Pten cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pten
Entrez Gene ID	43991
Full Name	Phosphatase and tensin homolog
Synonyms	CG5671,DPTEN,Dmel\CG5671,PTEN,PTEN3,dPTEN,dPten,dpten,pten
General protein information	Preferred Names Phosphatase and tensin homolog Names CG5671 gene product from transcript CG5671-RB CG5671-PA CG5671-PB CG5671-PC CG5671-PD CG5671-PE CG5671-PG CG5671-PH PI 3-phosphatase PTEN PtdIns(3,4,5)P3 phosphatase Pten-PA Pten-PB Pten-PC Pten-PD Pten-PE Pten-PG Pten-PH drosophila phosphatase and tensin homologue deleted on chromosome 10 phosphatase and tensin homolog phosphatase and tensin homologue phosphatidylinositol (3,4,5)-trisphosphate phosphatase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31B1-31B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_058076.4	NP_477424.1	phosphatase and tensin homolog, isoform C
NM_001169462.2	NP_001162933.1	phosphatase and tensin homolog, isoform G
NM_058074.4	NP_477422.2	phosphatase and tensin homolog, isoform H
NM_134321.3	NP_599148.1	phosphatase and tensin homolog, isoform E
NM_134322.3	NP_599149.1	phosphatase and tensin homolog, isoform D
NM_058075.4	NP_477423.1	phosphatase and tensin homolog, isoform B
NM_134320.3	NP_599147.1	phosphatase and tensin homolog, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pten	NP_032986.1
Gallus gallus (chicken)	PTEN	XP_421555.2
Drosophila melanogaster (fruit fly)	Pten	NP_001162933.1
	PTEN	XP_001102542.1
Bos taurus (cattle)	PTEN	XP_002698416.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009628	XP_553421.2
Pan troglodytes (chimpanzee)	PTEN	XP_521544.3
Danio rerio (zebrafish)	ptenb	NP_001001822.1
Homo sapiens (human)	PTEN	NP_000305.3
Rattus norvegicus (Norway rat)	Pten	NP_113794.1
Canis lupus familiaris (dog)	PTEN	NP_001003192.1
Xenopus tropicalis (tropical clawed frog)	pten	NP_001116943.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ectopic expression of dPTEN (a phosphatase and tensin homologue) in the fat body prolongs fruit fly life.
Title: Basic mechanisms of longevity: A case study of Drosophila pro-longevity genes.

PTEN deficient cells proliferate under conditions of nutrient restriction.
Title: Nutrient restriction enhances the proliferative potential of cells lacking the tumor suppressor PTEN in mitotic tissues.

loss of the tumor suppressor PTEN disrupts cell rearrangements by preventing the lengthening of newly formed junctions that become unstable and keep on rearranging
Title: PTEN controls junction lengthening and stability during cell rearrangement in epithelial tissue.

study demonstrates that in the Drosophila lymph gland the tumor suppressors TSC and PTEN control blood progenitor proliferation through a common TOR- and 4EBP-dependent pathway
Title: Multifaceted roles of PTEN and TSC orchestrate growth and differentiation of Drosophila blood progenitors.

Regeneration of Drosophila sensory neuron axons and dendrites is regulated by the Akt pathway involving Pten and microRNA bantam.
Title: Regeneration of Drosophila sensory neuron axons and dendrites is regulated by the Akt pathway involving Pten and microRNA bantam.

The following Pten gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pten cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15187	NM_001169462.2 Latest version!	Drosophila melanogaster phosphatase and tensin homolog, transcript variant G (Pten), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15187	NM_134322.3 Latest version!	Drosophila melanogaster phosphatase and tensin homolog, transcript variant D (Pten), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15187	NM_058075.4 Latest version!	Drosophila melanogaster phosphatase and tensin homolog, transcript variant B (Pten), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15187	NM_058074.4 Latest version!	Drosophila melanogaster phosphatase and tensin homolog, transcript variant H (Pten), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16411	NM_134321.3 Latest version!	Drosophila melanogaster phosphatase and tensin homolog, transcript variant E (Pten), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16411	NM_134320.3 Latest version!	Drosophila melanogaster phosphatase and tensin homolog, transcript variant A (Pten), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16441	NM_058076.4 Latest version!	Drosophila melanogaster phosphatase and tensin homolog, transcript variant C (Pten), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pten ( NM_001169462.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162933.1 Drosophila melanogaster phosphatase and tensin homolog, transcript variant G (Pten), mRNA.

