pk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pk
Entrez Gene ID	45343
Full Name	prickle
Synonyms	CG11084,CG12830,Dmel\CG11084,Pk,Pk-Sple,Sple,pk-sple,pkM,sple
General protein information	Preferred Names prickle Names CG11084 gene product from transcript CG11084-RA CG11084-PA CG11084-PB CG11084-PC pk-PA pk-PB pk-PC prickle-spiny legs prickle-spiny-legs spiny legs
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42F3-43A1\|2-55.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_165512.2	NP_724538.1	prickle, isoform C
NM_165509.2	NP_724535.1	prickle, isoform B
NM_165508.2	NP_724534.1	prickle, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We find that the E3 ubiquitin ligase complex Cullin1(Cul1)/SkpA/Supernumerary limbs(Slimb) regulates the stability of one of the peripheral membrane components, Prickle (Pk).
Title: Clustering and negative feedback by endocytosis in planar cell polarity signaling is modulated by ubiquitinylation of prickle.

Fat and Core planar cell polarity (PCP) pathways patterns change during morphogenesis, undergoing phases of coupling and uncoupling that are regulated by antagonistic Core PCP protein isoforms Prickle and Spiny-legs.
Title: The balance of prickle/spiny-legs isoforms controls the amount of coupling between core and fat PCP systems.

These data reveal a previously unidentified pathway in the pathophysiology of seizure disorders and provide evidence for a more generalized cellular mechanism whereby Prickle mediates polarity by influencing microtubule-mediated transport.
Title: prickle modulates microtubule polarity and axonal transport to ameliorate seizures in flies.

Alternative Prickle (Pk-Sple) protein isoforms control the polarity of microtubule network.
Title: Prickle/spiny-legs isoforms control the polarity of the apical microtubule network in planar cell polarity.

Pk and Sple form different complexes with distinct subcellular localizations in the eye and wing.
Title: Dachsous-dependent asymmetric localization of spiny-legs determines planar cell polarity orientation in Drosophila.

The following pk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11396	NM_165508.2 Latest version!	Drosophila melanogaster prickle (pk), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa11397	NM_165509.2 Latest version!	Drosophila melanogaster prickle (pk), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa11398	NM_165512.2 Latest version!	Drosophila melanogaster prickle (pk), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pk ( NM_165508.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724534.1 Drosophila melanogaster prickle (pk), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11396
Clone ID Related Accession (Same CDS sequence)	NM_165508.2
Accession Version	NM_165508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	prickle, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165508.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATACCC CAAATCAAAT GCCTGTTGAG CTAGAAAGGC CCATCAGCCG AACTCCGCTA 
ACGCAGATCT CCTATCTGCA GAAGATCCCC ACGCTTCCGC GCCACTTCTC GCCCAGTGGG 
CAGGGCCTGG CCACGCCCCC AGCCCTCGGC AGCGGCGGCA TGGGTCTGCC CAGCAGCTCC 
TCCGCCAGTG CCCTCTACGC GGCCCAGGCA GCAGCCGGAA TCCTGCCCAC CTCCCCGCTG 
CCACTGCAGC GCCACCAGCA GTACTTGCCG CCACATCACC AACAGCACCC GGGAGCTGGA 
ATGGGACCAG GACCAGGATC GGGGGCAGCA GCAGGACCGC CCCTTGGCCC CCAGTACTCG 
CCAGGCTGCT CCGCCAATCC CAAGTACTCG AATGCCCAGC TCCCGCCACC GCCGCACCAC 
CACCACCAGC TATCGCCGGC TTTGTCGACG CCCTCGCCAC CCTCGCTGCT CCACCATCCC 
GCCGGAGGCA CTTCCTCTGC CTCGGCCCAC GCCCCCTTCC TGGGCGGACC GCACATGGAC 
ATGCAGCGGC AGTCGCACTC GGACGACGAC AGTGGCTGTG CTCTGGAGGA GTACACCTGG 
