g cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	g
Entrez Gene ID	44819
Full Name	garnet
Synonyms	AP-3delta,AP3delta,CG10986,CG11197,Dmel\CG10986,apl5,cg11197,delta,l(1)Mc19
General protein information	Preferred Names garnet Names CG10986 gene product from transcript CG10986-RD AP-3, delta subunit CG10986-PB CG10986-PD CG10986-PF adaptor protein complex 3, delta subunit g-PB g-PD g-PF lethal(1)Mc19 salmon
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12B4-12B4\|1-44.4 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_080046.3	NP_524785.2	garnet, isoform B
NM_001272599.1	NP_001259528.1	garnet, isoform D
NM_001272600.1	NP_001259529.1	garnet, isoform F

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000161	XP_310978.5
Drosophila melanogaster (fruit fly)	g	NP_524785.2
Caenorhabditis elegans (roundworm)	apd-3	NP_494570.1
Xenopus tropicalis (tropical clawed frog)	ap3d1	XP_002937985.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Atg2 and ArfGAP1 may have roles in modifying the eye pigmentation phenotype of AP-3 mutants in Drosophila melanogaster
Title: Identification of Atg2 and ArfGAP1 as Candidate Genetic Modifiers of the Eye Pigmentation Phenotype of Adaptor Protein-3 (AP-3) Mutants in Drosophila melanogaster.

The following g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03739	NM_001272600.1 Latest version!	Drosophila melanogaster garnet (g), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa02355	NM_080046.3 Latest version!	Drosophila melanogaster garnet (g), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa03738	NM_001272599.1 Latest version!	Drosophila melanogaster garnet (g), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

g ( NM_001272600.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259529.1 Drosophila melanogaster garnet (g), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa03739
Clone ID Related Accession (Same CDS sequence)	NM_001272600.1
Accession Version	NM_001272600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	garnet, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGGGCT ACGACATCTC CTGGGCGGGC TTCAATATCA TCGAGGTGAT GAGCTCATCC 
AGATTTACCT GCAAGCGAAT TGGCTATCTG GCGGCTAGCC AATGTTTCCA TCCGGACAGC 
GAGCTGCTCA TGCTGACCAC GAATATGATA CGCAAGGATC TCAACTCACA GAATCAATAC 
GATGCGGGTG TGGCGCTCAG TGGACTGAGC TGCTTTATAT CGCCCGATCT GTCCCGCGAC 
TTGGCCAATG ATATTATGAC ACTGATGAGT TCAACAAAAC CGTATCTGCG CATGAAAGCC 
GTCCTTATGA TGTATAAAGT ATTTCTACGC TATCCCGAGG CATTGAGACC GGCATTTCCC 
AAACTCAAAG AGAAACTTGA GGATCCTGAT CCAGGTGTTC AATCAGCTGC CGTGAATGTG 
ATTTGTGAAC TAGCACGTAA GAATCCAAAA AACTACCTGC CCTTGGCGCC GATCTTCTTC 
AAGCTAATGA CCACATCGAC CAACAATTGG ATGCTGATCA AGATCATCAA GCTGTTTGGC 
GCTTTAACCC CCTTAGAACC CCGGCTAGGA AAGAAATTAA TAGAACCACT CACAAATCTA 
ATTCATAGCA CCTCGGCGAT GAGTCTCCTG TATGAGTGCA TCAACACGGT GATCGCGGTG 
CTCATCAGCA TTAGCAGCGG CATGCCCAAC CACAGTGCCT CCATTCAGCT CTGCGTGCAG 
AAGCTGCGCA TCCTCATCGA GGACTCGGAC CAGAACTTGA AATATCTGGG ACTGTTGGCC 
ATGTCGAAAA TCCTAAAGAC GCACCCGAAG AGTGTGCAGG CGCACAAGGA TCTCATACTC 
GCCTGCCTGG ACGACAAGGA CGAATCGATA CGTCTGCGCG CCCTGGACCT GCTCTACGGC 
ATGGTCTCGA AGAAGAACCT TATGGAGATT GTTAAGCGAT TGCTGGGCCA CATGGAGCGG 
GCAGAGGGTT CGGCCTATCG GGACGAATTG CTTTACAAGG TGATCGAGAT ATGCGCGCAG 
