skd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	skd
Entrez Gene ID	43906
Full Name	skuld
Synonyms	CG9936,Dmel\CG9936,LD28662,MED13,Med13,Pap,Pap/Trap,SPH203,Scad78,Skd,Skd/Med13,Skd/Pap/Bli,Srb9/AMIB/PAP/TRAP240,TRAP240,Trap240,bli,bls,dMED13,dTRAP240,dTrap240,dmMED13,l(3)L7062,l(3)rK760,pap,pap/dTRAP240,siren9,skd/MED13
General protein information	Preferred Names skuld Names CG9936 gene product from transcript CG9936-RC CG9936-PC CG9936-PD CG9936-PE CG9936-PF blind spot flytrap mediator complex subunit 13 poils aux pattes skd-PC skd-PD skd-PE skd-PF suppressor of constitutively activated Dpp signaling 78
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78A2-78A2\|3-51 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275205.1	NP_001262134.1	skuld, isoform F
NM_079914.3	NP_524653.1	skuld, isoform D
NM_168879.3	NP_730590.1	skuld, isoform C
NM_168880.3	NP_730591.2	skuld, isoform E

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	med13a	XP_005172748.1
Xenopus tropicalis (tropical clawed frog)	med13	XP_004911678.1
Homo sapiens (human)	MED13	NP_005112.2
Pan troglodytes (chimpanzee)	MED13	XP_001138050.1
Drosophila melanogaster (fruit fly)	skd	NP_001262134.1
Canis lupus familiaris (dog)	MED13	XP_005625048.1
Bos taurus (cattle)	MED13	XP_002695649.2
Mus musculus (house mouse)	Med13	NP_001074400.1
	MED13	XP_001110128.2
Rattus norvegicus (Norway rat)	Med13	NP_001100505.1
Gallus gallus (chicken)	MED13	XP_004946777.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kto-Skd affects the A/P-axial development of the whole wing disc. Kto can interact with Cubitus interruptus (Ci), bind to the Ci-binding region on ptc promoter, which are both regulated by Hh signals to down-regulate ptc expression.
Title: The Kto-Skd complex can regulate ptc expression by interacting with Cubitus interruptus (Ci) in the Hedgehog signaling pathway.

The secreted peptide Wingless acts as a downstream effector of MED13 to mediate cross-talk with adipose tissue and suppress obesity.
Title: Heart- and muscle-derived signaling system dependent on MED13 and Wingless controls obesity in Drosophila.

Pygopus promotes Wnt target gene transcription by recruiting the mediator complex through interactions with Med12 and Med13.
Title: Pygopus activates Wingless target gene transcription through the mediator complex subunits Med12 and Med13.

Kto/Skd act as essential coactivators for Ato expression during early retinal neurogenesis.
Title: Drosophila TRAP230/240 are essential coactivators for Atonal in retinal neurogenesis.

Data show that both sklud and kohtalo are required to establish normal cell affinity differences at the anterior-posterior and dorsal-ventral compartment boundaries of the wing disc.
Title: Two subunits of the Drosophila mediator complex act together to control cell affinity.

The following skd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the skd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19333	NM_079914.3 Latest version!	Drosophila melanogaster skuld (skd), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa19333	NM_168879.3 Latest version!	Drosophila melanogaster skuld (skd), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa21255	NM_001275205.1 Latest version!	Drosophila melanogaster skuld (skd), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa19334	NM_168880.3 Latest version!	Drosophila melanogaster skuld (skd), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

