fz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fz
Entrez Gene ID	45307
Full Name	frizzled
Synonyms	CG17697,CG3646,DFZ1,DFz,DFz1,Dfz,Dfz1,Dm Fz1,Dmel\CG17697,FZ,Frz,Fz,Fz1,dFz,frz,fz1,fz[[1]]
General protein information	Preferred Names frizzled Names CG17697 gene product from transcript CG17697-RA CG17697-PA CG17697-PC Frizzled1 frizzled 1 frizzled[[1]] fz-PA fz-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 70D4-70D5\|3-41.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_080073.3	NP_524812.1	frizzled, isoform A
NM_001274907.1	NP_001261836.1	frizzled, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FZD1	XP_519190.2
	FZD1	XP_001092281.1
Rattus norvegicus (Norway rat)	Fzd1	NP_067089.2
Homo sapiens (human)	FZD1	NP_003496.1
Mus musculus (house mouse)	Fzd1	NP_067432.2
Gallus gallus (chicken)	FZD1	NP_001025508.1
Danio rerio (zebrafish)	fzd7a	NP_571214.1
Drosophila melanogaster (fruit fly)	fz	NP_524812.1
Bos taurus (cattle)	FZD1	NP_001094518.1
Canis lupus familiaris (dog)	FZD1	XP_539411.3
Danio rerio (zebrafish)	fzd7b	NP_739569.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The transmembrane proteins Frizzled and Flamingo form a stoichiometric nucleus in the complex, while the relative levels of the other four core proteins can vary independently.we show that robust cell polarization is achieved over a range of complex stoichiometries but is dependent on maintaining appropriate levels of the components Frizzled and Strabismus.
Title: Robust Asymmetric Localization of Planar Polarity Proteins Is Associated with Organization into Signalosome-like Domains of Variable Stoichiometry.

instructive input of Frizzled-Planar cell polarity (Fz/PCP) signalling into polarized centriole positioning in Drosophila wings, is reported.
Title: Positioning of centrioles is a conserved readout of Frizzled planar cell polarity signalling.

These data are the first to show that Netrin and Frazzled regulate the placement of gap junctions presynaptically at a synapse.
Title: Netrin and Frazzled regulate presynaptic gap junctions at a Drosophila giant synapse.

Frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2.
Title: The Frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2.

We found no evidence for an essential requirement of the Fz C-terminal tail downstream of the KTxxxW motif in planar polarity and canonical Wnt signaling.
Title: Structure-function dissection of the frizzled receptor in Drosophila melanogaster suggests different mechanisms of action in planar polarity and canonical Wnt signaling.

