m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	m
Entrez Gene ID	44835
Full Name	miniature
Synonyms	CG9369,Dmel\CG9369,M,Min,min
General protein information	Preferred Names miniature Names CG9369 gene product from transcript CG9369-RA CG9369-PA m-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10E1-10E2\|1-36.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_132519.2	NP_572747.1	miniature

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	m	NP_572747.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000786	XP_311326.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Extracellular matrix protein Miniature is essential for proper progress of apoptosis and, presumably, epithelial-to-mesenchymal transition.
Title: The downregulation of the miniature gene does not replicate miniature loss-of-function phenotypes in Drosophila melanogaster wing to the full extent.

Miniature acts upstream from the heterotrimeric Gs protein transducing the bursicon signal in wing epithelia.
Title: Role of the gene Miniature in Drosophila wing maturation.

functions as structural component of the Drosophila cuticulin layer
Title: Molecular analysis of the Drosophila miniature-dusky ( m-dy) gene complex: m-dy mRNAs encode transmembrane proteins with similarity to C. elegans cuticulin.

The following m gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the m cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02356	NM_132519.2 Latest version!	Drosophila melanogaster miniature (m), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

m ( NM_132519.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572747.1 Drosophila melanogaster miniature (m), mRNA.

Price & Availability↑

CloneID	OFa02356
Clone ID Related Accession (Same CDS sequence)	NM_132519.2
Accession Version	NM_132519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	miniature
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_132519.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGAGCC CACAAAAAGG CCCTACACGA CTGTGGGACC TTAGGTTTAG TAGCTGTATA 
TTTATCTTAC ACTTAATGTT TAGTTTAGTC ATAGCTGGTA ATGAACTGTG GCCCATGGAA 
CGGCCAGACG GAATGCCCAA TATCGTCTCA CTGGAGGTGA TGTGCGGCAA GGATCACATG 
GATGTACATC TCACCTTTTC GCATCCATTC GAGGGCATCG TCAGCTCCAA AGGACAACAC 
AGCGATCCGC GTTGCGTTTA TGTGCCGCCC TCAACGGGCA AGACGTTCTT CTCCTTCCGC 
ATCTCGTACT CCCGCTGCGG CACCAAGCCG GATCTCAACG GACAGTTCTA TGAGAATACG 
GTGGTGGTTC AGTACGACAA GGATCTGCTG GAAGTCTGGG ATGAGGCTAA GCGATTGCGA 
TGCGAGTGGT TCAATGACTA CGAGAAGACC GCCTCCAAGC CACCGATGGT CATTGCCGAT 
CTCGATGTTA TCCAACTGGA TTTCCGTGGT GACAATGTGG ACTGCTGGAT GGAGATTCAG 
CATGGCAAGG GACCCTGGGC GCCGCCTGTT AGTGGAATTG TACCACTCGG CTCAACTTTA 
