Sara cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sara
Entrez Gene ID	44263
Full Name	Smad anchor for receptor activation
Synonyms	CG15667,Dmel\CG15667,SARA,dSARA,sara
General protein information	Preferred Names Smad anchor for receptor activation Names CG15667 gene product from transcript CG15667-RA CG15667-PA Sara-PA smad anchor for receptor activation
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57E6-57E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_079990.3	NP_524729.2	smad anchor for receptor activation

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Sara	NP_524729.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004049	XP_309600.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Notch binds Uninflatable, and both traffic together through Sara endosomes, which is essential to direct asymmetric endosomes motility and Notch-dependent cell fate assignation.
Title: Uninflatable and Notch control the targeting of Sara endosomes during asymmetric division.

Sara itself contributes to the control of the intestinal stem cells asymmetric division.
Title: Sara endosomes and the asymmetric division of intestinal stem cells.

asymmetric targeting of Delta and Notch-containing Sara endosomes will increase Notch signalling in sensory organ precursor daughter cell, pIIa, and decrease it in pIIb
Title: Directional Delta and Notch trafficking in Sara endosomes during asymmetric cell division.

During mitosis, Sara endosomes associate with the spindle machinery to segregate into the two daughter cells, daughter cells thereby inherited equal amounts of signaling molecules and retained the Dpp signaling levels of the mother cell
Title: Sara endosomes and the maintenance of Dpp signaling levels across mitosis.

PP1 binds Sara and negatively regulates Dpp signaling in Drosophila melanogaster.
Title: PP1 binds Sara and negatively regulates Dpp signaling in Drosophila melanogaster.

The following Sara gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sara cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11339	NM_079990.3 Latest version!	Drosophila melanogaster smad anchor for receptor activation (Sara), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sara ( NM_079990.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524729.2 Drosophila melanogaster smad anchor for receptor activation (Sara), mRNA.

