wit cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wit
Entrez Gene ID	44096
Full Name	wishful thinking
Synonyms	1262/15,ALK3/BMPRII,BMP,CG10776,Dmel\CG10776,SE20,STK-D,Stk-D,WIT,Wit,Wit-C,l(3)64Aa,l(3)S126215,l(3)SH12
General protein information	Preferred Names wishful thinking Names CG10776 gene product from transcript CG10776-RA CG10776-PA CG10776-PB Serine/threonine kinase-D bone morphogenetic protein wit-PA wit-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64A5-64A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_079953.4	NP_524692.3	wishful thinking, isoform A
NM_001274466.1	NP_001261395.1	wishful thinking, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	wit	NP_524692.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005567	XP_315574.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data suggests that during oogenesis WIT acts as a type II receptor to regulate follicle cell patterning and morphogenesis of the eggshell
Title: The Drosophila BMPRII, wishful thinking, is required for eggshell patterning.

Results suggest that Wishful thinking binds multiple ligands to activate both bone morphogenetic protein and transforming growth factor-beta-like signaling pathways, and may mediate myoglianin-dependent signals in the nervous system.
Title: The Drosophila type II receptor, Wishful thinking, binds BMP and myoglianin to activate multiple TGFbeta family signaling pathways.

Wit globally regulates NMJ function by controlling both the growth and transmitter release properties of the synapse.
Title: Retrograde Gbb signaling through the Bmp type 2 receptor wishful thinking regulates systemic FMRFa expression in Drosophila.

The following wit gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wit cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19366	NM_001274466.1 Latest version!	Drosophila melanogaster wishful thinking (wit), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20459	NM_079953.4 Latest version!	Drosophila melanogaster wishful thinking (wit), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wit ( NM_001274466.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261395.1 Drosophila melanogaster wishful thinking (wit), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19366