ACCCTGGTGG TGGCCATCAA TGATTATCGA GGCGAATTCG ATATGCGTGT GAAATCCTGC 
GTGGCCTCCG ATGGATCTGG TCATGTGATC AACCTGTCCG ATGAATTTGG CTGCGTGCTG 
CGACCCAAGA TGATCTCACG CTTCCTGAAG GCTCGTGCGC CCGACGAGAG GGCCACCGTG 
ATCACCTATG CCTTCTTCCA TGCCTTCAAG TTCCCGGATG CCCTCAGTGT GCACATCAAG 
TGCAAGGTGG AGATTTGTCG CCACGGCTGT CTCGATCATT GCCAGAACAC GGGCGTGGGC 
GGTGGTGGCG GCGGATCCGG CGAGAGCCTG GGATTGGGTC TCGGCCTGGG ACTGACCAAT 
GCCAATGAGC GTAAGGATGT GCACATGTCG GATGCCTTGG GCAGTAGCAG CAACGATCTG 
CTCAGGGATT TGGCACTGCC GCCGGGCGGT CAACACGGCA TGGGCATGGG AATGGGTCCA 
GATCATGACA TCTTCTACGA GGACATCATC CACGATCACA AACAAACGCT GGGCGGCGGA 
GGAATGCCAG CTGGCGGAGA TTATGGTCAC GAGAAGAGTG TTAATCTGCA GCCACGACCC 
GTGCACGAGG AACAGGAGGA GGCGGATCTT TCAGATCTAT TTGGCGATGA GGATCTTGCC 
GACTTGGACA TGGAAGGCGG CGAGGGCGAG CGGTACTTGG GTCTGAAGAA GAGCGGCAAG 
TTCCCACACG GTCCACGGCA GCTGGAGGCC CAGAAACGTA TGGGTGTACC GATGGCGGGA 
CCCCGCTCCC TGGAGCCACA TGGTCATGGC GATGAGGATG TGCCACGACC CGTCTACAGA 
CCACAGGCGG AGGCGTTGAA GCAGCCACGC GAGGATCACA TCGATTTGGA CAAGGAGGAG 
GAAGAGGAGA AGGAGAAGGA AGAGAAAACG CAGCAGGAGG ATAAGCTGAC AGGTGAAGGA 
GGTGCAGCAG CCAGCAATGA TTCCTCCAAG AGCCGTCGCC GTCGTTCGTT GGTCATTTCC 
GATCGCAAGG TGCGCAGTGC CGATGTGGGC GTCAGCGGTC TGTACGATGT TATATCCGAG 
GCGGATTTGG CCTTCTCGCC GGACTCCAAA CAGGAGGCGG TCACCGTGTT CCAGGGCAAG 
ATTAGCGAGG AGGTTGTCTA CGGCATTTGT ATGCCAGTGC CCGGCTTCAG CATTCTCTTC 
ATCGTTGTCA TTTCGACAAC GATCGTGTCC GCTCTGGTGG CCGGATCCCT GCTATATCGC 
TATCAGCTGC AAAAGGAGGC GCTGGAGAAG CAGACACCCA TGCCGGTGAC CGGAACACTG 
GCCTCCTGGA TGACCCTGCG ACTGTTTCGC CTGCGACACA TGCAACAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAACAACAA GTGAGGCAGC CGAGTTCAGG TCACGAAACG 
GTCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572747.1
CDS	436..2484
Translation	MWSPQKGPTRLWDLRFSSCIFILHLMFSLVIAGNELWPMERPDGMPNIVSLEVMCGKDHMDVHLTFSHPFEGIVSSKGQHSDPRCVYVPPSTGKTFFSFRISYSRCGTKPDLNGQFYENTVVVQYDKDLLEVWDEAKRLRCEWFNDYEKTASKPPMVIADLDVIQLDFRGDNVDCWMEIQHGKGPWAPPVSGIVPLGSTLTLVVAINDYRGEFDMRVKSCVASDGSGHVINLSDEFGCVLRPKMISRFLKARAPDERATVITYAFFHAFKFPDALSVHIKCKVEICRHGCLDHCQNTGVGGGGGGSGESLGLGLGLGLTNANERKDVHMSDALGSSSNDLLRDLALPPGGQHGMGMGMGPDHDIFYEDIIHDHKQTLGGGGMPAGGDYGHEKSVNLQPRPVHEEQEEADLSDLFGDEDLADLDMEGGEGERYLGLKKSGKFPHGPRQLEAQKRMGVPMAGPRSLEPHGHGDEDVPRPVYRPQAEALKQPREDHIDLDKEEEEEKEKEEKTQQEDKLTGEGGAAASNDSSKSRRRRSLVISDRKVRSADVGVSGLYDVISEADLAFSPDSKQEAVTVFQGKISEEVVYGICMPVPGFSILFIVVISTTIVSALVAGSLLYRYQLQKEALEKQTPMPVTGTLASWMTLRLFRLRHMQQQQQQQQQQQQQQQQVRQPSSGHETVQ

Target ORF information:

RefSeq Version	NM_132519.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster miniature (m), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132519.2

ORF Insert Sequence:

ATGTGGAGCC CACAAAAAGG CCCTACACGA CTGTGGGACC TTAGGTTTAG TAGCTGTATA 
TTTATCTTAC ACTTAATGTT TAGTTTAGTC ATAGCTGGTA ATGAACTGTG GCCCATGGAA 
CGGCCAGACG GAATGCCCAA TATCGTCTCA CTGGAGGTGA TGTGCGGCAA GGATCACATG 
GATGTACATC TCACCTTTTC GCATCCATTC GAGGGCATCG TCAGCTCCAA AGGACAACAC 
AGCGATCCGC GTTGCGTTTA TGTGCCGCCC TCAACGGGCA AGACGTTCTT CTCCTTCCGC 
ATCTCGTACT CCCGCTGCGG CACCAAGCCG GATCTCAACG GACAGTTCTA TGAGAATACG 
GTGGTGGTTC AGTACGACAA GGATCTGCTG GAAGTCTGGG ATGAGGCTAA GCGATTGCGA 
TGCGAGTGGT TCAATGACTA CGAGAAGACC GCCTCCAAGC CACCGATGGT CATTGCCGAT 
CTCGATGTTA TCCAACTGGA TTTCCGTGGT GACAATGTGG ACTGCTGGAT GGAGATTCAG 
CATGGCAAGG GACCCTGGGC GCCGCCTGTT AGTGGAATTG TACCACTCGG CTCAACTTTA 
ACCCTGGTGG TGGCCATCAA TGATTATCGA GGCGAATTCG ATATGCGTGT GAAATCCTGC 
GTGGCCTCCG ATGGATCTGG TCATGTGATC AACCTGTCCG ATGAATTTGG CTGCGTGCTG 
CGACCCAAGA TGATCTCACG CTTCCTGAAG GCTCGTGCGC CCGACGAGAG GGCCACCGTG 
ATCACCTATG CCTTCTTCCA TGCCTTCAAG TTCCCGGATG CCCTCAGTGT GCACATCAAG 
TGCAAGGTGG AGATTTGTCG CCACGGCTGT CTCGATCATT GCCAGAACAC GGGCGTGGGC 
GGTGGTGGCG GCGGATCCGG CGAGAGCCTG GGATTGGGTC TCGGCCTGGG ACTGACCAAT 
GCCAATGAGC GTAAGGATGT GCACATGTCG GATGCCTTGG GCAGTAGCAG CAACGATCTG 
CTCAGGGATT TGGCACTGCC GCCGGGCGGT CAACACGGCA TGGGCATGGG AATGGGTCCA 
GATCATGACA TCTTCTACGA GGACATCATC CACGATCACA AACAAACGCT GGGCGGCGGA 
GGAATGCCAG CTGGCGGAGA TTATGGTCAC GAGAAGAGTG TTAATCTGCA GCCACGACCC 
GTGCACGAGG AACAGGAGGA GGCGGATCTT TCAGATCTAT TTGGCGATGA GGATCTTGCC 
GACTTGGACA TGGAAGGCGG CGAGGGCGAG CGGTACTTGG GTCTGAAGAA GAGCGGCAAG 
TTCCCACACG GTCCACGGCA GCTGGAGGCC CAGAAACGTA TGGGTGTACC GATGGCGGGA 
CCCCGCTCCC TGGAGCCACA TGGTCATGGC GATGAGGATG TGCCACGACC CGTCTACAGA 
CCACAGGCGG AGGCGTTGAA GCAGCCACGC GAGGATCACA TCGATTTGGA CAAGGAGGAG 
GAAGAGGAGA AGGAGAAGGA AGAGAAAACG CAGCAGGAGG ATAAGCTGAC AGGTGAAGGA 
GGTGCAGCAG CCAGCAATGA TTCCTCCAAG AGCCGTCGCC GTCGTTCGTT GGTCATTTCC 
GATCGCAAGG TGCGCAGTGC CGATGTGGGC GTCAGCGGTC TGTACGATGT TATATCCGAG 
GCGGATTTGG CCTTCTCGCC GGACTCCAAA CAGGAGGCGG TCACCGTGTT CCAGGGCAAG 
ATTAGCGAGG AGGTTGTCTA CGGCATTTGT ATGCCAGTGC CCGGCTTCAG CATTCTCTTC 
ATCGTTGTCA TTTCGACAAC GATCGTGTCC GCTCTGGTGG CCGGATCCCT GCTATATCGC 
TATCAGCTGC AAAAGGAGGC GCTGGAGAAG CAGACACCCA TGCCGGTGAC CGGAACACTG 
GCCTCCTGGA TGACCCTGCG ACTGTTTCGC CTGCGACACA TGCAACAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAACAACAA GTGAGGCAGC CGAGTTCAGG TCACGAAACG 
GTCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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