skd ( NM_079914.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524653.1 Drosophila melanogaster skuld (skd), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa19333
Clone ID Related Accession (Same CDS sequence)	NM_079914.3 , NM_168879.3
Accession Version	NM_079914.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	skuld, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079914.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCATC AAAATCATCA GACAAACGGC GCAAGTTTGG AGGATTGCCA CACAAACTTT 
TATGCCTTGA CTGATTTATG TGGAATAAAG TGGCGGAAGT TCGTTAACGG GGAGCGACCG 
AACGCATCGA GCGATCCTTT GGCGGACCCG ATCCTGCGCT CATATTCGCG ATGCATCCAG 
GCGGACATGC TGTGCGTGTG GAGGAGGGTC CAATCCACGA AGACGGACCA CGCCGACCCG 
AATGCCTTAA CCTTCGAGAT GACCACGTCG ACCAAGGTCC ACCCGCCCCT TTCGCTGGCC 
GCCGCCAAGG AGCTATGGAT CTTCTGGTAC GGCGAGGAGC CCGATCTCAG TGAACTGGTC 
GATGCCGAGC TGCTCCGAGT AGCGGCCAAC CAAGCGCTTT GGAATGGCAC CTGGAAAGGA 
GCTCTCACCT ACGAGTGCCG GTCGCTGCTC TTTAAGGCGC TGCACAATCT CATGGAGAGA 
TTCGTGCTTA CCAAGGACAT TGTGCGCTTT GGAAAATGGT TTGTGCAGCC CTGCACCTCC 
AGCGATCGCC TCTTTGGACG CAGCTCCCAG CACTTGTCCT TCTCATTTAC GTTCTTTGTG 
CACGGCGACA CCGTTTGTGC CTCCATAGAT TTGCGCGAGC ATCCCGCCGT GCGTCCGCTG 
ACCAAGGAGC ACTTGACCGA AGCGGCGGCA GCCTTTGCAG CGGCCAGTTC TCCGCCAGGA 
TCAAATGGAT CTGCGGCGTC AGCAGGGGGA GCTGTGCCTA ATCCTGGTCA GGATCCAAAT 
GGAGCCAGCA TGGATGGACT GGACGGCGGA GAGGGAGCGG CCAAGGCAGC GCCACCTCCG 
CACGCGCGAA AGGTTATGCT GGCGCCCTTT GGAATTGCTG GCATACTTAC CGGCAATAGC 
TATAAGGCCA GCGATCCCAT AGCCGAGAAG ATCCTTGAGG ATTGGGCCTC GTTTTTTCCG 
CTGTGCAATA AGGATAACAC GGATGTGCCA CCCGTGGTGG AAGTGGTGTC GGGTGGTCAT 
AAGATGTATC ATCCCACGAA CTACGTACTA GTCACAGACC TGGACGACAT GGAGCACATG 
GAATTCGTCG AGATGCAGAA GATGCAAAGC TCAGTGGCCG GAGCGGCGGC CGAGTCAGCT 
GTTCTGGCCT CGCTTTCCTG CCCACCGGGA GCAGCATCTG CTCCTTCATC GATGGGAGCA 
GCTCCATCTG CAACAGCGGT TCCCTTGGGT CCAGCTTCCC TCAATGCGCC AGCTTTAGCA 
GGAGCAGGTG TCGGAGCCAC CGCATCTTCC GCCGCCAAAG AGATATCCCG CAAGGCAGCT 
CCACAAGCGG TTAGCGCCCT GGAACGTCTC GCCTTCCAGC CCTACTACGA TCAACGGCCC 
ACCTCGGGCT TCACCTTCAA TACCAACAAT ACTCACATAC CCGCCTCGGC AGCCGTGGAA 
ATGCCGGAAC GAACCTGGCA GGATTGCGTG ATGAACACGC TCCATGTGGA TGCAGCGGCG 
GCGGCAGCAG CAGTTGCTTC GTCTACGCCC GCCTCGGGAA CTGGGTCATT ATCCGCGGAT 
GGGGACGAGA ATGAGCAGAA TAAACCGCCA CAGGACTCCA AGCAACTGGT GCAGCAGCAG 
ATCCAGCAGC AACAGCAACG CCAAAAGCTC TGGAACTTTG TGGATCCCAT GCAAAAGGCA 
CCCTGCATAT GCACCAAACA TCTGGGAAAC ACACCTCATG GAACACCACA CGGCGGCGCT 
TCAACGTACT CCCGCAACTC CTTGGGCGGC GATTCCTCGA TGCCGGTGGC CTCCGTGGAA 
TCACCGGCAA CGCCGGCTCC CTCTCCGCAT CCGAATTCGG CGCACTCGCA ACCGACATCC 
GTGCCGCCCG CTGAGCAACT GCTCAATATG AGTCCTCATG CGCCAACTTC CGTTTCCAAT 
CTCCAACAGC CGCCGACGCC TATCGACCAC CTGCTGGACA AGAATACACC GGCGCCAACG 
CCAACGGACC AGCACGACAG CAAGAGCATT ACGGCCTCGC CCTATGTTCA TCAGACGCCA 
AGCGTGGAGC CGCCCTCGTA TACGGATCAT GCGGCCGGCG GAGGGCCAGC TGGTGGGCAG 
GGCCTTGGTA CGGGTCCAGG TAGTGTGCCC GCCCAGCAAC CGGCTACGCC CACAGCAGCA 
ACGTCAGCTG GTGGCGCCGG ATCTGGCGGA CCTTCAAATG CAATTGGAGC AAATGCGGTT 
GGTACTATAA GTGTCAAGAA GCTTGAGATG CAGCAGCAGA CTCCATCGGC AGCTATGGCC 
ATCAAGCAGG AGCCTGGTGC TCAAGGTCGG GGGGTTGGAG GTGTTACCTC CACCACGGAG 
GCCTTGAACA ACTTTAAGCG ACTGTACAAC CCGCCAAAGC TGACGTTAAA GGATCCGGAC 
AGCTTCTACG ACGAGGAGTG GCTCAAGGAG GTCATCTATG ACTTTCAGTA CCAGGAATAC 
TGGGACTATA GCACGGTGAA ACGTCCGAAG ATGGAAAAGC AACGGAGACC TAGGTATGCC 
AAGAACCTTT ACGAAGGACA AAACCACGTG AAGCCCGTGA TGCCTTCGCC AGGATCCGTT 
TATGGCTCAC AGTTGCTTTC CTTGGATGAG TCAGCGAGCC AAGCAGGAGG GAGAGGCGGT 
GGAGGACAGG CGGCGGGCAG CTCCGGCGGT GGTCTAGTGG GCATTGCCAA CAGTACTGCC 
AGTGGCAGTG ATGTAGAAGC TGATGGTAGC AGCTTCTTCC AGGGACTGAA CATTAAGACA 
GAGCCTGGAT TGCATTCCCC TTCTTGCAAG GAAACGTCGA AATCCTCGGG CGGCAACAGC 
AGCGGTGGAG GAAGTGGTAG CGGTGGTAAT CTCTTCACAG CTGAAGGCTT GAATCCCTCG 
CTCAACGATC TGGAGCAGTT GTTTGAGACA AGCTCGAACG ACGAGTGCAG CAGTGTGCAA 
ATCCACACGC CACCCGATTC TAACAACCCC TCGAATGGCG GCTGCAGTGC TGTGACCAAT 
ACGATCGAAG ACCTCAAACG GAGCACAGCG GTGGCCAGTG CTGCCGTAGC AGCGGCAGCA 
GCAGCTGCGG CCTCGGGCGC GGGCAATATC CAGGCGGAGG ATCTTACCAA GATGTTTCCC 
ACACCACCGT CTCACGAGCA GCAGCACCCG AACTCGAGTC CCTGTCAAAC GGACGTGGTT 
ATGACGGATC TCAGTGTGGA CACCACCACA ACCATTATAA CCAGTAGTAT CACCACTACT 
TGCAATACCA CTATCACGAG TAGTATCAAC ACCACCACAA CAGCCTGTAG CAACCCAAGC 
AACAGTATTA TGCTGGCTGC CGCACAAGCT CCCGTCACCG TGGTGGCCAT CCAGACTGTC 
TCCAAAATGG TAAAGCAGGA GTATAACCTG GAGCTGGGTA GTCCTATGGA AGAGCCCATT 
AATGACTGGG ATTATGTGTA CCGACCTCCG CAACAGGAGA AGTTTGTGGG CTCGACGCGA 
TATGCTCCAT TGACCAACCT GCCCAGTCAG ACACAGCCGC CGCTGACTCT GCCTACGGGA 
TGCTTTTATC AACCCACCTG GAGTAGTCAC AAATCAAGAG CAGCAACTTT GGCTAAAGCA 
GCGGCAGCTC AACAGCAGCA GCATCAGAAA CATCAGGCCC TTCAGCAGCG CATCCAATTG 
CATCAGCAGA AACTACAGCA GCTCCAGCTT CAGAATCAGC AGCAACAACA AGCGGCAGCC 
GCAGCAGCAG CGGCGGCTAG TGGAGGAGTT GGCCACCAGA AGCACCAGCA TCAGCACCTT 
CATGATCTCC TGTCAGCGGC GCCGAGGACA CCATTAACGC CCTCTACTGT TCCGCAACCG 
TTGAGCAGTG GTGGCAGTCA GTATTTGCTG AATCAGTTGA ACTGCCCACA AGCGCCACCT 
GGTGCCTCCA TGCAGCAGCT AATGCACCGA GCGGGGATGT CACCAATTTC ACCGGGACCC 
GGAATGGGTC CGTATGCCGC CAGAAGTAGT CCAATGTCGA GGGCAACCCC CACGCATCCG 
CCACCGCCAT ATCCGTATGA CTTGGCTGTG GCCAGTCCAG CTACCTCCAC ATCATCGTAC 
TTGAACCGGC CGCTTCACTC GCAAGAGCAT CCGCACATGC ATGGCTTGGG CGGCGGAGCA 
ACGGGAGTTG TGGGTCACGG AACAGGAGGT GGCGGGCACA TGGGTATGGT AGCGTACACT 
GGCGATGCGG GCATTGTCAG TGGTGGAACA GCGATGGCAG CCGGATCCTC GTCCTTGCTT 
CAGGAATTAC CAGAAGTAAA TTCTGTCCTA GTTAACATCC TGCTGTACGA TACCGCCTTG 
AATGTCTTCC GGGATCACAA CTTTGATAGT AGCAGTGTGT GTGTGTGCAA TGCGGATACG 
CAAAAGATTG GCAATATTCG CGGAGCGGAT TCCGGAGTAT ATGTACCCCT GCCCGGGGTT 
AGCTTCAATC CTTTCCCTTC CGGAGCTGGA GGCGCTTTGG CTGGACAGCG AATGCTCAAT 
GGACCCAGTT CCGCCGGTTT TGGCGGCATG CGGATGATCA GTGCCTTTGG GGGTTCGCCA 
GCCTCAGCCT CGATGCCCGG AGCTGGCTCC GGACATGGCC ATGGTCCAAA TGGTGGCTCC 
AACTCGTCGT CTTGCACGCC GCCCAGCAGT AATCCCCATA TCACCGGGTA TGTGGACGAT 
GATCCCGTGG AGTGTACGTG TGGTTTCAGT GCAGTGGTTA ATCGAAGACT CTCTCATCGC 
GCTGGACTCT TTTACGAAGA TGAGGTGGAG ATCACGGGCA TCGCGGATGA TCCGGGCAGG 
AATAAGCAGC CCACGTTGCT CAGTATCATC CAGAGTCTTA GCAGGAAAAA CCAAAACAAG 
CAGGGACCTG GAGAAACAAG CTCTGCATTG GATAAAATTG GAGCGGGAGG ATTGCCTAAT 
