Rok cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rok
Entrez Gene ID	43916
Full Name	Rho kinase
Synonyms	CG9774,DROK,DRhk,DRok,Dmel\CG9774,Drok,ROCK,ROCK-1,ROCK1,ROCK2,ROK,ROKalpha/beta,Rhk,RhoK,Rock,Rok/ROCK,dROK,dRok,drok,rok
General protein information	Preferred Names Rho kinase Names CG9774 gene product from transcript CG9774-RC CG9774-PA CG9774-PB CG9774-PC CG9774-PD DRho-kinase ROCK Rho-associated kinase Rho-associated protein kinase Rho-kinase RhoA kinase Rok-PA Rok-PB Rok-PC Rok-PD drosophila rho-associated kinase rho kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14F2-14F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298408.1	NP_001285337.1	Rho kinase, isoform D
NM_001298407.1	NP_001285336.1	Rho kinase, isoform C
NM_001298406.1	NP_001285335.1	Rho kinase, isoform B
NM_080535.3	NP_536796.2	Rho kinase, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rock2	NP_033098.2
Drosophila melanogaster (fruit fly)	rok	NP_536796.2
Caenorhabditis elegans (roundworm)	let-502	NP_491440.1
Pan troglodytes (chimpanzee)	ROCK2	XP_525689.3
Rattus norvegicus (Norway rat)	Rock2	NP_037154.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000406	XP_310694.5
Xenopus tropicalis (tropical clawed frog)	rock2	XP_004914525.1
Homo sapiens (human)	ROCK2	NP_004841.2
	ROCK2	XP_001096931.2
Canis lupus familiaris (dog)	ROCK2	XP_540083.4
Danio rerio (zebrafish)	rock2a	NP_777288.1
Bos taurus (cattle)	ROCK2	NP_776877.1
Gallus gallus (chicken)	ROCK2	XP_004935820.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

polarity-induced spatiotemporal control of Rok and Pkn is important for unequal cortical expansion, ensuring correct cleavage furrow positioning and the establishment of physical asymmetry.
Title: Cell Polarity Regulates Biased Myosin Activity and Dynamics during Asymmetric Cell Division via Drosophila Rho Kinase and Protein Kinase N.

Loss of DRok function in tendon cells results in mis-orientation of tendon cell extensions and abnormal accumulation of Thrombospondin and betaPS-integrin, which may cause abnormal myotendinous junction formation and muscle morphogenesis.
Title: Establishment of the Muscle-Tendon Junction During Thorax Morphogenesis in Drosophila Requires the Rho-Kinase.

model in which Rok-induced phosphorylation of residues within the basic region mediates the activation of FHOD1 homolog Knittrig in controlling macrophage migration
Title: The Drosophila FHOD1-like formin Knittrig acts through Rok to promote stress fiber formation and directed macrophage migration during the cellular immune response.

Atonal and EGFR signalling orchestrate rok- and Drak-dependent adherens junction remodelling during ommatidia morphogenesis.
Title: Atonal and EGFR signalling orchestrate rok- and Drak-dependent adherens junction remodelling during ommatidia morphogenesis.

The structure of Shrm domain 2 (SD2), which mediates the interaction with Rock and is required for Shrm function, is reported.
Title: Structure of Shroom domain 2 reveals a three-segmented coiled-coil required for dimerization, Rock binding, and apical constriction.

The following Rok gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rok cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02293	NM_080535.3 Latest version!	Drosophila melanogaster Rho kinase (Rok), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa22201	NM_001298407.1 Latest version!	Drosophila melanogaster Rho kinase (Rok), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa22202	NM_001298406.1 Latest version!	Drosophila melanogaster Rho kinase (Rok), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa22203	NM_001298408.1 Latest version!	Drosophila melanogaster Rho kinase (Rok), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rok ( NM_080535.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_536796.2 Drosophila melanogaster Rho kinase (Rok), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02293
Clone ID Related Accession (Same CDS sequence)	NM_080535.3
Accession Version	NM_080535.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080535.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGCTG GACGAGAAAC TGTGACCAAG CAGCGCAGCA TGGATGTGGA ACGAAGGCGC 
CGGGCGAATA CGCTCGAGCG CGAGATGCGC GATCCGACCA GCATCTGCAA TGTGGACTGC 
CTGCTGGACA CGGTGTCGGC CTTGGTCAGC GATTGTGACC ACGAGTCCCT GAGGCGGCTT 
AAGAACATCG AGCAGTATGC GGCCAAATAC AAACCATTGG CAATGCAGAT CAATCAGCTG 
CGCATGAACG TCGAGGATTT CCATTTTATC AAACTCATCG GCGCCGGCGC CTTTGGAGAG 
GTGCAGCTGG TGCGGCACAA GTCCTCCAGC CAGGTGTACG CCATGAAGCG GCTGTCCAAA 
TTCGAGATGA TGAAGCGACC GGACTCCGCG TTCTTCTGGG AGGAGCGACA CATTATGGCG 
CACGCCAACT CCGAATGGAT CGTTCAGCTG CACTTTGCAT TTCAGGATGC CAAATACCTA 
TACATGGTGA TGGACTTTAT GCCCGGCGGC GATATAGTCT CGCTGATGGG TGACTACGAC 
ATCCCGGAGA AGTGGGCCAT CTTTTACACA ATGGAGGTGG TTCTCGCGCT GGACACCATT 
CACAATATGG GCTTCGTGCA TCGGGACGTT AAGCCGGATA ATATGCTCCT CGACAGCTAT 
GGCCACCTGA AATTGGCCGA CTTTGGCACC TGTATGCGAA TGGGCGCCAA TGGCCAAGTG 
GTGTCCAGCA ATGCCGTTGG CACGCCGGAC TACATCAGCC CGGAGGTGCT GCAGTCGCAG 
GGCGTGGACA ACGAGTACGG GCGGGAATGC GACTGGTGGT CGGTGGGCAT TTTCCTCTAC 
GAGATGCTGT TCGGCGAGAC ACCATTCTAC GCAGACTCGC TGGTGGGCAC CTACGGCAAG 
ATCATGGACC ACAAGAACTC GCTTAGCTTT CCACCCGAGG TTGAGATCAG CGAACAGGCG 
AAGGCACTGA TCCGCGCCTT TCTCACGGAC CGAACGCAAC GTTTGGGTCG CTACGGCATC 
GAGGACATTA AGGCGCATCC GTTTTTCCGA AATGATACCT GGTCGTTCGA CAACATTCGC 
GAGAGTGTGC CGCCTGTGGT GCCCGAACTC TCCTCCGACG ATGATACGCG AAATTTCGAG 
GACATTGAGC GCGATGAGAA GCCGGAGGAA GTGTTTCCCG TACCCAAGGG CTTCGATGGC 
AATCACTTGC CCTTCATCGG CTTTACGTAT ACGGGTGACT ATCAGCTGCT GTCTAGCGAC 
ACCGTGGATG CGGAGTCGAA GGAGGCCAAT GTGGCAAACA GCGGGGCGGC CAGCAATAAT 
CACGGCCACG GTCATAATCA CCGCCATCGG CCGTCGAATT CCAACGAGCT GAAGCGCCTG 
GAGGCGCTGC TCGAGCGGGA GCGTGGCAGG TCGGAAGCCT TGGAGCAGCA GGATGCTGGA 
CTGCGGCAGC AGATTGAGCT GATAACAAAA CGCGAAGCGG AGCTGCAGAG GATTGCCTCA 
GAGTACGAGA AGGATCTGGC TTTGCGTCAA CACAACTACA AGGTGGCCAT GCAGAAGGTG 
GAGCAGGAGA TCGAGCTGCG CAAGAAGACG GAGGCACTGC TCGTGGAAAC GCAGCGCAAT 
CTGGAAAACG AGCAAAAGAC GCGGGCACGC GACCTCAATA TCAACGACAA GGTGGTCTCA 
CTCGAAAAGC AGCTGCTTGA GATGGAGCAG AGCTACAAAA CGGAGACGGA GAACACTCAA 
AAGCTGAAAA AGCACAATGC CGAGCTGGAC TTCACGGTCA AGTCGCAGGA GGAGAAGGTG 
CGCGACATGG TCGACATGAT CGACACGCTA CAAAAGCATA AGGAGGAGCT CGGCCAGGAG 
AATGCCGAGC TGCAGGCGCT GGTGGTGCAG GAGAAGAATC TGCGATCGCA GCTCAAGGAG 
ATGCACAAGG AGGCCGAGAA TAAGATGCAG ACGCTGATCA ACGACATTGA ACGTACTATG 
TGTCGCGAAC AAAAGGCGCA AGAGGACAAC CGGGCGTTGC TTGAGAAGAT CAGCGACTTG 
GAGAAGGCGC ATGCCGGCCT CGACTTCGAA CTGAAGGCGG CCCAGGGCCG CTATCAGCAG 
GAGGTCAAGG CACACCAAGA GACGGAGAAG TCGCGGCTCG TCTCCCGCGA GGAGGCCAAT 
CTGCAGGAGG TAAAGGCGCT GCAGTCCAAG CTCAACGAGG AGAAGTCCGC TCGCATCAAG 
GCCGATCAGC ATTCTCAGGA AAAAGAGCGT CAGCTGAGCA TGCTGTCCGT GGACTATCGC 
CAAATCCAAC TACGTCTCCA GAAGCTCGAA GGCGAGTGCC GCCAGGAGTC GGAGAAGGTG 
GCCGCTCTGC AGTCACAGCT CGACCAGGAG CACAGCAAAC GCAATGCCTT GCTATCGGAA 
CTCAGCCTGC ACAGCTCGGA GGTGGCCCAC CTGCGATCCC GCGAAAACCA GCTACAGAAG 
GAACTGTCTA CCCAGCGCGA GGCGAAGCGA CGCTTCGAGG AGGACCTCAC CCAGCTAAAG 
AGCACACACC ACGAAGCGCT GGCCAATAAC CGTGAGCTTC AGGCCCAACT CGAGGCGGAG 
CAGTGCTTCT CGCGGCTGTA CAAGACACAG GCGAACGAGA ATCGCGAAGA GAGCGCTGAG 
CGGCTGTCGA AGATCGAGGA TCTTGAGGAG GAACGCGTCT CACTCAAGCA CCAGGTACAG 
GTGGCCGTTG CCCGTGCAGA CTCAGAGGCT TTGGCCCGAT CCATTGCCGA AGAGACGGTG 
GCGGATCTCG AAAAGGAGAA GACTATCAAG GAGCTAGAGC TGAAGGACTT CGTCATGAAG 
CATCGCAACG AGATCAACGC CAAGGAGGCG GCCCTAGCCA CGCTCAAGGA GGCCGAAAAC 
GAACTGCACA AGAAGCTGGG CCAGAAGGCA GCCGAGTACG AGGATCTTGT CCAGCAGCAC 
AAGAAGCAGC AGGAGGAGCT TGCGCTGATG AGAAGCAGCA AGGACGAAGA GATCACCAAG 
CTGCTGGATA AGTGCAAGAA CGAAGTGCTT CTCAAACAGG TGGCAGTGAA CAAGTTGGCC 
GAAGTGATGA ATCGTCGCGA CTCCGACCTG CCCAAACAAA AGAACAAGGC GCGCTCGACA 
GCCGAGCTGC GCAAAAAGGA GAAGGAGATG CGTCGGCTGC AGCAGGAGCT CTCGCAGGAG 
CGCGACAAGT TCAACCAGCT GCTGCTCAAG CACCAGGACC TGCAGCAGCT GTGCGCCGAG 
GAGCAGCAGC TCAAGCAGAA GATGGTCATG GAGATCGATT GCAAGGCCAC CGAGATCGAG 
AACCTGCAGA GCAAGCTCAA CGAGACGGCG TCGCTCTCCT CGGCGGACAA CGATCCCGAG 
GATAGCCAGC ACTCCTCTCT GCTCTCCCTC ACACAGGACT CGGTCTTCGA GGGCTGGCTA 
AGTGTGCCCA ATAAGCAGAA TCGGCGACGT GGCCACGGCT GGAAGCGGCA GTACGTCATT 
GTGTCGTCGC GCAAAATCAT CTTCTACAAT TCGGACATTG ACAAGCACAA TACCACGGAT 
GCCGTGCTCA TCCTGGACCT GAGCAAGGTG TACCACGTTC GTAGCGTCAC TCAGGGCGAT 
GTCATACGCG CCGATGCCAA AGAGATTCCA CGCATATTCC AGCTACTGTA CGCCGGCGAG 
GGCGCCTCCC ATCGTCCCGA CGAGCAGAGC CAGTTGGATG TGAGCGTGCT GCACGGTAAT 
TGCAACGAGG AGCGCCCGGG CACCATTGTC CACAAAGGTC ACGAGTTCGT TCACATTACC 
TACCACATGC CCACGGCTTG CGAGGTGTGC CCGAAGCCTT TGTGGCACAT GTTCAAGCCG 
CCGGCGGCTT ACGAATGCAA GAGATGCCGC AACAAGATAC ACAAGGAGCA TGTGGACAAA 
CACGACCCGC TGGCGCCCTG TAAGCTCAAT CACGATCCGC GCAGCGCCCG TGACATGCTG 
CTGCTGGCCG CCACGCCCGA GGATCAATCG CTGTGGGTGG CGCGCCTGCT GAAGCGTATC 
CAAAAGAGTG GATATAAGGC GGCTTCGTAC AACAACAACA GCACCGATGG CAGCAAGATA 
TCGCCCAGCC AATCGACGCG CTCCTCCTAC AAGCCGTATG CGGTTAATGT CCAACGCTCC 
GCCACGCTGC CAGCCAATTC ATCGCTGAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536796.2
CDS	1066..5238
Translation	MPAGRETVTKQRSMDVERRRRANTLEREMRDPTSICNVDCLLDTVSALVSDCDHESLRRLKNIEQYAAKYKPLAMQINQLRMNVEDFHFIKLIGAGAFGEVQLVRHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQLHFAFQDAKYLYMVMDFMPGGDIVSLMGDYDIPEKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNMLLDSYGHLKLADFGTCMRMGANGQVVSSNAVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLYEMLFGETPFYADSLVGTYGKIMDHKNSLSFPPEVEISEQAKALIRAFLTDRTQRLGRYGIEDIKAHPFFRNDTWSFDNIRESVPPVVPELSSDDDTRNFEDIERDEKPEEVFPVPKGFDGNHLPFIGFTYTGDYQLLSSDTVDAESKEANVANSGAASNNHGHGHNHRHRPSNSNELKRLEALLERERGRSEALEQQDAGLRQQIELITKREAELQRIASEYEKDLALRQHNYKVAMQKVEQEIELRKKTEALLVETQRNLENEQKTRARDLNINDKVVSLEKQLLEMEQSYKTETENTQKLKKHNAELDFTVKSQEEKVRDMVDMIDTLQKHKEELGQENAELQALVVQEKNLRSQLKEMHKEAENKMQTLINDIERTMCREQKAQEDNRALLEKISDLEKAHAGLDFELKAAQGRYQQEVKAHQETEKSRLVSREEANLQEVKALQSKLNEEKSARIKADQHSQEKERQLSMLSVDYRQIQLRLQKLEGECRQESEKVAALQSQLDQEHSKRNALLSELSLHSSEVAHLRSRENQLQKELSTQREAKRRFEEDLTQLKSTHHEALANNRELQAQLEAEQCFSRLYKTQANENREESAERLSKIEDLEEERVSLKHQVQVAVARADSEALARSIAEETVADLEKEKTIKELELKDFVMKHRNEINAKEAALATLKEAENELHKKLGQKAAEYEDLVQQHKKQQEELALMRSSKDEEITKLLDKCKNEVLLKQVAVNKLAEVMNRRDSDLPKQKNKARSTAELRKKEKEMRRLQQELSQERDKFNQLLLKHQDLQQLCAEEQQLKQKMVMEIDCKATEIENLQSKLNETASLSSADNDPEDSQHSSLLSLTQDSVFEGWLSVPNKQNRRRGHGWKRQYVIVSSRKIIFYNSDIDKHNTTDAVLILDLSKVYHVRSVTQGDVIRADAKEIPRIFQLLYAGEGASHRPDEQSQLDVSVLHGNCNEERPGTIVHKGHEFVHITYHMPTACEVCPKPLWHMFKPPAAYECKRCRNKIHKEHVDKHDPLAPCKLNHDPRSARDMLLLAATPEDQSLWVARLLKRIQKSGYKAASYNNNSTDGSKISPSQSTRSSYKPYAVNVQRSATLPANSSLK

