Vha16-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vha16-1
Entrez Gene ID	44307
Full Name	Vacuolar H[+] ATPase 16kD subunit 1
Synonyms	ATP6V0C,BcDNA:SD02875,CG3161,Dmel\CG3161,DucC,EP2372,MRE15,V-ATPase,Vha1,Vha16,Vhac,vha16,vha16-1
General protein information	Preferred Names Vacuolar H[+] ATPase 16kD subunit 1 Names CG3161 gene product from transcript CG3161-RA CG3161-PA CG3161-PB CG3161-PC CG3161-PD Vha16-1-PA Vha16-1-PB Vha16-1-PC Vha16-1-PD c subunit ductin ductin subunit C ductin, vacuolar H(+)-ATPase subunit C proteolipid mRNA-like ncRNA in embryogenesis 15 vacuolar H[+] ATPase 16kD subunit vacuolar H[+] ATPase 16kD subunit 1 vacuolar H[+] ATPase subunit 16-1 vacuolar H[+]-ATPase c subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42B2-42B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057453.5	NP_476801.1	vacuolar H[+] ATPase 16kD subunit 1, isoform A
NM_165475.3	NP_724475.1	vacuolar H[+] ATPase 16kD subunit 1, isoform C
NM_165476.3	NP_724476.1	vacuolar H[+] ATPase 16kD subunit 1, isoform D
NM_165474.3	NP_724474.1	vacuolar H[+] ATPase 16kD subunit 1, isoform B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	atp6v0cb	NP_991117.1
Bos taurus (cattle)	ATP6V0C	NP_001017954.1
Rattus norvegicus (Norway rat)	Atp6v0c	NP_570836.1
Danio rerio (zebrafish)	atp6v0ca	NP_001098606.2
Drosophila melanogaster (fruit fly)	Vha16-3	NP_001189086.1
Saccharomyces cerevisiae (baker's yeast)	VMA3	NP_010887.3
Eremothecium gossypii	AGOS_AEL074W	NP_984787.1
Neurospora crassa	NCU01332	XP_961418.2
Homo sapiens (human)	ATP6V0C	NP_001185498.1
Canis lupus familiaris (dog)	ATP6V0C	XP_537002.1
Gallus gallus (chicken)	ATP6V0C	NP_001264953.1
Caenorhabditis elegans (roundworm)	vha-2	NP_499166.1
Schizosaccharomyces pombe (fission yeast)	vma3	NP_594799.1
Pan troglodytes (chimpanzee)	ATP6V0C	XP_510748.3
Drosophila melanogaster (fruit fly)	Vha16-1	NP_724476.1
Caenorhabditis elegans (roundworm)	vha-1	NP_499165.1
Magnaporthe oryzae (rice blast fungus)	MGG_02466	XP_003709260.1
Oryza sativa (rice)	Os01g0962300	NP_001045477.1
	ATP6V0C	XP_001088617.1
Mus musculus (house mouse)	Atp6v0c	NP_033859.1
Caenorhabditis elegans (roundworm)	vha-3	NP_500188.1
Kluyveromyces lactis	KLLA0E22441g	XP_454966.1
Xenopus tropicalis (tropical clawed frog)	atp6v0c	NP_988893.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

lysosomal-associated functions regulated by the TFEB-V-ATPase axis might play a conserved role in shaping cell fate.
Title: Control of lysosomal biogenesis and Notch-dependent tissue patterning by components of the TFEB-V-ATPase axis in Drosophila melanogaster.

The following Vha16-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vha16-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11344	NM_057453.5 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant A (Vha16-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11344	NM_165474.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant B (Vha16-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11344	NM_165475.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant C (Vha16-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11344	NM_165476.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant D (Vha16-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vha16-1 ( NM_057453.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476801.1 Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant A (Vha16-1), mRNA.

Price & Availability↑

CloneID	OFa11344
Clone ID Related Accession (Same CDS sequence)	NM_057453.5 , NM_165475.3 , NM_165476.3 , NM_165474.3
Accession Version	NM_057453.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 16kD subunit 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057453.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCTG AAGTGAGCAG CGACAACCCC ATCTATGGCC CCTTCTTCGG AGTTATGGGA 
GCCGCCTCCG CCATCATCTT CTCGGCCCTG GGCGCTGCTT ATGGCACTGC TAAGTCTGGT 
ACCGGTATTG CTGCCATGTC AGTGATGCGG CCTGAACTGA TCATGAAATC CATCATTCCT 
GTGGTCATGG CGGGTATCAT TGCCATTTAC GGTCTGGTGG TGGCTGTGCT TATTGCCGGC 
GCGCTGGAAG AGCCATCAAA GTACTCACTA TACAGGGGCT TCATTCACTT GGGAGCCGGT 
CTGGCGGTGG GCTTCTCTGG TCTGGCAGCT GGTTTTGCGA TCGGCATCGT GGGAGACGCC 
GGTGTCCGTG GCACAGCACA GCAGCCTAGA CTGTTCGTGG GCATGATCCT GATTCTCATC 
TTCGCCGAAG TGTTGGGTCT CTACGGCTTG ATTGTGGCCA TTTACCTGTA CACGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476801.1
CDS	126..605
Translation	MSSEVSSDNPIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVAVLIAGALEEPSKYSLYRGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVAIYLYTK

Target ORF information:

RefSeq Version	NM_057453.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant A (Vha16-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057453.5

ORF Insert Sequence:

ATGTCTTCTG AAGTGAGCAG CGACAACCCC ATCTATGGCC CCTTCTTCGG AGTTATGGGA 
GCCGCCTCCG CCATCATCTT CTCGGCCCTG GGCGCTGCTT ATGGCACTGC TAAGTCTGGT 
ACCGGTATTG CTGCCATGTC AGTGATGCGG CCTGAACTGA TCATGAAATC CATCATTCCT 
GTGGTCATGG CGGGTATCAT TGCCATTTAC GGTCTGGTGG TGGCTGTGCT TATTGCCGGC 
GCGCTGGAAG AGCCATCAAA GTACTCACTA TACAGGGGCT TCATTCACTT GGGAGCCGGT 
CTGGCGGTGG GCTTCTCTGG TCTGGCAGCT GGTTTTGCGA TCGGCATCGT GGGAGACGCC 
GGTGTCCGTG GCACAGCACA GCAGCCTAGA CTGTTCGTGG GCATGATCCT GATTCTCATC 
TTCGCCGAAG TGTTGGGTCT CTACGGCTTG ATTGTGGCCA TTTACCTGTA CACGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha16-1 ( NM_165475.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724475.1 Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant C (Vha16-1), mRNA.

Price & Availability↑

CloneID	OFa11344
Clone ID Related Accession (Same CDS sequence)	NM_057453.5 , NM_165475.3 , NM_165476.3 , NM_165474.3
Accession Version	NM_165475.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 16kD subunit 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165475.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCTG AAGTGAGCAG CGACAACCCC ATCTATGGCC CCTTCTTCGG AGTTATGGGA 
GCCGCCTCCG CCATCATCTT CTCGGCCCTG GGCGCTGCTT ATGGCACTGC TAAGTCTGGT 
ACCGGTATTG CTGCCATGTC AGTGATGCGG CCTGAACTGA TCATGAAATC CATCATTCCT 
GTGGTCATGG CGGGTATCAT TGCCATTTAC GGTCTGGTGG TGGCTGTGCT TATTGCCGGC 
GCGCTGGAAG AGCCATCAAA GTACTCACTA TACAGGGGCT TCATTCACTT GGGAGCCGGT 
CTGGCGGTGG GCTTCTCTGG TCTGGCAGCT GGTTTTGCGA TCGGCATCGT GGGAGACGCC 
GGTGTCCGTG GCACAGCACA GCAGCCTAGA CTGTTCGTGG GCATGATCCT GATTCTCATC 
TTCGCCGAAG TGTTGGGTCT CTACGGCTTG ATTGTGGCCA TTTACCTGTA CACGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724475.1
CDS	274..753
Translation	MSSEVSSDNPIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVAVLIAGALEEPSKYSLYRGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVAIYLYTK

Target ORF information:

RefSeq Version	NM_165475.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant C (Vha16-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165475.3

ORF Insert Sequence:

ATGTCTTCTG AAGTGAGCAG CGACAACCCC ATCTATGGCC CCTTCTTCGG AGTTATGGGA 
GCCGCCTCCG CCATCATCTT CTCGGCCCTG GGCGCTGCTT ATGGCACTGC TAAGTCTGGT 
ACCGGTATTG CTGCCATGTC AGTGATGCGG CCTGAACTGA TCATGAAATC CATCATTCCT 
GTGGTCATGG CGGGTATCAT TGCCATTTAC GGTCTGGTGG TGGCTGTGCT TATTGCCGGC 
GCGCTGGAAG AGCCATCAAA GTACTCACTA TACAGGGGCT TCATTCACTT GGGAGCCGGT 
CTGGCGGTGG GCTTCTCTGG TCTGGCAGCT GGTTTTGCGA TCGGCATCGT GGGAGACGCC 
GGTGTCCGTG GCACAGCACA GCAGCCTAGA CTGTTCGTGG GCATGATCCT GATTCTCATC 
TTCGCCGAAG TGTTGGGTCT CTACGGCTTG ATTGTGGCCA TTTACCTGTA CACGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha16-1 ( NM_165476.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724476.1 Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant D (Vha16-1), mRNA.

