sbr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sbr
Entrez Gene ID	43944
Full Name	small bristles
Synonyms	CG1664,CG17335,CT4634,DNXF-1,Dm nxf1,DmNXF1,Dmel\CG1664,NFX1,NXF1,NXF1-p15,Nxf1,PABPr,dTap,l(1)9Fd,l(1)G1,l(1)ts,l(1)ts403,nxf1,sbt
General protein information	Preferred Names small bristles Names CG1664 gene product from transcript CG1664-RA CG1664-PA locus 1 nuclear export factor 1 sbr-PA shorter-bristles
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 9F4-9F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_079921.3	NP_524660.1	small bristles, isoform A
NM_001369982.1	NP_001356942.1	small bristles, isoform C
NM_001369981.1	NP_001356941.1	small bristles, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nxf1	NP_058093.2
Drosophila melanogaster (fruit fly)	sbr	NP_524660.1
Saccharomyces cerevisiae (baker's yeast)	MEX67	NP_015156.1
Rattus norvegicus (Norway rat)	Nxf1	NP_067590.1
Danio rerio (zebrafish)	nxf1	XP_001923961.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002193	XP_307993.5
Eremothecium gossypii	AGOS_AAL104C	NP_982438.1
Pan troglodytes (chimpanzee)	NXF1	XP_001157986.1
	NXF1	XP_001115982.1
Bos taurus (cattle)	NXF1	NP_001069023.1
Caenorhabditis elegans (roundworm)	nxf-1	NP_506567.1
Schizosaccharomyces pombe (fission yeast)	mex67	NP_595996.1
Xenopus tropicalis (tropical clawed frog)	nxf1	NP_001039200.1
Homo sapiens (human)	NXF1	NP_006353.2
Canis lupus familiaris (dog)	NXF1	XP_540901.4
Caenorhabditis elegans (roundworm)	nxf-2	NP_506568.2
Kluyveromyces lactis	KLLA0C09801g	XP_452635.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we show that the Dm NXF1 (SBR) protein is present not only in the nucleus but also in the cytoplasm
Title: Cytoplasmic localization of SBR (Dm NXF1) protein and its zonal distribution in the ganglia of Drosophila melanogaster larvae.

data indicate that origin recognition complex depletion affects the Nxf1 association with the mRNP complex.
Title: ORC interacts with THSC/TREX-2 and its subunits promote Nxf1 association with mRNP and mRNA export in Drosophila.

sbr12 deletion and short testis-specific isoforms of SBR cause male sterility.
Title: Testis-specific products of the Drosophila melanogaster sbr gene, encoding nuclear export factor 1, are necessary for male fertility.

For SBR protein we have shown the localization not only in the nucleus, but in the cytoplasm marking of characteristic cytoplasmic structures.
Title: [THE ROLE OF sbr/Dm nxf1 GENE DURING SYNCYTIAL PERIODS OF DEVELOPMENT IN DROSOPHILA MELANOGASTER].

Dm NXF1 (SBR) protein is present in considerable amounts at all stages of the spermatogenesis.
Title: [Spermatogenesis in Drosophila melanogaster: the role of the basic transport receptor of the mRNA (Dm NXF1)].