GTGCCACCCG GACTGCGGCC TGATCAGGTT CGCTTGTACT TCTCGCAGAT ACCCGACGAC 
AAAGTGCCAT ACGTCAACAG TCCGGGGGAG CAGTATCGTG TGCGACAATT GCTCCATCAA 
CTTCCGCCGC ATGATAACGA GGTTCGTTAC TGTCACTCAC TGACGGATGA GGAGCGCAAG 
GAGCTGCGGT TATTCTCCAC GCAGCGCAAA CGCGATGCCC TGGGCCGCGG CAACGTGCGG 
CAGCTGATGA GCGCCCGACC CTGCGACGGC TGCGACGACC TAATCTCCAC GGGCGACATC 
GCCGTGTTCG CCACGCGACT GGGCCCCAAT GCCAGCTGGC ATCCGGCGTG CTTCGCCTGC 
TCCGTCTGCC GCGAGCTCCT CGTGGACCTT ATCTACTTCC ACCGGGATGG GCGCATGTAC 
TGCGGACGCC ACCACGCCGA GACCCTAAAG CCCCGCTGCT CGGCCTGCGA TGAGATTATC 
CTGGCAGACG AGTGCACGGA GGCGGAGGGC AGGGCGTGGC ACATGAACCA CTTTGCCTGC 
CACGAGTGCG ACAAGCAGCT GGGCGGCCAG CGGTATATCA TGCGGGAGGG CAAACCCTAC 
TGTCTGCACT GCTTCGACGC GATGTTTGCC GAGTACTGCG ACTACTGCGG GGAGGCCATC 
GGGGTGGACC AGGGCCAGAT GAGCCACGAC GGGCAACACT GGCACGCCAC CGACGAGTGC 
TTCTCCTGCA ACACTTGCCG CTGCTCGCTT CTGGGACGCG CCTTCCTGCC GCGACGAGGG 
GCCATCTACT GCTCCATCGC CTGCAGCAAA GGAGAGCCGC CCACGCCGTC GGACAGCTCC 
GGCACCGGCA TGTACACCAC GCCAACTCCG CCCACACAGC GGGTGCGGCC CCATCCGCAA 
GCGCCTCTGC CGGCGCGCAT TCCCAGCAGC CACGCCTCCA GCTCGCCGCC TATGTCGCCG 
CAACAGCAGC AGCAGCACCA GGCTACCTTC AACCAAGCGA TGTACCAGAT GCAAAGCCAG 
CAGATGGAGG CGGCGGGCGG ACTGGTGGAC CAGAGCAAGA GCTACGCCGC GTCCGACTCG 
GATGCGGGTG TGGTAAAGGA CCTAGAACAC GGCGGACATA TGGGCGGAGG AGATCTGACG 
GACTTTTCGG GTGGACGGGC CTCCAGCACC TCGCAGAACC TATCGCCTCT GAACTCACCC 
GGCGACTTCC AGCCCCATTT TCTGCCCAAG CCCATGGAAC TGCAGCGCGA CGGAGTGTAC 
AACTTCAACG AGATGTCCTC TAATCTCGAT GCAGCCTGGT CAGCGAAGCC CACAAACAGT 
TACCATCTGC AGAGACAGCT CCTGGAGAAC CCCCACACCG CCAGCATGCC GGAACTGGCG 
GGTAAACTGG TGGCGCCTCC TGCACACATG CAGCACCTGT CCCAGCTGCA CGCCGTGTCC 
TCGCACCAGT TCCAGCAGCA CGAGTACGCG GACATCCTGC ATCCGCCGCC TCCTCCGCCT 
GGTGAGATTC CCGAGCTGCC CACTCCCAAC TTGAGCGTGG CCTCCACCGC CTTGCCGCCA 
GAGCTGATGG GCTCACCCAC CCACTCGGCG GGCGACAGGT CGCTGAACAC GCCCATGTCC 
ACACAGTCGG CCTCCCACGC GCCCCCACAT CCGGTCTCCA TACTAAGCGG CGCCTCCTCC 
TCCTCGCCGA TGAGCGGGGA GCCGGCCAAG AAGAAGGGCG TGCGCTTCGA GGGAATTCCG 
GACACACTGC CCCGATCACG CAGCTACTCC GGTAACGGAG CGGGAACGAG TGGCGGCGGC 
GAGAGGGAGC GGGATCGGGA CAAGGACAAG GAGGGAGGAG GTCGCCATGG TCACGGTCAC 
TCCTCGCGAA GGCGAAGACG TCGCAAGTCC TCCTCCAGCT CGTCGCACCA TCGCAGCGGC 
AGCGGTCATC GCTCCCACTC GACCACTCGA GCGGACACCT ATGCACCTGC GCAACCCCTA 
TCCTCGTCCT ACCAGGGTCC ACCTTCGGTT CTGCAGGCTG CCAACCTGGT CCACGAGTCA 
CCCAGCCGAC AGCAGAGGGA ACGGGAGCGC GAGCGGGAAC GCGAGGAGTC CGAGGAGTCT 
GACGTGTGCT CCACCTGCTC CTCGAGCTCC TCCAGCTCGG AGGACTACAT GATGATGTAC 
CAGCTGCCGC AGAGGCGCCA CTACGGCGGC GTGCGCGTGA GCTATGTGCC CAACGATGCA 
CTGGCCTACG ATCGGAAGAG GAAGCCCAGT GAACTGGGCG GCGACAAGGA CAAGAACTGC 
ATCATCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724534.1
CDS	791..3682
Translation	MDTPNQMPVELERPISRTPLTQISYLQKIPTLPRHFSPSGQGLATPPALGSGGMGLPSSSSASALYAAQAAAGILPTSPLPLQRHQQYLPPHHQQHPGAGMGPGPGSGAAAGPPLGPQYSPGCSANPKYSNAQLPPPPHHHHQLSPALSTPSPPSLLHHPAGGTSSASAHAPFLGGPHMDMQRQSHSDDDSGCALEEYTWVPPGLRPDQVRLYFSQIPDDKVPYVNSPGEQYRVRQLLHQLPPHDNEVRYCHSLTDEERKELRLFSTQRKRDALGRGNVRQLMSARPCDGCDDLISTGDIAVFATRLGPNASWHPACFACSVCRELLVDLIYFHRDGRMYCGRHHAETLKPRCSACDEIILADECTEAEGRAWHMNHFACHECDKQLGGQRYIMREGKPYCLHCFDAMFAEYCDYCGEAIGVDQGQMSHDGQHWHATDECFSCNTCRCSLLGRAFLPRRGAIYCSIACSKGEPPTPSDSSGTGMYTTPTPPTQRVRPHPQAPLPARIPSSHASSSPPMSPQQQQQHQATFNQAMYQMQSQQMEAAGGLVDQSKSYAASDSDAGVVKDLEHGGHMGGGDLTDFSGGRASSTSQNLSPLNSPGDFQPHFLPKPMELQRDGVYNFNEMSSNLDAAWSAKPTNSYHLQRQLLENPHTASMPELAGKLVAPPAHMQHLSQLHAVSSHQFQQHEYADILHPPPPPPGEIPELPTPNLSVASTALPPELMGSPTHSAGDRSLNTPMSTQSASHAPPHPVSILSGASSSSPMSGEPAKKKGVRFEGIPDTLPRSRSYSGNGAGTSGGGERERDRDKDKEGGGRHGHGHSSRRRRRRKSSSSSSHHRSGSGHRSHSTTRADTYAPAQPLSSSYQGPPSVLQAANLVHESPSRQQREREREREREESEESDVCSTCSSSSSSSEDYMMMYQLPQRRHYGGVRVSYVPNDALAYDRKRKPSELGGDKDKNCIIS