AGTTCGTATC TGTATGTGAC CAACTTCGAG TGGTATCTAA CTGTGCTCGT CGAGCTCATT 
CAACTTGAGG CTGGCTCACG GCACGGTCGC CTTATAGCAG AGCAACTACT TGACGTGGCC 
ATTCGAGTGC CTGTTGTTCG GCAATTTGCC GTCAACGAGA TGACCAATCT GCTGGACACG 
TTCACCGTTT CGGCGCAAAG TAATTCCATG TACGAGGTGC TCTACGCCGC CGCCTGGATC 
GTTGGCGAGT TTGCTGGCGA ACTGGAGGAT GCGGAGAAGA CGCTCAACAT CCTGCTCCGT 
CCGAGGCTAT TGCCCGGTCA CATCCAGGGT GTCTATGTGC AGAACGTGAT TAAGCTGTTC 
GCTCGCCTGG CCACCACGTG TTTAGAGCTT CAGGATCTGC CTGGCTTGGT GACACTCTGC 
GACCATGTGT TGGACAAGCT TCAGCACTTC AATGGTTCCA GCGACATTGA GGTGCAAGAG 
CGAGCCAATT CCGCTTGCAT GCTGATCGAG ATGCTGCGCA ACCAGCTGTC CACGTCGACC 
GATGCCATGG CCATGGACAC GACGACGGAG GGCGGCATTC CGCCGCTGGC CATTGAGATT 
GTCCAGGAGA TGACGCTGCT GTTCACAGGC GAGCTAATAC CGGTGGCGCC CAAGGCGCAA 
CGGAAGGTGC CCCTGCCAGA TGGTCTCGAT CTGGACGAGT GGATTAATGC ACCGCCACCG 
GAAGATGCGG CAAGCAGTTC ATCCTCGGAG CACGACAAGG ACGAGCTATT CGTTAGTGCC 
ACGCAGGCAG GAACGGGAGC TGATGGCGGC GAAAAGCGGC GACAGAGTCT CGAGCTCACG 
CCTGAGCAAC TGGAGCGGCA GCGAATGGCT CGTCTCATTG AGCAGTCGAA CAATCCTCAC 
TACCTCAAGT CCACGCCCAC GGCGAGTGGT GCCTCGAATG CGGATCAGTA TGACAATATT 
GATGACATAC CCATTACGGA GCTGCCACTG GACATGGAGG GCGTGGCCGC ACTGCGCGTG 
GGCATCACCA AGCGGTCGGA TAAGTACCTG CAGGAACAGC AGGCGGCGCA GGGCAGCAAG 
GATGGCAAGA AGAAGCATAA AAAGGGTAAA AAGAGCAAAA AGGCCAAAAA TAAAGTGGCA 
TACAACAGCA GCTCCGAATC GGAAGGTGAA CCGAAACCCT TGCACATTGT GAACACCACG 
CTAGACATGC CCGAAGGTGT CTCCATGTCG GACAGCGAGG ACAAGGATGG CAAATACGAT 
CCAAATGATC CGCATCGAGC CCTGGACATT GAATTGGACA TTACGGAGTT CGAGGCGCCC 
GCGGTGAGGT CAGCGTCCAA AAAGTCAGCG GCGGACAAAG AGAATCTCAA GACGCCGGCG 
GATTCGGCCG GCGGTGGTAA CGCCGCCAAG AAGGATAGGA AAAAGGACAA GGACAAGGAC 
AAGGAGAGGA AGGTGAAGCG GGAGCATCGG GAGAGCAAGC GGGAACGCAA GGAGGCTGCT 
GTTCAGTCGG TGATCGATCT CATTGACGCC GACACGCCCA CTCCCAGTCC CAGCCACATC 
TCCGCCACCA GCAACAACAA CAACACTTCA ACTGTCCTTC CAGATGCGCC CAAGCATCAC 
AAAAAGAAGA AGAACAAGGA GAAGACGACG GATGAAGCGC CTGATGCACT GGCCACCGCC 
ACCGGGAGCA GCATCATCGA TGTGGGCGGC GAGGAGGCAT CCGAGGTGGC GTCCAAGGTG 
CACAAGAAGA AGCATAAAAA GGAGAAGTCA CAGCGCAAGG AGAAGAAGAA GGCTTCAGAA 
TCTGCATCCG TATCCGCCAT CGTTTCGATT GGCGACTACG AACAGCCACT GGGAATCTCA 
ACGCCCAGCA AAGAGATTTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259529.1
CDS	262..3165
Translation	MLGYDISWAGFNIIEVMSSSRFTCKRIGYLAASQCFHPDSELLMLTTNMIRKDLNSQNQYDAGVALSGLSCFISPDLSRDLANDIMTLMSSTKPYLRMKAVLMMYKVFLRYPEALRPAFPKLKEKLEDPDPGVQSAAVNVICELARKNPKNYLPLAPIFFKLMTTSTNNWMLIKIIKLFGALTPLEPRLGKKLIEPLTNLIHSTSAMSLLYECINTVIAVLISISSGMPNHSASIQLCVQKLRILIEDSDQNLKYLGLLAMSKILKTHPKSVQAHKDLILACLDDKDESIRLRALDLLYGMVSKKNLMEIVKRLLGHMERAEGSAYRDELLYKVIEICAQSSYLYVTNFEWYLTVLVELIQLEAGSRHGRLIAEQLLDVAIRVPVVRQFAVNEMTNLLDTFTVSAQSNSMYEVLYAAAWIVGEFAGELEDAEKTLNILLRPRLLPGHIQGVYVQNVIKLFARLATTCLELQDLPGLVTLCDHVLDKLQHFNGSSDIEVQERANSACMLIEMLRNQLSTSTDAMAMDTTTEGGIPPLAIEIVQEMTLLFTGELIPVAPKAQRKVPLPDGLDLDEWINAPPPEDAASSSSSEHDKDELFVSATQAGTGADGGEKRRQSLELTPEQLERQRMARLIEQSNNPHYLKSTPTASGASNADQYDNIDDIPITELPLDMEGVAALRVGITKRSDKYLQEQQAAQGSKDGKKKHKKGKKSKKAKNKVAYNSSSESEGEPKPLHIVNTTLDMPEGVSMSDSEDKDGKYDPNDPHRALDIELDITEFEAPAVRSASKKSAADKENLKTPADSAGGGNAAKKDRKKDKDKDKERKVKREHRESKRERKEAAVQSVIDLIDADTPTPSPSHISATSNNNNTSTVLPDAPKHHKKKKNKEKTTDEAPDALATATGSSIIDVGGEEASEVASKVHKKKHKKEKSQRKEKKKASESASVSAIVSIGDYEQPLGISTPSKEIL