Clone ID Related Accession (Same CDS sequence)	NM_001274466.1
Accession Version	NM_001274466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wishful thinking, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTGGG CTATCTATCT TTTGTTGGCC CTGATCTCGC TGGGCAGAGC AACACCTGTT 
CCCAATCGCC AGTATAGCTG TATGAGCTAC CAGGAGGACG ACAACTCATT TCACGATGAT 
GATGGCGATC AGGACTCTAG TGGCGAGCTG CAGGAGCAGC AGGTGGAGTC CACTCCGATT 
CCGAGCGAGC CACATAGACG CACCTGCCCA GATGGCTACA CTTTCTGTTT CACCATCTGG 
AACCAGACGG CCAATGGAGC GCGGGTTGTT AAACAGGGTT GCTGGAAGGA CAACACGGAT 
CGCACTTCCA TTTGCAGCCA GTCGGAATGT ACCAGCTCGG CTCCCACCTC GAAGACCAGT 
TCTTTGTACT ACTGTTGCTG TTCGGGAGGA GTGTGCAATG CCCAATATTC CGTGGTCGAA 
CCGGCACCTC TGGAACTGGG CTCCAATGAG GGACGGACTT CGATCACAAA TCGGGCGACA 
GAAAAACAGC ACCAGTCCTT TTTGGCCAGT ACAATGCTGG GCCTTGCCGG TGGACTCACA 
GCCCTCACAA TCGGCATCTT TCTGGCTGTT CAATATTGCC GCACGGCCAA GGAGAAGCCG 
GAACCGGAGG AGTCGCCTCT GGCACCATCG GGCCCTGGCT ACAGTTCTAA TCTCAGGAAT 
GTCGACAACA TGAATTTGAT CGGCATGCTG GGGAGCGGCA AGTATGGAAC TGTGATGAAG 
GGACTGCTCC ACGATCAGGA GGTGGCGGTT AAGATATATC CCGAAGAGCA TCATCAGTAC 
TATGTGAACG AGAGGAACAT CTATGCCTTG CCTTTGATGG AGTGTCCTGC GCTGTTGAGC 
TATTTCGGCT ACGATGAACG CTGTACCATG GACGGGCGAA TGGAGTATCA ACTGGTACTA 
TCACTGGCTC CCCTCGGATG CCTCCAGGAT TGGTTGATAG CCAATACCCT AACTTTCAGC 
GAATGCTGTG GAATGCTGCG GTCCATCACG CGTGGAATCT CGCACCTGCA CACGGAACTC 
CGACTGGGAG ATCAGCATAA GCCCTGTGTG GCTCATCGGG ATATCAACAC AAGGAATGTG 
TTGGTCCAAG CTGATCTCAG CTGTTGCATT GCGGACTTTG GTTTCGCTCT CAAGGTGTTT 
GGCTCCAAGT ACGAGTACAA GGGCGAGGTG GCCATGGCGG AAACGAAGAG CATCAATGAG 
GTGGGCACCC TTCGCTACAT GGCCCCAGAA TTACTGGAAG GAGCTGTAAA TCTGCGGGAT 
TGCGAAACCT CGCTGAAGCA AATGGATGTC TATGCCCTGG GCCTGGTGCT GTGGGAAGTG 
GCTACCCGCT GCTCGGATTT CTATGCCCCT GGTCAAGCAA CACCTCCCTA CAAAGCACCC 
TATGAGCAGG AGGTGGGCTC CCATCCCAGC TTTGATCAAA TGCAGGCGTT GGTGGTGCGG 
CACAAGGCGC GTCCCTTATT TCCCACCGGC TGGGGTGGAG GAGCAGCGGC TAAAGTGGTG 
CGGGATACTT GCGAGGACTG TTGGGATCAT GATGCGGATG CTAGGCTGAC CTCCCTGTGT 
GCCGAGGAGC GCATGCAGGA GATGTCGACT TTAAGGCCAC GAGCACAGGC TCAACCTTCC 
AGTCCACTGC TGAATACCAA CAATCTGGTG GCCAGTCCAA CTGCGGAACA GGGAATAAAT 
ATCATAGCCA CAACGACAAC GGCGGCTGCA GTCCATCACC AGATGAGCTC CGACACTACG 
