kto cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	kto
Entrez Gene ID	44830
Full Name	kohtalo
Synonyms	Brm @ Kto,CG8491,Dmel\CG8491,Kto,Kto/Med12,MED12,Med12,SRB8,Srb8/KTO/TRAP230,Trap230,dTRAP230,dTrap230,l(3)76BDc,med12,med12/kto,psg14,siren1
General protein information	Preferred Names kohtalo Names CG8491 gene product from transcript CG8491-RA CG8491-PA CG8491-PB khotalo kto-PA kto-PB mediator complex subunit 12
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76D1-76D1\|3-46 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_080047.2	NP_524786.1	kohtalo, isoform A
NM_001259906.1	NP_001246835.1	kohtalo, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	MED12L	NP_443728.3
Pan troglodytes (chimpanzee)	MED12L	XP_516821.2
Mus musculus (house mouse)	Med12l	NP_808523.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002523	XP_312414.5
Drosophila melanogaster (fruit fly)	kto	NP_524786.1
	MED12L	XP_001107022.2
Gallus gallus (chicken)	MED12L	XP_422838.4
Canis lupus familiaris (dog)	MED12L	XP_005634712.1
Bos taurus (cattle)	MED12L	NP_001193434.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kto-Skd affects the A/P-axial development of the whole wing disc. Kto can interact with Cubitus interruptus (Ci), bind to the Ci-binding region on ptc promoter, which are both regulated by Hh signals to down-regulate ptc expression.
Title: The Kto-Skd complex can regulate ptc expression by interacting with Cubitus interruptus (Ci) in the Hedgehog signaling pathway.

Pygopus promotes Wnt target gene transcription by recruiting the mediator complex through interactions with Med12 and Med13.
Title: Pygopus activates Wingless target gene transcription through the mediator complex subunits Med12 and Med13.

Kto/Skd act as essential coactivators for Ato expression during early retinal neurogenesis.
Title: Drosophila TRAP230/240 are essential coactivators for Atonal in retinal neurogenesis.

Data show that both sklud and kohtalo are required to establish normal cell affinity differences at the anterior-posterior and dorsal-ventral compartment boundaries of the wing disc.
Title: Two subunits of the Drosophila mediator complex act together to control cell affinity.

The following kto gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kto cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19391	NM_080047.2 Latest version!	Drosophila melanogaster kohtalo (kto), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OFa19904	NM_001259906.1 Latest version!	Drosophila melanogaster kohtalo (kto), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kto ( NM_080047.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524786.1 Drosophila melanogaster kohtalo (kto), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa19391
Clone ID Related Accession (Same CDS sequence)	NM_080047.2
Accession Version	NM_080047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kohtalo, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Nov 6, 2002 this sequence version replaced NM_080047.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGTCCA TGCTGCAGGA GAAGCGTCCG CTGAAGCGCA CCCGTCTGGG TCCGCCGGAC 
ATCTATCCGC AGGACGCGAA GCAGCGAGAG GACGAGCTGA CGCCCACGAA TGTGAAGCAC 
GGATTCACGA CGACGCCACC GCTGTCCGAT GAATTCGGTA CGGCCCACAA CTCGAATGTG 
AACGCCAGCA AAGTGAGCGC ATTCTTTAGC GGCGTCCTGG CCAAGAAGGA GGAGCTGATG 
ACCCTGCCGG ACACGGGGCG CAAGAAGCAG CAGATCAACT GCAAGGACAA CTTCTGGCCC 
GTTTCGCCGC GTCGCAAGTG TACGGTGGAC GCCTGGTTTA AGGACCTCGC CGGTAACAAA 
