACTAGCTGCG 
AATTCAAATT CGAAGCTTCA ACCGCTTTCC GATCCATCTG CCGATTTAAA AGTAAAGGAA 
CGTACTCTCA ACTTGCGTCT ACCTGGAGAT AGTGAAGGCA GTGAAGGAGC ATTGACAAAG 
TGTTCATCAA TCAACTCGAT ACACAAACTA AAGCTTCCTG TTCCTGGTGT CGTTAAGTAT 
TTCATCAGTG AAAAAGCACC TGTGTCTGAT GTGCTCCAGA AGCCAGAGAA AGGCCAAAGC 
GGCCTTTTGC GTTTCTTTGA GTCGCCAGTG TTCAACATAC ACTTTGCTGT GCATTATCTC 
TTTTACTCAA AGGAACCGGG AGTCCTCAGC TTTATCGGCA ACAAGATCTT CAGTTTTCCC 
GACCAAGAGG TTGATCTGTA CATACCACAA TTGGTTGTTA TGTACATTCA GATGGATGAG 
CTAGCTGAGG TTCTGGATCC GTATCTTACC ATTCGGTGTC GCAAGTCGGT TGACTTCTCG 
CTGAAGTGTC TGTGGCTCTT GGAGGCATAT AACTACCAAG TGGACTCGCT AGGGAACTCA 
CACAACAGTT CTCGGAAATC AAAGCTGGCG CTCATGAAGG AAATATTCTC AAAAAGGGAG 
CACAAACAGA CTCAAAACGA CCTCAAGTCT GCAGGTACTG GAGGTCTGCA TGTTGGAGAG 
CCGAGGTCGG TAGTCGCGCT TGCCAAGAAG ACTCACCACC GCTCGCAATC TGATGCCACT 
GTGCTCCTAG CCGACTTTCG CTCGCCTCAC ACACTGAGTA TATCGCATCG TATGTACCAG 
CAGCCACCCT ATACCACACA AACACTTCCC ACTACGCCCG CCAAGCTGTG CCTCGGTGAC 
TTAACTTCCG GCCACGCATT TGATAACGGG TGCACCTGCT TCGAGACGGT TCGAGGCCAA 
GTAAATGGTC TGCTTGGGCA GAGGACGCTA TGCAGCTGTG GTGCTCCAAA GACGTCACCC 
CAGAAAGAGT TTATGAAGGC ATTGATGAAC GTTGGTAAAA ATCTCACCTC GTGGCCTTCC 
AAAGCGGAGA AGACTTCAGC GCTGCGGATG TCCCTCAACT TGATTAACAA AAACCTGCCC 
GCTAGAGTCT GGCTTCCTTT GTACTCAGAC ATTCCACATC ATGTCGTTCG TATTACAGAG 
GAGAAGACCG CAGTACTGAA CTCGAAGGAT AAAACCCCCT ATATCATATA CGTAGAAGTT 
GTTGAAATTC CTGACATCTA TACTTCTCCC CTGATTCCCA AAATGATGCC TTCACTGCGG 
CACACCAAGA GCGAAGAGCA CCTGGATGGG TCCTGCCTGA ACTCCCACCA ACATCTGAGC 
TGCAGTCGAA CTTCAAGCTG CAGTAGCAGT CAGCAAGGCA CAAGTTGCCG CCGAAAAAAA 
GGAGCAGATG TAGATGGCGG CTGCGGGGAT GCGGGTCCAC ATAATAGCTG CAGCAATGTA 
CATTCGTTAG GTGGACTTCA GTTCCAGGAA GATGACGTGT GGTCACAAGA GGAGGACGAA 
ATAACAGCGC AGTATCTTAA CATGCGGAAA GTGAGCGAGC GGGACACAAT TTCACAAATA 
TCACTAGATT CCTGCGATTC AAGAGATCAA GGTCCACCAG TCGTGTTTAA TATTGGCGAC 
GTGCGCTTGC GCCACTGCAG CAATTTGAGC TGCGAAAATA CTAAATCCTT CAGTAACGAC 
CCGGAGGATC CTTCGGCGGC AGCTTTAAAG GAACCGTGGC ACGAAAAGGA AAAGCTTATC 
CGCGAGTCTT CGCCATACGG CCATCTATCA AACTGGCGAC TGCTTTCCGC CATCGTAAAG 
TGTGGCGATG ATTTACGCCA AGAACTAATG GCTACCCAAC TTTTACAGAT GTTTAAAATT 
ATTTGGCAAG AAGAGCAAGT AGATCTGTGG GTGCGCCCGT ACAAAATAGT CTGCCTCTCG 
AATGATAGTG GTCTTATAGA GCCGATCCTT AACACTGTAT CCCTGCACCA AATTAAGAAG 
AACTCCAACA AATCGTTGAA AGAGTATTTT ATCGATGAAT ACGGCTCACC AAGCGGAGAA 
AGTTTTCGGC GCGCGCAGAA GAACTTCGTC CAAAGCTGTG CAGCTTACTG TCTAATTTCA 
TATTTACTGC AAGTTAAGGA CAGGCATAAT GGCAACATTC TCTTCCATTC GGATGGGCAC 
ATTATACACA TTGACTTTGG ATTTATTTTA AGTATATCAC CTAAAAACTT GGGATTCGAA 
CAGTCGCCCT TTAAGCTAAC GCCGGAATTT GTTGAGGTGA TGGGTGGCAC GAGCTCGGAG 
CATTGGCGTG AATTTAATAA GCTCTTGTTA GTTGGAATGA TGTCTGCTCG AAAGCATATG 
GATCGTATTA TTAACTTCGT GGAAATTATG CGCAGCAATG CGCATTTGCC GTGCTTTAAG 
AATGGGTGCT CTGGAACCGT ACAGAATCTC CGAAAACGCT TTCATATGAA TTTAACCGAA 
CAAGAAATGG AGCGAAAGGT TGAGCAGCTT GTACAGGACT CGTTAAAAAG TCTGTCAACC 
AAACTGTACG ACGGTTATCA ATACTACACT AATGGCATAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728518.1
CDS	429..5453
Translation	MNSVISAFKGKKNSNPSCNSPMGSANGTAPVEAKPNNCYQYLRHIREIDTNNKLLSPVVVVAEERPVSKAGSPSTVATMATGVDVVDHCLPTTVRLSVTEALSDLNMNGKAVEPPVTPCTSVGDNANLEVAETWACPPPQATDDEDISEHSTAACTSTSSSSEHSNSTVVHSPTVGESTCDSVSVSVSHPSPSVSQNNTSSATGRMSSPHSQSSLSIACEPHSASTTTLSSLGSDLGNGNGSTVPAFLMAAPVTLPIGGQMPTIKCLPGASSAASTPKHSRYSKPRLSLSRVFNHSMPSVHGRPNLSLAPAGVPGSVNPPKRISTHQRNLSLDFRSMGILLPPVSQVTNNHINHTQHHRNRSLDSALQRIPEVEVSSPNAESENTFCSPSILGATMCSKIATTAATVTSALSPPGTPTPALMSTACTPTIGAVKGNVQHPVPKHEQSISLAAACRSSAVVESCPTLRMRPKSSESVAANGQEMIGVIVSSTGNGSSSSNNSCSSAGSTKKREDLTSLGSDDSGIICGSESDQISLNRICHSHESLDSGEMDADADAEEECVDLMDTTSIDEEYNIMQPDHLCLYPPQAASTELDCRTLRPSRKQKRQQQQVLERKNLPSQNQIESEDSVDAGVDARTRYRVMNMSQAAINVELGTASNEIDPDVNYRESHQDPIADAPSLSTPSVASTASATPSVTPTPIATPTPTESTKNDQVLFKNFFGATKNAIFRTAQSIIENHEKKNAAKQKEQPDHSGTVSLVGSPNGGSSVPQDLVKSPTDISKKKEFFSLLTSSSSIKAAAAAAANSAASTPPATPTEALAANSNSKLQPLSDPSADLKVKERTLNLRLPGDSEGSEGALTKCSSINSIHKLKLPVPGVVKYFISEKAPVSDVLQKPEKGQSGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFSFPDQEVDLYIPQLVVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNSHNSSRKSKLALMKEIFSKREHKQTQNDLKSAGTGGLHVGEPRSVVALAKKTHHRSQSDATVLLADFRSPHTLSISHRMYQQPPYTTQTLPTTPAKLCLGDLTSGHAFDNGCTCFETVRGQVNGLLGQRTLCSCGAPKTSPQKEFMKALMNVGKNLTSWPSKAEKTSALRMSLNLINKNLPARVWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPKMMPSLRHTKSEEHLDGSCLNSHQHLSCSRTSSCSSSQQGTSCRRKKGADVDGGCGDAGPHNSCSNVHSLGGLQFQEDDVWSQEEDEITAQYLNMRKVSERDTISQISLDSCDSRDQGPPVVFNIGDVRLRHCSNLSCENTKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKKNSNKSLKEYFIDEYGSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISPKNLGFEQSPFKLTPEFVEVMGGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRSNAHLPCFKNGCSGTVQNLRKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGIL

Target ORF information:

RefSeq Version	NM_167826.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster four wheel drive (fwd), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167826.2

ORF Insert Sequence:

ATGAACTCCG TAATATCAGC ATTTAAAGGA AAAAAAAATA GCAATCCATC CTGCAATTCT 
CCAATGGGAT CGGCGAATGG CACTGCTCCG GTGGAGGCAA AGCCAAACAA TTGCTACCAG 
TACCTGCGCC ACATACGCGA AATCGATACA AACAACAAGC TCCTTTCTCC CGTCGTGGTT 
GTTGCAGAGG AGAGGCCAGT GAGCAAGGCT GGTTCGCCCT CGACAGTGGC GACCATGGCT 
ACTGGAGTCG ATGTGGTGGA TCACTGCCTG CCCACAACGG TACGATTGTC CGTTACCGAG 
GCGCTATCCG ACTTGAACAT GAACGGAAAA GCCGTCGAAC CCCCCGTCAC CCCATGCACT 
TCGGTTGGCG ACAATGCCAA TCTGGAGGTG GCTGAAACAT GGGCTTGTCC ACCGCCACAA 
GCGACGGATG ACGAGGACAT ATCCGAGCAC TCGACAGCCG CCTGCACCAG CACAAGTAGC 
TCCAGCGAGC ACAGCAACAG CACCGTGGTG CACAGTCCAA CTGTGGGGGA GAGCACCTGT 
GACTCCGTGT CCGTCTCGGT AAGCCATCCA AGTCCCAGCG TGAGTCAGAA CAACACATCG 
TCCGCCACCG GCCGGATGAG TAGCCCCCAC AGCCAGTCGT CGCTCTCCAT AGCCTGCGAG 
CCGCACTCGG CGTCGACCAC CACGCTGTCG TCGCTGGGAT CAGACCTGGG CAATGGAAAC 
GGAAGTACCG TACCCGCGTT TCTAATGGCC GCCCCCGTCA CGCTGCCAAT TGGCGGCCAG 
ATGCCCACGA TAAAGTGCCT CCCAGGAGCA AGCTCAGCGG CCAGCACGCC CAAGCACTCG 
CGCTACTCTA AGCCTCGTCT CAGCCTGAGC CGCGTGTTTA ATCACTCTAT GCCCTCCGTG 
CACGGCCGAC CAAATCTCAG TTTAGCGCCA GCTGGAGTTC CGGGAAGTGT GAATCCGCCG 
AAACGTATTT CCACGCACCA AAGAAACCTA AGCTTGGATT TTAGATCCAT GGGCATACTT 
CTTCCGCCTG TCTCGCAGGT GACCAATAAT CACATCAATC ACACCCAGCA CCACCGCAAT 
CGCAGCCTCG ACAGTGCCTT GCAACGCATT CCGGAGGTCG AAGTGTCATC GCCAAATGCT 
GAAAGCGAAA ACACGTTCTG CTCACCGTCC ATTCTCGGTG CGACTATGTG CTCAAAAATT 
GCCACGACAG CGGCAACTGT AACGTCAGCC CTGTCCCCAC CAGGCACGCC CACACCGGCT 
CTGATGTCGA CGGCGTGCAC GCCCACGATT GGAGCCGTAA AGGGCAACGT GCAACATCCC 
GTGCCGAAGC ATGAGCAATC GATTTCTTTG GCAGCTGCTT GCAGGTCCAG TGCCGTCGTG 
GAATCCTGTC CAACGTTACG AATGCGCCCA AAATCCAGCG AGTCGGTTGC GGCCAATGGA 
CAAGAGATGA TCGGCGTCAT TGTCAGCAGC ACGGGCAACG GCAGTAGCAG CAGTAATAAT 
AGCTGTAGTA GCGCTGGTAG CACCAAGAAG CGAGAGGATC TCACCAGTCT TGGATCGGAT 
GATTCTGGCA TCATCTGTGG CTCAGAGTCG GACCAAATAT CTCTGAATCG CATATGCCAC 
TCACATGAGT CGCTTGATTC GGGTGAGATG GATGCGGATG CCGATGCAGA AGAGGAGTGC 
GTTGATCTTA TGGATACGAC ATCCATCGAC GAGGAGTACA ACATTATGCA GCCGGACCAT 
CTCTGCCTCT ATCCTCCACA GGCCGCTTCC ACCGAATTGG ACTGCCGCAC CCTGCGTCCG 
TCTCGGAAAC AAAAGCGACA GCAACAGCAG GTCCTGGAGC GGAAGAATCT ACCGTCCCAA 
AACCAGATCG AAAGCGAAGA CTCAGTGGAT GCTGGCGTCG ACGCCCGAAC ACGCTATCGC 
GTTATGAACA TGTCGCAGGC AGCTATCAAT GTTGAACTCG GAACAGCTTC CAATGAAATC 
GATCCGGATG TCAACTACAG AGAATCTCAC CAAGATCCCA TCGCTGATGC ACCTTCCCTC 
TCCACCCCAA GTGTAGCATC AACGGCGTCA GCCACGCCAT CGGTAACCCC AACACCGATT 
GCCACTCCGA CACCAACGGA GAGCACCAAG AACGACCAAG TGCTGTTTAA GAATTTCTTT 
GGCGCCACCA AGAATGCCAT ATTTCGCACA GCCCAGAGCA TTATTGAAAA CCATGAGAAG 
AAAAACGCTG CTAAGCAGAA GGAGCAGCCT GATCACTCAG GGACAGTCAG CTTAGTGGGT 
TCGCCAAATG GAGGAAGCAG TGTGCCACAA GACCTGGTCA AGTCTCCCAC AGACATTTCG 
AAAAAGAAGG AATTCTTTAG TTTGCTCACG TCTAGTTCCA GCATAAAGGC AGCGGCTGCA 
GCTGCGGCGA ACTCAGCTGC TAGCACTCCT CCTGCCACGC CTACGGAGGC ACTAGCTGCG 
AATTCAAATT CGAAGCTTCA ACCGCTTTCC GATCCATCTG CCGATTTAAA AGTAAAGGAA 
CGTACTCTCA ACTTGCGTCT ACCTGGAGAT AGTGAAGGCA GTGAAGGAGC ATTGACAAAG 
TGTTCATCAA TCAACTCGAT ACACAAACTA AAGCTTCCTG TTCCTGGTGT CGTTAAGTAT 
TTCATCAGTG AAAAAGCACC TGTGTCTGAT GTGCTCCAGA AGCCAGAGAA AGGCCAAAGC 
GGCCTTTTGC GTTTCTTTGA GTCGCCAGTG TTCAACATAC ACTTTGCTGT GCATTATCTC 
TTTTACTCAA AGGAACCGGG AGTCCTCAGC TTTATCGGCA ACAAGATCTT CAGTTTTCCC 
GACCAAGAGG TTGATCTGTA CATACCACAA TTGGTTGTTA TGTACATTCA GATGGATGAG 
CTAGCTGAGG TTCTGGATCC GTATCTTACC ATTCGGTGTC GCAAGTCGGT TGACTTCTCG 
CTGAAGTGTC TGTGGCTCTT GGAGGCATAT AACTACCAAG TGGACTCGCT AGGGAACTCA 
CACAACAGTT CTCGGAAATC AAAGCTGGCG CTCATGAAGG AAATATTCTC AAAAAGGGAG 
CACAAACAGA CTCAAAACGA CCTCAAGTCT GCAGGTACTG GAGGTCTGCA TGTTGGAGAG 
CCGAGGTCGG TAGTCGCGCT TGCCAAGAAG ACTCACCACC GCTCGCAATC TGATGCCACT 
GTGCTCCTAG CCGACTTTCG CTCGCCTCAC ACACTGAGTA TATCGCATCG TATGTACCAG 
CAGCCACCCT ATACCACACA AACACTTCCC ACTACGCCCG CCAAGCTGTG CCTCGGTGAC 
TTAACTTCCG GCCACGCATT TGATAACGGG TGCACCTGCT TCGAGACGGT TCGAGGCCAA 
GTAAATGGTC TGCTTGGGCA GAGGACGCTA TGCAGCTGTG GTGCTCCAAA GACGTCACCC 
CAGAAAGAGT TTATGAAGGC ATTGATGAAC GTTGGTAAAA ATCTCACCTC GTGGCCTTCC 
AAAGCGGAGA AGACTTCAGC GCTGCGGATG TCCCTCAACT TGATTAACAA AAACCTGCCC 
GCTAGAGTCT GGCTTCCTTT GTACTCAGAC ATTCCACATC ATGTCGTTCG TATTACAGAG 
GAGAAGACCG CAGTACTGAA CTCGAAGGAT AAAACCCCCT ATATCATATA CGTAGAAGTT 
GTTGAAATTC CTGACATCTA TACTTCTCCC CTGATTCCCA AAATGATGCC TTCACTGCGG 
CACACCAAGA GCGAAGAGCA CCTGGATGGG TCCTGCCTGA ACTCCCACCA ACATCTGAGC 
TGCAGTCGAA CTTCAAGCTG CAGTAGCAGT CAGCAAGGCA CAAGTTGCCG CCGAAAAAAA 
GGAGCAGATG TAGATGGCGG CTGCGGGGAT GCGGGTCCAC ATAATAGCTG CAGCAATGTA 
CATTCGTTAG GTGGACTTCA GTTCCAGGAA GATGACGTGT GGTCACAAGA GGAGGACGAA 
ATAACAGCGC AGTATCTTAA CATGCGGAAA GTGAGCGAGC GGGACACAAT TTCACAAATA 
TCACTAGATT CCTGCGATTC AAGAGATCAA GGTCCACCAG TCGTGTTTAA TATTGGCGAC 
GTGCGCTTGC GCCACTGCAG CAATTTGAGC TGCGAAAATA CTAAATCCTT CAGTAACGAC 
CCGGAGGATC CTTCGGCGGC AGCTTTAAAG GAACCGTGGC ACGAAAAGGA AAAGCTTATC 
CGCGAGTCTT CGCCATACGG CCATCTATCA AACTGGCGAC TGCTTTCCGC CATCGTAAAG 
TGTGGCGATG ATTTACGCCA AGAACTAATG GCTACCCAAC TTTTACAGAT GTTTAAAATT 
ATTTGGCAAG AAGAGCAAGT AGATCTGTGG GTGCGCCCGT ACAAAATAGT CTGCCTCTCG 
AATGATAGTG GTCTTATAGA GCCGATCCTT AACACTGTAT CCCTGCACCA AATTAAGAAG 
AACTCCAACA AATCGTTGAA AGAGTATTTT ATCGATGAAT ACGGCTCACC AAGCGGAGAA 
AGTTTTCGGC GCGCGCAGAA GAACTTCGTC CAAAGCTGTG CAGCTTACTG TCTAATTTCA 
TATTTACTGC AAGTTAAGGA CAGGCATAAT GGCAACATTC TCTTCCATTC GGATGGGCAC 
ATTATACACA TTGACTTTGG ATTTATTTTA AGTATATCAC CTAAAAACTT GGGATTCGAA 
CAGTCGCCCT TTAAGCTAAC GCCGGAATTT GTTGAGGTGA TGGGTGGCAC GAGCTCGGAG 
CATTGGCGTG AATTTAATAA GCTCTTGTTA GTTGGAATGA TGTCTGCTCG AAAGCATATG 
GATCGTATTA TTAACTTCGT GGAAATTATG CGCAGCAATG CGCATTTGCC GTGCTTTAAG 
AATGGGTGCT CTGGAACCGT ACAGAATCTC CGAAAACGCT TTCATATGAA TTTAACCGAA 
CAAGAAATGG AGCGAAAGGT TGAGCAGCTT GTACAGGACT CGTTAAAAAG TCTGTCAACC 
AAACTGTACG ACGGTTATCA ATACTACACT AATGGCATAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fwd ( NM_001274274.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261203.1 Drosophila melanogaster four wheel drive (fwd), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa19402
Clone ID Related Accession (Same CDS sequence)	NM_167826.2 , NM_001274274.1 , NM_080083.4
Accession Version	NM_001274274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	four wheel drive, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCCG TAATATCAGC ATTTAAAGGA AAAAAAAATA GCAATCCATC CTGCAATTCT 
CCAATGGGAT CGGCGAATGG CACTGCTCCG GTGGAGGCAA AGCCAAACAA TTGCTACCAG 
TACCTGCGCC ACATACGCGA AATCGATACA AACAACAAGC TCCTTTCTCC CGTCGTGGTT 
GTTGCAGAGG AGAGGCCAGT GAGCAAGGCT GGTTCGCCCT CGACAGTGGC GACCATGGCT 
ACTGGAGTCG ATGTGGTGGA TCACTGCCTG CCCACAACGG TACGATTGTC CGTTACCGAG 
GCGCTATCCG ACTTGAACAT GAACGGAAAA GCCGTCGAAC CCCCCGTCAC CCCATGCACT 
TCGGTTGGCG ACAATGCCAA TCTGGAGGTG GCTGAAACAT GGGCTTGTCC ACCGCCACAA 
GCGACGGATG ACGAGGACAT ATCCGAGCAC TCGACAGCCG CCTGCACCAG CACAAGTAGC 
TCCAGCGAGC ACAGCAACAG CACCGTGGTG CACAGTCCAA CTGTGGGGGA GAGCACCTGT 
GACTCCGTGT CCGTCTCGGT AAGCCATCCA AGTCCCAGCG TGAGTCAGAA CAACACATCG 