Target ORF information:

RefSeq Version	NM_080535.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho kinase (Rok), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080535.3

ORF Insert Sequence:

ATGCCAGCTG GACGAGAAAC TGTGACCAAG CAGCGCAGCA TGGATGTGGA ACGAAGGCGC 
CGGGCGAATA CGCTCGAGCG CGAGATGCGC GATCCGACCA GCATCTGCAA TGTGGACTGC 
CTGCTGGACA CGGTGTCGGC CTTGGTCAGC GATTGTGACC ACGAGTCCCT GAGGCGGCTT 
AAGAACATCG AGCAGTATGC GGCCAAATAC AAACCATTGG CAATGCAGAT CAATCAGCTG 
CGCATGAACG TCGAGGATTT CCATTTTATC AAACTCATCG GCGCCGGCGC CTTTGGAGAG 
GTGCAGCTGG TGCGGCACAA GTCCTCCAGC CAGGTGTACG CCATGAAGCG GCTGTCCAAA 
TTCGAGATGA TGAAGCGACC GGACTCCGCG TTCTTCTGGG AGGAGCGACA CATTATGGCG 
CACGCCAACT CCGAATGGAT CGTTCAGCTG CACTTTGCAT TTCAGGATGC CAAATACCTA 
TACATGGTGA TGGACTTTAT GCCCGGCGGC GATATAGTCT CGCTGATGGG TGACTACGAC 
ATCCCGGAGA AGTGGGCCAT CTTTTACACA ATGGAGGTGG TTCTCGCGCT GGACACCATT 
CACAATATGG GCTTCGTGCA TCGGGACGTT AAGCCGGATA ATATGCTCCT CGACAGCTAT 
GGCCACCTGA AATTGGCCGA CTTTGGCACC TGTATGCGAA TGGGCGCCAA TGGCCAAGTG 
GTGTCCAGCA ATGCCGTTGG CACGCCGGAC TACATCAGCC CGGAGGTGCT GCAGTCGCAG 
GGCGTGGACA ACGAGTACGG GCGGGAATGC GACTGGTGGT CGGTGGGCAT TTTCCTCTAC 
GAGATGCTGT TCGGCGAGAC ACCATTCTAC GCAGACTCGC TGGTGGGCAC CTACGGCAAG 
ATCATGGACC ACAAGAACTC GCTTAGCTTT CCACCCGAGG TTGAGATCAG CGAACAGGCG 
AAGGCACTGA TCCGCGCCTT TCTCACGGAC CGAACGCAAC GTTTGGGTCG CTACGGCATC 
GAGGACATTA AGGCGCATCC GTTTTTCCGA AATGATACCT GGTCGTTCGA CAACATTCGC 
GAGAGTGTGC CGCCTGTGGT GCCCGAACTC TCCTCCGACG ATGATACGCG AAATTTCGAG 
GACATTGAGC GCGATGAGAA GCCGGAGGAA GTGTTTCCCG TACCCAAGGG CTTCGATGGC 
AATCACTTGC CCTTCATCGG CTTTACGTAT ACGGGTGACT ATCAGCTGCT GTCTAGCGAC 
ACCGTGGATG CGGAGTCGAA GGAGGCCAAT GTGGCAAACA GCGGGGCGGC CAGCAATAAT 
CACGGCCACG GTCATAATCA CCGCCATCGG CCGTCGAATT CCAACGAGCT GAAGCGCCTG 
GAGGCGCTGC TCGAGCGGGA GCGTGGCAGG TCGGAAGCCT TGGAGCAGCA GGATGCTGGA 
CTGCGGCAGC AGATTGAGCT GATAACAAAA CGCGAAGCGG AGCTGCAGAG GATTGCCTCA 
GAGTACGAGA AGGATCTGGC TTTGCGTCAA CACAACTACA AGGTGGCCAT GCAGAAGGTG 
GAGCAGGAGA TCGAGCTGCG CAAGAAGACG GAGGCACTGC TCGTGGAAAC GCAGCGCAAT 
CTGGAAAACG AGCAAAAGAC GCGGGCACGC GACCTCAATA TCAACGACAA GGTGGTCTCA 
CTCGAAAAGC AGCTGCTTGA GATGGAGCAG AGCTACAAAA CGGAGACGGA GAACACTCAA 
AAGCTGAAAA AGCACAATGC CGAGCTGGAC TTCACGGTCA AGTCGCAGGA GGAGAAGGTG 
CGCGACATGG TCGACATGAT CGACACGCTA CAAAAGCATA AGGAGGAGCT CGGCCAGGAG 
AATGCCGAGC TGCAGGCGCT GGTGGTGCAG GAGAAGAATC TGCGATCGCA GCTCAAGGAG 
ATGCACAAGG AGGCCGAGAA TAAGATGCAG ACGCTGATCA ACGACATTGA ACGTACTATG 
TGTCGCGAAC AAAAGGCGCA AGAGGACAAC CGGGCGTTGC TTGAGAAGAT CAGCGACTTG 
GAGAAGGCGC ATGCCGGCCT CGACTTCGAA CTGAAGGCGG CCCAGGGCCG CTATCAGCAG 
GAGGTCAAGG CACACCAAGA GACGGAGAAG TCGCGGCTCG TCTCCCGCGA GGAGGCCAAT 
CTGCAGGAGG TAAAGGCGCT GCAGTCCAAG CTCAACGAGG AGAAGTCCGC TCGCATCAAG 
GCCGATCAGC ATTCTCAGGA AAAAGAGCGT CAGCTGAGCA TGCTGTCCGT GGACTATCGC 
CAAATCCAAC TACGTCTCCA GAAGCTCGAA GGCGAGTGCC GCCAGGAGTC GGAGAAGGTG 
GCCGCTCTGC AGTCACAGCT CGACCAGGAG CACAGCAAAC GCAATGCCTT GCTATCGGAA 
CTCAGCCTGC ACAGCTCGGA GGTGGCCCAC CTGCGATCCC GCGAAAACCA GCTACAGAAG 
GAACTGTCTA CCCAGCGCGA GGCGAAGCGA CGCTTCGAGG AGGACCTCAC CCAGCTAAAG 
AGCACACACC ACGAAGCGCT GGCCAATAAC CGTGAGCTTC AGGCCCAACT CGAGGCGGAG 
CAGTGCTTCT CGCGGCTGTA CAAGACACAG GCGAACGAGA ATCGCGAAGA GAGCGCTGAG 
CGGCTGTCGA AGATCGAGGA TCTTGAGGAG GAACGCGTCT CACTCAAGCA CCAGGTACAG 
GTGGCCGTTG CCCGTGCAGA CTCAGAGGCT TTGGCCCGAT CCATTGCCGA AGAGACGGTG 
GCGGATCTCG AAAAGGAGAA GACTATCAAG GAGCTAGAGC TGAAGGACTT CGTCATGAAG 
CATCGCAACG AGATCAACGC CAAGGAGGCG GCCCTAGCCA CGCTCAAGGA GGCCGAAAAC 
GAACTGCACA AGAAGCTGGG CCAGAAGGCA GCCGAGTACG AGGATCTTGT CCAGCAGCAC 
AAGAAGCAGC AGGAGGAGCT TGCGCTGATG AGAAGCAGCA AGGACGAAGA GATCACCAAG 
CTGCTGGATA AGTGCAAGAA CGAAGTGCTT CTCAAACAGG TGGCAGTGAA CAAGTTGGCC 
GAAGTGATGA ATCGTCGCGA CTCCGACCTG CCCAAACAAA AGAACAAGGC GCGCTCGACA 
GCCGAGCTGC GCAAAAAGGA GAAGGAGATG CGTCGGCTGC AGCAGGAGCT CTCGCAGGAG 
CGCGACAAGT TCAACCAGCT GCTGCTCAAG CACCAGGACC TGCAGCAGCT GTGCGCCGAG 
GAGCAGCAGC TCAAGCAGAA GATGGTCATG GAGATCGATT GCAAGGCCAC CGAGATCGAG 
AACCTGCAGA GCAAGCTCAA CGAGACGGCG TCGCTCTCCT CGGCGGACAA CGATCCCGAG 
GATAGCCAGC ACTCCTCTCT GCTCTCCCTC ACACAGGACT CGGTCTTCGA GGGCTGGCTA 
AGTGTGCCCA ATAAGCAGAA TCGGCGACGT GGCCACGGCT GGAAGCGGCA GTACGTCATT 
GTGTCGTCGC GCAAAATCAT CTTCTACAAT TCGGACATTG ACAAGCACAA TACCACGGAT 
GCCGTGCTCA TCCTGGACCT GAGCAAGGTG TACCACGTTC GTAGCGTCAC TCAGGGCGAT 
GTCATACGCG CCGATGCCAA AGAGATTCCA CGCATATTCC AGCTACTGTA CGCCGGCGAG 
GGCGCCTCCC ATCGTCCCGA CGAGCAGAGC CAGTTGGATG TGAGCGTGCT GCACGGTAAT 
TGCAACGAGG AGCGCCCGGG CACCATTGTC CACAAAGGTC ACGAGTTCGT TCACATTACC 
TACCACATGC CCACGGCTTG CGAGGTGTGC CCGAAGCCTT TGTGGCACAT GTTCAAGCCG 
CCGGCGGCTT ACGAATGCAA GAGATGCCGC AACAAGATAC ACAAGGAGCA TGTGGACAAA 
CACGACCCGC TGGCGCCCTG TAAGCTCAAT CACGATCCGC GCAGCGCCCG TGACATGCTG 
CTGCTGGCCG CCACGCCCGA GGATCAATCG CTGTGGGTGG CGCGCCTGCT GAAGCGTATC 
CAAAAGAGTG GATATAAGGC GGCTTCGTAC AACAACAACA GCACCGATGG CAGCAAGATA 
TCGCCCAGCC AATCGACGCG CTCCTCCTAC AAGCCGTATG CGGTTAATGT CCAACGCTCC 
GCCACGCTGC CAGCCAATTC ATCGCTGAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rok ( NM_001298407.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285336.1 Drosophila melanogaster Rho kinase (Rok), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa22201
Clone ID Related Accession (Same CDS sequence)	NM_001298407.1
Accession Version	NM_001298407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho kinase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGCTG GACGAGAAAC TGTGACCAAG CAGCGCAGCA TGGATGTGGA ACGAAGGCGC 
CGGGCGAATA CGCTCGAGCG CGAGATGCGC GATCCGACCA GCATCTGCAA TGTGGACTGC 
CTGCTGGACA CGGTGTCGGC CTTGGTCAGC GATTGTGACC ACGAGTCCCT GAGGCGGCTT 
AAGAACATCG AGCAGTATGC GGCCAAATAC AAACCATTGG CAATGCAGAT CAATCAGCTG 
CGCATGAACG TCGAGGATTT CCATTTTATC AAACTCATCG GCGCCGGCGC CTTTGGAGAG 
GTGCAGCTGG TGCGGCACAA GTCCTCCAGC CAGGTGTACG CCATGAAGCG GCTGTCCAAA 
