cas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	cas
Entrez Gene ID	44018
Full Name	castor
Synonyms	CG2102,Cas,Dmel\CG2102,l(3)j1C2,l(3)neo33,ming
General protein information	Preferred Names castor Names CG2102 gene product from transcript CG2102-RC CG2102-PA CG2102-PC cas-PA cas-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83C1-83C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079938.4	NP_524677.1	castor, isoform A
NM_001275371.1	NP_001262300.1	castor, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression of castor protein is regulated by ABD-D protein in promoter region of castor gene.
Title: Sequence-specific interaction between ABD-B homeodomain and castor gene in Drosophila.

Castor (Cas) is a nuclear protein that is expressed in follicle stem cells and early follicle cell precursors and then is restricted to differentiated polar and stalk cells once egg chambers form
Title: Castor is required for Hedgehog-dependent cell-fate specification and follicle stem cell maintenance in Drosophila oogenesis.

a novel function for castor in regulating neuropeptide gene expression.
Title: Transcriptome analysis of Drosophila CNS midline cells reveals diverse peptidergic properties and a role for castor in neuronal differentiation.

show that the products of the genes klumpfuss, nab and castor play important roles in their specification via a genetic cascade
Title: A genetic cascade involving klumpfuss, nab and castor specifies the abdominal leucokinergic neurons in the Drosophila CNS.

Results suggest that the expression of cas is segment-specifically regulated negatively by Ubx and abdA genes, but positively by the AbdB gene.
Title: Drosophila castor is regulated negatively by the Ubx and abdA genes, but positively by the AbdB gene.

