CanB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CanB
Entrez Gene ID	44317
Full Name	Calcineurin B
Synonyms	BcDNA:RH11383,CG4209,CNB,CaNB,Can,CnB,Dmel\CG4209,canB-4F,dCnB1
General protein information	Preferred Names Calcineurin B Names CG4209 gene product from transcript CG4209-RB CG4209-PA CG4209-PB Calcineurin1 CanB-PA CanB-PB calcineurin B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4F5-4F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_080002.4	NP_524741.1	calcineurin B, isoform A
NM_001258602.1	NP_001245531.1	calcineurin B, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP3R1	XP_855383.3
Magnaporthe oryzae (rice blast fungus)	MGG_06933	XP_003709672.1
Neurospora crassa	NCU03833	XP_960592.2
Pan troglodytes (chimpanzee)	PPP3R1	XP_003309091.1
Mus musculus (house mouse)	Ppp3r1	NP_077779.2
Rattus norvegicus (Norway rat)	Ppp3r1	NP_059005.1
Eremothecium gossypii	AGOS_AER096C	NP_984956.1
Schizosaccharomyces pombe (fission yeast)	SPCC830.06	NP_588476.1
	LOC100428682	XP_002799342.1
Bos taurus (cattle)	PPP3R1	NP_777008.1
Gallus gallus (chicken)	PPP3R1	NP_989707.1
Drosophila melanogaster (fruit fly)	CanB	NP_524741.1
Arabidopsis thaliana (thale cress)	AT3G18430	NP_001189924.1
Oryza sativa (rice)	Os08g0442300	NP_001061916.2
Saccharomyces cerevisiae (baker's yeast)	CNB1	NP_012731.1
Xenopus tropicalis (tropical clawed frog)	ppp3r1	NP_989400.1
Homo sapiens (human)	PPP3R1	NP_000936.1
Danio rerio (zebrafish)	ppp3r1a	XP_692770.2
Drosophila melanogaster (fruit fly)	CanB2	NP_524874.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004298	XP_313573.5
Caenorhabditis elegans (roundworm)	cnb-1	NP_001256317.1
Kluyveromyces lactis	KLLA0D02992g	XP_453201.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicate a role for Cn and calcium-dependent signal transduction in sleep and memory regulation.
Title: Pan-neuronal knockdown of calcineurin reduces sleep in the fruit fly, Drosophila melanogaster.

calcineurin, upon activation by Ca(2+)-calmodulin, cooperates with other factors to negatively regulate Egf receptor signaling at the level of sprouty and the GTPase-activating protein Gap1
Title: The Ca(2+)-calmodulin-activated protein phosphatase calcineurin negatively regulates EGF receptor signaling in Drosophila development.

The following CanB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CanB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02332	NM_080002.4 Latest version!	Drosophila melanogaster calcineurin B, transcript variant A (CanB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa02332	NM_001258602.1 Latest version!	Drosophila melanogaster calcineurin B, transcript variant B (CanB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CanB ( NM_080002.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524741.1 Drosophila melanogaster calcineurin B, transcript variant A (CanB), mRNA.

Price & Availability↑

CloneID	OFa02332
Clone ID Related Accession (Same CDS sequence)	NM_080002.4 , NM_001258602.1
Accession Version	NM_080002.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	calcineurin B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080002.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAACG AGACCTCCCT GCCCATGGAC ATGTGTTCCA ACTTCGATGC GGACGAGATC 
CGTAGGCTGG GCAAGCGATT CCGCAAGCTG GATCTGGACA ACTCGGGTGC GCTGAGCATA 
GATGAGTTCA TGTCGCTGCC GGAATTGCAG CAGAATCCGC TGGTGCAGCG GGTTATCGAT 
ATCTTCGATG CGGATGGAAA CGGGGAGGTG GACTTCAAGG AGTTTATCCA GGGAGTGTCG 
CAGTTTAGCG TGCGCGGCGA TAAGCTGTCC AAGCTGCGAT TCGCCTTCCG CATCTATGAT 
ATGGACAATG ATGGCTACAT CTCGAACGGG GAACTCTTCC AGGTGCTCAA GATGATGGTG 
GGCAACAATC TGAAGGACAC ACAGCTGCAG CAGATCGTGG ACAAGACCAT TTGCTTTGCG 
GACAAGGACG AGGATGGCAA GATATCGTTC GATGAATTCT GCTCGGTGGT GGGCAATACC 
GATATTCACA AGAAAATGGT TGTCGATGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524741.1
CDS	170..682
Translation	MGNETSLPMDMCSNFDADEIRRLGKRFRKLDLDNSGALSIDEFMSLPELQQNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVRGDKLSKLRFAFRIYDMDNDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTICFADKDEDGKISFDEFCSVVGNTDIHKKMVVDV

