MAPk-Ak2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAPk-Ak2
Entrez Gene ID	44573
Full Name	MAP kinase activated protein-kinase-2
Synonyms	CG3086,DmMAPKAPK-2,Dmel\CG3086,MAPKAP,MAPKAPK-2,MK2,dMK2
General protein information	Preferred Names MAP kinase activated protein-kinase-2 Names CG3086 gene product from transcript CG3086-RB CG3086-PB CG3086-PD CG3086-PE CG3086-PF MAP kinase activated protein kinase-2 MAPk-Ak2-PB MAPk-Ak2-PD MAPk-Ak2-PE MAPk-Ak2-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5B8-5B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_176688.3	NP_788861.1	MAP kinase activated protein-kinase-2, isoform D
NM_001201618.1	NP_001188547.1	MAP kinase activated protein-kinase-2, isoform E
NM_080030.5	NP_524769.1	MAP kinase activated protein-kinase-2, isoform B
NM_001258607.2	NP_001245536.1	MAP kinase activated protein-kinase-2, isoform F

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAPKAPK3	XP_516486.3
	MAPKAPK3	XP_001097318.1
Drosophila melanogaster (fruit fly)	MAPk-Ak2	NP_001188547.1
Mus musculus (house mouse)	Mapkapk3	NP_849238.1
Danio rerio (zebrafish)	mapkapk3	NP_001073548.1
Xenopus tropicalis (tropical clawed frog)	mapkapk3	NP_001072862.1
Bos taurus (cattle)	MAPKAPK3	NP_001029951.1
Gallus gallus (chicken)	MAPKAPK3	XP_414262.3
Canis lupus familiaris (dog)	MAPKAPK3	XP_541872.3
Rattus norvegicus (Norway rat)	Mapkapk3	NP_001012127.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011890	XP_320635.4
Caenorhabditis elegans (roundworm)	mak-2	NP_500005.1
Homo sapiens (human)	MAPKAPK3	NP_004626.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IscU is a new substrate of MK2 both in Drosophila cells and in human cells
Title: The p38 pathway regulates oxidative stress tolerance by phosphorylation of mitochondrial protein IscU.