Target ORF information:

RefSeq Version	NM_001272600.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster garnet (g), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272600.1

ORF Insert Sequence:

ATGCTGGGCT ACGACATCTC CTGGGCGGGC TTCAATATCA TCGAGGTGAT GAGCTCATCC 
AGATTTACCT GCAAGCGAAT TGGCTATCTG GCGGCTAGCC AATGTTTCCA TCCGGACAGC 
GAGCTGCTCA TGCTGACCAC GAATATGATA CGCAAGGATC TCAACTCACA GAATCAATAC 
GATGCGGGTG TGGCGCTCAG TGGACTGAGC TGCTTTATAT CGCCCGATCT GTCCCGCGAC 
TTGGCCAATG ATATTATGAC ACTGATGAGT TCAACAAAAC CGTATCTGCG CATGAAAGCC 
GTCCTTATGA TGTATAAAGT ATTTCTACGC TATCCCGAGG CATTGAGACC GGCATTTCCC 
AAACTCAAAG AGAAACTTGA GGATCCTGAT CCAGGTGTTC AATCAGCTGC CGTGAATGTG 
ATTTGTGAAC TAGCACGTAA GAATCCAAAA AACTACCTGC CCTTGGCGCC GATCTTCTTC 
AAGCTAATGA CCACATCGAC CAACAATTGG ATGCTGATCA AGATCATCAA GCTGTTTGGC 
GCTTTAACCC CCTTAGAACC CCGGCTAGGA AAGAAATTAA TAGAACCACT CACAAATCTA 
ATTCATAGCA CCTCGGCGAT GAGTCTCCTG TATGAGTGCA TCAACACGGT GATCGCGGTG 
CTCATCAGCA TTAGCAGCGG CATGCCCAAC CACAGTGCCT CCATTCAGCT CTGCGTGCAG 
AAGCTGCGCA TCCTCATCGA GGACTCGGAC CAGAACTTGA AATATCTGGG ACTGTTGGCC 
ATGTCGAAAA TCCTAAAGAC GCACCCGAAG AGTGTGCAGG CGCACAAGGA TCTCATACTC 
GCCTGCCTGG ACGACAAGGA CGAATCGATA CGTCTGCGCG CCCTGGACCT GCTCTACGGC 
ATGGTCTCGA AGAAGAACCT TATGGAGATT GTTAAGCGAT TGCTGGGCCA CATGGAGCGG 
GCAGAGGGTT CGGCCTATCG GGACGAATTG CTTTACAAGG TGATCGAGAT ATGCGCGCAG 
AGTTCGTATC TGTATGTGAC CAACTTCGAG TGGTATCTAA CTGTGCTCGT CGAGCTCATT 
CAACTTGAGG CTGGCTCACG GCACGGTCGC CTTATAGCAG AGCAACTACT TGACGTGGCC 
ATTCGAGTGC CTGTTGTTCG GCAATTTGCC GTCAACGAGA TGACCAATCT GCTGGACACG 
TTCACCGTTT CGGCGCAAAG TAATTCCATG TACGAGGTGC TCTACGCCGC CGCCTGGATC 
GTTGGCGAGT TTGCTGGCGA ACTGGAGGAT GCGGAGAAGA CGCTCAACAT CCTGCTCCGT 
CCGAGGCTAT TGCCCGGTCA CATCCAGGGT GTCTATGTGC AGAACGTGAT TAAGCTGTTC 
GCTCGCCTGG CCACCACGTG TTTAGAGCTT CAGGATCTGC CTGGCTTGGT GACACTCTGC 
GACCATGTGT TGGACAAGCT TCAGCACTTC AATGGTTCCA GCGACATTGA GGTGCAAGAG 
CGAGCCAATT CCGCTTGCAT GCTGATCGAG ATGCTGCGCA ACCAGCTGTC CACGTCGACC 
GATGCCATGG CCATGGACAC GACGACGGAG GGCGGCATTC CGCCGCTGGC CATTGAGATT 
GTCCAGGAGA TGACGCTGCT GTTCACAGGC GAGCTAATAC CGGTGGCGCC CAAGGCGCAA 
CGGAAGGTGC CCCTGCCAGA TGGTCTCGAT CTGGACGAGT GGATTAATGC ACCGCCACCG 
GAAGATGCGG CAAGCAGTTC ATCCTCGGAG CACGACAAGG ACGAGCTATT CGTTAGTGCC 
ACGCAGGCAG GAACGGGAGC TGATGGCGGC GAAAAGCGGC GACAGAGTCT CGAGCTCACG 
CCTGAGCAAC TGGAGCGGCA GCGAATGGCT CGTCTCATTG AGCAGTCGAA CAATCCTCAC 
TACCTCAAGT CCACGCCCAC GGCGAGTGGT GCCTCGAATG CGGATCAGTA TGACAATATT 
GATGACATAC CCATTACGGA GCTGCCACTG GACATGGAGG GCGTGGCCGC ACTGCGCGTG 
GGCATCACCA AGCGGTCGGA TAAGTACCTG CAGGAACAGC AGGCGGCGCA GGGCAGCAAG 
GATGGCAAGA AGAAGCATAA AAAGGGTAAA AAGAGCAAAA AGGCCAAAAA TAAAGTGGCA 
TACAACAGCA GCTCCGAATC GGAAGGTGAA CCGAAACCCT TGCACATTGT GAACACCACG 
CTAGACATGC CCGAAGGTGT CTCCATGTCG GACAGCGAGG ACAAGGATGG CAAATACGAT 
CCAAATGATC CGCATCGAGC CCTGGACATT GAATTGGACA TTACGGAGTT CGAGGCGCCC 
GCGGTGAGGT CAGCGTCCAA AAAGTCAGCG GCGGACAAAG AGAATCTCAA GACGCCGGCG 
GATTCGGCCG GCGGTGGTAA CGCCGCCAAG AAGGATAGGA AAAAGGACAA GGACAAGGAC 
AAGGAGAGGA AGGTGAAGCG GGAGCATCGG GAGAGCAAGC GGGAACGCAA GGAGGCTGCT 
GTTCAGTCGG TGATCGATCT CATTGACGCC GACACGCCCA CTCCCAGTCC CAGCCACATC 
TCCGCCACCA GCAACAACAA CAACACTTCA ACTGTCCTTC CAGATGCGCC CAAGCATCAC 
AAAAAGAAGA AGAACAAGGA GAAGACGACG GATGAAGCGC CTGATGCACT GGCCACCGCC 
ACCGGGAGCA GCATCATCGA TGTGGGCGGC GAGGAGGCAT CCGAGGTGGC GTCCAAGGTG 
CACAAGAAGA AGCATAAAAA GGAGAAGTCA CAGCGCAAGG AGAAGAAGAA GGCTTCAGAA 
TCTGCATCCG TATCCGCCAT CGTTTCGATT GGCGACTACG AACAGCCACT GGGAATCTCA 
ACGCCCAGCA AAGAGATTTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