Target ORF information:

RefSeq Version	NM_080002.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster calcineurin B, transcript variant A (CanB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080002.4

ORF Insert Sequence:

ATGGGTAACG AGACCTCCCT GCCCATGGAC ATGTGTTCCA ACTTCGATGC GGACGAGATC 
CGTAGGCTGG GCAAGCGATT CCGCAAGCTG GATCTGGACA ACTCGGGTGC GCTGAGCATA 
GATGAGTTCA TGTCGCTGCC GGAATTGCAG CAGAATCCGC TGGTGCAGCG GGTTATCGAT 
ATCTTCGATG CGGATGGAAA CGGGGAGGTG GACTTCAAGG AGTTTATCCA GGGAGTGTCG 
CAGTTTAGCG TGCGCGGCGA TAAGCTGTCC AAGCTGCGAT TCGCCTTCCG CATCTATGAT 
ATGGACAATG ATGGCTACAT CTCGAACGGG GAACTCTTCC AGGTGCTCAA GATGATGGTG 
GGCAACAATC TGAAGGACAC ACAGCTGCAG CAGATCGTGG ACAAGACCAT TTGCTTTGCG 
GACAAGGACG AGGATGGCAA GATATCGTTC GATGAATTCT GCTCGGTGGT GGGCAATACC 
GATATTCACA AGAAAATGGT TGTCGATGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CanB ( NM_001258602.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245531.1 Drosophila melanogaster calcineurin B, transcript variant B (CanB), mRNA.

Price & Availability↑

CloneID	OFa02332
Clone ID Related Accession (Same CDS sequence)	NM_080002.4 , NM_001258602.1
Accession Version	NM_001258602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	calcineurin B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAACG AGACCTCCCT GCCCATGGAC ATGTGTTCCA ACTTCGATGC GGACGAGATC 
CGTAGGCTGG GCAAGCGATT CCGCAAGCTG GATCTGGACA ACTCGGGTGC GCTGAGCATA 
GATGAGTTCA TGTCGCTGCC GGAATTGCAG CAGAATCCGC TGGTGCAGCG GGTTATCGAT 
ATCTTCGATG CGGATGGAAA CGGGGAGGTG GACTTCAAGG AGTTTATCCA GGGAGTGTCG 
CAGTTTAGCG TGCGCGGCGA TAAGCTGTCC AAGCTGCGAT TCGCCTTCCG CATCTATGAT 
ATGGACAATG ATGGCTACAT CTCGAACGGG GAACTCTTCC AGGTGCTCAA GATGATGGTG 
GGCAACAATC TGAAGGACAC ACAGCTGCAG CAGATCGTGG ACAAGACCAT TTGCTTTGCG 
GACAAGGACG AGGATGGCAA GATATCGTTC GATGAATTCT GCTCGGTGGT GGGCAATACC 
GATATTCACA AGAAAATGGT TGTCGATGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245531.1
CDS	104..616
Translation	MGNETSLPMDMCSNFDADEIRRLGKRFRKLDLDNSGALSIDEFMSLPELQQNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVRGDKLSKLRFAFRIYDMDNDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTICFADKDEDGKISFDEFCSVVGNTDIHKKMVVDV

Target ORF information:

RefSeq Version	NM_001258602.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster calcineurin B, transcript variant B (CanB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258602.1

ORF Insert Sequence:

ATGGGTAACG AGACCTCCCT GCCCATGGAC ATGTGTTCCA ACTTCGATGC GGACGAGATC 
CGTAGGCTGG GCAAGCGATT CCGCAAGCTG GATCTGGACA ACTCGGGTGC GCTGAGCATA 
GATGAGTTCA TGTCGCTGCC GGAATTGCAG CAGAATCCGC TGGTGCAGCG GGTTATCGAT 
ATCTTCGATG CGGATGGAAA CGGGGAGGTG GACTTCAAGG AGTTTATCCA GGGAGTGTCG 
CAGTTTAGCG TGCGCGGCGA TAAGCTGTCC AAGCTGCGAT TCGCCTTCCG CATCTATGAT 
ATGGACAATG ATGGCTACAT CTCGAACGGG GAACTCTTCC AGGTGCTCAA GATGATGGTG 
GGCAACAATC TGAAGGACAC ACAGCTGCAG CAGATCGTGG ACAAGACCAT TTGCTTTGCG 
GACAAGGACG AGGATGGCAA GATATCGTTC GATGAATTCT GCTCGGTGGT GGGCAATACC 
GATATTCACA AGAAAATGGT TGTCGATGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CanB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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