The following cas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06825	NM_079938.4 Latest version!	Drosophila melanogaster castor (cas), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa07767	NM_001275371.1 Latest version!	Drosophila melanogaster castor (cas), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cas ( NM_079938.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524677.1 Drosophila melanogaster castor (cas), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06825
Clone ID Related Accession (Same CDS sequence)	NM_079938.4
Accession Version	NM_079938.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	castor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079938.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACC AAATGGAGTT TATTATGCAA CTCTACATGA TGAACTTGAT GAAGCAGCAG 
CAGCAAATGC AGCTCCAGCT TCCGCAGCAG CAACAGCAGC AGCAGCTGGC TGGATACACT 
TACACACAGA ACGAGGATAT CTCTAGCAGC ACATCCGTGC AGCAGCAACA GCAGCAGCAG 
CAGGAGCAAC TGCAGCAGCC ACAGCCAGAT CTCCGCAAGA CAAGAACGCA CAAGCGCATT 
AGCTCGCAAA ACACCAACTG TAGTAGTAGC CGTAGTTGTA GTCCCAATAG TAATTTGATT 
GCTTTTCAAC CACAACAATA CCCAGGCGCT ACAGCGGCAA CTCCTTCCAC TCCCAACAGC 
CACCCCAGCA ACCTGGTCAA CCAGATGCTG CTCCAAAGCC TGCCGCCGCT GACGCAGCTC 
ATGTTGCAGC AGCAGCAGCA GCAACATTTG CTGACCACCT CCAACCTTCT ACTAACACCC 
ACCCACACCC CCAGTTCGCT GGGCAAGCAG GATCCACTGC AGCATCCGTT GTTGCTGGGC 
CAGTTTGCCG GCTCCGAACA GATGGCCACA AATAACTTCC TTCAATCATC CACAGTGACC 
TCCACGCCCA TCGAGAGAGA GAAGGCAGCC ACACCAGCAC CCTCCGCCGG AGCAACTGCC 
GGCAACCTTT CGGCGGCCCA GGTCAAGTTT GAGCAGGAGT CCGCCGACGA CGATGAGGAC 
GATGATAAGC CGCTGTCCAG CCTCACCAGC TGCAGCTCCT CGGGCCACAC CAACGCCAGC 
TCCGAGAAGC TGCTGTTGTC GGGCGTCCAT CCGCTGGAGT CAACCACCGA CAGCCTAGAC 
TCACCCAGCA TGTATACGCC CGTCAAGCAG CCGGCGGACT CATCGTACGG ACTCATCACA 
CCCGTCGACA GTGATCTGAC CCCCAACACA CCCCTCCAAC CGACCCAAAC AATATCCCTG 
CTGACGCCGC CGTCGAGTGA GCAGAGCAAG AGCCTGGTGA GCCTCTCGGC GGCCAGCGGC 
CTGGATGCTC TGCTCCAGAA CGAGGAGGTA CTGAAGAACC TGCGGAAAGT GTCCTCCTAC 
CTGGAGTGCG AGAACAGCCT GTGCAGGCAG GAGAACCTGC GGGAGCACTT CCATTGCCAC 
GAGGAGCCCT GCCAGGGCAA GATCCTGAGC AAGAAGGACG ACATCATCCG GCACCTGAAG 
TGGCACAAGA AGCGCAAGGA AAGCCTCAAG CTGGGCTTTG CCCGCTTCTC CTCCTCGGAC 