Target ORF information:

RefSeq Version	NM_165508.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster prickle (pk), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165508.2

ORF Insert Sequence:

ATGGATACCC CAAATCAAAT GCCTGTTGAG CTAGAAAGGC CCATCAGCCG AACTCCGCTA 
ACGCAGATCT CCTATCTGCA GAAGATCCCC ACGCTTCCGC GCCACTTCTC GCCCAGTGGG 
CAGGGCCTGG CCACGCCCCC AGCCCTCGGC AGCGGCGGCA TGGGTCTGCC CAGCAGCTCC 
TCCGCCAGTG CCCTCTACGC GGCCCAGGCA GCAGCCGGAA TCCTGCCCAC CTCCCCGCTG 
CCACTGCAGC GCCACCAGCA GTACTTGCCG CCACATCACC AACAGCACCC GGGAGCTGGA 
ATGGGACCAG GACCAGGATC GGGGGCAGCA GCAGGACCGC CCCTTGGCCC CCAGTACTCG 
CCAGGCTGCT CCGCCAATCC CAAGTACTCG AATGCCCAGC TCCCGCCACC GCCGCACCAC 
CACCACCAGC TATCGCCGGC TTTGTCGACG CCCTCGCCAC CCTCGCTGCT CCACCATCCC 
GCCGGAGGCA CTTCCTCTGC CTCGGCCCAC GCCCCCTTCC TGGGCGGACC GCACATGGAC 
ATGCAGCGGC AGTCGCACTC GGACGACGAC AGTGGCTGTG CTCTGGAGGA GTACACCTGG 
GTGCCACCCG GACTGCGGCC TGATCAGGTT CGCTTGTACT TCTCGCAGAT ACCCGACGAC 
AAAGTGCCAT ACGTCAACAG TCCGGGGGAG CAGTATCGTG TGCGACAATT GCTCCATCAA 
CTTCCGCCGC ATGATAACGA GGTTCGTTAC TGTCACTCAC TGACGGATGA GGAGCGCAAG 
GAGCTGCGGT TATTCTCCAC GCAGCGCAAA CGCGATGCCC TGGGCCGCGG CAACGTGCGG 
CAGCTGATGA GCGCCCGACC CTGCGACGGC TGCGACGACC TAATCTCCAC GGGCGACATC 
GCCGTGTTCG CCACGCGACT GGGCCCCAAT GCCAGCTGGC ATCCGGCGTG CTTCGCCTGC 
TCCGTCTGCC GCGAGCTCCT CGTGGACCTT ATCTACTTCC ACCGGGATGG GCGCATGTAC 
TGCGGACGCC ACCACGCCGA GACCCTAAAG CCCCGCTGCT CGGCCTGCGA TGAGATTATC 
CTGGCAGACG AGTGCACGGA GGCGGAGGGC AGGGCGTGGC ACATGAACCA CTTTGCCTGC 
CACGAGTGCG ACAAGCAGCT GGGCGGCCAG CGGTATATCA TGCGGGAGGG CAAACCCTAC 
TGTCTGCACT GCTTCGACGC GATGTTTGCC GAGTACTGCG ACTACTGCGG GGAGGCCATC 
GGGGTGGACC AGGGCCAGAT GAGCCACGAC GGGCAACACT GGCACGCCAC CGACGAGTGC 
TTCTCCTGCA ACACTTGCCG CTGCTCGCTT CTGGGACGCG CCTTCCTGCC GCGACGAGGG 
GCCATCTACT GCTCCATCGC CTGCAGCAAA GGAGAGCCGC CCACGCCGTC GGACAGCTCC 
GGCACCGGCA TGTACACCAC GCCAACTCCG CCCACACAGC GGGTGCGGCC CCATCCGCAA 
GCGCCTCTGC CGGCGCGCAT TCCCAGCAGC CACGCCTCCA GCTCGCCGCC TATGTCGCCG 
CAACAGCAGC AGCAGCACCA GGCTACCTTC AACCAAGCGA TGTACCAGAT GCAAAGCCAG 
CAGATGGAGG CGGCGGGCGG ACTGGTGGAC CAGAGCAAGA GCTACGCCGC GTCCGACTCG 
GATGCGGGTG TGGTAAAGGA CCTAGAACAC GGCGGACATA TGGGCGGAGG AGATCTGACG 
GACTTTTCGG GTGGACGGGC CTCCAGCACC TCGCAGAACC TATCGCCTCT GAACTCACCC 
GGCGACTTCC AGCCCCATTT TCTGCCCAAG CCCATGGAAC TGCAGCGCGA CGGAGTGTAC 
AACTTCAACG AGATGTCCTC TAATCTCGAT GCAGCCTGGT CAGCGAAGCC CACAAACAGT 
TACCATCTGC AGAGACAGCT CCTGGAGAAC CCCCACACCG CCAGCATGCC GGAACTGGCG 
GGTAAACTGG TGGCGCCTCC TGCACACATG CAGCACCTGT CCCAGCTGCA CGCCGTGTCC 
TCGCACCAGT TCCAGCAGCA CGAGTACGCG GACATCCTGC ATCCGCCGCC TCCTCCGCCT 
GGTGAGATTC CCGAGCTGCC CACTCCCAAC TTGAGCGTGG CCTCCACCGC CTTGCCGCCA 
GAGCTGATGG GCTCACCCAC CCACTCGGCG GGCGACAGGT CGCTGAACAC GCCCATGTCC 
ACACAGTCGG CCTCCCACGC GCCCCCACAT CCGGTCTCCA TACTAAGCGG CGCCTCCTCC 
TCCTCGCCGA TGAGCGGGGA GCCGGCCAAG AAGAAGGGCG TGCGCTTCGA GGGAATTCCG 
GACACACTGC CCCGATCACG CAGCTACTCC GGTAACGGAG CGGGAACGAG TGGCGGCGGC 
GAGAGGGAGC GGGATCGGGA CAAGGACAAG GAGGGAGGAG GTCGCCATGG TCACGGTCAC 
TCCTCGCGAA GGCGAAGACG TCGCAAGTCC TCCTCCAGCT CGTCGCACCA TCGCAGCGGC 
AGCGGTCATC GCTCCCACTC GACCACTCGA GCGGACACCT ATGCACCTGC GCAACCCCTA 
TCCTCGTCCT ACCAGGGTCC ACCTTCGGTT CTGCAGGCTG CCAACCTGGT CCACGAGTCA 
CCCAGCCGAC AGCAGAGGGA ACGGGAGCGC GAGCGGGAAC GCGAGGAGTC CGAGGAGTCT 
GACGTGTGCT CCACCTGCTC CTCGAGCTCC TCCAGCTCGG AGGACTACAT GATGATGTAC 
CAGCTGCCGC AGAGGCGCCA CTACGGCGGC GTGCGCGTGA GCTATGTGCC CAACGATGCA 
CTGGCCTACG ATCGGAAGAG GAAGCCCAGT GAACTGGGCG GCGACAAGGA CAAGAACTGC 
ATCATCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pk ( NM_165509.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724535.1 Drosophila melanogaster prickle (pk), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11397
Clone ID Related Accession (Same CDS sequence)	NM_165509.2
Accession Version	NM_165509.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	prickle, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165509.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGATT CGACCGATAA TTTGCATGCC GACTGCGACG GTCGAGTCAG CAACAACAAC 
AATGGCAACA GCAACACCAA CGATGGACCC AACAACGACG GCGACTCCGA CGAGGAGGTC 
ATCGAGGGCA TGGCGCTGCT GGAGGGCAAC TACCAAGTGC TGCGCCAGTG GGTGCCGCCC 
GCCCCCAACT ATTGGGATGC GCCCCCCAAG GCCATCATCA AATCCGCCGA AGTCAGGCCC 
ATCAGCCGAA CTCCGCTAAC GCAGATCTCC TATCTGCAGA AGATCCCCAC GCTTCCGCGC 