The following MAPk-Ak2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAPk-Ak2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02350	NM_001258607.2 Latest version!	Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa02350	NM_080030.5 Latest version!	Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa02350	NM_176688.3 Latest version!	Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa02350	NM_001201618.1 Latest version!	Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAPk-Ak2 ( NM_176688.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788861.1 Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa02350
Clone ID Related Accession (Same CDS sequence)	NM_176688.3 , NM_001201618.1 , NM_080030.5 , NM_001258607.2
Accession Version	NM_176688.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	MAP kinase activated protein-kinase-2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_176688.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTTCTC TGCAGAATCA ACGGCAACCG AAGACCACCC CACTGACGGA CGACTACGTG 
ACCTCGAACA CGGTTCTGGG CTACGGGATC AACGGGAAGG TGGTGCAGTG CACCCATCGC 
CGCACCCAAC AGAACTATGC CCTCAAGGTG CTGTTGGACA GTGAAAGGGC GCGTCGCGAA 
GTGGACCTGC ATTGGCGGGT CAGCGGATGC CGGTACATTG TGAACATCAT TGATGTGTAC 
GAGAACACGT TTAAGGACAG GAAGTGCCTC CTGGTGGTCA TGGAGTGCAT GGAGGGCGGC 
GAGCTCTTCC AGAGAATTCA GGACAAGGCC GACGGTGCCT TTACGGAGCG GGAGGCGGCC 
CAGATAATGC ACGAGATCTG CGCGGCCGTA GACTATCTGC ACAGTCGCGA CATAGCGCAT 
CGCGATCTCA AGCCGGAGAA CTTGCTGTAC ACCACCACTC AGCCGAATGC GACCCTTAAA 
TTGACGGACT TTGGCTTCGC CAAGGAGACA TTCACCAGTT ACACGCTGCA GACGCCCTGC 
TACACTCCCT ATTACGTGGC TCCTGAGGTT CTGGGCCCGG AGAAGTACGA CAAGAGCTGC 
GACATCTGGT CGCTGGGCGT GGTGATGTAC ATCATTATGT GCGGATTCCC GCCGTTCTAC 
AGCAACCACG GCCTAGCCAT ATCGCCCGGC ATGAAAAATC GCATCCGCAC CGGCCAGTAT 
GACTTTCCCG ATCCGGAGTG GACGAATGTG AGCCAGGCGG CAAAGGATCT GATCAAGGGC 
ATGCTGAACG TGGACCCCAG CAAGCGTCTG CGCATCCAGG ACGTCATTAG CAACAAGTGG 
ATTGCCCAGT ACAATGCTGT TCCACAGACA CCGCTCTGCA CGGGTCGCAT GCTAAAGGAG 
GCCGAGGAAA CCTGGCCGGA AGTGCAGGAG GAGATGACGC GCTCACTGGC CACCATGCGC 
GTAGACTACG ATCAGATGCA AATCAAGGCG CTGGACAAGT CGAACAACCC GCTGCTGACG 
AAGCGCAGGA AAAAGATCGA GGAGATGGAG TTATACATGG CCAATGCGAC GCGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788861.1
CDS	158..1237
Translation	MLSLQNQRQPKTTPLTDDYVTSNTVLGYGINGKVVQCTHRRTQQNYALKVLLDSERARREVDLHWRVSGCRYIVNIIDVYENTFKDRKCLLVVMECMEGGELFQRIQDKADGAFTEREAAQIMHEICAAVDYLHSRDIAHRDLKPENLLYTTTQPNATLKLTDFGFAKETFTSYTLQTPCYTPYYVAPEVLGPEKYDKSCDIWSLGVVMYIIMCGFPPFYSNHGLAISPGMKNRIRTGQYDFPDPEWTNVSQAAKDLIKGMLNVDPSKRLRIQDVISNKWIAQYNAVPQTPLCTGRMLKEAEETWPEVQEEMTRSLATMRVDYDQMQIKALDKSNNPLLTKRRKKIEEMELYMANATRN

Target ORF information:

RefSeq Version	NM_176688.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176688.3

ORF Insert Sequence:

ATGCTTTCTC TGCAGAATCA ACGGCAACCG AAGACCACCC CACTGACGGA CGACTACGTG 
ACCTCGAACA CGGTTCTGGG CTACGGGATC AACGGGAAGG TGGTGCAGTG CACCCATCGC 
CGCACCCAAC AGAACTATGC CCTCAAGGTG CTGTTGGACA GTGAAAGGGC GCGTCGCGAA 
GTGGACCTGC ATTGGCGGGT CAGCGGATGC CGGTACATTG TGAACATCAT TGATGTGTAC 
GAGAACACGT TTAAGGACAG GAAGTGCCTC CTGGTGGTCA TGGAGTGCAT GGAGGGCGGC 
GAGCTCTTCC AGAGAATTCA GGACAAGGCC GACGGTGCCT TTACGGAGCG GGAGGCGGCC 
CAGATAATGC ACGAGATCTG CGCGGCCGTA GACTATCTGC ACAGTCGCGA CATAGCGCAT 
CGCGATCTCA AGCCGGAGAA CTTGCTGTAC ACCACCACTC AGCCGAATGC GACCCTTAAA 
TTGACGGACT TTGGCTTCGC CAAGGAGACA TTCACCAGTT ACACGCTGCA GACGCCCTGC 
TACACTCCCT ATTACGTGGC TCCTGAGGTT CTGGGCCCGG AGAAGTACGA CAAGAGCTGC 
GACATCTGGT CGCTGGGCGT GGTGATGTAC ATCATTATGT GCGGATTCCC GCCGTTCTAC 
AGCAACCACG GCCTAGCCAT ATCGCCCGGC ATGAAAAATC GCATCCGCAC CGGCCAGTAT 
GACTTTCCCG ATCCGGAGTG GACGAATGTG AGCCAGGCGG CAAAGGATCT GATCAAGGGC 
ATGCTGAACG TGGACCCCAG CAAGCGTCTG CGCATCCAGG ACGTCATTAG CAACAAGTGG 
ATTGCCCAGT ACAATGCTGT TCCACAGACA CCGCTCTGCA CGGGTCGCAT GCTAAAGGAG 
GCCGAGGAAA CCTGGCCGGA AGTGCAGGAG GAGATGACGC GCTCACTGGC CACCATGCGC 
GTAGACTACG ATCAGATGCA AATCAAGGCG CTGGACAAGT CGAACAACCC GCTGCTGACG 
AAGCGCAGGA AAAAGATCGA GGAGATGGAG TTATACATGG CCAATGCGAC GCGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPk-Ak2 ( NM_001201618.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188547.1 Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa02350
Clone ID Related Accession (Same CDS sequence)	NM_176688.3 , NM_001201618.1 , NM_080030.5 , NM_001258607.2
Accession Version	NM_001201618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	MAP kinase activated protein-kinase-2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTTCTC TGCAGAATCA ACGGCAACCG AAGACCACCC CACTGACGGA CGACTACGTG 
ACCTCGAACA CGGTTCTGGG CTACGGGATC AACGGGAAGG TGGTGCAGTG CACCCATCGC 
CGCACCCAAC AGAACTATGC CCTCAAGGTG CTGTTGGACA GTGAAAGGGC GCGTCGCGAA 
GTGGACCTGC ATTGGCGGGT CAGCGGATGC CGGTACATTG TGAACATCAT TGATGTGTAC 
GAGAACACGT TTAAGGACAG GAAGTGCCTC CTGGTGGTCA TGGAGTGCAT GGAGGGCGGC 
GAGCTCTTCC AGAGAATTCA GGACAAGGCC GACGGTGCCT TTACGGAGCG GGAGGCGGCC 
CAGATAATGC ACGAGATCTG CGCGGCCGTA GACTATCTGC ACAGTCGCGA CATAGCGCAT 
CGCGATCTCA AGCCGGAGAA CTTGCTGTAC ACCACCACTC AGCCGAATGC GACCCTTAAA 
TTGACGGACT TTGGCTTCGC CAAGGAGACA TTCACCAGTT ACACGCTGCA GACGCCCTGC 
TACACTCCCT ATTACGTGGC TCCTGAGGTT CTGGGCCCGG AGAAGTACGA CAAGAGCTGC 
GACATCTGGT CGCTGGGCGT GGTGATGTAC ATCATTATGT GCGGATTCCC GCCGTTCTAC 
AGCAACCACG GCCTAGCCAT ATCGCCCGGC ATGAAAAATC GCATCCGCAC CGGCCAGTAT 
GACTTTCCCG ATCCGGAGTG GACGAATGTG AGCCAGGCGG CAAAGGATCT GATCAAGGGC 
ATGCTGAACG TGGACCCCAG CAAGCGTCTG CGCATCCAGG ACGTCATTAG CAACAAGTGG 
ATTGCCCAGT ACAATGCTGT TCCACAGACA CCGCTCTGCA CGGGTCGCAT GCTAAAGGAG 
GCCGAGGAAA CCTGGCCGGA AGTGCAGGAG GAGATGACGC GCTCACTGGC CACCATGCGC 
GTAGACTACG ATCAGATGCA AATCAAGGCG CTGGACAAGT CGAACAACCC GCTGCTGACG 
AAGCGCAGGA AAAAGATCGA GGAGATGGAG TTATACATGG CCAATGCGAC GCGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188547.1
CDS	173..1252
Translation	MLSLQNQRQPKTTPLTDDYVTSNTVLGYGINGKVVQCTHRRTQQNYALKVLLDSERARREVDLHWRVSGCRYIVNIIDVYENTFKDRKCLLVVMECMEGGELFQRIQDKADGAFTEREAAQIMHEICAAVDYLHSRDIAHRDLKPENLLYTTTQPNATLKLTDFGFAKETFTSYTLQTPCYTPYYVAPEVLGPEKYDKSCDIWSLGVVMYIIMCGFPPFYSNHGLAISPGMKNRIRTGQYDFPDPEWTNVSQAAKDLIKGMLNVDPSKRLRIQDVISNKWIAQYNAVPQTPLCTGRMLKEAEETWPEVQEEMTRSLATMRVDYDQMQIKALDKSNNPLLTKRRKKIEEMELYMANATRN

