Zfrp8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zfrp8
Entrez Gene ID	45021
Full Name	Zinc finger protein RP-8
Synonyms	137/5,CG3260,Dmel\CG3260,PDCD2,l(2)k13705,zfrp8
General protein information	Preferred Names Zinc finger protein RP-8 Names CG3260 gene product from transcript CG3260-RA CG3260-PA CG3260-PB Zfrp8-PA Zfrp8-PB programmed cell death 2 zinc finger RP8 zinc finger protein RP-8
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60B11-60B12

mRNA and Protein(s)

mRNA	Protein	Name
NM_138046.2	NP_611890.1	zinc finger protein RP-8, isoform A
NM_001299899.1	NP_001286828.1	zinc finger protein RP-8, isoform B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pdcd2	NP_113826.1
Homo sapiens (human)	PDCD2	NP_002589.2
Pan troglodytes (chimpanzee)	PDCD2	XP_520603.2
	PDCD2	XP_001086513.1
Canis lupus familiaris (dog)	PDCD2	XP_854542.1
Gallus gallus (chicken)	PDCD2	NP_001026235.1
Arabidopsis thaliana (thale cress)	AT4G02220	NP_567225.1
Xenopus tropicalis (tropical clawed frog)	pdcd2	XP_002931535.1
Drosophila melanogaster (fruit fly)	Zfrp8	NP_611890.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005582	XP_315591.4
Oryza sativa (rice)	Os03g0300200	NP_001049855.1
Bos taurus (cattle)	PDCD2	NP_001039574.1
Mus musculus (house mouse)	Pdcd2	NP_032825.2
Danio rerio (zebrafish)	pdcd2	NP_001038603.1
Caenorhabditis elegans (roundworm)	pdcd-2	NP_497896.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

demonstrate that Zfrp8 genetically interacts with Fmr1 and tral in an antagonistic manner. Zfrp8 and FMRP both control heterochromatin packaging, also in opposite ways
Title: Zfrp8 forms a complex with fragile-X mental retardation protein and regulates its localization and function.

Our results suggest that Zfrp8/PDCD2 functions at late stages of ribosome assembly and may regulate the binding of specific mRNA-RNPs to the small ribosomal subunit ultimately controlling their cytoplasmic localization and translation.
Title: Zfrp8/PDCD2 Interacts with RpS2 Connecting Ribosome Maturation and Gene-Specific Translation.

The piRNA pathway is essential in early oogenesis, and we find that nuclear localization of Zfrp8 in germline stem cells and their offspring is regulated by some piRNA pathway genes.
Title: Zfrp8/PDCD2 is required in ovarian stem cells and interacts with the piRNA pathway machinery.

Data find that the conserved factor Zfrp8/PDCD2 is essential for the maintenance of the hematopoietic stem cells, but dispensable for their daughter cells, the pluripotent precursors.
Title: Hematopoietic stem cells in Drosophila.

Zfrp8 is involved in the regulation of cell proliferation from embryonic stages onward, through the function of the centrosome, and regulates the level and localization of cell-cycle components
Title: Zfrp8, the Drosophila ortholog of PDCD2, functions in lymph gland development and controls cell proliferation.

The following Zfrp8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zfrp8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11383	NM_138046.2 Latest version!	Drosophila melanogaster zinc finger protein RP-8, transcript variant A (Zfrp8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11383	NM_001299899.1 Latest version!	Drosophila melanogaster zinc finger protein RP-8, transcript variant B (Zfrp8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zfrp8 ( NM_138046.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611890.1 Drosophila melanogaster zinc finger protein RP-8, transcript variant A (Zfrp8), mRNA.