CACTTCTCGC CCAGTGGGCA GGGCCTGGCC ACGCCCCCAG CCCTCGGCAG CGGCGGCATG 
GGTCTGCCCA GCAGCTCCTC CGCCAGTGCC CTCTACGCGG CCCAGGCAGC AGCCGGAATC 
CTGCCCACCT CCCCGCTGCC ACTGCAGCGC CACCAGCAGT ACTTGCCGCC ACATCACCAA 
CAGCACCCGG GAGCTGGAAT GGGACCAGGA CCAGGATCGG GGGCAGCAGC AGGACCGCCC 
CTTGGCCCCC AGTACTCGCC AGGCTGCTCC GCCAATCCCA AGTACTCGAA TGCCCAGCTC 
CCGCCACCGC CGCACCACCA CCACCAGCTA TCGCCGGCTT TGTCGACGCC CTCGCCACCC 
TCGCTGCTCC ACCATCCCGC CGGAGGCACT TCCTCTGCCT CGGCCCACGC CCCCTTCCTG 
GGCGGACCGC ACATGGACAT GCAGCGGCAG TCGCACTCGG ACGACGACAG TGGCTGTGCT 
CTGGAGGAGT ACACCTGGGT GCCACCCGGA CTGCGGCCTG ATCAGGTTCG CTTGTACTTC 
TCGCAGATAC CCGACGACAA AGTGCCATAC GTCAACAGTC CGGGGGAGCA GTATCGTGTG 
CGACAATTGC TCCATCAACT TCCGCCGCAT GATAACGAGG TTCGTTACTG TCACTCACTG 
ACGGATGAGG AGCGCAAGGA GCTGCGGTTA TTCTCCACGC AGCGCAAACG CGATGCCCTG 
GGCCGCGGCA ACGTGCGGCA GCTGATGAGC GCCCGACCCT GCGACGGCTG CGACGACCTA 
ATCTCCACGG GCGACATCGC CGTGTTCGCC ACGCGACTGG GCCCCAATGC CAGCTGGCAT 
CCGGCGTGCT TCGCCTGCTC CGTCTGCCGC GAGCTCCTCG TGGACCTTAT CTACTTCCAC 
CGGGATGGGC GCATGTACTG CGGACGCCAC CACGCCGAGA CCCTAAAGCC CCGCTGCTCG 
GCCTGCGATG AGATTATCCT GGCAGACGAG TGCACGGAGG CGGAGGGCAG GGCGTGGCAC 
ATGAACCACT TTGCCTGCCA CGAGTGCGAC AAGCAGCTGG GCGGCCAGCG GTATATCATG 
CGGGAGGGCA AACCCTACTG TCTGCACTGC TTCGACGCGA TGTTTGCCGA GTACTGCGAC 
TACTGCGGGG AGGCCATCGG GGTGGACCAG GGCCAGATGA GCCACGACGG GCAACACTGG 
CACGCCACCG ACGAGTGCTT CTCCTGCAAC ACTTGCCGCT GCTCGCTTCT GGGACGCGCC 
TTCCTGCCGC GACGAGGGGC CATCTACTGC TCCATCGCCT GCAGCAAAGG AGAGCCGCCC 
ACGCCGTCGG ACAGCTCCGG CACCGGCATG TACACCACGC CAACTCCGCC CACACAGCGG 
GTGCGGCCCC ATCCGCAAGC GCCTCTGCCG GCGCGCATTC CCAGCAGCCA CGCCTCCAGC 
TCGCCGCCTA TGTCGCCGCA ACAGCAGCAG CAGCACCAGG CTACCTTCAA CCAAGCGATG 
TACCAGATGC AAAGCCAGCA GATGGAGGCG GCGGGCGGAC TGGTGGACCA GAGCAAGAGC 
TACGCCGCGT CCGACTCGGA TGCGGGTGTG GTAAAGGACC TAGAACACGG CGGACATATG 
GGCGGAGGAG ATCTGACGGA CTTTTCGGGT GGACGGGCCT CCAGCACCTC GCAGAACCTA 
TCGCCTCTGA ACTCACCCGG CGACTTCCAG CCCCATTTTC TGCCCAAGCC CATGGAACTG 
CAGCGCGACG GAGTGTACAA CTTCAACGAG ATGTCCTCTA ATCTCGATGC AGCCTGGTCA 
GCGAAGCCCA CAAACAGTTA CCATCTGCAG AGACAGCTCC TGGAGAACCC CCACACCGCC 
AGCATGCCGG AACTGGCGGG TAAACTGGTG GCGCCTCCTG CACACATGCA GCACCTGTCC 
CAGCTGCACG CCGTGTCCTC GCACCAGTTC CAGCAGCACG AGTACGCGGA CATCCTGCAT 
CCGCCGCCTC CTCCGCCTGG TGAGATTCCC GAGCTGCCCA CTCCCAACTT GAGCGTGGCC 
TCCACCGCCT TGCCGCCAGA GCTGATGGGC TCACCCACCC ACTCGGCGGG CGACAGGTCG 
CTGAACACGC CCATGTCCAC ACAGTCGGCC TCCCACGCGC CCCCACATCC GGTCTCCATA 
CTAAGCGGCG CCTCCTCCTC CTCGCCGATG AGCGGGGAGC CGGCCAAGAA GAAGGGCGTG 
CGCTTCGAGG GAATTCCGGA CACACTGCCC CGATCACGCA GCTACTCCGG TAACGGAGCG 
GGAACGAGTG GCGGCGGCGA GAGGGAGCGG GATCGGGACA AGGACAAGGA GGGAGGAGGT 
CGCCATGGTC ACGGTCACTC CTCGCGAAGG CGAAGACGTC GCAAGTCCTC CTCCAGCTCG 
TCGCACCATC GCAGCGGCAG CGGTCATCGC TCCCACTCGA CCACTCGAGC GGACACCTAT 
GCACCTGCGC AACCCCTATC CTCGTCCTAC CAGGGTCCAC CTTCGGTTCT GCAGGCTGCC 
AACCTGGTCC ACGAGTCACC CAGCCGACAG CAGAGGGAAC GGGAGCGCGA GCGGGAACGC 
GAGGAGTCCG AGGAGTCTGA CGTGTGCTCC ACCTGCTCCT CGAGCTCCTC CAGCTCGGAG 
GACTACATGA TGATGTACCA GCTGCCGCAG AGGCGCCACT ACGGCGGCGT GCGCGTGAGC 
TATGTGCCCA ACGATGCACT GGCCTACGAT CGGAAGAGGA AGCCCAGTGA ACTGGGCGGC 
GACAAGGACA AGAACTGCAT CATCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724535.1
CDS	461..3550
Translation	MNDSTDNLHADCDGRVSNNNNGNSNTNDGPNNDGDSDEEVIEGMALLEGNYQVLRQWVPPAPNYWDAPPKAIIKSAEVRPISRTPLTQISYLQKIPTLPRHFSPSGQGLATPPALGSGGMGLPSSSSASALYAAQAAAGILPTSPLPLQRHQQYLPPHHQQHPGAGMGPGPGSGAAAGPPLGPQYSPGCSANPKYSNAQLPPPPHHHHQLSPALSTPSPPSLLHHPAGGTSSASAHAPFLGGPHMDMQRQSHSDDDSGCALEEYTWVPPGLRPDQVRLYFSQIPDDKVPYVNSPGEQYRVRQLLHQLPPHDNEVRYCHSLTDEERKELRLFSTQRKRDALGRGNVRQLMSARPCDGCDDLISTGDIAVFATRLGPNASWHPACFACSVCRELLVDLIYFHRDGRMYCGRHHAETLKPRCSACDEIILADECTEAEGRAWHMNHFACHECDKQLGGQRYIMREGKPYCLHCFDAMFAEYCDYCGEAIGVDQGQMSHDGQHWHATDECFSCNTCRCSLLGRAFLPRRGAIYCSIACSKGEPPTPSDSSGTGMYTTPTPPTQRVRPHPQAPLPARIPSSHASSSPPMSPQQQQQHQATFNQAMYQMQSQQMEAAGGLVDQSKSYAASDSDAGVVKDLEHGGHMGGGDLTDFSGGRASSTSQNLSPLNSPGDFQPHFLPKPMELQRDGVYNFNEMSSNLDAAWSAKPTNSYHLQRQLLENPHTASMPELAGKLVAPPAHMQHLSQLHAVSSHQFQQHEYADILHPPPPPPGEIPELPTPNLSVASTALPPELMGSPTHSAGDRSLNTPMSTQSASHAPPHPVSILSGASSSSPMSGEPAKKKGVRFEGIPDTLPRSRSYSGNGAGTSGGGERERDRDKDKEGGGRHGHGHSSRRRRRRKSSSSSSHHRSGSGHRSHSTTRADTYAPAQPLSSSYQGPPSVLQAANLVHESPSRQQREREREREREESEESDVCSTCSSSSSSSEDYMMMYQLPQRRHYGGVRVSYVPNDALAYDRKRKPSELGGDKDKNCIIS

