Apc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apc
Entrez Gene ID	44642
Full Name	APC-like
Synonyms	APC,APC1,Apc1,CG1451,D-APC,D-APC1,DAPC,Dm APC1,Dmel\CG1451,apc,apc 1,apc1,d-APC,dAPC,dAPC1,dApc,dapc1
General protein information	Preferred Names APC-like Names CG1451 gene product from transcript CG1451-RA Apc-PA Apc-PB Apc-PC CG1451-PA CG1451-PB CG1451-PC adenomatous polyposis coli adenomatous polyposis coli 1 adenomatous polyposis coli protein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98E5-98E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276117.1	NP_001263046.1	APC-like, isoform B
NM_057804.3	NP_477152.1	APC-like, isoform A
NM_001316494.1	NP_001303423.1	APC-like, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Apc2	NP_477430.1
Drosophila melanogaster (fruit fly)	Apc	NP_001263046.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in the adult intestine, Ebd acts independently of Ewg in physiological Wnt signaling, but cooperates with Ewg to induce the hyperactivation of Wnt target gene expression following Apc1 loss. These findings have relevance for human tumorigenesis, as Jerky (JRK/JH8), the human Ebd homolog, promotes Wnt pathway hyperactivation and is overexpressed in colorectal, breast, and ovarian cancers
Title: Intestinal stem cell overproliferation resulting from inactivation of the APC tumor suppressor requires the transcription cofactors Earthbound and Erect wing.

We conclude that even in the unstimulated state, a pool of Axin forms membrane-proximal puncta that are dependent on Apc, and that membrane association regulates both Axin levels and Axin's role in the rapid activation of signaling that follows Wnt exposure.
Title: Dual Roles for Membrane Association of Drosophila Axin in Wnt Signaling.

Data indicate that Ras is not required for Wnt signaling activation after APC loss.
Title: APC loss-induced intestinal tumorigenesis in Drosophila: Roles of Ras in Wnt signaling activation and tumor progression.

Drosophila and vertebrate APCs exhibit highly related activities and mechanisms and directly collaborate with formins such as diaphanous.
Title: Drosophila homologues of adenomatous polyposis coli (APC) and the formin diaphanous collaborate by a conserved mechanism to stimulate actin filament assembly.

Non-autonomous crosstalk between the Jak/Stat and Egfr pathways mediates Apc1-driven intestinal stem cell hyperplasia in the Drosophila adult midgut.
Title: Non-autonomous crosstalk between the Jak/Stat and Egfr pathways mediates Apc1-driven intestinal stem cell hyperplasia in the Drosophila adult midgut.

The following Apc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa41220	NM_001316494.1 Latest version!	Drosophila melanogaster APC-like (Apc), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFa06856	NM_001276117.1 Latest version!	Drosophila melanogaster APC-like (Apc), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa06856	NM_057804.3 Latest version!	Drosophila melanogaster APC-like (Apc), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apc ( NM_001316494.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303423.1 Drosophila melanogaster APC-like (Apc), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa41220
Clone ID Related Accession (Same CDS sequence)	NM_001316494.1
Accession Version	NM_001316494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	APC-like, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGGAGC CAACGCTGAC GCCCGATCTG GAGGAGGGCA TCGCCGAGCT CAGTCTGGAG 
GACGTGGATG TGGACTCGGT GCCAGAGCGT AAGCCGCACT TCCTCGACTA CGACGCAGTG 
CCCCCGCCAG ACTATGAAGA AGGCTTTGGG GCCAGTTCCG GGGAGCAGCT AAAGATGGAC 
AAGAAGTACG AGCGGGATGG CGAAGTGAGC GACTACGAAC TGGCCGCCAG TGGCTATACC 
AAGAAGGAGT TCACCCAGGA CGACAACACG CTGCACTTCA CCCAGAGCGC AGTCGGACTG 
GGATCCGGAG CCGTTGGTAA AAAACCAGCA GCCAAGCATT TTCTGGACGA GAACCCCATT 
CCGCCGGACT ACATGTTGGC CCAGGAGCTG CGGGAGATGA GAGAACATCG CAGCCTGGAC 
AGGAACTTCG AGCGACAGTC GGCGCAGCAG CAGCAGCTGG ATGAATTGCC TCCGAGGAAT 
GGAGGCGGCT CTCCAGCCAG CGCAGGTCGT CCATCGCGCA GCAAGGAACC ATCTTACACG 
CTCTCCCGAT TTCTGGACGG AGATGCACCT GCCCCTGCTC CAAGGCTGCC CAAGGGAGCT 
GCCTGGACGA CCAGCTTCGA TGAGCGATAC ACCTCCTCGG CAGTGGAAGC CACCTTGGGA 
TCCAAGGTGG AGTGTGTTTA CTCACTGCTC TCCATGTTGG GCTCAAATGA TCCTCTCGAA 
ATGGCCAAAA AGTTCCTGGA GCTGTCCGGA AATGCTCAAA GTTGTGCAAC TCTTAGACGC 
TCTGGTTGCA TGCCGCTTCT GGTGCAGATG ATGCATGCAC CGGACAACGA CCAGGAGGTG 
AGGAAGTGTG CTGGACAGGC GCTGCACAAT GTGGTGCACA GCCATCCGGA CGAGAAGGCT 
GGCAGAAGGG AAGCCAAGGT GCTCCGACTG CTTGACCAGA TCGTTGACTA CTGCTCCTTC 
CTGAAGACGC TGCTCCAGAG TGGCGGAGAG GCTATTGCAG ATGACTCCGA CCGTCATCCG 
CTGGCCGCCA TCTCTTCACT GATGAAGGTG AGCTTCGATG AGGAGCACCG ACACGCTATG 
TGCGAGCTGG GGGCATTGCA CGCGATCCCC AATCTGGTTC ATTTGGATCA CGCCGTTCAT 
GGACCCAAAC CGGAGGATCA GTGCTGCAAT TCTCTGCGAA GATACGCTTT GATGGCCCTG 
ACCAATCTGA CCTTTGGGGA TGAGAACAAC AAGGCGCTGC TGTGTGGTCA AAAGCAGTTC 
ATGGAGGCCC TGGTGGCCCA ATTGGACTCG GCCCCGGACG ATCTGCTCCA GGTGACGGCC 
AGCGTGCTGC GCAATCTCTC CTGGCGAGCG GACAGCAACA TGAAGGCAGT GCTGAATGAG 
ATTGGAACAG TGACAGCTCT GGCCCTGGCG GCCATGCGGA ATAGGAGTGA GAACACCCTG 
AAGGCCATAC TCTCGGCCCT GTGGAATCTC TCGGCGCACT GCAGCACCAA CAAGGCGGAG 
TTCTGTGCCG TAGATGGGGC CTTGGCATTC CTAGTAGGAA TGCTAAGTTA CGAAGGTCCC 
AGCAAAACAT TGAAGATCAT TGAGAATGCG GGAGGGATCC TGCGGAACGT GTCCAGCCAC 
ATTGCTGTTT GTGAACCTTA TCGGCAGATC CTGCGCCAGC ACAACTGCCT GGCTATTCTG 
CTGCAGCAAT TGAAGTCCGA AAGCCTTACC GTGGTCAGCA ACTCCTGTGG AACTCTATGG 
AACCTGTCAG CTCGCTCTGC CGAGGATCAG AAGTTCCTGT GGGACAATGG AGCAGTGCCC 
ATGCTCCGTT CGTTGATCCA CTCCAAACAC GCCATGATCT CAGAGGGCAG CTCTTCGGCC 
TTGAAGAACC TTTTGAACTT CCGACCAGCT GTTCAAAATC ATCACCAGCT GGATCCAATT 
GCCCGCTCCA TGGGCCTCAA AGCATTGCCC ACTCTGGAGG CACGAAAAGC CAAGGCATTG 
CAACAGGAGC TGGGTGAGCG TCACACGGCC GAGACGTGCG ATAACTTGGA CACGGGCGGA 
AAACTGGATA AAGAACGAGC CTCCAGTTCA TCCAGACGTC ATCCTGCGCC ACGCTTAACC 
CGATCGGCAA TGCTAACGAA GAGCGAGAGC AGGGATTCGG TGTACTCGGC CAAATCCGAT 
TGCGCTTACG ATCATCTAAT TCGCTCAGCC TCTGCTTCAG ATGCCCATCG AAAGGTGAAG 
CCCAAGATCA CGGACTTCGA TCTGGAAATG GAGCAGGACA CGGAGGCAAC CGAGGAACAA 
CCCATTGACT ACTCGGTGAA GTACAGCGAG AATGCCACCA AGACCTCAAC ATATCAGGAA 
ACGGACCTGG ATCAGCCCAC GGACTTCAGC CTTCGCTACG CTGAGAACCA AATTGAATCC 
GACCTGGACA TATCAGGACC AGCAGGCGGA CAAAAGAGCA CTATCACTCC TCCTGCTGAA 
ACAGTTCCCG AGAAATCCGA GGGCCAGGAA ATATTGTTGA TCCTAGACGA CAGTGTTAAG 
TGCTACCAGA CGGAGGATAC CCCTTATGTT ATATCCAATG CGGCTTCAGT CACAGATTTA 
CGAGTCGCTG CTAAAGCGGA TGCAGAAGCG GAAGTCAAGC CAGAAGTTCG CGAGGTGACT 
TCCAAAGAAG GAGCTCCCAA AAAGCTTCCC AAGCTTTCGC AGTGTGGCTC CGGTTCGTAC 
ACTCCAGAGA AACCCATTAA CTACTGCGAG GAGGGAACTC CTGGGTACTT TAGTCGCTAC 
GATTCACTGA GCAGCTTGGA TGAATCCGGT AAGGCTAACC AGGCAATAGT AGGAACGGAT 
GCGGATATCA AGCCCAAATT AGAGAAGCAG GAGGAACAGG AATCCCAGCC AGCAGAGCAG 
GTCCTGACCA AACCTCCCAC CCAGGCAAAC TCGGCATTGG AAACCCCGTT GATGTTCTCG 
CGACGCAGTT CCATGGACTC CCTAGTCCAC GATCCGGATG TCGATGTGGC CAACTGCGAT 
GACAAGAGCT CGGTGGTTAG TGACTTCAGT CGCTTGGCCA GTGGGGTAAT ATCCCCATCC 
GAGATCCCAG ATTCCCCCAC ACAAAGTATG CCCCAATCTC CACGACGAAA TAGTGTCGCT 
GGTTCGGGGC AGAATGTCGA TTCTCCACCG GTTGTAATCC CGGCCAGCCT ACAACCATTA 
CGCAGCGTTT TCGAGGACGA CCTGAGTAGC TTCAACGTGG AGCATACACC TGCTCAGTTC 
TCCACGGCCA CCAGTTTGAG CAACCTGAGC ATAGTGGACG ATGAAAAGGC ACCGGCCAGT 
GTGGCCGAGG AAGACAACGA AGATGAGCTC CTTTTGGCCA ACTGCATCAA CATGGGTATG 
CAGCGGAAGC CAACTGAGGC TGTGAAATCG ACAGTTGTGA ACTCAGAAGT GGACGTGGCT 
GAGGAGACCA TTCGTAGCTA CTGCACCGAA GATACGCCAG CATTGCTCTC CAAAGTACCA 
AGTAACACTA ACCTTTCGGT TATCTCCATG AGCTCCACCG ATCCCAAGGA TGCCACGGCT 
GGTCAGGCAC AGATGTACGC TCATCAGCTA TCCGACGATG TATCTTCCAA TGCATCGGAC 
TGCGGTGGAG CCTCTGGTCA CCTGCTACAG CAGTGCATCC GGGATGGCAT GAAGAAACCC 
CTCGGTGAAG CCACTTCAGA TCCTATAGCC ATGCTTCGAC GAGGCGGAAA CGAATTGCCT 
GGCTATCTGC CCTCTGCCGA TGAAATGAAC AAGTTTTTGG TGGAGGACAG TCCCTGTAAC 
TTCTCAGTGG TCTCTGGTTT ATCTAATCTC ACCGTGGGAT CCAGTCTGGT TGGGCCAGCC 
GTACAGCTCA AGGAGACCGA ACCATCCAGT GCGGATCAGA ATCCTGAGAT GAAGGCGAAG 
CCTGGCAAAC AGGAGCAAGT CCGAAGACCG CCGCATTGGC AAGATGATTC GCTGAGTTCT 
CTATCCATTG ACTCGGAAGA TGACACCAAC TTACTGAGTC AGGCTATTGC CGCTGGTTGC 
AATAGACCGA AATCGAATCT TGGCTTCAGC TCGAACGGCA AGCGCTCCAG CTCGCTGAGC 
TCCTCGCAGC CAATAGCCAT CAATGCGGCC ACCTCGGCCA GCTCCCTGAA TTCGGCGATG 
ACAGTACGCA AGAGCCAACA GCAGGAGTCC TACAGTTCCG TGGACTCTAG CGATTCTAAC 
GACAACCAGT CAAAGTCGCT CTTCGAGCTG TGCATCCTCA AGGGCATGTA CAAGACGAAG 
GAACCCGGCG CCCGGGCCCA GCAGATGCAG GAGCAACCTA TAGTGGGTTC ATCCTCCGTC 
CAGTCGAATC CCAGTCTAAA GCAGTTCGAT TCGCTGCCGG TGCAGCTGCC ATCTAGTGGA 
CAGGTCAAGC GGCAGCGCCA TCATCATCAT CACCATCATC ATAGGGAGCG AGAGCGAGAA 
CGCAAGGACG AGAAGCTGCT GCAGGAGTGC ATCAATACGG GCATCTCGAA GAAGATCAAC 
GCCGTGCCAA AAAAACGTCC TGGCTACGTC TGCGGCTGCA TTGGAACCGT GTCACCCAAT 
GGCAGCTACT ACATCAGCAA GTGCCCTCAG CACAGCAGCT CCAGACGTAG AGCAGAAAGC 
GCACGCCACC AGCAATCCGC AGCAGCAGTC GTCCACGCAC CCGAGCAGCC ACATCCTCCC 
GAATCCTATC GACGCCATCG CCACCGTCAC CGACACAGTA AGAAGTCCAG CAGCTCCAAA 
TCAAGGGAAC GGGAACGCGA GTCAGAACGG TCTCGAGACC GCTACTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303423.1
CDS	join(254..4767,4767..5046)
Translation	MLEPTLTPDLEEGIAELSLEDVDVDSVPERKPHFLDYDAVPPPDYEEGFGASSGEQLKMDKKYERDGEVSDYELAASGYTKKEFTQDDNTLHFTQSAVGLGSGAVGKKPAAKHFLDENPIPPDYMLAQELREMREHRSLDRNFERQSAQQQQLDELPPRNGGGSPASAGRPSRSKEPSYTLSRFLDGDAPAPAPRLPKGAAWTTSFDERYTSSAVEATLGSKVECVYSLLSMLGSNDPLEMAKKFLELSGNAQSCATLRRSGCMPLLVQMMHAPDNDQEVRKCAGQALHNVVHSHPDEKAGRREAKVLRLLDQIVDYCSFLKTLLQSGGEAIADDSDRHPLAAISSLMKVSFDEEHRHAMCELGALHAIPNLVHLDHAVHGPKPEDQCCNSLRRYALMALTNLTFGDENNKALLCGQKQFMEALVAQLDSAPDDLLQVTASVLRNLSWRADSNMKAVLNEIGTVTALALAAMRNRSENTLKAILSALWNLSAHCSTNKAEFCAVDGALAFLVGMLSYEGPSKTLKIIENAGGILRNVSSHIAVCEPYRQILRQHNCLAILLQQLKSESLTVVSNSCGTLWNLSARSAEDQKFLWDNGAVPMLRSLIHSKHAMISEGSSSALKNLLNFRPAVQNHHQLDPIARSMGLKALPTLEARKAKALQQELGERHTAETCDNLDTGGKLDKERASSSSRRHPAPRLTRSAMLTKSESRDSVYSAKSDCAYDHLIRSASASDAHRKVKPKITDFDLEMEQDTEATEEQPIDYSVKYSENATKTSTYQETDLDQPTDFSLRYAENQIESDLDISGPAGGQKSTITPPAETVPEKSEGQEILLILDDSVKCYQTEDTPYVISNAASVTDLRVAAKADAEAEVKPEVREVTSKEGAPKKLPKLSQCGSGSYTPEKPINYCEEGTPGYFSRYDSLSSLDESGKANQAIVGTDADIKPKLEKQEEQESQPAEQVLTKPPTQANSALETPLMFSRRSSMDSLVHDPDVDVANCDDKSSVVSDFSRLASGVISPSEIPDSPTQSMPQSPRRNSVAGSGQNVDSPPVVIPASLQPLRSVFEDDLSSFNVEHTPAQFSTATSLSNLSIVDDEKAPASVAEEDNEDELLLANCINMGMQRKPTEAVKSTVVNSEVDVAEETIRSYCTEDTPALLSKVPSNTNLSVISMSSTDPKDATAGQAQMYAHQLSDDVSSNASDCGGASGHLLQQCIRDGMKKPLGEATSDPIAMLRRGGNELPGYLPSADEMNKFLVEDSPCNFSVVSGLSNLTVGSSLVGPAVQLKETEPSSADQNPEMKAKPGKQEQVRRPPHWQDDSLSSLSIDSEDDTNLLSQAIAAGCNRPKSNLGFSSNGKRSSSLSSSQPIAINAATSASSLNSAMTVRKSQQQESYSSVDSSDSNDNQSKSLFELCILKGMYKTKEPGARAQQMQEQPIVGSSSVQSNPSLKQFDSLPVQLPSSGQVKRQRHHHHHHHHRERERERKDEKLLQECINTGISKKINAVPKKRPGYVCGCIGTVSPNGSYYISKCPQHSSSRRRAESARHQQSAAAVVHAPEQPHPPESYRRHRHRHRHSKKSSSSKSRERERESERSRDRYWV

