Gr5a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gr5a
Entrez Gene ID	44873
Full Name	Gustatory receptor 5a
Synonyms	CG15779,Dmel5a,Dmel\CG15779,GR5A,GR5a,GRLU.7,Gr5,Gr5A1,GrLU7,LU.7,Tre,han,tre
General protein information	Preferred Names Gustatory receptor 5a Names CG15779 gene product from transcript CG15779-RA CG15779-PA Gr5a-PA Trehalose-sensitivity gustatory receptor 5a gustatory receptor LU.7 trehalose receptor trehalose sensitivity
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5A12-5A12\|1-13.6 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078495.3	NP_511050.1	gustatory receptor 5a

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Gr5a	NP_511050.1
Drosophila melanogaster (fruit fly)	Gr64f	NP_728924.2
Drosophila melanogaster (fruit fly)	Gr64e	NP_001027106.1
Anopheles gambiae (African malaria mosquito)	GPRGR15	XP_307760.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The detection of sugar via Gr5a gustatory receptor neurons is necessary for food-promoted male aggression.
Title: How food controls aggression in Drosophila.

necessary for normal trehalose detection
Title: Drosophila Gr5a: expression pattern, ligand profile and transduction pathway.

The simplest interpretation of our results is that Gr5a and Gr64a are each capable of functioning independently of each other within individual sugar neurons and that they are the primary receptors used in the labellum to detect sugars.
Title: Two Gr genes underlie sugar reception in Drosophila.

Gustator receptors would be candidate sugar receptors because Gr5a-expressing cells function in the responses to attractive tastants.
Title: A Drosophila gustatory receptor required for the responses to sucrose, glucose, and maltose identified by mRNA tagging.

single P-element insertions in the intergenic region between Gr5a and Tre1 exert complex pleiotropic effects on fitness traits, including selective nutrient intake, life span, and resistance to starvation and heat stress
Title: Pleiotropic fitness effects of the Tre1-Gr5a region in Drosophila melanogaster.

The following Gr5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gr5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02367	NM_078495.3 Latest version!	Drosophila melanogaster gustatory receptor 5a (Gr5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gr5a ( NM_078495.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511050.1 Drosophila melanogaster gustatory receptor 5a (Gr5a), mRNA.

Price & Availability↑

CloneID	OFa02367
Clone ID Related Accession (Same CDS sequence)	NM_078495.3
Accession Version	NM_078495.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gustatory receptor 5a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078495.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGACAGC TGAAAGGGCG AAATCGGTGC AATCGTGCTG TTCGGCATCT GAAAGTTCAG 
GGCAAGATGT GGTTGAAAAA TCTTAAAAGC GGTTTGGAAC AAATTCGAGA AAGCCAGGTG 
CGGGGAACGC GAAAGAACTT CCTACACGAT GGCTCCTTCC ATGAGGCAGT TGCTCCAGTT 
CTGGCGGTGG CCCAGTGCTT TTGCCTAATG CCCGTGTGTG GAATCAGTGC TCCAACGTAC 
AGGGGATTAA GCTTCAATCG GCGCAGCTGG CGATTCTGGT ACAGCTCCTT GTACCTCTGC 
TCCACGTCGG TGGATCTGGC CTTCAGCATT CGCCGTGTGG CCCACAGTGT GCTGGATGTG 
CGCAGTGTGG AGCCCATCGT TTTTCACGTT AGCATCCTGA TCGCCTCCTG GCAGTTCCTG 
AACCTGGCCC AACTCTGGCC TGGATTGATG CGCCACTGGG CGGCGGTGGA GCGACGGCTA 
CCGGGCTACA CCTGTTGCCT GCAACGTGCT CGTCCTGCCC GTCGCCTCAA GTTGGTGGCC 
TTCGTGCTGC TGGTAGTTTC ACTGATGGAA CACCTGCTGA GCATCATTTC GGTGGTTTAC 
TACGACTTCT GCCCACGAAG AAGCGATCCC GTGGAATCCT ATTTGCTTGG CGCCAGTGCC 
CAGTTATTCG AAGTGTTCCC CTACTCCAAC TGGCTGGCTT GGCTGGGCAA GATCCAGAAT 
GTGCTGCTCA CCTTCGGCTG GAGTTACATG GACATATTCC TAATGATGCT GGGCATGGGT 
CTCAGCGAGA TGTTGGCCAG GCTGAACCGC AGCCTGGAGC AGCAGGTGCG ACAGCCCATG 
CCGGAAGCCT ATTGGACGTG GTCACGCACT CTTTATCGCT CCATAGTAGA GCTCATACGG 
GAGGTGGATG ACGCCGTGTC CGGTATAATG CTGATATCGT TTGGCAGCAA TCTGTACTTC 
ATCTGCCTGC AGCTCCTCAA GAGCATCAAC ACCATGCCCT CCTCCGCCCA CGCCGTCTAC 
TTTTACTTCT CGCTGCTGTT CCTGCTCAGC CGATCAACGG CCGTGCTCCT CTTCGTTTCG 
GCGATCAACG ACCAGGCCAG GGAGCCACTG CGTCTGCTGC GCCTTGTCCC GCTCAAAGGA 
TATCATCCGG AGGTCTTCCG CTTCGCCGCC GAATTGGCCA GCGATCAGGT GGCGCTTACG 
GGGCTCAAGT TCTTCAATGT CACCAGAAAG TTGTTCCTGG CGATGGCGGG CACGGTGGCC 
ACTTATGAAC TAGTGCTCAT TCAGTTTCAC GAGGATAAAA AGACCTGGGA CTGCTCACCC 
TTCAATCTTG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511050.1
CDS	111..1445
Translation	MRQLKGRNRCNRAVRHLKVQGKMWLKNLKSGLEQIRESQVRGTRKNFLHDGSFHEAVAPVLAVAQCFCLMPVCGISAPTYRGLSFNRRSWRFWYSSLYLCSTSVDLAFSIRRVAHSVLDVRSVEPIVFHVSILIASWQFLNLAQLWPGLMRHWAAVERRLPGYTCCLQRARPARRLKLVAFVLLVVSLMEHLLSIISVVYYDFCPRRSDPVESYLLGASAQLFEVFPYSNWLAWLGKIQNVLLTFGWSYMDIFLMMLGMGLSEMLARLNRSLEQQVRQPMPEAYWTWSRTLYRSIVELIREVDDAVSGIMLISFGSNLYFICLQLLKSINTMPSSAHAVYFYFSLLFLLSRSTAVLLFVSAINDQAREPLRLLRLVPLKGYHPEVFRFAAELASDQVALTGLKFFNVTRKLFLAMAGTVATYELVLIQFHEDKKTWDCSPFNLD