TCCGCCACCG GCCGGATGAG TAGCCCCCAC AGCCAGTCGT CGCTCTCCAT AGCCTGCGAG 
CCGCACTCGG CGTCGACCAC CACGCTGTCG TCGCTGGGAT CAGACCTGGG CAATGGAAAC 
GGAAGTACCG TACCCGCGTT TCTAATGGCC GCCCCCGTCA CGCTGCCAAT TGGCGGCCAG 
ATGCCCACGA TAAAGTGCCT CCCAGGAGCA AGCTCAGCGG CCAGCACGCC CAAGCACTCG 
CGCTACTCTA AGCCTCGTCT CAGCCTGAGC CGCGTGTTTA ATCACTCTAT GCCCTCCGTG 
CACGGCCGAC CAAATCTCAG TTTAGCGCCA GCTGGAGTTC CGGGAAGTGT GAATCCGCCG 
AAACGTATTT CCACGCACCA AAGAAACCTA AGCTTGGATT TTAGATCCAT GGGCATACTT 
CTTCCGCCTG TCTCGCAGGT GACCAATAAT CACATCAATC ACACCCAGCA CCACCGCAAT 
CGCAGCCTCG ACAGTGCCTT GCAACGCATT CCGGAGGTCG AAGTGTCATC GCCAAATGCT 
GAAAGCGAAA ACACGTTCTG CTCACCGTCC ATTCTCGGTG CGACTATGTG CTCAAAAATT 
GCCACGACAG CGGCAACTGT AACGTCAGCC CTGTCCCCAC CAGGCACGCC CACACCGGCT 
CTGATGTCGA CGGCGTGCAC GCCCACGATT GGAGCCGTAA AGGGCAACGT GCAACATCCC 
GTGCCGAAGC ATGAGCAATC GATTTCTTTG GCAGCTGCTT GCAGGTCCAG TGCCGTCGTG 
GAATCCTGTC CAACGTTACG AATGCGCCCA AAATCCAGCG AGTCGGTTGC GGCCAATGGA 
CAAGAGATGA TCGGCGTCAT TGTCAGCAGC ACGGGCAACG GCAGTAGCAG CAGTAATAAT 
AGCTGTAGTA GCGCTGGTAG CACCAAGAAG CGAGAGGATC TCACCAGTCT TGGATCGGAT 
GATTCTGGCA TCATCTGTGG CTCAGAGTCG GACCAAATAT CTCTGAATCG CATATGCCAC 
TCACATGAGT CGCTTGATTC GGGTGAGATG GATGCGGATG CCGATGCAGA AGAGGAGTGC 
GTTGATCTTA TGGATACGAC ATCCATCGAC GAGGAGTACA ACATTATGCA GCCGGACCAT 
CTCTGCCTCT ATCCTCCACA GGCCGCTTCC ACCGAATTGG ACTGCCGCAC CCTGCGTCCG 
TCTCGGAAAC AAAAGCGACA GCAACAGCAG GTCCTGGAGC GGAAGAATCT ACCGTCCCAA 
AACCAGATCG AAAGCGAAGA CTCAGTGGAT GCTGGCGTCG ACGCCCGAAC ACGCTATCGC 
GTTATGAACA TGTCGCAGGC AGCTATCAAT GTTGAACTCG GAACAGCTTC CAATGAAATC 
GATCCGGATG TCAACTACAG AGAATCTCAC CAAGATCCCA TCGCTGATGC ACCTTCCCTC 
TCCACCCCAA GTGTAGCATC AACGGCGTCA GCCACGCCAT CGGTAACCCC AACACCGATT 
GCCACTCCGA CACCAACGGA GAGCACCAAG AACGACCAAG TGCTGTTTAA GAATTTCTTT 
GGCGCCACCA AGAATGCCAT ATTTCGCACA GCCCAGAGCA TTATTGAAAA CCATGAGAAG 
AAAAACGCTG CTAAGCAGAA GGAGCAGCCT GATCACTCAG GGACAGTCAG CTTAGTGGGT 
TCGCCAAATG GAGGAAGCAG TGTGCCACAA GACCTGGTCA AGTCTCCCAC AGACATTTCG 
AAAAAGAAGG AATTCTTTAG TTTGCTCACG TCTAGTTCCA GCATAAAGGC AGCGGCTGCA 
GCTGCGGCGA ACTCAGCTGC TAGCACTCCT CCTGCCACGC CTACGGAGGC ACTAGCTGCG 
AATTCAAATT CGAAGCTTCA ACCGCTTTCC GATCCATCTG CCGATTTAAA AGTAAAGGAA 
CGTACTCTCA ACTTGCGTCT ACCTGGAGAT AGTGAAGGCA GTGAAGGAGC ATTGACAAAG 
TGTTCATCAA TCAACTCGAT ACACAAACTA AAGCTTCCTG TTCCTGGTGT CGTTAAGTAT 
TTCATCAGTG AAAAAGCACC TGTGTCTGAT GTGCTCCAGA AGCCAGAGAA AGGCCAAAGC 
GGCCTTTTGC GTTTCTTTGA GTCGCCAGTG TTCAACATAC ACTTTGCTGT GCATTATCTC 
TTTTACTCAA AGGAACCGGG AGTCCTCAGC TTTATCGGCA ACAAGATCTT CAGTTTTCCC 
GACCAAGAGG TTGATCTGTA CATACCACAA TTGGTTGTTA TGTACATTCA GATGGATGAG 
CTAGCTGAGG TTCTGGATCC GTATCTTACC ATTCGGTGTC GCAAGTCGGT TGACTTCTCG 
CTGAAGTGTC TGTGGCTCTT GGAGGCATAT AACTACCAAG TGGACTCGCT AGGGAACTCA 
CACAACAGTT CTCGGAAATC AAAGCTGGCG CTCATGAAGG AAATATTCTC AAAAAGGGAG 
CACAAACAGA CTCAAAACGA CCTCAAGTCT GCAGGTACTG GAGGTCTGCA TGTTGGAGAG 
CCGAGGTCGG TAGTCGCGCT TGCCAAGAAG ACTCACCACC GCTCGCAATC TGATGCCACT 
GTGCTCCTAG CCGACTTTCG CTCGCCTCAC ACACTGAGTA TATCGCATCG TATGTACCAG 
CAGCCACCCT ATACCACACA AACACTTCCC ACTACGCCCG CCAAGCTGTG CCTCGGTGAC 
TTAACTTCCG GCCACGCATT TGATAACGGG TGCACCTGCT TCGAGACGGT TCGAGGCCAA 
GTAAATGGTC TGCTTGGGCA GAGGACGCTA TGCAGCTGTG GTGCTCCAAA GACGTCACCC 
CAGAAAGAGT TTATGAAGGC ATTGATGAAC GTTGGTAAAA ATCTCACCTC GTGGCCTTCC 
AAAGCGGAGA AGACTTCAGC GCTGCGGATG TCCCTCAACT TGATTAACAA AAACCTGCCC 
GCTAGAGTCT GGCTTCCTTT GTACTCAGAC ATTCCACATC ATGTCGTTCG TATTACAGAG 
GAGAAGACCG CAGTACTGAA CTCGAAGGAT AAAACCCCCT ATATCATATA CGTAGAAGTT 
GTTGAAATTC CTGACATCTA TACTTCTCCC CTGATTCCCA AAATGATGCC TTCACTGCGG 
CACACCAAGA GCGAAGAGCA CCTGGATGGG TCCTGCCTGA ACTCCCACCA ACATCTGAGC 
TGCAGTCGAA CTTCAAGCTG CAGTAGCAGT CAGCAAGGCA CAAGTTGCCG CCGAAAAAAA 
GGAGCAGATG TAGATGGCGG CTGCGGGGAT GCGGGTCCAC ATAATAGCTG CAGCAATGTA 
CATTCGTTAG GTGGACTTCA GTTCCAGGAA GATGACGTGT GGTCACAAGA GGAGGACGAA 
ATAACAGCGC AGTATCTTAA CATGCGGAAA GTGAGCGAGC GGGACACAAT TTCACAAATA 
TCACTAGATT CCTGCGATTC AAGAGATCAA GGTCCACCAG TCGTGTTTAA TATTGGCGAC 
GTGCGCTTGC GCCACTGCAG CAATTTGAGC TGCGAAAATA CTAAATCCTT CAGTAACGAC 
CCGGAGGATC CTTCGGCGGC AGCTTTAAAG GAACCGTGGC ACGAAAAGGA AAAGCTTATC 
CGCGAGTCTT CGCCATACGG CCATCTATCA AACTGGCGAC TGCTTTCCGC CATCGTAAAG 
TGTGGCGATG ATTTACGCCA AGAACTAATG GCTACCCAAC TTTTACAGAT GTTTAAAATT 
ATTTGGCAAG AAGAGCAAGT AGATCTGTGG GTGCGCCCGT ACAAAATAGT CTGCCTCTCG 
AATGATAGTG GTCTTATAGA GCCGATCCTT AACACTGTAT CCCTGCACCA AATTAAGAAG 
AACTCCAACA AATCGTTGAA AGAGTATTTT ATCGATGAAT ACGGCTCACC AAGCGGAGAA 
AGTTTTCGGC GCGCGCAGAA GAACTTCGTC CAAAGCTGTG CAGCTTACTG TCTAATTTCA 
TATTTACTGC AAGTTAAGGA CAGGCATAAT GGCAACATTC TCTTCCATTC GGATGGGCAC 
ATTATACACA TTGACTTTGG ATTTATTTTA AGTATATCAC CTAAAAACTT GGGATTCGAA 
CAGTCGCCCT TTAAGCTAAC GCCGGAATTT GTTGAGGTGA TGGGTGGCAC GAGCTCGGAG 
CATTGGCGTG AATTTAATAA GCTCTTGTTA GTTGGAATGA TGTCTGCTCG AAAGCATATG 
GATCGTATTA TTAACTTCGT GGAAATTATG CGCAGCAATG CGCATTTGCC GTGCTTTAAG 
AATGGGTGCT CTGGAACCGT ACAGAATCTC CGAAAACGCT TTCATATGAA TTTAACCGAA 
CAAGAAATGG AGCGAAAGGT TGAGCAGCTT GTACAGGACT CGTTAAAAAG TCTGTCAACC 
AAACTGTACG ACGGTTATCA ATACTACACT AATGGCATAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261203.1
CDS	952..5976
Translation	MNSVISAFKGKKNSNPSCNSPMGSANGTAPVEAKPNNCYQYLRHIREIDTNNKLLSPVVVVAEERPVSKAGSPSTVATMATGVDVVDHCLPTTVRLSVTEALSDLNMNGKAVEPPVTPCTSVGDNANLEVAETWACPPPQATDDEDISEHSTAACTSTSSSSEHSNSTVVHSPTVGESTCDSVSVSVSHPSPSVSQNNTSSATGRMSSPHSQSSLSIACEPHSASTTTLSSLGSDLGNGNGSTVPAFLMAAPVTLPIGGQMPTIKCLPGASSAASTPKHSRYSKPRLSLSRVFNHSMPSVHGRPNLSLAPAGVPGSVNPPKRISTHQRNLSLDFRSMGILLPPVSQVTNNHINHTQHHRNRSLDSALQRIPEVEVSSPNAESENTFCSPSILGATMCSKIATTAATVTSALSPPGTPTPALMSTACTPTIGAVKGNVQHPVPKHEQSISLAAACRSSAVVESCPTLRMRPKSSESVAANGQEMIGVIVSSTGNGSSSSNNSCSSAGSTKKREDLTSLGSDDSGIICGSESDQISLNRICHSHESLDSGEMDADADAEEECVDLMDTTSIDEEYNIMQPDHLCLYPPQAASTELDCRTLRPSRKQKRQQQQVLERKNLPSQNQIESEDSVDAGVDARTRYRVMNMSQAAINVELGTASNEIDPDVNYRESHQDPIADAPSLSTPSVASTASATPSVTPTPIATPTPTESTKNDQVLFKNFFGATKNAIFRTAQSIIENHEKKNAAKQKEQPDHSGTVSLVGSPNGGSSVPQDLVKSPTDISKKKEFFSLLTSSSSIKAAAAAAANSAASTPPATPTEALAANSNSKLQPLSDPSADLKVKERTLNLRLPGDSEGSEGALTKCSSINSIHKLKLPVPGVVKYFISEKAPVSDVLQKPEKGQSGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFSFPDQEVDLYIPQLVVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNSHNSSRKSKLALMKEIFSKREHKQTQNDLKSAGTGGLHVGEPRSVVALAKKTHHRSQSDATVLLADFRSPHTLSISHRMYQQPPYTTQTLPTTPAKLCLGDLTSGHAFDNGCTCFETVRGQVNGLLGQRTLCSCGAPKTSPQKEFMKALMNVGKNLTSWPSKAEKTSALRMSLNLINKNLPARVWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPKMMPSLRHTKSEEHLDGSCLNSHQHLSCSRTSSCSSSQQGTSCRRKKGADVDGGCGDAGPHNSCSNVHSLGGLQFQEDDVWSQEEDEITAQYLNMRKVSERDTISQISLDSCDSRDQGPPVVFNIGDVRLRHCSNLSCENTKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKKNSNKSLKEYFIDEYGSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISPKNLGFEQSPFKLTPEFVEVMGGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRSNAHLPCFKNGCSGTVQNLRKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGIL

Target ORF information:

RefSeq Version	NM_001274274.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster four wheel drive (fwd), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274274.1

ORF Insert Sequence:

ATGAACTCCG TAATATCAGC ATTTAAAGGA AAAAAAAATA GCAATCCATC CTGCAATTCT 
CCAATGGGAT CGGCGAATGG CACTGCTCCG GTGGAGGCAA AGCCAAACAA TTGCTACCAG 
TACCTGCGCC ACATACGCGA AATCGATACA AACAACAAGC TCCTTTCTCC CGTCGTGGTT 
GTTGCAGAGG AGAGGCCAGT GAGCAAGGCT GGTTCGCCCT CGACAGTGGC GACCATGGCT 
ACTGGAGTCG ATGTGGTGGA TCACTGCCTG CCCACAACGG TACGATTGTC CGTTACCGAG 
GCGCTATCCG ACTTGAACAT GAACGGAAAA GCCGTCGAAC CCCCCGTCAC CCCATGCACT 
TCGGTTGGCG ACAATGCCAA TCTGGAGGTG GCTGAAACAT GGGCTTGTCC ACCGCCACAA 
GCGACGGATG ACGAGGACAT ATCCGAGCAC TCGACAGCCG CCTGCACCAG CACAAGTAGC 
TCCAGCGAGC ACAGCAACAG CACCGTGGTG CACAGTCCAA CTGTGGGGGA GAGCACCTGT 
GACTCCGTGT CCGTCTCGGT AAGCCATCCA AGTCCCAGCG TGAGTCAGAA CAACACATCG 
TCCGCCACCG GCCGGATGAG TAGCCCCCAC AGCCAGTCGT CGCTCTCCAT AGCCTGCGAG 
CCGCACTCGG CGTCGACCAC CACGCTGTCG TCGCTGGGAT CAGACCTGGG CAATGGAAAC 
GGAAGTACCG TACCCGCGTT TCTAATGGCC GCCCCCGTCA CGCTGCCAAT TGGCGGCCAG 
ATGCCCACGA TAAAGTGCCT CCCAGGAGCA AGCTCAGCGG CCAGCACGCC CAAGCACTCG 
CGCTACTCTA AGCCTCGTCT CAGCCTGAGC CGCGTGTTTA ATCACTCTAT GCCCTCCGTG 
CACGGCCGAC CAAATCTCAG TTTAGCGCCA GCTGGAGTTC CGGGAAGTGT GAATCCGCCG 
AAACGTATTT CCACGCACCA AAGAAACCTA AGCTTGGATT TTAGATCCAT GGGCATACTT 
CTTCCGCCTG TCTCGCAGGT GACCAATAAT CACATCAATC ACACCCAGCA CCACCGCAAT 
CGCAGCCTCG ACAGTGCCTT GCAACGCATT CCGGAGGTCG AAGTGTCATC GCCAAATGCT 
GAAAGCGAAA ACACGTTCTG CTCACCGTCC ATTCTCGGTG CGACTATGTG CTCAAAAATT 
GCCACGACAG CGGCAACTGT AACGTCAGCC CTGTCCCCAC CAGGCACGCC CACACCGGCT 
CTGATGTCGA CGGCGTGCAC GCCCACGATT GGAGCCGTAA AGGGCAACGT GCAACATCCC 
GTGCCGAAGC ATGAGCAATC GATTTCTTTG GCAGCTGCTT GCAGGTCCAG TGCCGTCGTG 
GAATCCTGTC CAACGTTACG AATGCGCCCA AAATCCAGCG AGTCGGTTGC GGCCAATGGA 
CAAGAGATGA TCGGCGTCAT TGTCAGCAGC ACGGGCAACG GCAGTAGCAG CAGTAATAAT 
AGCTGTAGTA GCGCTGGTAG CACCAAGAAG CGAGAGGATC TCACCAGTCT TGGATCGGAT 
GATTCTGGCA TCATCTGTGG CTCAGAGTCG GACCAAATAT CTCTGAATCG CATATGCCAC 
TCACATGAGT CGCTTGATTC GGGTGAGATG GATGCGGATG CCGATGCAGA AGAGGAGTGC 
GTTGATCTTA TGGATACGAC ATCCATCGAC GAGGAGTACA ACATTATGCA GCCGGACCAT 
CTCTGCCTCT ATCCTCCACA GGCCGCTTCC ACCGAATTGG ACTGCCGCAC CCTGCGTCCG 
TCTCGGAAAC AAAAGCGACA GCAACAGCAG GTCCTGGAGC GGAAGAATCT ACCGTCCCAA 
AACCAGATCG AAAGCGAAGA CTCAGTGGAT GCTGGCGTCG ACGCCCGAAC ACGCTATCGC 
GTTATGAACA TGTCGCAGGC AGCTATCAAT GTTGAACTCG GAACAGCTTC CAATGAAATC 
GATCCGGATG TCAACTACAG AGAATCTCAC CAAGATCCCA TCGCTGATGC ACCTTCCCTC 
TCCACCCCAA GTGTAGCATC AACGGCGTCA GCCACGCCAT CGGTAACCCC AACACCGATT 
GCCACTCCGA CACCAACGGA GAGCACCAAG AACGACCAAG TGCTGTTTAA GAATTTCTTT 
GGCGCCACCA AGAATGCCAT ATTTCGCACA GCCCAGAGCA TTATTGAAAA CCATGAGAAG 
AAAAACGCTG CTAAGCAGAA GGAGCAGCCT GATCACTCAG GGACAGTCAG CTTAGTGGGT 
TCGCCAAATG GAGGAAGCAG TGTGCCACAA GACCTGGTCA AGTCTCCCAC AGACATTTCG 
AAAAAGAAGG AATTCTTTAG TTTGCTCACG TCTAGTTCCA GCATAAAGGC AGCGGCTGCA 
GCTGCGGCGA ACTCAGCTGC TAGCACTCCT CCTGCCACGC CTACGGAGGC ACTAGCTGCG 
AATTCAAATT CGAAGCTTCA ACCGCTTTCC GATCCATCTG CCGATTTAAA AGTAAAGGAA 
CGTACTCTCA ACTTGCGTCT ACCTGGAGAT AGTGAAGGCA GTGAAGGAGC ATTGACAAAG 
TGTTCATCAA TCAACTCGAT ACACAAACTA AAGCTTCCTG TTCCTGGTGT CGTTAAGTAT 
TTCATCAGTG AAAAAGCACC TGTGTCTGAT GTGCTCCAGA AGCCAGAGAA AGGCCAAAGC 
GGCCTTTTGC GTTTCTTTGA GTCGCCAGTG TTCAACATAC ACTTTGCTGT GCATTATCTC 
TTTTACTCAA AGGAACCGGG AGTCCTCAGC TTTATCGGCA ACAAGATCTT CAGTTTTCCC 
GACCAAGAGG TTGATCTGTA CATACCACAA TTGGTTGTTA TGTACATTCA GATGGATGAG 
CTAGCTGAGG TTCTGGATCC GTATCTTACC ATTCGGTGTC GCAAGTCGGT TGACTTCTCG 
CTGAAGTGTC TGTGGCTCTT GGAGGCATAT AACTACCAAG TGGACTCGCT AGGGAACTCA 
CACAACAGTT CTCGGAAATC AAAGCTGGCG CTCATGAAGG AAATATTCTC AAAAAGGGAG 
CACAAACAGA CTCAAAACGA CCTCAAGTCT GCAGGTACTG GAGGTCTGCA TGTTGGAGAG 
CCGAGGTCGG TAGTCGCGCT TGCCAAGAAG ACTCACCACC GCTCGCAATC TGATGCCACT 
GTGCTCCTAG CCGACTTTCG CTCGCCTCAC ACACTGAGTA TATCGCATCG TATGTACCAG 
CAGCCACCCT ATACCACACA AACACTTCCC ACTACGCCCG CCAAGCTGTG CCTCGGTGAC 
TTAACTTCCG GCCACGCATT TGATAACGGG TGCACCTGCT TCGAGACGGT TCGAGGCCAA 
GTAAATGGTC TGCTTGGGCA GAGGACGCTA TGCAGCTGTG GTGCTCCAAA GACGTCACCC 
CAGAAAGAGT TTATGAAGGC ATTGATGAAC GTTGGTAAAA ATCTCACCTC GTGGCCTTCC 
AAAGCGGAGA AGACTTCAGC GCTGCGGATG TCCCTCAACT TGATTAACAA AAACCTGCCC 
GCTAGAGTCT GGCTTCCTTT GTACTCAGAC ATTCCACATC ATGTCGTTCG TATTACAGAG 
GAGAAGACCG CAGTACTGAA CTCGAAGGAT AAAACCCCCT ATATCATATA CGTAGAAGTT 
GTTGAAATTC CTGACATCTA TACTTCTCCC CTGATTCCCA AAATGATGCC TTCACTGCGG 
CACACCAAGA GCGAAGAGCA CCTGGATGGG TCCTGCCTGA ACTCCCACCA ACATCTGAGC 
TGCAGTCGAA CTTCAAGCTG CAGTAGCAGT CAGCAAGGCA CAAGTTGCCG CCGAAAAAAA 
GGAGCAGATG TAGATGGCGG CTGCGGGGAT GCGGGTCCAC ATAATAGCTG CAGCAATGTA 
CATTCGTTAG GTGGACTTCA GTTCCAGGAA GATGACGTGT GGTCACAAGA GGAGGACGAA 
ATAACAGCGC AGTATCTTAA CATGCGGAAA GTGAGCGAGC GGGACACAAT TTCACAAATA 
TCACTAGATT CCTGCGATTC AAGAGATCAA GGTCCACCAG TCGTGTTTAA TATTGGCGAC 
GTGCGCTTGC GCCACTGCAG CAATTTGAGC TGCGAAAATA CTAAATCCTT CAGTAACGAC 
CCGGAGGATC CTTCGGCGGC AGCTTTAAAG GAACCGTGGC ACGAAAAGGA AAAGCTTATC 
CGCGAGTCTT CGCCATACGG CCATCTATCA AACTGGCGAC TGCTTTCCGC CATCGTAAAG 
TGTGGCGATG ATTTACGCCA AGAACTAATG GCTACCCAAC TTTTACAGAT GTTTAAAATT 
ATTTGGCAAG AAGAGCAAGT AGATCTGTGG GTGCGCCCGT ACAAAATAGT CTGCCTCTCG 
AATGATAGTG GTCTTATAGA GCCGATCCTT AACACTGTAT CCCTGCACCA AATTAAGAAG 
AACTCCAACA AATCGTTGAA AGAGTATTTT ATCGATGAAT ACGGCTCACC AAGCGGAGAA 
AGTTTTCGGC GCGCGCAGAA GAACTTCGTC CAAAGCTGTG CAGCTTACTG TCTAATTTCA 
TATTTACTGC AAGTTAAGGA CAGGCATAAT GGCAACATTC TCTTCCATTC GGATGGGCAC 
ATTATACACA TTGACTTTGG ATTTATTTTA AGTATATCAC CTAAAAACTT GGGATTCGAA 
CAGTCGCCCT TTAAGCTAAC GCCGGAATTT GTTGAGGTGA TGGGTGGCAC GAGCTCGGAG 
CATTGGCGTG AATTTAATAA GCTCTTGTTA GTTGGAATGA TGTCTGCTCG AAAGCATATG 
GATCGTATTA TTAACTTCGT GGAAATTATG CGCAGCAATG CGCATTTGCC GTGCTTTAAG 
AATGGGTGCT CTGGAACCGT ACAGAATCTC CGAAAACGCT TTCATATGAA TTTAACCGAA 
CAAGAAATGG AGCGAAAGGT TGAGCAGCTT GTACAGGACT CGTTAAAAAG TCTGTCAACC 
AAACTGTACG ACGGTTATCA ATACTACACT AATGGCATAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fwd ( NM_080083.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524822.1 Drosophila melanogaster four wheel drive (fwd), transcript variant A, mRNA.