Target ORF information:

RefSeq Version	NM_001316494.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster APC-like (Apc), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316494.1

ORF Insert Sequence:

ATGTTGGAGC CAACGCTGAC GCCCGATCTG GAGGAGGGCA TCGCCGAGCT CAGTCTGGAG 
GACGTGGATG TGGACTCGGT GCCAGAGCGT AAGCCGCACT TCCTCGACTA CGACGCAGTG 
CCCCCGCCAG ACTATGAAGA AGGCTTTGGG GCCAGTTCCG GGGAGCAGCT AAAGATGGAC 
AAGAAGTACG AGCGGGATGG CGAAGTGAGC GACTACGAAC TGGCCGCCAG TGGCTATACC 
AAGAAGGAGT TCACCCAGGA CGACAACACG CTGCACTTCA CCCAGAGCGC AGTCGGACTG 
GGATCCGGAG CCGTTGGTAA AAAACCAGCA GCCAAGCATT TTCTGGACGA GAACCCCATT 
CCGCCGGACT ACATGTTGGC CCAGGAGCTG CGGGAGATGA GAGAACATCG CAGCCTGGAC 
AGGAACTTCG AGCGACAGTC GGCGCAGCAG CAGCAGCTGG ATGAATTGCC TCCGAGGAAT 
GGAGGCGGCT CTCCAGCCAG CGCAGGTCGT CCATCGCGCA GCAAGGAACC ATCTTACACG 
CTCTCCCGAT TTCTGGACGG AGATGCACCT GCCCCTGCTC CAAGGCTGCC CAAGGGAGCT 
GCCTGGACGA CCAGCTTCGA TGAGCGATAC ACCTCCTCGG CAGTGGAAGC CACCTTGGGA 
TCCAAGGTGG AGTGTGTTTA CTCACTGCTC TCCATGTTGG GCTCAAATGA TCCTCTCGAA 
ATGGCCAAAA AGTTCCTGGA GCTGTCCGGA AATGCTCAAA GTTGTGCAAC TCTTAGACGC 
TCTGGTTGCA TGCCGCTTCT GGTGCAGATG ATGCATGCAC CGGACAACGA CCAGGAGGTG 
AGGAAGTGTG CTGGACAGGC GCTGCACAAT GTGGTGCACA GCCATCCGGA CGAGAAGGCT 
GGCAGAAGGG AAGCCAAGGT GCTCCGACTG CTTGACCAGA TCGTTGACTA CTGCTCCTTC 
CTGAAGACGC TGCTCCAGAG TGGCGGAGAG GCTATTGCAG ATGACTCCGA CCGTCATCCG 
CTGGCCGCCA TCTCTTCACT GATGAAGGTG AGCTTCGATG AGGAGCACCG ACACGCTATG 
TGCGAGCTGG GGGCATTGCA CGCGATCCCC AATCTGGTTC ATTTGGATCA CGCCGTTCAT 
GGACCCAAAC CGGAGGATCA GTGCTGCAAT TCTCTGCGAA GATACGCTTT GATGGCCCTG 
ACCAATCTGA CCTTTGGGGA TGAGAACAAC AAGGCGCTGC TGTGTGGTCA AAAGCAGTTC 
ATGGAGGCCC TGGTGGCCCA ATTGGACTCG GCCCCGGACG ATCTGCTCCA GGTGACGGCC 
AGCGTGCTGC GCAATCTCTC CTGGCGAGCG GACAGCAACA TGAAGGCAGT GCTGAATGAG 
ATTGGAACAG TGACAGCTCT GGCCCTGGCG GCCATGCGGA ATAGGAGTGA GAACACCCTG 
AAGGCCATAC TCTCGGCCCT GTGGAATCTC TCGGCGCACT GCAGCACCAA CAAGGCGGAG 
TTCTGTGCCG TAGATGGGGC CTTGGCATTC CTAGTAGGAA TGCTAAGTTA CGAAGGTCCC 
AGCAAAACAT TGAAGATCAT TGAGAATGCG GGAGGGATCC TGCGGAACGT GTCCAGCCAC 
ATTGCTGTTT GTGAACCTTA TCGGCAGATC CTGCGCCAGC ACAACTGCCT GGCTATTCTG 
CTGCAGCAAT TGAAGTCCGA AAGCCTTACC GTGGTCAGCA ACTCCTGTGG AACTCTATGG 
AACCTGTCAG CTCGCTCTGC CGAGGATCAG AAGTTCCTGT GGGACAATGG AGCAGTGCCC 
ATGCTCCGTT CGTTGATCCA CTCCAAACAC GCCATGATCT CAGAGGGCAG CTCTTCGGCC 
TTGAAGAACC TTTTGAACTT CCGACCAGCT GTTCAAAATC ATCACCAGCT GGATCCAATT 
GCCCGCTCCA TGGGCCTCAA AGCATTGCCC ACTCTGGAGG CACGAAAAGC CAAGGCATTG 
CAACAGGAGC TGGGTGAGCG TCACACGGCC GAGACGTGCG ATAACTTGGA CACGGGCGGA 
AAACTGGATA AAGAACGAGC CTCCAGTTCA TCCAGACGTC ATCCTGCGCC ACGCTTAACC 
CGATCGGCAA TGCTAACGAA GAGCGAGAGC AGGGATTCGG TGTACTCGGC CAAATCCGAT 
TGCGCTTACG ATCATCTAAT TCGCTCAGCC TCTGCTTCAG ATGCCCATCG AAAGGTGAAG 
CCCAAGATCA CGGACTTCGA TCTGGAAATG GAGCAGGACA CGGAGGCAAC CGAGGAACAA 
CCCATTGACT ACTCGGTGAA GTACAGCGAG AATGCCACCA AGACCTCAAC ATATCAGGAA 
ACGGACCTGG ATCAGCCCAC GGACTTCAGC CTTCGCTACG CTGAGAACCA AATTGAATCC 
GACCTGGACA TATCAGGACC AGCAGGCGGA CAAAAGAGCA CTATCACTCC TCCTGCTGAA 
ACAGTTCCCG AGAAATCCGA GGGCCAGGAA ATATTGTTGA TCCTAGACGA CAGTGTTAAG 
TGCTACCAGA CGGAGGATAC CCCTTATGTT ATATCCAATG CGGCTTCAGT CACAGATTTA 
CGAGTCGCTG CTAAAGCGGA TGCAGAAGCG GAAGTCAAGC CAGAAGTTCG CGAGGTGACT 
TCCAAAGAAG GAGCTCCCAA AAAGCTTCCC AAGCTTTCGC AGTGTGGCTC CGGTTCGTAC 
ACTCCAGAGA AACCCATTAA CTACTGCGAG GAGGGAACTC CTGGGTACTT TAGTCGCTAC 
GATTCACTGA GCAGCTTGGA TGAATCCGGT AAGGCTAACC AGGCAATAGT AGGAACGGAT 
GCGGATATCA AGCCCAAATT AGAGAAGCAG GAGGAACAGG AATCCCAGCC AGCAGAGCAG 
GTCCTGACCA AACCTCCCAC CCAGGCAAAC TCGGCATTGG AAACCCCGTT GATGTTCTCG 
CGACGCAGTT CCATGGACTC CCTAGTCCAC GATCCGGATG TCGATGTGGC CAACTGCGAT 
GACAAGAGCT CGGTGGTTAG TGACTTCAGT CGCTTGGCCA GTGGGGTAAT ATCCCCATCC 
GAGATCCCAG ATTCCCCCAC ACAAAGTATG CCCCAATCTC CACGACGAAA TAGTGTCGCT 
GGTTCGGGGC AGAATGTCGA TTCTCCACCG GTTGTAATCC CGGCCAGCCT ACAACCATTA 
CGCAGCGTTT TCGAGGACGA CCTGAGTAGC TTCAACGTGG AGCATACACC TGCTCAGTTC 
TCCACGGCCA CCAGTTTGAG CAACCTGAGC ATAGTGGACG ATGAAAAGGC ACCGGCCAGT 
GTGGCCGAGG AAGACAACGA AGATGAGCTC CTTTTGGCCA ACTGCATCAA CATGGGTATG 
CAGCGGAAGC CAACTGAGGC TGTGAAATCG ACAGTTGTGA ACTCAGAAGT GGACGTGGCT 
GAGGAGACCA TTCGTAGCTA CTGCACCGAA GATACGCCAG CATTGCTCTC CAAAGTACCA 
AGTAACACTA ACCTTTCGGT TATCTCCATG AGCTCCACCG ATCCCAAGGA TGCCACGGCT 
GGTCAGGCAC AGATGTACGC TCATCAGCTA TCCGACGATG TATCTTCCAA TGCATCGGAC 
TGCGGTGGAG CCTCTGGTCA CCTGCTACAG CAGTGCATCC GGGATGGCAT GAAGAAACCC 
CTCGGTGAAG CCACTTCAGA TCCTATAGCC ATGCTTCGAC GAGGCGGAAA CGAATTGCCT 
GGCTATCTGC CCTCTGCCGA TGAAATGAAC AAGTTTTTGG TGGAGGACAG TCCCTGTAAC 
TTCTCAGTGG TCTCTGGTTT ATCTAATCTC ACCGTGGGAT CCAGTCTGGT TGGGCCAGCC 
GTACAGCTCA AGGAGACCGA ACCATCCAGT GCGGATCAGA ATCCTGAGAT GAAGGCGAAG 
CCTGGCAAAC AGGAGCAAGT CCGAAGACCG CCGCATTGGC AAGATGATTC GCTGAGTTCT 
CTATCCATTG ACTCGGAAGA TGACACCAAC TTACTGAGTC AGGCTATTGC CGCTGGTTGC 
AATAGACCGA AATCGAATCT TGGCTTCAGC TCGAACGGCA AGCGCTCCAG CTCGCTGAGC 
TCCTCGCAGC CAATAGCCAT CAATGCGGCC ACCTCGGCCA GCTCCCTGAA TTCGGCGATG 
ACAGTACGCA AGAGCCAACA GCAGGAGTCC TACAGTTCCG TGGACTCTAG CGATTCTAAC 
GACAACCAGT CAAAGTCGCT CTTCGAGCTG TGCATCCTCA AGGGCATGTA CAAGACGAAG 
GAACCCGGCG CCCGGGCCCA GCAGATGCAG GAGCAACCTA TAGTGGGTTC ATCCTCCGTC 
CAGTCGAATC CCAGTCTAAA GCAGTTCGAT TCGCTGCCGG TGCAGCTGCC ATCTAGTGGA 
CAGGTCAAGC GGCAGCGCCA TCATCATCAT CACCATCATC ATAGGGAGCG AGAGCGAGAA 
CGCAAGGACG AGAAGCTGCT GCAGGAGTGC ATCAATACGG GCATCTCGAA GAAGATCAAC 
GCCGTGCCAA AAAAACGTCC TGGCTACGTC TGCGGCTGCA TTGGAACCGT GTCACCCAAT 
GGCAGCTACT ACATCAGCAA GTGCCCTCAG CACAGCAGCT CCAGACGTAG AGCAGAAAGC 
GCACGCCACC AGCAATCCGC AGCAGCAGTC GTCCACGCAC CCGAGCAGCC ACATCCTCCC 
GAATCCTATC GACGCCATCG CCACCGTCAC CGACACAGTA AGAAGTCCAG CAGCTCCAAA 
TCAAGGGAAC GGGAACGCGA GTCAGAACGG TCTCGAGACC GCTACTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( NM_001276117.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263046.1 Drosophila melanogaster APC-like (Apc), transcript variant B, mRNA.