Target ORF information:

RefSeq Version	NM_078495.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gustatory receptor 5a (Gr5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078495.3

ORF Insert Sequence:

ATGCGACAGC TGAAAGGGCG AAATCGGTGC AATCGTGCTG TTCGGCATCT GAAAGTTCAG 
GGCAAGATGT GGTTGAAAAA TCTTAAAAGC GGTTTGGAAC AAATTCGAGA AAGCCAGGTG 
CGGGGAACGC GAAAGAACTT CCTACACGAT GGCTCCTTCC ATGAGGCAGT TGCTCCAGTT 
CTGGCGGTGG CCCAGTGCTT TTGCCTAATG CCCGTGTGTG GAATCAGTGC TCCAACGTAC 
AGGGGATTAA GCTTCAATCG GCGCAGCTGG CGATTCTGGT ACAGCTCCTT GTACCTCTGC 
TCCACGTCGG TGGATCTGGC CTTCAGCATT CGCCGTGTGG CCCACAGTGT GCTGGATGTG 
CGCAGTGTGG AGCCCATCGT TTTTCACGTT AGCATCCTGA TCGCCTCCTG GCAGTTCCTG 
AACCTGGCCC AACTCTGGCC TGGATTGATG CGCCACTGGG CGGCGGTGGA GCGACGGCTA 
CCGGGCTACA CCTGTTGCCT GCAACGTGCT CGTCCTGCCC GTCGCCTCAA GTTGGTGGCC 
TTCGTGCTGC TGGTAGTTTC ACTGATGGAA CACCTGCTGA GCATCATTTC GGTGGTTTAC 
TACGACTTCT GCCCACGAAG AAGCGATCCC GTGGAATCCT ATTTGCTTGG CGCCAGTGCC 
CAGTTATTCG AAGTGTTCCC CTACTCCAAC TGGCTGGCTT GGCTGGGCAA GATCCAGAAT 
GTGCTGCTCA CCTTCGGCTG GAGTTACATG GACATATTCC TAATGATGCT GGGCATGGGT 
CTCAGCGAGA TGTTGGCCAG GCTGAACCGC AGCCTGGAGC AGCAGGTGCG ACAGCCCATG 
CCGGAAGCCT ATTGGACGTG GTCACGCACT CTTTATCGCT CCATAGTAGA GCTCATACGG 
GAGGTGGATG ACGCCGTGTC CGGTATAATG CTGATATCGT TTGGCAGCAA TCTGTACTTC 
ATCTGCCTGC AGCTCCTCAA GAGCATCAAC ACCATGCCCT CCTCCGCCCA CGCCGTCTAC 
TTTTACTTCT CGCTGCTGTT CCTGCTCAGC CGATCAACGG CCGTGCTCCT CTTCGTTTCG 
GCGATCAACG ACCAGGCCAG GGAGCCACTG CGTCTGCTGC GCCTTGTCCC GCTCAAAGGA 
TATCATCCGG AGGTCTTCCG CTTCGCCGCC GAATTGGCCA GCGATCAGGT GGCGCTTACG 
GGGCTCAAGT TCTTCAATGT CACCAGAAAG TTGTTCCTGG CGATGGCGGG CACGGTGGCC 
ACTTATGAAC TAGTGCTCAT TCAGTTTCAC GAGGATAAAA AGACCTGGGA CTGCTCACCC 
TTCAATCTTG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gr5a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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