TTCGAGATGA TGAAGCGACC GGACTCCGCG TTCTTCTGGG AGGAGCGACA CATTATGGCG 
CACGCCAACT CCGAATGGAT CGTTCAGCTG CACTTTGCAT TTCAGGATGC CAAATACCTA 
TACATGGTGA TGGACTTTAT GCCCGGCGGC GATATAGTCT CGCTGATGGG TGACTACGAC 
ATCCCGGAGA AGTGGGCCAT CTTTTACACA ATGGAGGTGG TTCTCGCGCT GGACACCATT 
CACAATATGG GCTTCGTGCA TCGGGACGTT AAGCCGGATA ATATGCTCCT CGACAGCTAT 
GGCCACCTGA AATTGGCCGA CTTTGGCACC TGTATGCGAA TGGGCGCCAA TGGCCAAGTG 
GTGTCCAGCA ATGCCGTTGG CACGCCGGAC TACATCAGCC CGGAGGTGCT GCAGTCGCAG 
GGCGTGGACA ACGAGTACGG GCGGGAATGC GACTGGTGGT CGGTGGGCAT TTTCCTCTAC 
GAGATGCTGT TCGGCGAGAC ACCATTCTAC GCAGACTCGC TGGTGGGCAC CTACGGCAAG 
ATCATGGACC ACAAGAACTC GCTTAGCTTT CCACCCGAGG TTGAGATCAG CGAACAGGCG 
AAGGCACTGA TCCGCGCCTT TCTCACGGAC CGAACGCAAC GTTTGGGTCG CTACGGCATC 
GAGGACATTA AGGCGCATCC GTTTTTCCGA AATGATACCT GGTCGTTCGA CAACATTCGC 
GAGAGTGTGC CGCCTGTGGT GCCCGAACTC TCCTCCGACG ATGATACGCG AAATTTCGAG 
GACATTGAGC GCGATGAGAA GCCGGAGGAA GTGTTTCCCG TACCCAAGGG CTTCGATGGC 
AATCACTTGC CCTTCATCGG CTTTACGTAT ACGGGTGACT ATCAGCTGCT GTCTAGCGAC 
ACCGTGGATG CGGAGTCGAA GGAGGCCAAT GTGGCAAACA GCGGGGCGGC CAGCAATAAT 
CACGGCCACG GTCATAATCA CCGCCATCGG CCGTCGAATT CCAACGAGCT GAAGCGCCTG 
GAGGCGCTGC TCGAGCGGGA GCGTGGCAGG TCGGAAGCCT TGGAGCAGCA GGATGCTGGA 
CTGCGGCAGC AGATTGAGCT GATAACAAAA CGCGAAGCGG AGCTGCAGAG GATTGCCTCA 
GAGTACGAGA AGGATCTGGC TTTGCGTCAA CACAACTACA AGGTGGCCAT GCAGAAGGTG 
GAGCAGGAGA TCGAGCTGCG CAAGAAGACG GAGGCACTGC TCGTGGAAAC GCAGCGCAAT 
CTGGAAAACG AGCAAAAGAC GCGGGCACGC GACCTCAATA TCAACGACAA GGTGGTCTCA 
CTCGAAAAGC AGCTGCTTGA GATGGAGCAG AGCTACAAAA CGGAGACGGA GAACACTCAA 
AAGCTGAAAA AGCACAATGC CGAGCTGGAC TTCACGGTCA AGTCGCAGGA GGAGAAGGTG 
CGCGACATGG TCGACATGAT CGACACGCTA CAAAAGCATA AGGAGGAGCT CGGCCAGGAG 
AATGCCGAGC TGCAGGCGCT GGTGGTGCAG GAGAAGAATC TGCGATCGCA GCTCAAGGAG 
ATGCACAAGG AGGCCGAGAA TAAGATGCAG ACGCTGATCA ACGACATTGA ACGTACTATG 
TGTCGCGAAC AAAAGGCGCA AGAGGACAAC CGGGCGTTGC TTGAGAAGAT CAGCGACTTG 
GAGAAGGCGC ATGCCGGCCT CGACTTCGAA CTGAAGGCGG CCCAGGGCCG CTATCAGCAG 
GAGGTCAAGG CACACCAAGA GACGGAGAAG TCGCGGCTCG TCTCCCGCGA GGAGGCCAAT 
CTGCAGGAGG TAAAGGCGCT GCAGTCCAAG CTCAACGAGG AGAAGTCCGC TCGCATCAAG 
GCCGATCAGC ATTCTCAGGA AAAAGAGCGT CAGCTGAGCA TGCTGTCCGT GGACTATCGC 
CAAATCCAAC TACGTCTCCA GAAGCTCGAA GGCGAGTGCC GCCAGGAGTC GGAGAAGGTG 
GCCGCTCTGC AGTCACAGCT CGACCAGGAG CACAGCAAAC GCAATGCCTT GCTATCGGAA 
CTCAGCCTGC ACAGCTCGGA GGTGGCCCAC CTGCGATCCC GCGAAAACCA GCTACAGAAG 
GAACTGTCTA CCCAGCGCGA GGCGAAGCGA CGCTTCGAGG AGGACCTCAC CCAGCTAAAG 
AGCACACACC ACGAAGCGCT GGCCAATAAC CGTGAGCTTC AGGCCCAACT CGAGGCGGAG 
CAGTGCTTCT CGCGGCTGTA CAAGACACAG GCGAACGAGA ATCGCGAAGA GAGCGCTGAG 
CGGCTGTCGA AGATCGAGGA TCTTGAGGAG GAACGCGTCT CACTCAAGCA CCAGGTACAG 
GTGGCCGTTG CCCGTGCAGA CTCAGAGGCT TTGGCCCGAT CCATTGCCGA AGAGACGGTG 
GCGGATCTCG AAAAGGAGAA GACTATCAAG GAGCTAGAGC TGAAGGACTT CGTCATGAAG 
CATCGCAACG AGATCAACGC CAAGGAGGCG GCCCTAGCCA CGCTCAAGGA GGCCGAAAAC 
GAACTGCACA AGAAGCTGGG CCAGAAGGCA GCCGAGTACG AGGATCTTGT CCAGCAGCAC 
AAGAAGCAGC AGGAGGAGCT TGCGCTGATG AGAAGCAGCA AGGACGAAGA GATCACCAAG 
CTGCTGGATA AGTGCAAGAA CGAAGTGCTT CTCAAACAGG TGGCAGTGAA CAAGTTGGCC 
GAAGTGATGA ATCGTCGCGA CTCCGACCTG CCCAAACAAA AGAACAAGGC GCGCTCGACA 
GCCGAGCTGC GCAAAAAGGA GAAGGAGATG CGTCGGCTGC AGCAGGAGCT CTCGCAGGAG 
CGCGACAAGT TCAACCAGCT GCTGCTCAAG CACCAGGACC TGCAGCAGCT GTGCGCCGAG 
GAGCAGCAGC TCAAGCAGAA GATGGTCATG GAGATCGATT GCAAGGCCAC CGAGATCGAG 
AACCTGCAGA GCAAGCTCAA CGAGACGGCG TCGCTCTCCT CGGCGGACAA CGATCCCGAG 
GATAGCCAGC ACTCCTCTCT GCTCTCCCTC ACACAGGACT CGGTCTTCGA GGGCTGGCTA 
AGTGTGCCCA ATAAGCAGAA TCGGCGACGT GGCCACGGCT GGAAGCGGCA GTACGTCATT 
GTGTCGTCGC GCAAAATCAT CTTCTACAAT TCGGACATTG ACAAGCACAA TACCACGGAT 
GCCGTGCTCA TCCTGGACCT GAGCAAGGTG TACCACGTTC GTAGCGTCAC TCAGGGCGAT 
GTCATACGCG CCGATGCCAA AGAGATTCCA CGCATATTCC AGCTACTGTA CGCCGGCGAG 
GGCGCCTCCC ATCGTCCCGA CGAGCAGAGC CAGTTGGATG TGAGCGTGCT GCACGGTAAT 
TGCAACGAGG AGCGCCCGGG CACCATTGTC CACAAAGGTC ACGAGTTCGT TCACATTACC 
TACCACATGC CCACGGCTTG CGAGGTGTGC CCGAAGCCTT TGTGGCACAT GTTCAAGCCG 
CCGGCGGCTT ACGAATGCAA GAGATGCCGC AACAAGATAC ACAAGGAGCA TGTGGACAAA 
CACGACCCGC TGGCGCCCTG TAAGCTCAAT CACGATCCGC GCAGCGCCCG TGACATGCTG 
CTGCTGGCCG CCACGCCCGA GGATCAATCG CTGTGGGTGG CGCGCCTGCT GAAGCGTATC 
CAAAAGAGTG GATATAAGGC GGCTTCGTAC AACAACAACA GCACCGATGG CAGCAAGATA 
TCGCCCAGAT CGTCTGGAGC GGCATATAAG ATCCCACAAC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285336.1
CDS	1066..5190
Translation	MPAGRETVTKQRSMDVERRRRANTLEREMRDPTSICNVDCLLDTVSALVSDCDHESLRRLKNIEQYAAKYKPLAMQINQLRMNVEDFHFIKLIGAGAFGEVQLVRHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQLHFAFQDAKYLYMVMDFMPGGDIVSLMGDYDIPEKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNMLLDSYGHLKLADFGTCMRMGANGQVVSSNAVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLYEMLFGETPFYADSLVGTYGKIMDHKNSLSFPPEVEISEQAKALIRAFLTDRTQRLGRYGIEDIKAHPFFRNDTWSFDNIRESVPPVVPELSSDDDTRNFEDIERDEKPEEVFPVPKGFDGNHLPFIGFTYTGDYQLLSSDTVDAESKEANVANSGAASNNHGHGHNHRHRPSNSNELKRLEALLERERGRSEALEQQDAGLRQQIELITKREAELQRIASEYEKDLALRQHNYKVAMQKVEQEIELRKKTEALLVETQRNLENEQKTRARDLNINDKVVSLEKQLLEMEQSYKTETENTQKLKKHNAELDFTVKSQEEKVRDMVDMIDTLQKHKEELGQENAELQALVVQEKNLRSQLKEMHKEAENKMQTLINDIERTMCREQKAQEDNRALLEKISDLEKAHAGLDFELKAAQGRYQQEVKAHQETEKSRLVSREEANLQEVKALQSKLNEEKSARIKADQHSQEKERQLSMLSVDYRQIQLRLQKLEGECRQESEKVAALQSQLDQEHSKRNALLSELSLHSSEVAHLRSRENQLQKELSTQREAKRRFEEDLTQLKSTHHEALANNRELQAQLEAEQCFSRLYKTQANENREESAERLSKIEDLEEERVSLKHQVQVAVARADSEALARSIAEETVADLEKEKTIKELELKDFVMKHRNEINAKEAALATLKEAENELHKKLGQKAAEYEDLVQQHKKQQEELALMRSSKDEEITKLLDKCKNEVLLKQVAVNKLAEVMNRRDSDLPKQKNKARSTAELRKKEKEMRRLQQELSQERDKFNQLLLKHQDLQQLCAEEQQLKQKMVMEIDCKATEIENLQSKLNETASLSSADNDPEDSQHSSLLSLTQDSVFEGWLSVPNKQNRRRGHGWKRQYVIVSSRKIIFYNSDIDKHNTTDAVLILDLSKVYHVRSVTQGDVIRADAKEIPRIFQLLYAGEGASHRPDEQSQLDVSVLHGNCNEERPGTIVHKGHEFVHITYHMPTACEVCPKPLWHMFKPPAAYECKRCRNKIHKEHVDKHDPLAPCKLNHDPRSARDMLLLAATPEDQSLWVARLLKRIQKSGYKAASYNNNSTDGSKISPRSSGAAYKIPQR