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CloneID	OFa06856
Clone ID Related Accession (Same CDS sequence)	NM_001276117.1 , NM_057804.3
Accession Version	NM_001276117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	APC-like, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGGAGC CAACGCTGAC GCCCGATCTG GAGGAGGGCA TCGCCGAGCT CAGTCTGGAG 
GACGTGGATG TGGACTCGGT GCCAGAGCGT AAGCCGCACT TCCTCGACTA CGACGCAGTG 
CCCCCGCCAG ACTATGAAGA AGGCTTTGGG GCCAGTTCCG GGGAGCAGCT AAAGATGGAC 
AAGAAGTACG AGCGGGATGG CGAAGTGAGC GACTACGAAC TGGCCGCCAG TGGCTATACC 
AAGAAGGAGT TCACCCAGGA CGACAACACG CTGCACTTCA CCCAGAGCGC AGTCGGACTG 
GGATCCGGAG CCGTTGGTAA AAAACCAGCA GCCAAGCATT TTCTGGACGA GAACCCCATT 
CCGCCGGACT ACATGTTGGC CCAGGAGCTG CGGGAGATGA GAGAACATCG CAGCCTGGAC 
AGGAACTTCG AGCGACAGTC GGCGCAGCAG CAGCAGCTGG ATGAATTGCC TCCGAGGAAT 
GGAGGCGGCT CTCCAGCCAG CGCAGGTCGT CCATCGCGCA GCAAGGAACC ATCTTACACG 
CTCTCCCGAT TTCTGGACGG AGATGCACCT GCCCCTGCTC CAAGGCTGCC CAAGGGAGCT 
GCCTGGACGA CCAGCTTCGA TGAGCGATAC ACCTCCTCGG CAGTGGAAGC CACCTTGGGA 
TCCAAGGTGG AGTGTGTTTA CTCACTGCTC TCCATGTTGG GCTCAAATGA TCCTCTCGAA 
ATGGCCAAAA AGTTCCTGGA GCTGTCCGGA AATGCTCAAA GTTGTGCAAC TCTTAGACGC 
TCTGGTTGCA TGCCGCTTCT GGTGCAGATG ATGCATGCAC CGGACAACGA CCAGGAGGTG 
AGGAAGTGTG CTGGACAGGC GCTGCACAAT GTGGTGCACA GCCATCCGGA CGAGAAGGCT 
GGCAGAAGGG AAGCCAAGGT GCTCCGACTG CTTGACCAGA TCGTTGACTA CTGCTCCTTC 
CTGAAGACGC TGCTCCAGAG TGGCGGAGAG GCTATTGCAG ATGACTCCGA CCGTCATCCG 
CTGGCCGCCA TCTCTTCACT GATGAAGGTG AGCTTCGATG AGGAGCACCG ACACGCTATG 
TGCGAGCTGG GGGCATTGCA CGCGATCCCC AATCTGGTTC ATTTGGATCA CGCCGTTCAT 
GGACCCAAAC CGGAGGATCA GTGCTGCAAT TCTCTGCGAA GATACGCTTT GATGGCCCTG 
ACCAATCTGA CCTTTGGGGA TGAGAACAAC AAGGCGCTGC TGTGTGGTCA AAAGCAGTTC 
ATGGAGGCCC TGGTGGCCCA ATTGGACTCG GCCCCGGACG ATCTGCTCCA GGTGACGGCC 
AGCGTGCTGC GCAATCTCTC CTGGCGAGCG GACAGCAACA TGAAGGCAGT GCTGAATGAG 
ATTGGAACAG TGACAGCTCT GGCCCTGGCG GCCATGCGGA ATAGGAGTGA GAACACCCTG 
AAGGCCATAC TCTCGGCCCT GTGGAATCTC TCGGCGCACT GCAGCACCAA CAAGGCGGAG 
TTCTGTGCCG TAGATGGGGC CTTGGCATTC CTAGTAGGAA TGCTAAGTTA CGAAGGTCCC 
AGCAAAACAT TGAAGATCAT TGAGAATGCG GGAGGGATCC TGCGGAACGT GTCCAGCCAC 
ATTGCTGTTT GTGAACCTTA TCGGCAGATC CTGCGCCAGC ACAACTGCCT GGCTATTCTG 
CTGCAGCAAT TGAAGTCCGA AAGCCTTACC GTGGTCAGCA ACTCCTGTGG AACTCTATGG 
AACCTGTCAG CTCGCTCTGC CGAGGATCAG AAGTTCCTGT GGGACAATGG AGCAGTGCCC 
ATGCTCCGTT CGTTGATCCA CTCCAAACAC GCCATGATCT CAGAGGGCAG CTCTTCGGCC 
TTGAAGAACC TTTTGAACTT CCGACCAGCT GTTCAAAATC ATCACCAGCT GGATCCAATT 
GCCCGCTCCA TGGGCCTCAA AGCATTGCCC ACTCTGGAGG CACGAAAAGC CAAGGCATTG 
CAACAGGAGC TGGGTGAGCG TCACACGGCC GAGACGTGCG ATAACTTGGA CACGGGCGGA 
AAACTGGATA AAGAACGAGC CTCCAGTTCA TCCAGACGTC ATCCTGCGCC ACGCTTAACC 
CGATCGGCAA TGCTAACGAA GAGCGAGAGC AGGGATTCGG TGTACTCGGC CAAATCCGAT 
TGCGCTTACG ATCATCTAAT TCGCTCAGCC TCTGCTTCAG ATGCCCATCG AAAGGTGAAG 
CCCAAGATCA CGGACTTCGA TCTGGAAATG GAGCAGGACA CGGAGGCAAC CGAGGAACAA 
CCCATTGACT ACTCGGTGAA GTACAGCGAG AATGCCACCA AGACCTCAAC ATATCAGGAA 
ACGGACCTGG ATCAGCCCAC GGACTTCAGC CTTCGCTACG CTGAGAACCA AATTGAATCC 
GACCTGGACA TATCAGGACC AGCAGGCGGA CAAAAGAGCA CTATCACTCC TCCTGCTGAA 
ACAGTTCCCG AGAAATCCGA GGGCCAGGAA ATATTGTTGA TCCTAGACGA CAGTGTTAAG 
TGCTACCAGA CGGAGGATAC CCCTTATGTT ATATCCAATG CGGCTTCAGT CACAGATTTA 
CGAGTCGCTG CTAAAGCGGA TGCAGAAGCG GAAGTCAAGC CAGAAGTTCG CGAGGTGACT 
TCCAAAGAAG GAGCTCCCAA AAAGCTTCCC AAGCTTTCGC AGTGTGGCTC CGGTTCGTAC 
ACTCCAGAGA AACCCATTAA CTACTGCGAG GAGGGAACTC CTGGGTACTT TAGTCGCTAC 
GATTCACTGA GCAGCTTGGA TGAATCCGGT AAGGCTAACC AGGCAATAGT AGGAACGGAT 
GCGGATATCA AGCCCAAATT AGAGAAGCAG GAGGAACAGG AATCCCAGCC AGCAGAGCAG 
GTCCTGACCA AACCTCCCAC CCAGGCAAAC TCGGCATTGG AAACCCCGTT GATGTTCTCG 
CGACGCAGTT CCATGGACTC CCTAGTCCAC GATCCGGATG TCGATGTGGC CAACTGCGAT 
GACAAGAGCT CGGTGGTTAG TGACTTCAGT CGCTTGGCCA GTGGGGTAAT ATCCCCATCC 
GAGATCCCAG ATTCCCCCAC ACAAAGTATG CCCCAATCTC CACGACGAAA TAGTGTCGCT 
GGTTCGGGGC AGAATGTCGA TTCTCCACCG GTTGTAATCC CGGCCAGCCT ACAACCATTA 
CGCAGCGTTT TCGAGGACGA CCTGAGTAGC TTCAACGTGG AGCATACACC TGCTCAGTTC 
TCCACGGCCA CCAGTTTGAG CAACCTGAGC ATAGTGGACG ATGAAAAGGC ACCGGCCAGT 
GTGGCCGAGG AAGACAACGA AGATGAGCTC CTTTTGGCCA ACTGCATCAA CATGGGTATG 
CAGCGGAAGC CAACTGAGGC TGTGAAATCG ACAGTTGTGA ACTCAGAAGT GGACGTGGCT 
GAGGAGACCA TTCGTAGCTA CTGCACCGAA GATACGCCAG CATTGCTCTC CAAAGTACCA 
AGTAACACTA ACCTTTCGGT TATCTCCATG AGCTCCACCG ATCCCAAGGA TGCCACGGCT 
GGTCAGGCAC AGATGTACGC TCATCAGCTA TCCGACGATG TATCTTCCAA TGCATCGGAC 
TGCGGTGGAG CCTCTGGTCA CCTGCTACAG CAGTGCATCC GGGATGGCAT GAAGAAACCC 
CTCGGTGAAG CCACTTCAGA TCCTATAGCC ATGCTTCGAC GAGGCGGAAA CGAATTGCCT 
GGCTATCTGC CCTCTGCCGA TGAAATGAAC AAGTTTTTGG TGGAGGACAG TCCCTGTAAC 
TTCTCAGTGG TCTCTGGTTT ATCTAATCTC ACCGTGGGAT CCAGTCTGGT TGGGCCAGCC 
GTACAGCTCA AGGAGACCGA ACCATCCAGT GCGGATCAGA ATCCTGAGAT GAAGGCGAAG 
CCTGGCAAAC AGGAGCAAGT CCGAAGACCG CCGCATTGGC AAGATGATTC GCTGAGTTCT 
CTATCCATTG ACTCGGAAGA TGACACCAAC TTACTGAGTC AGGCTATTGC CGCTGGTTGC 
AATAGACCGA AATCGAATCT TGGCTTCAGC TCGAACGGCA AGCGCTCCAG CTCGCTGAGC 
TCCTCGCAGC CAATAGCCAT CAATGCGGCC ACCTCGGCCA GCTCCCTGAA TTCGGCGATG 
ACAGTACGCA AGAGCCAACA GCAGGAGTCC TACAGTTCCG TGGACTCTAG CGATTCTAAC 
GACAACCAGT CAAAGTCGCT CTTCGAGCTG TGCATCCTCA AGGGCATGTA CAAGACGAAG 
GAACCCGGCG CCCGGGCCCA GCAGATGCAG GAGCAACCTA TAGTGGGTTC ATCCTCCGTC 
CAGTCGAATC CCAGTCTAAA GCAGTTCGAT TCGCTGCCGG TGCAGCTGCC ATCTAGTGGA 
CAGGTCAAGC GGCAGCGCCA TCATCATCAT CACCATCATC ATAGGGAGCG AGAGCGAGAA 
CGCAAGGACG AGAAGCTGCT GCAGGAGTGC ATCAATACGG GCATCTCGAA GAAGATCAAC 
GCCGTGCCAA AAAACGTCCT GGCTACGTCT GCGGCTGCAT TGGAACCGTG TCACCCAATG 
GCAGCTACTA CATCAGCAAG TGCCCTCAGC ACAGCAGCTC CAGACGTAGA GCAGAAAGCG 