Price & Availability↑

CloneID	OFa15187
Clone ID Related Accession (Same CDS sequence)	NM_001169462.2 , NM_058074.4 , NM_134322.3 , NM_058075.4
Accession Version	NM_001169462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatase and tensin homolog, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169462.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGGTGAA TCAACATACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162933.1
CDS	912..2456
Translation	MANTISLMSNVIRNVVSKKRIRYKEKGYDLDLTYINDNIIAMGYPAPDKLEGLFRNRLEDVFKLLEENHAQHYKIYNLCSERSYDVAKFRGRVAVYPFDDHNPPTIELIQRFCSDVDMWLKEDSSNVVAVHCKAGKGRTGTMICAYLVFSGIKKSADEALAWYDEKRTKDRKGVTIPSQRRYVQYFSKLVCSSVPYSKVSLNVCEIRFSESSCVQNLGMVECSISVLHDSATENAKPDRLKTLPIDFQKSFVLTIKPSIPVSGDVKFELTKKSPDKIICHFWLNTFFVRNYSPCESDGTVNKYIHTLSKSEIDDVHKDSEHKRFSEEFKISIVFEAENFSNDVQAEASEKERNENVLNFERSDYDSLSPNCYAEKKVLTAIVNDNTTKSQTIETLDHKDIVTKIQYDTSTNSKNTSTACKRKQPNSKTLLPSLNDSTKEEIKRNHIFNQPSIKKTDLIKWQNSEVHITSDTRSINENKNINYNSYITCKQSSPKFNCGTEDGEEDWESGESTYL

Target ORF information:

RefSeq Version	NM_001169462.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatase and tensin homolog, transcript variant G (Pten), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169462.2

ORF Insert Sequence:

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGGTGAA TCAACATACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pten ( NM_058074.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477422.2 Drosophila melanogaster phosphatase and tensin homolog, transcript variant H (Pten), mRNA.

Price & Availability↑

CloneID	OFa15187
Clone ID Related Accession (Same CDS sequence)	NM_001169462.2 , NM_058074.4 , NM_134322.3 , NM_058075.4
Accession Version	NM_058074.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatase and tensin homolog, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058074.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGGTGAA TCAACATACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477422.2
CDS	755..2299
Translation	MANTISLMSNVIRNVVSKKRIRYKEKGYDLDLTYINDNIIAMGYPAPDKLEGLFRNRLEDVFKLLEENHAQHYKIYNLCSERSYDVAKFRGRVAVYPFDDHNPPTIELIQRFCSDVDMWLKEDSSNVVAVHCKAGKGRTGTMICAYLVFSGIKKSADEALAWYDEKRTKDRKGVTIPSQRRYVQYFSKLVCSSVPYSKVSLNVCEIRFSESSCVQNLGMVECSISVLHDSATENAKPDRLKTLPIDFQKSFVLTIKPSIPVSGDVKFELTKKSPDKIICHFWLNTFFVRNYSPCESDGTVNKYIHTLSKSEIDDVHKDSEHKRFSEEFKISIVFEAENFSNDVQAEASEKERNENVLNFERSDYDSLSPNCYAEKKVLTAIVNDNTTKSQTIETLDHKDIVTKIQYDTSTNSKNTSTACKRKQPNSKTLLPSLNDSTKEEIKRNHIFNQPSIKKTDLIKWQNSEVHITSDTRSINENKNINYNSYITCKQSSPKFNCGTEDGEEDWESGESTYL

Target ORF information:

RefSeq Version	NM_058074.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatase and tensin homolog, transcript variant H (Pten), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058074.4

ORF Insert Sequence:

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGGTGAA TCAACATACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pten ( NM_134322.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599149.1 Drosophila melanogaster phosphatase and tensin homolog, transcript variant D (Pten), mRNA.