Target ORF information:

RefSeq Version	NM_001298407.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho kinase (Rok), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298407.1

ORF Insert Sequence:

ATGCCAGCTG GACGAGAAAC TGTGACCAAG CAGCGCAGCA TGGATGTGGA ACGAAGGCGC 
CGGGCGAATA CGCTCGAGCG CGAGATGCGC GATCCGACCA GCATCTGCAA TGTGGACTGC 
CTGCTGGACA CGGTGTCGGC CTTGGTCAGC GATTGTGACC ACGAGTCCCT GAGGCGGCTT 
AAGAACATCG AGCAGTATGC GGCCAAATAC AAACCATTGG CAATGCAGAT CAATCAGCTG 
CGCATGAACG TCGAGGATTT CCATTTTATC AAACTCATCG GCGCCGGCGC CTTTGGAGAG 
GTGCAGCTGG TGCGGCACAA GTCCTCCAGC CAGGTGTACG CCATGAAGCG GCTGTCCAAA 
TTCGAGATGA TGAAGCGACC GGACTCCGCG TTCTTCTGGG AGGAGCGACA CATTATGGCG 
CACGCCAACT CCGAATGGAT CGTTCAGCTG CACTTTGCAT TTCAGGATGC CAAATACCTA 
TACATGGTGA TGGACTTTAT GCCCGGCGGC GATATAGTCT CGCTGATGGG TGACTACGAC 
ATCCCGGAGA AGTGGGCCAT CTTTTACACA ATGGAGGTGG TTCTCGCGCT GGACACCATT 
CACAATATGG GCTTCGTGCA TCGGGACGTT AAGCCGGATA ATATGCTCCT CGACAGCTAT 
GGCCACCTGA AATTGGCCGA CTTTGGCACC TGTATGCGAA TGGGCGCCAA TGGCCAAGTG 
GTGTCCAGCA ATGCCGTTGG CACGCCGGAC TACATCAGCC CGGAGGTGCT GCAGTCGCAG 
GGCGTGGACA ACGAGTACGG GCGGGAATGC GACTGGTGGT CGGTGGGCAT TTTCCTCTAC 
GAGATGCTGT TCGGCGAGAC ACCATTCTAC GCAGACTCGC TGGTGGGCAC CTACGGCAAG 
ATCATGGACC ACAAGAACTC GCTTAGCTTT CCACCCGAGG TTGAGATCAG CGAACAGGCG 
AAGGCACTGA TCCGCGCCTT TCTCACGGAC CGAACGCAAC GTTTGGGTCG CTACGGCATC 
GAGGACATTA AGGCGCATCC GTTTTTCCGA AATGATACCT GGTCGTTCGA CAACATTCGC 
GAGAGTGTGC CGCCTGTGGT GCCCGAACTC TCCTCCGACG ATGATACGCG AAATTTCGAG 
GACATTGAGC GCGATGAGAA GCCGGAGGAA GTGTTTCCCG TACCCAAGGG CTTCGATGGC 
AATCACTTGC CCTTCATCGG CTTTACGTAT ACGGGTGACT ATCAGCTGCT GTCTAGCGAC 
ACCGTGGATG CGGAGTCGAA GGAGGCCAAT GTGGCAAACA GCGGGGCGGC CAGCAATAAT 
CACGGCCACG GTCATAATCA CCGCCATCGG CCGTCGAATT CCAACGAGCT GAAGCGCCTG 
GAGGCGCTGC TCGAGCGGGA GCGTGGCAGG TCGGAAGCCT TGGAGCAGCA GGATGCTGGA 
CTGCGGCAGC AGATTGAGCT GATAACAAAA CGCGAAGCGG AGCTGCAGAG GATTGCCTCA 
GAGTACGAGA AGGATCTGGC TTTGCGTCAA CACAACTACA AGGTGGCCAT GCAGAAGGTG 
GAGCAGGAGA TCGAGCTGCG CAAGAAGACG GAGGCACTGC TCGTGGAAAC GCAGCGCAAT 
CTGGAAAACG AGCAAAAGAC GCGGGCACGC GACCTCAATA TCAACGACAA GGTGGTCTCA 
CTCGAAAAGC AGCTGCTTGA GATGGAGCAG AGCTACAAAA CGGAGACGGA GAACACTCAA 
AAGCTGAAAA AGCACAATGC CGAGCTGGAC TTCACGGTCA AGTCGCAGGA GGAGAAGGTG 
CGCGACATGG TCGACATGAT CGACACGCTA CAAAAGCATA AGGAGGAGCT CGGCCAGGAG 
AATGCCGAGC TGCAGGCGCT GGTGGTGCAG GAGAAGAATC TGCGATCGCA GCTCAAGGAG 
ATGCACAAGG AGGCCGAGAA TAAGATGCAG ACGCTGATCA ACGACATTGA ACGTACTATG 
TGTCGCGAAC AAAAGGCGCA AGAGGACAAC CGGGCGTTGC TTGAGAAGAT CAGCGACTTG 
GAGAAGGCGC ATGCCGGCCT CGACTTCGAA CTGAAGGCGG CCCAGGGCCG CTATCAGCAG 
GAGGTCAAGG CACACCAAGA GACGGAGAAG TCGCGGCTCG TCTCCCGCGA GGAGGCCAAT 
CTGCAGGAGG TAAAGGCGCT GCAGTCCAAG CTCAACGAGG AGAAGTCCGC TCGCATCAAG 
GCCGATCAGC ATTCTCAGGA AAAAGAGCGT CAGCTGAGCA TGCTGTCCGT GGACTATCGC 
CAAATCCAAC TACGTCTCCA GAAGCTCGAA GGCGAGTGCC GCCAGGAGTC GGAGAAGGTG 
GCCGCTCTGC AGTCACAGCT CGACCAGGAG CACAGCAAAC GCAATGCCTT GCTATCGGAA 
CTCAGCCTGC ACAGCTCGGA GGTGGCCCAC CTGCGATCCC GCGAAAACCA GCTACAGAAG 
GAACTGTCTA CCCAGCGCGA GGCGAAGCGA CGCTTCGAGG AGGACCTCAC CCAGCTAAAG 
AGCACACACC ACGAAGCGCT GGCCAATAAC CGTGAGCTTC AGGCCCAACT CGAGGCGGAG 
CAGTGCTTCT CGCGGCTGTA CAAGACACAG GCGAACGAGA ATCGCGAAGA GAGCGCTGAG 
CGGCTGTCGA AGATCGAGGA TCTTGAGGAG GAACGCGTCT CACTCAAGCA CCAGGTACAG 
GTGGCCGTTG CCCGTGCAGA CTCAGAGGCT TTGGCCCGAT CCATTGCCGA AGAGACGGTG 
GCGGATCTCG AAAAGGAGAA GACTATCAAG GAGCTAGAGC TGAAGGACTT CGTCATGAAG 
CATCGCAACG AGATCAACGC CAAGGAGGCG GCCCTAGCCA CGCTCAAGGA GGCCGAAAAC 
GAACTGCACA AGAAGCTGGG CCAGAAGGCA GCCGAGTACG AGGATCTTGT CCAGCAGCAC 
AAGAAGCAGC AGGAGGAGCT TGCGCTGATG AGAAGCAGCA AGGACGAAGA GATCACCAAG 
CTGCTGGATA AGTGCAAGAA CGAAGTGCTT CTCAAACAGG TGGCAGTGAA CAAGTTGGCC 
GAAGTGATGA ATCGTCGCGA CTCCGACCTG CCCAAACAAA AGAACAAGGC GCGCTCGACA 
GCCGAGCTGC GCAAAAAGGA GAAGGAGATG CGTCGGCTGC AGCAGGAGCT CTCGCAGGAG 
CGCGACAAGT TCAACCAGCT GCTGCTCAAG CACCAGGACC TGCAGCAGCT GTGCGCCGAG 
GAGCAGCAGC TCAAGCAGAA GATGGTCATG GAGATCGATT GCAAGGCCAC CGAGATCGAG 
AACCTGCAGA GCAAGCTCAA CGAGACGGCG TCGCTCTCCT CGGCGGACAA CGATCCCGAG 
GATAGCCAGC ACTCCTCTCT GCTCTCCCTC ACACAGGACT CGGTCTTCGA GGGCTGGCTA 
AGTGTGCCCA ATAAGCAGAA TCGGCGACGT GGCCACGGCT GGAAGCGGCA GTACGTCATT 
GTGTCGTCGC GCAAAATCAT CTTCTACAAT TCGGACATTG ACAAGCACAA TACCACGGAT 
GCCGTGCTCA TCCTGGACCT GAGCAAGGTG TACCACGTTC GTAGCGTCAC TCAGGGCGAT 
GTCATACGCG CCGATGCCAA AGAGATTCCA CGCATATTCC AGCTACTGTA CGCCGGCGAG 
GGCGCCTCCC ATCGTCCCGA CGAGCAGAGC CAGTTGGATG TGAGCGTGCT GCACGGTAAT 
TGCAACGAGG AGCGCCCGGG CACCATTGTC CACAAAGGTC ACGAGTTCGT TCACATTACC 
TACCACATGC CCACGGCTTG CGAGGTGTGC CCGAAGCCTT TGTGGCACAT GTTCAAGCCG 
CCGGCGGCTT ACGAATGCAA GAGATGCCGC AACAAGATAC ACAAGGAGCA TGTGGACAAA 
CACGACCCGC TGGCGCCCTG TAAGCTCAAT CACGATCCGC GCAGCGCCCG TGACATGCTG 
CTGCTGGCCG CCACGCCCGA GGATCAATCG CTGTGGGTGG CGCGCCTGCT GAAGCGTATC 
CAAAAGAGTG GATATAAGGC GGCTTCGTAC AACAACAACA GCACCGATGG CAGCAAGATA 
TCGCCCAGAT CGTCTGGAGC GGCATATAAG ATCCCACAAC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rok ( NM_001298406.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285335.1 Drosophila melanogaster Rho kinase (Rok), transcript variant B, mRNA.