CACGCCACCA GCAATCCGCA GCAGCAGTCG TCCACGCACC CGAGCAGCCA CATCCTCCCG 
AATCCTATCG ACGCCATCGC CACCGTCACC GACACAGTAA GAAGTCCAGC AGCTCCAAAT 
CAAGGGAACG GGAACGCGAG TCAGAACGGT CTCGAGACCG CTACTGGGTC TAAGGACTTG 
GATTCGGAAG ATCGATCCAG CGATGAATCA AATCAGTCCT TCATCATGGA AACCATGGTC 
AGGTTGGACA GTGCTCTCAA CGAAACGTGC ATCTCCGGAG CAAGCGAGAA GCACAAGGAT 
CCCGACCTGA TGCTGAAGTC TGTGGAGAGA CTGACCATGG AGTTTGTTAC GTCGGCGGAG 
CAGTTGCGCA GCAGCAGCCA CAACCACAGC AGCAGCAACA GCCACAAGAA CAACAGTAGC 
AACAACACCT GGAACGAGAG CACCTGTCCC AATGATGTGA GTTTCCCCAG CGTCAGTCAA 
ACGGCACCGG TTCTGGCCTC CTTGAGCTTG GATGAAGATG CCACGGAGGC GAGATCCCTG 
CACGAGTTGA TTGAGATAAC GCCCACGAAT GAACAGCAAC CGGAATCGCT CGAAGGGGAA 
ACTGATACCC TGGTTAATGG TCATGCTGAT AGCTACTCAG GATCGTCTGG AGGTCTTAAC 
TTCCAGTTGG GTGGGCAGGT GCAGAATGCT GGCGTTAGAT TGGAGCCGCA AAGACTATTA 
TTTAATGGAA CTAGTGCCTC CATTATGACA AACTCGACCA TGATTGCGTT CGAAGCGCGA 
GCTCTGGCGG AAAATCTACT CCAGCCCGCT GCCACTGACG ATGACACCAC GGAGATGACC 
TTCAGCTTGA ACAGTTTGGA TCTGGATAAT ATTCGTCCGC CGTCGGGAAT GGAATCGCTG 
AACAGCTGCT ACCAGGATCA CTCCCAGCCT AGTTCTCTGC GCCAGGCGAT GCCCAGCAAG 
AGTCCACGCT TCGCGCGAAA AATGTTTCCA GCCAATTTGG TAGCCAGACG AGCACTGGGT 
CACTTAGCTG GCAGTGCGGA GAGCGTGAAC TCCAGCTGCA ACCTGCTGGA CAACATCAAG 
CCTCCGTCAC TGATGGATGA GCTGCTGGAC TCGATGATCA GTGTGGACAG CATTCAATCT 
GAGGTGGCCG ATGGCGAGCA GGACTGCAGC ATGGCCACCA CCATTTCCGT GTCCAACTAT 
GAGACGGCTG CTTGCGATGA TCAGACGATG ACCGTGCTCC AAAGTTGTTT CGACGAGGAT 
GAGGATGCCA CAATGAATGA CTACAGCTCG GCCGAGTCCA CACCCAAGCA CGGATCTACT 
CCATCACCCA ATCGTCGTTC CCTCACACCG AAAGACAAGC GACGTCTCAC AAAGGATCGC 
TTCAAGACCT ACACCATAGC CACCAGCTGC GAGATGGAAG CACCAGAGGC CAATGAAACT 
TTGCAGATAG AAATTGTGGA AGCTGCCGTT CCTGTGGCCA CGCCATCGCC ACGCGCCAAT 
GGAAGGAGAA GGGGCAGTGC GGAGCGGTAC AAGACCCAAC TGATTGAATG TCCCCTGGCT 
TTGATACAAC CCCAACCCGA TGATTGCCCA AGCGAACAGC TCTCCTCAAT TCGAGCAATG 
ATGCAACAGT TCACTTTCAT CACGGACATT AACATTGGAC ACAGTCAGGA GACTTGCGAG 
AGTACTGATC ATCCAGAAGA CGCAGGGGAA TCCCCGGAGT GCGATCAGAA CTCTGAGACG 
GAGTCGTGCG ATGGTCAGGA ACCGGATCAG CTGCCTCCTC CTCCGTCAAT TGTGGACCTT 
AGAACATCAG TCGTTAAGCC CACAACCTTG GAGCCCGCCA CGGCCGTAAA ACTGGTGCGA 
GGTCGCAAGA AACCAGCCTA TGTCTCACCC TACAGCATGC AAAGTCAACG GAATAGCAAC 
AATGCCGCCC CGTCCAAGAA GAAAACCCTA TCTCCAACAA TTGCCAAACG AAGTCTGGTG 
CCCGGCGGTT CAGGAGTTAG GTTACCAGCG AAAAAGAAGC CGACGCCGCC GCCTGAGCCG 
GCACCCGCAC GCTTGGAACG CCAGGGAACC TTTGTCAAGG ACGAGCCCAC TAACTCCAAT 
GTTCAAGTGC CCGTGGTGGA GACAAAACCG GCTCAAACCA GTCCCACTCA CCGCGCCTCG 
AAGTTACCCA CAAAGAAAGG CACGGCCAGC GGTGGGTCAC CATCGAAAGC GGGCTCTCCC 
AAGAGGATTC CCTTGGCGCC GGCCCGCCGA ATGACGCCTC AAAGGGCAAA CACCAGTCTG 
CGACTAGCTG CGGGCAAATC TCACGCTGCC TCACGTGTGG TCTCCGGCCG CGTGTCGTCC 
ACTACTCCAC CATCCCGCAG CAACTCCAAC CTAAATGGAA GCAGTGCAGC CGCAGCAGCT 
GCGGCCAAGA TCAACCATGC GCAAAGTCGT ATCGCCAACA TCTGGAAGAG AGTGGACGAG 
GCCAAAACCA AGCAATCGTC ATCCAACCTG AGGACGCAGA AAACCAAATC TTCCAACATG 
CTCAACGCAA ATGGAACCAA GCCGACGCTC CTGCGCAGCT CCACGTTTGA CAATACCCCC 
AGCACCGCTG GAGGAGTCAA GTCCAAGCTG CCAGTGGTGG GCGCGCGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263046.1
CDS	130..7383
Translation	MLEPTLTPDLEEGIAELSLEDVDVDSVPERKPHFLDYDAVPPPDYEEGFGASSGEQLKMDKKYERDGEVSDYELAASGYTKKEFTQDDNTLHFTQSAVGLGSGAVGKKPAAKHFLDENPIPPDYMLAQELREMREHRSLDRNFERQSAQQQQLDELPPRNGGGSPASAGRPSRSKEPSYTLSRFLDGDAPAPAPRLPKGAAWTTSFDERYTSSAVEATLGSKVECVYSLLSMLGSNDPLEMAKKFLELSGNAQSCATLRRSGCMPLLVQMMHAPDNDQEVRKCAGQALHNVVHSHPDEKAGRREAKVLRLLDQIVDYCSFLKTLLQSGGEAIADDSDRHPLAAISSLMKVSFDEEHRHAMCELGALHAIPNLVHLDHAVHGPKPEDQCCNSLRRYALMALTNLTFGDENNKALLCGQKQFMEALVAQLDSAPDDLLQVTASVLRNLSWRADSNMKAVLNEIGTVTALALAAMRNRSENTLKAILSALWNLSAHCSTNKAEFCAVDGALAFLVGMLSYEGPSKTLKIIENAGGILRNVSSHIAVCEPYRQILRQHNCLAILLQQLKSESLTVVSNSCGTLWNLSARSAEDQKFLWDNGAVPMLRSLIHSKHAMISEGSSSALKNLLNFRPAVQNHHQLDPIARSMGLKALPTLEARKAKALQQELGERHTAETCDNLDTGGKLDKERASSSSRRHPAPRLTRSAMLTKSESRDSVYSAKSDCAYDHLIRSASASDAHRKVKPKITDFDLEMEQDTEATEEQPIDYSVKYSENATKTSTYQETDLDQPTDFSLRYAENQIESDLDISGPAGGQKSTITPPAETVPEKSEGQEILLILDDSVKCYQTEDTPYVISNAASVTDLRVAAKADAEAEVKPEVREVTSKEGAPKKLPKLSQCGSGSYTPEKPINYCEEGTPGYFSRYDSLSSLDESGKANQAIVGTDADIKPKLEKQEEQESQPAEQVLTKPPTQANSALETPLMFSRRSSMDSLVHDPDVDVANCDDKSSVVSDFSRLASGVISPSEIPDSPTQSMPQSPRRNSVAGSGQNVDSPPVVIPASLQPLRSVFEDDLSSFNVEHTPAQFSTATSLSNLSIVDDEKAPASVAEEDNEDELLLANCINMGMQRKPTEAVKSTVVNSEVDVAEETIRSYCTEDTPALLSKVPSNTNLSVISMSSTDPKDATAGQAQMYAHQLSDDVSSNASDCGGASGHLLQQCIRDGMKKPLGEATSDPIAMLRRGGNELPGYLPSADEMNKFLVEDSPCNFSVVSGLSNLTVGSSLVGPAVQLKETEPSSADQNPEMKAKPGKQEQVRRPPHWQDDSLSSLSIDSEDDTNLLSQAIAAGCNRPKSNLGFSSNGKRSSSLSSSQPIAINAATSASSLNSAMTVRKSQQQESYSSVDSSDSNDNQSKSLFELCILKGMYKTKEPGARAQQMQEQPIVGSSSVQSNPSLKQFDSLPVQLPSSGQVKRQRHHHHHHHHRERERERKDEKLLQECINTGISKKINAVPKNVLATSAAALEPCHPMAATTSASALSTAAPDVEQKAHATSNPQQQSSTHPSSHILPNPIDAIATVTDTVRSPAAPNQGNGNASQNGLETATGSKDLDSEDRSSDESNQSFIMETMVRLDSALNETCISGASEKHKDPDLMLKSVERLTMEFVTSAEQLRSSSHNHSSSNSHKNNSSNNTWNESTCPNDVSFPSVSQTAPVLASLSLDEDATEARSLHELIEITPTNEQQPESLEGETDTLVNGHADSYSGSSGGLNFQLGGQVQNAGVRLEPQRLLFNGTSASIMTNSTMIAFEARALAENLLQPAATDDDTTEMTFSLNSLDLDNIRPPSGMESLNSCYQDHSQPSSLRQAMPSKSPRFARKMFPANLVARRALGHLAGSAESVNSSCNLLDNIKPPSLMDELLDSMISVDSIQSEVADGEQDCSMATTISVSNYETAACDDQTMTVLQSCFDEDEDATMNDYSSAESTPKHGSTPSPNRRSLTPKDKRRLTKDRFKTYTIATSCEMEAPEANETLQIEIVEAAVPVATPSPRANGRRRGSAERYKTQLIECPLALIQPQPDDCPSEQLSSIRAMMQQFTFITDINIGHSQETCESTDHPEDAGESPECDQNSETESCDGQEPDQLPPPPSIVDLRTSVVKPTTLEPATAVKLVRGRKKPAYVSPYSMQSQRNSNNAAPSKKKTLSPTIAKRSLVPGGSGVRLPAKKKPTPPPEPAPARLERQGTFVKDEPTNSNVQVPVVETKPAQTSPTHRASKLPTKKGTASGGSPSKAGSPKRIPLAPARRMTPQRANTSLRLAAGKSHAASRVVSGRVSSTTPPSRSNSNLNGSSAAAAAAAKINHAQSRIANIWKRVDEAKTKQSSSNLRTQKTKSSNMLNANGTKPTLLRSSTFDNTPSTAGGVKSKLPVVGARK