Price & Availability↑

CloneID	OFa15187
Clone ID Related Accession (Same CDS sequence)	NM_001169462.2 , NM_058074.4 , NM_134322.3 , NM_058075.4
Accession Version	NM_134322.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatase and tensin homolog, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134322.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGGTGAA TCAACATACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599149.1
CDS	413..1957
Translation	MANTISLMSNVIRNVVSKKRIRYKEKGYDLDLTYINDNIIAMGYPAPDKLEGLFRNRLEDVFKLLEENHAQHYKIYNLCSERSYDVAKFRGRVAVYPFDDHNPPTIELIQRFCSDVDMWLKEDSSNVVAVHCKAGKGRTGTMICAYLVFSGIKKSADEALAWYDEKRTKDRKGVTIPSQRRYVQYFSKLVCSSVPYSKVSLNVCEIRFSESSCVQNLGMVECSISVLHDSATENAKPDRLKTLPIDFQKSFVLTIKPSIPVSGDVKFELTKKSPDKIICHFWLNTFFVRNYSPCESDGTVNKYIHTLSKSEIDDVHKDSEHKRFSEEFKISIVFEAENFSNDVQAEASEKERNENVLNFERSDYDSLSPNCYAEKKVLTAIVNDNTTKSQTIETLDHKDIVTKIQYDTSTNSKNTSTACKRKQPNSKTLLPSLNDSTKEEIKRNHIFNQPSIKKTDLIKWQNSEVHITSDTRSINENKNINYNSYITCKQSSPKFNCGTEDGEEDWESGESTYL

Target ORF information:

RefSeq Version	NM_134322.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatase and tensin homolog, transcript variant D (Pten), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134322.3

ORF Insert Sequence:

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGGTGAA TCAACATACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pten ( NM_058075.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477423.1 Drosophila melanogaster phosphatase and tensin homolog, transcript variant B (Pten), mRNA.

Price & Availability↑

CloneID	OFa15187
Clone ID Related Accession (Same CDS sequence)	NM_001169462.2 , NM_058074.4 , NM_134322.3 , NM_058075.4
Accession Version	NM_058075.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatase and tensin homolog, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058075.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGGTGAA TCAACATACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477423.1
CDS	643..2187
Translation	MANTISLMSNVIRNVVSKKRIRYKEKGYDLDLTYINDNIIAMGYPAPDKLEGLFRNRLEDVFKLLEENHAQHYKIYNLCSERSYDVAKFRGRVAVYPFDDHNPPTIELIQRFCSDVDMWLKEDSSNVVAVHCKAGKGRTGTMICAYLVFSGIKKSADEALAWYDEKRTKDRKGVTIPSQRRYVQYFSKLVCSSVPYSKVSLNVCEIRFSESSCVQNLGMVECSISVLHDSATENAKPDRLKTLPIDFQKSFVLTIKPSIPVSGDVKFELTKKSPDKIICHFWLNTFFVRNYSPCESDGTVNKYIHTLSKSEIDDVHKDSEHKRFSEEFKISIVFEAENFSNDVQAEASEKERNENVLNFERSDYDSLSPNCYAEKKVLTAIVNDNTTKSQTIETLDHKDIVTKIQYDTSTNSKNTSTACKRKQPNSKTLLPSLNDSTKEEIKRNHIFNQPSIKKTDLIKWQNSEVHITSDTRSINENKNINYNSYITCKQSSPKFNCGTEDGEEDWESGESTYL

Target ORF information:

RefSeq Version	NM_058075.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatase and tensin homolog, transcript variant B (Pten), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058075.4

ORF Insert Sequence:

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGGTGAA TCAACATACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pten ( NM_134321.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599148.1 Drosophila melanogaster phosphatase and tensin homolog, transcript variant E (Pten), mRNA.