CCCCTACTTA GCCTAGCCAA ACGGGCTCCA TCGTTCAACA AGAAGGAGGA GATCTTCATT 
ACTCTGTGCG AGAACCAGGT GAACATGCAA CGCGCCACCT GGTTCATCAA GCTGAGCGCC 
GCCTATACGC TTAGCTTCAC CGAGTCTAAG AACAAAAAGC GATCCATTTA CGACCCGGCT 
GCCGAGTGGA CCGGCAACAT GATCAAGTTC ATGAAAGAGC TGCTTCCCAA GCTCCAAGAG 
TATTATCAGC AGAACCACGA CAAGTCCTCT TCGAATGGCA CCACTTCCGG AAGCCTGACC 
GCCGCCGGTA ATGGTCCTGC CAGCAATGGC TCCACTGGCA CCAGCTCTAT TAACTCCGTC 
ACCGGTAGCA GTGCTTCCAC GAACGTAATC CCGGTACCCA GCATGGCCAG TCCACTGCCA 
CCGATTCATA GCCCAGCTAA TGGCCAACAG GCAGCTCCTG GCGGAGGAGT GAATGCCGGC 
AGTGTCATGC CGACGACGGG AAGCCTTGGC GGAGTAGTCG GTGGGCCGGG GTCTTCTGTG 
GTAGGAGGCG CAGCGGGTGC AGGAGCTGCA GTTCCACCAG GCTCTACAAT TTCTGGAATT 
GGAAGCCAGT TCGAAGATAG TCGCAATGCG CTTAAGTATT GGAAATACTG CCACCAATTG 
AGCAAGTACA TGTACGAGGA ATCACTTTTG GACCGACAGG AATTTCTAAA CTGGATCCTC 
GACCTTTTGG ACAAGATGCG TACGCAGGCA AGCTTCGACG AACCGTTGAA GAAATTGGTG 
CTCAGCTTTG CCCTTCAGTA CATGCACGAC TTCGTCCAGT CGGAGCGTCT TTGCAGAAAG 
ATGGCCTACA TAGTGTCCAA AAAACTGGCC CAGTTGCTGA ACACTGTCGT AGAACAGCAG 
ACCATCAAAG AGTTGGACGA GCCGAAGTTG CAACAAGATC CCTATGAGCT GGCGCTTCAG 
GAACAAATGA GTTGTCCCCA TCATCGGGAT ATAGTGCTGT ATTTAAGCAC GATCCTACAG 
ATCATCACAA TCGAGTGCCC CACAGCTCTG GTCTGGAGTG GGATAGCAGC TCACCGAGCA 
CCCTCCTCGT TGTTGGGTAG TCCGCTGGAC CATTTGCCTT TGGCGCCCTC AGTCTTGCCG 
ATGCCCACAC GCTGTCCGCG AACCAATCAC GAGATTCGAC GTCAATTGCG GGCCGCCGAG 
TCGGACATCG TATTGCGCAC TCAGCATGCA GAGCAGCGCT GGTTTGCAGC CAAGTGGCTG 
AGTGCCGGCA AGAATCAATA CACCAGTGTG CTGGCTACAC TGGACCACTT GGACACGCAC 
TGCTTCGATC GCATGGAACA CAATAATAGT ATCGATACCC TATACGCACA AATATTTCCC 
AGCCCAACTG TCAGTCGCAG GCGGGAGGAG GATCAGGTGG AACCTCGTCC GCCTTATGAA 
CCCAAACAAG ACAAGGACAC GGTACGCATT CTGTGCGAGT GGGCTGTGTC GGGGCAGCGT 
TGGGGAGAAC ACAGAGCCAT GGTGGTGGCC ATTCTACTTG ACAAGCGCCA GATCGACGTG 
ACAAGCACGC CGGCGGATCA ACAATCCAGT GATAAGGATG ACAAGGACTC GCTAGCTTCG 
GGCGCCGGCT TAATTGATGG CCTGCCTGTG TTTCAGCACG TGTTAATGCA CTTCCTTGAC 
CACGATGCTC CCGTCCTCGA TGAGCACGTG AGCAGTCCAC AGCAGCGCAC GGAATTTACC 
AACCTTGTTC AGCTGTTCAG TGCATTGATT CGCCACGATG TCTTTTCGCA CAACGCGTAC 
ATGCACACTC TAATTTCACG AGGAGATCTG CTCCTGGAGT CGGTGCTGGT GATCAAATCA 
GGAACAACGG CCACGAAAAC ATCGCCGCCA CCTCCAGCTC CACCACCAAC GACGACGCAC 
GGTTTCGATG ATGACGGATT CGGCGGGGGC CTGGACTTCA AACACAACGA ATTTGATGAT 
TCCAATGTGG ACGACGATTT GGACAAACTG GTGCAAAATA TTAAGGAGAA AGGACAACAG 
CACGAAGCTC CAGACAGTCC CAAGATTGGT CCTCCAGGCG ATGGAGAAAC CAATCCTGGC 
GGTAGCATAT CGCGGCATTA CGTGTACACC AAGCACTTCC CCATCCCCCA GGATGATCCC 
AGCATGAGCA GCTACAGCAG TGAAAGCAAC CAGCGATACA TCCTGCTCTT CGGAGTCGGC 
AAAGAGAGGG ACGAGAAGAA GCATGCTGTC AAGAAGATGT CTAAGGAAAT TGGAAAGCTT 
TTCACAAAAA AGTTTAGCAT AGATGTGGCG GCGGCCGGTC ATGTGAAGAA GCACTCGCGC 
AACGAGTTTA ACTTCGAAGC TACCACGTCC AAGTGCCAGC AGATGGCTTA TTTCGACCAG 
CATGTGGTCA CAGCCCAATG TGCGGCCAAT GTCCTGGAGC AGCTGAACGG ATTCGCGTTA 
GGGAACAACA ACTACCTGCC GGTACAGGAG CACGTGGCGT TCCTCTTTGA TCTGATGGAG 
CTGGCTCTTA ATATCTACAG TCTTTTGGAA CTGTGCGATA GTCTGTTGAA GGAGTTGCCT 
GAGGTGGAGC ACCAGTTGCA GCTAAAGAAG TCAAACCTAG TGCGCAGTTA CACCACTTCA 
CTTGCCCTCT ACATCGTCAG CATCCTGCGG CGCTACCACA GCTGCCTTCT TCTTTCACCG 
GAGCAGACGC TGTCTGTTTT TGAGGGAGTC TGTCGTACCA TTCGGCACGT ATCGAATCCG 
AGTGAGTGCA CTTCGGCGGA GCGCTGCATT ATTGCCTACC TGAGCGACCT ACACGAGTCG 
TGTGTTTTGC TCCAGGGAAA GGAACAATCC ACGGAGTACT ACCAGCAGCT GCAGTGCATC 
AAGCGGTTCA AGGACATATT CAACACTCCA GAGCAGCTTG ACCTACCACC TCAGGGTTAC 
AACCCACTGT TGTTGCAGGA GCTTTTCATG GCCCCTCGAC GCGGTGGTAA ACTGGATCCT 
CACTGGCTGG GAACCCTGCA CGAGTCGCCG GCCAATGTAT ACAGTTTCGT GTCTAACGCA 
TTAATAGCCG TCTGCCGAGA GACGGACAAC GAGCGGCTGA ACGATGTGGC CCTCGCATGT 