Target ORF information:

RefSeq Version	NM_001276117.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster APC-like (Apc), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276117.1

ORF Insert Sequence:

ATGTTGGAGC CAACGCTGAC GCCCGATCTG GAGGAGGGCA TCGCCGAGCT CAGTCTGGAG 
GACGTGGATG TGGACTCGGT GCCAGAGCGT AAGCCGCACT TCCTCGACTA CGACGCAGTG 
CCCCCGCCAG ACTATGAAGA AGGCTTTGGG GCCAGTTCCG GGGAGCAGCT AAAGATGGAC 
AAGAAGTACG AGCGGGATGG CGAAGTGAGC GACTACGAAC TGGCCGCCAG TGGCTATACC 
AAGAAGGAGT TCACCCAGGA CGACAACACG CTGCACTTCA CCCAGAGCGC AGTCGGACTG 
GGATCCGGAG CCGTTGGTAA AAAACCAGCA GCCAAGCATT TTCTGGACGA GAACCCCATT 
CCGCCGGACT ACATGTTGGC CCAGGAGCTG CGGGAGATGA GAGAACATCG CAGCCTGGAC 
AGGAACTTCG AGCGACAGTC GGCGCAGCAG CAGCAGCTGG ATGAATTGCC TCCGAGGAAT 
GGAGGCGGCT CTCCAGCCAG CGCAGGTCGT CCATCGCGCA GCAAGGAACC ATCTTACACG 
CTCTCCCGAT TTCTGGACGG AGATGCACCT GCCCCTGCTC CAAGGCTGCC CAAGGGAGCT 
GCCTGGACGA CCAGCTTCGA TGAGCGATAC ACCTCCTCGG CAGTGGAAGC CACCTTGGGA 
TCCAAGGTGG AGTGTGTTTA CTCACTGCTC TCCATGTTGG GCTCAAATGA TCCTCTCGAA 
ATGGCCAAAA AGTTCCTGGA GCTGTCCGGA AATGCTCAAA GTTGTGCAAC TCTTAGACGC 
TCTGGTTGCA TGCCGCTTCT GGTGCAGATG ATGCATGCAC CGGACAACGA CCAGGAGGTG 
AGGAAGTGTG CTGGACAGGC GCTGCACAAT GTGGTGCACA GCCATCCGGA CGAGAAGGCT 
GGCAGAAGGG AAGCCAAGGT GCTCCGACTG CTTGACCAGA TCGTTGACTA CTGCTCCTTC 
CTGAAGACGC TGCTCCAGAG TGGCGGAGAG GCTATTGCAG ATGACTCCGA CCGTCATCCG 
CTGGCCGCCA TCTCTTCACT GATGAAGGTG AGCTTCGATG AGGAGCACCG ACACGCTATG 
TGCGAGCTGG GGGCATTGCA CGCGATCCCC AATCTGGTTC ATTTGGATCA CGCCGTTCAT 
GGACCCAAAC CGGAGGATCA GTGCTGCAAT TCTCTGCGAA GATACGCTTT GATGGCCCTG 
ACCAATCTGA CCTTTGGGGA TGAGAACAAC AAGGCGCTGC TGTGTGGTCA AAAGCAGTTC 
ATGGAGGCCC TGGTGGCCCA ATTGGACTCG GCCCCGGACG ATCTGCTCCA GGTGACGGCC 
AGCGTGCTGC GCAATCTCTC CTGGCGAGCG GACAGCAACA TGAAGGCAGT GCTGAATGAG 
ATTGGAACAG TGACAGCTCT GGCCCTGGCG GCCATGCGGA ATAGGAGTGA GAACACCCTG 
AAGGCCATAC TCTCGGCCCT GTGGAATCTC TCGGCGCACT GCAGCACCAA CAAGGCGGAG 
TTCTGTGCCG TAGATGGGGC CTTGGCATTC CTAGTAGGAA TGCTAAGTTA CGAAGGTCCC 
AGCAAAACAT TGAAGATCAT TGAGAATGCG GGAGGGATCC TGCGGAACGT GTCCAGCCAC 
ATTGCTGTTT GTGAACCTTA TCGGCAGATC CTGCGCCAGC ACAACTGCCT GGCTATTCTG 
CTGCAGCAAT TGAAGTCCGA AAGCCTTACC GTGGTCAGCA ACTCCTGTGG AACTCTATGG 
AACCTGTCAG CTCGCTCTGC CGAGGATCAG AAGTTCCTGT GGGACAATGG AGCAGTGCCC 
ATGCTCCGTT CGTTGATCCA CTCCAAACAC GCCATGATCT CAGAGGGCAG CTCTTCGGCC 
TTGAAGAACC TTTTGAACTT CCGACCAGCT GTTCAAAATC ATCACCAGCT GGATCCAATT 
GCCCGCTCCA TGGGCCTCAA AGCATTGCCC ACTCTGGAGG CACGAAAAGC CAAGGCATTG 
CAACAGGAGC TGGGTGAGCG TCACACGGCC GAGACGTGCG ATAACTTGGA CACGGGCGGA 
AAACTGGATA AAGAACGAGC CTCCAGTTCA TCCAGACGTC ATCCTGCGCC ACGCTTAACC 
CGATCGGCAA TGCTAACGAA GAGCGAGAGC AGGGATTCGG TGTACTCGGC CAAATCCGAT 
TGCGCTTACG ATCATCTAAT TCGCTCAGCC TCTGCTTCAG ATGCCCATCG AAAGGTGAAG 
CCCAAGATCA CGGACTTCGA TCTGGAAATG GAGCAGGACA CGGAGGCAAC CGAGGAACAA 
CCCATTGACT ACTCGGTGAA GTACAGCGAG AATGCCACCA AGACCTCAAC ATATCAGGAA 
ACGGACCTGG ATCAGCCCAC GGACTTCAGC CTTCGCTACG CTGAGAACCA AATTGAATCC 
GACCTGGACA TATCAGGACC AGCAGGCGGA CAAAAGAGCA CTATCACTCC TCCTGCTGAA 
ACAGTTCCCG AGAAATCCGA GGGCCAGGAA ATATTGTTGA TCCTAGACGA CAGTGTTAAG 
TGCTACCAGA CGGAGGATAC CCCTTATGTT ATATCCAATG CGGCTTCAGT CACAGATTTA 
CGAGTCGCTG CTAAAGCGGA TGCAGAAGCG GAAGTCAAGC CAGAAGTTCG CGAGGTGACT 
TCCAAAGAAG GAGCTCCCAA AAAGCTTCCC AAGCTTTCGC AGTGTGGCTC CGGTTCGTAC 
ACTCCAGAGA AACCCATTAA CTACTGCGAG GAGGGAACTC CTGGGTACTT TAGTCGCTAC 
GATTCACTGA GCAGCTTGGA TGAATCCGGT AAGGCTAACC AGGCAATAGT AGGAACGGAT 
GCGGATATCA AGCCCAAATT AGAGAAGCAG GAGGAACAGG AATCCCAGCC AGCAGAGCAG 
GTCCTGACCA AACCTCCCAC CCAGGCAAAC TCGGCATTGG AAACCCCGTT GATGTTCTCG 
CGACGCAGTT CCATGGACTC CCTAGTCCAC GATCCGGATG TCGATGTGGC CAACTGCGAT 
GACAAGAGCT CGGTGGTTAG TGACTTCAGT CGCTTGGCCA GTGGGGTAAT ATCCCCATCC 
GAGATCCCAG ATTCCCCCAC ACAAAGTATG CCCCAATCTC CACGACGAAA TAGTGTCGCT 
GGTTCGGGGC AGAATGTCGA TTCTCCACCG GTTGTAATCC CGGCCAGCCT ACAACCATTA 
CGCAGCGTTT TCGAGGACGA CCTGAGTAGC TTCAACGTGG AGCATACACC TGCTCAGTTC 
TCCACGGCCA CCAGTTTGAG CAACCTGAGC ATAGTGGACG ATGAAAAGGC ACCGGCCAGT 
GTGGCCGAGG AAGACAACGA AGATGAGCTC CTTTTGGCCA ACTGCATCAA CATGGGTATG 
CAGCGGAAGC CAACTGAGGC TGTGAAATCG ACAGTTGTGA ACTCAGAAGT GGACGTGGCT 
GAGGAGACCA TTCGTAGCTA CTGCACCGAA GATACGCCAG CATTGCTCTC CAAAGTACCA 
AGTAACACTA ACCTTTCGGT TATCTCCATG AGCTCCACCG ATCCCAAGGA TGCCACGGCT 
GGTCAGGCAC AGATGTACGC TCATCAGCTA TCCGACGATG TATCTTCCAA TGCATCGGAC 
TGCGGTGGAG CCTCTGGTCA CCTGCTACAG CAGTGCATCC GGGATGGCAT GAAGAAACCC 
CTCGGTGAAG CCACTTCAGA TCCTATAGCC ATGCTTCGAC GAGGCGGAAA CGAATTGCCT 
GGCTATCTGC CCTCTGCCGA TGAAATGAAC AAGTTTTTGG TGGAGGACAG TCCCTGTAAC 
TTCTCAGTGG TCTCTGGTTT ATCTAATCTC ACCGTGGGAT CCAGTCTGGT TGGGCCAGCC 
GTACAGCTCA AGGAGACCGA ACCATCCAGT GCGGATCAGA ATCCTGAGAT GAAGGCGAAG 
CCTGGCAAAC AGGAGCAAGT CCGAAGACCG CCGCATTGGC AAGATGATTC GCTGAGTTCT 
CTATCCATTG ACTCGGAAGA TGACACCAAC TTACTGAGTC AGGCTATTGC CGCTGGTTGC 
AATAGACCGA AATCGAATCT TGGCTTCAGC TCGAACGGCA AGCGCTCCAG CTCGCTGAGC 
TCCTCGCAGC CAATAGCCAT CAATGCGGCC ACCTCGGCCA GCTCCCTGAA TTCGGCGATG 
ACAGTACGCA AGAGCCAACA GCAGGAGTCC TACAGTTCCG TGGACTCTAG CGATTCTAAC 
GACAACCAGT CAAAGTCGCT CTTCGAGCTG TGCATCCTCA AGGGCATGTA CAAGACGAAG 
GAACCCGGCG CCCGGGCCCA GCAGATGCAG GAGCAACCTA TAGTGGGTTC ATCCTCCGTC 
CAGTCGAATC CCAGTCTAAA GCAGTTCGAT TCGCTGCCGG TGCAGCTGCC ATCTAGTGGA 
CAGGTCAAGC GGCAGCGCCA TCATCATCAT CACCATCATC ATAGGGAGCG AGAGCGAGAA 
CGCAAGGACG AGAAGCTGCT GCAGGAGTGC ATCAATACGG GCATCTCGAA GAAGATCAAC 
GCCGTGCCAA AAAACGTCCT GGCTACGTCT GCGGCTGCAT TGGAACCGTG TCACCCAATG 
GCAGCTACTA CATCAGCAAG TGCCCTCAGC ACAGCAGCTC CAGACGTAGA GCAGAAAGCG 
CACGCCACCA GCAATCCGCA GCAGCAGTCG TCCACGCACC CGAGCAGCCA CATCCTCCCG 
AATCCTATCG ACGCCATCGC CACCGTCACC GACACAGTAA GAAGTCCAGC AGCTCCAAAT 
CAAGGGAACG GGAACGCGAG TCAGAACGGT CTCGAGACCG CTACTGGGTC TAAGGACTTG 
GATTCGGAAG ATCGATCCAG CGATGAATCA AATCAGTCCT TCATCATGGA AACCATGGTC 
AGGTTGGACA GTGCTCTCAA CGAAACGTGC ATCTCCGGAG CAAGCGAGAA GCACAAGGAT 
CCCGACCTGA TGCTGAAGTC TGTGGAGAGA CTGACCATGG AGTTTGTTAC GTCGGCGGAG 
CAGTTGCGCA GCAGCAGCCA CAACCACAGC AGCAGCAACA GCCACAAGAA CAACAGTAGC 
AACAACACCT GGAACGAGAG CACCTGTCCC AATGATGTGA GTTTCCCCAG CGTCAGTCAA 
ACGGCACCGG TTCTGGCCTC CTTGAGCTTG GATGAAGATG CCACGGAGGC GAGATCCCTG 
CACGAGTTGA TTGAGATAAC GCCCACGAAT GAACAGCAAC CGGAATCGCT CGAAGGGGAA 
ACTGATACCC TGGTTAATGG TCATGCTGAT AGCTACTCAG GATCGTCTGG AGGTCTTAAC 
TTCCAGTTGG GTGGGCAGGT GCAGAATGCT GGCGTTAGAT TGGAGCCGCA AAGACTATTA 
TTTAATGGAA CTAGTGCCTC CATTATGACA AACTCGACCA TGATTGCGTT CGAAGCGCGA 
GCTCTGGCGG AAAATCTACT CCAGCCCGCT GCCACTGACG ATGACACCAC GGAGATGACC 
TTCAGCTTGA ACAGTTTGGA TCTGGATAAT ATTCGTCCGC CGTCGGGAAT GGAATCGCTG 
AACAGCTGCT ACCAGGATCA CTCCCAGCCT AGTTCTCTGC GCCAGGCGAT GCCCAGCAAG 
AGTCCACGCT TCGCGCGAAA AATGTTTCCA GCCAATTTGG TAGCCAGACG AGCACTGGGT 
CACTTAGCTG GCAGTGCGGA GAGCGTGAAC TCCAGCTGCA ACCTGCTGGA CAACATCAAG 
CCTCCGTCAC TGATGGATGA GCTGCTGGAC TCGATGATCA GTGTGGACAG CATTCAATCT 
GAGGTGGCCG ATGGCGAGCA GGACTGCAGC ATGGCCACCA CCATTTCCGT GTCCAACTAT 
GAGACGGCTG CTTGCGATGA TCAGACGATG ACCGTGCTCC AAAGTTGTTT CGACGAGGAT 
GAGGATGCCA CAATGAATGA CTACAGCTCG GCCGAGTCCA CACCCAAGCA CGGATCTACT 
CCATCACCCA ATCGTCGTTC CCTCACACCG AAAGACAAGC GACGTCTCAC AAAGGATCGC 
TTCAAGACCT ACACCATAGC CACCAGCTGC GAGATGGAAG CACCAGAGGC CAATGAAACT 
TTGCAGATAG AAATTGTGGA AGCTGCCGTT CCTGTGGCCA CGCCATCGCC ACGCGCCAAT 
GGAAGGAGAA GGGGCAGTGC GGAGCGGTAC AAGACCCAAC TGATTGAATG TCCCCTGGCT 
TTGATACAAC CCCAACCCGA TGATTGCCCA AGCGAACAGC TCTCCTCAAT TCGAGCAATG 
ATGCAACAGT TCACTTTCAT CACGGACATT AACATTGGAC ACAGTCAGGA GACTTGCGAG 
AGTACTGATC ATCCAGAAGA CGCAGGGGAA TCCCCGGAGT GCGATCAGAA CTCTGAGACG 
GAGTCGTGCG ATGGTCAGGA ACCGGATCAG CTGCCTCCTC CTCCGTCAAT TGTGGACCTT 
AGAACATCAG TCGTTAAGCC CACAACCTTG GAGCCCGCCA CGGCCGTAAA ACTGGTGCGA 
GGTCGCAAGA AACCAGCCTA TGTCTCACCC TACAGCATGC AAAGTCAACG GAATAGCAAC 
AATGCCGCCC CGTCCAAGAA GAAAACCCTA TCTCCAACAA TTGCCAAACG AAGTCTGGTG 
CCCGGCGGTT CAGGAGTTAG GTTACCAGCG AAAAAGAAGC CGACGCCGCC GCCTGAGCCG 
GCACCCGCAC GCTTGGAACG CCAGGGAACC TTTGTCAAGG ACGAGCCCAC TAACTCCAAT 
GTTCAAGTGC CCGTGGTGGA GACAAAACCG GCTCAAACCA GTCCCACTCA CCGCGCCTCG 
AAGTTACCCA CAAAGAAAGG CACGGCCAGC GGTGGGTCAC CATCGAAAGC GGGCTCTCCC 
AAGAGGATTC CCTTGGCGCC GGCCCGCCGA ATGACGCCTC AAAGGGCAAA CACCAGTCTG 
CGACTAGCTG CGGGCAAATC TCACGCTGCC TCACGTGTGG TCTCCGGCCG CGTGTCGTCC 
ACTACTCCAC CATCCCGCAG CAACTCCAAC CTAAATGGAA GCAGTGCAGC CGCAGCAGCT 
GCGGCCAAGA TCAACCATGC GCAAAGTCGT ATCGCCAACA TCTGGAAGAG AGTGGACGAG 
GCCAAAACCA AGCAATCGTC ATCCAACCTG AGGACGCAGA AAACCAAATC TTCCAACATG 
CTCAACGCAA ATGGAACCAA GCCGACGCTC CTGCGCAGCT CCACGTTTGA CAATACCCCC 
AGCACCGCTG GAGGAGTCAA GTCCAAGCTG CCAGTGGTGG GCGCGCGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( NM_057804.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477152.1 Drosophila melanogaster APC-like (Apc), transcript variant A, mRNA.