The following fz gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fz cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19398	NM_080073.3 Latest version!	Drosophila melanogaster frizzled (fz), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa19903	NM_001274907.1 Latest version!	Drosophila melanogaster frizzled (fz), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fz ( NM_080073.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524812.1 Drosophila melanogaster frizzled (fz), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa19398
Clone ID Related Accession (Same CDS sequence)	NM_080073.3
Accession Version	NM_080073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frizzled, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080073.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGCGTC AAATCCTGTT TATTTTACCC ACCCTGATAC AGGGGGTCCA GCGCTACGAT 
CAGAGCCCCC TCGATGCGAG TCCGTATTAT CGCAGCGGCG GGGGATTAAT GGCCAGTTCT 
GGGACCGAAC TAGATGGACT GCCACATCAC AATCGCTGTG AACCCATCAC CATATCGATC 
TGCAAGAATA TACCATATAA CATGACCATT ATGCCAAATC TTATTGGCCA TACCAAGCAG 
GAGGAGGCGG GTCTGGAGGT CCATCAGTTT GCTCCGCTCG TGAAGATCGG CTGCAGTGAT 
GACCTCCAGT TGTTCCTCTG TTCCCTGTAC GTTCCGGTCT GCACAATTTT GGAGCGACCC 
ATTCCGCCTT GTCGATCTCT GTGCGAATCG GCGCGGGTAT GTGAAAAGTT AATGAAAACC 
TACAACTTTA ACTGGCCGGA AAATCTGGAG TGCTCCAAAT TTCCCGTCCA TGGAGGCGAG 
GATTTGTGCG TGGCGGAGAA TACCACATCA TCGGCCTCCA CGGCGGCCAC GCCCACAAGG 
AGTGTGGCTA AGGTCACTAC CCGTAAACAC CAGACGGGCG TAGAAAGTCC GCACCGAAAC 
ATTGGATTCG TGTGCCCCGT GCAACTGAAA ACGCCTCTGG GAATGGGCTA CGAACTAAAA 
GTTGGCGGAA AGGATCTGCA TGACTGTGGA GCCCCATGTC ATGCCATGTT CTTCCCGGAG 
AGAGAAAGGA CTGTGCTTCG ATACTGGGTT GGATCCTGGG CAGCGGTCTG TGTAGCCAGC 
TGCTTGTTTA CGGTGCTCAC CTTCTTGATT GACTCGTCGC GTTTTCGCTA CCCGGAGAGG 
GCCATTGTCT TCTTGGCCGT TTGCTACTTG GTAGTTGGAT GTGCCTACGT GGCGGGACTG 
GGAGCGGGCG ACTCTGTGTC GTGCCGCGAA CCATTTCCGC CGCCCGTCAA ACTCGGCCGC 
CTGCAGATGA TGTCCACCAT CACCCAGGGC CACCGACAAA CCACGTCCTG CACGGTTTTA 
TTCATGGCAC TCTACTTCTG CTGCATGGCG GCCTTCGCGT GGTGGTCGTG TCTGGCATTC 
GCCTGGTTTT TGGCCGCTGG CCTCAAATGG GGCCACGAGG CGATTGAGAA CAAGTCGCAC 
TTATTCCACC TGGTTGCCTG GGCGGTGCCC GCCCTTCAGA CCATCTCCGT TCTGGCCCTG 
GCTAAAGTTG AAGGTGACAT CCTTTCTGGC GTTTGTTTCG TGGGCCAGCT GGACACGCAC 
TCCCTGGGCG CGTTCCTGAT CCTTCCACTC TGCATTTATC TCTCGATCGG AGCACTATTC 
CTGCTGGCCG GATTTATTTC GCTTTTCCGG ATCCGGACAG TTATGAAAAC GGACGGAAAG 
AGGACAGACA AACTGGAGCG CCTGATGTTG CGAATAGGTT TCTTCTCTGG ACTTTTCATT 
CTGCCCGCCG TGGGATTACT GGGCTGCTTG TTCTACGAGT ACTACAACTT TGACGAGTGG 
ATGATCCAAT GGCACAGGGA TATCTGCAAG CCCTTCTCAA TTCCGTGCCC GGCAGCCAGG 
GCGCCGGGAT CTCCAGAAGC CCGCCCCATC TTTCAGATCT TTATGGTCAA GTACCTTTGC 
TCCATGCTGG TGGGGGTCAC TTCCAGCGTT TGGCTGTATT CCAGCAAGAC GATGGTCAGC 
TGGCGGAACT TCGTGGAGAG GTTGCAGGGC AAGGAGCCCC GGACCCGGGC GCAGGCGTAC 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524812.1
CDS	713..2458
Translation	MWRQILFILPTLIQGVQRYDQSPLDASPYYRSGGGLMASSGTELDGLPHHNRCEPITISICKNIPYNMTIMPNLIGHTKQEEAGLEVHQFAPLVKIGCSDDLQLFLCSLYVPVCTILERPIPPCRSLCESARVCEKLMKTYNFNWPENLECSKFPVHGGEDLCVAENTTSSASTAATPTRSVAKVTTRKHQTGVESPHRNIGFVCPVQLKTPLGMGYELKVGGKDLHDCGAPCHAMFFPERERTVLRYWVGSWAAVCVASCLFTVLTFLIDSSRFRYPERAIVFLAVCYLVVGCAYVAGLGAGDSVSCREPFPPPVKLGRLQMMSTITQGHRQTTSCTVLFMALYFCCMAAFAWWSCLAFAWFLAAGLKWGHEAIENKSHLFHLVAWAVPALQTISVLALAKVEGDILSGVCFVGQLDTHSLGAFLILPLCIYLSIGALFLLAGFISLFRIRTVMKTDGKRTDKLERLMLRIGFFSGLFILPAVGLLGCLFYEYYNFDEWMIQWHRDICKPFSIPCPAARAPGSPEARPIFQIFMVKYLCSMLVGVTSSVWLYSSKTMVSWRNFVERLQGKEPRTRAQAYV