Price & Availability↑

CloneID	OFa11383
Clone ID Related Accession (Same CDS sequence)	NM_138046.2 , NM_001299899.1
Accession Version	NM_138046.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	zinc finger protein RP-8, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_138046.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGATTG ACTTGGGATT TGCGGAAAAG AGCGACAATG GCGCCTGGCT GAGCAACCGC 
TACTTTCCCA GCAAGCTGGG AGGCCAGCCC GCATGGCTGG AACTGGAGGC CTTGCCGCCC 
ACGTCGCAGC TGCAGTGCAG CAAGTGCAGG GCACCCAAAT CCTTCCTGGC TCAGCTATAC 
GCTCCCTTCG AAGACGAGTA CAACTTTCAT CGGTCCATCT ATGTGTTCCT GTGCCGGAAT 
TCCGACTGCC AGGAGGCCCA AAATGCAAGC AATTTCACAG TCCTCAGGTC ACAGTTGCCG 
CGAAAAAATA AGTTCTTTTC GGAGGAGGAG CCGAGCGACG TGGGTCAACC CCTGCCCGCC 
GTTCCCTGCC TAAAGAAACT ATGCGCCGCC TGCGGTTGCC ATGCTCCTCA CGCCTGCAGC 
AAATGCAAGG CAATCCACTA CTGCTCACCA GAGCATCAAA GGGCCCACTG GCCACAACAC 
AAGCCAAACT GCGGAGCACC AGAAGTAGCC ACTGAGAAGC CCTTAACACA AATCGTTTTC 
CCAGAATTTG AGATTGTAAT GGACAGCAAC CCCGTGGAGT CTGGCGAGGA GGACAAGGAC 
GATGAGGCTC GCTTGGCGGA GTTCCAGGAG CTGGAGTCCA GCGGGAAGAC GGGCGACTTG 
AGCAATGTTT CCGAGGCGGA GATGGACAAG TACTTCGGAA ACTCGGCGGC CGCCGATGAC 
AAGACCTTTC GTCAGTTCAA AAAGCAAACA GCAGCCGAAC CCGATCAGAT CGTGCGCTAC 
AAACGTGGAG GCCAGCCCCT CTGGATCACG AATACCGTCA AAACGGTGGA GGACCAACTT 
AACAAGCTGC CCAATTGCAT TGCTTGCGGA GGAGAGCGTC AGTTCGAATT TCAGATTATG 
CCGCAAGCGC TGACTCTTCT GGAGGACGAA AACCTCGACT GGGGCGTCCT GGCCGTCTAC 
ACTTGCGCCA AAAGCTGCCC CATCGACGGC TACGTGGAGG AGCTTCTCAT CAAGCAGGAC 
ATTGTGGCGG AGGATCAGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611890.1
CDS	28..1071
Translation	MEIDLGFAEKSDNGAWLSNRYFPSKLGGQPAWLELEALPPTSQLQCSKCRAPKSFLAQLYAPFEDEYNFHRSIYVFLCRNSDCQEAQNASNFTVLRSQLPRKNKFFSEEEPSDVGQPLPAVPCLKKLCAACGCHAPHACSKCKAIHYCSPEHQRAHWPQHKPNCGAPEVATEKPLTQIVFPEFEIVMDSNPVESGEEDKDDEARLAEFQELESSGKTGDLSNVSEAEMDKYFGNSAAADDKTFRQFKKQTAAEPDQIVRYKRGGQPLWITNTVKTVEDQLNKLPNCIACGGERQFEFQIMPQALTLLEDENLDWGVLAVYTCAKSCPIDGYVEELLIKQDIVAEDQS

Target ORF information:

RefSeq Version	NM_138046.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster zinc finger protein RP-8, transcript variant A (Zfrp8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138046.2

ORF Insert Sequence:

ATGGAGATTG ACTTGGGATT TGCGGAAAAG AGCGACAATG GCGCCTGGCT GAGCAACCGC 
TACTTTCCCA GCAAGCTGGG AGGCCAGCCC GCATGGCTGG AACTGGAGGC CTTGCCGCCC 
ACGTCGCAGC TGCAGTGCAG CAAGTGCAGG GCACCCAAAT CCTTCCTGGC TCAGCTATAC 
GCTCCCTTCG AAGACGAGTA CAACTTTCAT CGGTCCATCT ATGTGTTCCT GTGCCGGAAT 
TCCGACTGCC AGGAGGCCCA AAATGCAAGC AATTTCACAG TCCTCAGGTC ACAGTTGCCG 
CGAAAAAATA AGTTCTTTTC GGAGGAGGAG CCGAGCGACG TGGGTCAACC CCTGCCCGCC 
GTTCCCTGCC TAAAGAAACT ATGCGCCGCC TGCGGTTGCC ATGCTCCTCA CGCCTGCAGC 
AAATGCAAGG CAATCCACTA CTGCTCACCA GAGCATCAAA GGGCCCACTG GCCACAACAC 
AAGCCAAACT GCGGAGCACC AGAAGTAGCC ACTGAGAAGC CCTTAACACA AATCGTTTTC 
CCAGAATTTG AGATTGTAAT GGACAGCAAC CCCGTGGAGT CTGGCGAGGA GGACAAGGAC 
GATGAGGCTC GCTTGGCGGA GTTCCAGGAG CTGGAGTCCA GCGGGAAGAC GGGCGACTTG 
AGCAATGTTT CCGAGGCGGA GATGGACAAG TACTTCGGAA ACTCGGCGGC CGCCGATGAC 
AAGACCTTTC GTCAGTTCAA AAAGCAAACA GCAGCCGAAC CCGATCAGAT CGTGCGCTAC 
AAACGTGGAG GCCAGCCCCT CTGGATCACG AATACCGTCA AAACGGTGGA GGACCAACTT 
AACAAGCTGC CCAATTGCAT TGCTTGCGGA GGAGAGCGTC AGTTCGAATT TCAGATTATG 
CCGCAAGCGC TGACTCTTCT GGAGGACGAA AACCTCGACT GGGGCGTCCT GGCCGTCTAC 
ACTTGCGCCA AAAGCTGCCC CATCGACGGC TACGTGGAGG AGCTTCTCAT CAAGCAGGAC 
ATTGTGGCGG AGGATCAGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfrp8 ( NM_001299899.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286828.1 Drosophila melanogaster zinc finger protein RP-8, transcript variant B (Zfrp8), mRNA.

Price & Availability↑

CloneID	OFa11383
Clone ID Related Accession (Same CDS sequence)	NM_138046.2 , NM_001299899.1
Accession Version	NM_001299899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	zinc finger protein RP-8, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGATTG ACTTGGGATT TGCGGAAAAG AGCGACAATG GCGCCTGGCT GAGCAACCGC 
TACTTTCCCA GCAAGCTGGG AGGCCAGCCC GCATGGCTGG AACTGGAGGC CTTGCCGCCC 
ACGTCGCAGC TGCAGTGCAG CAAGTGCAGG GCACCCAAAT CCTTCCTGGC TCAGCTATAC 
GCTCCCTTCG AAGACGAGTA CAACTTTCAT CGGTCCATCT ATGTGTTCCT GTGCCGGAAT 
TCCGACTGCC AGGAGGCCCA AAATGCAAGC AATTTCACAG TCCTCAGGTC ACAGTTGCCG 
CGAAAAAATA AGTTCTTTTC GGAGGAGGAG CCGAGCGACG TGGGTCAACC CCTGCCCGCC 
GTTCCCTGCC TAAAGAAACT ATGCGCCGCC TGCGGTTGCC ATGCTCCTCA CGCCTGCAGC 
AAATGCAAGG CAATCCACTA CTGCTCACCA GAGCATCAAA GGGCCCACTG GCCACAACAC 
AAGCCAAACT GCGGAGCACC AGAAGTAGCC ACTGAGAAGC CCTTAACACA AATCGTTTTC 
CCAGAATTTG AGATTGTAAT GGACAGCAAC CCCGTGGAGT CTGGCGAGGA GGACAAGGAC 
GATGAGGCTC GCTTGGCGGA GTTCCAGGAG CTGGAGTCCA GCGGGAAGAC GGGCGACTTG 
AGCAATGTTT CCGAGGCGGA GATGGACAAG TACTTCGGAA ACTCGGCGGC CGCCGATGAC 
AAGACCTTTC GTCAGTTCAA AAAGCAAACA GCAGCCGAAC CCGATCAGAT CGTGCGCTAC 
AAACGTGGAG GCCAGCCCCT CTGGATCACG AATACCGTCA AAACGGTGGA GGACCAACTT 
AACAAGCTGC CCAATTGCAT TGCTTGCGGA GGAGAGCGTC AGTTCGAATT TCAGATTATG 
CCGCAAGCGC TGACTCTTCT GGAGGACGAA AACCTCGACT GGGGCGTCCT GGCCGTCTAC 
ACTTGCGCCA AAAGCTGCCC CATCGACGGC TACGTGGAGG AGCTTCTCAT CAAGCAGGAC 
ATTGTGGCGG AGGATCAGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286828.1
CDS	28..1071
Translation	MEIDLGFAEKSDNGAWLSNRYFPSKLGGQPAWLELEALPPTSQLQCSKCRAPKSFLAQLYAPFEDEYNFHRSIYVFLCRNSDCQEAQNASNFTVLRSQLPRKNKFFSEEEPSDVGQPLPAVPCLKKLCAACGCHAPHACSKCKAIHYCSPEHQRAHWPQHKPNCGAPEVATEKPLTQIVFPEFEIVMDSNPVESGEEDKDDEARLAEFQELESSGKTGDLSNVSEAEMDKYFGNSAAADDKTFRQFKKQTAAEPDQIVRYKRGGQPLWITNTVKTVEDQLNKLPNCIACGGERQFEFQIMPQALTLLEDENLDWGVLAVYTCAKSCPIDGYVEELLIKQDIVAEDQS