Target ORF information:

RefSeq Version	NM_001201618.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201618.1

ORF Insert Sequence:

ATGCTTTCTC TGCAGAATCA ACGGCAACCG AAGACCACCC CACTGACGGA CGACTACGTG 
ACCTCGAACA CGGTTCTGGG CTACGGGATC AACGGGAAGG TGGTGCAGTG CACCCATCGC 
CGCACCCAAC AGAACTATGC CCTCAAGGTG CTGTTGGACA GTGAAAGGGC GCGTCGCGAA 
GTGGACCTGC ATTGGCGGGT CAGCGGATGC CGGTACATTG TGAACATCAT TGATGTGTAC 
GAGAACACGT TTAAGGACAG GAAGTGCCTC CTGGTGGTCA TGGAGTGCAT GGAGGGCGGC 
GAGCTCTTCC AGAGAATTCA GGACAAGGCC GACGGTGCCT TTACGGAGCG GGAGGCGGCC 
CAGATAATGC ACGAGATCTG CGCGGCCGTA GACTATCTGC ACAGTCGCGA CATAGCGCAT 
CGCGATCTCA AGCCGGAGAA CTTGCTGTAC ACCACCACTC AGCCGAATGC GACCCTTAAA 
TTGACGGACT TTGGCTTCGC CAAGGAGACA TTCACCAGTT ACACGCTGCA GACGCCCTGC 
TACACTCCCT ATTACGTGGC TCCTGAGGTT CTGGGCCCGG AGAAGTACGA CAAGAGCTGC 
GACATCTGGT CGCTGGGCGT GGTGATGTAC ATCATTATGT GCGGATTCCC GCCGTTCTAC 
AGCAACCACG GCCTAGCCAT ATCGCCCGGC ATGAAAAATC GCATCCGCAC CGGCCAGTAT 
GACTTTCCCG ATCCGGAGTG GACGAATGTG AGCCAGGCGG CAAAGGATCT GATCAAGGGC 
ATGCTGAACG TGGACCCCAG CAAGCGTCTG CGCATCCAGG ACGTCATTAG CAACAAGTGG 
ATTGCCCAGT ACAATGCTGT TCCACAGACA CCGCTCTGCA CGGGTCGCAT GCTAAAGGAG 
GCCGAGGAAA CCTGGCCGGA AGTGCAGGAG GAGATGACGC GCTCACTGGC CACCATGCGC 
GTAGACTACG ATCAGATGCA AATCAAGGCG CTGGACAAGT CGAACAACCC GCTGCTGACG 
AAGCGCAGGA AAAAGATCGA GGAGATGGAG TTATACATGG CCAATGCGAC GCGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPk-Ak2 ( NM_080030.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524769.1 Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02350
Clone ID Related Accession (Same CDS sequence)	NM_176688.3 , NM_001201618.1 , NM_080030.5 , NM_001258607.2
Accession Version	NM_080030.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	MAP kinase activated protein-kinase-2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080030.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTTCTC TGCAGAATCA ACGGCAACCG AAGACCACCC CACTGACGGA CGACTACGTG 
ACCTCGAACA CGGTTCTGGG CTACGGGATC AACGGGAAGG TGGTGCAGTG CACCCATCGC 
CGCACCCAAC AGAACTATGC CCTCAAGGTG CTGTTGGACA GTGAAAGGGC GCGTCGCGAA 
GTGGACCTGC ATTGGCGGGT CAGCGGATGC CGGTACATTG TGAACATCAT TGATGTGTAC 
GAGAACACGT TTAAGGACAG GAAGTGCCTC CTGGTGGTCA TGGAGTGCAT GGAGGGCGGC 
GAGCTCTTCC AGAGAATTCA GGACAAGGCC GACGGTGCCT TTACGGAGCG GGAGGCGGCC 
CAGATAATGC ACGAGATCTG CGCGGCCGTA GACTATCTGC ACAGTCGCGA CATAGCGCAT 
CGCGATCTCA AGCCGGAGAA CTTGCTGTAC ACCACCACTC AGCCGAATGC GACCCTTAAA 
TTGACGGACT TTGGCTTCGC CAAGGAGACA TTCACCAGTT ACACGCTGCA GACGCCCTGC 
TACACTCCCT ATTACGTGGC TCCTGAGGTT CTGGGCCCGG AGAAGTACGA CAAGAGCTGC 
GACATCTGGT CGCTGGGCGT GGTGATGTAC ATCATTATGT GCGGATTCCC GCCGTTCTAC 
AGCAACCACG GCCTAGCCAT ATCGCCCGGC ATGAAAAATC GCATCCGCAC CGGCCAGTAT 
GACTTTCCCG ATCCGGAGTG GACGAATGTG AGCCAGGCGG CAAAGGATCT GATCAAGGGC 
ATGCTGAACG TGGACCCCAG CAAGCGTCTG CGCATCCAGG ACGTCATTAG CAACAAGTGG 
ATTGCCCAGT ACAATGCTGT TCCACAGACA CCGCTCTGCA CGGGTCGCAT GCTAAAGGAG 
GCCGAGGAAA CCTGGCCGGA AGTGCAGGAG GAGATGACGC GCTCACTGGC CACCATGCGC 
GTAGACTACG ATCAGATGCA AATCAAGGCG CTGGACAAGT CGAACAACCC GCTGCTGACG 
AAGCGCAGGA AAAAGATCGA GGAGATGGAG TTATACATGG CCAATGCGAC GCGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524769.1
CDS	133..1212
Translation	MLSLQNQRQPKTTPLTDDYVTSNTVLGYGINGKVVQCTHRRTQQNYALKVLLDSERARREVDLHWRVSGCRYIVNIIDVYENTFKDRKCLLVVMECMEGGELFQRIQDKADGAFTEREAAQIMHEICAAVDYLHSRDIAHRDLKPENLLYTTTQPNATLKLTDFGFAKETFTSYTLQTPCYTPYYVAPEVLGPEKYDKSCDIWSLGVVMYIIMCGFPPFYSNHGLAISPGMKNRIRTGQYDFPDPEWTNVSQAAKDLIKGMLNVDPSKRLRIQDVISNKWIAQYNAVPQTPLCTGRMLKEAEETWPEVQEEMTRSLATMRVDYDQMQIKALDKSNNPLLTKRRKKIEEMELYMANATRN