GGACAACTGG AGCAATTGGG TCATGCTGTA TTTGACCTGC TATTGGATCA GTGCTCTATC 
ATCCAAACAT CTAGCAGTTC GGTGCACAGA GCACTTCAGT CTCATCGGAG GCGAATGTCC 
CGCCAACGAA GGATCTTCGG TAACAATGGA GCTCCCACTG CCTCATTGGC ATCAATTGCA 
AATGTTCTAG AGTTTATGGA TGCACACGAC GTTATAAGTT TGGCCCTGGA GCAGTCGAGA 
TTGGCATTCG AAAACCAGCG GATGGACAAC ATGATGGACT TCCATGGGAA TGGTAGCAGT 
AGCTCGCATC AGCAGCAGCA ACTGACAGCT TTCCATGCGC CACCACCTGC TTTGCGTCAC 
AAGCTAGCAG GTATTGGTGC TGGGCGGTTG ACGGTTCACA AGTGGCCATA TCTTCCCGTC 
GGTTTCACCC GTAGCAATAA GGAGATTGTA CGCACCATGA ATGCTATACA GCCGATGCTG 
CAGAATGCAT TCCATTGCAA ATCCAGGGGC GGCTCAGGTT CCAAGGATGC CAGTTCGTAC 
AATACGGTGA GTGGACCGCT AACCTGGCGC CAATTCCATC GACTGGCAGG CCGTGCATCC 
GGACAATGTG AGCCGCAACC GATACCATCC GTGGTGGTGG GCTACGAGAA GGATTGGATC 
TCGGTGGCAC CGCACTCCAT CCACTATTGG GATAAGTTTC TCCTGGAACC GTACTCCTAT 
GCCAGGGATG TTGTATACGT GGTAGTGTGT CCGGACAACG AGCATGTGGT AAATTGTACT 
CGCAGCTATT TCCGGGAACT AAGCAGCACC TACGAGATGT GCAAGCTGGG CAAACACACA 
CCCATCCGAG GATGGGATGG TTTCCTTCAA GTAGGCGCTG CTCGCAACAA CGTGCCTGCG 
GATCGGGAAA CAACTCCCTT GGATGATTGG CTGCGTACCC TGGAACATGC TGCTCTGGCG 
GAGCAAATCC GTCGGTATGC AGTTGCCTTT ATTCACCAGT TGGCTCCATA CCTAAGTCGA 
GTGCCAAATG ATAAAACGCT GTTGAATCCA CCAGATGGCA GTGGCAACTC ACACTCTAAA 
GGAGGAAGCT CTTGCTCCTC GAATAGCTCT TCAGTCAGCG GATTGCCAGG CGGGGACTTA 
CCCACGGATA ATATAAAGCT GGAGCCAGGT ACAGAACCAC AAGTCCAACC GATGGAGACT 
AACGAAATAA AACAGGAACC CGGAGTGGGA AAAGGAGGCA CTGCAGCAGG TGAAACCAAA 
CCAACTCTGA TCCTCGGAGA TCCGTTGGGA ATGGGCGAGA CTTTGGAGGA CATCAACCCG 
TCAGCCATTG TCCTTTATGT GGTCAATCCG TTCACTTTCG CCTCGGATAG CTGCGAGCTG 
GAGCGTCTAG CTCTGATTGC TCTACTTCGT TGCTATGCGG AACTGCTGAA AGCAGTCCCA 
GATTCAGTGC GATCCCAGAT GAACATACAG ATTATATCGC TAGAATCGGT AATGGAACTG 
GGTCCGTGCG GCAATCGAAA GCGTTTCTCG GACGAGATTA GGTGCCTGGC TCTAAACATA 
TTTTCGCAGT GCCGGCGACA TCTGGTGCAT GCCCAATCCG TTAAAAGTCT TACTGGCTTT 
GGTACGGCGG CTAATATGGA GGCGTTTCTC AAGACAAAGG ATGAACCCAA TCGCCGGGCC 
TACAAGATGT ACACGGCTCC CTTTGTTCTG GCACCAATGC ACGAGAGGAA TGACAAAACG 
GACTTCTCCA GATCAGCGGG AAGTATGCAT GGCCAGAATG AACATCGTTA TTCGGTTATG 
TATTGCAATT ACTGCCTAAG TGAGGATCAG GCTTGGCTTT TGGCCACCGC CACTGATGAA 
AGGGGCGAGA TGCTGGAGAA GATCTGCATT AATATTGATG TGCCAAATCG TGCTCGGAGG 
AGAAAAGCCC CTGCTCGCTA TGTGGCCCTA AAGAAACTGA TGGACTTCAT CATGGGAATC 
ATCTCGCAGA CATCGCAAAT GTGGCGTTTG GTTATCGGAC GCATTGGAAG GATCGGACAC 
AGTGAACTGA AGTCGTGGAG TTTTTTGCTC AGCAAGCAAC AGCTGCAAAA GGCATCCAAG 
CAATTTAAGG ATATGTGTAA GCAATGTACA TTGATGTATC CACCCACGAT CCTGAGTGCT 
TGCCTTGTGA CCTTGGAGCC GGATGCCAAG CTGCGCGTGA TGCCCGACCA GTTTACGCCA 
GATGAGCGCT TCTCACAGAT TTCCATGCAG AACCCGTTGG CCACTCCACA GGACGTGACC 
TGCACCCACA TCCTGGTCTT TCCCACTAGC GCCGTCTGTG CGCCTTTTAC GCGCCAGTTC 
CAAAACGAGC CGCAAGTAGA TGACGACTTT CTAACATTCG AGGAAGAGGG CAACGAGGAC 
TTCAGCGATG CGGACATTGG AGATCTCTTC TGGGACACTC ATATGGACAG AGTATCCAAT 
CATGGTAGTC CCGGTCGCAT GGATGACAAT CGGAGTTGGC AGAGCGCTGG CGGTAATAAT 
TTCAAGTGCA CGCCGCCCCA GGAAGTAGAG GAGGTTGGTT CACTCAACCA GCAGCCCATA 
TCAGTGGGTT ACATGGTGTC CACGGCGCCA ACCGGTCGCA TGCCTGCTTG GTTCTGGTCC 
GCCTGTCCGC ATCTAGAAGA CGTTTGTCCG GTGTTTCTGA AGACAGCCCT CCATCTCCAT 
GTGCCCAGCA TACAGTCTGC CGACGATATT CTCAACTCGA CCAATGCCCA TCAGTCAGGC 
AACGATCATC CGCTTGACTC CAATCTCACA GCGGATGTGC TGCGCTTCGT CCTCGAGGGA 
TACAATGCCC TTTCCTGGCT GGCGCTCGAC TCCAACACAC ACGATCGTCT CTCCTGTCTG 
CCCATCAATG TCCAGACGCT AATGGATCTG TACTATCTGA CGGCTGCAAT AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524653.1
CDS	1036..8892
Translation	MTHQNHQTNGASLEDCHTNFYALTDLCGIKWRKFVNGERPNASSDPLADPILRSYSRCIQADMLCVWRRVQSTKTDHADPNALTFEMTTSTKVHPPLSLAAAKELWIFWYGEEPDLSELVDAELLRVAANQALWNGTWKGALTYECRSLLFKALHNLMERFVLTKDIVRFGKWFVQPCTSSDRLFGRSSQHLSFSFTFFVHGDTVCASIDLREHPAVRPLTKEHLTEAAAAFAAASSPPGSNGSAASAGGAVPNPGQDPNGASMDGLDGGEGAAKAAPPPHARKVMLAPFGIAGILTGNSYKASDPIAEKILEDWASFFPLCNKDNTDVPPVVEVVSGGHKMYHPTNYVLVTDLDDMEHMEFVEMQKMQSSVAGAAAESAVLASLSCPPGAASAPSSMGAAPSATAVPLGPASLNAPALAGAGVGATASSAAKEISRKAAPQAVSALERLAFQPYYDQRPTSGFTFNTNNTHIPASAAVEMPERTWQDCVMNTLHVDAAAAAAAVASSTPASGTGSLSADGDENEQNKPPQDSKQLVQQQIQQQQQRQKLWNFVDPMQKAPCICTKHLGNTPHGTPHGGASTYSRNSLGGDSSMPVASVESPATPAPSPHPNSAHSQPTSVPPAEQLLNMSPHAPTSVSNLQQPPTPIDHLLDKNTPAPTPTDQHDSKSITASPYVHQTPSVEPPSYTDHAAGGGPAGGQGLGTGPGSVPAQQPATPTAATSAGGAGSGGPSNAIGANAVGTISVKKLEMQQQTPSAAMAIKQEPGAQGRGVGGVTSTTEALNNFKRLYNPPKLTLKDPDSFYDEEWLKEVIYDFQYQEYWDYSTVKRPKMEKQRRPRYAKNLYEGQNHVKPVMPSPGSVYGSQLLSLDESASQAGGRGGGGQAAGSSGGGLVGIANSTASGSDVEADGSSFFQGLNIKTEPGLHSPSCKETSKSSGGNSSGGGSGSGGNLFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSAVTNTIEDLKRSTAVASAAVAAAAAAAASGAGNIQAEDLTKMFPTPPSHEQQHPNSSPCQTDVVMTDLSVDTTTTIITSSITTTCNTTITSSINTTTTACSNPSNSIMLAAAQAPVTVVAIQTVSKMVKQEYNLELGSPMEEPINDWDYVYRPPQQEKFVGSTRYAPLTNLPSQTQPPLTLPTGCFYQPTWSSHKSRAATLAKAAAAQQQQHQKHQALQQRIQLHQQKLQQLQLQNQQQQQAAAAAAAAASGGVGHQKHQHQHLHDLLSAAPRTPLTPSTVPQPLSSGGSQYLLNQLNCPQAPPGASMQQLMHRAGMSPISPGPGMGPYAARSSPMSRATPTHPPPPYPYDLAVASPATSTSSYLNRPLHSQEHPHMHGLGGGATGVVGHGTGGGGHMGMVAYTGDAGIVSGGTAMAAGSSSLLQELPEVNSVLVNILLYDTALNVFRDHNFDSSSVCVCNADTQKIGNIRGADSGVYVPLPGVSFNPFPSGAGGALAGQRMLNGPSSAGFGGMRMISAFGGSPASASMPGAGSGHGHGPNGGSNSSSCTPPSSNPHITGYVDDDPVECTCGFSAVVNRRLSHRAGLFYEDEVEITGIADDPGRNKQPTLLSIIQSLSRKNQNKQGPGETSSALDKIGAGGLPNGQLEQLGHAVFDLLLDQCSIIQTSSSSVHRALQSHRRRMSRQRRIFGNNGAPTASLASIANVLEFMDAHDVISLALEQSRLAFENQRMDNMMDFHGNGSSSSHQQQQLTAFHAPPPALRHKLAGIGAGRLTVHKWPYLPVGFTRSNKEIVRTMNAIQPMLQNAFHCKSRGGSGSKDASSYNTVSGPLTWRQFHRLAGRASGQCEPQPIPSVVVGYEKDWISVAPHSIHYWDKFLLEPYSYARDVVYVVVCPDNEHVVNCTRSYFRELSSTYEMCKLGKHTPIRGWDGFLQVGAARNNVPADRETTPLDDWLRTLEHAALAEQIRRYAVAFIHQLAPYLSRVPNDKTLLNPPDGSGNSHSKGGSSCSSNSSSVSGLPGGDLPTDNIKLEPGTEPQVQPMETNEIKQEPGVGKGGTAAGETKPTLILGDPLGMGETLEDINPSAIVLYVVNPFTFASDSCELERLALIALLRCYAELLKAVPDSVRSQMNIQIISLESVMELGPCGNRKRFSDEIRCLALNIFSQCRRHLVHAQSVKSLTGFGTAANMEAFLKTKDEPNRRAYKMYTAPFVLAPMHERNDKTDFSRSAGSMHGQNEHRYSVMYCNYCLSEDQAWLLATATDERGEMLEKICINIDVPNRARRRKAPARYVALKKLMDFIMGIISQTSQMWRLVIGRIGRIGHSELKSWSFLLSKQQLQKASKQFKDMCKQCTLMYPPTILSACLVTLEPDAKLRVMPDQFTPDERFSQISMQNPLATPQDVTCTHILVFPTSAVCAPFTRQFQNEPQVDDDFLTFEEEGNEDFSDADIGDLFWDTHMDRVSNHGSPGRMDDNRSWQSAGGNNFKCTPPQEVEEVGSLNQQPISVGYMVSTAPTGRMPAWFWSACPHLEDVCPVFLKTALHLHVPSIQSADDILNSTNAHQSGNDHPLDSNLTADVLRFVLEGYNALSWLALDSNTHDRLSCLPINVQTLMDLYYLTAAIA