GGACTTATAC AGCCACCTCC CAATCAGCAG ATACCTCTAG CTGCCTTGGA ACGGGAGAAG 
AATCATCTGA GCTACCCGCA ACAGCAATTG CAGCCGTATC AGGGCAGGAA TCCCTGCCAG 
GAGCGCAATC TGGCACCTCT GACAATGCGA ACTCCACCGG TTCTTGTGGA GCGGAGCAAG 
AAGCACAGTT TCCAGACACA GCCTCAGGAG AACTCACTGT CTTGCTTGGA GCATGATGTG 
AGTGTGGAGG AGCTGATAGC CAGCCATCAG CACCAGCAGC AAAAGAACAC GATTGTAAGC 
ACAGGTGGAA ATGGCAATTC CTGCTTGGGT CAGGGATTTC CCAAGCAGCA GAACACGGAT 
CAAAAACTCC GGGGATGGCA TGGAGTAAGA GCCCTGATCC ACAAGAAACT TTTTCGCAAA 
GAGCATGCCG AGGAGCTGTG TCGTCAGTTG CAGTTGGGCG AAGAGAAGTC CAATCTGGTG 
ACGGCTCTGC GTCGACCGAA CAACCTGGAT CTTAATCCTC GCCTCGATAA ACCTCCGCCG 
GATCAGCTGA GGAGTGCAGA GCAACGGATG GGAACTCCCG CTCACATTGT ACCCCGATCG 
CTGTCCAGCA GCCTGATCAA GCACATCAAC GGGACCACCA ACAATAACTC CATCCAGAGT 
CATGGTAGCG AATTGCAGAC CCTCACCCGT CCGGCTTCGA AGCGAAGACC AGGTCACCTG 
CGCACCAACT CCCTGATGGC CACCACGGGT CAGGGTCCGC CGACGGAGCA GCAAATGCGT 
CGTCAGCACA GTCTGGAGGT ATTCCGTGAG GTATTCAGCG GTCGGGGGAG CAGCGAGCGT 
TTGAGGGACC CCAGCGAGCG GGTCAAGACG CCGGGAGATG TGCCACCATC CGTACGGAAA 
GCCAGGGCCA GCAAGACACT GAGCTTGTAC GACGATCGGA TGATGGACTC CTCCCTGCTC 
AACATTCTCT AGCATGAGGA GGTTCTGCTG CTGGAGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261395.1
CDS	414..3155
Translation	MNWAIYLLLALISLGRATPVPNRQYSCMSYQEDDNSFHDDDGDQDSSGELQEQQVESTPIPSEPHRRTCPDGYTFCFTIWNQTANGARVVKQGCWKDNTDRTSICSQSECTSSAPTSKTSSLYYCCCSGGVCNAQYSVVEPAPLELGSNEGRTSITNRATEKQHQSFLASTMLGLAGGLTALTIGIFLAVQYCRTAKEKPEPEESPLAPSGPGYSSNLRNVDNMNLIGMLGSGKYGTVMKGLLHDQEVAVKIYPEEHHQYYVNERNIYALPLMECPALLSYFGYDERCTMDGRMEYQLVLSLAPLGCLQDWLIANTLTFSECCGMLRSITRGISHLHTELRLGDQHKPCVAHRDINTRNVLVQADLSCCIADFGFALKVFGSKYEYKGEVAMAETKSINEVGTLRYMAPELLEGAVNLRDCETSLKQMDVYALGLVLWEVATRCSDFYAPGQATPPYKAPYEQEVGSHPSFDQMQALVVRHKARPLFPTGWGGGAAAKVVRDTCEDCWDHDADARLTSLCAEERMQEMSTLRPRAQAQPSSPLLNTNNLVASPTAEQGINIIATTTTAAAVHHQMSSDTTGLIQPPPNQQIPLAALEREKNHLSYPQQQLQPYQGRNPCQERNLAPLTMRTPPVLVERSKKHSFQTQPQENSLSCLEHDVSVEELIASHQHQQQKNTIVSTGGNGNSCLGQGFPKQQNTDQKLRGWHGVRALIHKKLFRKEHAEELCRQLQLGEEKSNLVTALRRPNNLDLNPRLDKPPPDQLRSAEQRMGTPAHIVPRSLSSSLIKHINGTTNNNSIQSHGSELQTLTRPASKRRPGHLRTNSLMATTGQGPPTEQQMRRQHSLEVFREVFSGRGSSERLRDPSERVKTPGDVPPSVRKARASKTLSLYDDRMMDSSLLNIL*HEEVLLLEL

Target ORF information:

RefSeq Version	NM_001274466.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wishful thinking (wit), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274466.1

ORF Insert Sequence:

ATGAATTGGG CTATCTATCT TTTGTTGGCC CTGATCTCGC TGGGCAGAGC AACACCTGTT 
CCCAATCGCC AGTATAGCTG TATGAGCTAC CAGGAGGACG ACAACTCATT TCACGATGAT 
GATGGCGATC AGGACTCTAG TGGCGAGCTG CAGGAGCAGC AGGTGGAGTC CACTCCGATT 
CCGAGCGAGC CACATAGACG CACCTGCCCA GATGGCTACA CTTTCTGTTT CACCATCTGG 
AACCAGACGG CCAATGGAGC GCGGGTTGTT AAACAGGGTT GCTGGAAGGA CAACACGGAT 
CGCACTTCCA TTTGCAGCCA GTCGGAATGT ACCAGCTCGG CTCCCACCTC GAAGACCAGT 
TCTTTGTACT ACTGTTGCTG TTCGGGAGGA GTGTGCAATG CCCAATATTC CGTGGTCGAA 
CCGGCACCTC TGGAACTGGG CTCCAATGAG GGACGGACTT CGATCACAAA TCGGGCGACA 
GAAAAACAGC ACCAGTCCTT TTTGGCCAGT ACAATGCTGG GCCTTGCCGG TGGACTCACA 
GCCCTCACAA TCGGCATCTT TCTGGCTGTT CAATATTGCC GCACGGCCAA GGAGAAGCCG 
GAACCGGAGG AGTCGCCTCT GGCACCATCG GGCCCTGGCT ACAGTTCTAA TCTCAGGAAT 
GTCGACAACA TGAATTTGAT CGGCATGCTG GGGAGCGGCA AGTATGGAAC TGTGATGAAG 
GGACTGCTCC ACGATCAGGA GGTGGCGGTT AAGATATATC CCGAAGAGCA TCATCAGTAC 
TATGTGAACG AGAGGAACAT CTATGCCTTG CCTTTGATGG AGTGTCCTGC GCTGTTGAGC 
TATTTCGGCT ACGATGAACG CTGTACCATG GACGGGCGAA TGGAGTATCA ACTGGTACTA 
TCACTGGCTC CCCTCGGATG CCTCCAGGAT TGGTTGATAG CCAATACCCT AACTTTCAGC 
GAATGCTGTG GAATGCTGCG GTCCATCACG CGTGGAATCT CGCACCTGCA CACGGAACTC 
CGACTGGGAG ATCAGCATAA GCCCTGTGTG GCTCATCGGG ATATCAACAC AAGGAATGTG 
TTGGTCCAAG CTGATCTCAG CTGTTGCATT GCGGACTTTG GTTTCGCTCT CAAGGTGTTT 
GGCTCCAAGT ACGAGTACAA GGGCGAGGTG GCCATGGCGG AAACGAAGAG CATCAATGAG 
GTGGGCACCC TTCGCTACAT GGCCCCAGAA TTACTGGAAG GAGCTGTAAA TCTGCGGGAT 
TGCGAAACCT CGCTGAAGCA AATGGATGTC TATGCCCTGG GCCTGGTGCT GTGGGAAGTG 
GCTACCCGCT GCTCGGATTT CTATGCCCCT GGTCAAGCAA CACCTCCCTA CAAAGCACCC 
TATGAGCAGG AGGTGGGCTC CCATCCCAGC TTTGATCAAA TGCAGGCGTT GGTGGTGCGG 
CACAAGGCGC GTCCCTTATT TCCCACCGGC TGGGGTGGAG GAGCAGCGGC TAAAGTGGTG 
CGGGATACTT GCGAGGACTG TTGGGATCAT GATGCGGATG CTAGGCTGAC CTCCCTGTGT 
GCCGAGGAGC GCATGCAGGA GATGTCGACT TTAAGGCCAC GAGCACAGGC TCAACCTTCC 
AGTCCACTGC TGAATACCAA CAATCTGGTG GCCAGTCCAA CTGCGGAACA GGGAATAAAT 
ATCATAGCCA CAACGACAAC GGCGGCTGCA GTCCATCACC AGATGAGCTC CGACACTACG 
GGACTTATAC AGCCACCTCC CAATCAGCAG ATACCTCTAG CTGCCTTGGA ACGGGAGAAG 
AATCATCTGA GCTACCCGCA ACAGCAATTG CAGCCGTATC AGGGCAGGAA TCCCTGCCAG 