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CloneID	OFa22202
Clone ID Related Accession (Same CDS sequence)	NM_001298406.1
Accession Version	NM_001298406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGCTG GACGAGAAAC TGTGACCAAG CAGCGCAGCA TGGATGTGGA ACGAAGGCGC 
CGGGCGAATA CGCTCGAGCG CGAGATGCGC GATCCGACCA GCATCTGCAA TGTGGACTGC 
CTGCTGGACA CGGTGTCGGC CTTGGTCAGC GATTGTGACC ACGAGTCCCT GAGGCGGCTT 
AAGAACATCG AGCAGTATGC GGCCAAATAC AAACCATTGG CAATGCAGAT CAATCAGCTG 
CGCATGAACG TCGAGGATTT CCATTTTATC AAACTCATCG GCGCCGGCGC CTTTGGAGAG 
GTGCAGCTGG TGCGGCACAA GTCCTCCAGC CAGGTGTACG CCATGAAGCG GCTGTCCAAA 
TTCGAGATGA TGAAGCGACC GGACTCCGCG TTCTTCTGGG AGGAGCGACA CATTATGGCG 
CACGCCAACT CCGAATGGAT CGTTCAGCTG CACTTTGCAT TTCAGGATGC CAAATACCTA 
TACATGGTGA TGGACTTTAT GCCCGGCGGC GATATAGTCT CGCTGATGGG TGACTACGAC 
ATCCCGGAGA AGTGGGCCAT CTTTTACACA ATGGAGGTGG TTCTCGCGCT GGACACCATT 
CACAATATGG GCTTCGTGCA TCGGGACGTT AAGCCGGATA ATATGCTCCT CGACAGCTAT 
GGCCACCTGA AATTGGCCGA CTTTGGCACC TGTATGCGAA TGGGCGCCAA TGGCCAAGTG 
GTGTCCAGCA ATGCCGTTGG CACGCCGGAC TACATCAGCC CGGAGGTGCT GCAGTCGCAG 
GGCGTGGACA ACGAGTACGG GCGGGAATGC GACTGGTGGT CGGTGGGCAT TTTCCTCTAC 
GAGATGCTGT TCGGCGAGAC ACCATTCTAC GCAGACTCGC TGGTGGGCAC CTACGGCAAG 
ATCATGGACC ACAAGAACTC GCTTAGCTTT CCACCCGAGG TTGAGATCAG CGAACAGGCG 
AAGGCACTGA TCCGCGCCTT TCTCACGGAC CGAACGCAAC GTTTGGGTCG CTACGGCATC 
GAGGACATTA AGGCGCATCC GTTTTTCCGA AATGATACCT GGTCGTTCGA CAACATTCGC 
GAGAGTGTGC CGCCTGTGGT GCCCGAACTC TCCTCCGACG ATGATACGCG AAATTTCGAG 
GACATTGAGC GCGATGAGAA GCCGGAGGAA GTGTTTCCCG TACCCAAGGG CTTCGATGGC 
AATCACTTGC CCTTCATCGG CTTTACGTAT ACGGGTGACT ATCAGCTGCT GTCTAGCGAC 
ACCGTGGATG CGGAGTCGAA GGAGGCCAAT GTGGCAAACA GCGGGGCGGC CAGCAATAAT 
CACGGCCACG GTCATAATCA CCGCCATCGG CCGTCGAATT CCAACGAGCT GAAGCGCCTG 
GAGGCGCTGC TCGAGCGGGA GCGTGGCAGG TCGGAAGCCT TGGAGCAGCA GGATGCTGGA 
CTGCGGCAGC AGATTGAGCT GATAACAAAA CGCGAAGCGG AGCTGCAGAG GATTGCCTCA 
GAGTACGAGA AGGATCTGGC TTTGCGTCAA CACAACTACA AGGTGGCCAT GCAGAAGGTG 
GAGCAGGAGA TCGAGCTGCG CAAGAAGACG GAGGCACTGC TCGTGGAAAC GCAGCGCAAT 
CTGGAAAACG AGCAAAAGAC GCGGGCACGC GACCTCAATA TCAACGACAA GGTGGTCTCA 
CTCGAAAAGC AGCTGCTTGA GATGGAGCAG AGCTACAAAA CGGAGACGGA GAACACTCAA 
AAGCTGAAAA AGCACAATGC CGAGCTGGAC TTCACGGTCA AGTCGCAGGA GGAGAAGGTG 
CGCGACATGG TCGACATGAT CGACACGCTA CAAAAGCATA AGGAGGAGCT CGGCCAGGAG 
AATGCCGAGC TGCAGGCGCT GGTGGTGCAG GAGAAGAATC TGCGATCGCA GCTCAAGGAG 
ATGCACAAGG AGGCCGAGAA TAAGATGCAG ACGCTGATCA ACGACATTGA ACGTACTATG 
TGTCGCGAAC AAAAGGCGCA AGAGGACAAC CGGGCGTTGC TTGAGAAGAT CAGCGACTTG 
GAGAAGGCGC ATGCCGGCCT CGACTTCGAA CTGAAGGCGG CCCAGGGCCG CTATCAGCAG 
GAGGTCAAGG CACACCAAGA GACGGAGAAG TCGCGGCTCG TCTCCCGCGA GGAGGCCAAT 
CTGCAGGAGG TAAAGGCGCT GCAGTCCAAG CTCAACGAGG AGAAGTCCGC TCGCATCAAG 
GCCGATCAGC ATTCTCAGGA AAAAGAGCGT CAGCTGAGCA TGCTGTCCGT GGACTATCGC 
CAAATCCAAC TACGTCTCCA GAAGCTCGAA GGCGAGTGCC GCCAGGAGTC GGAGAAGGTG 
GCCGCTCTGC AGTCACAGCT CGACCAGGAG CACAGCAAAC GCAATGCCTT GCTATCGGAA 
CTCAGCCTGC ACAGCTCGGA GGTGGCCCAC CTGCGATCCC GCGAAAACCA GCTACAGAAG 
GAACTGTCTA CCCAGCGCGA GGCGAAGCGA CGCTTCGAGG AGGACCTCAC CCAGCTAAAG 
AGCACACACC ACGAAGCGCT GGCCAATAAC CGTGAGCTTC AGGCCCAACT CGAGGCGGAG 
CAGTGCTTCT CGCGGCTGTA CAAGACACAG GCGAACGAGA ATCGCGAAGA GAGCGCTGAG 
CGGCTGTCGA AGATCGAGGA TCTTGAGGAG GAACGCGTCT CACTCAAGCA CCAGGTACAG 
GTGGCCGTTG CCCGTGCAGA CTCAGAGGCT TTGGCCCGAT CCATTGCCGA AGAGACGGTG 
GCGGATCTCG AAAAGGAGAA GACTATCAAG GAGCTAGAGC TGAAGGACTT CGTCATGAAG 
CATCGCAACG AGATCAACGC CAAGGAGGCG GCCCTAGCCA CGCTCAAGGA GGCCGAAAAC 
GAACTGCACA AGAAGCTGGG CCAGAAGGCA GCCGAGTACG AGGATCTTGT CCAGCAGCAC 
AAGAAGCAGC AGGAGGAGCT TGCGCTGATG AGAAGCAGCA AGGACGAAGA GATCACCAAG 
CTGCTGGATA AGTGCAAGAA CGAAGTGCTT CTCAAACAGG TGGCAGTGAA CAAGTTGGCC 
GAAGTGATGA ATCGTCGCGA CTCCGACCTG CCCAAACAAA AGAACAAGGC GCGCTCGACA 
GCCGAGCTGC GCAAAAAGGA GAAGGAGATG CGTCGGCTGC AGCAGGAGCT CTCGCAGGAG 
CGCGACAAGT TCAACCAGCT GCTGCTCAAG CACCAGGACC TGCAGCAGCT GTGCGCCGAG 
GAGCAGCAGC TCAAGCAGAA GATGGTCATG GAGATCGATT GCAAGGCCAC CGAGATCGAG 
AACCTGCAGA GCAAGCTCAA CGAGACGGCG TCGCTCTCCT CGGCGGACAA CGATCCCGAG 
GATAGCCAGG ACTCGGTCTT CGAGGGCTGG CTAAGTGTGC CCAATAAGCA GAATCGGCGA 
CGTGGCCACG GCTGGAAGCG GCAGTACGTC ATTGTGTCGT CGCGCAAAAT CATCTTCTAC 
AATTCGGACA TTGACAAGCA CAATACCACG GATGCCGTGC TCATCCTGGA CCTGAGCAAG 
GTGTACCACG TTCGTAGCGT CACTCAGGGC GATGTCATAC GCGCCGATGC CAAAGAGATT 
CCACGCATAT TCCAGCTACT GTACGCCGGC GAGGGCGCCT CCCATCGTCC CGACGAGCAG 
AGCCAGTTGG ATGTGAGCGT GCTGCACGGT AATTGCAACG AGGAGCGCCC GGGCACCATT 
GTCCACAAAG GTCACGAGTT CGTTCACATT ACCTACCACA TGCCCACGGC TTGCGAGGTG 
TGCCCGAAGC CTTTGTGGCA CATGTTCAAG CCGCCGGCGG CTTACGAATG CAAGAGATGC 
CGCAACAAGA TACACAAGGA GCATGTGGAC AAACACGACC CGCTGGCGCC CTGTAAGCTC 
AATCACGATC CGCGCAGCGC CCGTGACATG CTGCTGCTGG CCGCCACGCC CGAGGATCAA 
TCGCTGTGGG TGGCGCGCCT GCTGAAGCGT ATCCAAAAGA GTGGATATAA GGCGGCTTCG 
TACAACAACA ACAGCACCGA TGGCAGCAAG ATATCGCCCA GCCAATCGAC GCGCTCCTCC 
TACAAGCCGT ATGCGGTTAA TGTCCAACGC TCCGCCACGC TGCCAGCCAA TTCATCGCTG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285335.1
CDS	1066..5211
Translation	MPAGRETVTKQRSMDVERRRRANTLEREMRDPTSICNVDCLLDTVSALVSDCDHESLRRLKNIEQYAAKYKPLAMQINQLRMNVEDFHFIKLIGAGAFGEVQLVRHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQLHFAFQDAKYLYMVMDFMPGGDIVSLMGDYDIPEKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNMLLDSYGHLKLADFGTCMRMGANGQVVSSNAVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLYEMLFGETPFYADSLVGTYGKIMDHKNSLSFPPEVEISEQAKALIRAFLTDRTQRLGRYGIEDIKAHPFFRNDTWSFDNIRESVPPVVPELSSDDDTRNFEDIERDEKPEEVFPVPKGFDGNHLPFIGFTYTGDYQLLSSDTVDAESKEANVANSGAASNNHGHGHNHRHRPSNSNELKRLEALLERERGRSEALEQQDAGLRQQIELITKREAELQRIASEYEKDLALRQHNYKVAMQKVEQEIELRKKTEALLVETQRNLENEQKTRARDLNINDKVVSLEKQLLEMEQSYKTETENTQKLKKHNAELDFTVKSQEEKVRDMVDMIDTLQKHKEELGQENAELQALVVQEKNLRSQLKEMHKEAENKMQTLINDIERTMCREQKAQEDNRALLEKISDLEKAHAGLDFELKAAQGRYQQEVKAHQETEKSRLVSREEANLQEVKALQSKLNEEKSARIKADQHSQEKERQLSMLSVDYRQIQLRLQKLEGECRQESEKVAALQSQLDQEHSKRNALLSELSLHSSEVAHLRSRENQLQKELSTQREAKRRFEEDLTQLKSTHHEALANNRELQAQLEAEQCFSRLYKTQANENREESAERLSKIEDLEEERVSLKHQVQVAVARADSEALARSIAEETVADLEKEKTIKELELKDFVMKHRNEINAKEAALATLKEAENELHKKLGQKAAEYEDLVQQHKKQQEELALMRSSKDEEITKLLDKCKNEVLLKQVAVNKLAEVMNRRDSDLPKQKNKARSTAELRKKEKEMRRLQQELSQERDKFNQLLLKHQDLQQLCAEEQQLKQKMVMEIDCKATEIENLQSKLNETASLSSADNDPEDSQDSVFEGWLSVPNKQNRRRGHGWKRQYVIVSSRKIIFYNSDIDKHNTTDAVLILDLSKVYHVRSVTQGDVIRADAKEIPRIFQLLYAGEGASHRPDEQSQLDVSVLHGNCNEERPGTIVHKGHEFVHITYHMPTACEVCPKPLWHMFKPPAAYECKRCRNKIHKEHVDKHDPLAPCKLNHDPRSARDMLLLAATPEDQSLWVARLLKRIQKSGYKAASYNNNSTDGSKISPSQSTRSSYKPYAVNVQRSATLPANSSLK