The following sbr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sbr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02294	NM_079921.3 Latest version!	Drosophila melanogaster small bristles (sbr), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa87672	NM_001369982.1 Latest version!	Drosophila melanogaster small bristles (sbr), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa87672	NM_001369981.1 Latest version!	Drosophila melanogaster small bristles (sbr), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sbr ( NM_079921.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524660.1 Drosophila melanogaster small bristles (sbr), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02294
Clone ID Related Accession (Same CDS sequence)	NM_079921.3
Accession Version	NM_079921.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	small bristles, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_079921.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCAAAC GCGGCGGTGG CAGTAGCCAG CGGTACAACA ACAACGTTGG AAATGGCGGC 
GGACGTTACA ACGCTCCCGA GGATTTCGAT GATTTTGATG TGGAGGATCG CCAGCGACGC 
AAGGATCGAA ACAAGCGGCG CGTCAGCTTT AAGCCCTCCC AATGTCTACA TAACAAAAAG 
GACATCAAGC TGCGACCCGA AGATTTGCGT CGATGGGACG AGGATGATGA CATGAGCGAC 
ATGACCACGG CCGTTAAGGA TAGACCCACC TCCCGACGTC GGGGATCGCC CATTCCGCGC 
GGCAAGTTCG GCAAACTGAT GCCCAACAGC TTTGGCTGGT ACCAAGTCAC GTTACAAAAC 
GCCCAGATAT ACGAAAAGGA AACACTCTTG AGTGCTCTAT TGGCAGCGAT GTCGCCACAT 
GTCTTTATTC CTCAATATTG GCGAGTGGAG CGAAACTGCG TAATCTTCTT TACGGACGAC 
TACGAGGCAG CCGAACGCAT TCAACATCTG GGCAAGAATG GCCATCTTCC AGATGGCTAT 
CGTCTGATGC CACGAGTACG CAGCGGTATA CCACTAGTGG CCATCGACGA TGCCTTCAAG 
GAGAAGATGA AGGTCACAAT GGCCAAGCGT TACAATATTC AAACCAAGGC GCTGGATCTT 
TCCCGTTTTC ATGCAGATCC GGATCTTAAG CAAGTTTTCT GCCCACTCTT TCGTCAGAAT 
GTGATGGGCG CTGCCATTGA CATTATGTGC GACAATATAC CCGATTTGGA GGCACTTAAC 
CTGAATGACA ACTCCATTAG CAGCATGGAG GCGTTTAAGG GTGTGGAGAA ACGCTTACCG 
AACCTCAAGA TTCTCTATTT GGGGGATAAC AAGATACCAT CTTTGGCCCA CCTTGTAGTG 