g ( NM_080046.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524785.2 Drosophila melanogaster garnet (g), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02355
Clone ID Related Accession (Same CDS sequence)	NM_080046.3
Accession Version	NM_080046.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	garnet, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_080046.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACTGA AAAAAGTGAA GGGTAACTTT TTCGAGCGTA TGTTTGATAA AAACCTCACC 
GATTTGGTGC GCGGCATTAG GAACAACAAG GATAATGAGG CCAAATACAT ATCGACGTGC 
ATTGAGGAGA TCAAACAGGA GCTGCGACAG GACAATATAA GTGTCAAGTG TAATGCAGTG 
GCCAAGTTGA CCTACATACA AATGCTGGGC TACGACATCT CCTGGGCGGG CTTCAATATC 
ATCGAGGTGA TGAGCTCATC CAGATTTACC TGCAAGCGAA TTGGCTATCT GGCGGCTAGC 
CAATGTTTCC ATCCGGACAG CGAGCTGCTC ATGCTGACCA CGAATATGAT ACGCAAGGAT 
CTCAACTCAC AGAATCAATA CGATGCGGGT GTGGCGCTCA GTGGACTGAG CTGCTTTATA 
TCGCCCGATC TGTCCCGCGA CTTGGCCAAT GATATTATGA CACTGATGAG TTCAACAAAA 
CCGTATCTGC GCATGAAAGC CGTCCTTATG ATGTATAAAG TATTTCTACG CTATCCCGAG 
GCATTGAGAC CGGCATTTCC CAAACTCAAA GAGAAACTTG AGGATCCTGA TCCAGGTGTT 
CAATCAGCTG CCGTGAATGT GATTTGTGAA CTAGCACGTA AGAATCCAAA AAACTACCTG 
CCCTTGGCGC CGATCTTCTT CAAGCTAATG ACCACATCGA CCAACAATTG GATGCTGATC 
AAGATCATCA AGCTGTTTGG CGCTTTAACC CCCTTAGAAC CCCGGCTAGG AAAGAAATTA 
ATAGAACCAC TCACAAATCT AATTCATAGC ACCTCGGCGA TGAGTCTCCT GTATGAGTGC 
ATCAACACGG TGATCGCGGT GCTCATCAGC ATTAGCAGCG GCATGCCCAA CCACAGTGCC 
TCCATTCAGC TCTGCGTGCA GAAGCTGCGC ATCCTCATCG AGGACTCGGA CCAGAACTTG 
AAATATCTGG GACTGTTGGC CATGTCGAAA ATCCTAAAGA CGCACCCGAA GAGTGTGCAG 
GCGCACAAGG ATCTCATACT CGCCTGCCTG GACGACAAGG ACGAATCGAT ACGTCTGCGC 
GCCCTGGACC TGCTCTACGG CATGGTCTCG AAGAAGAACC TTATGGAGAT TGTTAAGCGA 
TTGCTGGGCC ACATGGAGCG GGCAGAGGGT TCGGCCTATC GGGACGAATT GCTTTACAAG 
GTGATCGAGA TATGCGCGCA GAGTTCGTAT CTGTATGTGA CCAACTTCGA GTGGTATCTA 
ACTGTGCTCG TCGAGCTCAT TCAACTTGAG GCTGGCTCAC GGCACGGTCG CCTTATAGCA 
GAGCAACTAC TTGACGTGGC CATTCGAGTG CCTGTTGTTC GGCAATTTGC CGTCAACGAG 
ATGACCAATC TGCTGGACAC GTTCACCGTT TCGGCGCAAA GTAATTCCAT GTACGAGGTG 
CTCTACGCCG CCGCCTGGAT CGTTGGCGAG TTTGCTGGCG AACTGGAGGA TGCGGAGAAG 
ACGCTCAACA TCCTGCTCCG TCCGAGGCTA TTGCCCGGTC ACATCCAGGG TGTCTATGTG 
CAGAACGTGA TTAAGCTGTT CGCTCGCCTG GCCACCACGT GTTTAGAGCT TCAGGATCTG 
CCTGGCTTGG TGACACTCTG CGACCATGTG TTGGACAAGC TTCAGCACTT CAATGGTTCC 
AGCGACATTG AGGTGCAAGA GCGAGCCAAT TCCGCTTGCA TGCTGATCGA GATGCTGCGC 
AACCAGCTGT CCACGTCGAC CGATGCCATG GCCATGGACA CGACGACGGA GGGCGGCATT 
CCGCCGCTGG CCATTGAGAT TGTCCAGGAG ATGACGCTGC TGTTCACAGG CGAGCTAATA 
CCGGTGGCGC CCAAGGCGCA ACGGAAGGTG CCCCTGCCAG ATGGTCTCGA TCTGGACGAG 
TGGATTAATG CACCGCCACC GGAAGATGCG GCAAGCAGTT CATCCTCGGA GCACGACAAG 
GACGAGCTAT TCGTTAGTGC CACGCAGGCA GGAACGGGAG CTGATGGCGG CGAAAAGCGG 
CGACAGAGTC TCGAGCTCAC GCCTGAGCAA CTGGAGCGGC AGCGAATGGC TCGTCTCATT 
GAGCAGTCGA ACAATCCTCA CTACCTCAAG TCCACGCCCA CGGCGAGTGG TGCCTCGAAT 
GCGGATCAGT ATGACAATAT TGATGACATA CCCATTACGG AGCTGCCACT GGACATGGAG 
GGCGTGGCCG CACTGCGCGT GGGCATCACC AAGCGGTCGG ATAAGTACCT GCAGGAACAG 
CAGGCGGCGC AGGGCAGCAA GGATGGCAAG AAGAAGCATA AAAAGGGTAA AAAGAGCAAA 
AAGGCCAAAA ATAAAGTGGC ATACAACAGC AGCTCCGAAT CGGAAGGTGA ACCGAAACCC 
TTGCACATTG TGAACACCAC GCTAGACATG CCCGAAGGTG TCTCCATGTC GGACAGCGAG 
GACAAGGATG GCAAATACGA TCCAAATGAT CCGCATCGAG CCCTGGACAT TGAATTGGAC 
ATTACGGAGT TCGAGGCGCC CGCGGTGAGG TCAGCGTCCA AAAAGTCAGC GGCGGACAAA 
GAGAATCTCA AGACGCCGGC GGATTCGGCC GGCGGTGGTA ACGCCGCCAA GAAGGATAGG 
AAAAAGGACA AGGACAAGGA CAAGGAGAGG AAGGTGAAGC GGGAGCATCG GGAGAGCAAG 
CGGGAACGCA AGGAGGCTGC TGTTCAGTCG GTGATCGATC TCATTGACGC CGACACGCCC 
ACTCCCAGTC CCAGCCACAT CTCCGCCACC AGCAACAACA ACAACACTTC AACTGTCCTT 
CCAGATGCGC CCAAGCATCA CAAAAAGAAG AAGAACAAGG AGAAGACGAC GGATGAAGCG 
CCTGATGCAC TGGCCACCGC CACCGGGAGC AGCATCATCG ATGTGGGCGG CGAGGAGGCA 
TCCGAGGTGG CGTCCAAGGT GCACAAGAAG AAGCATAAAA AGGAGAAGTC ACAGCGCAAG 
GAGAAGAAGA AGGCTTCAGA ATCTGCATCC GTATCCGCCA TCGTTTCGAT TGGCGACTAC 
GAACAGCCAC TGGGAATCTC AACGCCCAGC AAAGAGATTT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524785.2
CDS	239..3343
Translation	MALKKVKGNFFERMFDKNLTDLVRGIRNNKDNEAKYISTCIEEIKQELRQDNISVKCNAVAKLTYIQMLGYDISWAGFNIIEVMSSSRFTCKRIGYLAASQCFHPDSELLMLTTNMIRKDLNSQNQYDAGVALSGLSCFISPDLSRDLANDIMTLMSSTKPYLRMKAVLMMYKVFLRYPEALRPAFPKLKEKLEDPDPGVQSAAVNVICELARKNPKNYLPLAPIFFKLMTTSTNNWMLIKIIKLFGALTPLEPRLGKKLIEPLTNLIHSTSAMSLLYECINTVIAVLISISSGMPNHSASIQLCVQKLRILIEDSDQNLKYLGLLAMSKILKTHPKSVQAHKDLILACLDDKDESIRLRALDLLYGMVSKKNLMEIVKRLLGHMERAEGSAYRDELLYKVIEICAQSSYLYVTNFEWYLTVLVELIQLEAGSRHGRLIAEQLLDVAIRVPVVRQFAVNEMTNLLDTFTVSAQSNSMYEVLYAAAWIVGEFAGELEDAEKTLNILLRPRLLPGHIQGVYVQNVIKLFARLATTCLELQDLPGLVTLCDHVLDKLQHFNGSSDIEVQERANSACMLIEMLRNQLSTSTDAMAMDTTTEGGIPPLAIEIVQEMTLLFTGELIPVAPKAQRKVPLPDGLDLDEWINAPPPEDAASSSSSEHDKDELFVSATQAGTGADGGEKRRQSLELTPEQLERQRMARLIEQSNNPHYLKSTPTASGASNADQYDNIDDIPITELPLDMEGVAALRVGITKRSDKYLQEQQAAQGSKDGKKKHKKGKKSKKAKNKVAYNSSSESEGEPKPLHIVNTTLDMPEGVSMSDSEDKDGKYDPNDPHRALDIELDITEFEAPAVRSASKKSAADKENLKTPADSAGGGNAAKKDRKKDKDKDKERKVKREHRESKRERKEAAVQSVIDLIDADTPTPSPSHISATSNNNNTSTVLPDAPKHHKKKKNKEKTTDEAPDALATATGSSIIDVGGEEASEVASKVHKKKHKKEKSQRKEKKKASESASVSAIVSIGDYEQPLGISTPSKEIL