GAGCGCAATC TGGCACCTCT GACAATGCGA ACTCCACCGG TTCTTGTGGA GCGGAGCAAG 
AAGCACAGTT TCCAGACACA GCCTCAGGAG AACTCACTGT CTTGCTTGGA GCATGATGTG 
AGTGTGGAGG AGCTGATAGC CAGCCATCAG CACCAGCAGC AAAAGAACAC GATTGTAAGC 
ACAGGTGGAA ATGGCAATTC CTGCTTGGGT CAGGGATTTC CCAAGCAGCA GAACACGGAT 
CAAAAACTCC GGGGATGGCA TGGAGTAAGA GCCCTGATCC ACAAGAAACT TTTTCGCAAA 
GAGCATGCCG AGGAGCTGTG TCGTCAGTTG CAGTTGGGCG AAGAGAAGTC CAATCTGGTG 
ACGGCTCTGC GTCGACCGAA CAACCTGGAT CTTAATCCTC GCCTCGATAA ACCTCCGCCG 
GATCAGCTGA GGAGTGCAGA GCAACGGATG GGAACTCCCG CTCACATTGT ACCCCGATCG 
CTGTCCAGCA GCCTGATCAA GCACATCAAC GGGACCACCA ACAATAACTC CATCCAGAGT 
CATGGTAGCG AATTGCAGAC CCTCACCCGT CCGGCTTCGA AGCGAAGACC AGGTCACCTG 
CGCACCAACT CCCTGATGGC CACCACGGGT CAGGGTCCGC CGACGGAGCA GCAAATGCGT 
CGTCAGCACA GTCTGGAGGT ATTCCGTGAG GTATTCAGCG GTCGGGGGAG CAGCGAGCGT 
TTGAGGGACC CCAGCGAGCG GGTCAAGACG CCGGGAGATG TGCCACCATC CGTACGGAAA 
GCCAGGGCCA GCAAGACACT GAGCTTGTAC GACGATCGGA TGATGGACTC CTCCCTGCTC 
AACATTCTCT AGCATGAGGA GGTTCTGCTG CTGGAGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wit ( NM_079953.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524692.3 Drosophila melanogaster wishful thinking (wit), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa20459
Clone ID Related Accession (Same CDS sequence)	NM_079953.4
Accession Version	NM_079953.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wishful thinking, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079953.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTGGG CTATCTATCT TTTGTTGGCC CTGATCTCGC TGGGCAGAGC AACACCTGTT 
CCCAATCGCC AGTATAGCTG TATGAGCTAC CAGGAGGACG ACAACTCATT TCACGATGAT 
GATGGCGATC AGGACTCTAG TGGCGAGCTG CAGGAGCAGC AGGTGGAGTC CACTCCGATT 
CCGAGCGAGC CACATAGACG CACCTGCCCA GATGGCTACA CTTTCTGTTT CACCATCTGG 
AACCAGACGG CCAATGGAGC GCGGGTTGTT AAACAGGGTT GCTGGAAGGA CAACACGGAT 
CGCACTTCCA TTTGCAGCCA GTCGGAATGT ACCAGCTCGG CTCCCACCTC GAAGACCAGT 
TCTTTGTACT ACTGTTGCTG TTCGGGAGGA GTGTGCAATG CCCAATATTC CGTGGTCGAA 
CCGGCACCTC TGGAACTGGG CTCCAATGAG GGACGGACTT CGATCACAAA TCGGGCGACA 
GAAAAACAGC ACCAGTCCTT TTTGGCCAGT ACAATGCTGG GCCTTGCCGG TGGACTCACA 
GCCCTCACAA TCGGCATCTT TCTGGCTGTT CAATATTGCC GCACGGCCAA GGAGAAGCCG 
GAACCGGAGG AGTCGCCTCT GGCACCATCG GGCCCTGGCT ACAGTTCTAA TCTCAGGAAT 