Target ORF information:

RefSeq Version	NM_001298406.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho kinase (Rok), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298406.1

ORF Insert Sequence:

ATGCCAGCTG GACGAGAAAC TGTGACCAAG CAGCGCAGCA TGGATGTGGA ACGAAGGCGC 
CGGGCGAATA CGCTCGAGCG CGAGATGCGC GATCCGACCA GCATCTGCAA TGTGGACTGC 
CTGCTGGACA CGGTGTCGGC CTTGGTCAGC GATTGTGACC ACGAGTCCCT GAGGCGGCTT 
AAGAACATCG AGCAGTATGC GGCCAAATAC AAACCATTGG CAATGCAGAT CAATCAGCTG 
CGCATGAACG TCGAGGATTT CCATTTTATC AAACTCATCG GCGCCGGCGC CTTTGGAGAG 
GTGCAGCTGG TGCGGCACAA GTCCTCCAGC CAGGTGTACG CCATGAAGCG GCTGTCCAAA 
TTCGAGATGA TGAAGCGACC GGACTCCGCG TTCTTCTGGG AGGAGCGACA CATTATGGCG 
CACGCCAACT CCGAATGGAT CGTTCAGCTG CACTTTGCAT TTCAGGATGC CAAATACCTA 
TACATGGTGA TGGACTTTAT GCCCGGCGGC GATATAGTCT CGCTGATGGG TGACTACGAC 
ATCCCGGAGA AGTGGGCCAT CTTTTACACA ATGGAGGTGG TTCTCGCGCT GGACACCATT 
CACAATATGG GCTTCGTGCA TCGGGACGTT AAGCCGGATA ATATGCTCCT CGACAGCTAT 
GGCCACCTGA AATTGGCCGA CTTTGGCACC TGTATGCGAA TGGGCGCCAA TGGCCAAGTG 
GTGTCCAGCA ATGCCGTTGG CACGCCGGAC TACATCAGCC CGGAGGTGCT GCAGTCGCAG 
GGCGTGGACA ACGAGTACGG GCGGGAATGC GACTGGTGGT CGGTGGGCAT TTTCCTCTAC 
GAGATGCTGT TCGGCGAGAC ACCATTCTAC GCAGACTCGC TGGTGGGCAC CTACGGCAAG 
ATCATGGACC ACAAGAACTC GCTTAGCTTT CCACCCGAGG TTGAGATCAG CGAACAGGCG 
AAGGCACTGA TCCGCGCCTT TCTCACGGAC CGAACGCAAC GTTTGGGTCG CTACGGCATC 
GAGGACATTA AGGCGCATCC GTTTTTCCGA AATGATACCT GGTCGTTCGA CAACATTCGC 
GAGAGTGTGC CGCCTGTGGT GCCCGAACTC TCCTCCGACG ATGATACGCG AAATTTCGAG 
GACATTGAGC GCGATGAGAA GCCGGAGGAA GTGTTTCCCG TACCCAAGGG CTTCGATGGC 
AATCACTTGC CCTTCATCGG CTTTACGTAT ACGGGTGACT ATCAGCTGCT GTCTAGCGAC 
ACCGTGGATG CGGAGTCGAA GGAGGCCAAT GTGGCAAACA GCGGGGCGGC CAGCAATAAT 
CACGGCCACG GTCATAATCA CCGCCATCGG CCGTCGAATT CCAACGAGCT GAAGCGCCTG 
GAGGCGCTGC TCGAGCGGGA GCGTGGCAGG TCGGAAGCCT TGGAGCAGCA GGATGCTGGA 
CTGCGGCAGC AGATTGAGCT GATAACAAAA CGCGAAGCGG AGCTGCAGAG GATTGCCTCA 
GAGTACGAGA AGGATCTGGC TTTGCGTCAA CACAACTACA AGGTGGCCAT GCAGAAGGTG 
GAGCAGGAGA TCGAGCTGCG CAAGAAGACG GAGGCACTGC TCGTGGAAAC GCAGCGCAAT 
CTGGAAAACG AGCAAAAGAC GCGGGCACGC GACCTCAATA TCAACGACAA GGTGGTCTCA 
CTCGAAAAGC AGCTGCTTGA GATGGAGCAG AGCTACAAAA CGGAGACGGA GAACACTCAA 
AAGCTGAAAA AGCACAATGC CGAGCTGGAC TTCACGGTCA AGTCGCAGGA GGAGAAGGTG 
CGCGACATGG TCGACATGAT CGACACGCTA CAAAAGCATA AGGAGGAGCT CGGCCAGGAG 
AATGCCGAGC TGCAGGCGCT GGTGGTGCAG GAGAAGAATC TGCGATCGCA GCTCAAGGAG 
ATGCACAAGG AGGCCGAGAA TAAGATGCAG ACGCTGATCA ACGACATTGA ACGTACTATG 
TGTCGCGAAC AAAAGGCGCA AGAGGACAAC CGGGCGTTGC TTGAGAAGAT CAGCGACTTG 
GAGAAGGCGC ATGCCGGCCT CGACTTCGAA CTGAAGGCGG CCCAGGGCCG CTATCAGCAG 
GAGGTCAAGG CACACCAAGA GACGGAGAAG TCGCGGCTCG TCTCCCGCGA GGAGGCCAAT 
CTGCAGGAGG TAAAGGCGCT GCAGTCCAAG CTCAACGAGG AGAAGTCCGC TCGCATCAAG 
GCCGATCAGC ATTCTCAGGA AAAAGAGCGT CAGCTGAGCA TGCTGTCCGT GGACTATCGC 
CAAATCCAAC TACGTCTCCA GAAGCTCGAA GGCGAGTGCC GCCAGGAGTC GGAGAAGGTG 
GCCGCTCTGC AGTCACAGCT CGACCAGGAG CACAGCAAAC GCAATGCCTT GCTATCGGAA 
CTCAGCCTGC ACAGCTCGGA GGTGGCCCAC CTGCGATCCC GCGAAAACCA GCTACAGAAG 
GAACTGTCTA CCCAGCGCGA GGCGAAGCGA CGCTTCGAGG AGGACCTCAC CCAGCTAAAG 
AGCACACACC ACGAAGCGCT GGCCAATAAC CGTGAGCTTC AGGCCCAACT CGAGGCGGAG 
CAGTGCTTCT CGCGGCTGTA CAAGACACAG GCGAACGAGA ATCGCGAAGA GAGCGCTGAG 
CGGCTGTCGA AGATCGAGGA TCTTGAGGAG GAACGCGTCT CACTCAAGCA CCAGGTACAG 
GTGGCCGTTG CCCGTGCAGA CTCAGAGGCT TTGGCCCGAT CCATTGCCGA AGAGACGGTG 
GCGGATCTCG AAAAGGAGAA GACTATCAAG GAGCTAGAGC TGAAGGACTT CGTCATGAAG 
CATCGCAACG AGATCAACGC CAAGGAGGCG GCCCTAGCCA CGCTCAAGGA GGCCGAAAAC 
GAACTGCACA AGAAGCTGGG CCAGAAGGCA GCCGAGTACG AGGATCTTGT CCAGCAGCAC 
AAGAAGCAGC AGGAGGAGCT TGCGCTGATG AGAAGCAGCA AGGACGAAGA GATCACCAAG 
CTGCTGGATA AGTGCAAGAA CGAAGTGCTT CTCAAACAGG TGGCAGTGAA CAAGTTGGCC 
GAAGTGATGA ATCGTCGCGA CTCCGACCTG CCCAAACAAA AGAACAAGGC GCGCTCGACA 
GCCGAGCTGC GCAAAAAGGA GAAGGAGATG CGTCGGCTGC AGCAGGAGCT CTCGCAGGAG 
CGCGACAAGT TCAACCAGCT GCTGCTCAAG CACCAGGACC TGCAGCAGCT GTGCGCCGAG 
GAGCAGCAGC TCAAGCAGAA GATGGTCATG GAGATCGATT GCAAGGCCAC CGAGATCGAG 
AACCTGCAGA GCAAGCTCAA CGAGACGGCG TCGCTCTCCT CGGCGGACAA CGATCCCGAG 
GATAGCCAGG ACTCGGTCTT CGAGGGCTGG CTAAGTGTGC CCAATAAGCA GAATCGGCGA 
CGTGGCCACG GCTGGAAGCG GCAGTACGTC ATTGTGTCGT CGCGCAAAAT CATCTTCTAC 
AATTCGGACA TTGACAAGCA CAATACCACG GATGCCGTGC TCATCCTGGA CCTGAGCAAG 
GTGTACCACG TTCGTAGCGT CACTCAGGGC GATGTCATAC GCGCCGATGC CAAAGAGATT 
CCACGCATAT TCCAGCTACT GTACGCCGGC GAGGGCGCCT CCCATCGTCC CGACGAGCAG 
AGCCAGTTGG ATGTGAGCGT GCTGCACGGT AATTGCAACG AGGAGCGCCC GGGCACCATT 
GTCCACAAAG GTCACGAGTT CGTTCACATT ACCTACCACA TGCCCACGGC TTGCGAGGTG 
TGCCCGAAGC CTTTGTGGCA CATGTTCAAG CCGCCGGCGG CTTACGAATG CAAGAGATGC 
CGCAACAAGA TACACAAGGA GCATGTGGAC AAACACGACC CGCTGGCGCC CTGTAAGCTC 
AATCACGATC CGCGCAGCGC CCGTGACATG CTGCTGCTGG CCGCCACGCC CGAGGATCAA 
TCGCTGTGGG TGGCGCGCCT GCTGAAGCGT ATCCAAAAGA GTGGATATAA GGCGGCTTCG 
TACAACAACA ACAGCACCGA TGGCAGCAAG ATATCGCCCA GCCAATCGAC GCGCTCCTCC 
TACAAGCCGT ATGCGGTTAA TGTCCAACGC TCCGCCACGC TGCCAGCCAA TTCATCGCTG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rok ( NM_001298408.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285337.1 Drosophila melanogaster Rho kinase (Rok), transcript variant D, mRNA.