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CloneID	OFa06856
Clone ID Related Accession (Same CDS sequence)	NM_001276117.1 , NM_057804.3
Accession Version	NM_057804.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	APC-like, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057804.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGGAGC CAACGCTGAC GCCCGATCTG GAGGAGGGCA TCGCCGAGCT CAGTCTGGAG 
GACGTGGATG TGGACTCGGT GCCAGAGCGT AAGCCGCACT TCCTCGACTA CGACGCAGTG 
CCCCCGCCAG ACTATGAAGA AGGCTTTGGG GCCAGTTCCG GGGAGCAGCT AAAGATGGAC 
AAGAAGTACG AGCGGGATGG CGAAGTGAGC GACTACGAAC TGGCCGCCAG TGGCTATACC 
AAGAAGGAGT TCACCCAGGA CGACAACACG CTGCACTTCA CCCAGAGCGC AGTCGGACTG 
GGATCCGGAG CCGTTGGTAA AAAACCAGCA GCCAAGCATT TTCTGGACGA GAACCCCATT 
CCGCCGGACT ACATGTTGGC CCAGGAGCTG CGGGAGATGA GAGAACATCG CAGCCTGGAC 
AGGAACTTCG AGCGACAGTC GGCGCAGCAG CAGCAGCTGG ATGAATTGCC TCCGAGGAAT 
GGAGGCGGCT CTCCAGCCAG CGCAGGTCGT CCATCGCGCA GCAAGGAACC ATCTTACACG 
CTCTCCCGAT TTCTGGACGG AGATGCACCT GCCCCTGCTC CAAGGCTGCC CAAGGGAGCT 
GCCTGGACGA CCAGCTTCGA TGAGCGATAC ACCTCCTCGG CAGTGGAAGC CACCTTGGGA 
TCCAAGGTGG AGTGTGTTTA CTCACTGCTC TCCATGTTGG GCTCAAATGA TCCTCTCGAA 
ATGGCCAAAA AGTTCCTGGA GCTGTCCGGA AATGCTCAAA GTTGTGCAAC TCTTAGACGC 
TCTGGTTGCA TGCCGCTTCT GGTGCAGATG ATGCATGCAC CGGACAACGA CCAGGAGGTG 
AGGAAGTGTG CTGGACAGGC GCTGCACAAT GTGGTGCACA GCCATCCGGA CGAGAAGGCT 
GGCAGAAGGG AAGCCAAGGT GCTCCGACTG CTTGACCAGA TCGTTGACTA CTGCTCCTTC 
CTGAAGACGC TGCTCCAGAG TGGCGGAGAG GCTATTGCAG ATGACTCCGA CCGTCATCCG 
CTGGCCGCCA TCTCTTCACT GATGAAGGTG AGCTTCGATG AGGAGCACCG ACACGCTATG 
TGCGAGCTGG GGGCATTGCA CGCGATCCCC AATCTGGTTC ATTTGGATCA CGCCGTTCAT 
GGACCCAAAC CGGAGGATCA GTGCTGCAAT TCTCTGCGAA GATACGCTTT GATGGCCCTG 
ACCAATCTGA CCTTTGGGGA TGAGAACAAC AAGGCGCTGC TGTGTGGTCA AAAGCAGTTC 
ATGGAGGCCC TGGTGGCCCA ATTGGACTCG GCCCCGGACG ATCTGCTCCA GGTGACGGCC 
AGCGTGCTGC GCAATCTCTC CTGGCGAGCG GACAGCAACA TGAAGGCAGT GCTGAATGAG 
ATTGGAACAG TGACAGCTCT GGCCCTGGCG GCCATGCGGA ATAGGAGTGA GAACACCCTG 
AAGGCCATAC TCTCGGCCCT GTGGAATCTC TCGGCGCACT GCAGCACCAA CAAGGCGGAG 
TTCTGTGCCG TAGATGGGGC CTTGGCATTC CTAGTAGGAA TGCTAAGTTA CGAAGGTCCC 
AGCAAAACAT TGAAGATCAT TGAGAATGCG GGAGGGATCC TGCGGAACGT GTCCAGCCAC 
ATTGCTGTTT GTGAACCTTA TCGGCAGATC CTGCGCCAGC ACAACTGCCT GGCTATTCTG 
CTGCAGCAAT TGAAGTCCGA AAGCCTTACC GTGGTCAGCA ACTCCTGTGG AACTCTATGG 
AACCTGTCAG CTCGCTCTGC CGAGGATCAG AAGTTCCTGT GGGACAATGG AGCAGTGCCC 
ATGCTCCGTT CGTTGATCCA CTCCAAACAC GCCATGATCT CAGAGGGCAG CTCTTCGGCC 
TTGAAGAACC TTTTGAACTT CCGACCAGCT GTTCAAAATC ATCACCAGCT GGATCCAATT 
GCCCGCTCCA TGGGCCTCAA AGCATTGCCC ACTCTGGAGG CACGAAAAGC CAAGGCATTG 
CAACAGGAGC TGGGTGAGCG TCACACGGCC GAGACGTGCG ATAACTTGGA CACGGGCGGA 
AAACTGGATA AAGAACGAGC CTCCAGTTCA TCCAGACGTC ATCCTGCGCC ACGCTTAACC 
CGATCGGCAA TGCTAACGAA GAGCGAGAGC AGGGATTCGG TGTACTCGGC CAAATCCGAT 
TGCGCTTACG ATCATCTAAT TCGCTCAGCC TCTGCTTCAG ATGCCCATCG AAAGGTGAAG 
CCCAAGATCA CGGACTTCGA TCTGGAAATG GAGCAGGACA CGGAGGCAAC CGAGGAACAA 
CCCATTGACT ACTCGGTGAA GTACAGCGAG AATGCCACCA AGACCTCAAC ATATCAGGAA 
ACGGACCTGG ATCAGCCCAC GGACTTCAGC CTTCGCTACG CTGAGAACCA AATTGAATCC 
GACCTGGACA TATCAGGACC AGCAGGCGGA CAAAAGAGCA CTATCACTCC TCCTGCTGAA 
ACAGTTCCCG AGAAATCCGA GGGCCAGGAA ATATTGTTGA TCCTAGACGA CAGTGTTAAG 
TGCTACCAGA CGGAGGATAC CCCTTATGTT ATATCCAATG CGGCTTCAGT CACAGATTTA 
CGAGTCGCTG CTAAAGCGGA TGCAGAAGCG GAAGTCAAGC CAGAAGTTCG CGAGGTGACT 
TCCAAAGAAG GAGCTCCCAA AAAGCTTCCC AAGCTTTCGC AGTGTGGCTC CGGTTCGTAC 
ACTCCAGAGA AACCCATTAA CTACTGCGAG GAGGGAACTC CTGGGTACTT TAGTCGCTAC 
GATTCACTGA GCAGCTTGGA TGAATCCGGT AAGGCTAACC AGGCAATAGT AGGAACGGAT 
GCGGATATCA AGCCCAAATT AGAGAAGCAG GAGGAACAGG AATCCCAGCC AGCAGAGCAG 
GTCCTGACCA AACCTCCCAC CCAGGCAAAC TCGGCATTGG AAACCCCGTT GATGTTCTCG 
CGACGCAGTT CCATGGACTC CCTAGTCCAC GATCCGGATG TCGATGTGGC CAACTGCGAT 
GACAAGAGCT CGGTGGTTAG TGACTTCAGT CGCTTGGCCA GTGGGGTAAT ATCCCCATCC 
GAGATCCCAG ATTCCCCCAC ACAAAGTATG CCCCAATCTC CACGACGAAA TAGTGTCGCT 
GGTTCGGGGC AGAATGTCGA TTCTCCACCG GTTGTAATCC CGGCCAGCCT ACAACCATTA 
CGCAGCGTTT TCGAGGACGA CCTGAGTAGC TTCAACGTGG AGCATACACC TGCTCAGTTC 
TCCACGGCCA CCAGTTTGAG CAACCTGAGC ATAGTGGACG ATGAAAAGGC ACCGGCCAGT 
GTGGCCGAGG AAGACAACGA AGATGAGCTC CTTTTGGCCA ACTGCATCAA CATGGGTATG 
CAGCGGAAGC CAACTGAGGC TGTGAAATCG ACAGTTGTGA ACTCAGAAGT GGACGTGGCT 
GAGGAGACCA TTCGTAGCTA CTGCACCGAA GATACGCCAG CATTGCTCTC CAAAGTACCA 
AGTAACACTA ACCTTTCGGT TATCTCCATG AGCTCCACCG ATCCCAAGGA TGCCACGGCT 
GGTCAGGCAC AGATGTACGC TCATCAGCTA TCCGACGATG TATCTTCCAA TGCATCGGAC 
TGCGGTGGAG CCTCTGGTCA CCTGCTACAG CAGTGCATCC GGGATGGCAT GAAGAAACCC 
CTCGGTGAAG CCACTTCAGA TCCTATAGCC ATGCTTCGAC GAGGCGGAAA CGAATTGCCT 
GGCTATCTGC CCTCTGCCGA TGAAATGAAC AAGTTTTTGG TGGAGGACAG TCCCTGTAAC 
TTCTCAGTGG TCTCTGGTTT ATCTAATCTC ACCGTGGGAT CCAGTCTGGT TGGGCCAGCC 
GTACAGCTCA AGGAGACCGA ACCATCCAGT GCGGATCAGA ATCCTGAGAT GAAGGCGAAG 
CCTGGCAAAC AGGAGCAAGT CCGAAGACCG CCGCATTGGC AAGATGATTC GCTGAGTTCT 
CTATCCATTG ACTCGGAAGA TGACACCAAC TTACTGAGTC AGGCTATTGC CGCTGGTTGC 
AATAGACCGA AATCGAATCT TGGCTTCAGC TCGAACGGCA AGCGCTCCAG CTCGCTGAGC 
TCCTCGCAGC CAATAGCCAT CAATGCGGCC ACCTCGGCCA GCTCCCTGAA TTCGGCGATG 
ACAGTACGCA AGAGCCAACA GCAGGAGTCC TACAGTTCCG TGGACTCTAG CGATTCTAAC 
GACAACCAGT CAAAGTCGCT CTTCGAGCTG TGCATCCTCA AGGGCATGTA CAAGACGAAG 
GAACCCGGCG CCCGGGCCCA GCAGATGCAG GAGCAACCTA TAGTGGGTTC ATCCTCCGTC 
CAGTCGAATC CCAGTCTAAA GCAGTTCGAT TCGCTGCCGG TGCAGCTGCC ATCTAGTGGA 
CAGGTCAAGC GGCAGCGCCA TCATCATCAT CACCATCATC ATAGGGAGCG AGAGCGAGAA 
CGCAAGGACG AGAAGCTGCT GCAGGAGTGC ATCAATACGG GCATCTCGAA GAAGATCAAC 
GCCGTGCCAA AAAACGTCCT GGCTACGTCT GCGGCTGCAT TGGAACCGTG TCACCCAATG 
GCAGCTACTA CATCAGCAAG TGCCCTCAGC ACAGCAGCTC CAGACGTAGA GCAGAAAGCG 