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CloneID	OFa19402
Clone ID Related Accession (Same CDS sequence)	NM_167826.2 , NM_001274274.1 , NM_080083.4
Accession Version	NM_080083.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	four wheel drive, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080083.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCCG TAATATCAGC ATTTAAAGGA AAAAAAAATA GCAATCCATC CTGCAATTCT 
CCAATGGGAT CGGCGAATGG CACTGCTCCG GTGGAGGCAA AGCCAAACAA TTGCTACCAG 
TACCTGCGCC ACATACGCGA AATCGATACA AACAACAAGC TCCTTTCTCC CGTCGTGGTT 
GTTGCAGAGG AGAGGCCAGT GAGCAAGGCT GGTTCGCCCT CGACAGTGGC GACCATGGCT 
ACTGGAGTCG ATGTGGTGGA TCACTGCCTG CCCACAACGG TACGATTGTC CGTTACCGAG 
GCGCTATCCG ACTTGAACAT GAACGGAAAA GCCGTCGAAC CCCCCGTCAC CCCATGCACT 
TCGGTTGGCG ACAATGCCAA TCTGGAGGTG GCTGAAACAT GGGCTTGTCC ACCGCCACAA 
GCGACGGATG ACGAGGACAT ATCCGAGCAC TCGACAGCCG CCTGCACCAG CACAAGTAGC 
TCCAGCGAGC ACAGCAACAG CACCGTGGTG CACAGTCCAA CTGTGGGGGA GAGCACCTGT 
GACTCCGTGT CCGTCTCGGT AAGCCATCCA AGTCCCAGCG TGAGTCAGAA CAACACATCG 
TCCGCCACCG GCCGGATGAG TAGCCCCCAC AGCCAGTCGT CGCTCTCCAT AGCCTGCGAG 
CCGCACTCGG CGTCGACCAC CACGCTGTCG TCGCTGGGAT CAGACCTGGG CAATGGAAAC 
GGAAGTACCG TACCCGCGTT TCTAATGGCC GCCCCCGTCA CGCTGCCAAT TGGCGGCCAG 
ATGCCCACGA TAAAGTGCCT CCCAGGAGCA AGCTCAGCGG CCAGCACGCC CAAGCACTCG 
CGCTACTCTA AGCCTCGTCT CAGCCTGAGC CGCGTGTTTA ATCACTCTAT GCCCTCCGTG 
CACGGCCGAC CAAATCTCAG TTTAGCGCCA GCTGGAGTTC CGGGAAGTGT GAATCCGCCG 
AAACGTATTT CCACGCACCA AAGAAACCTA AGCTTGGATT TTAGATCCAT GGGCATACTT 
CTTCCGCCTG TCTCGCAGGT GACCAATAAT CACATCAATC ACACCCAGCA CCACCGCAAT 
CGCAGCCTCG ACAGTGCCTT GCAACGCATT CCGGAGGTCG AAGTGTCATC GCCAAATGCT 
GAAAGCGAAA ACACGTTCTG CTCACCGTCC ATTCTCGGTG CGACTATGTG CTCAAAAATT 
GCCACGACAG CGGCAACTGT AACGTCAGCC CTGTCCCCAC CAGGCACGCC CACACCGGCT 
CTGATGTCGA CGGCGTGCAC GCCCACGATT GGAGCCGTAA AGGGCAACGT GCAACATCCC 
GTGCCGAAGC ATGAGCAATC GATTTCTTTG GCAGCTGCTT GCAGGTCCAG TGCCGTCGTG 
GAATCCTGTC CAACGTTACG AATGCGCCCA AAATCCAGCG AGTCGGTTGC GGCCAATGGA 
CAAGAGATGA TCGGCGTCAT TGTCAGCAGC ACGGGCAACG GCAGTAGCAG CAGTAATAAT 
AGCTGTAGTA GCGCTGGTAG CACCAAGAAG CGAGAGGATC TCACCAGTCT TGGATCGGAT 
GATTCTGGCA TCATCTGTGG CTCAGAGTCG GACCAAATAT CTCTGAATCG CATATGCCAC 
TCACATGAGT CGCTTGATTC GGGTGAGATG GATGCGGATG CCGATGCAGA AGAGGAGTGC 
GTTGATCTTA TGGATACGAC ATCCATCGAC GAGGAGTACA ACATTATGCA GCCGGACCAT 
CTCTGCCTCT ATCCTCCACA GGCCGCTTCC ACCGAATTGG ACTGCCGCAC CCTGCGTCCG 
TCTCGGAAAC AAAAGCGACA GCAACAGCAG GTCCTGGAGC GGAAGAATCT ACCGTCCCAA 
AACCAGATCG AAAGCGAAGA CTCAGTGGAT GCTGGCGTCG ACGCCCGAAC ACGCTATCGC 
GTTATGAACA TGTCGCAGGC AGCTATCAAT GTTGAACTCG GAACAGCTTC CAATGAAATC 
GATCCGGATG TCAACTACAG AGAATCTCAC CAAGATCCCA TCGCTGATGC ACCTTCCCTC 
TCCACCCCAA GTGTAGCATC AACGGCGTCA GCCACGCCAT CGGTAACCCC AACACCGATT 
GCCACTCCGA CACCAACGGA GAGCACCAAG AACGACCAAG TGCTGTTTAA GAATTTCTTT 
GGCGCCACCA AGAATGCCAT ATTTCGCACA GCCCAGAGCA TTATTGAAAA CCATGAGAAG 
AAAAACGCTG CTAAGCAGAA GGAGCAGCCT GATCACTCAG GGACAGTCAG CTTAGTGGGT 
TCGCCAAATG GAGGAAGCAG TGTGCCACAA GACCTGGTCA AGTCTCCCAC AGACATTTCG 
AAAAAGAAGG AATTCTTTAG TTTGCTCACG TCTAGTTCCA GCATAAAGGC AGCGGCTGCA 
GCTGCGGCGA ACTCAGCTGC TAGCACTCCT CCTGCCACGC CTACGGAGGC ACTAGCTGCG 
AATTCAAATT CGAAGCTTCA ACCGCTTTCC GATCCATCTG CCGATTTAAA AGTAAAGGAA 
CGTACTCTCA ACTTGCGTCT ACCTGGAGAT AGTGAAGGCA GTGAAGGAGC ATTGACAAAG 
TGTTCATCAA TCAACTCGAT ACACAAACTA AAGCTTCCTG TTCCTGGTGT CGTTAAGTAT 
TTCATCAGTG AAAAAGCACC TGTGTCTGAT GTGCTCCAGA AGCCAGAGAA AGGCCAAAGC 
GGCCTTTTGC GTTTCTTTGA GTCGCCAGTG TTCAACATAC ACTTTGCTGT GCATTATCTC 
TTTTACTCAA AGGAACCGGG AGTCCTCAGC TTTATCGGCA ACAAGATCTT CAGTTTTCCC 
GACCAAGAGG TTGATCTGTA CATACCACAA TTGGTTGTTA TGTACATTCA GATGGATGAG 
CTAGCTGAGG TTCTGGATCC GTATCTTACC ATTCGGTGTC GCAAGTCGGT TGACTTCTCG 
CTGAAGTGTC TGTGGCTCTT GGAGGCATAT AACTACCAAG TGGACTCGCT AGGGAACTCA 
CACAACAGTT CTCGGAAATC AAAGCTGGCG CTCATGAAGG AAATATTCTC AAAAAGGGAG 
CACAAACAGA CTCAAAACGA CCTCAAGTCT GCAGGTACTG GAGGTCTGCA TGTTGGAGAG 
CCGAGGTCGG TAGTCGCGCT TGCCAAGAAG ACTCACCACC GCTCGCAATC TGATGCCACT 
GTGCTCCTAG CCGACTTTCG CTCGCCTCAC ACACTGAGTA TATCGCATCG TATGTACCAG 
CAGCCACCCT ATACCACACA AACACTTCCC ACTACGCCCG CCAAGCTGTG CCTCGGTGAC 
TTAACTTCCG GCCACGCATT TGATAACGGG TGCACCTGCT TCGAGACGGT TCGAGGCCAA 
GTAAATGGTC TGCTTGGGCA GAGGACGCTA TGCAGCTGTG GTGCTCCAAA GACGTCACCC 
CAGAAAGAGT TTATGAAGGC ATTGATGAAC GTTGGTAAAA ATCTCACCTC GTGGCCTTCC 
AAAGCGGAGA AGACTTCAGC GCTGCGGATG TCCCTCAACT TGATTAACAA AAACCTGCCC 
GCTAGAGTCT GGCTTCCTTT GTACTCAGAC ATTCCACATC ATGTCGTTCG TATTACAGAG 
GAGAAGACCG CAGTACTGAA CTCGAAGGAT AAAACCCCCT ATATCATATA CGTAGAAGTT 
GTTGAAATTC CTGACATCTA TACTTCTCCC CTGATTCCCA AAATGATGCC TTCACTGCGG 
CACACCAAGA GCGAAGAGCA CCTGGATGGG TCCTGCCTGA ACTCCCACCA ACATCTGAGC 
TGCAGTCGAA CTTCAAGCTG CAGTAGCAGT CAGCAAGGCA CAAGTTGCCG CCGAAAAAAA 
GGAGCAGATG TAGATGGCGG CTGCGGGGAT GCGGGTCCAC ATAATAGCTG CAGCAATGTA 
CATTCGTTAG GTGGACTTCA GTTCCAGGAA GATGACGTGT GGTCACAAGA GGAGGACGAA 
ATAACAGCGC AGTATCTTAA CATGCGGAAA GTGAGCGAGC GGGACACAAT TTCACAAATA 
TCACTAGATT CCTGCGATTC AAGAGATCAA GGTCCACCAG TCGTGTTTAA TATTGGCGAC 
GTGCGCTTGC GCCACTGCAG CAATTTGAGC TGCGAAAATA CTAAATCCTT CAGTAACGAC 
CCGGAGGATC CTTCGGCGGC AGCTTTAAAG GAACCGTGGC ACGAAAAGGA AAAGCTTATC 
CGCGAGTCTT CGCCATACGG CCATCTATCA AACTGGCGAC TGCTTTCCGC CATCGTAAAG 
TGTGGCGATG ATTTACGCCA AGAACTAATG GCTACCCAAC TTTTACAGAT GTTTAAAATT 
ATTTGGCAAG AAGAGCAAGT AGATCTGTGG GTGCGCCCGT ACAAAATAGT CTGCCTCTCG 
AATGATAGTG GTCTTATAGA GCCGATCCTT AACACTGTAT CCCTGCACCA AATTAAGAAG 
AACTCCAACA AATCGTTGAA AGAGTATTTT ATCGATGAAT ACGGCTCACC AAGCGGAGAA 
AGTTTTCGGC GCGCGCAGAA GAACTTCGTC CAAAGCTGTG CAGCTTACTG TCTAATTTCA 
TATTTACTGC AAGTTAAGGA CAGGCATAAT GGCAACATTC TCTTCCATTC GGATGGGCAC 
ATTATACACA TTGACTTTGG ATTTATTTTA AGTATATCAC CTAAAAACTT GGGATTCGAA 
CAGTCGCCCT TTAAGCTAAC GCCGGAATTT GTTGAGGTGA TGGGTGGCAC GAGCTCGGAG 
CATTGGCGTG AATTTAATAA GCTCTTGTTA GTTGGAATGA TGTCTGCTCG AAAGCATATG 
GATCGTATTA TTAACTTCGT GGAAATTATG CGCAGCAATG CGCATTTGCC GTGCTTTAAG 
AATGGGTGCT CTGGAACCGT ACAGAATCTC CGAAAACGCT TTCATATGAA TTTAACCGAA 
CAAGAAATGG AGCGAAAGGT TGAGCAGCTT GTACAGGACT CGTTAAAAAG TCTGTCAACC 
AAACTGTACG ACGGTTATCA ATACTACACT AATGGCATAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524822.1
CDS	1185..6209
Translation	MNSVISAFKGKKNSNPSCNSPMGSANGTAPVEAKPNNCYQYLRHIREIDTNNKLLSPVVVVAEERPVSKAGSPSTVATMATGVDVVDHCLPTTVRLSVTEALSDLNMNGKAVEPPVTPCTSVGDNANLEVAETWACPPPQATDDEDISEHSTAACTSTSSSSEHSNSTVVHSPTVGESTCDSVSVSVSHPSPSVSQNNTSSATGRMSSPHSQSSLSIACEPHSASTTTLSSLGSDLGNGNGSTVPAFLMAAPVTLPIGGQMPTIKCLPGASSAASTPKHSRYSKPRLSLSRVFNHSMPSVHGRPNLSLAPAGVPGSVNPPKRISTHQRNLSLDFRSMGILLPPVSQVTNNHINHTQHHRNRSLDSALQRIPEVEVSSPNAESENTFCSPSILGATMCSKIATTAATVTSALSPPGTPTPALMSTACTPTIGAVKGNVQHPVPKHEQSISLAAACRSSAVVESCPTLRMRPKSSESVAANGQEMIGVIVSSTGNGSSSSNNSCSSAGSTKKREDLTSLGSDDSGIICGSESDQISLNRICHSHESLDSGEMDADADAEEECVDLMDTTSIDEEYNIMQPDHLCLYPPQAASTELDCRTLRPSRKQKRQQQQVLERKNLPSQNQIESEDSVDAGVDARTRYRVMNMSQAAINVELGTASNEIDPDVNYRESHQDPIADAPSLSTPSVASTASATPSVTPTPIATPTPTESTKNDQVLFKNFFGATKNAIFRTAQSIIENHEKKNAAKQKEQPDHSGTVSLVGSPNGGSSVPQDLVKSPTDISKKKEFFSLLTSSSSIKAAAAAAANSAASTPPATPTEALAANSNSKLQPLSDPSADLKVKERTLNLRLPGDSEGSEGALTKCSSINSIHKLKLPVPGVVKYFISEKAPVSDVLQKPEKGQSGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFSFPDQEVDLYIPQLVVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNSHNSSRKSKLALMKEIFSKREHKQTQNDLKSAGTGGLHVGEPRSVVALAKKTHHRSQSDATVLLADFRSPHTLSISHRMYQQPPYTTQTLPTTPAKLCLGDLTSGHAFDNGCTCFETVRGQVNGLLGQRTLCSCGAPKTSPQKEFMKALMNVGKNLTSWPSKAEKTSALRMSLNLINKNLPARVWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPKMMPSLRHTKSEEHLDGSCLNSHQHLSCSRTSSCSSSQQGTSCRRKKGADVDGGCGDAGPHNSCSNVHSLGGLQFQEDDVWSQEEDEITAQYLNMRKVSERDTISQISLDSCDSRDQGPPVVFNIGDVRLRHCSNLSCENTKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKKNSNKSLKEYFIDEYGSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISPKNLGFEQSPFKLTPEFVEVMGGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRSNAHLPCFKNGCSGTVQNLRKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGIL

Target ORF information:

RefSeq Version	NM_080083.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster four wheel drive (fwd), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080083.4

ORF Insert Sequence:

ATGAACTCCG TAATATCAGC ATTTAAAGGA AAAAAAAATA GCAATCCATC CTGCAATTCT 
CCAATGGGAT CGGCGAATGG CACTGCTCCG GTGGAGGCAA AGCCAAACAA TTGCTACCAG 
TACCTGCGCC ACATACGCGA AATCGATACA AACAACAAGC TCCTTTCTCC CGTCGTGGTT 
GTTGCAGAGG AGAGGCCAGT GAGCAAGGCT GGTTCGCCCT CGACAGTGGC GACCATGGCT 
ACTGGAGTCG ATGTGGTGGA TCACTGCCTG CCCACAACGG TACGATTGTC CGTTACCGAG 
GCGCTATCCG ACTTGAACAT GAACGGAAAA GCCGTCGAAC CCCCCGTCAC CCCATGCACT 
TCGGTTGGCG ACAATGCCAA TCTGGAGGTG GCTGAAACAT GGGCTTGTCC ACCGCCACAA 
GCGACGGATG ACGAGGACAT ATCCGAGCAC TCGACAGCCG CCTGCACCAG CACAAGTAGC 
TCCAGCGAGC ACAGCAACAG CACCGTGGTG CACAGTCCAA CTGTGGGGGA GAGCACCTGT 
GACTCCGTGT CCGTCTCGGT AAGCCATCCA AGTCCCAGCG TGAGTCAGAA CAACACATCG 
TCCGCCACCG GCCGGATGAG TAGCCCCCAC AGCCAGTCGT CGCTCTCCAT AGCCTGCGAG 
CCGCACTCGG CGTCGACCAC CACGCTGTCG TCGCTGGGAT CAGACCTGGG CAATGGAAAC 
GGAAGTACCG TACCCGCGTT TCTAATGGCC GCCCCCGTCA CGCTGCCAAT TGGCGGCCAG 
ATGCCCACGA TAAAGTGCCT CCCAGGAGCA AGCTCAGCGG CCAGCACGCC CAAGCACTCG 
CGCTACTCTA AGCCTCGTCT CAGCCTGAGC CGCGTGTTTA ATCACTCTAT GCCCTCCGTG 
CACGGCCGAC CAAATCTCAG TTTAGCGCCA GCTGGAGTTC CGGGAAGTGT GAATCCGCCG 
AAACGTATTT CCACGCACCA AAGAAACCTA AGCTTGGATT TTAGATCCAT GGGCATACTT 
CTTCCGCCTG TCTCGCAGGT GACCAATAAT CACATCAATC ACACCCAGCA CCACCGCAAT 
CGCAGCCTCG ACAGTGCCTT GCAACGCATT CCGGAGGTCG AAGTGTCATC GCCAAATGCT 
GAAAGCGAAA ACACGTTCTG CTCACCGTCC ATTCTCGGTG CGACTATGTG CTCAAAAATT 
GCCACGACAG CGGCAACTGT AACGTCAGCC CTGTCCCCAC CAGGCACGCC CACACCGGCT 
CTGATGTCGA CGGCGTGCAC GCCCACGATT GGAGCCGTAA AGGGCAACGT GCAACATCCC 
GTGCCGAAGC ATGAGCAATC GATTTCTTTG GCAGCTGCTT GCAGGTCCAG TGCCGTCGTG 
GAATCCTGTC CAACGTTACG AATGCGCCCA AAATCCAGCG AGTCGGTTGC GGCCAATGGA 
CAAGAGATGA TCGGCGTCAT TGTCAGCAGC ACGGGCAACG GCAGTAGCAG CAGTAATAAT 
AGCTGTAGTA GCGCTGGTAG CACCAAGAAG CGAGAGGATC TCACCAGTCT TGGATCGGAT 
GATTCTGGCA TCATCTGTGG CTCAGAGTCG GACCAAATAT CTCTGAATCG CATATGCCAC 
TCACATGAGT CGCTTGATTC GGGTGAGATG GATGCGGATG CCGATGCAGA AGAGGAGTGC 
GTTGATCTTA TGGATACGAC ATCCATCGAC GAGGAGTACA ACATTATGCA GCCGGACCAT 
CTCTGCCTCT ATCCTCCACA GGCCGCTTCC ACCGAATTGG ACTGCCGCAC CCTGCGTCCG 
TCTCGGAAAC AAAAGCGACA GCAACAGCAG GTCCTGGAGC GGAAGAATCT ACCGTCCCAA 
AACCAGATCG AAAGCGAAGA CTCAGTGGAT GCTGGCGTCG ACGCCCGAAC ACGCTATCGC 
GTTATGAACA TGTCGCAGGC AGCTATCAAT GTTGAACTCG GAACAGCTTC CAATGAAATC 
GATCCGGATG TCAACTACAG AGAATCTCAC CAAGATCCCA TCGCTGATGC ACCTTCCCTC 
TCCACCCCAA GTGTAGCATC AACGGCGTCA GCCACGCCAT CGGTAACCCC AACACCGATT 
GCCACTCCGA CACCAACGGA GAGCACCAAG AACGACCAAG TGCTGTTTAA GAATTTCTTT 
GGCGCCACCA AGAATGCCAT ATTTCGCACA GCCCAGAGCA TTATTGAAAA CCATGAGAAG 
AAAAACGCTG CTAAGCAGAA GGAGCAGCCT GATCACTCAG GGACAGTCAG CTTAGTGGGT 
TCGCCAAATG GAGGAAGCAG TGTGCCACAA GACCTGGTCA AGTCTCCCAC AGACATTTCG 
AAAAAGAAGG AATTCTTTAG TTTGCTCACG TCTAGTTCCA GCATAAAGGC AGCGGCTGCA 
GCTGCGGCGA ACTCAGCTGC TAGCACTCCT CCTGCCACGC CTACGGAGGC ACTAGCTGCG 
AATTCAAATT CGAAGCTTCA ACCGCTTTCC GATCCATCTG CCGATTTAAA AGTAAAGGAA 
CGTACTCTCA ACTTGCGTCT ACCTGGAGAT AGTGAAGGCA GTGAAGGAGC ATTGACAAAG 
TGTTCATCAA TCAACTCGAT ACACAAACTA AAGCTTCCTG TTCCTGGTGT CGTTAAGTAT 
TTCATCAGTG AAAAAGCACC TGTGTCTGAT GTGCTCCAGA AGCCAGAGAA AGGCCAAAGC 
GGCCTTTTGC GTTTCTTTGA GTCGCCAGTG TTCAACATAC ACTTTGCTGT GCATTATCTC 
TTTTACTCAA AGGAACCGGG AGTCCTCAGC TTTATCGGCA ACAAGATCTT CAGTTTTCCC 
GACCAAGAGG TTGATCTGTA CATACCACAA TTGGTTGTTA TGTACATTCA GATGGATGAG 
CTAGCTGAGG TTCTGGATCC GTATCTTACC ATTCGGTGTC GCAAGTCGGT TGACTTCTCG 
CTGAAGTGTC TGTGGCTCTT GGAGGCATAT AACTACCAAG TGGACTCGCT AGGGAACTCA 
CACAACAGTT CTCGGAAATC AAAGCTGGCG CTCATGAAGG AAATATTCTC AAAAAGGGAG 
CACAAACAGA CTCAAAACGA CCTCAAGTCT GCAGGTACTG GAGGTCTGCA TGTTGGAGAG 
CCGAGGTCGG TAGTCGCGCT TGCCAAGAAG ACTCACCACC GCTCGCAATC TGATGCCACT 
GTGCTCCTAG CCGACTTTCG CTCGCCTCAC ACACTGAGTA TATCGCATCG TATGTACCAG 
CAGCCACCCT ATACCACACA AACACTTCCC ACTACGCCCG CCAAGCTGTG CCTCGGTGAC 
TTAACTTCCG GCCACGCATT TGATAACGGG TGCACCTGCT TCGAGACGGT TCGAGGCCAA 
GTAAATGGTC TGCTTGGGCA GAGGACGCTA TGCAGCTGTG GTGCTCCAAA GACGTCACCC 
CAGAAAGAGT TTATGAAGGC ATTGATGAAC GTTGGTAAAA ATCTCACCTC GTGGCCTTCC 
AAAGCGGAGA AGACTTCAGC GCTGCGGATG TCCCTCAACT TGATTAACAA AAACCTGCCC 
GCTAGAGTCT GGCTTCCTTT GTACTCAGAC ATTCCACATC ATGTCGTTCG TATTACAGAG 
GAGAAGACCG CAGTACTGAA CTCGAAGGAT AAAACCCCCT ATATCATATA CGTAGAAGTT 
GTTGAAATTC CTGACATCTA TACTTCTCCC CTGATTCCCA AAATGATGCC TTCACTGCGG 
CACACCAAGA GCGAAGAGCA CCTGGATGGG TCCTGCCTGA ACTCCCACCA ACATCTGAGC 
TGCAGTCGAA CTTCAAGCTG CAGTAGCAGT CAGCAAGGCA CAAGTTGCCG CCGAAAAAAA 
GGAGCAGATG TAGATGGCGG CTGCGGGGAT GCGGGTCCAC ATAATAGCTG CAGCAATGTA 
CATTCGTTAG GTGGACTTCA GTTCCAGGAA GATGACGTGT GGTCACAAGA GGAGGACGAA 
ATAACAGCGC AGTATCTTAA CATGCGGAAA GTGAGCGAGC GGGACACAAT TTCACAAATA 
TCACTAGATT CCTGCGATTC AAGAGATCAA GGTCCACCAG TCGTGTTTAA TATTGGCGAC 
GTGCGCTTGC GCCACTGCAG CAATTTGAGC TGCGAAAATA CTAAATCCTT CAGTAACGAC 
CCGGAGGATC CTTCGGCGGC AGCTTTAAAG GAACCGTGGC ACGAAAAGGA AAAGCTTATC 
CGCGAGTCTT CGCCATACGG CCATCTATCA AACTGGCGAC TGCTTTCCGC CATCGTAAAG 
TGTGGCGATG ATTTACGCCA AGAACTAATG GCTACCCAAC TTTTACAGAT GTTTAAAATT 
ATTTGGCAAG AAGAGCAAGT AGATCTGTGG GTGCGCCCGT ACAAAATAGT CTGCCTCTCG 
AATGATAGTG GTCTTATAGA GCCGATCCTT AACACTGTAT CCCTGCACCA AATTAAGAAG 
AACTCCAACA AATCGTTGAA AGAGTATTTT ATCGATGAAT ACGGCTCACC AAGCGGAGAA 
AGTTTTCGGC GCGCGCAGAA GAACTTCGTC CAAAGCTGTG CAGCTTACTG TCTAATTTCA 
TATTTACTGC AAGTTAAGGA CAGGCATAAT GGCAACATTC TCTTCCATTC GGATGGGCAC 
ATTATACACA TTGACTTTGG ATTTATTTTA AGTATATCAC CTAAAAACTT GGGATTCGAA 
CAGTCGCCCT TTAAGCTAAC GCCGGAATTT GTTGAGGTGA TGGGTGGCAC GAGCTCGGAG 
CATTGGCGTG AATTTAATAA GCTCTTGTTA GTTGGAATGA TGTCTGCTCG AAAGCATATG 
GATCGTATTA TTAACTTCGT GGAAATTATG CGCAGCAATG CGCATTTGCC GTGCTTTAAG 
AATGGGTGCT CTGGAACCGT ACAGAATCTC CGAAAACGCT TTCATATGAA TTTAACCGAA 
CAAGAAATGG AGCGAAAGGT TGAGCAGCTT GTACAGGACT CGTTAAAAAG TCTGTCAACC 
AAACTGTACG ACGGTTATCA ATACTACACT AATGGCATAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fwd ( NM_167827.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728519.1 Drosophila melanogaster four wheel drive (fwd), transcript variant C, mRNA.