GACTGTGCAC CAGCCTACGG AGAGGGTTGC GCCTACAACT GGAAACAGAC CCACTACCAC 
TGCGTCTACG AGCACTGTCC CAAGGTGTAT GTGAGCACCA GTGACGTCCA GATGCATGCC 
AATTTCCACC GCAAGGACTC CGAGATCGTG AACGAGGGCT TCCGGCGCTT CCGGGCGCAC 
GAGACCTGCC GCATCGAGGA TTGCCCCTTT TTCGGCAAGA AGATCTCCCA CTACCACTGC 
TGTCGCGAGG GATGCACCCA CACCTTCAAG AACAAGGCCG ATATGGACAA GCACAAAACT 
TACCATCTGA AGGACCACCA GCTGAAGATG GACGGCTTCA AGAAGATCCT GAAGACCGAG 
GTATGTCCCT TCGACGCCTG CAAGTTCTCC ACCGTCTGCA ACCACATCCA CTGCGTCCGC 
GAGGGCTGCG ACTACATCCT GCACTCCAGC AGCCAGATGA TCAGCCACAA GAGAAAGCAC 
GATCGTCAGG ACGGAGAGCA GGCGTACCAG CAGTTTAAGA TCAAGCAGGA CGTGGAGGAG 
TCCTCCCTGG ATGCCATGCC CCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCCCACCAGC 
CTTAGTCAAT CCCAGAGCAG CAGCTCTGTA TGCGGCGGCA GCAACACCTC CACTCCACTC 
TCCTCTTTGT CCGCCGAGCA CTTCCTGGCT CGGAAACGCG GCAGGCCACC CAAGAAAATC 
CAACTGCCAG CCGACGCTCA GCAGTCGGAA GCCAAGCGCC TGAAAGTCGA AGACGAGTCC 
TCTAATCCCG CCATGCTCCT GCCCCAGTCC CAGCCAGCAG CCGCTGTGCA TCCGCTGACA 
AGTGGACTCT TTCCCGGTCT CCTGCCCGCT GCCGCCGCTC CTGGAGTGGA TCCCACAGCC 
CCTAACTTCC AGCTCACCCA CCTGATGGCC CTCTTCCAGT TGCAGAATCC CCTCTTCTAC 
CAGAACCTCT ATCCGGGAAT GACCCAGAAT TCCTCCATGC TCGGCAACCT GGCAGCACTT 
AGCGCCGCAT CGGCAGCAGC GGCGGCGGCA GCGGCGGCAA ATGGAGCCGG AGTCCAGCAG 
CCGAAGGCAG AGTTTAGCTT TAAGCCAGAG TTTAAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524677.1
CDS	194..2575
Translation	MSNQMEFIMQLYMMNLMKQQQQMQLQLPQQQQQQQLAGYTYTQNEDISSSTSVQQQQQQQQEQLQQPQPDLRKTRTHKRISSQNTNCSSSRSCSPNSNLIAFQPQQYPGATAATPSTPNSHPSNLVNQMLLQSLPPLTQLMLQQQQQQHLLTTSNLLLTPTHTPSSLGKQDPLQHPLLLGQFAGSEQMATNNFLQSSTVTSTPIEREKAATPAPSAGATAGNLSAAQVKFEQESADDDEDDDKPLSSLTSCSSSGHTNASSEKLLLSGVHPLESTTDSLDSPSMYTPVKQPADSSYGLITPVDSDLTPNTPLQPTQTISLLTPPSSEQSKSLVSLSAASGLDALLQNEEVLKNLRKVSSYLECENSLCRQENLREHFHCHEEPCQGKILSKKDDIIRHLKWHKKRKESLKLGFARFSSSDDCAPAYGEGCAYNWKQTHYHCVYEHCPKVYVSTSDVQMHANFHRKDSEIVNEGFRRFRAHETCRIEDCPFFGKKISHYHCCREGCTHTFKNKADMDKHKTYHLKDHQLKMDGFKKILKTEVCPFDACKFSTVCNHIHCVREGCDYILHSSSQMISHKRKHDRQDGEQAYQQFKIKQDVEESSLDAMPQQQQQQQQQQPTSLSQSQSSSSVCGGSNTSTPLSSLSAEHFLARKRGRPPKKIQLPADAQQSEAKRLKVEDESSNPAMLLPQSQPAAAVHPLTSGLFPGLLPAAAAPGVDPTAPNFQLTHLMALFQLQNPLFYQNLYPGMTQNSSMLGNLAALSAASAAAAAAAAANGAGVQQPKAEFSFKPEFKE