Price & Availability↑

CloneID	OFa11339
Clone ID Related Accession (Same CDS sequence)	NM_079990.3
Accession Version	NM_079990.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	smad anchor for receptor activation
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079990.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTTGG TGGACATTGA TCAGGTTTTG GACAATCTGG AACTGAGCAT TGCCGCCGAT 
GAGCAGCTGA GTAGAAATGC AGCGGCAGCA GCAGCTGCAG GTGCAAATCC TGGCCGTTTT 
CTGGGCGACG GAGCAAGTGC ATCGATCAGT GGAGTTGGAG CTGGCGCCGG AGTATCGGGA 
GCTGCGGGAG TAACACCAAC ACCGGCGTCT GGAAAGTCCT TTGTGGGCGT CTCTCAGGTT 
TTCAACAGCC TGGATGACTA CAGGGAAAAT GTGAAGTCCT TGCAGGTGGA TGCCCAATTA 
CCCAGCTACG ACTGGCAAAC GGAGGCGGAA CACGAAGACG ACATCCACAA GTTCAGCGAA 
CAGGCGTCCA AAAATAACGG CCAGACGATA GTATCGTCCT CCGAATCGCT GACCACCTCA 
TCCTGTTCCA CCTTCTCGTC GGGTCCGTCG CCCGTGAGCA ATGGCAGTCA CTCGGAGGAG 
CTAGCAACGC CCCCCGAAGA TGAGAAAACG AGTGAAGTTC AGGTGGAGCC AAAGGAACAG 
GATCAACAGC CGGAACAGGT CAAGCATGAG GACCAGCACC AAGATCAGGC TGTTCCACCG 
CCAACGCAAT CCAAACAAAC TGTAGAGGAG TTAGCAGTTG GATTGTCATC CATCTCCATC 
ACTGCGTCCG GCACAGAATC GCAATCCCTA TTGCCGGAGG AGGTCACCGC ATCGTCTGTT 
CTCGGGCAGG TCCTCGAGGA GCTCTCGGAG GAATCGTCGC CCAGTAGAGA ACCCGAAACC 
GAAATGACCA ACGGCATAGA TGTCCCATTG GCCAATTTAC CAGCATCTGT TGCGGAAGTA 
CCGGTCCCTA AAGAGATGGA GCAGCCAGTA CAAACGGAAG AAGAGGATGT GGAGCTGCCA 
CAGGAGCAGC ATCCCAGGCG TAGTCAACCG TTGACCTTCT GCTCCACCAT GGACGAGATC 
TCGGACACCG AACTGGACAG CATGCTGCAA GAGATGGATG CAGATGACAA CCAGGCGGAC 
GATCAGCAAA TGCCCGACGA TGAGAAATCC TGCTCCGCCT CGCCAGTGAC CGAGGAGGAG 
TCGGTGCGGA TTCTATCCGA TGAGCAGGAG ACAACCTCGA ACGATCAGAT GAACGTGGAC 
AACTTTTCGC AGGCCTCCAC TGTGGAGTTT GCTGATCTGA AGCAGCCGGA GTCCACACCA 
GTTACCGCGA TGGGAGAAGA TGTAGCCTCC AGTTTCAGCA GCACGGAATC GGATTCCCTT 
TCGGGCAGGA AACTAGATGG CGAAGTGGAG GTCGATCGAG AGCATGAAGA TGCCAGCCTG 
GCAACGGATG CCCTGGAAAC CCAAGAGCAG CGCCCCCAGC GACCACAAAC CCTAAATTTG 
AATGGTTGCC AGGCAGAAAG TTCCACGCCA TCTCAAGATG AAAATCCCGA AATTCAACAG 
GAAACTTTGC AGCCTACCGA AGTCAGGGAA GGAGCGGCAG GAGTAGGTGT TATACCCGGC 
GAGGATGACG AGGAAAGTCC TATCTACGAA GCAGTGGGCT ACAGCGATCC GCATGCCAAT 
