PPO3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPO3
Entrez Gene ID	44513
Full Name	Prophenoloxidase 3
Synonyms	CG2952,CG42640,DmePPO3,Dmel\CG42640,Dmel_CG2952,Dox-A3,Pro-PO3,ProPO59,proPO59
General protein information	Preferred Names Prophenoloxidase 3 Names CG42640 gene product from transcript CG42640-RA CG42640-PA PPO3-PA diphenol OxidaseA3 prophenoloxidase 3 prophenoloxidase 59
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 59C3-59C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_080021.4	NP_524760.1	prophenoloxidase 3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PPO1 and PPO2 contribute to the bulk of melanization in the hemolymph, upon septic or clean injury. In contrast, PPO3, is expressed in lamellocytes and contributes to melanization during the encapsulation process.
Title: Drosophila innate immunity: regional and functional specialization of prophenoloxidases.

Study found that some amino acids are important for rPPO3 (CG2952) (Cu(2+)-aided auto-activation as well as PPO staining activity in vitro.
Title: Specific amino acids affecting Drosophila melanogaster prophenoloxidase activity in vitro.

rPPO1 and rPPO2 were expressed as apo-rPPO and became holo-PPO upon Cu(2+) binding; rPPO3 was holo-PPO immediately after expression.
Title: Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro.

These results indicate that the expression of CG2952 is very low and that CG8193 is the gene that encodes proPO A3.
Title: Identification of the gene encoding pro-phenoloxidase A(3) in the fruitfly, Drosophila melanogaster.

biochemical properties, activation mode and in vivo role of Pro-PO3 (PPO3) are likely distinct from those of the other two Pro-PO enzymes involved in Drosophila physiology.
Title: Involvement of pro-phenoloxidase 3 in lamellocyte-mediated spontaneous melanization in Drosophila.

The following PPO3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPO3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11348	NM_080021.4 Latest version!	Drosophila melanogaster prophenoloxidase 3 (PPO3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPO3 ( NM_080021.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524760.1 Drosophila melanogaster prophenoloxidase 3 (PPO3), mRNA.