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CloneID	OFa22203
Clone ID Related Accession (Same CDS sequence)	NM_001298408.1
Accession Version	NM_001298408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho kinase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGCCG CCCTCCATTC GTGCGAGCCG GAACGGGCGA ATACGCTCGA GCGCGAGATG 
CGCGATCCGA CCAGCATCTG CAATGTGGAC TGCCTGCTGG ACACGGTGTC GGCCTTGGTC 
AGCGATTGTG ACCACGAGTC CCTGAGGCGG CTTAAGAACA TCGAGCAGTA TGCGGCCAAA 
TACAAACCAT TGGCAATGCA GATCAATCAG CTGCGCATGA ACGTCGAGGA TTTCCATTTT 
ATCAAACTCA TCGGCGCCGG CGCCTTTGGA GAGGTGCAGC TGGTGCGGCA CAAGTCCTCC 
AGCCAGGTGT ACGCCATGAA GCGGCTGTCC AAATTCGAGA TGATGAAGCG ACCGGACTCC 
GCGTTCTTCT GGGAGGAGCG ACACATTATG GCGCACGCCA ACTCCGAATG GATCGTTCAG 
CTGCACTTTG CATTTCAGGA TGCCAAATAC CTATACATGG TGATGGACTT TATGCCCGGC 
GGCGATATAG TCTCGCTGAT GGGTGACTAC GACATCCCGG AGAAGTGGGC CATCTTTTAC 
ACAATGGAGG TGGTTCTCGC GCTGGACACC ATTCACAATA TGGGCTTCGT GCATCGGGAC 
GTTAAGCCGG ATAATATGCT CCTCGACAGC TATGGCCACC TGAAATTGGC CGACTTTGGC 
ACCTGTATGC GAATGGGCGC CAATGGCCAA GTGGTGTCCA GCAATGCCGT TGGCACGCCG 
GACTACATCA GCCCGGAGGT GCTGCAGTCG CAGGGCGTGG ACAACGAGTA CGGGCGGGAA 
TGCGACTGGT GGTCGGTGGG CATTTTCCTC TACGAGATGC TGTTCGGCGA GACACCATTC 
TACGCAGACT CGCTGGTGGG CACCTACGGC AAGATCATGG ACCACAAGAA CTCGCTTAGC 
TTTCCACCCG AGGTTGAGAT CAGCGAACAG GCGAAGGCAC TGATCCGCGC CTTTCTCACG 
GACCGAACGC AACGTTTGGG TCGCTACGGC ATCGAGGACA TTAAGGCGCA TCCGTTTTTC 
CGAAATGATA CCTGGTCGTT CGACAACATT CGCGAGAGTG TGCCGCCTGT GGTGCCCGAA 
CTCTCCTCCG ACGATGATAC GCGAAATTTC GAGGACATTG AGCGCGATGA GAAGCCGGAG 
GAAGTGTTTC CCGTACCCAA GGGCTTCGAT GGCAATCACT TGCCCTTCAT CGGCTTTACG 
TATACGGGTG ACTATCAGCT GCTGTCTAGC GACACCGTGG ATGCGGAGTC GAAGGAGGCC 
AATGTGGCAA ACAGCGGGGC GGCCAGCAAT AATCACGGCC ACGGTCATAA TCACCGCCAT 
CGGCCGTCGA ATTCCAACGA GCTGAAGCGC CTGGAGGCGC TGCTCGAGCG GGAGCGTGGC 
AGGTCGGAAG CCTTGGAGCA GCAGGATGCT GGACTGCGGC AGCAGATTGA GCTGATAACA 
AAACGCGAAG CGGAGCTGCA GAGGATTGCC TCAGAGTACG AGAAGGATCT GGCTTTGCGT 
CAACACAACT ACAAGGTGGC CATGCAGAAG GTGGAGCAGG AGATCGAGCT GCGCAAGAAG 
ACGGAGGCAC TGCTCGTGGA AACGCAGCGC AATCTGGAAA ACGAGCAAAA GACGCGGGCA 
CGCGACCTCA ATATCAACGA CAAGGTGGTC TCACTCGAAA AGCAGCTGCT TGAGATGGAG 
CAGAGCTACA AAACGGAGAC GGAGAACACT CAAAAGCTGA AAAAGCACAA TGCCGAGCTG 
GACTTCACGG TCAAGTCGCA GGAGGAGAAG GTGCGCGACA TGGTCGACAT GATCGACACG 
CTACAAAAGC ATAAGGAGGA GCTCGGCCAG GAGAATGCCG AGCTGCAGGC GCTGGTGGTG 
CAGGAGAAGA ATCTGCGATC GCAGCTCAAG GAGATGCACA AGGAGGCCGA GAATAAGATG 
CAGACGCTGA TCAACGACAT TGAACGTACT ATGTGTCGCG AACAAAAGGC GCAAGAGGAC 
AACCGGGCGT TGCTTGAGAA GATCAGCGAC TTGGAGAAGG CGCATGCCGG CCTCGACTTC 
GAACTGAAGG CGGCCCAGGG CCGCTATCAG CAGGAGGTCA AGGCACACCA AGAGACGGAG 
AAGTCGCGGC TCGTCTCCCG CGAGGAGGCC AATCTGCAGG AGGTAAAGGC GCTGCAGTCC 
AAGCTCAACG AGGAGAAGTC CGCTCGCATC AAGGCCGATC AGCATTCTCA GGAAAAAGAG 
CGTCAGCTGA GCATGCTGTC CGTGGACTAT CGCCAAATCC AACTACGTCT CCAGAAGCTC 
GAAGGCGAGT GCCGCCAGGA GTCGGAGAAG GTGGCCGCTC TGCAGTCACA GCTCGACCAG 
GAGCACAGCA AACGCAATGC CTTGCTATCG GAACTCAGCC TGCACAGCTC GGAGGTGGCC 
CACCTGCGAT CCCGCGAAAA CCAGCTACAG AAGGAACTGT CTACCCAGCG CGAGGCGAAG 
CGACGCTTCG AGGAGGACCT CACCCAGCTA AAGAGCACAC ACCACGAAGC GCTGGCCAAT 
AACCGTGAGC TTCAGGCCCA ACTCGAGGCG GAGCAGTGCT TCTCGCGGCT GTACAAGACA 
CAGGCGAACG AGAATCGCGA AGAGAGCGCT GAGCGGCTGT CGAAGATCGA GGATCTTGAG 
GAGGAACGCG TCTCACTCAA GCACCAGGTA CAGGTGGCCG TTGCCCGTGC AGACTCAGAG 
GCTTTGGCCC GATCCATTGC CGAAGAGACG GTGGCGGATC TCGAAAAGGA GAAGACTATC 
AAGGAGCTAG AGCTGAAGGA CTTCGTCATG AAGCATCGCA ACGAGATCAA CGCCAAGGAG 
GCGGCCCTAG CCACGCTCAA GGAGGCCGAA AACGAACTGC ACAAGAAGCT GGGCCAGAAG 
GCAGCCGAGT ACGAGGATCT TGTCCAGCAG CACAAGAAGC AGCAGGAGGA GCTTGCGCTG 
ATGAGAAGCA GCAAGGACGA AGAGATCACC AAGCTGCTGG ATAAGTGCAA GAACGAAGTG 
CTTCTCAAAC AGGTGGCAGT GAACAAGTTG GCCGAAGTGA TGAATCGTCG CGACTCCGAC 
CTGCCCAAAC AAAAGAACAA GGCGCGCTCG ACAGCCGAGC TGCGCAAAAA GGAGAAGGAG 
ATGCGTCGGC TGCAGCAGGA GCTCTCGCAG GAGCGCGACA AGTTCAACCA GCTGCTGCTC 
AAGCACCAGG ACCTGCAGCA GCTGTGCGCC GAGGAGCAGC AGCTCAAGCA GAAGATGGTC 
ATGGAGATCG ATTGCAAGGC CACCGAGATC GAGAACCTGC AGAGCAAGCT CAACGAGACG 
GCGTCGCTCT CCTCGGCGGA CAACGATCCC GAGGATAGCC AGCACTCCTC TCTGCTCTCC 
CTCACACAGG ACTCGGTCTT CGAGGGCTGG CTAAGTGTGC CCAATAAGCA GAATCGGCGA 
CGTGGCCACG GCTGGAAGCG GCAGTACGTC ATTGTGTCGT CGCGCAAAAT CATCTTCTAC 
AATTCGGACA TTGACAAGCA CAATACCACG GATGCCGTGC TCATCCTGGA CCTGAGCAAG 
GTGTACCACG TTCGTAGCGT CACTCAGGGC GATGTCATAC GCGCCGATGC CAAAGAGATT 
CCACGCATAT TCCAGCTACT GTACGCCGGC GAGGGCGCCT CCCATCGTCC CGACGAGCAG 
AGCCAGTTGG ATGTGAGCGT GCTGCACGGT AATTGCAACG AGGAGCGCCC GGGCACCATT 
GTCCACAAAG GTCACGAGTT CGTTCACATT ACCTACCACA TGCCCACGGC TTGCGAGGTG 
TGCCCGAAGC CTTTGTGGCA CATGTTCAAG CCGCCGGCGG CTTACGAATG CAAGAGATGC 
CGCAACAAGA TACACAAGGA GCATGTGGAC AAACACGACC CGCTGGCGCC CTGTAAGCTC 
AATCACGATC CGCGCAGCGC CCGTGACATG CTGCTGCTGG CCGCCACGCC CGAGGATCAA 
TCGCTGTGGG TGGCGCGCCT GCTGAAGCGT ATCCAAAAGA GTGGATATAA GGCGGCTTCG 
TACAACAACA ACAGCACCGA TGGCAGCAAG ATATCGCCCA GCCAATCGAC GCGCTCCTCC 
TACAAGCCGT ATGCGGTTAA TGTCCAACGC TCCGCCACGC TGCCAGCCAA TTCATCGCTG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285337.1
CDS	94..4239
Translation	MPAALHSCEPERANTLEREMRDPTSICNVDCLLDTVSALVSDCDHESLRRLKNIEQYAAKYKPLAMQINQLRMNVEDFHFIKLIGAGAFGEVQLVRHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQLHFAFQDAKYLYMVMDFMPGGDIVSLMGDYDIPEKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNMLLDSYGHLKLADFGTCMRMGANGQVVSSNAVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLYEMLFGETPFYADSLVGTYGKIMDHKNSLSFPPEVEISEQAKALIRAFLTDRTQRLGRYGIEDIKAHPFFRNDTWSFDNIRESVPPVVPELSSDDDTRNFEDIERDEKPEEVFPVPKGFDGNHLPFIGFTYTGDYQLLSSDTVDAESKEANVANSGAASNNHGHGHNHRHRPSNSNELKRLEALLERERGRSEALEQQDAGLRQQIELITKREAELQRIASEYEKDLALRQHNYKVAMQKVEQEIELRKKTEALLVETQRNLENEQKTRARDLNINDKVVSLEKQLLEMEQSYKTETENTQKLKKHNAELDFTVKSQEEKVRDMVDMIDTLQKHKEELGQENAELQALVVQEKNLRSQLKEMHKEAENKMQTLINDIERTMCREQKAQEDNRALLEKISDLEKAHAGLDFELKAAQGRYQQEVKAHQETEKSRLVSREEANLQEVKALQSKLNEEKSARIKADQHSQEKERQLSMLSVDYRQIQLRLQKLEGECRQESEKVAALQSQLDQEHSKRNALLSELSLHSSEVAHLRSRENQLQKELSTQREAKRRFEEDLTQLKSTHHEALANNRELQAQLEAEQCFSRLYKTQANENREESAERLSKIEDLEEERVSLKHQVQVAVARADSEALARSIAEETVADLEKEKTIKELELKDFVMKHRNEINAKEAALATLKEAENELHKKLGQKAAEYEDLVQQHKKQQEELALMRSSKDEEITKLLDKCKNEVLLKQVAVNKLAEVMNRRDSDLPKQKNKARSTAELRKKEKEMRRLQQELSQERDKFNQLLLKHQDLQQLCAEEQQLKQKMVMEIDCKATEIENLQSKLNETASLSSADNDPEDSQHSSLLSLTQDSVFEGWLSVPNKQNRRRGHGWKRQYVIVSSRKIIFYNSDIDKHNTTDAVLILDLSKVYHVRSVTQGDVIRADAKEIPRIFQLLYAGEGASHRPDEQSQLDVSVLHGNCNEERPGTIVHKGHEFVHITYHMPTACEVCPKPLWHMFKPPAAYECKRCRNKIHKEHVDKHDPLAPCKLNHDPRSARDMLLLAATPEDQSLWVARLLKRIQKSGYKAASYNNNSTDGSKISPSQSTRSSYKPYAVNVQRSATLPANSSLK