CTGGGCAAGG TGCCACCCAT ATGGGTCCCG GATAACATGG CCGGGCAGTG CATGCAATGT 
CAGCAAAAGT TCACCATGAT CAAAAGGCGT CACCACTGTC GCGCCTGCGG AAAGGTCTTG 
TGCTCTGTCT GCTGTTCGCA ACGATTCCGA TTGGAATTCG CCACCGAACC CGAGTCTAGG 
GTTTGTGTGC AGTGTTATAT GATTCTCTCT GAGCGGCAGG CTAATGGATT GAATTCTGAG 
TCAGCACCGG GTTCGGCATT GCCGCCCACT CCCATTCGTT CGCCAAACCC AAACAATCCA 
ATGGAATATT GTTCTACAAT ACCACCACAC CGTCAGGTGG CCAACTCACC GGGTGCTCCG 
CCACCTAGTG TGATTGTGCC CGTGGGTGTG TTGAAAAAAA CCGATGGCAG CTCCTCCAAT 
TCCTCCAGTT CGGATGGCCA AAAGGGCCAA AGAAAGCGAA AGAGTGTTAT GTTCAGTGAT 
GGCATTGCGC CCGGCAGCGA ACTGGCCAGT ACCATGGAGC AGCAATGGGG CGAGTCCAAG 
CATTCTCGAA GGGGACTATC CAGGAGCGGC TCGGGAGCTG GTCAAAAGCC ACCAACGCCT 
GCGACAGAGG AGCCACCTCG CAGTATTGAC ACTAGCTCTA CATTGGGCCT AGTGGCACAG 
CTATTCCGCG GTTCCATTCC ACCAAGCGCA GCGGCTGCCA CATTATCGGC TACCGAGACA 
AGCGCTGGTC GCTCCTCATC CCAATCGCAG GCACAGACCT CGCCGCGTCG CAAAGCGGAA 
TCATCACGGA ATCGCAAACT ACCGCCAAAC GGAGATGCCC AAGGCTGCTA TCTGCCGCCG 
GAGGAGAACG GCCTGCCCCC AATTTGTATA AGCAGCAAAG AGGGCGGTGA GGTGGACTAC 
AAAGTAGTGC GCAACGATGG ATCACTCCTA GATCGATTGC AGAACGAAAC GCTCAAGTTC 
ATTATAAAGA ACAACTTCTT TGTGCTGGTC AAGATTGTTA ACATGAGCTG CTGTATGAAT 
CGATGGGTGC TGAACTTTAC GACATCCGGA CTACATCATA TGGGCAGCGA TGAGTTGATC 
ATTCTGCTGG AGATTAAGCA ACCCAAGCAA GAAGAATACG TGAAGGGGGA AGTCGCAATT 
CCGAAAGATT TGTTCCAGCA CCTGTACGAG ATCTACGTGG AGGCGGAGCA TGCCCGGTCC 
GGCACCCATG AGTTGGCTTT CACCAGTCCG CGAAATGCCA ATTTTCTGGG TTCCCGGGAG 
CATGGAGGAT TTATCTTTAT TCGGCCAACG TACCAGTGCT TGCAGGGAGT TATTGTACCA 
GACAATCCGT ATCTGGTTGG CGTGCTCATC CATAGACACG AGGTACCTTG GGCTAAGGTG 
CTACCTTTGC GGTTGATCCT CCGCTTGGGT GCCCAGTATC GGTACTATCC CTGTCCATTG 
ATCTCGGTGC GCGGCAGGGA GTCGGTGTAT GGCGAAATAG CTCAAACTAT CATTAACTTT 
CTGGTGGATT TTCGCAACTA TTCGTACTCT CTACCGGCCA TCCGAGGATT ATATATCCAC 
ATGGAGGATA GGCAGACGAC GGTGATTATT CCAAAGTACC GCCAGCAGGA TGTAATTAAG 
GCAATCAATA ATGCTAGTGA TCATATACTG GCTTTTGCTG GCAACTTCTC AAAAGTGGCC 
GATGGTCACT TGGTTTGCAT GCAGAATATC AACGACGATC GATCCGAGAT GTACTCCTAC 
TCCACGCAGG CGATTAATAT TCAGGGTCAA CCCAGGAAAA TCACTGGAGC TAGCTTCTTT 
GTGCTAAATG AAGCCCTCAA GAGCAGCAGT GGCTTGTCGG GTAAGTGCAG CATCGTGGAG 