Price & Availability↑

CloneID	OFa11344
Clone ID Related Accession (Same CDS sequence)	NM_057453.5 , NM_165475.3 , NM_165476.3 , NM_165474.3
Accession Version	NM_165476.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 16kD subunit 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165476.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCTG AAGTGAGCAG CGACAACCCC ATCTATGGCC CCTTCTTCGG AGTTATGGGA 
GCCGCCTCCG CCATCATCTT CTCGGCCCTG GGCGCTGCTT ATGGCACTGC TAAGTCTGGT 
ACCGGTATTG CTGCCATGTC AGTGATGCGG CCTGAACTGA TCATGAAATC CATCATTCCT 
GTGGTCATGG CGGGTATCAT TGCCATTTAC GGTCTGGTGG TGGCTGTGCT TATTGCCGGC 
GCGCTGGAAG AGCCATCAAA GTACTCACTA TACAGGGGCT TCATTCACTT GGGAGCCGGT 
CTGGCGGTGG GCTTCTCTGG TCTGGCAGCT GGTTTTGCGA TCGGCATCGT GGGAGACGCC 
GGTGTCCGTG GCACAGCACA GCAGCCTAGA CTGTTCGTGG GCATGATCCT GATTCTCATC 
TTCGCCGAAG TGTTGGGTCT CTACGGCTTG ATTGTGGCCA TTTACCTGTA CACGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724476.1
CDS	109..588
Translation	MSSEVSSDNPIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVAVLIAGALEEPSKYSLYRGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVAIYLYTK

Target ORF information:

RefSeq Version	NM_165476.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant D (Vha16-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165476.3

ORF Insert Sequence:

ATGTCTTCTG AAGTGAGCAG CGACAACCCC ATCTATGGCC CCTTCTTCGG AGTTATGGGA 
GCCGCCTCCG CCATCATCTT CTCGGCCCTG GGCGCTGCTT ATGGCACTGC TAAGTCTGGT 
ACCGGTATTG CTGCCATGTC AGTGATGCGG CCTGAACTGA TCATGAAATC CATCATTCCT 
GTGGTCATGG CGGGTATCAT TGCCATTTAC GGTCTGGTGG TGGCTGTGCT TATTGCCGGC 
GCGCTGGAAG AGCCATCAAA GTACTCACTA TACAGGGGCT TCATTCACTT GGGAGCCGGT 
CTGGCGGTGG GCTTCTCTGG TCTGGCAGCT GGTTTTGCGA TCGGCATCGT GGGAGACGCC 
GGTGTCCGTG GCACAGCACA GCAGCCTAGA CTGTTCGTGG GCATGATCCT GATTCTCATC 
TTCGCCGAAG TGTTGGGTCT CTACGGCTTG ATTGTGGCCA TTTACCTGTA CACGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha16-1 ( NM_165474.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724474.1 Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant B (Vha16-1), mRNA.

Price & Availability↑

CloneID	OFa11344
Clone ID Related Accession (Same CDS sequence)	NM_057453.5 , NM_165475.3 , NM_165476.3 , NM_165474.3
Accession Version	NM_165474.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 16kD subunit 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165474.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCTG AAGTGAGCAG CGACAACCCC ATCTATGGCC CCTTCTTCGG AGTTATGGGA 
GCCGCCTCCG CCATCATCTT CTCGGCCCTG GGCGCTGCTT ATGGCACTGC TAAGTCTGGT 
ACCGGTATTG CTGCCATGTC AGTGATGCGG CCTGAACTGA TCATGAAATC CATCATTCCT 
GTGGTCATGG CGGGTATCAT TGCCATTTAC GGTCTGGTGG TGGCTGTGCT TATTGCCGGC 
GCGCTGGAAG AGCCATCAAA GTACTCACTA TACAGGGGCT TCATTCACTT GGGAGCCGGT 
CTGGCGGTGG GCTTCTCTGG TCTGGCAGCT GGTTTTGCGA TCGGCATCGT GGGAGACGCC 
GGTGTCCGTG GCACAGCACA GCAGCCTAGA CTGTTCGTGG GCATGATCCT GATTCTCATC 
TTCGCCGAAG TGTTGGGTCT CTACGGCTTG ATTGTGGCCA TTTACCTGTA CACGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724474.1
CDS	91..570
Translation	MSSEVSSDNPIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVAVLIAGALEEPSKYSLYRGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVAIYLYTK

Target ORF information:

RefSeq Version	NM_165474.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 16kD subunit 1, transcript variant B (Vha16-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165474.3

ORF Insert Sequence:

ATGTCTTCTG AAGTGAGCAG CGACAACCCC ATCTATGGCC CCTTCTTCGG AGTTATGGGA 
GCCGCCTCCG CCATCATCTT CTCGGCCCTG GGCGCTGCTT ATGGCACTGC TAAGTCTGGT 
ACCGGTATTG CTGCCATGTC AGTGATGCGG CCTGAACTGA TCATGAAATC CATCATTCCT 
GTGGTCATGG CGGGTATCAT TGCCATTTAC GGTCTGGTGG TGGCTGTGCT TATTGCCGGC 
GCGCTGGAAG AGCCATCAAA GTACTCACTA TACAGGGGCT TCATTCACTT GGGAGCCGGT 
CTGGCGGTGG GCTTCTCTGG TCTGGCAGCT GGTTTTGCGA TCGGCATCGT GGGAGACGCC 
GGTGTCCGTG GCACAGCACA GCAGCCTAGA CTGTTCGTGG GCATGATCCT GATTCTCATC 
TTCGCCGAAG TGTTGGGTCT CTACGGCTTG ATTGTGGCCA TTTACCTGTA CACGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vha16-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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