Target ORF information:

RefSeq Version	NM_080030.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080030.5

ORF Insert Sequence:

ATGCTTTCTC TGCAGAATCA ACGGCAACCG AAGACCACCC CACTGACGGA CGACTACGTG 
ACCTCGAACA CGGTTCTGGG CTACGGGATC AACGGGAAGG TGGTGCAGTG CACCCATCGC 
CGCACCCAAC AGAACTATGC CCTCAAGGTG CTGTTGGACA GTGAAAGGGC GCGTCGCGAA 
GTGGACCTGC ATTGGCGGGT CAGCGGATGC CGGTACATTG TGAACATCAT TGATGTGTAC 
GAGAACACGT TTAAGGACAG GAAGTGCCTC CTGGTGGTCA TGGAGTGCAT GGAGGGCGGC 
GAGCTCTTCC AGAGAATTCA GGACAAGGCC GACGGTGCCT TTACGGAGCG GGAGGCGGCC 
CAGATAATGC ACGAGATCTG CGCGGCCGTA GACTATCTGC ACAGTCGCGA CATAGCGCAT 
CGCGATCTCA AGCCGGAGAA CTTGCTGTAC ACCACCACTC AGCCGAATGC GACCCTTAAA 
TTGACGGACT TTGGCTTCGC CAAGGAGACA TTCACCAGTT ACACGCTGCA GACGCCCTGC 
TACACTCCCT ATTACGTGGC TCCTGAGGTT CTGGGCCCGG AGAAGTACGA CAAGAGCTGC 
GACATCTGGT CGCTGGGCGT GGTGATGTAC ATCATTATGT GCGGATTCCC GCCGTTCTAC 
AGCAACCACG GCCTAGCCAT ATCGCCCGGC ATGAAAAATC GCATCCGCAC CGGCCAGTAT 
GACTTTCCCG ATCCGGAGTG GACGAATGTG AGCCAGGCGG CAAAGGATCT GATCAAGGGC 
ATGCTGAACG TGGACCCCAG CAAGCGTCTG CGCATCCAGG ACGTCATTAG CAACAAGTGG 
ATTGCCCAGT ACAATGCTGT TCCACAGACA CCGCTCTGCA CGGGTCGCAT GCTAAAGGAG 
GCCGAGGAAA CCTGGCCGGA AGTGCAGGAG GAGATGACGC GCTCACTGGC CACCATGCGC 
GTAGACTACG ATCAGATGCA AATCAAGGCG CTGGACAAGT CGAACAACCC GCTGCTGACG 
AAGCGCAGGA AAAAGATCGA GGAGATGGAG TTATACATGG CCAATGCGAC GCGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPk-Ak2 ( NM_001258607.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245536.1 Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa02350
Clone ID Related Accession (Same CDS sequence)	NM_176688.3 , NM_001201618.1 , NM_080030.5 , NM_001258607.2