Target ORF information:

RefSeq Version	NM_079938.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster castor (cas), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079938.4

ORF Insert Sequence:

ATGTCCAACC AAATGGAGTT TATTATGCAA CTCTACATGA TGAACTTGAT GAAGCAGCAG 
CAGCAAATGC AGCTCCAGCT TCCGCAGCAG CAACAGCAGC AGCAGCTGGC TGGATACACT 
TACACACAGA ACGAGGATAT CTCTAGCAGC ACATCCGTGC AGCAGCAACA GCAGCAGCAG 
CAGGAGCAAC TGCAGCAGCC ACAGCCAGAT CTCCGCAAGA CAAGAACGCA CAAGCGCATT 
AGCTCGCAAA ACACCAACTG TAGTAGTAGC CGTAGTTGTA GTCCCAATAG TAATTTGATT 
GCTTTTCAAC CACAACAATA CCCAGGCGCT ACAGCGGCAA CTCCTTCCAC TCCCAACAGC 
CACCCCAGCA ACCTGGTCAA CCAGATGCTG CTCCAAAGCC TGCCGCCGCT GACGCAGCTC 
ATGTTGCAGC AGCAGCAGCA GCAACATTTG CTGACCACCT CCAACCTTCT ACTAACACCC 
ACCCACACCC CCAGTTCGCT GGGCAAGCAG GATCCACTGC AGCATCCGTT GTTGCTGGGC 
CAGTTTGCCG GCTCCGAACA GATGGCCACA AATAACTTCC TTCAATCATC CACAGTGACC 
TCCACGCCCA TCGAGAGAGA GAAGGCAGCC ACACCAGCAC CCTCCGCCGG AGCAACTGCC 
GGCAACCTTT CGGCGGCCCA GGTCAAGTTT GAGCAGGAGT CCGCCGACGA CGATGAGGAC 
GATGATAAGC CGCTGTCCAG CCTCACCAGC TGCAGCTCCT CGGGCCACAC CAACGCCAGC 
TCCGAGAAGC TGCTGTTGTC GGGCGTCCAT CCGCTGGAGT CAACCACCGA CAGCCTAGAC 
TCACCCAGCA TGTATACGCC CGTCAAGCAG CCGGCGGACT CATCGTACGG ACTCATCACA 
CCCGTCGACA GTGATCTGAC CCCCAACACA CCCCTCCAAC CGACCCAAAC AATATCCCTG 
CTGACGCCGC CGTCGAGTGA GCAGAGCAAG AGCCTGGTGA GCCTCTCGGC GGCCAGCGGC 
CTGGATGCTC TGCTCCAGAA CGAGGAGGTA CTGAAGAACC TGCGGAAAGT GTCCTCCTAC 
CTGGAGTGCG AGAACAGCCT GTGCAGGCAG GAGAACCTGC GGGAGCACTT CCATTGCCAC 
GAGGAGCCCT GCCAGGGCAA GATCCTGAGC AAGAAGGACG ACATCATCCG GCACCTGAAG 
TGGCACAAGA AGCGCAAGGA AAGCCTCAAG CTGGGCTTTG CCCGCTTCTC CTCCTCGGAC 
GACTGTGCAC CAGCCTACGG AGAGGGTTGC GCCTACAACT GGAAACAGAC CCACTACCAC 
TGCGTCTACG AGCACTGTCC CAAGGTGTAT GTGAGCACCA GTGACGTCCA GATGCATGCC 
AATTTCCACC GCAAGGACTC CGAGATCGTG AACGAGGGCT TCCGGCGCTT CCGGGCGCAC 
GAGACCTGCC GCATCGAGGA TTGCCCCTTT TTCGGCAAGA AGATCTCCCA CTACCACTGC 
TGTCGCGAGG GATGCACCCA CACCTTCAAG AACAAGGCCG ATATGGACAA GCACAAAACT 
TACCATCTGA AGGACCACCA GCTGAAGATG GACGGCTTCA AGAAGATCCT GAAGACCGAG 
GTATGTCCCT TCGACGCCTG CAAGTTCTCC ACCGTCTGCA ACCACATCCA CTGCGTCCGC 
GAGGGCTGCG ACTACATCCT GCACTCCAGC AGCCAGATGA TCAGCCACAA GAGAAAGCAC 