Target ORF information:

RefSeq Version	NM_165509.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster prickle (pk), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165509.2

ORF Insert Sequence:

ATGAACGATT CGACCGATAA TTTGCATGCC GACTGCGACG GTCGAGTCAG CAACAACAAC 
AATGGCAACA GCAACACCAA CGATGGACCC AACAACGACG GCGACTCCGA CGAGGAGGTC 
ATCGAGGGCA TGGCGCTGCT GGAGGGCAAC TACCAAGTGC TGCGCCAGTG GGTGCCGCCC 
GCCCCCAACT ATTGGGATGC GCCCCCCAAG GCCATCATCA AATCCGCCGA AGTCAGGCCC 
ATCAGCCGAA CTCCGCTAAC GCAGATCTCC TATCTGCAGA AGATCCCCAC GCTTCCGCGC 
CACTTCTCGC CCAGTGGGCA GGGCCTGGCC ACGCCCCCAG CCCTCGGCAG CGGCGGCATG 
GGTCTGCCCA GCAGCTCCTC CGCCAGTGCC CTCTACGCGG CCCAGGCAGC AGCCGGAATC 
CTGCCCACCT CCCCGCTGCC ACTGCAGCGC CACCAGCAGT ACTTGCCGCC ACATCACCAA 
CAGCACCCGG GAGCTGGAAT GGGACCAGGA CCAGGATCGG GGGCAGCAGC AGGACCGCCC 
CTTGGCCCCC AGTACTCGCC AGGCTGCTCC GCCAATCCCA AGTACTCGAA TGCCCAGCTC 
CCGCCACCGC CGCACCACCA CCACCAGCTA TCGCCGGCTT TGTCGACGCC CTCGCCACCC 
TCGCTGCTCC ACCATCCCGC CGGAGGCACT TCCTCTGCCT CGGCCCACGC CCCCTTCCTG 
GGCGGACCGC ACATGGACAT GCAGCGGCAG TCGCACTCGG ACGACGACAG TGGCTGTGCT 
CTGGAGGAGT ACACCTGGGT GCCACCCGGA CTGCGGCCTG ATCAGGTTCG CTTGTACTTC 
TCGCAGATAC CCGACGACAA AGTGCCATAC GTCAACAGTC CGGGGGAGCA GTATCGTGTG 
CGACAATTGC TCCATCAACT TCCGCCGCAT GATAACGAGG TTCGTTACTG TCACTCACTG 
ACGGATGAGG AGCGCAAGGA GCTGCGGTTA TTCTCCACGC AGCGCAAACG CGATGCCCTG 
GGCCGCGGCA ACGTGCGGCA GCTGATGAGC GCCCGACCCT GCGACGGCTG CGACGACCTA 
ATCTCCACGG GCGACATCGC CGTGTTCGCC ACGCGACTGG GCCCCAATGC CAGCTGGCAT 
CCGGCGTGCT TCGCCTGCTC CGTCTGCCGC GAGCTCCTCG TGGACCTTAT CTACTTCCAC 
CGGGATGGGC GCATGTACTG CGGACGCCAC CACGCCGAGA CCCTAAAGCC CCGCTGCTCG 
GCCTGCGATG AGATTATCCT GGCAGACGAG TGCACGGAGG CGGAGGGCAG GGCGTGGCAC 
ATGAACCACT TTGCCTGCCA CGAGTGCGAC AAGCAGCTGG GCGGCCAGCG GTATATCATG 
CGGGAGGGCA AACCCTACTG TCTGCACTGC TTCGACGCGA TGTTTGCCGA GTACTGCGAC 
TACTGCGGGG AGGCCATCGG GGTGGACCAG GGCCAGATGA GCCACGACGG GCAACACTGG 
CACGCCACCG ACGAGTGCTT CTCCTGCAAC ACTTGCCGCT GCTCGCTTCT GGGACGCGCC 
TTCCTGCCGC GACGAGGGGC CATCTACTGC TCCATCGCCT GCAGCAAAGG AGAGCCGCCC 
ACGCCGTCGG ACAGCTCCGG CACCGGCATG TACACCACGC CAACTCCGCC CACACAGCGG 
GTGCGGCCCC ATCCGCAAGC GCCTCTGCCG GCGCGCATTC CCAGCAGCCA CGCCTCCAGC 
TCGCCGCCTA TGTCGCCGCA ACAGCAGCAG CAGCACCAGG CTACCTTCAA CCAAGCGATG 
TACCAGATGC AAAGCCAGCA GATGGAGGCG GCGGGCGGAC TGGTGGACCA GAGCAAGAGC 
TACGCCGCGT CCGACTCGGA TGCGGGTGTG GTAAAGGACC TAGAACACGG CGGACATATG 
GGCGGAGGAG ATCTGACGGA CTTTTCGGGT GGACGGGCCT CCAGCACCTC GCAGAACCTA 
TCGCCTCTGA ACTCACCCGG CGACTTCCAG CCCCATTTTC TGCCCAAGCC CATGGAACTG 
CAGCGCGACG GAGTGTACAA CTTCAACGAG ATGTCCTCTA ATCTCGATGC AGCCTGGTCA 
GCGAAGCCCA CAAACAGTTA CCATCTGCAG AGACAGCTCC TGGAGAACCC CCACACCGCC 
AGCATGCCGG AACTGGCGGG TAAACTGGTG GCGCCTCCTG CACACATGCA GCACCTGTCC 
CAGCTGCACG CCGTGTCCTC GCACCAGTTC CAGCAGCACG AGTACGCGGA CATCCTGCAT 
CCGCCGCCTC CTCCGCCTGG TGAGATTCCC GAGCTGCCCA CTCCCAACTT GAGCGTGGCC 
TCCACCGCCT TGCCGCCAGA GCTGATGGGC TCACCCACCC ACTCGGCGGG CGACAGGTCG 
CTGAACACGC CCATGTCCAC ACAGTCGGCC TCCCACGCGC CCCCACATCC GGTCTCCATA 
CTAAGCGGCG CCTCCTCCTC CTCGCCGATG AGCGGGGAGC CGGCCAAGAA GAAGGGCGTG 
CGCTTCGAGG GAATTCCGGA CACACTGCCC CGATCACGCA GCTACTCCGG TAACGGAGCG 
GGAACGAGTG GCGGCGGCGA GAGGGAGCGG GATCGGGACA AGGACAAGGA GGGAGGAGGT 
CGCCATGGTC ACGGTCACTC CTCGCGAAGG CGAAGACGTC GCAAGTCCTC CTCCAGCTCG 
TCGCACCATC GCAGCGGCAG CGGTCATCGC TCCCACTCGA CCACTCGAGC GGACACCTAT 
GCACCTGCGC AACCCCTATC CTCGTCCTAC CAGGGTCCAC CTTCGGTTCT GCAGGCTGCC 
AACCTGGTCC ACGAGTCACC CAGCCGACAG CAGAGGGAAC GGGAGCGCGA GCGGGAACGC 
GAGGAGTCCG AGGAGTCTGA CGTGTGCTCC ACCTGCTCCT CGAGCTCCTC CAGCTCGGAG 
GACTACATGA TGATGTACCA GCTGCCGCAG AGGCGCCACT ACGGCGGCGT GCGCGTGAGC 
TATGTGCCCA ACGATGCACT GGCCTACGAT CGGAAGAGGA AGCCCAGTGA ACTGGGCGGC 
GACAAGGACA AGAACTGCAT CATCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pk ( NM_165512.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724538.1 Drosophila melanogaster prickle (pk), transcript variant C, mRNA.