Target ORF information:

RefSeq Version	NM_080046.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster garnet (g), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080046.3

ORF Insert Sequence:

ATGGCACTGA AAAAAGTGAA GGGTAACTTT TTCGAGCGTA TGTTTGATAA AAACCTCACC 
GATTTGGTGC GCGGCATTAG GAACAACAAG GATAATGAGG CCAAATACAT ATCGACGTGC 
ATTGAGGAGA TCAAACAGGA GCTGCGACAG GACAATATAA GTGTCAAGTG TAATGCAGTG 
GCCAAGTTGA CCTACATACA AATGCTGGGC TACGACATCT CCTGGGCGGG CTTCAATATC 
ATCGAGGTGA TGAGCTCATC CAGATTTACC TGCAAGCGAA TTGGCTATCT GGCGGCTAGC 
CAATGTTTCC ATCCGGACAG CGAGCTGCTC ATGCTGACCA CGAATATGAT ACGCAAGGAT 
CTCAACTCAC AGAATCAATA CGATGCGGGT GTGGCGCTCA GTGGACTGAG CTGCTTTATA 
TCGCCCGATC TGTCCCGCGA CTTGGCCAAT GATATTATGA CACTGATGAG TTCAACAAAA 
CCGTATCTGC GCATGAAAGC CGTCCTTATG ATGTATAAAG TATTTCTACG CTATCCCGAG 
GCATTGAGAC CGGCATTTCC CAAACTCAAA GAGAAACTTG AGGATCCTGA TCCAGGTGTT 
CAATCAGCTG CCGTGAATGT GATTTGTGAA CTAGCACGTA AGAATCCAAA AAACTACCTG 
CCCTTGGCGC CGATCTTCTT CAAGCTAATG ACCACATCGA CCAACAATTG GATGCTGATC 
AAGATCATCA AGCTGTTTGG CGCTTTAACC CCCTTAGAAC CCCGGCTAGG AAAGAAATTA 
ATAGAACCAC TCACAAATCT AATTCATAGC ACCTCGGCGA TGAGTCTCCT GTATGAGTGC 
ATCAACACGG TGATCGCGGT GCTCATCAGC ATTAGCAGCG GCATGCCCAA CCACAGTGCC 
TCCATTCAGC TCTGCGTGCA GAAGCTGCGC ATCCTCATCG AGGACTCGGA CCAGAACTTG 
AAATATCTGG GACTGTTGGC CATGTCGAAA ATCCTAAAGA CGCACCCGAA GAGTGTGCAG 
GCGCACAAGG ATCTCATACT CGCCTGCCTG GACGACAAGG ACGAATCGAT ACGTCTGCGC 
GCCCTGGACC TGCTCTACGG CATGGTCTCG AAGAAGAACC TTATGGAGAT TGTTAAGCGA 
TTGCTGGGCC ACATGGAGCG GGCAGAGGGT TCGGCCTATC GGGACGAATT GCTTTACAAG 
GTGATCGAGA TATGCGCGCA GAGTTCGTAT CTGTATGTGA CCAACTTCGA GTGGTATCTA 
ACTGTGCTCG TCGAGCTCAT TCAACTTGAG GCTGGCTCAC GGCACGGTCG CCTTATAGCA 
GAGCAACTAC TTGACGTGGC CATTCGAGTG CCTGTTGTTC GGCAATTTGC CGTCAACGAG 
ATGACCAATC TGCTGGACAC GTTCACCGTT TCGGCGCAAA GTAATTCCAT GTACGAGGTG 
CTCTACGCCG CCGCCTGGAT CGTTGGCGAG TTTGCTGGCG AACTGGAGGA TGCGGAGAAG 
ACGCTCAACA TCCTGCTCCG TCCGAGGCTA TTGCCCGGTC ACATCCAGGG TGTCTATGTG 
CAGAACGTGA TTAAGCTGTT CGCTCGCCTG GCCACCACGT GTTTAGAGCT TCAGGATCTG 
CCTGGCTTGG TGACACTCTG CGACCATGTG TTGGACAAGC TTCAGCACTT CAATGGTTCC 
AGCGACATTG AGGTGCAAGA GCGAGCCAAT TCCGCTTGCA TGCTGATCGA GATGCTGCGC 
AACCAGCTGT CCACGTCGAC CGATGCCATG GCCATGGACA CGACGACGGA GGGCGGCATT 
CCGCCGCTGG CCATTGAGAT TGTCCAGGAG ATGACGCTGC TGTTCACAGG CGAGCTAATA 
CCGGTGGCGC CCAAGGCGCA ACGGAAGGTG CCCCTGCCAG ATGGTCTCGA TCTGGACGAG 
TGGATTAATG CACCGCCACC GGAAGATGCG GCAAGCAGTT CATCCTCGGA GCACGACAAG 
GACGAGCTAT TCGTTAGTGC CACGCAGGCA GGAACGGGAG CTGATGGCGG CGAAAAGCGG 
CGACAGAGTC TCGAGCTCAC GCCTGAGCAA CTGGAGCGGC AGCGAATGGC TCGTCTCATT 
GAGCAGTCGA ACAATCCTCA CTACCTCAAG TCCACGCCCA CGGCGAGTGG TGCCTCGAAT 
GCGGATCAGT ATGACAATAT TGATGACATA CCCATTACGG AGCTGCCACT GGACATGGAG 
GGCGTGGCCG CACTGCGCGT GGGCATCACC AAGCGGTCGG ATAAGTACCT GCAGGAACAG 
CAGGCGGCGC AGGGCAGCAA GGATGGCAAG AAGAAGCATA AAAAGGGTAA AAAGAGCAAA 
AAGGCCAAAA ATAAAGTGGC ATACAACAGC AGCTCCGAAT CGGAAGGTGA ACCGAAACCC 
TTGCACATTG TGAACACCAC GCTAGACATG CCCGAAGGTG TCTCCATGTC GGACAGCGAG 
GACAAGGATG GCAAATACGA TCCAAATGAT CCGCATCGAG CCCTGGACAT TGAATTGGAC 
ATTACGGAGT TCGAGGCGCC CGCGGTGAGG TCAGCGTCCA AAAAGTCAGC GGCGGACAAA 
GAGAATCTCA AGACGCCGGC GGATTCGGCC GGCGGTGGTA ACGCCGCCAA GAAGGATAGG 
AAAAAGGACA AGGACAAGGA CAAGGAGAGG AAGGTGAAGC GGGAGCATCG GGAGAGCAAG 
CGGGAACGCA AGGAGGCTGC TGTTCAGTCG GTGATCGATC TCATTGACGC CGACACGCCC 
ACTCCCAGTC CCAGCCACAT CTCCGCCACC AGCAACAACA ACAACACTTC AACTGTCCTT 
CCAGATGCGC CCAAGCATCA CAAAAAGAAG AAGAACAAGG AGAAGACGAC GGATGAAGCG 
CCTGATGCAC TGGCCACCGC CACCGGGAGC AGCATCATCG ATGTGGGCGG CGAGGAGGCA 
TCCGAGGTGG CGTCCAAGGT GCACAAGAAG AAGCATAAAA AGGAGAAGTC ACAGCGCAAG 
GAGAAGAAGA AGGCTTCAGA ATCTGCATCC GTATCCGCCA TCGTTTCGAT TGGCGACTAC 
GAACAGCCAC TGGGAATCTC AACGCCCAGC AAAGAGATTT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