GTCGACAACA TGAATTTGAT CGGCATGCTG GGGAGCGGCA AGTATGGAAC TGTGATGAAG 
GGACTGCTCC ACGATCAGGA GGTGGCGGTT AAGATATATC CCGAAGAGCA TCATCAGTAC 
TATGTGAACG AGAGGAACAT CTATGCCTTG CCTTTGATGG AGTGTCCTGC GCTGTTGAGC 
TATTTCGGCT ACGATGAACG CTGTACCATG GACGGGCGAA TGGAGTATCA ACTGGTACTA 
TCACTGGCTC CCCTCGGATG CCTCCAGGAT TGGTTGATAG CCAATACCCT AACTTTCAGC 
GAATGCTGTG GAATGCTGCG GTCCATCACG CGTGGAATCT CGCACCTGCA CACGGAACTC 
CGACTGGGAG ATCAGCATAA GCCCTGTGTG GCTCATCGGG ATATCAACAC AAGGAATGTG 
TTGGTCCAAG CTGATCTCAG CTGTTGCATT GCGGACTTTG GTTTCGCTCT CAAGGTGTTT 
GGCTCCAAGT ACGAGTACAA GGGCGAGGTG GCCATGGCGG AAACGAAGAG CATCAATGAG 
GTGGGCACCC TTCGCTACAT GGCCCCAGAA TTACTGGAAG GAGCTGTAAA TCTGCGGGAT 
TGCGAAACCT CGCTGAAGCA AATGGATGTC TATGCCCTGG GCCTGGTGCT GTGGGAAGTG 
GCTACCCGCT GCTCGGATTT CTATGCCCCT GGTCAAGCAA CACCTCCCTA CAAAGCACCC 
TATGAGCAGG AGGTGGGCTC CCATCCCAGC TTTGATCAAA TGCAGGCGTT GGTGGTGCGG 
CACAAGGCGC GTCCCTTATT TCCCACCGGC TGGGGTGGAG GAGCAGCGGC TAAAGTGGTG 
CGGGATACTT GCGAGGACTG TTGGGATCAT GATGCGGATG CTAGGCTGAC CTCCCTGTGT 
GCCGAGGAGC GCATGCAGGA GATGTCGACT TTAAGGCCAC GAGCACAGGC TCAACCTTCC 
AGTCCACTGC TGAATACCAA CAATCTGGTG GCCAGTCCAA CTGCGGAACA GGGAATAAAT 
ATCATAGCCA CAACGACAAC GGCGGCTGCA GTCCATCACC AGATGAGCTC CGACACTACG 
GGACTTATAC AGCCACCTCC CAATCAGCAG ATACCTCTAG CTGCCTTGGA ACGGGAGAAG 
AATCATCTGA GCTACCCGCA ACAGCAATTG CAGCCGTATC AGGGCAGGAA TCCCTGCCAG 
GAGCGCAATC TGGCACCTCT GACAATGCGA ACTCCACCGG TTCTTGTGGA GCGGAGCAAG 
AAGCACAGTT TCCAGACACA GCCTCAGGAG AACTCACTGT CTTGCTTGGA GCATGATGTG 
AGTGTGGAGG AGCTGATAGC CAGCCATCAG CACCAGCAGC AAAAGAACAC GATTGTAAGC 
ACAGGTGGAA ATGGCAATTC CTGCTTGGGT CAGGGATTTC CCAAGCAGCA GAACACGGAT 
CAAAAACTCC GGGGATGGCA TGGAGTAAGA GCCCTGATCC ACAAGAAACT TTTTCGCAAA 
GAGCATGCCG AGGAGCTGTG TCGTCAGTTG CAGTTGGGCG AAGAGAAGTC CAATCTGGTG 
ACGGCTCTGC GTCGACCGAA CAACCTGGAT CTTAATCCTC GCCTCGATAA ACCTCCGCCG 
GATCAGCTGA GGAGTGCAGA GCAACGGATG GGAACTCCCG CTCACATTGT ACCCCGATCG 
CTGTCCAGCA GCCTGATCAA GCACATCAAC GGGACCACCA ACAATAACTC CATCCAGAGT 
CATGGTAGCG AATTGCAGAC CCTCACCCGT CCGGCTTCGA AGCGAAGACC AGGTCACCTG 
CGCACCAACT CCCTGATGGC CACCACGGGT CAGGGTCCGC CGACGGAGCA GCAAATGCGT 
CGTCAGCACA GTCTGGAGGT ATTCCGTGAG GTATTCAGCG GTCGGGGGAG CAGCGAGCGT 
TTGAGGGACC CCAGCGAGCG GGTCAAGACG CCGGGAGATG TGCCACCATC CGTACGGAAA 