GATGGCCTAA TGGTACAGAT TATGCCTGCC AAAATGGAGG AAGTGCGTCA GGCGTTGCGC 
AACCAAAACG ACATCGACAT CGTCTGTGGA CCCATTGACG CCACCGACGA TCAGAGTGAG 
ATTGTAAGCA TTAAGTGGGT GGACAATAAT CGTGACATCA ATGTGGGGGT TAAGTCGCCC 
ATTGATGACC AGCCCATGGA TGGCATTTCA AACATGCGAG TGCTGAAATG GGCCAGCTTT 
AACTATTCCA ATACCAACTA TGCCATCCGC CTGAGTGATA TCTACATTAT TAAGTGCGAG 
GACTGGTATG CAACAAATGG TGGAAACTGT GAGGAGATAA CACGGATTGC GGAGCAACTG 
GCTCGCAGTG CCTCGATGGC TCTGCTGCCA CTTCTCGATC TTTTAGCAGC TGCTGGCATT 
AATAAGCTTG GCCTAAGAGC CACGCTCGAT CAGGATAACG TGGGCTATGA AGCCGGAGCT 
CGTGGCTCCA AGCTACCGCC GTTGTACATG AACGCGCTGG ACAACCATCT GATAGCCACG 
CTGCATGGCG AATCCTCCGG CATTCAAGAG CCCATCATTC TAGAGCTGAT CTTTTACATA 
CTGAACGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524729.2
CDS	188..4219
Translation	MDLVDIDQVLDNLELSIAADEQLSRNAAAAAAAGANPGRFLGDGASASISGVGAGAGVSGAAGVTPTPASGKSFVGVSQVFNSLDDYRENVKSLQVDAQLPSYDWQTEAEHEDDIHKFSEQASKNNGQTIVSSSESLTTSSCSTFSSGPSPVSNGSHSEELATPPEDEKTSEVQVEPKEQDQQPEQVKHEDQHQDQAVPPPTQSKQTVEELAVGLSSISITASGTESQSLLPEEVTASSVLGQVLEELSEESSPSREPETEMTNGIDVPLANLPASVAEVPVPKEMEQPVQTEEEDVELPQEQHPRRSQPLTFCSTMDEISDTELDSMLQEMDADDNQADDQQMPDDEKSCSASPVTEEESVRILSDEQETTSNDQMNVDNFSQASTVEFADLKQPESTPVTAMGEDVASSFSSTESDSLSGRKLDGEVEVDREHEDASLATDALETQEQRPQRPQTLNLNGCQAESSTPSQDENPEIQQETLQPTEVREGAAGVGVIPGEDDEESPIYEAVGYSDPHANLGKVPPIWVPDNMAGQCMQCQQKFTMIKRRHHCRACGKVLCSVCCSQRFRLEFATEPESRVCVQCYMILSERQANGLNSESAPGSALPPTPIRSPNPNNPMEYCSTIPPHRQVANSPGAPPPSVIVPVGVLKKTDGSSSNSSSSDGQKGQRKRKSVMFSDGIAPGSELASTMEQQWGESKHSRRGLSRSGSGAGQKPPTPATEEPPRSIDTSSTLGLVAQLFRGSIPPSAAAATLSATETSAGRSSSQSQAQTSPRRKAESSRNRKLPPNGDAQGCYLPPEENGLPPICISSKEGGEVDYKVVRNDGSLLDRLQNETLKFIIKNNFFVLVKIVNMSCCMNRWVLNFTTSGLHHMGSDELIILLEIKQPKQEEYVKGEVAIPKDLFQHLYEIYVEAEHARSGTHELAFTSPRNANFLGSREHGGFIFIRPTYQCLQGVIVPDNPYLVGVLIHRHEVPWAKVLPLRLILRLGAQYRYYPCPLISVRGRESVYGEIAQTIINFLVDFRNYSYSLPAIRGLYIHMEDRQTTVIIPKYRQQDVIKAINNASDHILAFAGNFSKVADGHLVCMQNINDDRSEMYSYSTQAINIQGQPRKITGASFFVLNEALKSSSGLSGKCSIVEDGLMVQIMPAKMEEVRQALRNQNDIDIVCGPIDATDDQSEIVSIKWVDNNRDINVGVKSPIDDQPMDGISNMRVLKWASFNYSNTNYAIRLSDIYIIKCEDWYATNGGNCEEITRIAEQLARSASMALLPLLDLLAAAGINKLGLRATLDQDNVGYEAGARGSKLPPLYMNALDNHLIATLHGESSGIQEPIILELIFYILNA