Target ORF information:

RefSeq Version	NM_001299899.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster zinc finger protein RP-8, transcript variant B (Zfrp8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299899.1

ORF Insert Sequence:

ATGGAGATTG ACTTGGGATT TGCGGAAAAG AGCGACAATG GCGCCTGGCT GAGCAACCGC 
TACTTTCCCA GCAAGCTGGG AGGCCAGCCC GCATGGCTGG AACTGGAGGC CTTGCCGCCC 
ACGTCGCAGC TGCAGTGCAG CAAGTGCAGG GCACCCAAAT CCTTCCTGGC TCAGCTATAC 
GCTCCCTTCG AAGACGAGTA CAACTTTCAT CGGTCCATCT ATGTGTTCCT GTGCCGGAAT 
TCCGACTGCC AGGAGGCCCA AAATGCAAGC AATTTCACAG TCCTCAGGTC ACAGTTGCCG 
CGAAAAAATA AGTTCTTTTC GGAGGAGGAG CCGAGCGACG TGGGTCAACC CCTGCCCGCC 
GTTCCCTGCC TAAAGAAACT ATGCGCCGCC TGCGGTTGCC ATGCTCCTCA CGCCTGCAGC 
AAATGCAAGG CAATCCACTA CTGCTCACCA GAGCATCAAA GGGCCCACTG GCCACAACAC 
AAGCCAAACT GCGGAGCACC AGAAGTAGCC ACTGAGAAGC CCTTAACACA AATCGTTTTC 
CCAGAATTTG AGATTGTAAT GGACAGCAAC CCCGTGGAGT CTGGCGAGGA GGACAAGGAC 
GATGAGGCTC GCTTGGCGGA GTTCCAGGAG CTGGAGTCCA GCGGGAAGAC GGGCGACTTG 
AGCAATGTTT CCGAGGCGGA GATGGACAAG TACTTCGGAA ACTCGGCGGC CGCCGATGAC 
AAGACCTTTC GTCAGTTCAA AAAGCAAACA GCAGCCGAAC CCGATCAGAT CGTGCGCTAC 
AAACGTGGAG GCCAGCCCCT CTGGATCACG AATACCGTCA AAACGGTGGA GGACCAACTT 
AACAAGCTGC CCAATTGCAT TGCTTGCGGA GGAGAGCGTC AGTTCGAATT TCAGATTATG 
CCGCAAGCGC TGACTCTTCT GGAGGACGAA AACCTCGACT GGGGCGTCCT GGCCGTCTAC 
ACTTGCGCCA AAAGCTGCCC CATCGACGGC TACGTGGAGG AGCTTCTCAT CAAGCAGGAC 
ATTGTGGCGG AGGATCAGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Zfrp8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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