GATCGTCAGG ACGGAGAGCA GGCGTACCAG CAGTTTAAGA TCAAGCAGGA CGTGGAGGAG 
TCCTCCCTGG ATGCCATGCC CCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCCCACCAGC 
CTTAGTCAAT CCCAGAGCAG CAGCTCTGTA TGCGGCGGCA GCAACACCTC CACTCCACTC 
TCCTCTTTGT CCGCCGAGCA CTTCCTGGCT CGGAAACGCG GCAGGCCACC CAAGAAAATC 
CAACTGCCAG CCGACGCTCA GCAGTCGGAA GCCAAGCGCC TGAAAGTCGA AGACGAGTCC 
TCTAATCCCG CCATGCTCCT GCCCCAGTCC CAGCCAGCAG CCGCTGTGCA TCCGCTGACA 
AGTGGACTCT TTCCCGGTCT CCTGCCCGCT GCCGCCGCTC CTGGAGTGGA TCCCACAGCC 
CCTAACTTCC AGCTCACCCA CCTGATGGCC CTCTTCCAGT TGCAGAATCC CCTCTTCTAC 
CAGAACCTCT ATCCGGGAAT GACCCAGAAT TCCTCCATGC TCGGCAACCT GGCAGCACTT 
AGCGCCGCAT CGGCAGCAGC GGCGGCGGCA GCGGCGGCAA ATGGAGCCGG AGTCCAGCAG 
CCGAAGGCAG AGTTTAGCTT TAAGCCAGAG TTTAAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cas ( NM_001275371.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262300.1 Drosophila melanogaster castor (cas), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa07767
Clone ID Related Accession (Same CDS sequence)	NM_001275371.1
Accession Version	NM_001275371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	castor, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACC AAATGGAGTT TATTATGCAA CTCTACATGA TGAACTTGAT GAAGCAGCAG 
CAGCAAATGC AGCTCCAGCT TCCGCAGCAG CAACAGCAGC AGCAGCTGGC TGGATACACT 
TACACACAGA ACGAGGATAT CTCTAGCAGC ACATCCGTGC AGCAGCAACA GCAGCAGCAG 
CAGGAGCAAC TGCAGCAGCC ACAGCCAGAT CTCCGCAAGA CAAGAACGCA CAAGCGCATT 
AGCTCGCAAA ACACCAACTG TAGTAGTAGC CGTAGTTGTA GTCCCAATAT GACCTCCACG 
CCCATCGAGA GAGAGAAGGC AGCCACACCA GCACCCTCCG CCGGAGCAAC TGCCGGCAAC 
CTTTCGGCGG CCCAGGTCAA GTTTGAGCAG GAGTCCGCCG ACGACGATGA GGACGATGAT 
AAGCCGCTGT CCAGCCTCAC CAGCTGCAGC TCCTCGGGCC ACACCAACGC CAGCTCCGAG 
AAGCTGCTGT TGTCGGGCGT CCATCCGCTG GAGTCAACCA CCGACAGCCT AGACTCACCC 
AGCATGTATA CGCCCGTCAA GCAGCCGGCG GACTCATCGT ACGGACTCAT CACACCCGTC 
GACAGTGATC TGACCCCCAA CACACCCCTC CAACCGACCC AAACAATATC CCTGCTGACG 
CCGCCGTCGA GTGAGCAGAG CAAGAGCCTG GTGAGCCTCT CGGCGGCCAG CGGCCTGGAT 
GCTCTGCTCC AGAACGAGGA GGTACTGAAG AACCTGCGGA AAGTGTCCTC CTACCTGGAG 
TGCGAGAACA GCCTGTGCAG GCAGGAGAAC CTGCGGGAGC ACTTCCATTG CCACGAGGAG 
CCCTGCCAGG GCAAGATCCT GAGCAAGAAG GACGACATCA TCCGGCACCT GAAGTGGCAC 