Target ORF information:

RefSeq Version	NM_079990.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster smad anchor for receptor activation (Sara), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079990.3

ORF Insert Sequence:

ATGGACTTGG TGGACATTGA TCAGGTTTTG GACAATCTGG AACTGAGCAT TGCCGCCGAT 
GAGCAGCTGA GTAGAAATGC AGCGGCAGCA GCAGCTGCAG GTGCAAATCC TGGCCGTTTT 
CTGGGCGACG GAGCAAGTGC ATCGATCAGT GGAGTTGGAG CTGGCGCCGG AGTATCGGGA 
GCTGCGGGAG TAACACCAAC ACCGGCGTCT GGAAAGTCCT TTGTGGGCGT CTCTCAGGTT 
TTCAACAGCC TGGATGACTA CAGGGAAAAT GTGAAGTCCT TGCAGGTGGA TGCCCAATTA 
CCCAGCTACG ACTGGCAAAC GGAGGCGGAA CACGAAGACG ACATCCACAA GTTCAGCGAA 
CAGGCGTCCA AAAATAACGG CCAGACGATA GTATCGTCCT CCGAATCGCT GACCACCTCA 
TCCTGTTCCA CCTTCTCGTC GGGTCCGTCG CCCGTGAGCA ATGGCAGTCA CTCGGAGGAG 
CTAGCAACGC CCCCCGAAGA TGAGAAAACG AGTGAAGTTC AGGTGGAGCC AAAGGAACAG 
GATCAACAGC CGGAACAGGT CAAGCATGAG GACCAGCACC AAGATCAGGC TGTTCCACCG 
CCAACGCAAT CCAAACAAAC TGTAGAGGAG TTAGCAGTTG GATTGTCATC CATCTCCATC 
ACTGCGTCCG GCACAGAATC GCAATCCCTA TTGCCGGAGG AGGTCACCGC ATCGTCTGTT 
CTCGGGCAGG TCCTCGAGGA GCTCTCGGAG GAATCGTCGC CCAGTAGAGA ACCCGAAACC 
GAAATGACCA ACGGCATAGA TGTCCCATTG GCCAATTTAC CAGCATCTGT TGCGGAAGTA 
CCGGTCCCTA AAGAGATGGA GCAGCCAGTA CAAACGGAAG AAGAGGATGT GGAGCTGCCA 
CAGGAGCAGC ATCCCAGGCG TAGTCAACCG TTGACCTTCT GCTCCACCAT GGACGAGATC 
TCGGACACCG AACTGGACAG CATGCTGCAA GAGATGGATG CAGATGACAA CCAGGCGGAC 
GATCAGCAAA TGCCCGACGA TGAGAAATCC TGCTCCGCCT CGCCAGTGAC CGAGGAGGAG 
TCGGTGCGGA TTCTATCCGA TGAGCAGGAG ACAACCTCGA ACGATCAGAT GAACGTGGAC 
AACTTTTCGC AGGCCTCCAC TGTGGAGTTT GCTGATCTGA AGCAGCCGGA GTCCACACCA 
GTTACCGCGA TGGGAGAAGA TGTAGCCTCC AGTTTCAGCA GCACGGAATC GGATTCCCTT 
TCGGGCAGGA AACTAGATGG CGAAGTGGAG GTCGATCGAG AGCATGAAGA TGCCAGCCTG 
GCAACGGATG CCCTGGAAAC CCAAGAGCAG CGCCCCCAGC GACCACAAAC CCTAAATTTG 
AATGGTTGCC AGGCAGAAAG TTCCACGCCA TCTCAAGATG AAAATCCCGA AATTCAACAG 
GAAACTTTGC AGCCTACCGA AGTCAGGGAA GGAGCGGCAG GAGTAGGTGT TATACCCGGC 
GAGGATGACG AGGAAAGTCC TATCTACGAA GCAGTGGGCT ACAGCGATCC GCATGCCAAT 
CTGGGCAAGG TGCCACCCAT ATGGGTCCCG GATAACATGG CCGGGCAGTG CATGCAATGT 
CAGCAAAAGT TCACCATGAT CAAAAGGCGT CACCACTGTC GCGCCTGCGG AAAGGTCTTG 
TGCTCTGTCT GCTGTTCGCA ACGATTCCGA TTGGAATTCG CCACCGAACC CGAGTCTAGG 
GTTTGTGTGC AGTGTTATAT GATTCTCTCT GAGCGGCAGG CTAATGGATT GAATTCTGAG 
TCAGCACCGG GTTCGGCATT GCCGCCCACT CCCATTCGTT CGCCAAACCC AAACAATCCA 
ATGGAATATT GTTCTACAAT ACCACCACAC CGTCAGGTGG CCAACTCACC GGGTGCTCCG 
CCACCTAGTG TGATTGTGCC CGTGGGTGTG TTGAAAAAAA CCGATGGCAG CTCCTCCAAT 