Price & Availability↑

CloneID	OFa16411
Clone ID Related Accession (Same CDS sequence)	NM_134321.3 , NM_134320.3
Accession Version	NM_134321.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatase and tensin homolog, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134321.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599148.1
CDS	413..1942
Translation	MANTISLMSNVIRNVVSKKRIRYKEKGYDLDLTYINDNIIAMGYPAPDKLEGLFRNRLEDVFKLLEENHAQHYKIYNLCSERSYDVAKFRGRVAVYPFDDHNPPTIELIQRFCSDVDMWLKEDSSNVVAVHCKAGKGRTGTMICAYLVFSGIKKSADEALAWYDEKRTKDRKGVTIPSQRRYVQYFSKLVCSSVPYSKVSLNVCEIRFSESSCVQNLGMVECSISVLHDSATENAKPDRLKTLPIDFQKSFVLTIKPSIPVSGDVKFELTKKSPDKIICHFWLNTFFVRNYSPCESDGTVNKYIHTLSKSEIDDVHKDSEHKRFSEEFKISIVFEAENFSNDVQAEASEKERNENVLNFERSDYDSLSPNCYAEKKVLTAIVNDNTTKSQTIETLDHKDIVTKIQYDTSTNSKNTSTACKRKQPNSKTLLPSLNDSTKEEIKRNHIFNQPSIKKTDLIKWQNSEVHITSDTRSINENKNINYNSYITCKQSSPKFNCGTEDGEEDWESE

Target ORF information:

RefSeq Version	NM_134321.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatase and tensin homolog, transcript variant E (Pten), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134321.3

ORF Insert Sequence:

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pten ( NM_134320.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599147.1 Drosophila melanogaster phosphatase and tensin homolog, transcript variant A (Pten), mRNA.

Price & Availability↑

CloneID	OFa16411
Clone ID Related Accession (Same CDS sequence)	NM_134321.3 , NM_134320.3
Accession Version	NM_134320.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatase and tensin homolog, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134320.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599147.1
CDS	643..2172
Translation	MANTISLMSNVIRNVVSKKRIRYKEKGYDLDLTYINDNIIAMGYPAPDKLEGLFRNRLEDVFKLLEENHAQHYKIYNLCSERSYDVAKFRGRVAVYPFDDHNPPTIELIQRFCSDVDMWLKEDSSNVVAVHCKAGKGRTGTMICAYLVFSGIKKSADEALAWYDEKRTKDRKGVTIPSQRRYVQYFSKLVCSSVPYSKVSLNVCEIRFSESSCVQNLGMVECSISVLHDSATENAKPDRLKTLPIDFQKSFVLTIKPSIPVSGDVKFELTKKSPDKIICHFWLNTFFVRNYSPCESDGTVNKYIHTLSKSEIDDVHKDSEHKRFSEEFKISIVFEAENFSNDVQAEASEKERNENVLNFERSDYDSLSPNCYAEKKVLTAIVNDNTTKSQTIETLDHKDIVTKIQYDTSTNSKNTSTACKRKQPNSKTLLPSLNDSTKEEIKRNHIFNQPSIKKTDLIKWQNSEVHITSDTRSINENKNINYNSYITCKQSSPKFNCGTEDGEEDWESE

Target ORF information:

RefSeq Version	NM_134320.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatase and tensin homolog, transcript variant A (Pten), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134320.3

ORF Insert Sequence:

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTACTAAAA TTCAATACGA CACATCAACC AATTCAAAAA ATACTTCGAC TGCGTGCAAA 
CGCAAACAGC CTAATAGCAA AACTCTTTTA CCAAGCTTGA ATGATTCCAC AAAAGAGGAA 
ATAAAACGAA ATCATATATT TAATCAACCG TCCATTAAGA AAACTGATTT AATCAAATGG 
CAGAATAGTG AAGTTCATAT CACCTCTGAC ACTCGAAGTA TAAACGAAAA TAAGAACATC 
AATTACAATT CATACATCAC ATGTAAACAA TCCTCTCCAA AATTCAACTG TGGTACCGAA 
GATGGCGAGG AAGATTGGGA ATCCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pten ( NM_058076.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477424.1 Drosophila melanogaster phosphatase and tensin homolog, transcript variant C (Pten), mRNA.