CTTCGCAATC TGTCCATCTT GGAACTCGTT TTAAAGAACA ATCCCTGCCG TTCCCGCTAC 
AAGGATTCCC AGCAGTTTAT CAGCGAAGTA CGTCGCAAGT TTCCCAAACT GGTTAAGTTG 
GACGGAGAGA CCCTGGAGCC GCAAATCACA TTTGATCTAT CCGAGCAGGG ACGTCTTCTC 
GAAACGAAGG CATCCTATCT GTGCGACGTC GCTGGTGCCG AGGTGGTGCG CCAGTTCCTG 
GACCAGTACT TCCGCATATT TGACTCGGGC AATCGGCAAG CTCTGCTAGA TGCCTACCAT 
GAGAAAGCGA TGCTCTCCAT ATCAATGCCT TCGGCCAGTC AGGCGGGCAG ATTGAACAGT 
TTCTGGAAGT TCAATCGCAA TCTCCGGCGC TTGTTAAACG GCGAAGAGAA TCGCACCCGA 
AACTTGAAGT ACGGACGCCT GGCATGTGTT TCCACATTGG ATGAATGGCC AAAAACGCAG 
CACGACCGAC GCACCTTCAC CGTCGACCTG ACCATCTACA ATACTTCAAT GATGGTTTTC 
ACCGTGACGG GATTATTCAA AGAGCTGAAC GACGAGACCA ACAATCCCGC CTCCATGGAA 
TTATATGACG TTCGCCACTT TGCCCGCACC TACGTGGTGG TGCCACAGAA TAATGGCTTT 
TGTATCCGCA ACGAGACGAT CTTCATCACA AACGCTACGC ACGAGCAGGT GCGAGAGTTC 
AAGCGATCGC AGCACCAGCC TGCTCCCGGA GCTATGCCCT CCACTTCCAG TGCAGTGACC 
AGTCCTCAGG CCGGGGCAGC GGCGGGTCTG CAGGGTCGTC TGAATGCGTT GGGCGTGGCC 
ACTGGACCGG TGGCTATACT ATCAGGAGAT CCGTTGGCGG CCACCGCACC GGTTAACAGC 
GGCAGTGCCG CCATATCGAC AACAGCAGTG GCACCTGGCG CCCAGGATGA GAGCACTAAA 
ATGCAAATGA TTGAAGCCAT GAGCGCCCAA AGCCAAATGA ATGTGATCTG GAGTCGGAAA 
TGCCTGGAGG AAACGAATTG GGACTTTAAC CATGCCGCCT TTGTGTTCGA GAAACTATTC 
AAGGAAAACA AAATACCGCC TGAGGCTTTT ATGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524660.1
CDS	505..2523
Translation	MPKRGGGSSQRYNNNVGNGGGRYNAPEDFDDFDVEDRQRRKDRNKRRVSFKPSQCLHNKKDIKLRPEDLRRWDEDDDMSDMTTAVKDRPTSRRRGSPIPRGKFGKLMPNSFGWYQVTLQNAQIYEKETLLSALLAAMSPHVFIPQYWRVERNCVIFFTDDYEAAERIQHLGKNGHLPDGYRLMPRVRSGIPLVAIDDAFKEKMKVTMAKRYNIQTKALDLSRFHADPDLKQVFCPLFRQNVMGAAIDIMCDNIPDLEALNLNDNSISSMEAFKGVEKRLPNLKILYLGDNKIPSLAHLVVLRNLSILELVLKNNPCRSRYKDSQQFISEVRRKFPKLVKLDGETLEPQITFDLSEQGRLLETKASYLCDVAGAEVVRQFLDQYFRIFDSGNRQALLDAYHEKAMLSISMPSASQAGRLNSFWKFNRNLRRLLNGEENRTRNLKYGRLACVSTLDEWPKTQHDRRTFTVDLTIYNTSMMVFTVTGLFKELNDETNNPASMELYDVRHFARTYVVVPQNNGFCIRNETIFITNATHEQVREFKRSQHQPAPGAMPSTSSAVTSPQAGAAAGLQGRLNALGVATGPVAILSGDPLAATAPVNSGSAAISTTAVAPGAQDESTKMQMIEAMSAQSQMNVIWSRKCLEETNWDFNHAAFVFEKLFKENKIPPEAFMK