TCCTCCAGTT CGGATGGCCA AAAGGGCCAA AGAAAGCGAA AGAGTGTTAT GTTCAGTGAT 
GGCATTGCGC CCGGCAGCGA ACTGGCCAGT ACCATGGAGC AGCAATGGGG CGAGTCCAAG 
CATTCTCGAA GGGGACTATC CAGGAGCGGC TCGGGAGCTG GTCAAAAGCC ACCAACGCCT 
GCGACAGAGG AGCCACCTCG CAGTATTGAC ACTAGCTCTA CATTGGGCCT AGTGGCACAG 
CTATTCCGCG GTTCCATTCC ACCAAGCGCA GCGGCTGCCA CATTATCGGC TACCGAGACA 
AGCGCTGGTC GCTCCTCATC CCAATCGCAG GCACAGACCT CGCCGCGTCG CAAAGCGGAA 
TCATCACGGA ATCGCAAACT ACCGCCAAAC GGAGATGCCC AAGGCTGCTA TCTGCCGCCG 
GAGGAGAACG GCCTGCCCCC AATTTGTATA AGCAGCAAAG AGGGCGGTGA GGTGGACTAC 
AAAGTAGTGC GCAACGATGG ATCACTCCTA GATCGATTGC AGAACGAAAC GCTCAAGTTC 
ATTATAAAGA ACAACTTCTT TGTGCTGGTC AAGATTGTTA ACATGAGCTG CTGTATGAAT 
CGATGGGTGC TGAACTTTAC GACATCCGGA CTACATCATA TGGGCAGCGA TGAGTTGATC 
ATTCTGCTGG AGATTAAGCA ACCCAAGCAA GAAGAATACG TGAAGGGGGA AGTCGCAATT 
CCGAAAGATT TGTTCCAGCA CCTGTACGAG ATCTACGTGG AGGCGGAGCA TGCCCGGTCC 
GGCACCCATG AGTTGGCTTT CACCAGTCCG CGAAATGCCA ATTTTCTGGG TTCCCGGGAG 
CATGGAGGAT TTATCTTTAT TCGGCCAACG TACCAGTGCT TGCAGGGAGT TATTGTACCA 
GACAATCCGT ATCTGGTTGG CGTGCTCATC CATAGACACG AGGTACCTTG GGCTAAGGTG 
CTACCTTTGC GGTTGATCCT CCGCTTGGGT GCCCAGTATC GGTACTATCC CTGTCCATTG 
ATCTCGGTGC GCGGCAGGGA GTCGGTGTAT GGCGAAATAG CTCAAACTAT CATTAACTTT 
CTGGTGGATT TTCGCAACTA TTCGTACTCT CTACCGGCCA TCCGAGGATT ATATATCCAC 
ATGGAGGATA GGCAGACGAC GGTGATTATT CCAAAGTACC GCCAGCAGGA TGTAATTAAG 
GCAATCAATA ATGCTAGTGA TCATATACTG GCTTTTGCTG GCAACTTCTC AAAAGTGGCC 
GATGGTCACT TGGTTTGCAT GCAGAATATC AACGACGATC GATCCGAGAT GTACTCCTAC 
TCCACGCAGG CGATTAATAT TCAGGGTCAA CCCAGGAAAA TCACTGGAGC TAGCTTCTTT 
GTGCTAAATG AAGCCCTCAA GAGCAGCAGT GGCTTGTCGG GTAAGTGCAG CATCGTGGAG 
GATGGCCTAA TGGTACAGAT TATGCCTGCC AAAATGGAGG AAGTGCGTCA GGCGTTGCGC 
AACCAAAACG ACATCGACAT CGTCTGTGGA CCCATTGACG CCACCGACGA TCAGAGTGAG 
ATTGTAAGCA TTAAGTGGGT GGACAATAAT CGTGACATCA ATGTGGGGGT TAAGTCGCCC 
ATTGATGACC AGCCCATGGA TGGCATTTCA AACATGCGAG TGCTGAAATG GGCCAGCTTT 
AACTATTCCA ATACCAACTA TGCCATCCGC CTGAGTGATA TCTACATTAT TAAGTGCGAG 
GACTGGTATG CAACAAATGG TGGAAACTGT GAGGAGATAA CACGGATTGC GGAGCAACTG 
GCTCGCAGTG CCTCGATGGC TCTGCTGCCA CTTCTCGATC TTTTAGCAGC TGCTGGCATT 
AATAAGCTTG GCCTAAGAGC CACGCTCGAT CAGGATAACG TGGGCTATGA AGCCGGAGCT 
CGTGGCTCCA AGCTACCGCC GTTGTACATG AACGCGCTGG ACAACCATCT GATAGCCACG 
CTGCATGGCG AATCCTCCGG CATTCAAGAG CCCATCATTC TAGAGCTGAT CTTTTACATA 
CTGAACGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sara cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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