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CloneID	OFa11398
Clone ID Related Accession (Same CDS sequence)	NM_165512.2
Accession Version	NM_165512.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	prickle, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165512.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCC TGTCAACCGG TGGAGGAGCA GGAGGCTCCA GCGGAGGACC GGGGGGAGCA 
GATGCTGCCG CTGCTCCTGC TGCTGGCCAG GCAACAGTAA CGGCCACTGG CAACATGGAG 
CCTGCGATGG TGCCACGTAC CGCCAATTTG CTGGCATGCA AACAATGGTG GCGCGTCTGC 
TTCTTGTACG GGGACCAGCA GAAATATTAT CGACAGCTCT ATAGCAAGGC GGCGGCCCAG 
CGGCTGGCGG ACGCTAATCA GGAACCCGAC AATGCCCGCG ATCGGGAGTA CGACACCGTG 
GACTGCGATC TTATCGCCGG GCAATTAGAT GCCGTCGAGG ATGCGGACGA TGGCATCGAT 
CTAGGCGATC ACTCGTCGAC CCCGAAGGGA GGTGCGACGA CGGCGGGCCG TCCGCTCTTC 
CCGCATTCCT CCTCACCGCG GCGCAGCAAG AAGCTCCTGA GATCCCTGCG AGCCCATGTG 
CGCGGGGAGA AGCTGCCGAA GAACGACACT ACGACGGCTA ACGAGTCCAG CGAGGTGACC 
CAGCGGAATG CCAGGGTGAC CGTTCTCGAC GACCCCTTTC TATTCGGCAT CGACGCCGAT 
CACTTGGGCG ATCTCGTGGT GCGGGGCAAG CGGTACAGTA CGCTAGACGC CACCGAGAAC 
ATGGCCAGGT TCTATGCGGA GCAGGAGGCC ACGGCCCAGG TCCTGGAGAT CATCGAGCAG 
GAAGAAGAAT CTCCCGAGCA GGAAGCTCCC AAGCCCGCCC TACCGCCCAA ACAGAAGCAG 
CAGCGTCCTG TGCCGCCACT GCCACCACCA CCAGCGAACC GGGTCACCCA GGACCAAGGA 
ACGCAACCTG CAGCACCACA AGTGCCCCTG CAACCCCTGA CCGCCGGCGA TCTTCAGTTT 
CTAAACTTGA GCCTGCGGCA GAGGAGTTTA CCGCGCAGCA TGAAGCCGTT TAAGGATGCC 
CACGATATCA GTTTCACTTT CAACGAACTG GACACGAGCG CCGAACCGGA AGTGGCGACA 
GGAGCCGCCC AGCAGGAGTC AAATGAGCCC ATCAGCCGAA CTCCGCTAAC GCAGATCTCC 
TATCTGCAGA AGATCCCCAC GCTTCCGCGC CACTTCTCGC CCAGTGGGCA GGGCCTGGCC 
ACGCCCCCAG CCCTCGGCAG CGGCGGCATG GGTCTGCCCA GCAGCTCCTC CGCCAGTGCC 
CTCTACGCGG CCCAGGCAGC AGCCGGAATC CTGCCCACCT CCCCGCTGCC ACTGCAGCGC 
CACCAGCAGT ACTTGCCGCC ACATCACCAA CAGCACCCGG GAGCTGGAAT GGGACCAGGA 
CCAGGATCGG GGGCAGCAGC AGGACCGCCC CTTGGCCCCC AGTACTCGCC AGGCTGCTCC 
GCCAATCCCA AGTACTCGAA TGCCCAGCTC CCGCCACCGC CGCACCACCA CCACCAGCTA 
TCGCCGGCTT TGTCGACGCC CTCGCCACCC TCGCTGCTCC ACCATCCCGC CGGAGGCACT 
TCCTCTGCCT CGGCCCACGC CCCCTTCCTG GGCGGACCGC ACATGGACAT GCAGCGGCAG 
TCGCACTCGG ACGACGACAG TGGCTGTGCT CTGGAGGAGT ACACCTGGGT GCCACCCGGA 
CTGCGGCCTG ATCAGGTTCG CTTGTACTTC TCGCAGATAC CCGACGACAA AGTGCCATAC 
GTCAACAGTC CGGGGGAGCA GTATCGTGTG CGACAATTGC TCCATCAACT TCCGCCGCAT 
GATAACGAGG TTCGTTACTG TCACTCACTG ACGGATGAGG AGCGCAAGGA GCTGCGGTTA 
TTCTCCACGC AGCGCAAACG CGATGCCCTG GGCCGCGGCA ACGTGCGGCA GCTGATGAGC 
GCCCGACCCT GCGACGGCTG CGACGACCTA ATCTCCACGG GCGACATCGC CGTGTTCGCC 
ACGCGACTGG GCCCCAATGC CAGCTGGCAT CCGGCGTGCT TCGCCTGCTC CGTCTGCCGC 
GAGCTCCTCG TGGACCTTAT CTACTTCCAC CGGGATGGGC GCATGTACTG CGGACGCCAC 
CACGCCGAGA CCCTAAAGCC CCGCTGCTCG GCCTGCGATG AGATTATCCT GGCAGACGAG 
TGCACGGAGG CGGAGGGCAG GGCGTGGCAC ATGAACCACT TTGCCTGCCA CGAGTGCGAC 
AAGCAGCTGG GCGGCCAGCG GTATATCATG CGGGAGGGCA AACCCTACTG TCTGCACTGC 
TTCGACGCGA TGTTTGCCGA GTACTGCGAC TACTGCGGGG AGGCCATCGG GGTGGACCAG 
GGCCAGATGA GCCACGACGG GCAACACTGG CACGCCACCG ACGAGTGCTT CTCCTGCAAC 
ACTTGCCGCT GCTCGCTTCT GGGACGCGCC TTCCTGCCGC GACGAGGGGC CATCTACTGC 