Target ORF information:

RefSeq Version	NM_079921.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster small bristles (sbr), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079921.3

ORF Insert Sequence:

ATGCCCAAAC GCGGCGGTGG CAGTAGCCAG CGGTACAACA ACAACGTTGG AAATGGCGGC 
GGACGTTACA ACGCTCCCGA GGATTTCGAT GATTTTGATG TGGAGGATCG CCAGCGACGC 
AAGGATCGAA ACAAGCGGCG CGTCAGCTTT AAGCCCTCCC AATGTCTACA TAACAAAAAG 
GACATCAAGC TGCGACCCGA AGATTTGCGT CGATGGGACG AGGATGATGA CATGAGCGAC 
ATGACCACGG CCGTTAAGGA TAGACCCACC TCCCGACGTC GGGGATCGCC CATTCCGCGC 
GGCAAGTTCG GCAAACTGAT GCCCAACAGC TTTGGCTGGT ACCAAGTCAC GTTACAAAAC 
GCCCAGATAT ACGAAAAGGA AACACTCTTG AGTGCTCTAT TGGCAGCGAT GTCGCCACAT 
GTCTTTATTC CTCAATATTG GCGAGTGGAG CGAAACTGCG TAATCTTCTT TACGGACGAC 
TACGAGGCAG CCGAACGCAT TCAACATCTG GGCAAGAATG GCCATCTTCC AGATGGCTAT 
CGTCTGATGC CACGAGTACG CAGCGGTATA CCACTAGTGG CCATCGACGA TGCCTTCAAG 
GAGAAGATGA AGGTCACAAT GGCCAAGCGT TACAATATTC AAACCAAGGC GCTGGATCTT 
TCCCGTTTTC ATGCAGATCC GGATCTTAAG CAAGTTTTCT GCCCACTCTT TCGTCAGAAT 
GTGATGGGCG CTGCCATTGA CATTATGTGC GACAATATAC CCGATTTGGA GGCACTTAAC 
CTGAATGACA ACTCCATTAG CAGCATGGAG GCGTTTAAGG GTGTGGAGAA ACGCTTACCG 
AACCTCAAGA TTCTCTATTT GGGGGATAAC AAGATACCAT CTTTGGCCCA CCTTGTAGTG 
CTTCGCAATC TGTCCATCTT GGAACTCGTT TTAAAGAACA ATCCCTGCCG TTCCCGCTAC 
AAGGATTCCC AGCAGTTTAT CAGCGAAGTA CGTCGCAAGT TTCCCAAACT GGTTAAGTTG 
GACGGAGAGA CCCTGGAGCC GCAAATCACA TTTGATCTAT CCGAGCAGGG ACGTCTTCTC 
GAAACGAAGG CATCCTATCT GTGCGACGTC GCTGGTGCCG AGGTGGTGCG CCAGTTCCTG 
GACCAGTACT TCCGCATATT TGACTCGGGC AATCGGCAAG CTCTGCTAGA TGCCTACCAT 
GAGAAAGCGA TGCTCTCCAT ATCAATGCCT TCGGCCAGTC AGGCGGGCAG ATTGAACAGT 
TTCTGGAAGT TCAATCGCAA TCTCCGGCGC TTGTTAAACG GCGAAGAGAA TCGCACCCGA 
AACTTGAAGT ACGGACGCCT GGCATGTGTT TCCACATTGG ATGAATGGCC AAAAACGCAG 
CACGACCGAC GCACCTTCAC CGTCGACCTG ACCATCTACA ATACTTCAAT GATGGTTTTC 
ACCGTGACGG GATTATTCAA AGAGCTGAAC GACGAGACCA ACAATCCCGC CTCCATGGAA 
TTATATGACG TTCGCCACTT TGCCCGCACC TACGTGGTGG TGCCACAGAA TAATGGCTTT 
TGTATCCGCA ACGAGACGAT CTTCATCACA AACGCTACGC ACGAGCAGGT GCGAGAGTTC 
AAGCGATCGC AGCACCAGCC TGCTCCCGGA GCTATGCCCT CCACTTCCAG TGCAGTGACC 
AGTCCTCAGG CCGGGGCAGC GGCGGGTCTG CAGGGTCGTC TGAATGCGTT GGGCGTGGCC 
ACTGGACCGG TGGCTATACT ATCAGGAGAT CCGTTGGCGG CCACCGCACC GGTTAACAGC 
GGCAGTGCCG CCATATCGAC AACAGCAGTG GCACCTGGCG CCCAGGATGA GAGCACTAAA 
ATGCAAATGA TTGAAGCCAT GAGCGCCCAA AGCCAAATGA ATGTGATCTG GAGTCGGAAA 
TGCCTGGAGG AAACGAATTG GGACTTTAAC CATGCCGCCT TTGTGTTCGA GAAACTATTC 
AAGGAAAACA AAATACCGCC TGAGGCTTTT ATGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sbr ( NM_001369982.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356942.1 Drosophila melanogaster small bristles (sbr), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa87672
