VhaPPA1-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	VhaPPA1-1
Entrez Gene ID	45247
Full Name	Vacuolar H[+] ATPase PPA1 subunit 1
Synonyms	CG7007,Dmel\CG7007,V0c'',Vha26,VhaPPA1,vhaPPA1-1
General protein information	Preferred Names Vacuolar H[+] ATPase PPA1 subunit 1 Names CG7007 gene product from transcript CG7007-RB CG7007-PA CG7007-PB V-ATPase proton pump VhaPPA1-1-PA VhaPPA1-1-PB vacuolar H[+] ATPase PPA1 subunit 1 vacuolar H[+] ATPase subunit PPA1-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88D6-88D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260182.2	NP_001247111.1	vacuolar H[+] ATPase PPA1 subunit 1, isoform B
NM_143753.2	NP_652010.1	vacuolar H[+] ATPase PPA1 subunit 1, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP6V0B	NP_004038.1
Pan troglodytes (chimpanzee)	ATP6V0B	XP_003308092.1
	ATP6V0B	XP_001097275.1
Bos taurus (cattle)	ATP6V0B	NP_001033127.1
Rattus norvegicus (Norway rat)	Atp6v0b	NP_001100151.1
Drosophila melanogaster (fruit fly)	VhaPPA1-1	NP_001247111.1
Magnaporthe oryzae (rice blast fungus)	MGG_06108	XP_003711974.1
Arabidopsis thaliana (thale cress)	AT4G32530	NP_001119099.1
Gallus gallus (chicken)	ATP6V0B	NP_001264501.1
Caenorhabditis elegans (roundworm)	vha-4	NP_495659.1
Canis lupus familiaris (dog)	ATP6V0B	XP_539645.2
Mus musculus (house mouse)	Atp6v0b	NP_291095.1
Saccharomyces cerevisiae (baker's yeast)	VMA16	NP_011891.1
Schizosaccharomyces pombe (fission yeast)	vma16	NP_594516.1
Xenopus tropicalis (tropical clawed frog)	atp6v0b	NP_001017064.1
Danio rerio (zebrafish)	atp6v0b	NP_955855.1
Neurospora crassa	NCU09747	XP_964449.1
Oryza sativa (rice)	Os01g0610100	NP_001043542.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009334	XP_310004.2
Kluyveromyces lactis	KLLA0E11529g	XP_454470.1
Eremothecium gossypii	AGOS_ACR071W	NP_983473.1
Arabidopsis thaliana (thale cress)	AT2G25610	NP_180132.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VhaPPA1-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VhaPPA1-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06877	NM_001260182.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase PPA1 subunit 1, transcript variant B (VhaPPA1-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06877	NM_143753.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase PPA1 subunit 1, transcript variant A (VhaPPA1-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VhaPPA1-1 ( NM_001260182.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247111.1 Drosophila melanogaster vacuolar H[+] ATPase PPA1 subunit 1, transcript variant B (VhaPPA1-1), mRNA.

Price & Availability↑

CloneID	OFa06877
Clone ID Related Accession (Same CDS sequence)	NM_001260182.2 , NM_143753.2
Accession Version	NM_001260182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase PPA1 subunit 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260182.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCC AAATACGCAC CGTGGTGTCC CAAACGTTCC TGTGGCTCTT CCTCGCCGTG 
GCCACCATCC TGACCCTGTA CTTCGTGATG ACGGGCAAGG GCGAGCGGGT GAGCGTGGGC 
TGGTTCCTGG CCTCCTCAAA CCCGTACATG TGGGCCTGCC TGGGCATCGG ACTCTCCGTC 
TCGCTGTCCG TCGTGGGCGC CGCCCTGGGC ATCCATACGA CGGGCACGAG CATCGTGGGC 
GGTGGTGTGA AGGCGCCCCG CATCAAGACC AAGAATCTGA TCTCGGTCAT CTTCTGCGAG 
GCCGTGGCCA TCTACGGCCT GATCACCGCC ATCGTTCTGT CCGGCCAGCT GGAGCAGTTC 
TCGATGGAGA CGGCCCTTTC GCAGGCGGCT ATTCAGAACA CGAACTGGTT CTCCGGCTAC 
CTCATCTTCG GTGCTGGCCT GGCTGTCGGC CTGGTCAATC TGTTCTGCGG CATTGCTGTG 
GGCATTGTGG GTTCGGGTGC CGCCCTCTCG GACGCCGCCA ATGCCGCCCT GTTCGTCAAG 
ATCCTTATTG TGGAGATCTT CGGTTCGGCC ATCGGTCTGT TCGGCCTCAT CGTGGGCATC 
TACATGACCT CCAAGTCCAA GATGGGCGAC AAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247111.1
CDS	127..765
Translation	MAAQIRTVVSQTFLWLFLAVATILTLYFVMTGKGERVSVGWFLASSNPYMWACLGIGLSVSLSVVGAALGIHTTGTSIVGGGVKAPRIKTKNLISVIFCEAVAIYGLITAIVLSGQLEQFSMETALSQAAIQNTNWFSGYLIFGAGLAVGLVNLFCGIAVGIVGSGAALSDAANAALFVKILIVEIFGSAIGLFGLIVGIYMTSKSKMGDKE

Target ORF information:

RefSeq Version	NM_001260182.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase PPA1 subunit 1, transcript variant B (VhaPPA1-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260182.2

ORF Insert Sequence:

ATGGCGGCCC AAATACGCAC CGTGGTGTCC CAAACGTTCC TGTGGCTCTT CCTCGCCGTG 
GCCACCATCC TGACCCTGTA CTTCGTGATG ACGGGCAAGG GCGAGCGGGT GAGCGTGGGC 
TGGTTCCTGG CCTCCTCAAA CCCGTACATG TGGGCCTGCC TGGGCATCGG ACTCTCCGTC 
TCGCTGTCCG TCGTGGGCGC CGCCCTGGGC ATCCATACGA CGGGCACGAG CATCGTGGGC 
GGTGGTGTGA AGGCGCCCCG CATCAAGACC AAGAATCTGA TCTCGGTCAT CTTCTGCGAG 
GCCGTGGCCA TCTACGGCCT GATCACCGCC ATCGTTCTGT CCGGCCAGCT GGAGCAGTTC 
TCGATGGAGA CGGCCCTTTC GCAGGCGGCT ATTCAGAACA CGAACTGGTT CTCCGGCTAC 
CTCATCTTCG GTGCTGGCCT GGCTGTCGGC CTGGTCAATC TGTTCTGCGG CATTGCTGTG 
GGCATTGTGG GTTCGGGTGC CGCCCTCTCG GACGCCGCCA ATGCCGCCCT GTTCGTCAAG 
ATCCTTATTG TGGAGATCTT CGGTTCGGCC ATCGGTCTGT TCGGCCTCAT CGTGGGCATC 
TACATGACCT CCAAGTCCAA GATGGGCGAC AAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VhaPPA1-1 ( NM_143753.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652010.1 Drosophila melanogaster vacuolar H[+] ATPase PPA1 subunit 1, transcript variant A (VhaPPA1-1), mRNA.

Price & Availability↑

CloneID	OFa06877
Clone ID Related Accession (Same CDS sequence)	NM_001260182.2 , NM_143753.2
Accession Version	NM_143753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase PPA1 subunit 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143753.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCC AAATACGCAC CGTGGTGTCC CAAACGTTCC TGTGGCTCTT CCTCGCCGTG 
GCCACCATCC TGACCCTGTA CTTCGTGATG ACGGGCAAGG GCGAGCGGGT GAGCGTGGGC 
TGGTTCCTGG CCTCCTCAAA CCCGTACATG TGGGCCTGCC TGGGCATCGG ACTCTCCGTC 
TCGCTGTCCG TCGTGGGCGC CGCCCTGGGC ATCCATACGA CGGGCACGAG CATCGTGGGC 
GGTGGTGTGA AGGCGCCCCG CATCAAGACC AAGAATCTGA TCTCGGTCAT CTTCTGCGAG 
GCCGTGGCCA TCTACGGCCT GATCACCGCC ATCGTTCTGT CCGGCCAGCT GGAGCAGTTC 
TCGATGGAGA CGGCCCTTTC GCAGGCGGCT ATTCAGAACA CGAACTGGTT CTCCGGCTAC 
CTCATCTTCG GTGCTGGCCT GGCTGTCGGC CTGGTCAATC TGTTCTGCGG CATTGCTGTG 
GGCATTGTGG GTTCGGGTGC CGCCCTCTCG GACGCCGCCA ATGCCGCCCT GTTCGTCAAG 
ATCCTTATTG TGGAGATCTT CGGTTCGGCC ATCGGTCTGT TCGGCCTCAT CGTGGGCATC 
TACATGACCT CCAAGTCCAA GATGGGCGAC AAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652010.1
CDS	131..769
Translation	MAAQIRTVVSQTFLWLFLAVATILTLYFVMTGKGERVSVGWFLASSNPYMWACLGIGLSVSLSVVGAALGIHTTGTSIVGGGVKAPRIKTKNLISVIFCEAVAIYGLITAIVLSGQLEQFSMETALSQAAIQNTNWFSGYLIFGAGLAVGLVNLFCGIAVGIVGSGAALSDAANAALFVKILIVEIFGSAIGLFGLIVGIYMTSKSKMGDKE

Target ORF information:

RefSeq Version	NM_143753.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase PPA1 subunit 1, transcript variant A (VhaPPA1-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143753.2

ORF Insert Sequence:

ATGGCGGCCC AAATACGCAC CGTGGTGTCC CAAACGTTCC TGTGGCTCTT CCTCGCCGTG 
GCCACCATCC TGACCCTGTA CTTCGTGATG ACGGGCAAGG GCGAGCGGGT GAGCGTGGGC 
TGGTTCCTGG CCTCCTCAAA CCCGTACATG TGGGCCTGCC TGGGCATCGG ACTCTCCGTC 
TCGCTGTCCG TCGTGGGCGC CGCCCTGGGC ATCCATACGA CGGGCACGAG CATCGTGGGC 
GGTGGTGTGA AGGCGCCCCG CATCAAGACC AAGAATCTGA TCTCGGTCAT CTTCTGCGAG 
GCCGTGGCCA TCTACGGCCT GATCACCGCC ATCGTTCTGT CCGGCCAGCT GGAGCAGTTC 
TCGATGGAGA CGGCCCTTTC GCAGGCGGCT ATTCAGAACA CGAACTGGTT CTCCGGCTAC 
CTCATCTTCG GTGCTGGCCT GGCTGTCGGC CTGGTCAATC TGTTCTGCGG CATTGCTGTG 
GGCATTGTGG GTTCGGGTGC CGCCCTCTCG GACGCCGCCA ATGCCGCCCT GTTCGTCAAG 
ATCCTTATTG TGGAGATCTT CGGTTCGGCC ATCGGTCTGT TCGGCCTCAT CGTGGGCATC 
TACATGACCT CCAAGTCCAA GATGGGCGAC AAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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VhaPPA1-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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