GCCGAACTGA CCGCCAGCTG CAACGTCTTG TCTGAGGAGT GGATATACGC ACTGCAGAGT 
CTGTGCAGCG GCAGTAAGAG TCCGCGTTAT CCGCACCTGG GCGGACAGGT CGACATTGGA 
CAACTTAAGA CTCACAACGC CCTGGCCGTG TTCGTATGCA TTCTGGTGGC CAGACATTGC 
TTCTCACTAG CCGACTTTGT AAGTAAGTTC GCCCTGCCTA CATTGGCCCG ATCGGTAAGC 
GCTGGTGGAG CCGAACTGAG TGTAGATGCA GAGGCAGGTG CTCGTCTTAC ATGTCACTTG 
GTGCTGAAGC TGTTTAAGAC CTTGGAGATT CCTCAACCTG GCATGTACTC GGTAAGCACT 
TCCCCGAATC CACTGCACGC TGTGGGAAAC GACTTTAGCA TTCGGCTGAG CTGCGATCGT 
CATCTTCTGG TGGGTGCCCA TAAGACCATT CCCATTGCCG CAGTGCTTGC CGTGCTCAAG 
GCCATTCTCA TTGTTGTGGA CAATGCGGCG CTTAAAACAC CATTGGCTTC CGGCAGCGGC 
ACCTCCTCTG GCGGATTGGG CGGAGCTTTT GGAAGCGGCA AGCGTAGTGG CTTCAATACA 
CCGGTCCATC CGGGAAGCAC ACCCAAGAGC AACGAACAAC GACCGGCCGA TCTCAGCCAG 
ATCCTGGGCA CCAGCGATTT ACAATTGGGC AGCAGTTTGA CAAGCGAACC CGAAGCCCTG 
CAACAGCCAT CGGTCGGAGG AATGGAGCAG ATCTCGCTGT TGGAGTTCGC CCAGGCGGTG 
CTCAAGCAGA TCTGCGCCCA GGAACACGTT CTGGAAAGAT GCCTAAAGAA CGCAGAGCAA 
CTCTGCGACA TGATAATCGA CGAAATGCTG ACCGCGAAAC AGGCACAGCG GGTTCTGCAC 
ATGATCTGCT ATCCGGAGCC TGAGTTTAAC ATCATCTCCG AACTGGATCA GCGCTCAATG 
ATCGTACGCA TACTAGAGAA TCTCGGACAG TGGACACTGC GTATCTCGTG GCTGGATCTT 
CAGCTTATGT ACCGCCAATC GCTGAGCAAC AACGCGGAGC TTAATGTTTG GCTCGACACA 
GTGGCACGCG CGGCAATCGA CGTCTTCCAC ATGGAGGAAG TTGTCCTGCC GGGTGCCGTG 
AAGGCGACCC ACAAACCCAA ACCGTCCACC TGGCTAGTGG CTCCACTCAT CGCTAAACTA 
ACACCAGCGG TGCAGGGCAG GATACTTCGA GTGGCTGGTC AGGTTCTTGA GAGCATGAAC 
TATTTCTCCA AGGTGTCCAA GTCCGATTGC AATAGCTCGG GCAGTGGTGA CGAGCGCGAG 
AAGAGCAACA GTTGTCACAG TAGCAATAGC TATGGACTGG GCGGCGTGCC CGCACGCAAC 
AAGAAGATGC CGCTTGATTA TCAGCCTTTC CTGGGTCTCA TCCTAACTTG CCTGAAAGGC 
CAGGACGAGT ACAAGGAAAA CTTACTGGTT TCGCTCTATG CCCAGTTATC ACAATGTCTG 
CAATCCTTTG CTGAGCTGGA CACAATCGGT GGCATTGATG AGCCGCAGGC GCGCGAGGAG 
ATCCTCGATG CGCTGCAGTT GCGCTTTTCG CTGGTGGGCG GCATGTTCGA GGCTATCCAA 
AAGAACAGTA CCCCCACCAC AGACTGGGCT ATTCTTCTGG CCCAATTGGT TTGCCAGGGC 
GTGGTAGATC TCAGCTGCAA TCGTGAGCTG TTCACCACCG TTGTGGACAT GTTGGCCACA 
TTGGTGCACT CAACGCTGGT CTCTGATGAC GAGAGGCACT ACATGAACCT GATGAAGAAG 
CTAAAGAAGG AAATCGGAGA GAAGAACAAC GCCTCCATTC GGGTTATTCG GCAGCTTCTT 
CCACTTTACA AGCAGCCTAC TGAGGTGATT GCGTGCGAAC ACTCTGGAAT GGACACCAAG 
GGCAACAAGA TCTGCGATAT AGACAAAAAA CAACTACGCA TTTCGGACAA ACAGCGTATC 
AGCGTGTGGG ACATCCTTGA GGGTCACAAG AACCCGGCTC CTTTGTCCTG GGTGTGGTTT 
GGTGCAGTCA AGCTGGAGCG CAAGCCCCTT ACTTACGAGG AGGCTCACCG CAACCTGAAG 
TATCACACGC ACAGTCTGGT CAAGCCCAGT AGTTATTACT ATGAACCGTT GCCACTGCCA 
CCTGAAGACA TTGAGCCTGT TCCCGAGAAG ATTTGCATAA AGGATGAAAT GAAGGCGGAC 
ACGCCCTCGT CGGTGGATCA GTCGCCAAGC GCTGTGGTTG GCGGCACGGG AAGAGGTCGC 
GGCAAGGGAA CGACCACGCG GAAACGCAAG CCGAAGAACC CGAAAACGCC ACCGGTGGTC 
AATACTCAGC AACAGCAGCC TCAGCTGGCA CAGCAGCCTC AACAGCCGCA GAATGTGCAG 
CAACAGCAGT TGCAACAGCA GCAGCAACAA CAGCAGCACA TGCAGCAGCA GCACATGCAA 
CAGCAGCAGA TGCAGCCCAA CCAAATGGGC CAGATGCCAA TGAACATGCC GATGAACATG 
CAGCAGTTTG CACCCAATCC GAACAACATG ATGCAGCAGA ATGCCATGTT ACAACAGCAG 
CAGCAGCAGC AGATGCAGCA AATGGGCAAC AACCCGATGC AGCAGCAGCT GAATGTGGGC 
GGTGGGAACG GGCAGCCAAA TCCCCAGATG AACTTCATGC AGCAGGGACC AGGGGGCGGT 
GGAGCCGGAC CGCAGGGTAT GCCTGGCCAG CAGCAACAGT GGCACAATGC GCCGCAGCAG 
CAGCAGCCGC CACAACCGTA CCACAACCAA TACGCTCCGC ATCAGCAGAA CATGCAGTCA 
AATCGCATCG AGCGCCCGCC GTTGAATGCC AACTCCAAGC AGGCGCTATC ACAGATGCTA 
CGACAACGAC AACCATTCCA GCAGCAAGCG CAACAGGGTC CGGGGGGTGG TTTCAATCCG 
ATGCAGCAAC AACCACAGGC TTCGCAACAA CAGCCCGGTC CTCAGCAGCA GATGAACCCC 
AACCAAATGC GCCAGCAGCA AATGAATCCC CAGCAAAATC CTCAGTCTGT CGCTGCATTC 
AACGCCATGC AGCAGCAGCC GCAGCAGAAC GCCCAGCAGC AACAGATGAA TCCCAACCAG 
CAGCAACAGC AGCAGTTCAT GCGCGGCGGC AACATGCGAC CCGGCATGGC GCCCAACCAG 