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CloneID	OFa16441
Clone ID Related Accession (Same CDS sequence)	NM_058076.4
Accession Version	NM_058076.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatase and tensin homolog, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058076.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTGTGAATC AACATACCTG TAATCAATGC TTATTACGGA CTTATAATTC TTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477424.1
CDS	413..1669
Translation	MANTISLMSNVIRNVVSKKRIRYKEKGYDLDLTYINDNIIAMGYPAPDKLEGLFRNRLEDVFKLLEENHAQHYKIYNLCSERSYDVAKFRGRVAVYPFDDHNPPTIELIQRFCSDVDMWLKEDSSNVVAVHCKAGKGRTGTMICAYLVFSGIKKSADEALAWYDEKRTKDRKGVTIPSQRRYVQYFSKLVCSSVPYSKVSLNVCEIRFSESSCVQNLGMVECSISVLHDSATENAKPDRLKTLPIDFQKSFVLTIKPSIPVSGDVKFELTKKSPDKIICHFWLNTFFVRNYSPCESDGTVNKYIHTLSKSEIDDVHKDSEHKRFSEEFKISIVFEAENFSNDVQAEASEKERNENVLNFERSDYDSLSPNCYAEKKVLTAIVNDNTTKSQTIETLDHKDIVVNQHTCNQCLLRTYNSW

Target ORF information:

RefSeq Version	NM_058076.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatase and tensin homolog, transcript variant C (Pten), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058076.4

ORF Insert Sequence:

ATGGCCAACA CTATTTCGTT AATGTCCAAC GTGATACGCA ATGTAGTGAG TAAAAAACGT 
ATACGATACA AAGAAAAAGG ATACGATTTG GATTTAACAT ACATTAATGA TAACATAATT 
GCCATGGGCT ATCCAGCACC GGATAAACTA GAGGGCCTAT TCAGAAACCG TCTGGAAGAT 
GTCTTTAAGC TATTGGAAGA GAATCATGCC CAGCATTACA AAATCTATAA CCTATGCTCG 
GAGCGTAGTT ACGACGTTGC TAAATTTCGC GGGAGAGTAG CTGTTTATCC GTTCGATGAT 
CATAACCCTC CAACGATTGA GTTAATACAA CGATTTTGCA GTGATGTTGA TATGTGGCTC 
AAGGAAGATT CGTCCAATGT TGTAGCCGTG CACTGTAAAG CTGGAAAGGG CAGAACCGGT 
ACCATGATCT GCGCATATTT AGTGTTCAGT GGAATTAAAA AATCCGCCGA CGAGGCATTA 
GCGTGGTATG ATGAGAAGCG TACAAAGGAC CGAAAAGGTG TGACAATCCC ATCTCAGCGT 
CGATATGTTC AGTATTTTTC CAAACTAGTT TGTTCAAGTG TTCCATATTC GAAAGTAAGC 
CTTAACGTAT GTGAAATACG CTTCTCGGAG TCCAGTTGTG TGCAAAACTT AGGCATGGTA 
GAATGTTCAA TATCTGTATT ACACGACTCA GCCACAGAAA ATGCAAAGCC AGATAGACTG 
AAAACCTTGC CGATTGATTT TCAAAAATCA TTTGTTTTGA CCATTAAACC ATCAATACCA 
GTTTCCGGCG ATGTAAAATT TGAACTCACA AAAAAATCCC CAGATAAAAT CATTTGTCAC 
TTTTGGCTAA ATACGTTTTT TGTACGAAAC TATTCACCTT GCGAGTCCGA TGGAACAGTT 
AACAAATATA TACACACTTT AAGCAAATCA GAAATCGATG ATGTACATAA AGACAGTGAA 
CATAAAAGAT TTTCAGAAGA ATTCAAGATT TCAATCGTTT TTGAGGCAGA GAATTTTAGC 
AATGATGTTC AAGCTGAAGC GTCCGAAAAA GAGAGAAACG AAAATGTTTT AAATTTCGAA 
AGATCAGACT ATGACAGTTT GTCTCCAAAC TGCTATGCCG AAAAAAAAGT CTTAACTGCG 
ATCGTCAATG ATAATACAAC GAAAAGTCAA ACAATAGAAA CGTTGGATCA TAAGGATATA 
GTTGTGAATC AACATACCTG TAATCAATGC TTATTACGGA CTTATAATTC TTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pten cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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