GCCAGGGCCA GCAAGACACT GAGCTTGTAC GACGATCGGA TGATGGACTC CTCCCTGCTC 
AACATTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524692.3
CDS	414..3125
Translation	MNWAIYLLLALISLGRATPVPNRQYSCMSYQEDDNSFHDDDGDQDSSGELQEQQVESTPIPSEPHRRTCPDGYTFCFTIWNQTANGARVVKQGCWKDNTDRTSICSQSECTSSAPTSKTSSLYYCCCSGGVCNAQYSVVEPAPLELGSNEGRTSITNRATEKQHQSFLASTMLGLAGGLTALTIGIFLAVQYCRTAKEKPEPEESPLAPSGPGYSSNLRNVDNMNLIGMLGSGKYGTVMKGLLHDQEVAVKIYPEEHHQYYVNERNIYALPLMECPALLSYFGYDERCTMDGRMEYQLVLSLAPLGCLQDWLIANTLTFSECCGMLRSITRGISHLHTELRLGDQHKPCVAHRDINTRNVLVQADLSCCIADFGFALKVFGSKYEYKGEVAMAETKSINEVGTLRYMAPELLEGAVNLRDCETSLKQMDVYALGLVLWEVATRCSDFYAPGQATPPYKAPYEQEVGSHPSFDQMQALVVRHKARPLFPTGWGGGAAAKVVRDTCEDCWDHDADARLTSLCAEERMQEMSTLRPRAQAQPSSPLLNTNNLVASPTAEQGINIIATTTTAAAVHHQMSSDTTGLIQPPPNQQIPLAALEREKNHLSYPQQQLQPYQGRNPCQERNLAPLTMRTPPVLVERSKKHSFQTQPQENSLSCLEHDVSVEELIASHQHQQQKNTIVSTGGNGNSCLGQGFPKQQNTDQKLRGWHGVRALIHKKLFRKEHAEELCRQLQLGEEKSNLVTALRRPNNLDLNPRLDKPPPDQLRSAEQRMGTPAHIVPRSLSSSLIKHINGTTNNNSIQSHGSELQTLTRPASKRRPGHLRTNSLMATTGQGPPTEQQMRRQHSLEVFREVFSGRGSSERLRDPSERVKTPGDVPPSVRKARASKTLSLYDDRMMDSSLLNIL

Target ORF information:

RefSeq Version	NM_079953.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wishful thinking (wit), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079953.4

ORF Insert Sequence:

ATGAATTGGG CTATCTATCT TTTGTTGGCC CTGATCTCGC TGGGCAGAGC AACACCTGTT 
CCCAATCGCC AGTATAGCTG TATGAGCTAC CAGGAGGACG ACAACTCATT TCACGATGAT 
GATGGCGATC AGGACTCTAG TGGCGAGCTG CAGGAGCAGC AGGTGGAGTC CACTCCGATT 
CCGAGCGAGC CACATAGACG CACCTGCCCA GATGGCTACA CTTTCTGTTT CACCATCTGG 
AACCAGACGG CCAATGGAGC GCGGGTTGTT AAACAGGGTT GCTGGAAGGA CAACACGGAT 
CGCACTTCCA TTTGCAGCCA GTCGGAATGT ACCAGCTCGG CTCCCACCTC GAAGACCAGT 
TCTTTGTACT ACTGTTGCTG TTCGGGAGGA GTGTGCAATG CCCAATATTC CGTGGTCGAA 
CCGGCACCTC TGGAACTGGG CTCCAATGAG GGACGGACTT CGATCACAAA TCGGGCGACA 
GAAAAACAGC ACCAGTCCTT TTTGGCCAGT ACAATGCTGG GCCTTGCCGG TGGACTCACA 
GCCCTCACAA TCGGCATCTT TCTGGCTGTT CAATATTGCC GCACGGCCAA GGAGAAGCCG 
GAACCGGAGG AGTCGCCTCT GGCACCATCG GGCCCTGGCT ACAGTTCTAA TCTCAGGAAT 
GTCGACAACA TGAATTTGAT CGGCATGCTG GGGAGCGGCA AGTATGGAAC TGTGATGAAG 
GGACTGCTCC ACGATCAGGA GGTGGCGGTT AAGATATATC CCGAAGAGCA TCATCAGTAC 
TATGTGAACG AGAGGAACAT CTATGCCTTG CCTTTGATGG AGTGTCCTGC GCTGTTGAGC 