ATGAATCAGA TGAACATGGG AGGTCAAGGC ATGTCGCAGA ATCCGATGAT GCAGCAACAA 
ATCCCACAGA ATATGGTGGG CATGGTCAAC CCGAATGCCA ACCAAATGAT GCAGAGCGGT 
GGAGCTCAGG GAGGCAACGG TGTGGGCGTT GGTGTTGGCG TCGGCGTTGG CGGTGCCGGC 
AACAATCCCA ACATGGGCAT GGGAGGCATG CCTCAGCAAG GCATGATACA ACAGCAGCCG 
CAGCAACAGC CGCAGCAGCA GGTGCAGTTC CAGAACTTCC AGAATCAGTA CCAGCAGCAG 
CAACAGCAGG GCATGCAGCA ACAGGGCGGT GGAGCAGGCG TTGGAGTGGG CGTCGGCATG 
GCGCCCAATC AGCAGCAACA GCAGCAGGCC AACATGATGG GCAACTTCAA TCCGCAGATG 
CAGCAGGGGA ATCGCAACAA TCCCGACTTT ATGGCCGCCG CAGCGGTTGC CCAGCAGCAG 
CAACAACAAC AACAGCAACA GCGCGTTGTT CCCGGAGGAA TGATGGCCGG CAACCGGAAC 
CAGTACATGA ACCAGGCGCC CAACGTAACC ATGTCCACCA TGATGGGTCC GGGGCCGGGT 
GGCGTCGTCG GCCAGGTGCC TCCGTACGCC CGTCAGCAGT CGGCGGGAGG AGGCAAACCC 
GGCGTCCTCA ACACCCAGCA GCAGTTCCAG CAGCAGCAAC AGCAACAACA GCAGCTGAGG 
CACCAAATGA TGCAGTTGCA GGGTATGGGT GGTGGGGCAG GCGGCGGCAT GGGAGCCGGG 
CCTCAGCAGG GGGGTGGCGC CGTTGGCGGT GGCGCCGGAG GCGGAATGGT GCCGCAGCAG 
CAGAGCATGA ACCAGCAACA AACGCCGAAT CTGGTGGCTC AGCTCCAGCG GCAGAACATG 
ATGGGCCAGC AGCAGTATCA ACCACCACCG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524786.1
CDS	63..7658
Translation	MLSMLQEKRPLKRTRLGPPDIYPQDAKQREDELTPTNVKHGFTTTPPLSDEFGTAHNSNVNASKVSAFFSGVLAKKEELMTLPDTGRKKQQINCKDNFWPVSPRRKCTVDAWFKDLAGNKPLLSLAKRAPSFNKKEEIFITLCENQVNMQRATWFIKLSAAYTLSFTESKNKKRSIYDPAAEWTGNMIKFMKELLPKLQEYYQQNHDKSSSNGTTSGSLTAAGNGPASNGSTGTSSINSVTGSSASTNVIPVPSMASPLPPIHSPANGQQAAPGGGVNAGSVMPTTGSLGGVVGGPGSSVVGGAAGAGAAVPPGSTISGIGSQFEDSRNALKYWKYCHQLSKYMYEESLLDRQEFLNWILDLLDKMRTQASFDEPLKKLVLSFALQYMHDFVQSERLCRKMAYIVSKKLAQLLNTVVEQQTIKELDEPKLQQDPYELALQEQMSCPHHRDIVLYLSTILQIITIECPTALVWSGIAAHRAPSSLLGSPLDHLPLAPSVLPMPTRCPRTNHEIRRQLRAAESDIVLRTQHAEQRWFAAKWLSAGKNQYTSVLATLDHLDTHCFDRMEHNNSIDTLYAQIFPSPTVSRRREEDQVEPRPPYEPKQDKDTVRILCEWAVSGQRWGEHRAMVVAILLDKRQIDVTSTPADQQSSDKDDKDSLASGAGLIDGLPVFQHVLMHFLDHDAPVLDEHVSSPQQRTEFTNLVQLFSALIRHDVFSHNAYMHTLISRGDLLLESVLVIKSGTTATKTSPPPPAPPPTTTHGFDDDGFGGGLDFKHNEFDDSNVDDDLDKLVQNIKEKGQQHEAPDSPKIGPPGDGETNPGGSISRHYVYTKHFPIPQDDPSMSSYSSESNQRYILLFGVGKERDEKKHAVKKMSKEIGKLFTKKFSIDVAAAGHVKKHSRNEFNFEATTSKCQQMAYFDQHVVTAQCAANVLEQLNGFALGNNNYLPVQEHVAFLFDLMELALNIYSLLELCDSLLKELPEVEHQLQLKKSNLVRSYTTSLALYIVSILRRYHSCLLLSPEQTLSVFEGVCRTIRHVSNPSECTSAERCIIAYLSDLHESCVLLQGKEQSTEYYQQLQCIKRFKDIFNTPEQLDLPPQGYNPLLLQELFMAPRRGGKLDPHWLGTLHESPANVYSFVSNALIAVCRETDNERLNDVALACAELTASCNVLSEEWIYALQSLCSGSKSPRYPHLGGQVDIGQLKTHNALAVFVCILVARHCFSLADFVSKFALPTLARSVSAGGAELSVDAEAGARLTCHLVLKLFKTLEIPQPGMYSVSTSPNPLHAVGNDFSIRLSCDRHLLVGAHKTIPIAAVLAVLKAILIVVDNAALKTPLASGSGTSSGGLGGAFGSGKRSGFNTPVHPGSTPKSNEQRPADLSQILGTSDLQLGSSLTSEPEALQQPSVGGMEQISLLEFAQAVLKQICAQEHVLERCLKNAEQLCDMIIDEMLTAKQAQRVLHMICYPEPEFNIISELDQRSMIVRILENLGQWTLRISWLDLQLMYRQSLSNNAELNVWLDTVARAAIDVFHMEEVVLPGAVKATHKPKPSTWLVAPLIAKLTPAVQGRILRVAGQVLESMNYFSKVSKSDCNSSGSGDEREKSNSCHSSNSYGLGGVPARNKKMPLDYQPFLGLILTCLKGQDEYKENLLVSLYAQLSQCLQSFAELDTIGGIDEPQAREEILDALQLRFSLVGGMFEAIQKNSTPTTDWAILLAQLVCQGVVDLSCNRELFTTVVDMLATLVHSTLVSDDERHYMNLMKKLKKEIGEKNNASIRVIRQLLPLYKQPTEVIACEHSGMDTKGNKICDIDKKQLRISDKQRISVWDILEGHKNPAPLSWVWFGAVKLERKPLTYEEAHRNLKYHTHSLVKPSSYYYEPLPLPPEDIEPVPEKICIKDEMKADTPSSVDQSPSAVVGGTGRGRGKGTTTRKRKPKNPKTPPVVNTQQQQPQLAQQPQQPQNVQQQQLQQQQQQQQHMQQQHMQQQQMQPNQMGQMPMNMPMNMQQFAPNPNNMMQQNAMLQQQQQQQMQQMGNNPMQQQLNVGGGNGQPNPQMNFMQQGPGGGGAGPQGMPGQQQQWHNAPQQQQPPQPYHNQYAPHQQNMQSNRIERPPLNANSKQALSQMLRQRQPFQQQAQQGPGGGFNPMQQQPQASQQQPGPQQQMNPNQMRQQQMNPQQNPQSVAAFNAMQQQPQQNAQQQQMNPNQQQQQQFMRGGNMRPGMAPNQMNQMNMGGQGMSQNPMMQQQIPQNMVGMVNPNANQMMQSGGAQGGNGVGVGVGVGVGGAGNNPNMGMGGMPQQGMIQQQPQQQPQQQVQFQNFQNQYQQQQQQGMQQQGGGAGVGVGVGMAPNQQQQQQANMMGNFNPQMQQGNRNNPDFMAAAAVAQQQQQQQQQQRVVPGGMMAGNRNQYMNQAPNVTMSTMMGPGPGGVVGQVPPYARQQSAGGGKPGVLNTQQQFQQQQQQQQQLRHQMMQLQGMGGGAGGGMGAGPQQGGGAVGGGAGGGMVPQQQSMNQQQTPNLVAQLQRQNMMGQQQYQPPPY