Accession Version	NM_001258607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	MAP kinase activated protein-kinase-2, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258607.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTTCTC TGCAGAATCA ACGGCAACCG AAGACCACCC CACTGACGGA CGACTACGTG 
ACCTCGAACA CGGTTCTGGG CTACGGGATC AACGGGAAGG TGGTGCAGTG CACCCATCGC 
CGCACCCAAC AGAACTATGC CCTCAAGGTG CTGTTGGACA GTGAAAGGGC GCGTCGCGAA 
GTGGACCTGC ATTGGCGGGT CAGCGGATGC CGGTACATTG TGAACATCAT TGATGTGTAC 
GAGAACACGT TTAAGGACAG GAAGTGCCTC CTGGTGGTCA TGGAGTGCAT GGAGGGCGGC 
GAGCTCTTCC AGAGAATTCA GGACAAGGCC GACGGTGCCT TTACGGAGCG GGAGGCGGCC 
CAGATAATGC ACGAGATCTG CGCGGCCGTA GACTATCTGC ACAGTCGCGA CATAGCGCAT 
CGCGATCTCA AGCCGGAGAA CTTGCTGTAC ACCACCACTC AGCCGAATGC GACCCTTAAA 
TTGACGGACT TTGGCTTCGC CAAGGAGACA TTCACCAGTT ACACGCTGCA GACGCCCTGC 
TACACTCCCT ATTACGTGGC TCCTGAGGTT CTGGGCCCGG AGAAGTACGA CAAGAGCTGC 
GACATCTGGT CGCTGGGCGT GGTGATGTAC ATCATTATGT GCGGATTCCC GCCGTTCTAC 
AGCAACCACG GCCTAGCCAT ATCGCCCGGC ATGAAAAATC GCATCCGCAC CGGCCAGTAT 
GACTTTCCCG ATCCGGAGTG GACGAATGTG AGCCAGGCGG CAAAGGATCT GATCAAGGGC 
ATGCTGAACG TGGACCCCAG CAAGCGTCTG CGCATCCAGG ACGTCATTAG CAACAAGTGG 
ATTGCCCAGT ACAATGCTGT TCCACAGACA CCGCTCTGCA CGGGTCGCAT GCTAAAGGAG 
GCCGAGGAAA CCTGGCCGGA AGTGCAGGAG GAGATGACGC GCTCACTGGC CACCATGCGC 
GTAGACTACG ATCAGATGCA AATCAAGGCG CTGGACAAGT CGAACAACCC GCTGCTGACG 
AAGCGCAGGA AAAAGATCGA GGAGATGGAG TTATACATGG CCAATGCGAC GCGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245536.1
CDS	26..1105
Translation	MLSLQNQRQPKTTPLTDDYVTSNTVLGYGINGKVVQCTHRRTQQNYALKVLLDSERARREVDLHWRVSGCRYIVNIIDVYENTFKDRKCLLVVMECMEGGELFQRIQDKADGAFTEREAAQIMHEICAAVDYLHSRDIAHRDLKPENLLYTTTQPNATLKLTDFGFAKETFTSYTLQTPCYTPYYVAPEVLGPEKYDKSCDIWSLGVVMYIIMCGFPPFYSNHGLAISPGMKNRIRTGQYDFPDPEWTNVSQAAKDLIKGMLNVDPSKRLRIQDVISNKWIAQYNAVPQTPLCTGRMLKEAEETWPEVQEEMTRSLATMRVDYDQMQIKALDKSNNPLLTKRRKKIEEMELYMANATRN