Target ORF information:

RefSeq Version	NM_080073.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frizzled (fz), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080073.3

ORF Insert Sequence:

ATGTGGCGTC AAATCCTGTT TATTTTACCC ACCCTGATAC AGGGGGTCCA GCGCTACGAT 
CAGAGCCCCC TCGATGCGAG TCCGTATTAT CGCAGCGGCG GGGGATTAAT GGCCAGTTCT 
GGGACCGAAC TAGATGGACT GCCACATCAC AATCGCTGTG AACCCATCAC CATATCGATC 
TGCAAGAATA TACCATATAA CATGACCATT ATGCCAAATC TTATTGGCCA TACCAAGCAG 
GAGGAGGCGG GTCTGGAGGT CCATCAGTTT GCTCCGCTCG TGAAGATCGG CTGCAGTGAT 
GACCTCCAGT TGTTCCTCTG TTCCCTGTAC GTTCCGGTCT GCACAATTTT GGAGCGACCC 
ATTCCGCCTT GTCGATCTCT GTGCGAATCG GCGCGGGTAT GTGAAAAGTT AATGAAAACC 
TACAACTTTA ACTGGCCGGA AAATCTGGAG TGCTCCAAAT TTCCCGTCCA TGGAGGCGAG 
GATTTGTGCG TGGCGGAGAA TACCACATCA TCGGCCTCCA CGGCGGCCAC GCCCACAAGG 
AGTGTGGCTA AGGTCACTAC CCGTAAACAC CAGACGGGCG TAGAAAGTCC GCACCGAAAC 
ATTGGATTCG TGTGCCCCGT GCAACTGAAA ACGCCTCTGG GAATGGGCTA CGAACTAAAA 
GTTGGCGGAA AGGATCTGCA TGACTGTGGA GCCCCATGTC ATGCCATGTT CTTCCCGGAG 
AGAGAAAGGA CTGTGCTTCG ATACTGGGTT GGATCCTGGG CAGCGGTCTG TGTAGCCAGC 
TGCTTGTTTA CGGTGCTCAC CTTCTTGATT GACTCGTCGC GTTTTCGCTA CCCGGAGAGG 
GCCATTGTCT TCTTGGCCGT TTGCTACTTG GTAGTTGGAT GTGCCTACGT GGCGGGACTG 
GGAGCGGGCG ACTCTGTGTC GTGCCGCGAA CCATTTCCGC CGCCCGTCAA ACTCGGCCGC 
CTGCAGATGA TGTCCACCAT CACCCAGGGC CACCGACAAA CCACGTCCTG CACGGTTTTA 
TTCATGGCAC TCTACTTCTG CTGCATGGCG GCCTTCGCGT GGTGGTCGTG TCTGGCATTC 
GCCTGGTTTT TGGCCGCTGG CCTCAAATGG GGCCACGAGG CGATTGAGAA CAAGTCGCAC 
TTATTCCACC TGGTTGCCTG GGCGGTGCCC GCCCTTCAGA CCATCTCCGT TCTGGCCCTG 
GCTAAAGTTG AAGGTGACAT CCTTTCTGGC GTTTGTTTCG TGGGCCAGCT GGACACGCAC 
TCCCTGGGCG CGTTCCTGAT CCTTCCACTC TGCATTTATC TCTCGATCGG AGCACTATTC 
CTGCTGGCCG GATTTATTTC GCTTTTCCGG ATCCGGACAG TTATGAAAAC GGACGGAAAG 