Target ORF information:

RefSeq Version	NM_080047.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kohtalo (kto), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080047.2

ORF Insert Sequence:

ATGCTGTCCA TGCTGCAGGA GAAGCGTCCG CTGAAGCGCA CCCGTCTGGG TCCGCCGGAC 
ATCTATCCGC AGGACGCGAA GCAGCGAGAG GACGAGCTGA CGCCCACGAA TGTGAAGCAC 
GGATTCACGA CGACGCCACC GCTGTCCGAT GAATTCGGTA CGGCCCACAA CTCGAATGTG 
AACGCCAGCA AAGTGAGCGC ATTCTTTAGC GGCGTCCTGG CCAAGAAGGA GGAGCTGATG 
ACCCTGCCGG ACACGGGGCG CAAGAAGCAG CAGATCAACT GCAAGGACAA CTTCTGGCCC 
GTTTCGCCGC GTCGCAAGTG TACGGTGGAC GCCTGGTTTA AGGACCTCGC CGGTAACAAA 
CCCCTACTTA GCCTAGCCAA ACGGGCTCCA TCGTTCAACA AGAAGGAGGA GATCTTCATT 
ACTCTGTGCG AGAACCAGGT GAACATGCAA CGCGCCACCT GGTTCATCAA GCTGAGCGCC 
GCCTATACGC TTAGCTTCAC CGAGTCTAAG AACAAAAAGC GATCCATTTA CGACCCGGCT 
GCCGAGTGGA CCGGCAACAT GATCAAGTTC ATGAAAGAGC TGCTTCCCAA GCTCCAAGAG 
TATTATCAGC AGAACCACGA CAAGTCCTCT TCGAATGGCA CCACTTCCGG AAGCCTGACC 
GCCGCCGGTA ATGGTCCTGC CAGCAATGGC TCCACTGGCA CCAGCTCTAT TAACTCCGTC 
ACCGGTAGCA GTGCTTCCAC GAACGTAATC CCGGTACCCA GCATGGCCAG TCCACTGCCA 
CCGATTCATA GCCCAGCTAA TGGCCAACAG GCAGCTCCTG GCGGAGGAGT GAATGCCGGC 
AGTGTCATGC CGACGACGGG AAGCCTTGGC GGAGTAGTCG GTGGGCCGGG GTCTTCTGTG 
GTAGGAGGCG CAGCGGGTGC AGGAGCTGCA GTTCCACCAG GCTCTACAAT TTCTGGAATT 
GGAAGCCAGT TCGAAGATAG TCGCAATGCG CTTAAGTATT GGAAATACTG CCACCAATTG 
AGCAAGTACA TGTACGAGGA ATCACTTTTG GACCGACAGG AATTTCTAAA CTGGATCCTC 
GACCTTTTGG ACAAGATGCG TACGCAGGCA AGCTTCGACG AACCGTTGAA GAAATTGGTG 
CTCAGCTTTG CCCTTCAGTA CATGCACGAC TTCGTCCAGT CGGAGCGTCT TTGCAGAAAG 
ATGGCCTACA TAGTGTCCAA AAAACTGGCC CAGTTGCTGA ACACTGTCGT AGAACAGCAG 
ACCATCAAAG AGTTGGACGA GCCGAAGTTG CAACAAGATC CCTATGAGCT GGCGCTTCAG 
GAACAAATGA GTTGTCCCCA TCATCGGGAT ATAGTGCTGT ATTTAAGCAC GATCCTACAG 
ATCATCACAA TCGAGTGCCC CACAGCTCTG GTCTGGAGTG GGATAGCAGC TCACCGAGCA 
CCCTCCTCGT TGTTGGGTAG TCCGCTGGAC CATTTGCCTT TGGCGCCCTC AGTCTTGCCG 
ATGCCCACAC GCTGTCCGCG AACCAATCAC GAGATTCGAC GTCAATTGCG GGCCGCCGAG 
TCGGACATCG TATTGCGCAC TCAGCATGCA GAGCAGCGCT GGTTTGCAGC CAAGTGGCTG 
AGTGCCGGCA AGAATCAATA CACCAGTGTG CTGGCTACAC TGGACCACTT GGACACGCAC 
TGCTTCGATC GCATGGAACA CAATAATAGT ATCGATACCC TATACGCACA AATATTTCCC 
AGCCCAACTG TCAGTCGCAG GCGGGAGGAG GATCAGGTGG AACCTCGTCC GCCTTATGAA 
CCCAAACAAG ACAAGGACAC GGTACGCATT CTGTGCGAGT GGGCTGTGTC GGGGCAGCGT 
TGGGGAGAAC ACAGAGCCAT GGTGGTGGCC ATTCTACTTG ACAAGCGCCA GATCGACGTG 
ACAAGCACGC CGGCGGATCA ACAATCCAGT GATAAGGATG ACAAGGACTC GCTAGCTTCG 
GGCGCCGGCT TAATTGATGG CCTGCCTGTG TTTCAGCACG TGTTAATGCA CTTCCTTGAC 
CACGATGCTC CCGTCCTCGA TGAGCACGTG AGCAGTCCAC AGCAGCGCAC GGAATTTACC 
AACCTTGTTC AGCTGTTCAG TGCATTGATT CGCCACGATG TCTTTTCGCA CAACGCGTAC 
ATGCACACTC TAATTTCACG AGGAGATCTG CTCCTGGAGT CGGTGCTGGT GATCAAATCA 
GGAACAACGG CCACGAAAAC ATCGCCGCCA CCTCCAGCTC CACCACCAAC GACGACGCAC 
GGTTTCGATG ATGACGGATT CGGCGGGGGC CTGGACTTCA AACACAACGA ATTTGATGAT 
TCCAATGTGG ACGACGATTT GGACAAACTG GTGCAAAATA TTAAGGAGAA AGGACAACAG 
CACGAAGCTC CAGACAGTCC CAAGATTGGT CCTCCAGGCG ATGGAGAAAC CAATCCTGGC 
GGTAGCATAT CGCGGCATTA CGTGTACACC AAGCACTTCC CCATCCCCCA GGATGATCCC 
AGCATGAGCA GCTACAGCAG TGAAAGCAAC CAGCGATACA TCCTGCTCTT CGGAGTCGGC 
AAAGAGAGGG ACGAGAAGAA GCATGCTGTC AAGAAGATGT CTAAGGAAAT TGGAAAGCTT 
TTCACAAAAA AGTTTAGCAT AGATGTGGCG GCGGCCGGTC ATGTGAAGAA GCACTCGCGC 
AACGAGTTTA ACTTCGAAGC TACCACGTCC AAGTGCCAGC AGATGGCTTA TTTCGACCAG 