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CloneID	OFa19403
Clone ID Related Accession (Same CDS sequence)	NM_167827.2
Accession Version	NM_167827.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	four wheel drive, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167827.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCATAC TTCTTCCGCC TGTCTCGCAG GTGACCAATA ATCACATCAA TCACACCCAG 
CACCACCGCA ATCGCAGCCT CGACAGTGCC TTGCAACGCA TTCCGGAGGT CGAAGTGTCA 
TCGCCAAATG CTGAAAGCGA AAACACGTTC TGCTCACCGT CCATTCTCGG TGCGACTATG 
TGCTCAAAAA TTGCCACGAC AGCGGCAACT GTAACGTCAG CCCTGTCCCC ACCAGGCACG 
CCCACACCGG CTCTGATGTC GACGGCGTGC ACGCCCACGA TTGGAGCCGT AAAGGGCAAC 
GTGCAACATC CCGTGCCGAA GCATGAGCAA TCGATTTCTT TGGCAGCTGC TTGCAGGTCC 
AGTGCCGTCG TGGAATCCTG TCCAACGTTA CGAATGCGCC CAAAATCCAG CGAGTCGGTT 
GCGGCCAATG GACAAGAGAT GATCGGCGTC ATTGTCAGCA GCACGGGCAA CGGCAGTAGC 
AGCAGTAATA ATAGCTGTAG TAGCGCTGGT AGCACCAAGA AGCGAGAGGA TCTCACCAGT 
CTTGGATCGG ATGATTCTGG CATCATCTGT GGCTCAGAGT CGGACCAAAT ATCTCTGAAT 
CGCATATGCC ACTCACATGA GTCGCTTGAT TCGGGTGAGA TGGATGCGGA TGCCGATGCA 
GAAGAGGAGT GCGTTGATCT TATGGATACG ACATCCATCG ACGAGGAGTA CAACATTATG 
CAGCCGGACC ATCTCTGCCT CTATCCTCCA CAGGCCGCTT CCACCGAATT GGACTGCCGC 
ACCCTGCGTC CGTCTCGGAA ACAAAAGCGA CAGCAACAGC AGGTCCTGGA GCGGAAGAAT 
CTACCGTCCC AAAACCAGAT CGAAAGCGAA GACTCAGTGG ATGCTGGCGT CGACGCCCGA 
ACACGCTATC GCGTTATGAA CATGTCGCAG GCAGCTATCA ATGTTGAACT CGGAACAGCT 
TCCAATGAAA TCGATCCGGA TGTCAACTAC AGAGAATCTC ACCAAGATCC CATCGCTGAT 
GCACCTTCCC TCTCCACCCC AAGTGTAGCA TCAACGGCGT CAGCCACGCC ATCGGTAACC 
CCAACACCGA TTGCCACTCC GACACCAACG GAGAGCACCA AGAACGACCA AGTGCTGTTT 
AAGAATTTCT TTGGCGCCAC CAAGAATGCC ATATTTCGCA CAGCCCAGAG CATTATTGAA 
AACCATGAGA AGAAAAACGC TGCTAAGCAG AAGGAGCAGC CTGATCACTC AGGGACAGTC 
AGCTTAGTGG GTTCGCCAAA TGGAGGAAGC AGTGTGCCAC AAGACCTGGT CAAGTCTCCC 
ACAGACATTT CGAAAAAGAA GGAATTCTTT AGTTTGCTCA CGTCTAGTTC CAGCATAAAG 
GCAGCGGCTG CAGCTGCGGC GAACTCAGCT GCTAGCACTC CTCCTGCCAC GCCTACGGAG 
GCACTAGCTG CGAATTCAAA TTCGAAGCTT CAACCGCTTT CCGATCCATC TGCCGATTTA 
AAAGTAAAGG AACGTACTCT CAACTTGCGT CTACCTGGAG ATAGTGAAGG CAGTGAAGGA 
GCATTGACAA AGTGTTCATC AATCAACTCG ATACACAAAC TAAAGCTTCC TGTTCCTGGT 
GTCGTTAAGT ATTTCATCAG TGAAAAAGCA CCTGTGTCTG ATGTGCTCCA GAAGCCAGAG 
AAAGGCCAAA GCGGCCTTTT GCGTTTCTTT GAGTCGCCAG TGTTCAACAT ACACTTTGCT 
GTGCATTATC TCTTTTACTC AAAGGAACCG GGAGTCCTCA GCTTTATCGG CAACAAGATC 
TTCAGTTTTC CCGACCAAGA GGTTGATCTG TACATACCAC AATTGGTTGT TATGTACATT 
CAGATGGATG AGCTAGCTGA GGTTCTGGAT CCGTATCTTA CCATTCGGTG TCGCAAGTCG 
GTTGACTTCT CGCTGAAGTG TCTGTGGCTC TTGGAGGCAT ATAACTACCA AGTGGACTCG 
CTAGGGAACT CACACAACAG TTCTCGGAAA TCAAAGCTGG CGCTCATGAA GGAAATATTC 
TCAAAAAGGG AGCACAAACA GACTCAAAAC GACCTCAAGT CTGCAGGTAC TGGAGGTCTG 
CATGTTGGAG AGCCGAGGTC GGTAGTCGCG CTTGCCAAGA AGACTCACCA CCGCTCGCAA 
TCTGATGCCA CTGTGCTCCT AGCCGACTTT CGCTCGCCTC ACACACTGAG TATATCGCAT 
CGTATGTACC AGCAGCCACC CTATACCACA CAAACACTTC CCACTACGCC CGCCAAGCTG 
TGCCTCGGTG ACTTAACTTC CGGCCACGCA TTTGATAACG GGTGCACCTG CTTCGAGACG 
GTTCGAGGCC AAGTAAATGG TCTGCTTGGG CAGAGGACGC TATGCAGCTG TGGTGCTCCA 
AAGACGTCAC CCCAGAAAGA GTTTATGAAG GCATTGATGA ACGTTGGTAA AAATCTCACC 
TCGTGGCCTT CCAAAGCGGA GAAGACTTCA GCGCTGCGGA TGTCCCTCAA CTTGATTAAC 
AAAAACCTGC CCGCTAGAGT CTGGCTTCCT TTGTACTCAG ACATTCCACA TCATGTCGTT 
CGTATTACAG AGGAGAAGAC CGCAGTACTG AACTCGAAGG ATAAAACCCC CTATATCATA 
TACGTAGAAG TTGTTGAAAT TCCTGACATC TATACTTCTC CCCTGATTCC CAAAATGATG 
CCTTCACTGC GGCACACCAA GAGCGAAGAG CACCTGGATG GGTCCTGCCT GAACTCCCAC 
CAACATCTGA GCTGCAGTCG AACTTCAAGC TGCAGTAGCA GTCAGCAAGG CACAAGTTGC 
CGCCGAAAAA AAGGAGCAGA TGTAGATGGC GGCTGCGGGG ATGCGGGTCC ACATAATAGC 
TGCAGCAATG TACATTCGTT AGGTGGACTT CAGTTCCAGG AAGATGACGT GTGGTCACAA 
GAGGAGGACG AAATAACAGC GCAGTATCTT AACATGCGGA AAGTGAGCGA GCGGGACACA 
ATTTCACAAA TATCACTAGA TTCCTGCGAT TCAAGAGATC AAGGTCCACC AGTCGTGTTT 
AATATTGGCG ACGTGCGCTT GCGCCACTGC AGCAATTTGA GCTGCGAAAA TACTAAATCC 
TTCAGTAACG ACCCGGAGGA TCCTTCGGCG GCAGCTTTAA AGGAACCGTG GCACGAAAAG 
GAAAAGCTTA TCCGCGAGTC TTCGCCATAC GGCCATCTAT CAAACTGGCG ACTGCTTTCC 
GCCATCGTAA AGTGTGGCGA TGATTTACGC CAAGAACTAA TGGCTACCCA ACTTTTACAG 
ATGTTTAAAA TTATTTGGCA AGAAGAGCAA GTAGATCTGT GGGTGCGCCC GTACAAAATA 
GTCTGCCTCT CGAATGATAG TGGTCTTATA GAGCCGATCC TTAACACTGT ATCCCTGCAC 
CAAATTAAGA AGAACTCCAA CAAATCGTTG AAAGAGTATT TTATCGATGA ATACGGCTCA 
CCAAGCGGAG AAAGTTTTCG GCGCGCGCAG AAGAACTTCG TCCAAAGCTG TGCAGCTTAC 
TGTCTAATTT CATATTTACT GCAAGTTAAG GACAGGCATA ATGGCAACAT TCTCTTCCAT 
TCGGATGGGC ACATTATACA CATTGACTTT GGATTTATTT TAAGTATATC ACCTAAAAAC 
TTGGGATTCG AACAGTCGCC CTTTAAGCTA ACGCCGGAAT TTGTTGAGGT GATGGGTGGC 
ACGAGCTCGG AGCATTGGCG TGAATTTAAT AAGCTCTTGT TAGTTGGAAT GATGTCTGCT 
CGAAAGCATA TGGATCGTAT TATTAACTTC GTGGAAATTA TGCGCAGCAA TGCGCATTTG 
CCGTGCTTTA AGAATGGGTG CTCTGGAACC GTACAGAATC TCCGAAAACG CTTTCATATG 
AATTTAACCG AACAAGAAAT GGAGCGAAAG GTTGAGCAGC TTGTACAGGA CTCGTTAAAA 
AGTCTGTCAA CCAAACTGTA CGACGGTTAT CAATACTACA CTAATGGCAT ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728519.1
CDS	266..4282
Translation	MGILLPPVSQVTNNHINHTQHHRNRSLDSALQRIPEVEVSSPNAESENTFCSPSILGATMCSKIATTAATVTSALSPPGTPTPALMSTACTPTIGAVKGNVQHPVPKHEQSISLAAACRSSAVVESCPTLRMRPKSSESVAANGQEMIGVIVSSTGNGSSSSNNSCSSAGSTKKREDLTSLGSDDSGIICGSESDQISLNRICHSHESLDSGEMDADADAEEECVDLMDTTSIDEEYNIMQPDHLCLYPPQAASTELDCRTLRPSRKQKRQQQQVLERKNLPSQNQIESEDSVDAGVDARTRYRVMNMSQAAINVELGTASNEIDPDVNYRESHQDPIADAPSLSTPSVASTASATPSVTPTPIATPTPTESTKNDQVLFKNFFGATKNAIFRTAQSIIENHEKKNAAKQKEQPDHSGTVSLVGSPNGGSSVPQDLVKSPTDISKKKEFFSLLTSSSSIKAAAAAAANSAASTPPATPTEALAANSNSKLQPLSDPSADLKVKERTLNLRLPGDSEGSEGALTKCSSINSIHKLKLPVPGVVKYFISEKAPVSDVLQKPEKGQSGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFSFPDQEVDLYIPQLVVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNSHNSSRKSKLALMKEIFSKREHKQTQNDLKSAGTGGLHVGEPRSVVALAKKTHHRSQSDATVLLADFRSPHTLSISHRMYQQPPYTTQTLPTTPAKLCLGDLTSGHAFDNGCTCFETVRGQVNGLLGQRTLCSCGAPKTSPQKEFMKALMNVGKNLTSWPSKAEKTSALRMSLNLINKNLPARVWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPKMMPSLRHTKSEEHLDGSCLNSHQHLSCSRTSSCSSSQQGTSCRRKKGADVDGGCGDAGPHNSCSNVHSLGGLQFQEDDVWSQEEDEITAQYLNMRKVSERDTISQISLDSCDSRDQGPPVVFNIGDVRLRHCSNLSCENTKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKKNSNKSLKEYFIDEYGSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISPKNLGFEQSPFKLTPEFVEVMGGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRSNAHLPCFKNGCSGTVQNLRKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGIL

Target ORF information:

RefSeq Version	NM_167827.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster four wheel drive (fwd), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167827.2

ORF Insert Sequence:

ATGGGCATAC TTCTTCCGCC TGTCTCGCAG GTGACCAATA ATCACATCAA TCACACCCAG 
CACCACCGCA ATCGCAGCCT CGACAGTGCC TTGCAACGCA TTCCGGAGGT CGAAGTGTCA 
TCGCCAAATG CTGAAAGCGA AAACACGTTC TGCTCACCGT CCATTCTCGG TGCGACTATG 
TGCTCAAAAA TTGCCACGAC AGCGGCAACT GTAACGTCAG CCCTGTCCCC ACCAGGCACG 
CCCACACCGG CTCTGATGTC GACGGCGTGC ACGCCCACGA TTGGAGCCGT AAAGGGCAAC 
GTGCAACATC CCGTGCCGAA GCATGAGCAA TCGATTTCTT TGGCAGCTGC TTGCAGGTCC 
AGTGCCGTCG TGGAATCCTG TCCAACGTTA CGAATGCGCC CAAAATCCAG CGAGTCGGTT 
GCGGCCAATG GACAAGAGAT GATCGGCGTC ATTGTCAGCA GCACGGGCAA CGGCAGTAGC 
AGCAGTAATA ATAGCTGTAG TAGCGCTGGT AGCACCAAGA AGCGAGAGGA TCTCACCAGT 
CTTGGATCGG ATGATTCTGG CATCATCTGT GGCTCAGAGT CGGACCAAAT ATCTCTGAAT 
CGCATATGCC ACTCACATGA GTCGCTTGAT TCGGGTGAGA TGGATGCGGA TGCCGATGCA 
GAAGAGGAGT GCGTTGATCT TATGGATACG ACATCCATCG ACGAGGAGTA CAACATTATG 
CAGCCGGACC ATCTCTGCCT CTATCCTCCA CAGGCCGCTT CCACCGAATT GGACTGCCGC 
ACCCTGCGTC CGTCTCGGAA ACAAAAGCGA CAGCAACAGC AGGTCCTGGA GCGGAAGAAT 
CTACCGTCCC AAAACCAGAT CGAAAGCGAA GACTCAGTGG ATGCTGGCGT CGACGCCCGA 
ACACGCTATC GCGTTATGAA CATGTCGCAG GCAGCTATCA ATGTTGAACT CGGAACAGCT 
TCCAATGAAA TCGATCCGGA TGTCAACTAC AGAGAATCTC ACCAAGATCC CATCGCTGAT 
GCACCTTCCC TCTCCACCCC AAGTGTAGCA TCAACGGCGT CAGCCACGCC ATCGGTAACC 
CCAACACCGA TTGCCACTCC GACACCAACG GAGAGCACCA AGAACGACCA AGTGCTGTTT 
AAGAATTTCT TTGGCGCCAC CAAGAATGCC ATATTTCGCA CAGCCCAGAG CATTATTGAA 
AACCATGAGA AGAAAAACGC TGCTAAGCAG AAGGAGCAGC CTGATCACTC AGGGACAGTC 
AGCTTAGTGG GTTCGCCAAA TGGAGGAAGC AGTGTGCCAC AAGACCTGGT CAAGTCTCCC 
ACAGACATTT CGAAAAAGAA GGAATTCTTT AGTTTGCTCA CGTCTAGTTC CAGCATAAAG 
GCAGCGGCTG CAGCTGCGGC GAACTCAGCT GCTAGCACTC CTCCTGCCAC GCCTACGGAG 
GCACTAGCTG CGAATTCAAA TTCGAAGCTT CAACCGCTTT CCGATCCATC TGCCGATTTA 
AAAGTAAAGG AACGTACTCT CAACTTGCGT CTACCTGGAG ATAGTGAAGG CAGTGAAGGA 
GCATTGACAA AGTGTTCATC AATCAACTCG ATACACAAAC TAAAGCTTCC TGTTCCTGGT 
GTCGTTAAGT ATTTCATCAG TGAAAAAGCA CCTGTGTCTG ATGTGCTCCA GAAGCCAGAG 
AAAGGCCAAA GCGGCCTTTT GCGTTTCTTT GAGTCGCCAG TGTTCAACAT ACACTTTGCT 
GTGCATTATC TCTTTTACTC AAAGGAACCG GGAGTCCTCA GCTTTATCGG CAACAAGATC 
TTCAGTTTTC CCGACCAAGA GGTTGATCTG TACATACCAC AATTGGTTGT TATGTACATT 
CAGATGGATG AGCTAGCTGA GGTTCTGGAT CCGTATCTTA CCATTCGGTG TCGCAAGTCG 
GTTGACTTCT CGCTGAAGTG TCTGTGGCTC TTGGAGGCAT ATAACTACCA AGTGGACTCG 
CTAGGGAACT CACACAACAG TTCTCGGAAA TCAAAGCTGG CGCTCATGAA GGAAATATTC 
TCAAAAAGGG AGCACAAACA GACTCAAAAC GACCTCAAGT CTGCAGGTAC TGGAGGTCTG 
CATGTTGGAG AGCCGAGGTC GGTAGTCGCG CTTGCCAAGA AGACTCACCA CCGCTCGCAA 
TCTGATGCCA CTGTGCTCCT AGCCGACTTT CGCTCGCCTC ACACACTGAG TATATCGCAT 
CGTATGTACC AGCAGCCACC CTATACCACA CAAACACTTC CCACTACGCC CGCCAAGCTG 
TGCCTCGGTG ACTTAACTTC CGGCCACGCA TTTGATAACG GGTGCACCTG CTTCGAGACG 
GTTCGAGGCC AAGTAAATGG TCTGCTTGGG CAGAGGACGC TATGCAGCTG TGGTGCTCCA 
AAGACGTCAC CCCAGAAAGA GTTTATGAAG GCATTGATGA ACGTTGGTAA AAATCTCACC 
TCGTGGCCTT CCAAAGCGGA GAAGACTTCA GCGCTGCGGA TGTCCCTCAA CTTGATTAAC 
AAAAACCTGC CCGCTAGAGT CTGGCTTCCT TTGTACTCAG ACATTCCACA TCATGTCGTT 
CGTATTACAG AGGAGAAGAC CGCAGTACTG AACTCGAAGG ATAAAACCCC CTATATCATA 
TACGTAGAAG TTGTTGAAAT TCCTGACATC TATACTTCTC CCCTGATTCC CAAAATGATG 
CCTTCACTGC GGCACACCAA GAGCGAAGAG CACCTGGATG GGTCCTGCCT GAACTCCCAC 
CAACATCTGA GCTGCAGTCG AACTTCAAGC TGCAGTAGCA GTCAGCAAGG CACAAGTTGC 
CGCCGAAAAA AAGGAGCAGA TGTAGATGGC GGCTGCGGGG ATGCGGGTCC ACATAATAGC 
TGCAGCAATG TACATTCGTT AGGTGGACTT CAGTTCCAGG AAGATGACGT GTGGTCACAA 
GAGGAGGACG AAATAACAGC GCAGTATCTT AACATGCGGA AAGTGAGCGA GCGGGACACA 
ATTTCACAAA TATCACTAGA TTCCTGCGAT TCAAGAGATC AAGGTCCACC AGTCGTGTTT 
AATATTGGCG ACGTGCGCTT GCGCCACTGC AGCAATTTGA GCTGCGAAAA TACTAAATCC 
TTCAGTAACG ACCCGGAGGA TCCTTCGGCG GCAGCTTTAA AGGAACCGTG GCACGAAAAG 
GAAAAGCTTA TCCGCGAGTC TTCGCCATAC GGCCATCTAT CAAACTGGCG ACTGCTTTCC 
GCCATCGTAA AGTGTGGCGA TGATTTACGC CAAGAACTAA TGGCTACCCA ACTTTTACAG 
ATGTTTAAAA TTATTTGGCA AGAAGAGCAA GTAGATCTGT GGGTGCGCCC GTACAAAATA 
GTCTGCCTCT CGAATGATAG TGGTCTTATA GAGCCGATCC TTAACACTGT ATCCCTGCAC 
CAAATTAAGA AGAACTCCAA CAAATCGTTG AAAGAGTATT TTATCGATGA ATACGGCTCA 
CCAAGCGGAG AAAGTTTTCG GCGCGCGCAG AAGAACTTCG TCCAAAGCTG TGCAGCTTAC 
TGTCTAATTT CATATTTACT GCAAGTTAAG GACAGGCATA ATGGCAACAT TCTCTTCCAT 
TCGGATGGGC ACATTATACA CATTGACTTT GGATTTATTT TAAGTATATC ACCTAAAAAC 
TTGGGATTCG AACAGTCGCC CTTTAAGCTA ACGCCGGAAT TTGTTGAGGT GATGGGTGGC 
ACGAGCTCGG AGCATTGGCG TGAATTTAAT AAGCTCTTGT TAGTTGGAAT GATGTCTGCT 
CGAAAGCATA TGGATCGTAT TATTAACTTC GTGGAAATTA TGCGCAGCAA TGCGCATTTG 
CCGTGCTTTA AGAATGGGTG CTCTGGAACC GTACAGAATC TCCGAAAACG CTTTCATATG 
AATTTAACCG AACAAGAAAT GGAGCGAAAG GTTGAGCAGC TTGTACAGGA CTCGTTAAAA 
AGTCTGTCAA CCAAACTGTA CGACGGTTAT CAATACTACA CTAATGGCAT ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fwd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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