AGGACAGACA AACTGGAGCG CCTGATGTTG CGAATAGGTT TCTTCTCTGG ACTTTTCATT 
CTGCCCGCCG TGGGATTACT GGGCTGCTTG TTCTACGAGT ACTACAACTT TGACGAGTGG 
ATGATCCAAT GGCACAGGGA TATCTGCAAG CCCTTCTCAA TTCCGTGCCC GGCAGCCAGG 
GCGCCGGGAT CTCCAGAAGC CCGCCCCATC TTTCAGATCT TTATGGTCAA GTACCTTTGC 
TCCATGCTGG TGGGGGTCAC TTCCAGCGTT TGGCTGTATT CCAGCAAGAC GATGGTCAGC 
TGGCGGAACT TCGTGGAGAG GTTGCAGGGC AAGGAGCCCC GGACCCGGGC GCAGGCGTAC 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fz ( NM_001274907.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261836.1 Drosophila melanogaster frizzled (fz), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa19903
Clone ID Related Accession (Same CDS sequence)	NM_001274907.1
Accession Version	NM_001274907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frizzled, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGCGTC AAATCCTGTT TATTTTACCC ACCCTGATAC AGGGGGTCCA GCGCTACGAT 
CAGAGCCCCC TCGATGCGAG TCCGTATTAT CGCAGCGGCG GGGGATTAAT GGCCAGTTCT 
GGGACCGAAC TAGATGGACT GCCACATCAC AATCGCTGTG AACCCATCAC CATATCGATC 
TGCAAGAATA TACCATATAA CATGACCATT ATGCCAAATC TTATTGGCCA TACCAAGCAG 
GAGGAGGCGG GTCTGGAGGT CCATCAGTTT GCTCCGCTCG TGAAGATCGG CTGCAGTGAT 
GACCTCCAGT TGTTCCTCTG TTCCCTGTAC GTTCCGGTCT GCACAATTTT GGAGCGACCC 
ATTCCGCCTT GTCGATCTCT GTGCGAATCG GCGCGGGTAT GTGAAAAGTT AATGAAAACC 
TACAACTTTA ACTGGCCGGA AAATCTGGAG TGCTCCAAAT TTCCCGTCCA TGGAGGCGAG 
GATTTGTGCG TGGCGGAGAA TACCACATCA TCGGCCTCCA CGGCGGCCAC GCCCACAAGG 
AGTGTGGCTA AGGTCACTAC CCGTAAACAC CAGACGGGCG TAGAAAGTCC GCACCGAAAC 
ATTGGATTCG TGTGCCCCGT GCAACTGAAA ACGCCTCTGG GAATGGGCTA CGAACTAAAA 
GTTGGCGGAA AGGATCTGCA TGACTGTGGA GCCCCATGTC ATGCCATGTT CTTCCCGGAG 
AGAGAAAGGA CTGTGCTTCG ATACTGGGTT GGATCCTGGG CAGCGGTCTG TGTAGCCAGC 
TGCTTGTTTA CGGTGCTCAC CTTCTTGATT GACTCGTCGC GTTTTCGCTA CCCGGAGAGG 
GCCATTGTCT TCTTGGCCGT TTGCTACTTG GTAGTTGGAT GTGCCTACGT GGCGGGACTG 