AAGAAGCGCA AGGAAAGCCT CAAGCTGGGC TTTGCCCGCT TCTCCTCCTC GGACGACTGT 
GCACCAGCCT ACGGAGAGGG TTGCGCCTAC AACTGGAAAC AGACCCACTA CCACTGCGTC 
TACGAGCACT GTCCCAAGGT GTATGTGAGC ACCAGTGACG TCCAGATGCA TGCCAATTTC 
CACCGCAAGG ACTCCGAGAT CGTGAACGAG GGCTTCCGGC GCTTCCGGGC GCACGAGACC 
TGCCGCATCG AGGATTGCCC CTTTTTCGGC AAGAAGATCT CCCACTACCA CTGCTGTCGC 
GAGGGATGCA CCCACACCTT CAAGAACAAG GCCGATATGG ACAAGCACAA AACTTACCAT 
CTGAAGGACC ACCAGCTGAA GATGGACGGC TTCAAGAAGA TCCTGAAGAC CGAGGTATGT 
CCCTTCGACG CCTGCAAGTT CTCCACCGTC TGCAACCACA TCCACTGCGT CCGCGAGGGC 
TGCGACTACA TCCTGCACTC CAGCAGCCAG ATGATCAGCC ACAAGAGAAA GCACGATCGT 
CAGGACGGAG AGCAGGCGTA CCAGCAGTTT AAGATCAAGC AGGACGTGGA GGAGTCCTCC 
CTGGATGCCA TGCCCCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCCCAC CAGCCTTAGT 
CAATCCCAGA GCAGCAGCTC TGTATGCGGC GGCAGCAACA CCTCCACTCC ACTCTCCTCT 
TTGTCCGCCG AGCACTTCCT GGCTCGGAAA CGCGGCAGGC CACCCAAGAA AATCCAACTG 
CCAGCCGACG CTCAGCAGTC GGAAGCCAAG CGCCTGAAAG TCGAAGACGA GTCCTCTAAT 
CCCGCCATGC TCCTGCCCCA GTCCCAGCCA GCAGCCGCTG TGCATCCGCT GACAAGTGGA 
CTCTTTCCCG GTCTCCTGCC CGCTGCCGCC GCTCCTGGAG TGGATCCCAC AGCCCCTAAC 
TTCCAGCTCA CCCACCTGAT GGCCCTCTTC CAGTTGCAGA ATCCCCTCTT CTACCAGAAC 
CTCTATCCGG GAATGACCCA GAATTCCTCC ATGCTCGGCA ACCTGGCAGC ACTTAGCGCC 
GCATCGGCAG CAGCGGCGGC GGCAGCGGCG GCAAATGGAG CCGGAGTCCA GCAGCCGAAG 
GCAGAGTTTA GCTTTAAGCC AGAGTTTAAG GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262300.1
CDS	194..2269
Translation	MSNQMEFIMQLYMMNLMKQQQQMQLQLPQQQQQQQLAGYTYTQNEDISSSTSVQQQQQQQQEQLQQPQPDLRKTRTHKRISSQNTNCSSSRSCSPNMTSTPIEREKAATPAPSAGATAGNLSAAQVKFEQESADDDEDDDKPLSSLTSCSSSGHTNASSEKLLLSGVHPLESTTDSLDSPSMYTPVKQPADSSYGLITPVDSDLTPNTPLQPTQTISLLTPPSSEQSKSLVSLSAASGLDALLQNEEVLKNLRKVSSYLECENSLCRQENLREHFHCHEEPCQGKILSKKDDIIRHLKWHKKRKESLKLGFARFSSSDDCAPAYGEGCAYNWKQTHYHCVYEHCPKVYVSTSDVQMHANFHRKDSEIVNEGFRRFRAHETCRIEDCPFFGKKISHYHCCREGCTHTFKNKADMDKHKTYHLKDHQLKMDGFKKILKTEVCPFDACKFSTVCNHIHCVREGCDYILHSSSQMISHKRKHDRQDGEQAYQQFKIKQDVEESSLDAMPQQQQQQQQQQPTSLSQSQSSSSVCGGSNTSTPLSSLSAEHFLARKRGRPPKKIQLPADAQQSEAKRLKVEDESSNPAMLLPQSQPAAAVHPLTSGLFPGLLPAAAAPGVDPTAPNFQLTHLMALFQLQNPLFYQNLYPGMTQNSSMLGNLAALSAASAAAAAAAAANGAGVQQPKAEFSFKPEFKE