Clone ID Related Accession (Same CDS sequence)	NM_001369982.1 , NM_001369981.1
Accession Version	NM_001369982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	small bristles, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCCAC ATGTCTTTAT TCCTCAATAT TGGCGAGTGG AGCGAAACTG CGTAATCTTC 
TTTACGGACG ACTACGAGGC AGCCGAACGC ATTCAACATC TGGGCAAGAA TGGCCATCTT 
CCAGATGGCT ATCGTCTGAT GCCACGAGTA CGCAGCGGTA TACCACTAGT GGCCATCGAC 
GATGCCTTCA AGGAGAAGAT GAAGGTCACA ATGGCCAAGC GTTACAATAT TCAAACCAAG 
GCGCTGGATC TTTCCCGTTT TCATGCAGAT CCGGATCTTA AGCAAGTTTT CTGCCCACTC 
TTTCGTCAGA ATGTGATGGG CGCTGCCATT GACATTATGT GCGACAATAT ACCCGATTTG 
GAGGCACTTA ACCTGAATGA CAACTCCATT AGCAGCATGG AGGCGTTTAA GGGTGTGGAG 
AAACGCTTAC CGAACCTCAA GATTCTCTAT TTGGGGGATA ACAAGATACC ATCTTTGGCC 
CACCTTGTAG TGCTTCGCAA TCTGTCCATC TTGGAACTCG TTTTAAAGAA CAATCCCTGC 
CGTTCCCGCT ACAAGGATTC CCAGCAGTTT ATCAGCGAAG TACGTCGCAA GTTTCCCAAA 
CTGGTTAAGT TGGACGGAGA GACCCTGGAG CCGCAAATCA CATTTGATCT ATCCGAGCAG 
GGACGTCTTC TCGAAACGAA GGCATCCTAT CTGTGCGACG TCGCTGGTGC CGAGGTGGTG 
CGCCAGTTCC TGGACCAGTA CTTCCGCATA TTTGACTCGG GCAATCGGCA AGCTCTGCTA 
GATGCCTACC ATGAGAAAGC GATGCTCTCC ATATCAATGC CTTCGGCCAG TCAGGCGGGC 
AGATTGAACA GTTTCTGGAA GTTCAATCGC AATCTCCGGC GCTTGTTAAA CGGCGAAGAG 
AATCGCACCC GAAACTTGAA GTACGGACGC CTGGCATGTG TTTCCACATT GGATGAATGG 
CCAAAAACGC AGCACGACCG ACGCACCTTC ACCGTCGACC TGACCATCTA CAATACTTCA 
ATGATGGTTT TCACCGTGAC GGGATTATTC AAAGAGCTGA ACGACGAGAC CAACAATCCC 
GCCTCCATGG AATTATATGA CGTTCGCCAC TTTGCCCGCA CCTACGTGGT GGTGCCACAG 
AATAATGGCT TTTGTATCCG CAACGAGACG ATCTTCATCA CAAACGCTAC GCACGAGCAG 
GTGCGAGAGT TCAAGCGATC GCAGCACCAG CCTGCTCCCG GAGCTATGCC CTCCACTTCC 
AGTGCAGTGA CCAGTCCTCA GGCCGGGGCA GCGGCGGGTC TGCAGGGTCG TCTGAATGCG 
TTGGGCGTGG CCACTGGACC GGTGGCTATA CTATCAGGAG ATCCGTTGGC GGCCACCGCA 
CCGGTTAACA GCGGCAGTGC CGCCATATCG ACAACAGCAG TGGCACCTGG CGCCCAGGAT 
GAGAGCACTA AAATGCAAAT GATTGAAGCC ATGAGCGCCC AAAGCCAAAT GAATGTGATC 
TGGAGTCGGA AATGCCTGGA GGAAACGAAT TGGGACTTTA ACCATGCCGC CTTTGTGTTC 
GAGAAACTAT TCAAGGAAAA CAAAATACCG CCTGAGGCTT TTATGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356942.1
CDS	323..1933
Translation	MSPHVFIPQYWRVERNCVIFFTDDYEAAERIQHLGKNGHLPDGYRLMPRVRSGIPLVAIDDAFKEKMKVTMAKRYNIQTKALDLSRFHADPDLKQVFCPLFRQNVMGAAIDIMCDNIPDLEALNLNDNSISSMEAFKGVEKRLPNLKILYLGDNKIPSLAHLVVLRNLSILELVLKNNPCRSRYKDSQQFISEVRRKFPKLVKLDGETLEPQITFDLSEQGRLLETKASYLCDVAGAEVVRQFLDQYFRIFDSGNRQALLDAYHEKAMLSISMPSASQAGRLNSFWKFNRNLRRLLNGEENRTRNLKYGRLACVSTLDEWPKTQHDRRTFTVDLTIYNTSMMVFTVTGLFKELNDETNNPASMELYDVRHFARTYVVVPQNNGFCIRNETIFITNATHEQVREFKRSQHQPAPGAMPSTSSAVTSPQAGAAAGLQGRLNALGVATGPVAILSGDPLAATAPVNSGSAAISTTAVAPGAQDESTKMQMIEAMSAQSQMNVIWSRKCLEETNWDFNHAAFVFEKLFKENKIPPEAFMK