CATGTGGTCA CAGCCCAATG TGCGGCCAAT GTCCTGGAGC AGCTGAACGG ATTCGCGTTA 
GGGAACAACA ACTACCTGCC GGTACAGGAG CACGTGGCGT TCCTCTTTGA TCTGATGGAG 
CTGGCTCTTA ATATCTACAG TCTTTTGGAA CTGTGCGATA GTCTGTTGAA GGAGTTGCCT 
GAGGTGGAGC ACCAGTTGCA GCTAAAGAAG TCAAACCTAG TGCGCAGTTA CACCACTTCA 
CTTGCCCTCT ACATCGTCAG CATCCTGCGG CGCTACCACA GCTGCCTTCT TCTTTCACCG 
GAGCAGACGC TGTCTGTTTT TGAGGGAGTC TGTCGTACCA TTCGGCACGT ATCGAATCCG 
AGTGAGTGCA CTTCGGCGGA GCGCTGCATT ATTGCCTACC TGAGCGACCT ACACGAGTCG 
TGTGTTTTGC TCCAGGGAAA GGAACAATCC ACGGAGTACT ACCAGCAGCT GCAGTGCATC 
AAGCGGTTCA AGGACATATT CAACACTCCA GAGCAGCTTG ACCTACCACC TCAGGGTTAC 
AACCCACTGT TGTTGCAGGA GCTTTTCATG GCCCCTCGAC GCGGTGGTAA ACTGGATCCT 
CACTGGCTGG GAACCCTGCA CGAGTCGCCG GCCAATGTAT ACAGTTTCGT GTCTAACGCA 
TTAATAGCCG TCTGCCGAGA GACGGACAAC GAGCGGCTGA ACGATGTGGC CCTCGCATGT 
GCCGAACTGA CCGCCAGCTG CAACGTCTTG TCTGAGGAGT GGATATACGC ACTGCAGAGT 
CTGTGCAGCG GCAGTAAGAG TCCGCGTTAT CCGCACCTGG GCGGACAGGT CGACATTGGA 
CAACTTAAGA CTCACAACGC CCTGGCCGTG TTCGTATGCA TTCTGGTGGC CAGACATTGC 
TTCTCACTAG CCGACTTTGT AAGTAAGTTC GCCCTGCCTA CATTGGCCCG ATCGGTAAGC 
GCTGGTGGAG CCGAACTGAG TGTAGATGCA GAGGCAGGTG CTCGTCTTAC ATGTCACTTG 
GTGCTGAAGC TGTTTAAGAC CTTGGAGATT CCTCAACCTG GCATGTACTC GGTAAGCACT 
TCCCCGAATC CACTGCACGC TGTGGGAAAC GACTTTAGCA TTCGGCTGAG CTGCGATCGT 
CATCTTCTGG TGGGTGCCCA TAAGACCATT CCCATTGCCG CAGTGCTTGC CGTGCTCAAG 
GCCATTCTCA TTGTTGTGGA CAATGCGGCG CTTAAAACAC CATTGGCTTC CGGCAGCGGC 
ACCTCCTCTG GCGGATTGGG CGGAGCTTTT GGAAGCGGCA AGCGTAGTGG CTTCAATACA 
CCGGTCCATC CGGGAAGCAC ACCCAAGAGC AACGAACAAC GACCGGCCGA TCTCAGCCAG 
ATCCTGGGCA CCAGCGATTT ACAATTGGGC AGCAGTTTGA CAAGCGAACC CGAAGCCCTG 
CAACAGCCAT CGGTCGGAGG AATGGAGCAG ATCTCGCTGT TGGAGTTCGC CCAGGCGGTG 
CTCAAGCAGA TCTGCGCCCA GGAACACGTT CTGGAAAGAT GCCTAAAGAA CGCAGAGCAA 
CTCTGCGACA TGATAATCGA CGAAATGCTG ACCGCGAAAC AGGCACAGCG GGTTCTGCAC 
ATGATCTGCT ATCCGGAGCC TGAGTTTAAC ATCATCTCCG AACTGGATCA GCGCTCAATG 
ATCGTACGCA TACTAGAGAA TCTCGGACAG TGGACACTGC GTATCTCGTG GCTGGATCTT 
CAGCTTATGT ACCGCCAATC GCTGAGCAAC AACGCGGAGC TTAATGTTTG GCTCGACACA 
GTGGCACGCG CGGCAATCGA CGTCTTCCAC ATGGAGGAAG TTGTCCTGCC GGGTGCCGTG 
AAGGCGACCC ACAAACCCAA ACCGTCCACC TGGCTAGTGG CTCCACTCAT CGCTAAACTA 
ACACCAGCGG TGCAGGGCAG GATACTTCGA GTGGCTGGTC AGGTTCTTGA GAGCATGAAC 
TATTTCTCCA AGGTGTCCAA GTCCGATTGC AATAGCTCGG GCAGTGGTGA CGAGCGCGAG 
AAGAGCAACA GTTGTCACAG TAGCAATAGC TATGGACTGG GCGGCGTGCC CGCACGCAAC 
AAGAAGATGC CGCTTGATTA TCAGCCTTTC CTGGGTCTCA TCCTAACTTG CCTGAAAGGC 
CAGGACGAGT ACAAGGAAAA CTTACTGGTT TCGCTCTATG CCCAGTTATC ACAATGTCTG 
CAATCCTTTG CTGAGCTGGA CACAATCGGT GGCATTGATG AGCCGCAGGC GCGCGAGGAG 
ATCCTCGATG CGCTGCAGTT GCGCTTTTCG CTGGTGGGCG GCATGTTCGA GGCTATCCAA 
AAGAACAGTA CCCCCACCAC AGACTGGGCT ATTCTTCTGG CCCAATTGGT TTGCCAGGGC 
GTGGTAGATC TCAGCTGCAA TCGTGAGCTG TTCACCACCG TTGTGGACAT GTTGGCCACA 
TTGGTGCACT CAACGCTGGT CTCTGATGAC GAGAGGCACT ACATGAACCT GATGAAGAAG 
CTAAAGAAGG AAATCGGAGA GAAGAACAAC GCCTCCATTC GGGTTATTCG GCAGCTTCTT 
CCACTTTACA AGCAGCCTAC TGAGGTGATT GCGTGCGAAC ACTCTGGAAT GGACACCAAG 
GGCAACAAGA TCTGCGATAT AGACAAAAAA CAACTACGCA TTTCGGACAA ACAGCGTATC 
AGCGTGTGGG ACATCCTTGA GGGTCACAAG AACCCGGCTC CTTTGTCCTG GGTGTGGTTT 
GGTGCAGTCA AGCTGGAGCG CAAGCCCCTT ACTTACGAGG AGGCTCACCG CAACCTGAAG 
TATCACACGC ACAGTCTGGT CAAGCCCAGT AGTTATTACT ATGAACCGTT GCCACTGCCA 
CCTGAAGACA TTGAGCCTGT TCCCGAGAAG ATTTGCATAA AGGATGAAAT GAAGGCGGAC 
ACGCCCTCGT CGGTGGATCA GTCGCCAAGC GCTGTGGTTG GCGGCACGGG AAGAGGTCGC 
GGCAAGGGAA CGACCACGCG GAAACGCAAG CCGAAGAACC CGAAAACGCC ACCGGTGGTC 
AATACTCAGC AACAGCAGCC TCAGCTGGCA CAGCAGCCTC AACAGCCGCA GAATGTGCAG 