Target ORF information:

RefSeq Version	NM_079914.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster skuld (skd), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079914.3

ORF Insert Sequence:

ATGACGCATC AAAATCATCA GACAAACGGC GCAAGTTTGG AGGATTGCCA CACAAACTTT 
TATGCCTTGA CTGATTTATG TGGAATAAAG TGGCGGAAGT TCGTTAACGG GGAGCGACCG 
AACGCATCGA GCGATCCTTT GGCGGACCCG ATCCTGCGCT CATATTCGCG ATGCATCCAG 
GCGGACATGC TGTGCGTGTG GAGGAGGGTC CAATCCACGA AGACGGACCA CGCCGACCCG 
AATGCCTTAA CCTTCGAGAT GACCACGTCG ACCAAGGTCC ACCCGCCCCT TTCGCTGGCC 
GCCGCCAAGG AGCTATGGAT CTTCTGGTAC GGCGAGGAGC CCGATCTCAG TGAACTGGTC 
GATGCCGAGC TGCTCCGAGT AGCGGCCAAC CAAGCGCTTT GGAATGGCAC CTGGAAAGGA 
GCTCTCACCT ACGAGTGCCG GTCGCTGCTC TTTAAGGCGC TGCACAATCT CATGGAGAGA 
TTCGTGCTTA CCAAGGACAT TGTGCGCTTT GGAAAATGGT TTGTGCAGCC CTGCACCTCC 
AGCGATCGCC TCTTTGGACG CAGCTCCCAG CACTTGTCCT TCTCATTTAC GTTCTTTGTG 
CACGGCGACA CCGTTTGTGC CTCCATAGAT TTGCGCGAGC ATCCCGCCGT GCGTCCGCTG 
ACCAAGGAGC ACTTGACCGA AGCGGCGGCA GCCTTTGCAG CGGCCAGTTC TCCGCCAGGA 
TCAAATGGAT CTGCGGCGTC AGCAGGGGGA GCTGTGCCTA ATCCTGGTCA GGATCCAAAT 
GGAGCCAGCA TGGATGGACT GGACGGCGGA GAGGGAGCGG CCAAGGCAGC GCCACCTCCG 
CACGCGCGAA AGGTTATGCT GGCGCCCTTT GGAATTGCTG GCATACTTAC CGGCAATAGC 
TATAAGGCCA GCGATCCCAT AGCCGAGAAG ATCCTTGAGG ATTGGGCCTC GTTTTTTCCG 
CTGTGCAATA AGGATAACAC GGATGTGCCA CCCGTGGTGG AAGTGGTGTC GGGTGGTCAT 
AAGATGTATC ATCCCACGAA CTACGTACTA GTCACAGACC TGGACGACAT GGAGCACATG 
GAATTCGTCG AGATGCAGAA GATGCAAAGC TCAGTGGCCG GAGCGGCGGC CGAGTCAGCT 
GTTCTGGCCT CGCTTTCCTG CCCACCGGGA GCAGCATCTG CTCCTTCATC GATGGGAGCA 
GCTCCATCTG CAACAGCGGT TCCCTTGGGT CCAGCTTCCC TCAATGCGCC AGCTTTAGCA 
GGAGCAGGTG TCGGAGCCAC CGCATCTTCC GCCGCCAAAG AGATATCCCG CAAGGCAGCT 
CCACAAGCGG TTAGCGCCCT GGAACGTCTC GCCTTCCAGC CCTACTACGA TCAACGGCCC 
ACCTCGGGCT TCACCTTCAA TACCAACAAT ACTCACATAC CCGCCTCGGC AGCCGTGGAA 
ATGCCGGAAC GAACCTGGCA GGATTGCGTG ATGAACACGC TCCATGTGGA TGCAGCGGCG 
GCGGCAGCAG CAGTTGCTTC GTCTACGCCC GCCTCGGGAA CTGGGTCATT ATCCGCGGAT 
GGGGACGAGA ATGAGCAGAA TAAACCGCCA CAGGACTCCA AGCAACTGGT GCAGCAGCAG 
ATCCAGCAGC AACAGCAACG CCAAAAGCTC TGGAACTTTG TGGATCCCAT GCAAAAGGCA 
CCCTGCATAT GCACCAAACA TCTGGGAAAC ACACCTCATG GAACACCACA CGGCGGCGCT 
TCAACGTACT CCCGCAACTC CTTGGGCGGC GATTCCTCGA TGCCGGTGGC CTCCGTGGAA 
TCACCGGCAA CGCCGGCTCC CTCTCCGCAT CCGAATTCGG CGCACTCGCA ACCGACATCC 
GTGCCGCCCG CTGAGCAACT GCTCAATATG AGTCCTCATG CGCCAACTTC CGTTTCCAAT 
CTCCAACAGC CGCCGACGCC TATCGACCAC CTGCTGGACA AGAATACACC GGCGCCAACG 
CCAACGGACC AGCACGACAG CAAGAGCATT ACGGCCTCGC CCTATGTTCA TCAGACGCCA 
AGCGTGGAGC CGCCCTCGTA TACGGATCAT GCGGCCGGCG GAGGGCCAGC TGGTGGGCAG 
GGCCTTGGTA CGGGTCCAGG TAGTGTGCCC GCCCAGCAAC CGGCTACGCC CACAGCAGCA 
ACGTCAGCTG GTGGCGCCGG ATCTGGCGGA CCTTCAAATG CAATTGGAGC AAATGCGGTT 
GGTACTATAA GTGTCAAGAA GCTTGAGATG CAGCAGCAGA CTCCATCGGC AGCTATGGCC 
ATCAAGCAGG AGCCTGGTGC TCAAGGTCGG GGGGTTGGAG GTGTTACCTC CACCACGGAG 
GCCTTGAACA ACTTTAAGCG ACTGTACAAC CCGCCAAAGC TGACGTTAAA GGATCCGGAC 
AGCTTCTACG ACGAGGAGTG GCTCAAGGAG GTCATCTATG ACTTTCAGTA CCAGGAATAC 
TGGGACTATA GCACGGTGAA ACGTCCGAAG ATGGAAAAGC AACGGAGACC TAGGTATGCC 
AAGAACCTTT ACGAAGGACA AAACCACGTG AAGCCCGTGA TGCCTTCGCC AGGATCCGTT 
TATGGCTCAC AGTTGCTTTC CTTGGATGAG TCAGCGAGCC AAGCAGGAGG GAGAGGCGGT 
GGAGGACAGG CGGCGGGCAG CTCCGGCGGT GGTCTAGTGG GCATTGCCAA CAGTACTGCC 
AGTGGCAGTG ATGTAGAAGC TGATGGTAGC AGCTTCTTCC AGGGACTGAA CATTAAGACA 
GAGCCTGGAT TGCATTCCCC TTCTTGCAAG GAAACGTCGA AATCCTCGGG CGGCAACAGC 
AGCGGTGGAG GAAGTGGTAG CGGTGGTAAT CTCTTCACAG CTGAAGGCTT GAATCCCTCG 
CTCAACGATC TGGAGCAGTT GTTTGAGACA AGCTCGAACG ACGAGTGCAG CAGTGTGCAA 
ATCCACACGC CACCCGATTC TAACAACCCC TCGAATGGCG GCTGCAGTGC TGTGACCAAT 
ACGATCGAAG ACCTCAAACG GAGCACAGCG GTGGCCAGTG CTGCCGTAGC AGCGGCAGCA 
GCAGCTGCGG CCTCGGGCGC GGGCAATATC CAGGCGGAGG ATCTTACCAA GATGTTTCCC 
ACACCACCGT CTCACGAGCA GCAGCACCCG AACTCGAGTC CCTGTCAAAC GGACGTGGTT 
ATGACGGATC TCAGTGTGGA CACCACCACA ACCATTATAA CCAGTAGTAT CACCACTACT 
TGCAATACCA CTATCACGAG TAGTATCAAC ACCACCACAA CAGCCTGTAG CAACCCAAGC 
AACAGTATTA TGCTGGCTGC CGCACAAGCT CCCGTCACCG TGGTGGCCAT CCAGACTGTC 
TCCAAAATGG TAAAGCAGGA GTATAACCTG GAGCTGGGTA GTCCTATGGA AGAGCCCATT 
AATGACTGGG ATTATGTGTA CCGACCTCCG CAACAGGAGA AGTTTGTGGG CTCGACGCGA 
TATGCTCCAT TGACCAACCT GCCCAGTCAG ACACAGCCGC CGCTGACTCT GCCTACGGGA 
TGCTTTTATC AACCCACCTG GAGTAGTCAC AAATCAAGAG CAGCAACTTT GGCTAAAGCA 
GCGGCAGCTC AACAGCAGCA GCATCAGAAA CATCAGGCCC TTCAGCAGCG CATCCAATTG 
CATCAGCAGA AACTACAGCA GCTCCAGCTT CAGAATCAGC AGCAACAACA AGCGGCAGCC 
GCAGCAGCAG CGGCGGCTAG TGGAGGAGTT GGCCACCAGA AGCACCAGCA TCAGCACCTT 
CATGATCTCC TGTCAGCGGC GCCGAGGACA CCATTAACGC CCTCTACTGT TCCGCAACCG 
TTGAGCAGTG GTGGCAGTCA GTATTTGCTG AATCAGTTGA ACTGCCCACA AGCGCCACCT 
GGTGCCTCCA TGCAGCAGCT AATGCACCGA GCGGGGATGT CACCAATTTC ACCGGGACCC 
GGAATGGGTC CGTATGCCGC CAGAAGTAGT CCAATGTCGA GGGCAACCCC CACGCATCCG 
CCACCGCCAT ATCCGTATGA CTTGGCTGTG GCCAGTCCAG CTACCTCCAC ATCATCGTAC 
TTGAACCGGC CGCTTCACTC GCAAGAGCAT CCGCACATGC ATGGCTTGGG CGGCGGAGCA 
ACGGGAGTTG TGGGTCACGG AACAGGAGGT GGCGGGCACA TGGGTATGGT AGCGTACACT 
GGCGATGCGG GCATTGTCAG TGGTGGAACA GCGATGGCAG CCGGATCCTC GTCCTTGCTT 
CAGGAATTAC CAGAAGTAAA TTCTGTCCTA GTTAACATCC TGCTGTACGA TACCGCCTTG 
AATGTCTTCC GGGATCACAA CTTTGATAGT AGCAGTGTGT GTGTGTGCAA TGCGGATACG 
CAAAAGATTG GCAATATTCG CGGAGCGGAT TCCGGAGTAT ATGTACCCCT GCCCGGGGTT 
AGCTTCAATC CTTTCCCTTC CGGAGCTGGA GGCGCTTTGG CTGGACAGCG AATGCTCAAT 
GGACCCAGTT CCGCCGGTTT TGGCGGCATG CGGATGATCA GTGCCTTTGG GGGTTCGCCA 
GCCTCAGCCT CGATGCCCGG AGCTGGCTCC GGACATGGCC ATGGTCCAAA TGGTGGCTCC 
AACTCGTCGT CTTGCACGCC GCCCAGCAGT AATCCCCATA TCACCGGGTA TGTGGACGAT 
GATCCCGTGG AGTGTACGTG TGGTTTCAGT GCAGTGGTTA ATCGAAGACT CTCTCATCGC 
GCTGGACTCT TTTACGAAGA TGAGGTGGAG ATCACGGGCA TCGCGGATGA TCCGGGCAGG 
AATAAGCAGC CCACGTTGCT CAGTATCATC CAGAGTCTTA GCAGGAAAAA CCAAAACAAG 
CAGGGACCTG GAGAAACAAG CTCTGCATTG GATAAAATTG GAGCGGGAGG ATTGCCTAAT 
GGACAACTGG AGCAATTGGG TCATGCTGTA TTTGACCTGC TATTGGATCA GTGCTCTATC 
ATCCAAACAT CTAGCAGTTC GGTGCACAGA GCACTTCAGT CTCATCGGAG GCGAATGTCC 
CGCCAACGAA GGATCTTCGG TAACAATGGA GCTCCCACTG CCTCATTGGC ATCAATTGCA 
AATGTTCTAG AGTTTATGGA TGCACACGAC GTTATAAGTT TGGCCCTGGA GCAGTCGAGA 
TTGGCATTCG AAAACCAGCG GATGGACAAC ATGATGGACT TCCATGGGAA TGGTAGCAGT 
AGCTCGCATC AGCAGCAGCA ACTGACAGCT TTCCATGCGC CACCACCTGC TTTGCGTCAC 
AAGCTAGCAG GTATTGGTGC TGGGCGGTTG ACGGTTCACA AGTGGCCATA TCTTCCCGTC 
GGTTTCACCC GTAGCAATAA GGAGATTGTA CGCACCATGA ATGCTATACA GCCGATGCTG 
CAGAATGCAT TCCATTGCAA ATCCAGGGGC GGCTCAGGTT CCAAGGATGC CAGTTCGTAC 
AATACGGTGA GTGGACCGCT AACCTGGCGC CAATTCCATC GACTGGCAGG CCGTGCATCC 
GGACAATGTG AGCCGCAACC GATACCATCC GTGGTGGTGG GCTACGAGAA GGATTGGATC 
TCGGTGGCAC CGCACTCCAT CCACTATTGG GATAAGTTTC TCCTGGAACC GTACTCCTAT 
GCCAGGGATG TTGTATACGT GGTAGTGTGT CCGGACAACG AGCATGTGGT AAATTGTACT 
CGCAGCTATT TCCGGGAACT AAGCAGCACC TACGAGATGT GCAAGCTGGG CAAACACACA 
CCCATCCGAG GATGGGATGG TTTCCTTCAA GTAGGCGCTG CTCGCAACAA CGTGCCTGCG 
GATCGGGAAA CAACTCCCTT GGATGATTGG CTGCGTACCC TGGAACATGC TGCTCTGGCG 
GAGCAAATCC GTCGGTATGC AGTTGCCTTT ATTCACCAGT TGGCTCCATA CCTAAGTCGA 
GTGCCAAATG ATAAAACGCT GTTGAATCCA CCAGATGGCA GTGGCAACTC ACACTCTAAA 
GGAGGAAGCT CTTGCTCCTC GAATAGCTCT TCAGTCAGCG GATTGCCAGG CGGGGACTTA 
CCCACGGATA ATATAAAGCT GGAGCCAGGT ACAGAACCAC AAGTCCAACC GATGGAGACT 
AACGAAATAA AACAGGAACC CGGAGTGGGA AAAGGAGGCA CTGCAGCAGG TGAAACCAAA 
CCAACTCTGA TCCTCGGAGA TCCGTTGGGA ATGGGCGAGA CTTTGGAGGA CATCAACCCG 
TCAGCCATTG TCCTTTATGT GGTCAATCCG TTCACTTTCG CCTCGGATAG CTGCGAGCTG 
GAGCGTCTAG CTCTGATTGC TCTACTTCGT TGCTATGCGG AACTGCTGAA AGCAGTCCCA 
GATTCAGTGC GATCCCAGAT GAACATACAG ATTATATCGC TAGAATCGGT AATGGAACTG 
GGTCCGTGCG GCAATCGAAA GCGTTTCTCG GACGAGATTA GGTGCCTGGC TCTAAACATA 
TTTTCGCAGT GCCGGCGACA TCTGGTGCAT GCCCAATCCG TTAAAAGTCT TACTGGCTTT 
GGTACGGCGG CTAATATGGA GGCGTTTCTC AAGACAAAGG ATGAACCCAA TCGCCGGGCC 
TACAAGATGT ACACGGCTCC CTTTGTTCTG GCACCAATGC ACGAGAGGAA TGACAAAACG 
GACTTCTCCA GATCAGCGGG AAGTATGCAT GGCCAGAATG AACATCGTTA TTCGGTTATG 
TATTGCAATT ACTGCCTAAG TGAGGATCAG GCTTGGCTTT TGGCCACCGC CACTGATGAA 
AGGGGCGAGA TGCTGGAGAA GATCTGCATT AATATTGATG TGCCAAATCG TGCTCGGAGG 
AGAAAAGCCC CTGCTCGCTA TGTGGCCCTA AAGAAACTGA TGGACTTCAT CATGGGAATC 
ATCTCGCAGA CATCGCAAAT GTGGCGTTTG GTTATCGGAC GCATTGGAAG GATCGGACAC 
AGTGAACTGA AGTCGTGGAG TTTTTTGCTC AGCAAGCAAC AGCTGCAAAA GGCATCCAAG 
CAATTTAAGG ATATGTGTAA GCAATGTACA TTGATGTATC CACCCACGAT CCTGAGTGCT 
TGCCTTGTGA CCTTGGAGCC GGATGCCAAG CTGCGCGTGA TGCCCGACCA GTTTACGCCA 
GATGAGCGCT TCTCACAGAT TTCCATGCAG AACCCGTTGG CCACTCCACA GGACGTGACC 
TGCACCCACA TCCTGGTCTT TCCCACTAGC GCCGTCTGTG CGCCTTTTAC GCGCCAGTTC 
CAAAACGAGC CGCAAGTAGA TGACGACTTT CTAACATTCG AGGAAGAGGG CAACGAGGAC 
TTCAGCGATG CGGACATTGG AGATCTCTTC TGGGACACTC ATATGGACAG AGTATCCAAT 
CATGGTAGTC CCGGTCGCAT GGATGACAAT CGGAGTTGGC AGAGCGCTGG CGGTAATAAT 
TTCAAGTGCA CGCCGCCCCA GGAAGTAGAG GAGGTTGGTT CACTCAACCA GCAGCCCATA 
TCAGTGGGTT ACATGGTGTC CACGGCGCCA ACCGGTCGCA TGCCTGCTTG GTTCTGGTCC 
GCCTGTCCGC ATCTAGAAGA CGTTTGTCCG GTGTTTCTGA AGACAGCCCT CCATCTCCAT 
GTGCCCAGCA TACAGTCTGC CGACGATATT CTCAACTCGA CCAATGCCCA TCAGTCAGGC 
AACGATCATC CGCTTGACTC CAATCTCACA GCGGATGTGC TGCGCTTCGT CCTCGAGGGA 
TACAATGCCC TTTCCTGGCT GGCGCTCGAC TCCAACACAC ACGATCGTCT CTCCTGTCTG 
CCCATCAATG TCCAGACGCT AATGGATCTG TACTATCTGA CGGCTGCAAT AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