Target ORF information:

RefSeq Version	NM_001369982.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster small bristles (sbr), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369982.1

ORF Insert Sequence:

ATGTCGCCAC ATGTCTTTAT TCCTCAATAT TGGCGAGTGG AGCGAAACTG CGTAATCTTC 
TTTACGGACG ACTACGAGGC AGCCGAACGC ATTCAACATC TGGGCAAGAA TGGCCATCTT 
CCAGATGGCT ATCGTCTGAT GCCACGAGTA CGCAGCGGTA TACCACTAGT GGCCATCGAC 
GATGCCTTCA AGGAGAAGAT GAAGGTCACA ATGGCCAAGC GTTACAATAT TCAAACCAAG 
GCGCTGGATC TTTCCCGTTT TCATGCAGAT CCGGATCTTA AGCAAGTTTT CTGCCCACTC 
TTTCGTCAGA ATGTGATGGG CGCTGCCATT GACATTATGT GCGACAATAT ACCCGATTTG 
GAGGCACTTA ACCTGAATGA CAACTCCATT AGCAGCATGG AGGCGTTTAA GGGTGTGGAG 
AAACGCTTAC CGAACCTCAA GATTCTCTAT TTGGGGGATA ACAAGATACC ATCTTTGGCC 
CACCTTGTAG TGCTTCGCAA TCTGTCCATC TTGGAACTCG TTTTAAAGAA CAATCCCTGC 
CGTTCCCGCT ACAAGGATTC CCAGCAGTTT ATCAGCGAAG TACGTCGCAA GTTTCCCAAA 
CTGGTTAAGT TGGACGGAGA GACCCTGGAG CCGCAAATCA CATTTGATCT ATCCGAGCAG 
GGACGTCTTC TCGAAACGAA GGCATCCTAT CTGTGCGACG TCGCTGGTGC CGAGGTGGTG 
CGCCAGTTCC TGGACCAGTA CTTCCGCATA TTTGACTCGG GCAATCGGCA AGCTCTGCTA 
GATGCCTACC ATGAGAAAGC GATGCTCTCC ATATCAATGC CTTCGGCCAG TCAGGCGGGC 
AGATTGAACA GTTTCTGGAA GTTCAATCGC AATCTCCGGC GCTTGTTAAA CGGCGAAGAG 
AATCGCACCC GAAACTTGAA GTACGGACGC CTGGCATGTG TTTCCACATT GGATGAATGG 
CCAAAAACGC AGCACGACCG ACGCACCTTC ACCGTCGACC TGACCATCTA CAATACTTCA 
ATGATGGTTT TCACCGTGAC GGGATTATTC AAAGAGCTGA ACGACGAGAC CAACAATCCC 
GCCTCCATGG AATTATATGA CGTTCGCCAC TTTGCCCGCA CCTACGTGGT GGTGCCACAG 
AATAATGGCT TTTGTATCCG CAACGAGACG ATCTTCATCA CAAACGCTAC GCACGAGCAG 
GTGCGAGAGT TCAAGCGATC GCAGCACCAG CCTGCTCCCG GAGCTATGCC CTCCACTTCC 
AGTGCAGTGA CCAGTCCTCA GGCCGGGGCA GCGGCGGGTC TGCAGGGTCG TCTGAATGCG 
TTGGGCGTGG CCACTGGACC GGTGGCTATA CTATCAGGAG ATCCGTTGGC GGCCACCGCA 
CCGGTTAACA GCGGCAGTGC CGCCATATCG ACAACAGCAG TGGCACCTGG CGCCCAGGAT 
GAGAGCACTA AAATGCAAAT GATTGAAGCC ATGAGCGCCC AAAGCCAAAT GAATGTGATC 
TGGAGTCGGA AATGCCTGGA GGAAACGAAT TGGGACTTTA ACCATGCCGC CTTTGTGTTC 
GAGAAACTAT TCAAGGAAAA CAAAATACCG CCTGAGGCTT TTATGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sbr ( NM_001369981.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356941.1 Drosophila melanogaster small bristles (sbr), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa87672
Clone ID Related Accession (Same CDS sequence)	NM_001369982.1 , NM_001369981.1
Accession Version	NM_001369981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	small bristles, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCCAC ATGTCTTTAT TCCTCAATAT TGGCGAGTGG AGCGAAACTG CGTAATCTTC 