CAACAGCAGT TGCAACAGCA GCAGCAACAA CAGCAGCACA TGCAGCAGCA GCACATGCAA 
CAGCAGCAGA TGCAGCCCAA CCAAATGGGC CAGATGCCAA TGAACATGCC GATGAACATG 
CAGCAGTTTG CACCCAATCC GAACAACATG ATGCAGCAGA ATGCCATGTT ACAACAGCAG 
CAGCAGCAGC AGATGCAGCA AATGGGCAAC AACCCGATGC AGCAGCAGCT GAATGTGGGC 
GGTGGGAACG GGCAGCCAAA TCCCCAGATG AACTTCATGC AGCAGGGACC AGGGGGCGGT 
GGAGCCGGAC CGCAGGGTAT GCCTGGCCAG CAGCAACAGT GGCACAATGC GCCGCAGCAG 
CAGCAGCCGC CACAACCGTA CCACAACCAA TACGCTCCGC ATCAGCAGAA CATGCAGTCA 
AATCGCATCG AGCGCCCGCC GTTGAATGCC AACTCCAAGC AGGCGCTATC ACAGATGCTA 
CGACAACGAC AACCATTCCA GCAGCAAGCG CAACAGGGTC CGGGGGGTGG TTTCAATCCG 
ATGCAGCAAC AACCACAGGC TTCGCAACAA CAGCCCGGTC CTCAGCAGCA GATGAACCCC 
AACCAAATGC GCCAGCAGCA AATGAATCCC CAGCAAAATC CTCAGTCTGT CGCTGCATTC 
AACGCCATGC AGCAGCAGCC GCAGCAGAAC GCCCAGCAGC AACAGATGAA TCCCAACCAG 
CAGCAACAGC AGCAGTTCAT GCGCGGCGGC AACATGCGAC CCGGCATGGC GCCCAACCAG 
ATGAATCAGA TGAACATGGG AGGTCAAGGC ATGTCGCAGA ATCCGATGAT GCAGCAACAA 
ATCCCACAGA ATATGGTGGG CATGGTCAAC CCGAATGCCA ACCAAATGAT GCAGAGCGGT 
GGAGCTCAGG GAGGCAACGG TGTGGGCGTT GGTGTTGGCG TCGGCGTTGG CGGTGCCGGC 
AACAATCCCA ACATGGGCAT GGGAGGCATG CCTCAGCAAG GCATGATACA ACAGCAGCCG 
CAGCAACAGC CGCAGCAGCA GGTGCAGTTC CAGAACTTCC AGAATCAGTA CCAGCAGCAG 
CAACAGCAGG GCATGCAGCA ACAGGGCGGT GGAGCAGGCG TTGGAGTGGG CGTCGGCATG 
GCGCCCAATC AGCAGCAACA GCAGCAGGCC AACATGATGG GCAACTTCAA TCCGCAGATG 
CAGCAGGGGA ATCGCAACAA TCCCGACTTT ATGGCCGCCG CAGCGGTTGC CCAGCAGCAG 
CAACAACAAC AACAGCAACA GCGCGTTGTT CCCGGAGGAA TGATGGCCGG CAACCGGAAC 
CAGTACATGA ACCAGGCGCC CAACGTAACC ATGTCCACCA TGATGGGTCC GGGGCCGGGT 
GGCGTCGTCG GCCAGGTGCC TCCGTACGCC CGTCAGCAGT CGGCGGGAGG AGGCAAACCC 
GGCGTCCTCA ACACCCAGCA GCAGTTCCAG CAGCAGCAAC AGCAACAACA GCAGCTGAGG 
CACCAAATGA TGCAGTTGCA GGGTATGGGT GGTGGGGCAG GCGGCGGCAT GGGAGCCGGG 
CCTCAGCAGG GGGGTGGCGC CGTTGGCGGT GGCGCCGGAG GCGGAATGGT GCCGCAGCAG 
CAGAGCATGA ACCAGCAACA AACGCCGAAT CTGGTGGCTC AGCTCCAGCG GCAGAACATG 
ATGGGCCAGC AGCAGTATCA ACCACCACCG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kto ( NM_001259906.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246835.1 Drosophila melanogaster kohtalo (kto), transcript variant B, mRNA.

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CloneID	OFa19904
Clone ID Related Accession (Same CDS sequence)	NM_001259906.1
Accession Version	NM_001259906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kohtalo, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACATGC AGCAGTTTGC ACCCAATCCG AACAACATGA TGCAGCAGAA TGCCATGTTA 
CAACAGCAGC AGCAGCAGCA GATGCAGCAA ATGGGCAACA ACCCGATGCA GCAGCAGCTG 
AATGTGGGCG GTGGGAACGG GCAGCCAAAT CCCCAGATGA ACTTCATGCA GCAGGGACCA 
GGGGGCGGTG GAGCCGGACC GCAGGGTATG CCTGGCCAGC AGCAACAGTG GCACAATGCG 
CCGCAGCAGC AGCAGCCGCC ACAACCGTAC CACAACCAAT ACGCTCCGCA TCAGCAGAAC 
ATGCAGTCAA ATCGCATCGA GCGCCCGCCG TTGAATGCCA ACTCCAAGCA GGCGCTATCA 
CAGATGCTAC GACAACGACA ACCATTCCAG CAGCAAGCGC AACAGGGTCC GGGGGGTGGT 
TTCAATCCGA TGCAGCAACA ACCACAGGCT TCGCAACAAC AGCCCGGTCC TCAGCAGCAG 
ATGAACCCCA ACCAAATGCG CCAGCAGCAA ATGAATCCCC AGCAAAATCC TCAGTCTGTC 
GCTGCATTCA ACGCCATGCA GCAGCAGCCG CAGCAGAACG CCCAGCAGCA ACAGATGAAT 
CCCAACCAGC AGCAACAGCA GCAGTTCATG CGCGGCGGCA ACATGCGACC CGGCATGGCG 
CCCAACCAGA TGAATCAGAT GAACATGGGA GGTCAAGGCA TGTCGCAGAA TCCGATGATG 
CAGCAACAAA TCCCACAGAA TATGGTGGGC ATGGTCAACC CGAATGCCAA CCAAATGATG 
CAGAGCGGTG GAGCTCAGGG AGGCAACGGT GTGGGCGTTG GTGTTGGCGT CGGCGTTGGC 
GGTGCCGGCA ACAATCCCAA CATGGGCATG GGAGGCATGC CTCAGCAAGG CATGATACAA 
CAGCAGCCGC AGCAACAGCC GCAGCAGCAG GTGCAGTTCC AGAACTTCCA GAATCAGTAC 
CAGCAGCAGC AACAGCAGGG CATGCAGCAA CAGGGCGGTG GAGCAGGCGT TGGAGTGGGC 