skd ( NM_168879.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730590.1 Drosophila melanogaster skuld (skd), transcript variant C, mRNA.

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CloneID	OFa19333
Clone ID Related Accession (Same CDS sequence)	NM_079914.3 , NM_168879.3
Accession Version	NM_168879.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	skuld, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168879.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCATC AAAATCATCA GACAAACGGC GCAAGTTTGG AGGATTGCCA CACAAACTTT 
TATGCCTTGA CTGATTTATG TGGAATAAAG TGGCGGAAGT TCGTTAACGG GGAGCGACCG 
AACGCATCGA GCGATCCTTT GGCGGACCCG ATCCTGCGCT CATATTCGCG ATGCATCCAG 
GCGGACATGC TGTGCGTGTG GAGGAGGGTC CAATCCACGA AGACGGACCA CGCCGACCCG 
AATGCCTTAA CCTTCGAGAT GACCACGTCG ACCAAGGTCC ACCCGCCCCT TTCGCTGGCC 
GCCGCCAAGG AGCTATGGAT CTTCTGGTAC GGCGAGGAGC CCGATCTCAG TGAACTGGTC 
GATGCCGAGC TGCTCCGAGT AGCGGCCAAC CAAGCGCTTT GGAATGGCAC CTGGAAAGGA 
GCTCTCACCT ACGAGTGCCG GTCGCTGCTC TTTAAGGCGC TGCACAATCT CATGGAGAGA 
TTCGTGCTTA CCAAGGACAT TGTGCGCTTT GGAAAATGGT TTGTGCAGCC CTGCACCTCC 
AGCGATCGCC TCTTTGGACG CAGCTCCCAG CACTTGTCCT TCTCATTTAC GTTCTTTGTG 
CACGGCGACA CCGTTTGTGC CTCCATAGAT TTGCGCGAGC ATCCCGCCGT GCGTCCGCTG 
ACCAAGGAGC ACTTGACCGA AGCGGCGGCA GCCTTTGCAG CGGCCAGTTC TCCGCCAGGA 
TCAAATGGAT CTGCGGCGTC AGCAGGGGGA GCTGTGCCTA ATCCTGGTCA GGATCCAAAT 
GGAGCCAGCA TGGATGGACT GGACGGCGGA GAGGGAGCGG CCAAGGCAGC GCCACCTCCG 
CACGCGCGAA AGGTTATGCT GGCGCCCTTT GGAATTGCTG GCATACTTAC CGGCAATAGC 
TATAAGGCCA GCGATCCCAT AGCCGAGAAG ATCCTTGAGG ATTGGGCCTC GTTTTTTCCG 
CTGTGCAATA AGGATAACAC GGATGTGCCA CCCGTGGTGG AAGTGGTGTC GGGTGGTCAT 
AAGATGTATC ATCCCACGAA CTACGTACTA GTCACAGACC TGGACGACAT GGAGCACATG 
GAATTCGTCG AGATGCAGAA GATGCAAAGC TCAGTGGCCG GAGCGGCGGC CGAGTCAGCT 
GTTCTGGCCT CGCTTTCCTG CCCACCGGGA GCAGCATCTG CTCCTTCATC GATGGGAGCA 
GCTCCATCTG CAACAGCGGT TCCCTTGGGT CCAGCTTCCC TCAATGCGCC AGCTTTAGCA 
GGAGCAGGTG TCGGAGCCAC CGCATCTTCC GCCGCCAAAG AGATATCCCG CAAGGCAGCT 
CCACAAGCGG TTAGCGCCCT GGAACGTCTC GCCTTCCAGC CCTACTACGA TCAACGGCCC 
ACCTCGGGCT TCACCTTCAA TACCAACAAT ACTCACATAC CCGCCTCGGC AGCCGTGGAA 
ATGCCGGAAC GAACCTGGCA GGATTGCGTG ATGAACACGC TCCATGTGGA TGCAGCGGCG 
GCGGCAGCAG CAGTTGCTTC GTCTACGCCC GCCTCGGGAA CTGGGTCATT ATCCGCGGAT 
GGGGACGAGA ATGAGCAGAA TAAACCGCCA CAGGACTCCA AGCAACTGGT GCAGCAGCAG 
ATCCAGCAGC AACAGCAACG CCAAAAGCTC TGGAACTTTG TGGATCCCAT GCAAAAGGCA 
CCCTGCATAT GCACCAAACA TCTGGGAAAC ACACCTCATG GAACACCACA CGGCGGCGCT 
TCAACGTACT CCCGCAACTC CTTGGGCGGC GATTCCTCGA TGCCGGTGGC CTCCGTGGAA 
TCACCGGCAA CGCCGGCTCC CTCTCCGCAT CCGAATTCGG CGCACTCGCA ACCGACATCC 
GTGCCGCCCG CTGAGCAACT GCTCAATATG AGTCCTCATG CGCCAACTTC CGTTTCCAAT 
CTCCAACAGC CGCCGACGCC TATCGACCAC CTGCTGGACA AGAATACACC GGCGCCAACG 
CCAACGGACC AGCACGACAG CAAGAGCATT ACGGCCTCGC CCTATGTTCA TCAGACGCCA 
AGCGTGGAGC CGCCCTCGTA TACGGATCAT GCGGCCGGCG GAGGGCCAGC TGGTGGGCAG 
GGCCTTGGTA CGGGTCCAGG TAGTGTGCCC GCCCAGCAAC CGGCTACGCC CACAGCAGCA 
ACGTCAGCTG GTGGCGCCGG ATCTGGCGGA CCTTCAAATG CAATTGGAGC AAATGCGGTT 
GGTACTATAA GTGTCAAGAA GCTTGAGATG CAGCAGCAGA CTCCATCGGC AGCTATGGCC 
ATCAAGCAGG AGCCTGGTGC TCAAGGTCGG GGGGTTGGAG GTGTTACCTC CACCACGGAG 
GCCTTGAACA ACTTTAAGCG ACTGTACAAC CCGCCAAAGC TGACGTTAAA GGATCCGGAC 
AGCTTCTACG ACGAGGAGTG GCTCAAGGAG GTCATCTATG ACTTTCAGTA CCAGGAATAC 
TGGGACTATA GCACGGTGAA ACGTCCGAAG ATGGAAAAGC AACGGAGACC TAGGTATGCC 
AAGAACCTTT ACGAAGGACA AAACCACGTG AAGCCCGTGA TGCCTTCGCC AGGATCCGTT 
TATGGCTCAC AGTTGCTTTC CTTGGATGAG TCAGCGAGCC AAGCAGGAGG GAGAGGCGGT 
GGAGGACAGG CGGCGGGCAG CTCCGGCGGT GGTCTAGTGG GCATTGCCAA CAGTACTGCC 
AGTGGCAGTG ATGTAGAAGC TGATGGTAGC AGCTTCTTCC AGGGACTGAA CATTAAGACA 
GAGCCTGGAT TGCATTCCCC TTCTTGCAAG GAAACGTCGA AATCCTCGGG CGGCAACAGC 
AGCGGTGGAG GAAGTGGTAG CGGTGGTAAT CTCTTCACAG CTGAAGGCTT GAATCCCTCG 
CTCAACGATC TGGAGCAGTT GTTTGAGACA AGCTCGAACG ACGAGTGCAG CAGTGTGCAA 
ATCCACACGC CACCCGATTC TAACAACCCC TCGAATGGCG GCTGCAGTGC TGTGACCAAT 
ACGATCGAAG ACCTCAAACG GAGCACAGCG GTGGCCAGTG CTGCCGTAGC AGCGGCAGCA 
GCAGCTGCGG CCTCGGGCGC GGGCAATATC CAGGCGGAGG ATCTTACCAA GATGTTTCCC 
ACACCACCGT CTCACGAGCA GCAGCACCCG AACTCGAGTC CCTGTCAAAC GGACGTGGTT 
ATGACGGATC TCAGTGTGGA CACCACCACA ACCATTATAA CCAGTAGTAT CACCACTACT 
TGCAATACCA CTATCACGAG TAGTATCAAC ACCACCACAA CAGCCTGTAG CAACCCAAGC 
AACAGTATTA TGCTGGCTGC CGCACAAGCT CCCGTCACCG TGGTGGCCAT CCAGACTGTC 
TCCAAAATGG TAAAGCAGGA GTATAACCTG GAGCTGGGTA GTCCTATGGA AGAGCCCATT 
AATGACTGGG ATTATGTGTA CCGACCTCCG CAACAGGAGA AGTTTGTGGG CTCGACGCGA 
TATGCTCCAT TGACCAACCT GCCCAGTCAG ACACAGCCGC CGCTGACTCT GCCTACGGGA 
TGCTTTTATC AACCCACCTG GAGTAGTCAC AAATCAAGAG CAGCAACTTT GGCTAAAGCA 
GCGGCAGCTC AACAGCAGCA GCATCAGAAA CATCAGGCCC TTCAGCAGCG CATCCAATTG 
CATCAGCAGA AACTACAGCA GCTCCAGCTT CAGAATCAGC AGCAACAACA AGCGGCAGCC 
GCAGCAGCAG CGGCGGCTAG TGGAGGAGTT GGCCACCAGA AGCACCAGCA TCAGCACCTT 
CATGATCTCC TGTCAGCGGC GCCGAGGACA CCATTAACGC CCTCTACTGT TCCGCAACCG 
TTGAGCAGTG GTGGCAGTCA GTATTTGCTG AATCAGTTGA ACTGCCCACA AGCGCCACCT 
GGTGCCTCCA TGCAGCAGCT AATGCACCGA GCGGGGATGT CACCAATTTC ACCGGGACCC 
GGAATGGGTC CGTATGCCGC CAGAAGTAGT CCAATGTCGA GGGCAACCCC CACGCATCCG 
CCACCGCCAT ATCCGTATGA CTTGGCTGTG GCCAGTCCAG CTACCTCCAC ATCATCGTAC 
TTGAACCGGC CGCTTCACTC GCAAGAGCAT CCGCACATGC ATGGCTTGGG CGGCGGAGCA 
ACGGGAGTTG TGGGTCACGG AACAGGAGGT GGCGGGCACA TGGGTATGGT AGCGTACACT 
GGCGATGCGG GCATTGTCAG TGGTGGAACA GCGATGGCAG CCGGATCCTC GTCCTTGCTT 
CAGGAATTAC CAGAAGTAAA TTCTGTCCTA GTTAACATCC TGCTGTACGA TACCGCCTTG 
AATGTCTTCC GGGATCACAA CTTTGATAGT AGCAGTGTGT GTGTGTGCAA TGCGGATACG 
CAAAAGATTG GCAATATTCG CGGAGCGGAT TCCGGAGTAT ATGTACCCCT GCCCGGGGTT 
AGCTTCAATC CTTTCCCTTC CGGAGCTGGA GGCGCTTTGG CTGGACAGCG AATGCTCAAT 
GGACCCAGTT CCGCCGGTTT TGGCGGCATG CGGATGATCA GTGCCTTTGG GGGTTCGCCA 
GCCTCAGCCT CGATGCCCGG AGCTGGCTCC GGACATGGCC ATGGTCCAAA TGGTGGCTCC 
AACTCGTCGT CTTGCACGCC GCCCAGCAGT AATCCCCATA TCACCGGGTA TGTGGACGAT 
GATCCCGTGG AGTGTACGTG TGGTTTCAGT GCAGTGGTTA ATCGAAGACT CTCTCATCGC 
GCTGGACTCT TTTACGAAGA TGAGGTGGAG ATCACGGGCA TCGCGGATGA TCCGGGCAGG 
AATAAGCAGC CCACGTTGCT CAGTATCATC CAGAGTCTTA GCAGGAAAAA CCAAAACAAG 
CAGGGACCTG GAGAAACAAG CTCTGCATTG GATAAAATTG GAGCGGGAGG ATTGCCTAAT 