Target ORF information:

RefSeq Version	NM_001275371.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster castor (cas), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275371.1

ORF Insert Sequence:

ATGTCCAACC AAATGGAGTT TATTATGCAA CTCTACATGA TGAACTTGAT GAAGCAGCAG 
CAGCAAATGC AGCTCCAGCT TCCGCAGCAG CAACAGCAGC AGCAGCTGGC TGGATACACT 
TACACACAGA ACGAGGATAT CTCTAGCAGC ACATCCGTGC AGCAGCAACA GCAGCAGCAG 
CAGGAGCAAC TGCAGCAGCC ACAGCCAGAT CTCCGCAAGA CAAGAACGCA CAAGCGCATT 
AGCTCGCAAA ACACCAACTG TAGTAGTAGC CGTAGTTGTA GTCCCAATAT GACCTCCACG 
CCCATCGAGA GAGAGAAGGC AGCCACACCA GCACCCTCCG CCGGAGCAAC TGCCGGCAAC 
CTTTCGGCGG CCCAGGTCAA GTTTGAGCAG GAGTCCGCCG ACGACGATGA GGACGATGAT 
AAGCCGCTGT CCAGCCTCAC CAGCTGCAGC TCCTCGGGCC ACACCAACGC CAGCTCCGAG 
AAGCTGCTGT TGTCGGGCGT CCATCCGCTG GAGTCAACCA CCGACAGCCT AGACTCACCC 
AGCATGTATA CGCCCGTCAA GCAGCCGGCG GACTCATCGT ACGGACTCAT CACACCCGTC 
GACAGTGATC TGACCCCCAA CACACCCCTC CAACCGACCC AAACAATATC CCTGCTGACG 
CCGCCGTCGA GTGAGCAGAG CAAGAGCCTG GTGAGCCTCT CGGCGGCCAG CGGCCTGGAT 
GCTCTGCTCC AGAACGAGGA GGTACTGAAG AACCTGCGGA AAGTGTCCTC CTACCTGGAG 
TGCGAGAACA GCCTGTGCAG GCAGGAGAAC CTGCGGGAGC ACTTCCATTG CCACGAGGAG 
CCCTGCCAGG GCAAGATCCT GAGCAAGAAG GACGACATCA TCCGGCACCT GAAGTGGCAC 
AAGAAGCGCA AGGAAAGCCT CAAGCTGGGC TTTGCCCGCT TCTCCTCCTC GGACGACTGT 
GCACCAGCCT ACGGAGAGGG TTGCGCCTAC AACTGGAAAC AGACCCACTA CCACTGCGTC 
TACGAGCACT GTCCCAAGGT GTATGTGAGC ACCAGTGACG TCCAGATGCA TGCCAATTTC 
CACCGCAAGG ACTCCGAGAT CGTGAACGAG GGCTTCCGGC GCTTCCGGGC GCACGAGACC 
TGCCGCATCG AGGATTGCCC CTTTTTCGGC AAGAAGATCT CCCACTACCA CTGCTGTCGC 
GAGGGATGCA CCCACACCTT CAAGAACAAG GCCGATATGG ACAAGCACAA AACTTACCAT 
CTGAAGGACC ACCAGCTGAA GATGGACGGC TTCAAGAAGA TCCTGAAGAC CGAGGTATGT 
CCCTTCGACG CCTGCAAGTT CTCCACCGTC TGCAACCACA TCCACTGCGT CCGCGAGGGC 
TGCGACTACA TCCTGCACTC CAGCAGCCAG ATGATCAGCC ACAAGAGAAA GCACGATCGT 
CAGGACGGAG AGCAGGCGTA CCAGCAGTTT AAGATCAAGC AGGACGTGGA GGAGTCCTCC 
CTGGATGCCA TGCCCCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCCCAC CAGCCTTAGT 
CAATCCCAGA GCAGCAGCTC TGTATGCGGC GGCAGCAACA CCTCCACTCC ACTCTCCTCT 
TTGTCCGCCG AGCACTTCCT GGCTCGGAAA CGCGGCAGGC CACCCAAGAA AATCCAACTG 
CCAGCCGACG CTCAGCAGTC GGAAGCCAAG CGCCTGAAAG TCGAAGACGA GTCCTCTAAT 
CCCGCCATGC TCCTGCCCCA GTCCCAGCCA GCAGCCGCTG TGCATCCGCT GACAAGTGGA 
CTCTTTCCCG GTCTCCTGCC CGCTGCCGCC GCTCCTGGAG TGGATCCCAC AGCCCCTAAC 
TTCCAGCTCA CCCACCTGAT GGCCCTCTTC CAGTTGCAGA ATCCCCTCTT CTACCAGAAC 
CTCTATCCGG GAATGACCCA GAATTCCTCC ATGCTCGGCA ACCTGGCAGC ACTTAGCGCC 
GCATCGGCAG CAGCGGCGGC GGCAGCGGCG GCAAATGGAG CCGGAGTCCA GCAGCCGAAG 
GCAGAGTTTA GCTTTAAGCC AGAGTTTAAG GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Combined evidence annotation of transposable elements in genome sequences.
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cas cDNA ORF clone, Drosophila melanogaster(fruit fly)

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