GTCGGCATGG CGCCCAATCA GCAGCAACAG CAGCAGGCCA ACATGATGGG CAACTTCAAT 
CCGCAGATGC AGCAGGGGAA TCGCAACAAT CCCGACTTTA TGGCCGCCGC AGCGGTTGCC 
CAGCAGCAGC AACAACAACA ACAGCAACAG CGCGTTGTTC CCGGAGGAAT GATGGCCGGC 
AACCGGAACC AGTACATGAA CCAGGCGCCC AACGTAACCA TGTCCACCAT GATGGGTCCG 
GGGCCGGGTG GCGTCGTCGG CCAGGTGCCT CCGTACGCCC GTCAGCAGTC GGCGGGAGGA 
GGCAAACCCG GCGTCCTCAA CACCCAGCAG CAGTTCCAGC AGCAGCAACA GCAACAACAG 
CAGCTGAGGC ACCAAATGAT GCAGTTGCAG GGTATGGGTG GTGGGGCAGG CGGCGGCATG 
GGAGCCGGGC CTCAGCAGGG GGGTGGCGCC GTTGGCGGTG GCGCCGGAGG CGGAATGGTG 
CCGCAGCAGC AGAGCATGAA CCAGCAACAA ACGCCGAATC TGGTGGCTCA GCTCCAGCGG 
CAGAACATGA TGGGCCAGCA GCAGTATCAA CCACCACCGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246835.1
CDS	37..1641
Translation	MNMQQFAPNPNNMMQQNAMLQQQQQQQMQQMGNNPMQQQLNVGGGNGQPNPQMNFMQQGPGGGGAGPQGMPGQQQQWHNAPQQQQPPQPYHNQYAPHQQNMQSNRIERPPLNANSKQALSQMLRQRQPFQQQAQQGPGGGFNPMQQQPQASQQQPGPQQQMNPNQMRQQQMNPQQNPQSVAAFNAMQQQPQQNAQQQQMNPNQQQQQQFMRGGNMRPGMAPNQMNQMNMGGQGMSQNPMMQQQIPQNMVGMVNPNANQMMQSGGAQGGNGVGVGVGVGVGGAGNNPNMGMGGMPQQGMIQQQPQQQPQQQVQFQNFQNQYQQQQQQGMQQQGGGAGVGVGVGMAPNQQQQQQANMMGNFNPQMQQGNRNNPDFMAAAAVAQQQQQQQQQQRVVPGGMMAGNRNQYMNQAPNVTMSTMMGPGPGGVVGQVPPYARQQSAGGGKPGVLNTQQQFQQQQQQQQQLRHQMMQLQGMGGGAGGGMGAGPQQGGGAVGGGAGGGMVPQQQSMNQQQTPNLVAQLQRQNMMGQQQYQPPPY

Target ORF information:

RefSeq Version	NM_001259906.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kohtalo (kto), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259906.1

ORF Insert Sequence:

ATGAACATGC AGCAGTTTGC ACCCAATCCG AACAACATGA TGCAGCAGAA TGCCATGTTA 
CAACAGCAGC AGCAGCAGCA GATGCAGCAA ATGGGCAACA ACCCGATGCA GCAGCAGCTG 
AATGTGGGCG GTGGGAACGG GCAGCCAAAT CCCCAGATGA ACTTCATGCA GCAGGGACCA 
GGGGGCGGTG GAGCCGGACC GCAGGGTATG CCTGGCCAGC AGCAACAGTG GCACAATGCG 
CCGCAGCAGC AGCAGCCGCC ACAACCGTAC CACAACCAAT ACGCTCCGCA TCAGCAGAAC 
ATGCAGTCAA ATCGCATCGA GCGCCCGCCG TTGAATGCCA ACTCCAAGCA GGCGCTATCA 
CAGATGCTAC GACAACGACA ACCATTCCAG CAGCAAGCGC AACAGGGTCC GGGGGGTGGT 
TTCAATCCGA TGCAGCAACA ACCACAGGCT TCGCAACAAC AGCCCGGTCC TCAGCAGCAG 
ATGAACCCCA ACCAAATGCG CCAGCAGCAA ATGAATCCCC AGCAAAATCC TCAGTCTGTC 
GCTGCATTCA ACGCCATGCA GCAGCAGCCG CAGCAGAACG CCCAGCAGCA ACAGATGAAT 
CCCAACCAGC AGCAACAGCA GCAGTTCATG CGCGGCGGCA ACATGCGACC CGGCATGGCG 
CCCAACCAGA TGAATCAGAT GAACATGGGA GGTCAAGGCA TGTCGCAGAA TCCGATGATG 
CAGCAACAAA TCCCACAGAA TATGGTGGGC ATGGTCAACC CGAATGCCAA CCAAATGATG 
CAGAGCGGTG GAGCTCAGGG AGGCAACGGT GTGGGCGTTG GTGTTGGCGT CGGCGTTGGC 
GGTGCCGGCA ACAATCCCAA CATGGGCATG GGAGGCATGC CTCAGCAAGG CATGATACAA 
CAGCAGCCGC AGCAACAGCC GCAGCAGCAG GTGCAGTTCC AGAACTTCCA GAATCAGTAC 
CAGCAGCAGC AACAGCAGGG CATGCAGCAA CAGGGCGGTG GAGCAGGCGT TGGAGTGGGC 
GTCGGCATGG CGCCCAATCA GCAGCAACAG CAGCAGGCCA ACATGATGGG CAACTTCAAT 
CCGCAGATGC AGCAGGGGAA TCGCAACAAT CCCGACTTTA TGGCCGCCGC AGCGGTTGCC 
CAGCAGCAGC AACAACAACA ACAGCAACAG CGCGTTGTTC CCGGAGGAAT GATGGCCGGC 
AACCGGAACC AGTACATGAA CCAGGCGCCC AACGTAACCA TGTCCACCAT GATGGGTCCG 
GGGCCGGGTG GCGTCGTCGG CCAGGTGCCT CCGTACGCCC GTCAGCAGTC GGCGGGAGGA 
GGCAAACCCG GCGTCCTCAA CACCCAGCAG CAGTTCCAGC AGCAGCAACA GCAACAACAG 
CAGCTGAGGC ACCAAATGAT GCAGTTGCAG GGTATGGGTG GTGGGGCAGG CGGCGGCATG 
GGAGCCGGGC CTCAGCAGGG GGGTGGCGCC GTTGGCGGTG GCGCCGGAGG CGGAATGGTG 
CCGCAGCAGC AGAGCATGAA CCAGCAACAA ACGCCGAATC TGGTGGCTCA GCTCCAGCGG 
CAGAACATGA TGGGCCAGCA GCAGTATCAA CCACCACCGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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kto cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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