TTTACGGACG ACTACGAGGC AGCCGAACGC ATTCAACATC TGGGCAAGAA TGGCCATCTT 
CCAGATGGCT ATCGTCTGAT GCCACGAGTA CGCAGCGGTA TACCACTAGT GGCCATCGAC 
GATGCCTTCA AGGAGAAGAT GAAGGTCACA ATGGCCAAGC GTTACAATAT TCAAACCAAG 
GCGCTGGATC TTTCCCGTTT TCATGCAGAT CCGGATCTTA AGCAAGTTTT CTGCCCACTC 
TTTCGTCAGA ATGTGATGGG CGCTGCCATT GACATTATGT GCGACAATAT ACCCGATTTG 
GAGGCACTTA ACCTGAATGA CAACTCCATT AGCAGCATGG AGGCGTTTAA GGGTGTGGAG 
AAACGCTTAC CGAACCTCAA GATTCTCTAT TTGGGGGATA ACAAGATACC ATCTTTGGCC 
CACCTTGTAG TGCTTCGCAA TCTGTCCATC TTGGAACTCG TTTTAAAGAA CAATCCCTGC 
CGTTCCCGCT ACAAGGATTC CCAGCAGTTT ATCAGCGAAG TACGTCGCAA GTTTCCCAAA 
CTGGTTAAGT TGGACGGAGA GACCCTGGAG CCGCAAATCA CATTTGATCT ATCCGAGCAG 
GGACGTCTTC TCGAAACGAA GGCATCCTAT CTGTGCGACG TCGCTGGTGC CGAGGTGGTG 
CGCCAGTTCC TGGACCAGTA CTTCCGCATA TTTGACTCGG GCAATCGGCA AGCTCTGCTA 
GATGCCTACC ATGAGAAAGC GATGCTCTCC ATATCAATGC CTTCGGCCAG TCAGGCGGGC 
AGATTGAACA GTTTCTGGAA GTTCAATCGC AATCTCCGGC GCTTGTTAAA CGGCGAAGAG 
AATCGCACCC GAAACTTGAA GTACGGACGC CTGGCATGTG TTTCCACATT GGATGAATGG 
CCAAAAACGC AGCACGACCG ACGCACCTTC ACCGTCGACC TGACCATCTA CAATACTTCA 
ATGATGGTTT TCACCGTGAC GGGATTATTC AAAGAGCTGA ACGACGAGAC CAACAATCCC 
GCCTCCATGG AATTATATGA CGTTCGCCAC TTTGCCCGCA CCTACGTGGT GGTGCCACAG 
AATAATGGCT TTTGTATCCG CAACGAGACG ATCTTCATCA CAAACGCTAC GCACGAGCAG 
GTGCGAGAGT TCAAGCGATC GCAGCACCAG CCTGCTCCCG GAGCTATGCC CTCCACTTCC 
AGTGCAGTGA CCAGTCCTCA GGCCGGGGCA GCGGCGGGTC TGCAGGGTCG TCTGAATGCG 
TTGGGCGTGG CCACTGGACC GGTGGCTATA CTATCAGGAG ATCCGTTGGC GGCCACCGCA 
CCGGTTAACA GCGGCAGTGC CGCCATATCG ACAACAGCAG TGGCACCTGG CGCCCAGGAT 
GAGAGCACTA AAATGCAAAT GATTGAAGCC ATGAGCGCCC AAAGCCAAAT GAATGTGATC 
TGGAGTCGGA AATGCCTGGA GGAAACGAAT TGGGACTTTA ACCATGCCGC CTTTGTGTTC 
GAGAAACTAT TCAAGGAAAA CAAAATACCG CCTGAGGCTT TTATGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356941.1
CDS	86..1696
Translation	MSPHVFIPQYWRVERNCVIFFTDDYEAAERIQHLGKNGHLPDGYRLMPRVRSGIPLVAIDDAFKEKMKVTMAKRYNIQTKALDLSRFHADPDLKQVFCPLFRQNVMGAAIDIMCDNIPDLEALNLNDNSISSMEAFKGVEKRLPNLKILYLGDNKIPSLAHLVVLRNLSILELVLKNNPCRSRYKDSQQFISEVRRKFPKLVKLDGETLEPQITFDLSEQGRLLETKASYLCDVAGAEVVRQFLDQYFRIFDSGNRQALLDAYHEKAMLSISMPSASQAGRLNSFWKFNRNLRRLLNGEENRTRNLKYGRLACVSTLDEWPKTQHDRRTFTVDLTIYNTSMMVFTVTGLFKELNDETNNPASMELYDVRHFARTYVVVPQNNGFCIRNETIFITNATHEQVREFKRSQHQPAPGAMPSTSSAVTSPQAGAAAGLQGRLNALGVATGPVAILSGDPLAATAPVNSGSAAISTTAVAPGAQDESTKMQMIEAMSAQSQMNVIWSRKCLEETNWDFNHAAFVFEKLFKENKIPPEAFMK