GGACAACTGG AGCAATTGGG TCATGCTGTA TTTGACCTGC TATTGGATCA GTGCTCTATC 
ATCCAAACAT CTAGCAGTTC GGTGCACAGA GCACTTCAGT CTCATCGGAG GCGAATGTCC 
CGCCAACGAA GGATCTTCGG TAACAATGGA GCTCCCACTG CCTCATTGGC ATCAATTGCA 
AATGTTCTAG AGTTTATGGA TGCACACGAC GTTATAAGTT TGGCCCTGGA GCAGTCGAGA 
TTGGCATTCG AAAACCAGCG GATGGACAAC ATGATGGACT TCCATGGGAA TGGTAGCAGT 
AGCTCGCATC AGCAGCAGCA ACTGACAGCT TTCCATGCGC CACCACCTGC TTTGCGTCAC 
AAGCTAGCAG GTATTGGTGC TGGGCGGTTG ACGGTTCACA AGTGGCCATA TCTTCCCGTC 
GGTTTCACCC GTAGCAATAA GGAGATTGTA CGCACCATGA ATGCTATACA GCCGATGCTG 
CAGAATGCAT TCCATTGCAA ATCCAGGGGC GGCTCAGGTT CCAAGGATGC CAGTTCGTAC 
AATACGGTGA GTGGACCGCT AACCTGGCGC CAATTCCATC GACTGGCAGG CCGTGCATCC 
GGACAATGTG AGCCGCAACC GATACCATCC GTGGTGGTGG GCTACGAGAA GGATTGGATC 
TCGGTGGCAC CGCACTCCAT CCACTATTGG GATAAGTTTC TCCTGGAACC GTACTCCTAT 
GCCAGGGATG TTGTATACGT GGTAGTGTGT CCGGACAACG AGCATGTGGT AAATTGTACT 
CGCAGCTATT TCCGGGAACT AAGCAGCACC TACGAGATGT GCAAGCTGGG CAAACACACA 
CCCATCCGAG GATGGGATGG TTTCCTTCAA GTAGGCGCTG CTCGCAACAA CGTGCCTGCG 
GATCGGGAAA CAACTCCCTT GGATGATTGG CTGCGTACCC TGGAACATGC TGCTCTGGCG 
GAGCAAATCC GTCGGTATGC AGTTGCCTTT ATTCACCAGT TGGCTCCATA CCTAAGTCGA 
GTGCCAAATG ATAAAACGCT GTTGAATCCA CCAGATGGCA GTGGCAACTC ACACTCTAAA 
GGAGGAAGCT CTTGCTCCTC GAATAGCTCT TCAGTCAGCG GATTGCCAGG CGGGGACTTA 
CCCACGGATA ATATAAAGCT GGAGCCAGGT ACAGAACCAC AAGTCCAACC GATGGAGACT 
AACGAAATAA AACAGGAACC CGGAGTGGGA AAAGGAGGCA CTGCAGCAGG TGAAACCAAA 
CCAACTCTGA TCCTCGGAGA TCCGTTGGGA ATGGGCGAGA CTTTGGAGGA CATCAACCCG 
TCAGCCATTG TCCTTTATGT GGTCAATCCG TTCACTTTCG CCTCGGATAG CTGCGAGCTG 
GAGCGTCTAG CTCTGATTGC TCTACTTCGT TGCTATGCGG AACTGCTGAA AGCAGTCCCA 
GATTCAGTGC GATCCCAGAT GAACATACAG ATTATATCGC TAGAATCGGT AATGGAACTG 
GGTCCGTGCG GCAATCGAAA GCGTTTCTCG GACGAGATTA GGTGCCTGGC TCTAAACATA 
TTTTCGCAGT GCCGGCGACA TCTGGTGCAT GCCCAATCCG TTAAAAGTCT TACTGGCTTT 
GGTACGGCGG CTAATATGGA GGCGTTTCTC AAGACAAAGG ATGAACCCAA TCGCCGGGCC 
TACAAGATGT ACACGGCTCC CTTTGTTCTG GCACCAATGC ACGAGAGGAA TGACAAAACG 
GACTTCTCCA GATCAGCGGG AAGTATGCAT GGCCAGAATG AACATCGTTA TTCGGTTATG 
TATTGCAATT ACTGCCTAAG TGAGGATCAG GCTTGGCTTT TGGCCACCGC CACTGATGAA 
AGGGGCGAGA TGCTGGAGAA GATCTGCATT AATATTGATG TGCCAAATCG TGCTCGGAGG 
AGAAAAGCCC CTGCTCGCTA TGTGGCCCTA AAGAAACTGA TGGACTTCAT CATGGGAATC 
ATCTCGCAGA CATCGCAAAT GTGGCGTTTG GTTATCGGAC GCATTGGAAG GATCGGACAC 
AGTGAACTGA AGTCGTGGAG TTTTTTGCTC AGCAAGCAAC AGCTGCAAAA GGCATCCAAG 
CAATTTAAGG ATATGTGTAA GCAATGTACA TTGATGTATC CACCCACGAT CCTGAGTGCT 
TGCCTTGTGA CCTTGGAGCC GGATGCCAAG CTGCGCGTGA TGCCCGACCA GTTTACGCCA 
GATGAGCGCT TCTCACAGAT TTCCATGCAG AACCCGTTGG CCACTCCACA GGACGTGACC 
TGCACCCACA TCCTGGTCTT TCCCACTAGC GCCGTCTGTG CGCCTTTTAC GCGCCAGTTC 
CAAAACGAGC CGCAAGTAGA TGACGACTTT CTAACATTCG AGGAAGAGGG CAACGAGGAC 
TTCAGCGATG CGGACATTGG AGATCTCTTC TGGGACACTC ATATGGACAG AGTATCCAAT 
CATGGTAGTC CCGGTCGCAT GGATGACAAT CGGAGTTGGC AGAGCGCTGG CGGTAATAAT 
TTCAAGTGCA CGCCGCCCCA GGAAGTAGAG GAGGTTGGTT CACTCAACCA GCAGCCCATA 
TCAGTGGGTT ACATGGTGTC CACGGCGCCA ACCGGTCGCA TGCCTGCTTG GTTCTGGTCC 
GCCTGTCCGC ATCTAGAAGA CGTTTGTCCG GTGTTTCTGA AGACAGCCCT CCATCTCCAT 
GTGCCCAGCA TACAGTCTGC CGACGATATT CTCAACTCGA CCAATGCCCA TCAGTCAGGC 
AACGATCATC CGCTTGACTC CAATCTCACA GCGGATGTGC TGCGCTTCGT CCTCGAGGGA 
TACAATGCCC TTTCCTGGCT GGCGCTCGAC TCCAACACAC ACGATCGTCT CTCCTGTCTG 
CCCATCAATG TCCAGACGCT AATGGATCTG TACTATCTGA CGGCTGCAAT AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730590.1
CDS	668..8524
Translation	MTHQNHQTNGASLEDCHTNFYALTDLCGIKWRKFVNGERPNASSDPLADPILRSYSRCIQADMLCVWRRVQSTKTDHADPNALTFEMTTSTKVHPPLSLAAAKELWIFWYGEEPDLSELVDAELLRVAANQALWNGTWKGALTYECRSLLFKALHNLMERFVLTKDIVRFGKWFVQPCTSSDRLFGRSSQHLSFSFTFFVHGDTVCASIDLREHPAVRPLTKEHLTEAAAAFAAASSPPGSNGSAASAGGAVPNPGQDPNGASMDGLDGGEGAAKAAPPPHARKVMLAPFGIAGILTGNSYKASDPIAEKILEDWASFFPLCNKDNTDVPPVVEVVSGGHKMYHPTNYVLVTDLDDMEHMEFVEMQKMQSSVAGAAAESAVLASLSCPPGAASAPSSMGAAPSATAVPLGPASLNAPALAGAGVGATASSAAKEISRKAAPQAVSALERLAFQPYYDQRPTSGFTFNTNNTHIPASAAVEMPERTWQDCVMNTLHVDAAAAAAAVASSTPASGTGSLSADGDENEQNKPPQDSKQLVQQQIQQQQQRQKLWNFVDPMQKAPCICTKHLGNTPHGTPHGGASTYSRNSLGGDSSMPVASVESPATPAPSPHPNSAHSQPTSVPPAEQLLNMSPHAPTSVSNLQQPPTPIDHLLDKNTPAPTPTDQHDSKSITASPYVHQTPSVEPPSYTDHAAGGGPAGGQGLGTGPGSVPAQQPATPTAATSAGGAGSGGPSNAIGANAVGTISVKKLEMQQQTPSAAMAIKQEPGAQGRGVGGVTSTTEALNNFKRLYNPPKLTLKDPDSFYDEEWLKEVIYDFQYQEYWDYSTVKRPKMEKQRRPRYAKNLYEGQNHVKPVMPSPGSVYGSQLLSLDESASQAGGRGGGGQAAGSSGGGLVGIANSTASGSDVEADGSSFFQGLNIKTEPGLHSPSCKETSKSSGGNSSGGGSGSGGNLFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSAVTNTIEDLKRSTAVASAAVAAAAAAAASGAGNIQAEDLTKMFPTPPSHEQQHPNSSPCQTDVVMTDLSVDTTTTIITSSITTTCNTTITSSINTTTTACSNPSNSIMLAAAQAPVTVVAIQTVSKMVKQEYNLELGSPMEEPINDWDYVYRPPQQEKFVGSTRYAPLTNLPSQTQPPLTLPTGCFYQPTWSSHKSRAATLAKAAAAQQQQHQKHQALQQRIQLHQQKLQQLQLQNQQQQQAAAAAAAAASGGVGHQKHQHQHLHDLLSAAPRTPLTPSTVPQPLSSGGSQYLLNQLNCPQAPPGASMQQLMHRAGMSPISPGPGMGPYAARSSPMSRATPTHPPPPYPYDLAVASPATSTSSYLNRPLHSQEHPHMHGLGGGATGVVGHGTGGGGHMGMVAYTGDAGIVSGGTAMAAGSSSLLQELPEVNSVLVNILLYDTALNVFRDHNFDSSSVCVCNADTQKIGNIRGADSGVYVPLPGVSFNPFPSGAGGALAGQRMLNGPSSAGFGGMRMISAFGGSPASASMPGAGSGHGHGPNGGSNSSSCTPPSSNPHITGYVDDDPVECTCGFSAVVNRRLSHRAGLFYEDEVEITGIADDPGRNKQPTLLSIIQSLSRKNQNKQGPGETSSALDKIGAGGLPNGQLEQLGHAVFDLLLDQCSIIQTSSSSVHRALQSHRRRMSRQRRIFGNNGAPTASLASIANVLEFMDAHDVISLALEQSRLAFENQRMDNMMDFHGNGSSSSHQQQQLTAFHAPPPALRHKLAGIGAGRLTVHKWPYLPVGFTRSNKEIVRTMNAIQPMLQNAFHCKSRGGSGSKDASSYNTVSGPLTWRQFHRLAGRASGQCEPQPIPSVVVGYEKDWISVAPHSIHYWDKFLLEPYSYARDVVYVVVCPDNEHVVNCTRSYFRELSSTYEMCKLGKHTPIRGWDGFLQVGAARNNVPADRETTPLDDWLRTLEHAALAEQIRRYAVAFIHQLAPYLSRVPNDKTLLNPPDGSGNSHSKGGSSCSSNSSSVSGLPGGDLPTDNIKLEPGTEPQVQPMETNEIKQEPGVGKGGTAAGETKPTLILGDPLGMGETLEDINPSAIVLYVVNPFTFASDSCELERLALIALLRCYAELLKAVPDSVRSQMNIQIISLESVMELGPCGNRKRFSDEIRCLALNIFSQCRRHLVHAQSVKSLTGFGTAANMEAFLKTKDEPNRRAYKMYTAPFVLAPMHERNDKTDFSRSAGSMHGQNEHRYSVMYCNYCLSEDQAWLLATATDERGEMLEKICINIDVPNRARRRKAPARYVALKKLMDFIMGIISQTSQMWRLVIGRIGRIGHSELKSWSFLLSKQQLQKASKQFKDMCKQCTLMYPPTILSACLVTLEPDAKLRVMPDQFTPDERFSQISMQNPLATPQDVTCTHILVFPTSAVCAPFTRQFQNEPQVDDDFLTFEEEGNEDFSDADIGDLFWDTHMDRVSNHGSPGRMDDNRSWQSAGGNNFKCTPPQEVEEVGSLNQQPISVGYMVSTAPTGRMPAWFWSACPHLEDVCPVFLKTALHLHVPSIQSADDILNSTNAHQSGNDHPLDSNLTADVLRFVLEGYNALSWLALDSNTHDRLSCLPINVQTLMDLYYLTAAIA