CACGCCACCA GCAATCCGCA GCAGCAGTCG TCCACGCACC CGAGCAGCCA CATCCTCCCG 
AATCCTATCG ACGCCATCGC CACCGTCACC GACACAGTAA GAAGTCCAGC AGCTCCAAAT 
CAAGGGAACG GGAACGCGAG TCAGAACGGT CTCGAGACCG CTACTGGGTC TAAGGACTTG 
GATTCGGAAG ATCGATCCAG CGATGAATCA AATCAGTCCT TCATCATGGA AACCATGGTC 
AGGTTGGACA GTGCTCTCAA CGAAACGTGC ATCTCCGGAG CAAGCGAGAA GCACAAGGAT 
CCCGACCTGA TGCTGAAGTC TGTGGAGAGA CTGACCATGG AGTTTGTTAC GTCGGCGGAG 
CAGTTGCGCA GCAGCAGCCA CAACCACAGC AGCAGCAACA GCCACAAGAA CAACAGTAGC 
AACAACACCT GGAACGAGAG CACCTGTCCC AATGATGTGA GTTTCCCCAG CGTCAGTCAA 
ACGGCACCGG TTCTGGCCTC CTTGAGCTTG GATGAAGATG CCACGGAGGC GAGATCCCTG 
CACGAGTTGA TTGAGATAAC GCCCACGAAT GAACAGCAAC CGGAATCGCT CGAAGGGGAA 
ACTGATACCC TGGTTAATGG TCATGCTGAT AGCTACTCAG GATCGTCTGG AGGTCTTAAC 
TTCCAGTTGG GTGGGCAGGT GCAGAATGCT GGCGTTAGAT TGGAGCCGCA AAGACTATTA 
TTTAATGGAA CTAGTGCCTC CATTATGACA AACTCGACCA TGATTGCGTT CGAAGCGCGA 
GCTCTGGCGG AAAATCTACT CCAGCCCGCT GCCACTGACG ATGACACCAC GGAGATGACC 
TTCAGCTTGA ACAGTTTGGA TCTGGATAAT ATTCGTCCGC CGTCGGGAAT GGAATCGCTG 
AACAGCTGCT ACCAGGATCA CTCCCAGCCT AGTTCTCTGC GCCAGGCGAT GCCCAGCAAG 
AGTCCACGCT TCGCGCGAAA AATGTTTCCA GCCAATTTGG TAGCCAGACG AGCACTGGGT 
CACTTAGCTG GCAGTGCGGA GAGCGTGAAC TCCAGCTGCA ACCTGCTGGA CAACATCAAG 
CCTCCGTCAC TGATGGATGA GCTGCTGGAC TCGATGATCA GTGTGGACAG CATTCAATCT 
GAGGTGGCCG ATGGCGAGCA GGACTGCAGC ATGGCCACCA CCATTTCCGT GTCCAACTAT 
GAGACGGCTG CTTGCGATGA TCAGACGATG ACCGTGCTCC AAAGTTGTTT CGACGAGGAT 
GAGGATGCCA CAATGAATGA CTACAGCTCG GCCGAGTCCA CACCCAAGCA CGGATCTACT 
CCATCACCCA ATCGTCGTTC CCTCACACCG AAAGACAAGC GACGTCTCAC AAAGGATCGC 
TTCAAGACCT ACACCATAGC CACCAGCTGC GAGATGGAAG CACCAGAGGC CAATGAAACT 
TTGCAGATAG AAATTGTGGA AGCTGCCGTT CCTGTGGCCA CGCCATCGCC ACGCGCCAAT 
GGAAGGAGAA GGGGCAGTGC GGAGCGGTAC AAGACCCAAC TGATTGAATG TCCCCTGGCT 
TTGATACAAC CCCAACCCGA TGATTGCCCA AGCGAACAGC TCTCCTCAAT TCGAGCAATG 
ATGCAACAGT TCACTTTCAT CACGGACATT AACATTGGAC ACAGTCAGGA GACTTGCGAG 
AGTACTGATC ATCCAGAAGA CGCAGGGGAA TCCCCGGAGT GCGATCAGAA CTCTGAGACG 
GAGTCGTGCG ATGGTCAGGA ACCGGATCAG CTGCCTCCTC CTCCGTCAAT TGTGGACCTT 
AGAACATCAG TCGTTAAGCC CACAACCTTG GAGCCCGCCA CGGCCGTAAA ACTGGTGCGA 
GGTCGCAAGA AACCAGCCTA TGTCTCACCC TACAGCATGC AAAGTCAACG GAATAGCAAC 
AATGCCGCCC CGTCCAAGAA GAAAACCCTA TCTCCAACAA TTGCCAAACG AAGTCTGGTG 
CCCGGCGGTT CAGGAGTTAG GTTACCAGCG AAAAAGAAGC CGACGCCGCC GCCTGAGCCG 
GCACCCGCAC GCTTGGAACG CCAGGGAACC TTTGTCAAGG ACGAGCCCAC TAACTCCAAT 
GTTCAAGTGC CCGTGGTGGA GACAAAACCG GCTCAAACCA GTCCCACTCA CCGCGCCTCG 
AAGTTACCCA CAAAGAAAGG CACGGCCAGC GGTGGGTCAC CATCGAAAGC GGGCTCTCCC 
AAGAGGATTC CCTTGGCGCC GGCCCGCCGA ATGACGCCTC AAAGGGCAAA CACCAGTCTG 
CGACTAGCTG CGGGCAAATC TCACGCTGCC TCACGTGTGG TCTCCGGCCG CGTGTCGTCC 
ACTACTCCAC CATCCCGCAG CAACTCCAAC CTAAATGGAA GCAGTGCAGC CGCAGCAGCT 
GCGGCCAAGA TCAACCATGC GCAAAGTCGT ATCGCCAACA TCTGGAAGAG AGTGGACGAG 
GCCAAAACCA AGCAATCGTC ATCCAACCTG AGGACGCAGA AAACCAAATC TTCCAACATG 
CTCAACGCAA ATGGAACCAA GCCGACGCTC CTGCGCAGCT CCACGTTTGA CAATACCCCC 
AGCACCGCTG GAGGAGTCAA GTCCAAGCTG CCAGTGGTGG GCGCGCGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477152.1
CDS	254..7507
Translation	MLEPTLTPDLEEGIAELSLEDVDVDSVPERKPHFLDYDAVPPPDYEEGFGASSGEQLKMDKKYERDGEVSDYELAASGYTKKEFTQDDNTLHFTQSAVGLGSGAVGKKPAAKHFLDENPIPPDYMLAQELREMREHRSLDRNFERQSAQQQQLDELPPRNGGGSPASAGRPSRSKEPSYTLSRFLDGDAPAPAPRLPKGAAWTTSFDERYTSSAVEATLGSKVECVYSLLSMLGSNDPLEMAKKFLELSGNAQSCATLRRSGCMPLLVQMMHAPDNDQEVRKCAGQALHNVVHSHPDEKAGRREAKVLRLLDQIVDYCSFLKTLLQSGGEAIADDSDRHPLAAISSLMKVSFDEEHRHAMCELGALHAIPNLVHLDHAVHGPKPEDQCCNSLRRYALMALTNLTFGDENNKALLCGQKQFMEALVAQLDSAPDDLLQVTASVLRNLSWRADSNMKAVLNEIGTVTALALAAMRNRSENTLKAILSALWNLSAHCSTNKAEFCAVDGALAFLVGMLSYEGPSKTLKIIENAGGILRNVSSHIAVCEPYRQILRQHNCLAILLQQLKSESLTVVSNSCGTLWNLSARSAEDQKFLWDNGAVPMLRSLIHSKHAMISEGSSSALKNLLNFRPAVQNHHQLDPIARSMGLKALPTLEARKAKALQQELGERHTAETCDNLDTGGKLDKERASSSSRRHPAPRLTRSAMLTKSESRDSVYSAKSDCAYDHLIRSASASDAHRKVKPKITDFDLEMEQDTEATEEQPIDYSVKYSENATKTSTYQETDLDQPTDFSLRYAENQIESDLDISGPAGGQKSTITPPAETVPEKSEGQEILLILDDSVKCYQTEDTPYVISNAASVTDLRVAAKADAEAEVKPEVREVTSKEGAPKKLPKLSQCGSGSYTPEKPINYCEEGTPGYFSRYDSLSSLDESGKANQAIVGTDADIKPKLEKQEEQESQPAEQVLTKPPTQANSALETPLMFSRRSSMDSLVHDPDVDVANCDDKSSVVSDFSRLASGVISPSEIPDSPTQSMPQSPRRNSVAGSGQNVDSPPVVIPASLQPLRSVFEDDLSSFNVEHTPAQFSTATSLSNLSIVDDEKAPASVAEEDNEDELLLANCINMGMQRKPTEAVKSTVVNSEVDVAEETIRSYCTEDTPALLSKVPSNTNLSVISMSSTDPKDATAGQAQMYAHQLSDDVSSNASDCGGASGHLLQQCIRDGMKKPLGEATSDPIAMLRRGGNELPGYLPSADEMNKFLVEDSPCNFSVVSGLSNLTVGSSLVGPAVQLKETEPSSADQNPEMKAKPGKQEQVRRPPHWQDDSLSSLSIDSEDDTNLLSQAIAAGCNRPKSNLGFSSNGKRSSSLSSSQPIAINAATSASSLNSAMTVRKSQQQESYSSVDSSDSNDNQSKSLFELCILKGMYKTKEPGARAQQMQEQPIVGSSSVQSNPSLKQFDSLPVQLPSSGQVKRQRHHHHHHHHRERERERKDEKLLQECINTGISKKINAVPKNVLATSAAALEPCHPMAATTSASALSTAAPDVEQKAHATSNPQQQSSTHPSSHILPNPIDAIATVTDTVRSPAAPNQGNGNASQNGLETATGSKDLDSEDRSSDESNQSFIMETMVRLDSALNETCISGASEKHKDPDLMLKSVERLTMEFVTSAEQLRSSSHNHSSSNSHKNNSSNNTWNESTCPNDVSFPSVSQTAPVLASLSLDEDATEARSLHELIEITPTNEQQPESLEGETDTLVNGHADSYSGSSGGLNFQLGGQVQNAGVRLEPQRLLFNGTSASIMTNSTMIAFEARALAENLLQPAATDDDTTEMTFSLNSLDLDNIRPPSGMESLNSCYQDHSQPSSLRQAMPSKSPRFARKMFPANLVARRALGHLAGSAESVNSSCNLLDNIKPPSLMDELLDSMISVDSIQSEVADGEQDCSMATTISVSNYETAACDDQTMTVLQSCFDEDEDATMNDYSSAESTPKHGSTPSPNRRSLTPKDKRRLTKDRFKTYTIATSCEMEAPEANETLQIEIVEAAVPVATPSPRANGRRRGSAERYKTQLIECPLALIQPQPDDCPSEQLSSIRAMMQQFTFITDINIGHSQETCESTDHPEDAGESPECDQNSETESCDGQEPDQLPPPPSIVDLRTSVVKPTTLEPATAVKLVRGRKKPAYVSPYSMQSQRNSNNAAPSKKKTLSPTIAKRSLVPGGSGVRLPAKKKPTPPPEPAPARLERQGTFVKDEPTNSNVQVPVVETKPAQTSPTHRASKLPTKKGTASGGSPSKAGSPKRIPLAPARRMTPQRANTSLRLAAGKSHAASRVVSGRVSSTTPPSRSNSNLNGSSAAAAAAAKINHAQSRIANIWKRVDEAKTKQSSSNLRTQKTKSSNMLNANGTKPTLLRSSTFDNTPSTAGGVKSKLPVVGARK