Target ORF information:

RefSeq Version	NM_001369981.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster small bristles (sbr), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369981.1

ORF Insert Sequence:

ATGTCGCCAC ATGTCTTTAT TCCTCAATAT TGGCGAGTGG AGCGAAACTG CGTAATCTTC 
TTTACGGACG ACTACGAGGC AGCCGAACGC ATTCAACATC TGGGCAAGAA TGGCCATCTT 
CCAGATGGCT ATCGTCTGAT GCCACGAGTA CGCAGCGGTA TACCACTAGT GGCCATCGAC 
GATGCCTTCA AGGAGAAGAT GAAGGTCACA ATGGCCAAGC GTTACAATAT TCAAACCAAG 
GCGCTGGATC TTTCCCGTTT TCATGCAGAT CCGGATCTTA AGCAAGTTTT CTGCCCACTC 
TTTCGTCAGA ATGTGATGGG CGCTGCCATT GACATTATGT GCGACAATAT ACCCGATTTG 
GAGGCACTTA ACCTGAATGA CAACTCCATT AGCAGCATGG AGGCGTTTAA GGGTGTGGAG 
AAACGCTTAC CGAACCTCAA GATTCTCTAT TTGGGGGATA ACAAGATACC ATCTTTGGCC 
CACCTTGTAG TGCTTCGCAA TCTGTCCATC TTGGAACTCG TTTTAAAGAA CAATCCCTGC 
CGTTCCCGCT ACAAGGATTC CCAGCAGTTT ATCAGCGAAG TACGTCGCAA GTTTCCCAAA 
CTGGTTAAGT TGGACGGAGA GACCCTGGAG CCGCAAATCA CATTTGATCT ATCCGAGCAG 
GGACGTCTTC TCGAAACGAA GGCATCCTAT CTGTGCGACG TCGCTGGTGC CGAGGTGGTG 
CGCCAGTTCC TGGACCAGTA CTTCCGCATA TTTGACTCGG GCAATCGGCA AGCTCTGCTA 
GATGCCTACC ATGAGAAAGC GATGCTCTCC ATATCAATGC CTTCGGCCAG TCAGGCGGGC 
AGATTGAACA GTTTCTGGAA GTTCAATCGC AATCTCCGGC GCTTGTTAAA CGGCGAAGAG 
AATCGCACCC GAAACTTGAA GTACGGACGC CTGGCATGTG TTTCCACATT GGATGAATGG 
CCAAAAACGC AGCACGACCG ACGCACCTTC ACCGTCGACC TGACCATCTA CAATACTTCA 
ATGATGGTTT TCACCGTGAC GGGATTATTC AAAGAGCTGA ACGACGAGAC CAACAATCCC 
GCCTCCATGG AATTATATGA CGTTCGCCAC TTTGCCCGCA CCTACGTGGT GGTGCCACAG 
AATAATGGCT TTTGTATCCG CAACGAGACG ATCTTCATCA CAAACGCTAC GCACGAGCAG 
GTGCGAGAGT TCAAGCGATC GCAGCACCAG CCTGCTCCCG GAGCTATGCC CTCCACTTCC 
AGTGCAGTGA CCAGTCCTCA GGCCGGGGCA GCGGCGGGTC TGCAGGGTCG TCTGAATGCG 
TTGGGCGTGG CCACTGGACC GGTGGCTATA CTATCAGGAG ATCCGTTGGC GGCCACCGCA 
CCGGTTAACA GCGGCAGTGC CGCCATATCG ACAACAGCAG TGGCACCTGG CGCCCAGGAT 
GAGAGCACTA AAATGCAAAT GATTGAAGCC ATGAGCGCCC AAAGCCAAAT GAATGTGATC 
TGGAGTCGGA AATGCCTGGA GGAAACGAAT TGGGACTTTA ACCATGCCGC CTTTGTGTTC 
GAGAAACTAT TCAAGGAAAA CAAAATACCG CCTGAGGCTT TTATGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sbr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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