Price & Availability↑

CloneID	OFa11348
Clone ID Related Accession (Same CDS sequence)	NM_080021.4
Accession Version	NM_080021.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	prophenoloxidase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_080021.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACA AGAAGAATCT GCTCCTTCTG TTCGATCATC CCACGGAGCC CGTGTTCATG 
GACAAGGGTG GCAATGGCAC CGTCTTCGAC GTGCCCGACT CCTACGTGAC CGATCGCTAC 
AATCAGATGT GCAAGAAGGT CCAGCGGCGC GTGAGCAGCG CGTCCGAGAA GAACGTTCAA 
GTCAAGGAGA TCGCCATACC GGATCTCAGT TGCTCCATGA GACTGGGCCG CTCGGAGCAG 
TTCTCGATCT TCCTGAAGTC CCACCGCAAG ATGGCCAGCC ACCTGATCGA GATCTTCACC 
AAAATGCAGA CCGTGGATGA GCTGCAGAGC GTGGCGGTGT ACGCCAGGGA TCGCGTCAAT 
CCGGTGCTCT TCAACTACGC CTTGTCGGTG GCCCTGCTCC ACCGTCCGGA CACCAAGGAC 
CTCGAACTGC CCGCCTTTGC GCAGACCTTC CCGGATCGCT TTATCGACTC GAAGATGCTT 
CGCAGCATGC GCGAGGAGTC CTTTGTGGTG GAGCGCTCCG CCGCCCGCCT GCCCGTCGTC 
AGTTCGGTCA AGTACACCGC CTCCGATCTC GATGTGGAGC ACCGGCTGTG GTACTTCCGC 
GAGGACCTGG GCGTCAACCT GCACCACTGG CACTGGCATC TGGTCTATCC CATAGAGGCG 
CCCGACCGCA GCATCGTGGA CAAGGACCGT CGTGGCGAGC TGTTCTACTA TATGCACCAG 
CAGATCATTG CCCGCTACAA TGCCGAGCGG CTGAGCAACC ACATGGCCCG GGTGCAGCCG 
TTCAACAACC TGGACGAACC AATTGCCGAG GGCTACTTCC CCAAGATGGA CTCGCTGGTG 
GCCAGTAGGG CGTATCCTCC GCGCTTCGAT AACACCCGGC TGAGCGATGT GGACCGGCCT 
AACAACCAGT TGAGGGTTGG CATCGATGAT ATGAAGCGCT GGCGCGAACG CATCTACGAG 
GCCATCCATC AGGGCTACGT TTTGGATACG AACAATGAAA AGATCGTGCT GGATGACGCT 
AAGGGCATCG ACATCCTGGG CAACATCATC GAGGCTTCCG ATCTGACGCC CAACAGTACG 
CTGTACGGCG ACTTCCACAA CATGGGGCAC ATACTGATTG CCTACTCACA CGATCCGACC 
AACAAGCACC TGGAGTACGC GGGCGTGATG GGCGACGCCT CCACCGCGAT GCGCGATCCG 
ATCTTCTACA AGTGGCACGC CTTCATCGAC AATATGTTCC AGGAGCACAA ACGTCTGCTT 
TCTCCCTATG AGAAGCAAGA GCTCAGCTTT CCGGACGTTC GCGTCGAGAG CATCCAGGTG 
GAGAGCCAGG GTCAGGTCAA CAGGCTGACC ACCTTCTGGC AGGAGTCCGA TGTGGACATG 
TCCCGTGGCC TTGATTTTGT GCCGCGCGGC CATGTCCTCG CTCGGTTCAC CCATCTGCAG 
CACCACGAAT TCAGCTACAC CATCAAGGTG GAGAACTCCA GCGAGGCCAC GCGGTATGGC 
TACGTGCGCA TCTTCCTGGC GCCCAAGCTG GATGATCGCA ATGCACCCAT GCTGCTGGAG 
CAACAGCGCC TGATGATGGT GGAGCTGGAC AAGTTCGTGG TTACCATGCC TCCCGGCAGC 
CATACGATCA CCCGGAACTC CACGGAGTCG AGTGTCACCA TTCCGTTCGA GCGCACCTTC 
CGCAACCTGG ACAAGCTGGA GGAGCTGCAG AACTTCTTGT GCGGCTGCGG TTGGCCCCAG 
CACATGCTGA TTCCGAAGGG TCGGCCCGAG GGTCTGCGCT TCGAGCTCTT CGTCATGGTT 
TCCAACTACG AAGAGGATAA GGTTGACCAG ACGGTCGCAG ATTGCGGCTG CAGCATCGCC 
GCTTCCTACT GCGGCCTGCG CGATCGCCTC TATCCGGATC GCAAGTCCAT GGGCTTCCCC 
TTCGATCGCA AGCCGCGTCG AGGCTCCGAG ATCCTGGAGA ACTTCCTGAC GCCCAACATG 
TGCGCCGTGG AGGTCATCAT TACCCACGAG GACCGCACCG AGAAGCTTCG CGAGGTACCA 
GCTCGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524760.1
CDS	61..2112
Translation	MADKKNLLLLFDHPTEPVFMDKGGNGTVFDVPDSYVTDRYNQMCKKVQRRVSSASEKNVQVKEIAIPDLSCSMRLGRSEQFSIFLKSHRKMASHLIEIFTKMQTVDELQSVAVYARDRVNPVLFNYALSVALLHRPDTKDLELPAFAQTFPDRFIDSKMLRSMREESFVVERSAARLPVVSSVKYTASDLDVEHRLWYFREDLGVNLHHWHWHLVYPIEAPDRSIVDKDRRGELFYYMHQQIIARYNAERLSNHMARVQPFNNLDEPIAEGYFPKMDSLVASRAYPPRFDNTRLSDVDRPNNQLRVGIDDMKRWRERIYEAIHQGYVLDTNNEKIVLDDAKGIDILGNIIEASDLTPNSTLYGDFHNMGHILIAYSHDPTNKHLEYAGVMGDASTAMRDPIFYKWHAFIDNMFQEHKRLLSPYEKQELSFPDVRVESIQVESQGQVNRLTTFWQESDVDMSRGLDFVPRGHVLARFTHLQHHEFSYTIKVENSSEATRYGYVRIFLAPKLDDRNAPMLLEQQRLMMVELDKFVVTMPPGSHTITRNSTESSVTIPFERTFRNLDKLEELQNFLCGCGWPQHMLIPKGRPEGLRFELFVMVSNYEEDKVDQTVADCGCSIAASYCGLRDRLYPDRKSMGFPFDRKPRRGSEILENFLTPNMCAVEVIITHEDRTEKLREVPARS

Target ORF information:

RefSeq Version	NM_080021.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster prophenoloxidase 3 (PPO3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080021.4

ORF Insert Sequence:

ATGGCCGACA AGAAGAATCT GCTCCTTCTG TTCGATCATC CCACGGAGCC CGTGTTCATG 
GACAAGGGTG GCAATGGCAC CGTCTTCGAC GTGCCCGACT CCTACGTGAC CGATCGCTAC 
AATCAGATGT GCAAGAAGGT CCAGCGGCGC GTGAGCAGCG CGTCCGAGAA GAACGTTCAA 
GTCAAGGAGA TCGCCATACC GGATCTCAGT TGCTCCATGA GACTGGGCCG CTCGGAGCAG 
TTCTCGATCT TCCTGAAGTC CCACCGCAAG ATGGCCAGCC ACCTGATCGA GATCTTCACC 
AAAATGCAGA CCGTGGATGA GCTGCAGAGC GTGGCGGTGT ACGCCAGGGA TCGCGTCAAT 
CCGGTGCTCT TCAACTACGC CTTGTCGGTG GCCCTGCTCC ACCGTCCGGA CACCAAGGAC 
CTCGAACTGC CCGCCTTTGC GCAGACCTTC CCGGATCGCT TTATCGACTC GAAGATGCTT 
CGCAGCATGC GCGAGGAGTC CTTTGTGGTG GAGCGCTCCG CCGCCCGCCT GCCCGTCGTC 
AGTTCGGTCA AGTACACCGC CTCCGATCTC GATGTGGAGC ACCGGCTGTG GTACTTCCGC 
GAGGACCTGG GCGTCAACCT GCACCACTGG CACTGGCATC TGGTCTATCC CATAGAGGCG 
CCCGACCGCA GCATCGTGGA CAAGGACCGT CGTGGCGAGC TGTTCTACTA TATGCACCAG 
CAGATCATTG CCCGCTACAA TGCCGAGCGG CTGAGCAACC ACATGGCCCG GGTGCAGCCG 
TTCAACAACC TGGACGAACC AATTGCCGAG GGCTACTTCC CCAAGATGGA CTCGCTGGTG 
GCCAGTAGGG CGTATCCTCC GCGCTTCGAT AACACCCGGC TGAGCGATGT GGACCGGCCT 
AACAACCAGT TGAGGGTTGG CATCGATGAT ATGAAGCGCT GGCGCGAACG CATCTACGAG 
GCCATCCATC AGGGCTACGT TTTGGATACG AACAATGAAA AGATCGTGCT GGATGACGCT 
AAGGGCATCG ACATCCTGGG CAACATCATC GAGGCTTCCG ATCTGACGCC CAACAGTACG 
CTGTACGGCG ACTTCCACAA CATGGGGCAC ATACTGATTG CCTACTCACA CGATCCGACC 
AACAAGCACC TGGAGTACGC GGGCGTGATG GGCGACGCCT CCACCGCGAT GCGCGATCCG 
ATCTTCTACA AGTGGCACGC CTTCATCGAC AATATGTTCC AGGAGCACAA ACGTCTGCTT 
TCTCCCTATG AGAAGCAAGA GCTCAGCTTT CCGGACGTTC GCGTCGAGAG CATCCAGGTG 
GAGAGCCAGG GTCAGGTCAA CAGGCTGACC ACCTTCTGGC AGGAGTCCGA TGTGGACATG 
TCCCGTGGCC TTGATTTTGT GCCGCGCGGC CATGTCCTCG CTCGGTTCAC CCATCTGCAG 
CACCACGAAT TCAGCTACAC CATCAAGGTG GAGAACTCCA GCGAGGCCAC GCGGTATGGC 
TACGTGCGCA TCTTCCTGGC GCCCAAGCTG GATGATCGCA ATGCACCCAT GCTGCTGGAG 
CAACAGCGCC TGATGATGGT GGAGCTGGAC AAGTTCGTGG TTACCATGCC TCCCGGCAGC 
CATACGATCA CCCGGAACTC CACGGAGTCG AGTGTCACCA TTCCGTTCGA GCGCACCTTC 
CGCAACCTGG ACAAGCTGGA GGAGCTGCAG AACTTCTTGT GCGGCTGCGG TTGGCCCCAG 
CACATGCTGA TTCCGAAGGG TCGGCCCGAG GGTCTGCGCT TCGAGCTCTT CGTCATGGTT 
TCCAACTACG AAGAGGATAA GGTTGACCAG ACGGTCGCAG ATTGCGGCTG CAGCATCGCC 
GCTTCCTACT GCGGCCTGCG CGATCGCCTC TATCCGGATC GCAAGTCCAT GGGCTTCCCC 
TTCGATCGCA AGCCGCGTCG AGGCTCCGAG ATCCTGGAGA ACTTCCTGAC GCCCAACATG 
TGCGCCGTGG AGGTCATCAT TACCCACGAG GACCGCACCG AGAAGCTTCG CGAGGTACCA 
GCTCGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PPO3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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