Target ORF information:

RefSeq Version	NM_057804.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster APC-like (Apc), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057804.3

ORF Insert Sequence:

ATGTTGGAGC CAACGCTGAC GCCCGATCTG GAGGAGGGCA TCGCCGAGCT CAGTCTGGAG 
GACGTGGATG TGGACTCGGT GCCAGAGCGT AAGCCGCACT TCCTCGACTA CGACGCAGTG 
CCCCCGCCAG ACTATGAAGA AGGCTTTGGG GCCAGTTCCG GGGAGCAGCT AAAGATGGAC 
AAGAAGTACG AGCGGGATGG CGAAGTGAGC GACTACGAAC TGGCCGCCAG TGGCTATACC 
AAGAAGGAGT TCACCCAGGA CGACAACACG CTGCACTTCA CCCAGAGCGC AGTCGGACTG 
GGATCCGGAG CCGTTGGTAA AAAACCAGCA GCCAAGCATT TTCTGGACGA GAACCCCATT 
CCGCCGGACT ACATGTTGGC CCAGGAGCTG CGGGAGATGA GAGAACATCG CAGCCTGGAC 
AGGAACTTCG AGCGACAGTC GGCGCAGCAG CAGCAGCTGG ATGAATTGCC TCCGAGGAAT 
GGAGGCGGCT CTCCAGCCAG CGCAGGTCGT CCATCGCGCA GCAAGGAACC ATCTTACACG 
CTCTCCCGAT TTCTGGACGG AGATGCACCT GCCCCTGCTC CAAGGCTGCC CAAGGGAGCT 
GCCTGGACGA CCAGCTTCGA TGAGCGATAC ACCTCCTCGG CAGTGGAAGC CACCTTGGGA 
TCCAAGGTGG AGTGTGTTTA CTCACTGCTC TCCATGTTGG GCTCAAATGA TCCTCTCGAA 
ATGGCCAAAA AGTTCCTGGA GCTGTCCGGA AATGCTCAAA GTTGTGCAAC TCTTAGACGC 
TCTGGTTGCA TGCCGCTTCT GGTGCAGATG ATGCATGCAC CGGACAACGA CCAGGAGGTG 
AGGAAGTGTG CTGGACAGGC GCTGCACAAT GTGGTGCACA GCCATCCGGA CGAGAAGGCT 
GGCAGAAGGG AAGCCAAGGT GCTCCGACTG CTTGACCAGA TCGTTGACTA CTGCTCCTTC 
CTGAAGACGC TGCTCCAGAG TGGCGGAGAG GCTATTGCAG ATGACTCCGA CCGTCATCCG 
CTGGCCGCCA TCTCTTCACT GATGAAGGTG AGCTTCGATG AGGAGCACCG ACACGCTATG 
TGCGAGCTGG GGGCATTGCA CGCGATCCCC AATCTGGTTC ATTTGGATCA CGCCGTTCAT 
GGACCCAAAC CGGAGGATCA GTGCTGCAAT TCTCTGCGAA GATACGCTTT GATGGCCCTG 
ACCAATCTGA CCTTTGGGGA TGAGAACAAC AAGGCGCTGC TGTGTGGTCA AAAGCAGTTC 
ATGGAGGCCC TGGTGGCCCA ATTGGACTCG GCCCCGGACG ATCTGCTCCA GGTGACGGCC 
AGCGTGCTGC GCAATCTCTC CTGGCGAGCG GACAGCAACA TGAAGGCAGT GCTGAATGAG 
ATTGGAACAG TGACAGCTCT GGCCCTGGCG GCCATGCGGA ATAGGAGTGA GAACACCCTG 
AAGGCCATAC TCTCGGCCCT GTGGAATCTC TCGGCGCACT GCAGCACCAA CAAGGCGGAG 
TTCTGTGCCG TAGATGGGGC CTTGGCATTC CTAGTAGGAA TGCTAAGTTA CGAAGGTCCC 
AGCAAAACAT TGAAGATCAT TGAGAATGCG GGAGGGATCC TGCGGAACGT GTCCAGCCAC 
ATTGCTGTTT GTGAACCTTA TCGGCAGATC CTGCGCCAGC ACAACTGCCT GGCTATTCTG 
CTGCAGCAAT TGAAGTCCGA AAGCCTTACC GTGGTCAGCA ACTCCTGTGG AACTCTATGG 
AACCTGTCAG CTCGCTCTGC CGAGGATCAG AAGTTCCTGT GGGACAATGG AGCAGTGCCC 
ATGCTCCGTT CGTTGATCCA CTCCAAACAC GCCATGATCT CAGAGGGCAG CTCTTCGGCC 
TTGAAGAACC TTTTGAACTT CCGACCAGCT GTTCAAAATC ATCACCAGCT GGATCCAATT 
GCCCGCTCCA TGGGCCTCAA AGCATTGCCC ACTCTGGAGG CACGAAAAGC CAAGGCATTG 
CAACAGGAGC TGGGTGAGCG TCACACGGCC GAGACGTGCG ATAACTTGGA CACGGGCGGA 
AAACTGGATA AAGAACGAGC CTCCAGTTCA TCCAGACGTC ATCCTGCGCC ACGCTTAACC 
CGATCGGCAA TGCTAACGAA GAGCGAGAGC AGGGATTCGG TGTACTCGGC CAAATCCGAT 
TGCGCTTACG ATCATCTAAT TCGCTCAGCC TCTGCTTCAG ATGCCCATCG AAAGGTGAAG 
CCCAAGATCA CGGACTTCGA TCTGGAAATG GAGCAGGACA CGGAGGCAAC CGAGGAACAA 
CCCATTGACT ACTCGGTGAA GTACAGCGAG AATGCCACCA AGACCTCAAC ATATCAGGAA 
ACGGACCTGG ATCAGCCCAC GGACTTCAGC CTTCGCTACG CTGAGAACCA AATTGAATCC 
GACCTGGACA TATCAGGACC AGCAGGCGGA CAAAAGAGCA CTATCACTCC TCCTGCTGAA 
ACAGTTCCCG AGAAATCCGA GGGCCAGGAA ATATTGTTGA TCCTAGACGA CAGTGTTAAG 
TGCTACCAGA CGGAGGATAC CCCTTATGTT ATATCCAATG CGGCTTCAGT CACAGATTTA 
CGAGTCGCTG CTAAAGCGGA TGCAGAAGCG GAAGTCAAGC CAGAAGTTCG CGAGGTGACT 
TCCAAAGAAG GAGCTCCCAA AAAGCTTCCC AAGCTTTCGC AGTGTGGCTC CGGTTCGTAC 
ACTCCAGAGA AACCCATTAA CTACTGCGAG GAGGGAACTC CTGGGTACTT TAGTCGCTAC 
GATTCACTGA GCAGCTTGGA TGAATCCGGT AAGGCTAACC AGGCAATAGT AGGAACGGAT 
GCGGATATCA AGCCCAAATT AGAGAAGCAG GAGGAACAGG AATCCCAGCC AGCAGAGCAG 
GTCCTGACCA AACCTCCCAC CCAGGCAAAC TCGGCATTGG AAACCCCGTT GATGTTCTCG 
CGACGCAGTT CCATGGACTC CCTAGTCCAC GATCCGGATG TCGATGTGGC CAACTGCGAT 
GACAAGAGCT CGGTGGTTAG TGACTTCAGT CGCTTGGCCA GTGGGGTAAT ATCCCCATCC 
GAGATCCCAG ATTCCCCCAC ACAAAGTATG CCCCAATCTC CACGACGAAA TAGTGTCGCT 
GGTTCGGGGC AGAATGTCGA TTCTCCACCG GTTGTAATCC CGGCCAGCCT ACAACCATTA 
CGCAGCGTTT TCGAGGACGA CCTGAGTAGC TTCAACGTGG AGCATACACC TGCTCAGTTC 
TCCACGGCCA CCAGTTTGAG CAACCTGAGC ATAGTGGACG ATGAAAAGGC ACCGGCCAGT 
GTGGCCGAGG AAGACAACGA AGATGAGCTC CTTTTGGCCA ACTGCATCAA CATGGGTATG 
CAGCGGAAGC CAACTGAGGC TGTGAAATCG ACAGTTGTGA ACTCAGAAGT GGACGTGGCT 
GAGGAGACCA TTCGTAGCTA CTGCACCGAA GATACGCCAG CATTGCTCTC CAAAGTACCA 
AGTAACACTA ACCTTTCGGT TATCTCCATG AGCTCCACCG ATCCCAAGGA TGCCACGGCT 
GGTCAGGCAC AGATGTACGC TCATCAGCTA TCCGACGATG TATCTTCCAA TGCATCGGAC 
TGCGGTGGAG CCTCTGGTCA CCTGCTACAG CAGTGCATCC GGGATGGCAT GAAGAAACCC 
CTCGGTGAAG CCACTTCAGA TCCTATAGCC ATGCTTCGAC GAGGCGGAAA CGAATTGCCT 
GGCTATCTGC CCTCTGCCGA TGAAATGAAC AAGTTTTTGG TGGAGGACAG TCCCTGTAAC 
TTCTCAGTGG TCTCTGGTTT ATCTAATCTC ACCGTGGGAT CCAGTCTGGT TGGGCCAGCC 
GTACAGCTCA AGGAGACCGA ACCATCCAGT GCGGATCAGA ATCCTGAGAT GAAGGCGAAG 
CCTGGCAAAC AGGAGCAAGT CCGAAGACCG CCGCATTGGC AAGATGATTC GCTGAGTTCT 
CTATCCATTG ACTCGGAAGA TGACACCAAC TTACTGAGTC AGGCTATTGC CGCTGGTTGC 
AATAGACCGA AATCGAATCT TGGCTTCAGC TCGAACGGCA AGCGCTCCAG CTCGCTGAGC 
TCCTCGCAGC CAATAGCCAT CAATGCGGCC ACCTCGGCCA GCTCCCTGAA TTCGGCGATG 
ACAGTACGCA AGAGCCAACA GCAGGAGTCC TACAGTTCCG TGGACTCTAG CGATTCTAAC 
GACAACCAGT CAAAGTCGCT CTTCGAGCTG TGCATCCTCA AGGGCATGTA CAAGACGAAG 
GAACCCGGCG CCCGGGCCCA GCAGATGCAG GAGCAACCTA TAGTGGGTTC ATCCTCCGTC 
CAGTCGAATC CCAGTCTAAA GCAGTTCGAT TCGCTGCCGG TGCAGCTGCC ATCTAGTGGA 
CAGGTCAAGC GGCAGCGCCA TCATCATCAT CACCATCATC ATAGGGAGCG AGAGCGAGAA 
CGCAAGGACG AGAAGCTGCT GCAGGAGTGC ATCAATACGG GCATCTCGAA GAAGATCAAC 
GCCGTGCCAA AAAACGTCCT GGCTACGTCT GCGGCTGCAT TGGAACCGTG TCACCCAATG 
GCAGCTACTA CATCAGCAAG TGCCCTCAGC ACAGCAGCTC CAGACGTAGA GCAGAAAGCG 
CACGCCACCA GCAATCCGCA GCAGCAGTCG TCCACGCACC CGAGCAGCCA CATCCTCCCG 
AATCCTATCG ACGCCATCGC CACCGTCACC GACACAGTAA GAAGTCCAGC AGCTCCAAAT 
CAAGGGAACG GGAACGCGAG TCAGAACGGT CTCGAGACCG CTACTGGGTC TAAGGACTTG 
GATTCGGAAG ATCGATCCAG CGATGAATCA AATCAGTCCT TCATCATGGA AACCATGGTC 
AGGTTGGACA GTGCTCTCAA CGAAACGTGC ATCTCCGGAG CAAGCGAGAA GCACAAGGAT 
CCCGACCTGA TGCTGAAGTC TGTGGAGAGA CTGACCATGG AGTTTGTTAC GTCGGCGGAG 
CAGTTGCGCA GCAGCAGCCA CAACCACAGC AGCAGCAACA GCCACAAGAA CAACAGTAGC 
AACAACACCT GGAACGAGAG CACCTGTCCC AATGATGTGA GTTTCCCCAG CGTCAGTCAA 
ACGGCACCGG TTCTGGCCTC CTTGAGCTTG GATGAAGATG CCACGGAGGC GAGATCCCTG 
CACGAGTTGA TTGAGATAAC GCCCACGAAT GAACAGCAAC CGGAATCGCT CGAAGGGGAA 
ACTGATACCC TGGTTAATGG TCATGCTGAT AGCTACTCAG GATCGTCTGG AGGTCTTAAC 
TTCCAGTTGG GTGGGCAGGT GCAGAATGCT GGCGTTAGAT TGGAGCCGCA AAGACTATTA 
TTTAATGGAA CTAGTGCCTC CATTATGACA AACTCGACCA TGATTGCGTT CGAAGCGCGA 
GCTCTGGCGG AAAATCTACT CCAGCCCGCT GCCACTGACG ATGACACCAC GGAGATGACC 
TTCAGCTTGA ACAGTTTGGA TCTGGATAAT ATTCGTCCGC CGTCGGGAAT GGAATCGCTG 
AACAGCTGCT ACCAGGATCA CTCCCAGCCT AGTTCTCTGC GCCAGGCGAT GCCCAGCAAG 
AGTCCACGCT TCGCGCGAAA AATGTTTCCA GCCAATTTGG TAGCCAGACG AGCACTGGGT 
CACTTAGCTG GCAGTGCGGA GAGCGTGAAC TCCAGCTGCA ACCTGCTGGA CAACATCAAG 
CCTCCGTCAC TGATGGATGA GCTGCTGGAC TCGATGATCA GTGTGGACAG CATTCAATCT 
GAGGTGGCCG ATGGCGAGCA GGACTGCAGC ATGGCCACCA CCATTTCCGT GTCCAACTAT 
GAGACGGCTG CTTGCGATGA TCAGACGATG ACCGTGCTCC AAAGTTGTTT CGACGAGGAT 
GAGGATGCCA CAATGAATGA CTACAGCTCG GCCGAGTCCA CACCCAAGCA CGGATCTACT 
CCATCACCCA ATCGTCGTTC CCTCACACCG AAAGACAAGC GACGTCTCAC AAAGGATCGC 
TTCAAGACCT ACACCATAGC CACCAGCTGC GAGATGGAAG CACCAGAGGC CAATGAAACT 
TTGCAGATAG AAATTGTGGA AGCTGCCGTT CCTGTGGCCA CGCCATCGCC ACGCGCCAAT 
GGAAGGAGAA GGGGCAGTGC GGAGCGGTAC AAGACCCAAC TGATTGAATG TCCCCTGGCT 
TTGATACAAC CCCAACCCGA TGATTGCCCA AGCGAACAGC TCTCCTCAAT TCGAGCAATG 
ATGCAACAGT TCACTTTCAT CACGGACATT AACATTGGAC ACAGTCAGGA GACTTGCGAG 
AGTACTGATC ATCCAGAAGA CGCAGGGGAA TCCCCGGAGT GCGATCAGAA CTCTGAGACG 
GAGTCGTGCG ATGGTCAGGA ACCGGATCAG CTGCCTCCTC CTCCGTCAAT TGTGGACCTT 
AGAACATCAG TCGTTAAGCC CACAACCTTG GAGCCCGCCA CGGCCGTAAA ACTGGTGCGA 
GGTCGCAAGA AACCAGCCTA TGTCTCACCC TACAGCATGC AAAGTCAACG GAATAGCAAC 
AATGCCGCCC CGTCCAAGAA GAAAACCCTA TCTCCAACAA TTGCCAAACG AAGTCTGGTG 
CCCGGCGGTT CAGGAGTTAG GTTACCAGCG AAAAAGAAGC CGACGCCGCC GCCTGAGCCG 
GCACCCGCAC GCTTGGAACG CCAGGGAACC TTTGTCAAGG ACGAGCCCAC TAACTCCAAT 
GTTCAAGTGC CCGTGGTGGA GACAAAACCG GCTCAAACCA GTCCCACTCA CCGCGCCTCG 
AAGTTACCCA CAAAGAAAGG CACGGCCAGC GGTGGGTCAC CATCGAAAGC GGGCTCTCCC 
AAGAGGATTC CCTTGGCGCC GGCCCGCCGA ATGACGCCTC AAAGGGCAAA CACCAGTCTG 
CGACTAGCTG CGGGCAAATC TCACGCTGCC TCACGTGTGG TCTCCGGCCG CGTGTCGTCC 
ACTACTCCAC CATCCCGCAG CAACTCCAAC CTAAATGGAA GCAGTGCAGC CGCAGCAGCT 
GCGGCCAAGA TCAACCATGC GCAAAGTCGT ATCGCCAACA TCTGGAAGAG AGTGGACGAG 
GCCAAAACCA AGCAATCGTC ATCCAACCTG AGGACGCAGA AAACCAAATC TTCCAACATG 
CTCAACGCAA ATGGAACCAA GCCGACGCTC CTGCGCAGCT CCACGTTTGA CAATACCCCC 
AGCACCGCTG GAGGAGTCAA GTCCAAGCTG CCAGTGGTGG GCGCGCGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Apc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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