GGAGCGGGCG ACTCTGTGTC GTGCCGCGAA CCATTTCCGC CGCCCGTCAA ACTCGGCCGC 
CTGCAGATGA TGTCCACCAT CACCCAGGGC CACCGACAAA CCACGTCCTG CACGGTTTTA 
TTCATGGCAC TCTACTTCTG CTGCATGGCG GCCTTCGCGT GGTGGTCGTG TCTGGCATTC 
GCCTGGTTTT TGGCCGCTGG CCTCAAATGG GGCCACGAGG CGATTGAGAA CAAGTCGCAC 
TTATTCCACC TGGTTGCCTG GGCGGTGCCC GCCCTTCAGA CCATCTCCGT TCTGGCCCTG 
GCTAAAGTTG AAGGTGACAT CCTTTCTGGC GTTTGTTTCG TGGGCCAGCT GGACACGCAC 
TCCCTGGGCG CGTTCCTGAT CCTTCCACTC TGCATTTATC TCTCGATCGG AGCACTATTC 
CTGCTGGCCG GATTTATTTC GCTTTTCCGG ATCCGGACAG TTATGAAAAC GGACGGAAAG 
AGGACAGACA AACTGGAGCG CCTGATGTTG CGAATAGGTT TCTTCTCTGG ACTTTTCATT 
CTGCCCGCCG TGGGATTACT GGGCTGCTTG TTCTACGAGT ACTACAACTT TGACGAGTGG 
ATGATCCAAT GGCACAGGGA TATCTGCAAG CCCTTCTCAA TTCCGTGCCC GGCAGCCAGG 
GCGCCGGGAT CTCCAGAAGC CCGCCCCATC TTTCAGATCT TTATGGTCAA GTACCTTTGC 
TCCATGCTGG TGGGGGTCAC TTCCAGCGTT TGGCTGTATT CCAGCAAGAC GATGGTCAGC 
TGGCGGAACT TCGTGGAGAG GTTGCAGGGC AAGGAGCCCC GGACCCGGGC GCAGGCGTAC 
GTCTAGTATG AGACGGGTCC GGCGGGCGGG GCCAAGTCCA CGCCCCTTAC TCCCGATCCG 
GAGGCGGCTA ACGAAAGTTA CTTAGAGTTT AGCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261836.1
CDS	713..2551
Translation	MWRQILFILPTLIQGVQRYDQSPLDASPYYRSGGGLMASSGTELDGLPHHNRCEPITISICKNIPYNMTIMPNLIGHTKQEEAGLEVHQFAPLVKIGCSDDLQLFLCSLYVPVCTILERPIPPCRSLCESARVCEKLMKTYNFNWPENLECSKFPVHGGEDLCVAENTTSSASTAATPTRSVAKVTTRKHQTGVESPHRNIGFVCPVQLKTPLGMGYELKVGGKDLHDCGAPCHAMFFPERERTVLRYWVGSWAAVCVASCLFTVLTFLIDSSRFRYPERAIVFLAVCYLVVGCAYVAGLGAGDSVSCREPFPPPVKLGRLQMMSTITQGHRQTTSCTVLFMALYFCCMAAFAWWSCLAFAWFLAAGLKWGHEAIENKSHLFHLVAWAVPALQTISVLALAKVEGDILSGVCFVGQLDTHSLGAFLILPLCIYLSIGALFLLAGFISLFRIRTVMKTDGKRTDKLERLMLRIGFFSGLFILPAVGLLGCLFYEYYNFDEWMIQWHRDICKPFSIPCPAARAPGSPEARPIFQIFMVKYLCSMLVGVTSSVWLYSSKTMVSWRNFVERLQGKEPRTRAQAYV*YETGPAGGAKSTPLTPDPEAANESYLEFST