Target ORF information:

RefSeq Version	NM_001258607.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MAP kinase activated protein-kinase-2 (MAPk-Ak2), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258607.2

ORF Insert Sequence:

ATGCTTTCTC TGCAGAATCA ACGGCAACCG AAGACCACCC CACTGACGGA CGACTACGTG 
ACCTCGAACA CGGTTCTGGG CTACGGGATC AACGGGAAGG TGGTGCAGTG CACCCATCGC 
CGCACCCAAC AGAACTATGC CCTCAAGGTG CTGTTGGACA GTGAAAGGGC GCGTCGCGAA 
GTGGACCTGC ATTGGCGGGT CAGCGGATGC CGGTACATTG TGAACATCAT TGATGTGTAC 
GAGAACACGT TTAAGGACAG GAAGTGCCTC CTGGTGGTCA TGGAGTGCAT GGAGGGCGGC 
GAGCTCTTCC AGAGAATTCA GGACAAGGCC GACGGTGCCT TTACGGAGCG GGAGGCGGCC 
CAGATAATGC ACGAGATCTG CGCGGCCGTA GACTATCTGC ACAGTCGCGA CATAGCGCAT 
CGCGATCTCA AGCCGGAGAA CTTGCTGTAC ACCACCACTC AGCCGAATGC GACCCTTAAA 
TTGACGGACT TTGGCTTCGC CAAGGAGACA TTCACCAGTT ACACGCTGCA GACGCCCTGC 
TACACTCCCT ATTACGTGGC TCCTGAGGTT CTGGGCCCGG AGAAGTACGA CAAGAGCTGC 
GACATCTGGT CGCTGGGCGT GGTGATGTAC ATCATTATGT GCGGATTCCC GCCGTTCTAC 
AGCAACCACG GCCTAGCCAT ATCGCCCGGC ATGAAAAATC GCATCCGCAC CGGCCAGTAT 
GACTTTCCCG ATCCGGAGTG GACGAATGTG AGCCAGGCGG CAAAGGATCT GATCAAGGGC 
ATGCTGAACG TGGACCCCAG CAAGCGTCTG CGCATCCAGG ACGTCATTAG CAACAAGTGG 
ATTGCCCAGT ACAATGCTGT TCCACAGACA CCGCTCTGCA CGGGTCGCAT GCTAAAGGAG 
GCCGAGGAAA CCTGGCCGGA AGTGCAGGAG GAGATGACGC GCTCACTGGC CACCATGCGC 
GTAGACTACG ATCAGATGCA AATCAAGGCG CTGGACAAGT CGAACAACCC GCTGCTGACG 
AAGCGCAGGA AAAAGATCGA GGAGATGGAG TTATACATGG CCAATGCGAC GCGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MAPk-Ak2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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