TATTTCGGCT ACGATGAACG CTGTACCATG GACGGGCGAA TGGAGTATCA ACTGGTACTA 
TCACTGGCTC CCCTCGGATG CCTCCAGGAT TGGTTGATAG CCAATACCCT AACTTTCAGC 
GAATGCTGTG GAATGCTGCG GTCCATCACG CGTGGAATCT CGCACCTGCA CACGGAACTC 
CGACTGGGAG ATCAGCATAA GCCCTGTGTG GCTCATCGGG ATATCAACAC AAGGAATGTG 
TTGGTCCAAG CTGATCTCAG CTGTTGCATT GCGGACTTTG GTTTCGCTCT CAAGGTGTTT 
GGCTCCAAGT ACGAGTACAA GGGCGAGGTG GCCATGGCGG AAACGAAGAG CATCAATGAG 
GTGGGCACCC TTCGCTACAT GGCCCCAGAA TTACTGGAAG GAGCTGTAAA TCTGCGGGAT 
TGCGAAACCT CGCTGAAGCA AATGGATGTC TATGCCCTGG GCCTGGTGCT GTGGGAAGTG 
GCTACCCGCT GCTCGGATTT CTATGCCCCT GGTCAAGCAA CACCTCCCTA CAAAGCACCC 
TATGAGCAGG AGGTGGGCTC CCATCCCAGC TTTGATCAAA TGCAGGCGTT GGTGGTGCGG 
CACAAGGCGC GTCCCTTATT TCCCACCGGC TGGGGTGGAG GAGCAGCGGC TAAAGTGGTG 
CGGGATACTT GCGAGGACTG TTGGGATCAT GATGCGGATG CTAGGCTGAC CTCCCTGTGT 
GCCGAGGAGC GCATGCAGGA GATGTCGACT TTAAGGCCAC GAGCACAGGC TCAACCTTCC 
AGTCCACTGC TGAATACCAA CAATCTGGTG GCCAGTCCAA CTGCGGAACA GGGAATAAAT 
ATCATAGCCA CAACGACAAC GGCGGCTGCA GTCCATCACC AGATGAGCTC CGACACTACG 
GGACTTATAC AGCCACCTCC CAATCAGCAG ATACCTCTAG CTGCCTTGGA ACGGGAGAAG 
AATCATCTGA GCTACCCGCA ACAGCAATTG CAGCCGTATC AGGGCAGGAA TCCCTGCCAG 
GAGCGCAATC TGGCACCTCT GACAATGCGA ACTCCACCGG TTCTTGTGGA GCGGAGCAAG 
AAGCACAGTT TCCAGACACA GCCTCAGGAG AACTCACTGT CTTGCTTGGA GCATGATGTG 
AGTGTGGAGG AGCTGATAGC CAGCCATCAG CACCAGCAGC AAAAGAACAC GATTGTAAGC 
ACAGGTGGAA ATGGCAATTC CTGCTTGGGT CAGGGATTTC CCAAGCAGCA GAACACGGAT 
CAAAAACTCC GGGGATGGCA TGGAGTAAGA GCCCTGATCC ACAAGAAACT TTTTCGCAAA 
GAGCATGCCG AGGAGCTGTG TCGTCAGTTG CAGTTGGGCG AAGAGAAGTC CAATCTGGTG 
ACGGCTCTGC GTCGACCGAA CAACCTGGAT CTTAATCCTC GCCTCGATAA ACCTCCGCCG 
GATCAGCTGA GGAGTGCAGA GCAACGGATG GGAACTCCCG CTCACATTGT ACCCCGATCG 
CTGTCCAGCA GCCTGATCAA GCACATCAAC GGGACCACCA ACAATAACTC CATCCAGAGT 
CATGGTAGCG AATTGCAGAC CCTCACCCGT CCGGCTTCGA AGCGAAGACC AGGTCACCTG 
CGCACCAACT CCCTGATGGC CACCACGGGT CAGGGTCCGC CGACGGAGCA GCAAATGCGT 
CGTCAGCACA GTCTGGAGGT ATTCCGTGAG GTATTCAGCG GTCGGGGGAG CAGCGAGCGT 
TTGAGGGACC CCAGCGAGCG GGTCAAGACG CCGGGAGATG TGCCACCATC CGTACGGAAA 
GCCAGGGCCA GCAAGACACT GAGCTTGTAC GACGATCGGA TGATGGACTC CTCCCTGCTC 
AACATTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

wit cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報