Target ORF information:

RefSeq Version	NM_001274907.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frizzled (fz), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274907.1

ORF Insert Sequence:

ATGTGGCGTC AAATCCTGTT TATTTTACCC ACCCTGATAC AGGGGGTCCA GCGCTACGAT 
CAGAGCCCCC TCGATGCGAG TCCGTATTAT CGCAGCGGCG GGGGATTAAT GGCCAGTTCT 
GGGACCGAAC TAGATGGACT GCCACATCAC AATCGCTGTG AACCCATCAC CATATCGATC 
TGCAAGAATA TACCATATAA CATGACCATT ATGCCAAATC TTATTGGCCA TACCAAGCAG 
GAGGAGGCGG GTCTGGAGGT CCATCAGTTT GCTCCGCTCG TGAAGATCGG CTGCAGTGAT 
GACCTCCAGT TGTTCCTCTG TTCCCTGTAC GTTCCGGTCT GCACAATTTT GGAGCGACCC 
ATTCCGCCTT GTCGATCTCT GTGCGAATCG GCGCGGGTAT GTGAAAAGTT AATGAAAACC 
TACAACTTTA ACTGGCCGGA AAATCTGGAG TGCTCCAAAT TTCCCGTCCA TGGAGGCGAG 
GATTTGTGCG TGGCGGAGAA TACCACATCA TCGGCCTCCA CGGCGGCCAC GCCCACAAGG 
AGTGTGGCTA AGGTCACTAC CCGTAAACAC CAGACGGGCG TAGAAAGTCC GCACCGAAAC 
ATTGGATTCG TGTGCCCCGT GCAACTGAAA ACGCCTCTGG GAATGGGCTA CGAACTAAAA 
GTTGGCGGAA AGGATCTGCA TGACTGTGGA GCCCCATGTC ATGCCATGTT CTTCCCGGAG 
AGAGAAAGGA CTGTGCTTCG ATACTGGGTT GGATCCTGGG CAGCGGTCTG TGTAGCCAGC 
TGCTTGTTTA CGGTGCTCAC CTTCTTGATT GACTCGTCGC GTTTTCGCTA CCCGGAGAGG 
GCCATTGTCT TCTTGGCCGT TTGCTACTTG GTAGTTGGAT GTGCCTACGT GGCGGGACTG 
GGAGCGGGCG ACTCTGTGTC GTGCCGCGAA CCATTTCCGC CGCCCGTCAA ACTCGGCCGC 
CTGCAGATGA TGTCCACCAT CACCCAGGGC CACCGACAAA CCACGTCCTG CACGGTTTTA 
TTCATGGCAC TCTACTTCTG CTGCATGGCG GCCTTCGCGT GGTGGTCGTG TCTGGCATTC 
GCCTGGTTTT TGGCCGCTGG CCTCAAATGG GGCCACGAGG CGATTGAGAA CAAGTCGCAC 
TTATTCCACC TGGTTGCCTG GGCGGTGCCC GCCCTTCAGA CCATCTCCGT TCTGGCCCTG 
GCTAAAGTTG AAGGTGACAT CCTTTCTGGC GTTTGTTTCG TGGGCCAGCT GGACACGCAC 
TCCCTGGGCG CGTTCCTGAT CCTTCCACTC TGCATTTATC TCTCGATCGG AGCACTATTC 
CTGCTGGCCG GATTTATTTC GCTTTTCCGG ATCCGGACAG TTATGAAAAC GGACGGAAAG 
AGGACAGACA AACTGGAGCG CCTGATGTTG CGAATAGGTT TCTTCTCTGG ACTTTTCATT 
CTGCCCGCCG TGGGATTACT GGGCTGCTTG TTCTACGAGT ACTACAACTT TGACGAGTGG 
ATGATCCAAT GGCACAGGGA TATCTGCAAG CCCTTCTCAA TTCCGTGCCC GGCAGCCAGG 
GCGCCGGGAT CTCCAGAAGC CCGCCCCATC TTTCAGATCT TTATGGTCAA GTACCTTTGC 
TCCATGCTGG TGGGGGTCAC TTCCAGCGTT TGGCTGTATT CCAGCAAGAC GATGGTCAGC 
TGGCGGAACT TCGTGGAGAG GTTGCAGGGC AAGGAGCCCC GGACCCGGGC GCAGGCGTAC 
GTCTAGTATG AGACGGGTCC GGCGGGCGGG GCCAAGTCCA CGCCCCTTAC TCCCGATCCG 
GAGGCGGCTA ACGAAAGTTA CTTAGAGTTT AGCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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