Target ORF information:

RefSeq Version	NM_168879.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster skuld (skd), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168879.3

ORF Insert Sequence:

ATGACGCATC AAAATCATCA GACAAACGGC GCAAGTTTGG AGGATTGCCA CACAAACTTT 
TATGCCTTGA CTGATTTATG TGGAATAAAG TGGCGGAAGT TCGTTAACGG GGAGCGACCG 
AACGCATCGA GCGATCCTTT GGCGGACCCG ATCCTGCGCT CATATTCGCG ATGCATCCAG 
GCGGACATGC TGTGCGTGTG GAGGAGGGTC CAATCCACGA AGACGGACCA CGCCGACCCG 
AATGCCTTAA CCTTCGAGAT GACCACGTCG ACCAAGGTCC ACCCGCCCCT TTCGCTGGCC 
GCCGCCAAGG AGCTATGGAT CTTCTGGTAC GGCGAGGAGC CCGATCTCAG TGAACTGGTC 
GATGCCGAGC TGCTCCGAGT AGCGGCCAAC CAAGCGCTTT GGAATGGCAC CTGGAAAGGA 
GCTCTCACCT ACGAGTGCCG GTCGCTGCTC TTTAAGGCGC TGCACAATCT CATGGAGAGA 
TTCGTGCTTA CCAAGGACAT TGTGCGCTTT GGAAAATGGT TTGTGCAGCC CTGCACCTCC 
AGCGATCGCC TCTTTGGACG CAGCTCCCAG CACTTGTCCT TCTCATTTAC GTTCTTTGTG 
CACGGCGACA CCGTTTGTGC CTCCATAGAT TTGCGCGAGC ATCCCGCCGT GCGTCCGCTG 
ACCAAGGAGC ACTTGACCGA AGCGGCGGCA GCCTTTGCAG CGGCCAGTTC TCCGCCAGGA 
TCAAATGGAT CTGCGGCGTC AGCAGGGGGA GCTGTGCCTA ATCCTGGTCA GGATCCAAAT 
GGAGCCAGCA TGGATGGACT GGACGGCGGA GAGGGAGCGG CCAAGGCAGC GCCACCTCCG 
CACGCGCGAA AGGTTATGCT GGCGCCCTTT GGAATTGCTG GCATACTTAC CGGCAATAGC 
TATAAGGCCA GCGATCCCAT AGCCGAGAAG ATCCTTGAGG ATTGGGCCTC GTTTTTTCCG 
CTGTGCAATA AGGATAACAC GGATGTGCCA CCCGTGGTGG AAGTGGTGTC GGGTGGTCAT 
AAGATGTATC ATCCCACGAA CTACGTACTA GTCACAGACC TGGACGACAT GGAGCACATG 
GAATTCGTCG AGATGCAGAA GATGCAAAGC TCAGTGGCCG GAGCGGCGGC CGAGTCAGCT 
GTTCTGGCCT CGCTTTCCTG CCCACCGGGA GCAGCATCTG CTCCTTCATC GATGGGAGCA 
GCTCCATCTG CAACAGCGGT TCCCTTGGGT CCAGCTTCCC TCAATGCGCC AGCTTTAGCA 
GGAGCAGGTG TCGGAGCCAC CGCATCTTCC GCCGCCAAAG AGATATCCCG CAAGGCAGCT 
CCACAAGCGG TTAGCGCCCT GGAACGTCTC GCCTTCCAGC CCTACTACGA TCAACGGCCC 
ACCTCGGGCT TCACCTTCAA TACCAACAAT ACTCACATAC CCGCCTCGGC AGCCGTGGAA 
ATGCCGGAAC GAACCTGGCA GGATTGCGTG ATGAACACGC TCCATGTGGA TGCAGCGGCG 
GCGGCAGCAG CAGTTGCTTC GTCTACGCCC GCCTCGGGAA CTGGGTCATT ATCCGCGGAT 
GGGGACGAGA ATGAGCAGAA TAAACCGCCA CAGGACTCCA AGCAACTGGT GCAGCAGCAG 
ATCCAGCAGC AACAGCAACG CCAAAAGCTC TGGAACTTTG TGGATCCCAT GCAAAAGGCA 
CCCTGCATAT GCACCAAACA TCTGGGAAAC ACACCTCATG GAACACCACA CGGCGGCGCT 
TCAACGTACT CCCGCAACTC CTTGGGCGGC GATTCCTCGA TGCCGGTGGC CTCCGTGGAA 
TCACCGGCAA CGCCGGCTCC CTCTCCGCAT CCGAATTCGG CGCACTCGCA ACCGACATCC 
GTGCCGCCCG CTGAGCAACT GCTCAATATG AGTCCTCATG CGCCAACTTC CGTTTCCAAT 
CTCCAACAGC CGCCGACGCC TATCGACCAC CTGCTGGACA AGAATACACC GGCGCCAACG 
CCAACGGACC AGCACGACAG CAAGAGCATT ACGGCCTCGC CCTATGTTCA TCAGACGCCA 
AGCGTGGAGC CGCCCTCGTA TACGGATCAT GCGGCCGGCG GAGGGCCAGC TGGTGGGCAG 
GGCCTTGGTA CGGGTCCAGG TAGTGTGCCC GCCCAGCAAC CGGCTACGCC CACAGCAGCA 
ACGTCAGCTG GTGGCGCCGG ATCTGGCGGA CCTTCAAATG CAATTGGAGC AAATGCGGTT 
GGTACTATAA GTGTCAAGAA GCTTGAGATG CAGCAGCAGA CTCCATCGGC AGCTATGGCC 
ATCAAGCAGG AGCCTGGTGC TCAAGGTCGG GGGGTTGGAG GTGTTACCTC CACCACGGAG 
GCCTTGAACA ACTTTAAGCG ACTGTACAAC CCGCCAAAGC TGACGTTAAA GGATCCGGAC 
AGCTTCTACG ACGAGGAGTG GCTCAAGGAG GTCATCTATG ACTTTCAGTA CCAGGAATAC 
TGGGACTATA GCACGGTGAA ACGTCCGAAG ATGGAAAAGC AACGGAGACC TAGGTATGCC 
AAGAACCTTT ACGAAGGACA AAACCACGTG AAGCCCGTGA TGCCTTCGCC AGGATCCGTT 
TATGGCTCAC AGTTGCTTTC CTTGGATGAG TCAGCGAGCC AAGCAGGAGG GAGAGGCGGT 
GGAGGACAGG CGGCGGGCAG CTCCGGCGGT GGTCTAGTGG GCATTGCCAA CAGTACTGCC 
AGTGGCAGTG ATGTAGAAGC TGATGGTAGC AGCTTCTTCC AGGGACTGAA CATTAAGACA 
GAGCCTGGAT TGCATTCCCC TTCTTGCAAG GAAACGTCGA AATCCTCGGG CGGCAACAGC 
AGCGGTGGAG GAAGTGGTAG CGGTGGTAAT CTCTTCACAG CTGAAGGCTT GAATCCCTCG 
CTCAACGATC TGGAGCAGTT GTTTGAGACA AGCTCGAACG ACGAGTGCAG CAGTGTGCAA 
ATCCACACGC CACCCGATTC TAACAACCCC TCGAATGGCG GCTGCAGTGC TGTGACCAAT 
ACGATCGAAG ACCTCAAACG GAGCACAGCG GTGGCCAGTG CTGCCGTAGC AGCGGCAGCA 
GCAGCTGCGG CCTCGGGCGC GGGCAATATC CAGGCGGAGG ATCTTACCAA GATGTTTCCC 
ACACCACCGT CTCACGAGCA GCAGCACCCG AACTCGAGTC CCTGTCAAAC GGACGTGGTT 
ATGACGGATC TCAGTGTGGA CACCACCACA ACCATTATAA CCAGTAGTAT CACCACTACT 
TGCAATACCA CTATCACGAG TAGTATCAAC ACCACCACAA CAGCCTGTAG CAACCCAAGC 
AACAGTATTA TGCTGGCTGC CGCACAAGCT CCCGTCACCG TGGTGGCCAT CCAGACTGTC 
TCCAAAATGG TAAAGCAGGA GTATAACCTG GAGCTGGGTA GTCCTATGGA AGAGCCCATT 
AATGACTGGG ATTATGTGTA CCGACCTCCG CAACAGGAGA AGTTTGTGGG CTCGACGCGA 
TATGCTCCAT TGACCAACCT GCCCAGTCAG ACACAGCCGC CGCTGACTCT GCCTACGGGA 
TGCTTTTATC AACCCACCTG GAGTAGTCAC AAATCAAGAG CAGCAACTTT GGCTAAAGCA 
GCGGCAGCTC AACAGCAGCA GCATCAGAAA CATCAGGCCC TTCAGCAGCG CATCCAATTG 
CATCAGCAGA AACTACAGCA GCTCCAGCTT CAGAATCAGC AGCAACAACA AGCGGCAGCC 
GCAGCAGCAG CGGCGGCTAG TGGAGGAGTT GGCCACCAGA AGCACCAGCA TCAGCACCTT 
CATGATCTCC TGTCAGCGGC GCCGAGGACA CCATTAACGC CCTCTACTGT TCCGCAACCG 
TTGAGCAGTG GTGGCAGTCA GTATTTGCTG AATCAGTTGA ACTGCCCACA AGCGCCACCT 
GGTGCCTCCA TGCAGCAGCT AATGCACCGA GCGGGGATGT CACCAATTTC ACCGGGACCC 
GGAATGGGTC CGTATGCCGC CAGAAGTAGT CCAATGTCGA GGGCAACCCC CACGCATCCG 
CCACCGCCAT ATCCGTATGA CTTGGCTGTG GCCAGTCCAG CTACCTCCAC ATCATCGTAC 
TTGAACCGGC CGCTTCACTC GCAAGAGCAT CCGCACATGC ATGGCTTGGG CGGCGGAGCA 
ACGGGAGTTG TGGGTCACGG AACAGGAGGT GGCGGGCACA TGGGTATGGT AGCGTACACT 
GGCGATGCGG GCATTGTCAG TGGTGGAACA GCGATGGCAG CCGGATCCTC GTCCTTGCTT 
CAGGAATTAC CAGAAGTAAA TTCTGTCCTA GTTAACATCC TGCTGTACGA TACCGCCTTG 
AATGTCTTCC GGGATCACAA CTTTGATAGT AGCAGTGTGT GTGTGTGCAA TGCGGATACG 
CAAAAGATTG GCAATATTCG CGGAGCGGAT TCCGGAGTAT ATGTACCCCT GCCCGGGGTT 
AGCTTCAATC CTTTCCCTTC CGGAGCTGGA GGCGCTTTGG CTGGACAGCG AATGCTCAAT 
GGACCCAGTT CCGCCGGTTT TGGCGGCATG CGGATGATCA GTGCCTTTGG GGGTTCGCCA 
GCCTCAGCCT CGATGCCCGG AGCTGGCTCC GGACATGGCC ATGGTCCAAA TGGTGGCTCC 
AACTCGTCGT CTTGCACGCC GCCCAGCAGT AATCCCCATA TCACCGGGTA TGTGGACGAT 
GATCCCGTGG AGTGTACGTG TGGTTTCAGT GCAGTGGTTA ATCGAAGACT CTCTCATCGC 
GCTGGACTCT TTTACGAAGA TGAGGTGGAG ATCACGGGCA TCGCGGATGA TCCGGGCAGG 
AATAAGCAGC CCACGTTGCT CAGTATCATC CAGAGTCTTA GCAGGAAAAA CCAAAACAAG 
CAGGGACCTG GAGAAACAAG CTCTGCATTG GATAAAATTG GAGCGGGAGG ATTGCCTAAT 
GGACAACTGG AGCAATTGGG TCATGCTGTA TTTGACCTGC TATTGGATCA GTGCTCTATC 
ATCCAAACAT CTAGCAGTTC GGTGCACAGA GCACTTCAGT CTCATCGGAG GCGAATGTCC 
CGCCAACGAA GGATCTTCGG TAACAATGGA GCTCCCACTG CCTCATTGGC ATCAATTGCA 
AATGTTCTAG AGTTTATGGA TGCACACGAC GTTATAAGTT TGGCCCTGGA GCAGTCGAGA 
TTGGCATTCG AAAACCAGCG GATGGACAAC ATGATGGACT TCCATGGGAA TGGTAGCAGT 
AGCTCGCATC AGCAGCAGCA ACTGACAGCT TTCCATGCGC CACCACCTGC TTTGCGTCAC 
AAGCTAGCAG GTATTGGTGC TGGGCGGTTG ACGGTTCACA AGTGGCCATA TCTTCCCGTC 
GGTTTCACCC GTAGCAATAA GGAGATTGTA CGCACCATGA ATGCTATACA GCCGATGCTG 
CAGAATGCAT TCCATTGCAA ATCCAGGGGC GGCTCAGGTT CCAAGGATGC CAGTTCGTAC 
AATACGGTGA GTGGACCGCT AACCTGGCGC CAATTCCATC GACTGGCAGG CCGTGCATCC 
GGACAATGTG AGCCGCAACC GATACCATCC GTGGTGGTGG GCTACGAGAA GGATTGGATC 
TCGGTGGCAC CGCACTCCAT CCACTATTGG GATAAGTTTC TCCTGGAACC GTACTCCTAT 
GCCAGGGATG TTGTATACGT GGTAGTGTGT CCGGACAACG AGCATGTGGT AAATTGTACT 
CGCAGCTATT TCCGGGAACT AAGCAGCACC TACGAGATGT GCAAGCTGGG CAAACACACA 
CCCATCCGAG GATGGGATGG TTTCCTTCAA GTAGGCGCTG CTCGCAACAA CGTGCCTGCG 
GATCGGGAAA CAACTCCCTT GGATGATTGG CTGCGTACCC TGGAACATGC TGCTCTGGCG 
GAGCAAATCC GTCGGTATGC AGTTGCCTTT ATTCACCAGT TGGCTCCATA CCTAAGTCGA 
GTGCCAAATG ATAAAACGCT GTTGAATCCA CCAGATGGCA GTGGCAACTC ACACTCTAAA 
GGAGGAAGCT CTTGCTCCTC GAATAGCTCT TCAGTCAGCG GATTGCCAGG CGGGGACTTA 
CCCACGGATA ATATAAAGCT GGAGCCAGGT ACAGAACCAC AAGTCCAACC GATGGAGACT 
AACGAAATAA AACAGGAACC CGGAGTGGGA AAAGGAGGCA CTGCAGCAGG TGAAACCAAA 
CCAACTCTGA TCCTCGGAGA TCCGTTGGGA ATGGGCGAGA CTTTGGAGGA CATCAACCCG 
TCAGCCATTG TCCTTTATGT GGTCAATCCG TTCACTTTCG CCTCGGATAG CTGCGAGCTG 
GAGCGTCTAG CTCTGATTGC TCTACTTCGT TGCTATGCGG AACTGCTGAA AGCAGTCCCA 
GATTCAGTGC GATCCCAGAT GAACATACAG ATTATATCGC TAGAATCGGT AATGGAACTG 
GGTCCGTGCG GCAATCGAAA GCGTTTCTCG GACGAGATTA GGTGCCTGGC TCTAAACATA 
TTTTCGCAGT GCCGGCGACA TCTGGTGCAT GCCCAATCCG TTAAAAGTCT TACTGGCTTT 
GGTACGGCGG CTAATATGGA GGCGTTTCTC AAGACAAAGG ATGAACCCAA TCGCCGGGCC 
TACAAGATGT ACACGGCTCC CTTTGTTCTG GCACCAATGC ACGAGAGGAA TGACAAAACG 
GACTTCTCCA GATCAGCGGG AAGTATGCAT GGCCAGAATG AACATCGTTA TTCGGTTATG 
TATTGCAATT ACTGCCTAAG TGAGGATCAG GCTTGGCTTT TGGCCACCGC CACTGATGAA 
AGGGGCGAGA TGCTGGAGAA GATCTGCATT AATATTGATG TGCCAAATCG TGCTCGGAGG 
AGAAAAGCCC CTGCTCGCTA TGTGGCCCTA AAGAAACTGA TGGACTTCAT CATGGGAATC 
ATCTCGCAGA CATCGCAAAT GTGGCGTTTG GTTATCGGAC GCATTGGAAG GATCGGACAC 
AGTGAACTGA AGTCGTGGAG TTTTTTGCTC AGCAAGCAAC AGCTGCAAAA GGCATCCAAG 
CAATTTAAGG ATATGTGTAA GCAATGTACA TTGATGTATC CACCCACGAT CCTGAGTGCT 
TGCCTTGTGA CCTTGGAGCC GGATGCCAAG CTGCGCGTGA TGCCCGACCA GTTTACGCCA 
GATGAGCGCT TCTCACAGAT TTCCATGCAG AACCCGTTGG CCACTCCACA GGACGTGACC 
TGCACCCACA TCCTGGTCTT TCCCACTAGC GCCGTCTGTG CGCCTTTTAC GCGCCAGTTC 
CAAAACGAGC CGCAAGTAGA TGACGACTTT CTAACATTCG AGGAAGAGGG CAACGAGGAC 
TTCAGCGATG CGGACATTGG AGATCTCTTC TGGGACACTC ATATGGACAG AGTATCCAAT 
CATGGTAGTC CCGGTCGCAT GGATGACAAT CGGAGTTGGC AGAGCGCTGG CGGTAATAAT 
TTCAAGTGCA CGCCGCCCCA GGAAGTAGAG GAGGTTGGTT CACTCAACCA GCAGCCCATA 
TCAGTGGGTT ACATGGTGTC CACGGCGCCA ACCGGTCGCA TGCCTGCTTG GTTCTGGTCC 
GCCTGTCCGC ATCTAGAAGA CGTTTGTCCG GTGTTTCTGA AGACAGCCCT CCATCTCCAT 
GTGCCCAGCA TACAGTCTGC CGACGATATT CTCAACTCGA CCAATGCCCA TCAGTCAGGC 
AACGATCATC CGCTTGACTC CAATCTCACA GCGGATGTGC TGCGCTTCGT CCTCGAGGGA 
TACAATGCCC TTTCCTGGCT GGCGCTCGAC TCCAACACAC ACGATCGTCT CTCCTGTCTG 
CCCATCAATG TCCAGACGCT AATGGATCTG TACTATCTGA CGGCTGCAAT AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

skd ( NM_001275205.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262134.1 Drosophila melanogaster skuld (skd), transcript variant F, mRNA.