TCCATCGCCT GCAGCAAAGG AGAGCCGCCC ACGCCGTCGG ACAGCTCCGG CACCGGCATG 
TACACCACGC CAACTCCGCC CACACAGCGG GTGCGGCCCC ATCCGCAAGC GCCTCTGCCG 
GCGCGCATTC CCAGCAGCCA CGCCTCCAGC TCGCCGCCTA TGTCGCCGCA ACAGCAGCAG 
CAGCACCAGG CTACCTTCAA CCAAGCGATG TACCAGATGC AAAGCCAGCA GATGGAGGCG 
GCGGGCGGAC TGGTGGACCA GAGCAAGAGC TACGCCGCGT CCGACTCGGA TGCGGGTGTG 
GTAAAGGACC TAGAACACGG CGGACATATG GGCGGAGGAG ATCTGACGGA CTTTTCGGGT 
GGACGGGCCT CCAGCACCTC GCAGAACCTA TCGCCTCTGA ACTCACCCGG CGACTTCCAG 
CCCCATTTTC TGCCCAAGCC CATGGAACTG CAGCGCGACG GAGTGTACAA CTTCAACGAG 
ATGTCCTCTA ATCTCGATGC AGCCTGGTCA GCGAAGCCCA CAAACAGTTA CCATCTGCAG 
AGACAGCTCC TGGAGAACCC CCACACCGCC AGCATGCCGG AACTGGCGGG TAAACTGGTG 
GCGCCTCCTG CACACATGCA GCACCTGTCC CAGCTGCACG CCGTGTCCTC GCACCAGTTC 
CAGCAGCACG AGTACGCGGA CATCCTGCAT CCGCCGCCTC CTCCGCCTGG TGAGATTCCC 
GAGCTGCCCA CTCCCAACTT GAGCGTGGCC TCCACCGCCT TGCCGCCAGA GCTGATGGGC 
TCACCCACCC ACTCGGCGGG CGACAGGTCG CTGAACACGC CCATGTCCAC ACAGTCGGCC 
TCCCACGCGC CCCCACATCC GGTCTCCATA CTAAGCGGCG CCTCCTCCTC CTCGCCGATG 
AGCGGGGAGC CGGCCAAGAA GAAGGGCGTG CGCTTCGAGG GAATTCCGGA CACACTGCCC 
CGATCACGCA GCTACTCCGG TAACGGAGCG GGAACGAGTG GCGGCGGCGA GAGGGAGCGG 
GATCGGGACA AGGACAAGGA GGGAGGAGGT CGCCATGGTC ACGGTCACTC CTCGCGAAGG 
CGAAGACGTC GCAAGTCCTC CTCCAGCTCG TCGCACCATC GCAGCGGCAG CGGTCATCGC 
TCCCACTCGA CCACTCGAGC GGACACCTAT GCACCTGCGC AACCCCTATC CTCGTCCTAC 
CAGGGTCCAC CTTCGGTTCT GCAGGCTGCC AACCTGGTCC ACGAGTCACC CAGCCGACAG 
CAGAGGGAAC GGGAGCGCGA GCGGGAACGC GAGGAGTCCG AGGAGTCTGA CGTGTGCTCC 
ACCTGCTCCT CGAGCTCCTC CAGCTCGGAG GACTACATGA TGATGTACCA GCTGCCGCAG 
AGGCGCCACT ACGGCGGCGT GCGCGTGAGC TATGTGCCCA ACGATGCACT GGCCTACGAT 
CGGAAGAGGA AGCCCAGTGA ACTGGGCGGC GACAAGGACA AGAACTGCAT CATCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724538.1
CDS	766..4665
Translation	MSSLSTGGGAGGSSGGPGGADAAAAPAAGQATVTATGNMEPAMVPRTANLLACKQWWRVCFLYGDQQKYYRQLYSKAAAQRLADANQEPDNARDREYDTVDCDLIAGQLDAVEDADDGIDLGDHSSTPKGGATTAGRPLFPHSSSPRRSKKLLRSLRAHVRGEKLPKNDTTTANESSEVTQRNARVTVLDDPFLFGIDADHLGDLVVRGKRYSTLDATENMARFYAEQEATAQVLEIIEQEEESPEQEAPKPALPPKQKQQRPVPPLPPPPANRVTQDQGTQPAAPQVPLQPLTAGDLQFLNLSLRQRSLPRSMKPFKDAHDISFTFNELDTSAEPEVATGAAQQESNEPISRTPLTQISYLQKIPTLPRHFSPSGQGLATPPALGSGGMGLPSSSSASALYAAQAAAGILPTSPLPLQRHQQYLPPHHQQHPGAGMGPGPGSGAAAGPPLGPQYSPGCSANPKYSNAQLPPPPHHHHQLSPALSTPSPPSLLHHPAGGTSSASAHAPFLGGPHMDMQRQSHSDDDSGCALEEYTWVPPGLRPDQVRLYFSQIPDDKVPYVNSPGEQYRVRQLLHQLPPHDNEVRYCHSLTDEERKELRLFSTQRKRDALGRGNVRQLMSARPCDGCDDLISTGDIAVFATRLGPNASWHPACFACSVCRELLVDLIYFHRDGRMYCGRHHAETLKPRCSACDEIILADECTEAEGRAWHMNHFACHECDKQLGGQRYIMREGKPYCLHCFDAMFAEYCDYCGEAIGVDQGQMSHDGQHWHATDECFSCNTCRCSLLGRAFLPRRGAIYCSIACSKGEPPTPSDSSGTGMYTTPTPPTQRVRPHPQAPLPARIPSSHASSSPPMSPQQQQQHQATFNQAMYQMQSQQMEAAGGLVDQSKSYAASDSDAGVVKDLEHGGHMGGGDLTDFSGGRASSTSQNLSPLNSPGDFQPHFLPKPMELQRDGVYNFNEMSSNLDAAWSAKPTNSYHLQRQLLENPHTASMPELAGKLVAPPAHMQHLSQLHAVSSHQFQQHEYADILHPPPPPPGEIPELPTPNLSVASTALPPELMGSPTHSAGDRSLNTPMSTQSASHAPPHPVSILSGASSSSPMSGEPAKKKGVRFEGIPDTLPRSRSYSGNGAGTSGGGERERDRDKDKEGGGRHGHGHSSRRRRRRKSSSSSSHHRSGSGHRSHSTTRADTYAPAQPLSSSYQGPPSVLQAANLVHESPSRQQREREREREREESEESDVCSTCSSSSSSSEDYMMMYQLPQRRHYGGVRVSYVPNDALAYDRKRKPSELGGDKDKNCIIS