Target ORF information:

RefSeq Version	NM_001298408.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho kinase (Rok), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298408.1

ORF Insert Sequence:

ATGCCCGCCG CCCTCCATTC GTGCGAGCCG GAACGGGCGA ATACGCTCGA GCGCGAGATG 
CGCGATCCGA CCAGCATCTG CAATGTGGAC TGCCTGCTGG ACACGGTGTC GGCCTTGGTC 
AGCGATTGTG ACCACGAGTC CCTGAGGCGG CTTAAGAACA TCGAGCAGTA TGCGGCCAAA 
TACAAACCAT TGGCAATGCA GATCAATCAG CTGCGCATGA ACGTCGAGGA TTTCCATTTT 
ATCAAACTCA TCGGCGCCGG CGCCTTTGGA GAGGTGCAGC TGGTGCGGCA CAAGTCCTCC 
AGCCAGGTGT ACGCCATGAA GCGGCTGTCC AAATTCGAGA TGATGAAGCG ACCGGACTCC 
GCGTTCTTCT GGGAGGAGCG ACACATTATG GCGCACGCCA ACTCCGAATG GATCGTTCAG 
CTGCACTTTG CATTTCAGGA TGCCAAATAC CTATACATGG TGATGGACTT TATGCCCGGC 
GGCGATATAG TCTCGCTGAT GGGTGACTAC GACATCCCGG AGAAGTGGGC CATCTTTTAC 
ACAATGGAGG TGGTTCTCGC GCTGGACACC ATTCACAATA TGGGCTTCGT GCATCGGGAC 
GTTAAGCCGG ATAATATGCT CCTCGACAGC TATGGCCACC TGAAATTGGC CGACTTTGGC 
ACCTGTATGC GAATGGGCGC CAATGGCCAA GTGGTGTCCA GCAATGCCGT TGGCACGCCG 
GACTACATCA GCCCGGAGGT GCTGCAGTCG CAGGGCGTGG ACAACGAGTA CGGGCGGGAA 
TGCGACTGGT GGTCGGTGGG CATTTTCCTC TACGAGATGC TGTTCGGCGA GACACCATTC 
TACGCAGACT CGCTGGTGGG CACCTACGGC AAGATCATGG ACCACAAGAA CTCGCTTAGC 
TTTCCACCCG AGGTTGAGAT CAGCGAACAG GCGAAGGCAC TGATCCGCGC CTTTCTCACG 
GACCGAACGC AACGTTTGGG TCGCTACGGC ATCGAGGACA TTAAGGCGCA TCCGTTTTTC 
CGAAATGATA CCTGGTCGTT CGACAACATT CGCGAGAGTG TGCCGCCTGT GGTGCCCGAA 
CTCTCCTCCG ACGATGATAC GCGAAATTTC GAGGACATTG AGCGCGATGA GAAGCCGGAG 
GAAGTGTTTC CCGTACCCAA GGGCTTCGAT GGCAATCACT TGCCCTTCAT CGGCTTTACG 
TATACGGGTG ACTATCAGCT GCTGTCTAGC GACACCGTGG ATGCGGAGTC GAAGGAGGCC 
AATGTGGCAA ACAGCGGGGC GGCCAGCAAT AATCACGGCC ACGGTCATAA TCACCGCCAT 
CGGCCGTCGA ATTCCAACGA GCTGAAGCGC CTGGAGGCGC TGCTCGAGCG GGAGCGTGGC 
AGGTCGGAAG CCTTGGAGCA GCAGGATGCT GGACTGCGGC AGCAGATTGA GCTGATAACA 
AAACGCGAAG CGGAGCTGCA GAGGATTGCC TCAGAGTACG AGAAGGATCT GGCTTTGCGT 
CAACACAACT ACAAGGTGGC CATGCAGAAG GTGGAGCAGG AGATCGAGCT GCGCAAGAAG 
ACGGAGGCAC TGCTCGTGGA AACGCAGCGC AATCTGGAAA ACGAGCAAAA GACGCGGGCA 
CGCGACCTCA ATATCAACGA CAAGGTGGTC TCACTCGAAA AGCAGCTGCT TGAGATGGAG 
CAGAGCTACA AAACGGAGAC GGAGAACACT CAAAAGCTGA AAAAGCACAA TGCCGAGCTG 
GACTTCACGG TCAAGTCGCA GGAGGAGAAG GTGCGCGACA TGGTCGACAT GATCGACACG 
CTACAAAAGC ATAAGGAGGA GCTCGGCCAG GAGAATGCCG AGCTGCAGGC GCTGGTGGTG 
CAGGAGAAGA ATCTGCGATC GCAGCTCAAG GAGATGCACA AGGAGGCCGA GAATAAGATG 
CAGACGCTGA TCAACGACAT TGAACGTACT ATGTGTCGCG AACAAAAGGC GCAAGAGGAC 
AACCGGGCGT TGCTTGAGAA GATCAGCGAC TTGGAGAAGG CGCATGCCGG CCTCGACTTC 
GAACTGAAGG CGGCCCAGGG CCGCTATCAG CAGGAGGTCA AGGCACACCA AGAGACGGAG 
AAGTCGCGGC TCGTCTCCCG CGAGGAGGCC AATCTGCAGG AGGTAAAGGC GCTGCAGTCC 
AAGCTCAACG AGGAGAAGTC CGCTCGCATC AAGGCCGATC AGCATTCTCA GGAAAAAGAG 
CGTCAGCTGA GCATGCTGTC CGTGGACTAT CGCCAAATCC AACTACGTCT CCAGAAGCTC 
GAAGGCGAGT GCCGCCAGGA GTCGGAGAAG GTGGCCGCTC TGCAGTCACA GCTCGACCAG 
GAGCACAGCA AACGCAATGC CTTGCTATCG GAACTCAGCC TGCACAGCTC GGAGGTGGCC 
CACCTGCGAT CCCGCGAAAA CCAGCTACAG AAGGAACTGT CTACCCAGCG CGAGGCGAAG 
CGACGCTTCG AGGAGGACCT CACCCAGCTA AAGAGCACAC ACCACGAAGC GCTGGCCAAT 
AACCGTGAGC TTCAGGCCCA ACTCGAGGCG GAGCAGTGCT TCTCGCGGCT GTACAAGACA 
CAGGCGAACG AGAATCGCGA AGAGAGCGCT GAGCGGCTGT CGAAGATCGA GGATCTTGAG 
GAGGAACGCG TCTCACTCAA GCACCAGGTA CAGGTGGCCG TTGCCCGTGC AGACTCAGAG 
GCTTTGGCCC GATCCATTGC CGAAGAGACG GTGGCGGATC TCGAAAAGGA GAAGACTATC 
AAGGAGCTAG AGCTGAAGGA CTTCGTCATG AAGCATCGCA ACGAGATCAA CGCCAAGGAG 
GCGGCCCTAG CCACGCTCAA GGAGGCCGAA AACGAACTGC ACAAGAAGCT GGGCCAGAAG 
GCAGCCGAGT ACGAGGATCT TGTCCAGCAG CACAAGAAGC AGCAGGAGGA GCTTGCGCTG 
ATGAGAAGCA GCAAGGACGA AGAGATCACC AAGCTGCTGG ATAAGTGCAA GAACGAAGTG 
CTTCTCAAAC AGGTGGCAGT GAACAAGTTG GCCGAAGTGA TGAATCGTCG CGACTCCGAC 
CTGCCCAAAC AAAAGAACAA GGCGCGCTCG ACAGCCGAGC TGCGCAAAAA GGAGAAGGAG 
ATGCGTCGGC TGCAGCAGGA GCTCTCGCAG GAGCGCGACA AGTTCAACCA GCTGCTGCTC 
AAGCACCAGG ACCTGCAGCA GCTGTGCGCC GAGGAGCAGC AGCTCAAGCA GAAGATGGTC 
ATGGAGATCG ATTGCAAGGC CACCGAGATC GAGAACCTGC AGAGCAAGCT CAACGAGACG 
GCGTCGCTCT CCTCGGCGGA CAACGATCCC GAGGATAGCC AGCACTCCTC TCTGCTCTCC 
CTCACACAGG ACTCGGTCTT CGAGGGCTGG CTAAGTGTGC CCAATAAGCA GAATCGGCGA 
CGTGGCCACG GCTGGAAGCG GCAGTACGTC ATTGTGTCGT CGCGCAAAAT CATCTTCTAC 
AATTCGGACA TTGACAAGCA CAATACCACG GATGCCGTGC TCATCCTGGA CCTGAGCAAG 
GTGTACCACG TTCGTAGCGT CACTCAGGGC GATGTCATAC GCGCCGATGC CAAAGAGATT 
CCACGCATAT TCCAGCTACT GTACGCCGGC GAGGGCGCCT CCCATCGTCC CGACGAGCAG 
AGCCAGTTGG ATGTGAGCGT GCTGCACGGT AATTGCAACG AGGAGCGCCC GGGCACCATT 
GTCCACAAAG GTCACGAGTT CGTTCACATT ACCTACCACA TGCCCACGGC TTGCGAGGTG 
TGCCCGAAGC CTTTGTGGCA CATGTTCAAG CCGCCGGCGG CTTACGAATG CAAGAGATGC 
CGCAACAAGA TACACAAGGA GCATGTGGAC AAACACGACC CGCTGGCGCC CTGTAAGCTC 
AATCACGATC CGCGCAGCGC CCGTGACATG CTGCTGCTGG CCGCCACGCC CGAGGATCAA 
TCGCTGTGGG TGGCGCGCCT GCTGAAGCGT ATCCAAAAGA GTGGATATAA GGCGGCTTCG 
TACAACAACA ACAGCACCGA TGGCAGCAAG ATATCGCCCA GCCAATCGAC GCGCTCCTCC 
TACAAGCCGT ATGCGGTTAA TGTCCAACGC TCCGCCACGC TGCCAGCCAA TTCATCGCTG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rok cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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