g ( NM_001272599.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259528.1 Drosophila melanogaster garnet (g), transcript variant D, mRNA.

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CloneID	OFa03738
Clone ID Related Accession (Same CDS sequence)	NM_001272599.1
Accession Version	NM_001272599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	garnet, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACTGA AAAAAGTGAA GGGTAACTTT TTCGAGCGTA TGTTTGATAA AAACCTCACC 
GATTTGGTGC GCGGCATTAG GAACAACAAG GATAATGAGG CCAAATACAT ATCGACGTGC 
ATTGAGGAGA TCAAACAGGA GCTGCGACAG GACAATATAA GTGTCAAGTG TAATGCAGTG 
GCCAAGTTGA CCTACATACA AATGCTGGGC TACGACATCT CCTGGGCGGG CTTCAATATC 
ATCGAGGTGA TGAGCTCATC CAGATTTACC TGCAAGCGAA TTGGCTATCT GGCGGCTAGC 
CAATGTTTCC ATCCGGACAG CGAGCTGCTC ATGCTGACCA CGAATATGAT ACGCAAGGAT 
CTCAACTCAC AGAATCAATA CGATGCGGGT GTGGCGCTCA GTGGACTGAG CTGCTTTATA 
TCGCCCGATC TGTCCCGCGA CTTGGCCAAT GATATTATGA CACTGATGAG TTCAACAAAA 
CCGTATCTGC GCATGAAAGC CGTCCTTATG ATGTATAAAG TATTTCTACG CTATCCCGAG 
GCATTGAGAC CGGCATTTCC CAAACTCAAA GAGAAACTTG AGGATCCTGA TCCAGGTGTT 
CAATCAGCTG CCGTGAATGT GATTTGTGAA CTAGCACGTA AGAATCCAAA AAACTACCTG 
CCCTTGGCGC CGATCTTCTT CAAGCTAATG ACCACATCGA CCAACAATTG GATGCTGATC 
AAGATCATCA AGCTGTTCGC GAGCCTCACG ACAATTGAAC CGGCGCTGGG ACGCAAATTG 
ACTCAGCCCC TAATTGAAAT TATTTCCAGC ACCTCGGCGA TGAGTCTCCT GTATGAGTGC 
ATCAACACGG TGATCGCGGT GCTCATCAGC ATTAGCAGCG GCATGCCCAA CCACAGTGCC 
TCCATTCAGC TCTGCGTGCA GAAGCTGCGC ATCCTCATCG AGGACTCGGA CCAGAACTTG 
AAATATCTGG GACTGTTGGC CATGTCGAAA ATCCTAAAGA CGCACCCGAA GAGTGTGCAG 
GCGCACAAGG ATCTCATACT CGCCTGCCTG GACGACAAGG ACGAATCGAT ACGTCTGCGC 
GCCCTGGACC TGCTCTACGG CATGGTCTCG AAGAAGAACC TTATGGAGAT TGTTAAGCGA 
TTGCTGGGCC ACATGGAGCG GGCAGAGGGT TCGGCCTATC GGGACGAATT GCTTTACAAG 
GTGATCGAGA TATGCGCGCA GAGTTCGTAT CTGTATGTGA CCAACTTCGA GTGGTATCTA 
ACTGTGCTCG TCGAGCTCAT TCAACTTGAG GCTGGCTCAC GGCACGGTCG CCTTATAGCA 
GAGCAACTAC TTGACGTGGC CATTCGAGTG CCTGTTGTTC GGCAATTTGC CGTCAACGAG 
ATGACCAATC TGCTGGACAC GTTCACCGTT TCGGCGCAAA GTAATTCCAT GTACGAGGTG 
CTCTACGCCG CCGCCTGGAT CGTTGGCGAG TTTGCTGGCG AACTGGAGGA TGCGGAGAAG 
ACGCTCAACA TCCTGCTCCG TCCGAGGCTA TTGCCCGGTC ACATCCAGGG TGTCTATGTG 
CAGAACGTGA TTAAGCTGTT CGCTCGCCTG GCCACCACGT GTTTAGAGCT TCAGGATCTG 
CCTGGCTTGG TGACACTCTG CGACCATGTG TTGGACAAGC TTCAGCACTT CAATGGTTCC 
AGCGACATTG AGGTGCAAGA GCGAGCCAAT TCCGCTTGCA TGCTGATCGA GATGCTGCGC 
AACCAGCTGT CCACGTCGAC CGATGCCATG GCCATGGACA CGACGACGGA GGGCGGCATT 
CCGCCGCTGG CCATTGAGAT TGTCCAGGAG ATGACGCTGC TGTTCACAGG CGAGCTAATA 
CCGGTGGCGC CCAAGGCGCA ACGGAAGGTG CCCCTGCCAG ATGGTCTCGA TCTGGACGAG 
TGGATTAATG CACCGCCACC GGAAGATGCG GCAAGCAGTT CATCCTCGGA GCACGACAAG 