Target ORF information:

RefSeq Version	NM_165512.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster prickle (pk), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165512.2

ORF Insert Sequence:

ATGAGCAGCC TGTCAACCGG TGGAGGAGCA GGAGGCTCCA GCGGAGGACC GGGGGGAGCA 
GATGCTGCCG CTGCTCCTGC TGCTGGCCAG GCAACAGTAA CGGCCACTGG CAACATGGAG 
CCTGCGATGG TGCCACGTAC CGCCAATTTG CTGGCATGCA AACAATGGTG GCGCGTCTGC 
TTCTTGTACG GGGACCAGCA GAAATATTAT CGACAGCTCT ATAGCAAGGC GGCGGCCCAG 
CGGCTGGCGG ACGCTAATCA GGAACCCGAC AATGCCCGCG ATCGGGAGTA CGACACCGTG 
GACTGCGATC TTATCGCCGG GCAATTAGAT GCCGTCGAGG ATGCGGACGA TGGCATCGAT 
CTAGGCGATC ACTCGTCGAC CCCGAAGGGA GGTGCGACGA CGGCGGGCCG TCCGCTCTTC 
CCGCATTCCT CCTCACCGCG GCGCAGCAAG AAGCTCCTGA GATCCCTGCG AGCCCATGTG 
CGCGGGGAGA AGCTGCCGAA GAACGACACT ACGACGGCTA ACGAGTCCAG CGAGGTGACC 
CAGCGGAATG CCAGGGTGAC CGTTCTCGAC GACCCCTTTC TATTCGGCAT CGACGCCGAT 
CACTTGGGCG ATCTCGTGGT GCGGGGCAAG CGGTACAGTA CGCTAGACGC CACCGAGAAC 
ATGGCCAGGT TCTATGCGGA GCAGGAGGCC ACGGCCCAGG TCCTGGAGAT CATCGAGCAG 
GAAGAAGAAT CTCCCGAGCA GGAAGCTCCC AAGCCCGCCC TACCGCCCAA ACAGAAGCAG 
CAGCGTCCTG TGCCGCCACT GCCACCACCA CCAGCGAACC GGGTCACCCA GGACCAAGGA 
ACGCAACCTG CAGCACCACA AGTGCCCCTG CAACCCCTGA CCGCCGGCGA TCTTCAGTTT 
CTAAACTTGA GCCTGCGGCA GAGGAGTTTA CCGCGCAGCA TGAAGCCGTT TAAGGATGCC 
CACGATATCA GTTTCACTTT CAACGAACTG GACACGAGCG CCGAACCGGA AGTGGCGACA 
GGAGCCGCCC AGCAGGAGTC AAATGAGCCC ATCAGCCGAA CTCCGCTAAC GCAGATCTCC 
TATCTGCAGA AGATCCCCAC GCTTCCGCGC CACTTCTCGC CCAGTGGGCA GGGCCTGGCC 
ACGCCCCCAG CCCTCGGCAG CGGCGGCATG GGTCTGCCCA GCAGCTCCTC CGCCAGTGCC 
CTCTACGCGG CCCAGGCAGC AGCCGGAATC CTGCCCACCT CCCCGCTGCC ACTGCAGCGC 
CACCAGCAGT ACTTGCCGCC ACATCACCAA CAGCACCCGG GAGCTGGAAT GGGACCAGGA 
CCAGGATCGG GGGCAGCAGC AGGACCGCCC CTTGGCCCCC AGTACTCGCC AGGCTGCTCC 
GCCAATCCCA AGTACTCGAA TGCCCAGCTC CCGCCACCGC CGCACCACCA CCACCAGCTA 
TCGCCGGCTT TGTCGACGCC CTCGCCACCC TCGCTGCTCC ACCATCCCGC CGGAGGCACT 
TCCTCTGCCT CGGCCCACGC CCCCTTCCTG GGCGGACCGC ACATGGACAT GCAGCGGCAG 
TCGCACTCGG ACGACGACAG TGGCTGTGCT CTGGAGGAGT ACACCTGGGT GCCACCCGGA 
CTGCGGCCTG ATCAGGTTCG CTTGTACTTC TCGCAGATAC CCGACGACAA AGTGCCATAC 
GTCAACAGTC CGGGGGAGCA GTATCGTGTG CGACAATTGC TCCATCAACT TCCGCCGCAT 
GATAACGAGG TTCGTTACTG TCACTCACTG ACGGATGAGG AGCGCAAGGA GCTGCGGTTA 
TTCTCCACGC AGCGCAAACG CGATGCCCTG GGCCGCGGCA ACGTGCGGCA GCTGATGAGC 
GCCCGACCCT GCGACGGCTG CGACGACCTA ATCTCCACGG GCGACATCGC CGTGTTCGCC 
ACGCGACTGG GCCCCAATGC CAGCTGGCAT CCGGCGTGCT TCGCCTGCTC CGTCTGCCGC 
GAGCTCCTCG TGGACCTTAT CTACTTCCAC CGGGATGGGC GCATGTACTG CGGACGCCAC 
CACGCCGAGA CCCTAAAGCC CCGCTGCTCG GCCTGCGATG AGATTATCCT GGCAGACGAG 
TGCACGGAGG CGGAGGGCAG GGCGTGGCAC ATGAACCACT TTGCCTGCCA CGAGTGCGAC 
AAGCAGCTGG GCGGCCAGCG GTATATCATG CGGGAGGGCA AACCCTACTG TCTGCACTGC 
TTCGACGCGA TGTTTGCCGA GTACTGCGAC TACTGCGGGG AGGCCATCGG GGTGGACCAG 
GGCCAGATGA GCCACGACGG GCAACACTGG CACGCCACCG ACGAGTGCTT CTCCTGCAAC 
ACTTGCCGCT GCTCGCTTCT GGGACGCGCC TTCCTGCCGC GACGAGGGGC CATCTACTGC 
TCCATCGCCT GCAGCAAAGG AGAGCCGCCC ACGCCGTCGG ACAGCTCCGG CACCGGCATG 
TACACCACGC CAACTCCGCC CACACAGCGG GTGCGGCCCC ATCCGCAAGC GCCTCTGCCG 
GCGCGCATTC CCAGCAGCCA CGCCTCCAGC TCGCCGCCTA TGTCGCCGCA ACAGCAGCAG 
CAGCACCAGG CTACCTTCAA CCAAGCGATG TACCAGATGC AAAGCCAGCA GATGGAGGCG 
GCGGGCGGAC TGGTGGACCA GAGCAAGAGC TACGCCGCGT CCGACTCGGA TGCGGGTGTG 
GTAAAGGACC TAGAACACGG CGGACATATG GGCGGAGGAG ATCTGACGGA CTTTTCGGGT 
GGACGGGCCT CCAGCACCTC GCAGAACCTA TCGCCTCTGA ACTCACCCGG CGACTTCCAG 
CCCCATTTTC TGCCCAAGCC CATGGAACTG CAGCGCGACG GAGTGTACAA CTTCAACGAG 
ATGTCCTCTA ATCTCGATGC AGCCTGGTCA GCGAAGCCCA CAAACAGTTA CCATCTGCAG 
AGACAGCTCC TGGAGAACCC CCACACCGCC AGCATGCCGG AACTGGCGGG TAAACTGGTG 
GCGCCTCCTG CACACATGCA GCACCTGTCC CAGCTGCACG CCGTGTCCTC GCACCAGTTC 
CAGCAGCACG AGTACGCGGA CATCCTGCAT CCGCCGCCTC CTCCGCCTGG TGAGATTCCC 
GAGCTGCCCA CTCCCAACTT GAGCGTGGCC TCCACCGCCT TGCCGCCAGA GCTGATGGGC 
TCACCCACCC ACTCGGCGGG CGACAGGTCG CTGAACACGC CCATGTCCAC ACAGTCGGCC 
TCCCACGCGC CCCCACATCC GGTCTCCATA CTAAGCGGCG CCTCCTCCTC CTCGCCGATG 
AGCGGGGAGC CGGCCAAGAA GAAGGGCGTG CGCTTCGAGG GAATTCCGGA CACACTGCCC 
CGATCACGCA GCTACTCCGG TAACGGAGCG GGAACGAGTG GCGGCGGCGA GAGGGAGCGG 
GATCGGGACA AGGACAAGGA GGGAGGAGGT CGCCATGGTC ACGGTCACTC CTCGCGAAGG 
CGAAGACGTC GCAAGTCCTC CTCCAGCTCG TCGCACCATC GCAGCGGCAG CGGTCATCGC 
TCCCACTCGA CCACTCGAGC GGACACCTAT GCACCTGCGC AACCCCTATC CTCGTCCTAC 
CAGGGTCCAC CTTCGGTTCT GCAGGCTGCC AACCTGGTCC ACGAGTCACC CAGCCGACAG 
CAGAGGGAAC GGGAGCGCGA GCGGGAACGC GAGGAGTCCG AGGAGTCTGA CGTGTGCTCC 
ACCTGCTCCT CGAGCTCCTC CAGCTCGGAG GACTACATGA TGATGTACCA GCTGCCGCAG 
AGGCGCCACT ACGGCGGCGT GCGCGTGAGC TATGTGCCCA ACGATGCACT GGCCTACGAT 
CGGAAGAGGA AGCCCAGTGA ACTGGGCGGC GACAAGGACA AGAACTGCAT CATCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pk cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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