GACGAGCTAT TCGTTAGTGC CACGCAGGCA GGAACGGGAG CTGATGGCGG CGAAAAGCGG 
CGACAGAGTC TCGAGCTCAC GCCTGAGCAA CTGGAGCGGC AGCGAATGGC TCGTCTCATT 
GAGCAGTCGA ACAATCCTCA CTACCTCAAG TCCACGCCCA CGGCGAGTGG TGCCTCGAAT 
GCGGATCAGT ATGACAATAT TGATGACATA CCCATTACGG AGCTGCCACT GGACATGGAG 
GGCGTGGCCG CACTGCGCGT GGGCATCACC AAGCGGTCGG ATAAGTACCT GCAGGAACAG 
CAGGCGGCGC AGGGCAGCAA GGATGGCAAG AAGAAGCATA AAAAGGGTAA AAAGAGCAAA 
AAGGCCAAAA ATAAAGTGGC ATACAACAGC AGCTCCGAAT CGGAAGAACC GAAACCCTTG 
CACATTGTGA ACACCACGCT AGACATGCCC GAAGGTGTCT CCATGTCGGA CAGCGAGGAC 
AAGGATGGCA AATACGATCC AAATGATCCG CATCGAGCCC TGGACATTGA ATTGGACATT 
ACGGAGTTCG AGGCGCCCGC GGTGAGGTCA GCGTCCAAAA AGTCAGCGGC GGACAAAGAG 
AATCTCAAGA CGCCGGCGGA TTCGGCCGGC GGTGGTAACG CCGCCAAGAA GGATAGGAAA 
AAGGACAAGG ACAAGGACAA GGAGAGGAAG GTGAAGCGGG AGCATCGGGA GAGCAAGCGG 
GAACGCAAGG AGGCTGCTGT TCAGTCGGTG ATCGATCTCA TTGACGCCGA CACGCCCACT 
CCCAGTCCCA GCCACATCTC CGCCACCAGC AACAACAACA ACACTTCAAC TGTCCTTCCA 
GATGCGCCCA AGCATCACAA AAAGAAGAAG AACAAGGAGA AGACGACGGA TGAAGCGCCT 
GATGCACTGG CCACCGCCAC CGGGAGCAGC ATCATCGATG TGGGCGGCGA GGAGGCATCC 
GAGGTGGCGT CCAAGGTGCA CAAGAAGAAG CATAAAAAGG AGAAGTCACA GCGCAAGGAG 
AAGAAGAAGG CTTCAGAATC TGCATCCGTA TCCGCCATCG TTTCGATTGG CGACTACGAA 
CAGCCACTGG GAATCTCAAC GCCCAGCAAA GAGATTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259528.1
CDS	218..3319
Translation	MALKKVKGNFFERMFDKNLTDLVRGIRNNKDNEAKYISTCIEEIKQELRQDNISVKCNAVAKLTYIQMLGYDISWAGFNIIEVMSSSRFTCKRIGYLAASQCFHPDSELLMLTTNMIRKDLNSQNQYDAGVALSGLSCFISPDLSRDLANDIMTLMSSTKPYLRMKAVLMMYKVFLRYPEALRPAFPKLKEKLEDPDPGVQSAAVNVICELARKNPKNYLPLAPIFFKLMTTSTNNWMLIKIIKLFASLTTIEPALGRKLTQPLIEIISSTSAMSLLYECINTVIAVLISISSGMPNHSASIQLCVQKLRILIEDSDQNLKYLGLLAMSKILKTHPKSVQAHKDLILACLDDKDESIRLRALDLLYGMVSKKNLMEIVKRLLGHMERAEGSAYRDELLYKVIEICAQSSYLYVTNFEWYLTVLVELIQLEAGSRHGRLIAEQLLDVAIRVPVVRQFAVNEMTNLLDTFTVSAQSNSMYEVLYAAAWIVGEFAGELEDAEKTLNILLRPRLLPGHIQGVYVQNVIKLFARLATTCLELQDLPGLVTLCDHVLDKLQHFNGSSDIEVQERANSACMLIEMLRNQLSTSTDAMAMDTTTEGGIPPLAIEIVQEMTLLFTGELIPVAPKAQRKVPLPDGLDLDEWINAPPPEDAASSSSSEHDKDELFVSATQAGTGADGGEKRRQSLELTPEQLERQRMARLIEQSNNPHYLKSTPTASGASNADQYDNIDDIPITELPLDMEGVAALRVGITKRSDKYLQEQQAAQGSKDGKKKHKKGKKSKKAKNKVAYNSSSESEEPKPLHIVNTTLDMPEGVSMSDSEDKDGKYDPNDPHRALDIELDITEFEAPAVRSASKKSAADKENLKTPADSAGGGNAAKKDRKKDKDKDKERKVKREHRESKRERKEAAVQSVIDLIDADTPTPSPSHISATSNNNNTSTVLPDAPKHHKKKKNKEKTTDEAPDALATATGSSIIDVGGEEASEVASKVHKKKHKKEKSQRKEKKKASESASVSAIVSIGDYEQPLGISTPSKEIL

Target ORF information:

RefSeq Version	NM_001272599.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster garnet (g), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272599.1

ORF Insert Sequence:

ATGGCACTGA AAAAAGTGAA GGGTAACTTT TTCGAGCGTA TGTTTGATAA AAACCTCACC 
GATTTGGTGC GCGGCATTAG GAACAACAAG GATAATGAGG CCAAATACAT ATCGACGTGC 
ATTGAGGAGA TCAAACAGGA GCTGCGACAG GACAATATAA GTGTCAAGTG TAATGCAGTG 
GCCAAGTTGA CCTACATACA AATGCTGGGC TACGACATCT CCTGGGCGGG CTTCAATATC 
ATCGAGGTGA TGAGCTCATC CAGATTTACC TGCAAGCGAA TTGGCTATCT GGCGGCTAGC 
CAATGTTTCC ATCCGGACAG CGAGCTGCTC ATGCTGACCA CGAATATGAT ACGCAAGGAT 
CTCAACTCAC AGAATCAATA CGATGCGGGT GTGGCGCTCA GTGGACTGAG CTGCTTTATA 
TCGCCCGATC TGTCCCGCGA CTTGGCCAAT GATATTATGA CACTGATGAG TTCAACAAAA 
CCGTATCTGC GCATGAAAGC CGTCCTTATG ATGTATAAAG TATTTCTACG CTATCCCGAG 
GCATTGAGAC CGGCATTTCC CAAACTCAAA GAGAAACTTG AGGATCCTGA TCCAGGTGTT 
CAATCAGCTG CCGTGAATGT GATTTGTGAA CTAGCACGTA AGAATCCAAA AAACTACCTG 
CCCTTGGCGC CGATCTTCTT CAAGCTAATG ACCACATCGA CCAACAATTG GATGCTGATC 
AAGATCATCA AGCTGTTCGC GAGCCTCACG ACAATTGAAC CGGCGCTGGG ACGCAAATTG 
ACTCAGCCCC TAATTGAAAT TATTTCCAGC ACCTCGGCGA TGAGTCTCCT GTATGAGTGC 
ATCAACACGG TGATCGCGGT GCTCATCAGC ATTAGCAGCG GCATGCCCAA CCACAGTGCC 
TCCATTCAGC TCTGCGTGCA GAAGCTGCGC ATCCTCATCG AGGACTCGGA CCAGAACTTG 
AAATATCTGG GACTGTTGGC CATGTCGAAA ATCCTAAAGA CGCACCCGAA GAGTGTGCAG 
GCGCACAAGG ATCTCATACT CGCCTGCCTG GACGACAAGG ACGAATCGAT ACGTCTGCGC 
GCCCTGGACC TGCTCTACGG CATGGTCTCG AAGAAGAACC TTATGGAGAT TGTTAAGCGA 
TTGCTGGGCC ACATGGAGCG GGCAGAGGGT TCGGCCTATC GGGACGAATT GCTTTACAAG 
GTGATCGAGA TATGCGCGCA GAGTTCGTAT CTGTATGTGA CCAACTTCGA GTGGTATCTA 
ACTGTGCTCG TCGAGCTCAT TCAACTTGAG GCTGGCTCAC GGCACGGTCG CCTTATAGCA 
GAGCAACTAC TTGACGTGGC CATTCGAGTG CCTGTTGTTC GGCAATTTGC CGTCAACGAG 
ATGACCAATC TGCTGGACAC GTTCACCGTT TCGGCGCAAA GTAATTCCAT GTACGAGGTG 
CTCTACGCCG CCGCCTGGAT CGTTGGCGAG TTTGCTGGCG AACTGGAGGA TGCGGAGAAG 
ACGCTCAACA TCCTGCTCCG TCCGAGGCTA TTGCCCGGTC ACATCCAGGG TGTCTATGTG 
CAGAACGTGA TTAAGCTGTT CGCTCGCCTG GCCACCACGT GTTTAGAGCT TCAGGATCTG 
CCTGGCTTGG TGACACTCTG CGACCATGTG TTGGACAAGC TTCAGCACTT CAATGGTTCC 
AGCGACATTG AGGTGCAAGA GCGAGCCAAT TCCGCTTGCA TGCTGATCGA GATGCTGCGC 
AACCAGCTGT CCACGTCGAC CGATGCCATG GCCATGGACA CGACGACGGA GGGCGGCATT 
CCGCCGCTGG CCATTGAGAT TGTCCAGGAG ATGACGCTGC TGTTCACAGG CGAGCTAATA 
CCGGTGGCGC CCAAGGCGCA ACGGAAGGTG CCCCTGCCAG ATGGTCTCGA TCTGGACGAG 
TGGATTAATG CACCGCCACC GGAAGATGCG GCAAGCAGTT CATCCTCGGA GCACGACAAG 
GACGAGCTAT TCGTTAGTGC CACGCAGGCA GGAACGGGAG CTGATGGCGG CGAAAAGCGG 
CGACAGAGTC TCGAGCTCAC GCCTGAGCAA CTGGAGCGGC AGCGAATGGC TCGTCTCATT 
GAGCAGTCGA ACAATCCTCA CTACCTCAAG TCCACGCCCA CGGCGAGTGG TGCCTCGAAT 
GCGGATCAGT ATGACAATAT TGATGACATA CCCATTACGG AGCTGCCACT GGACATGGAG 
GGCGTGGCCG CACTGCGCGT GGGCATCACC AAGCGGTCGG ATAAGTACCT GCAGGAACAG 
CAGGCGGCGC AGGGCAGCAA GGATGGCAAG AAGAAGCATA AAAAGGGTAA AAAGAGCAAA 
AAGGCCAAAA ATAAAGTGGC ATACAACAGC AGCTCCGAAT CGGAAGAACC GAAACCCTTG 
CACATTGTGA ACACCACGCT AGACATGCCC GAAGGTGTCT CCATGTCGGA CAGCGAGGAC 
AAGGATGGCA AATACGATCC AAATGATCCG CATCGAGCCC TGGACATTGA ATTGGACATT 
ACGGAGTTCG AGGCGCCCGC GGTGAGGTCA GCGTCCAAAA AGTCAGCGGC GGACAAAGAG 
AATCTCAAGA CGCCGGCGGA TTCGGCCGGC GGTGGTAACG CCGCCAAGAA GGATAGGAAA 
AAGGACAAGG ACAAGGACAA GGAGAGGAAG GTGAAGCGGG AGCATCGGGA GAGCAAGCGG 
GAACGCAAGG AGGCTGCTGT TCAGTCGGTG ATCGATCTCA TTGACGCCGA CACGCCCACT 
CCCAGTCCCA GCCACATCTC CGCCACCAGC AACAACAACA ACACTTCAAC TGTCCTTCCA 
GATGCGCCCA AGCATCACAA AAAGAAGAAG AACAAGGAGA AGACGACGGA TGAAGCGCCT 
GATGCACTGG CCACCGCCAC CGGGAGCAGC ATCATCGATG TGGGCGGCGA GGAGGCATCC 
GAGGTGGCGT CCAAGGTGCA CAAGAAGAAG CATAAAAAGG AGAAGTCACA GCGCAAGGAG 
AAGAAGAAGG CTTCAGAATC TGCATCCGTA TCCGCCATCG TTTCGATTGG CGACTACGAA 
CAGCCACTGG GAATCTCAAC GCCCAGCAAA GAGATTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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g cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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