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CloneID	OFa21255
Clone ID Related Accession (Same CDS sequence)	NM_001275205.1
Accession Version	NM_001275205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	skuld, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCATC AAAATCATCA GACAAACGGC GCAAGTTTGG AGGATTGCCA CACAAACTTT 
TATGCCTTGA CTGATTTATG TGGAATAAAG TGGCGGAAGT TCGTTAACGG GGAGCGACCG 
AACGCATCGA GCGATCCTTT GGCGGACCCG ATCCTGCGCT CATATTCGCG ATGCATCCAG 
GCGGACATGC TGTGCGTGTG GAGGAGGGTC CAATCCACGA AGACGGACCA CGCCGACCCG 
AATGCCTTAA CCTTCGAGAT GACCACGTCG ACCAAGGTCC ACCCGCCCCT TTCGCTGGCC 
GCCGCCAAGG AGCTATGGAT CTTCTGGTAC GGCGAGGAGC CCGATCTCAG TGAACTGGTC 
GATGCCGAGC TGCTCCGAGT AGCGGCCAAC CAAGCGCTTT GGAATGGCAC CTGGAAAGGA 
GCTCTCACCT ACGAGTGCCG GTCGCTGCTC TTTAAGGCGC TGCACAATCT CATGGAGAGA 
TTCGTGCTTA CCAAGGACAT TGTGCGCTTT GGAAAATGGT TTGTGCAGCC CTGCACCTCC 
AGCGATCGCC TCTTTGGACG CAGCTCCCAG CACTTGTCCT TCTCATTTAC GTTCTTTGTG 
CACGGCGACA CCGTTTGTGC CTCCATAGAT TTGCGCGAGC ATCCCGCCGT GCGTCCGCTG 
ACCAAGGAGC ACTTGACCGA AGCGGCGGCA GCCTTTGCAG CGGCCAGTTC TCCGCCAGGA 
TCAAATGGAT CTGCGGCGTC AGCAGGGGGA GCTGTGCCTA ATCCTGGTCA GGATCCAAAT 
GGAGCCAGCA TGGATGGACT GGACGGCGGA GAGGGAGCGG CCAAGGCAGC GCCACCTCCG 
CACGCGCGAA AGGTTATGCT GGCGCCCTTT GGAATTGCTG GCATACTTAC CGGCAATAGC 
TATAAGGCCA GCGATCCCAT AGCCGAGAAG ATCCTTGAGG ATTGGGCCTC GTTTTTTCCG 
CTGTGCAATA AGGATAACAC GGATGTGCCA CCCGTGGTGG AAGTGGTGTC GGGTGGTCAT 
AAGATGTATC ATCCCACGAA CTACGTACTA GTCACAGACC TGGACGACAT GGAGCACATG 
GAATTCGTCG AGATGCAGAA GATGCAAAGC TCAGTGGCCG GAGCGGCGGC CGAGTCAGCT 
GTTCTGGCCT CGCTTTCCTG CCCACCGGGA GCAGCATCTG CTCCTTCATC GATGGGAGCA 
GCTCCATCTG CAACAGCGGT TCCCTTGGGT CCAGCTTCCC TCAATGCGCC AGCTTTAGCA 
GGAGCAGGTG TCGGAGCCAC CGCATCTTCC GCCGCCAAAG AGATATCCCG CAAGGCAGCT 
CCACAAGCGG TTAGCGCCCT GGAACGTCTC GCCTTCCAGC CCTACTACGA TCAACGGCCC 
ACCTCGGGCT TCACCTTCAA TACCAACAAT ACTCACATAC CCGCCTCGGC AGCCGTGGAA 
ATGCCGGAAC GAACCTGGCA GGATTGCGTG ATGAACACGC TCCATGTGGA TGCAGCGGCG 
GCGGCAGCAG CAGTTGCTTC GTCTACGCCC GCCTCGGGAA CTGGGTCATT ATCCGCGGAT 
GGGGACGAGA ATGAGCAGAA TAAACCGCCA CAGGACTCCA AGCAACTGGT GCAGCAGCAG 
ATCCAGCAGC AACAGCAACG CCAAAAGCTC TGGAACTTTG TGGATCCCAT GCAAAAGGCA 
CCCTGCATAT GCACCAAACA TCTGGGAAAC ACACCTCATG GAACACCACA CGGCGGCGCT 
TCAACGTACT CCCGCAACTC CTTGGGCGGC GATTCCTCGA TGCCGGTGGC CTCCGTGGAA 
TCACCGGCAA CGCCGGCTCC CTCTCCGCAT CCGAATTCGG CGCACTCGCA ACCGACATCC 
GTGCCGCCCG CTGAGCAACC GCCGACGCCT ATCGACCACC TGCTGGACAA GAATACACCG 
GCGCCAACGC CAACGGACCA GCACGACAGC AAGAGCATTA CGGCCTCGCC CTATGTTCAT 
CAGACGCCAA GCGTGGAGCC GCCCTCGTAT ACGGATCATG CGGCCGGCGG AGGGCCAGCT 
GGTGGGCAGG GCCTTGGTAC GGGTCCAGGT AGTGTGCCCG CCCAGCAACC GGCTACGCCC 
ACAGCAGCAA CGTCAGCTGG TGGCGCCGGA TCTGGCGGAC CTTCAAATGC AATTGGAGCA 
AATGCGGTTG GTACTATAAG TGTCAAGAAG CTTGAGATGC AGCAGCAGAC TCCATCGGCA 
GCTATGGCCA TCAAGCAGGA GCCTGGTGCT CAAGGTCGGG GGGTTGGAGG TGTTACCTCC 
ACCACGGAGG CCTTGAACAA CTTTAAGCGA CTGTACAACC CGCCAAAGCT GACGTTAAAG 
GATCCGGACA GCTTCTACGA CGAGGAGTGG CTCAAGGAGG TCATCTATGA CTTTCAGTAC 
CAGGAATACT GGGACTATAG CACGGTGAAA CGTCCGAAGA TGGAAAAGCA ACGGAGACCT 
AGGTATGCCA AGAACCTTTA CGAAGGACAA AACCACGTGA AGCCCGTGAT GCCTTCGCCA 
GGATCCGTTT ATGGCTCACA GTTGCTTTCC TTGGATGAGT CAGCGAGCCA AGCAGGAGGG 
AGAGGCGGTG GAGGACAGGC GGCGGGCAGC TCCGGCGGTG GTCTAGTGGG CATTGCCAAC 
AGTACTGCCA GTGGCAGTGA TGTAGAAGCT GATGGTAGCA GCTTCTTCCA GGGACTGAAC 
ATTAAGACAG AGCCTGGATT GCATTCCCCT TCTTGCAAGG AAACGTCGAA ATCCTCGGGC 
GGCAACAGCA GCGGTGGAGG AAGTGGTAGC GGTGGTAATC TCTTCACAGC TGAAGGCTTG 
AATCCCTCGC TCAACGATCT GGAGCAGTTG TTTGAGACAA GCTCGAACGA CGAGTGCAGC 
AGTGTGCAAA TCCACACGCC ACCCGATTCT AACAACCCCT CGAATGGCGG CTGCAGTGCT 
GTGACCAATA CGATCGAAGA CCTCAAACGG AGCACAGCGG TGGCCAGTGC TGCCGTAGCA 
GCGGCAGCAG CAGCTGCGGC CTCGGGCGCG GGCAATATCC AGGCGGAGGA TCTTACCAAG 
ATGTTTCCCA CACCACCGTC TCACGAGCAG CAGCACCCGA ACTCGAGTCC CTGTCAAACG 
GACGTGGTTA TGACGGATCT CAGTGTGGAC ACCACCACAA CCATTATAAC CAGTAGTATC 
ACCACTACTT GCAATACCAC TATCACGAGT AGTATCAACA CCACCACAAC AGCCTGTAGC 
AACCCAAGCA ACAGTATTAT GCTGGCTGCC GCACAAGCTC CCGTCACCGT GGTGGCCATC 
CAGACTGTCT CCAAAATGGT AAAGCAGGAG TATAACCTGG AGCTGGGTAG TCCTATGGAA 
GAGCCCATTA ATGACTGGGA TTATGTGTAC CGACCTCCGC AACAGGAGAA GTTTGTGGGC 
TCGACGCGAT ATGCTCCATT GACCAACCTG CCCAGTCAGA CACAGCCGCC GCTGACTCTG 
CCTACGGGAT GCTTTTATCA ACCCACCTGG AGTAGTCACA AATCAAGAGC AGCAACTTTG 
GCTAAAGCAG CGGCAGCTCA ACAGCAGCAG CATCAGAAAC ATCAGGCCCT TCAGCAGCGC 
ATCCAATTGC ATCAGCAGAA ACTACAGCAG CTCCAGCTTC AGAATCAGCA GCAACAACAA 
GCGGCAGCCG CAGCAGCAGC GGCGGCTAGT GGAGGAGTTG GCCACCAGAA GCACCAGCAT 
CAGCACCTTC ATGATCTCCT GTCAGCGGCG CCGAGGACAC CATTAACGCC CTCTACTGTT 
CCGCAACCGT TGAGCAGTGG TGGCAGTCAG TATTTGCTGA ATCAGTTGAA CTGCCCACAA 
GCGCCACCTG GTGCCTCCAT GCAGCAGCTA ATGCACCGAG CGGGGATGTC ACCAATTTCA 
CCGGGACCCG GAATGGGTCC GTATGCCGCC AGAAGTAGTC CAATGTCGAG GGCAACCCCC 
ACGCATCCGC CACCGCCATA TCCGTATGAC TTGGCTGTGG CCAGTCCAGC TACCTCCACA 
TCATCGTACT TGAACCGGCC GCTTCACTCG CAAGAGCATC CGCACATGCA TGGCTTGGGC 
GGCGGAGCAA CGGGAGTTGT GGGTCACGGA ACAGGAGGTG GCGGGCACAT GGGTATGGTA 
GCGTACACTG GCGATGCGGG CATTGTCAGT GGTGGAACAG CGATGGCAGC CGGATCCTCG 
TCCTTGCTTC AGGAATTACC AGAAGTAAAT TCTGTCCTAG TTAACATCCT GCTGTACGAT 
ACCGCCTTGA ATGTCTTCCG GGATCACAAC TTTGATAGTA GCAGTGTGTG TGTGTGCAAT 
GCGGATACGC AAAAGATTGG CAATATTCGC GGAGCGGATT CCGGAGTATA TGTACCCCTG 
CCCGGGGTTA GCTTCAATCC TTTCCCTTCC GGAGCTGGAG GCGCTTTGGC TGGACAGCGA 
ATGCTCAATG GACCCAGTTC CGCCGGTTTT GGCGGCATGC GGATGATCAG TGCCTTTGGG 
GGTTCGCCAG CCTCAGCCTC GATGCCCGGA GCTGGCTCCG GACATGGCCA TGGTCCAAAT 
GGTGGCTCCA ACTCGTCGTC TTGCACGCCG CCCAGCAGTA ATCCCCATAT CACCGGGTAT 
GTGGACGATG ATCCCGTGGA GTGTACGTGT GGTTTCAGTG CAGTGGTTAA TCGAAGACTC 
TCTCATCGCG CTGGACTCTT TTACGAAGAT GAGGTGGAGA TCACGGGCAT CGCGGATGAT 
CCGGGCAGGA ATAAGCAGCC CACGTTGCTC AGTATCATCC AGAGTCTTAG CAGGAAAAAC 
CAAAACAAGC AGGGACCTGG AGAAACAAGC TCTGCATTGG ATAAAATTGG AGCGGGAGGA 
TTGCCTAATG GACAACTGGA GCAATTGGGT CATGCTGTAT TTGACCTGCT ATTGGATCAG 
TGCTCTATCA TCCAAACATC TAGCAGTTCG GTGCACAGAG CACTTCAGTC TCATCGGAGG 
CGAATGTCCC GCCAACGAAG GATCTTCGGT AACAATGGAG CTCCCACTGC CTCATTGGCA 
TCAATTGCAA ATGTTCTAGA GTTTATGGAT GCACACGACG TTATAAGTTT GGCCCTGGAG 
CAGTCGAGAT TGGCATTCGA AAACCAGCGG ATGGACAACA TGATGGACTT CCATGGGAAT 
GGTAGCAGTA GCTCGCATCA GCAGCAGCAA CTGACAGCTT TCCATGCGCC ACCACCTGCT 
TTGCGTCACA AGCTAGCAGG TATTGGTGCT GGGCGGTTGA CGGTTCACAA GTGGCCATAT 
CTTCCCGTCG GTTTCACCCG TAGCAATAAG GAGATTGTAC GCACCATGAA TGCTATACAG 
CCGATGCTGC AGAATGCATT CCATTGCAAA TCCAGGGGCG GCTCAGGTTC CAAGGATGCC 
AGTTCGTACA ATACGGTGAG TGGACCGCTA ACCTGGCGCC AATTCCATCG ACTGGCAGGC 
CGTGCATCCG GACAATGTGA GCCGCAACCG ATACCATCCG TGGTGGTGGG CTACGAGAAG 
GATTGGATCT CGGTGGCACC GCACTCCATC CACTATTGGG ATAAGTTTCT CCTGGAACCG 
TACTCCTATG CCAGGGATGT TGTATACGTG GTAGTGTGTC CGGACAACGA GCATGTGGTA 
AATTGTACTC GCAGCTATTT CCGGGAACTA AGCAGCACCT ACGAGATGTG CAAGCTGGGC 
AAACACACAC CCATCCGAGG ATGGGATGGT TTCCTTCAAG TAGGCGCTGC TCGCAACAAC 
GTGCCTGCGG ATCGGGAAAC AACTCCCTTG GATGATTGGC TGCGTACCCT GGAACATGCT 
GCTCTGGCGG AGCAAATCCG TCGGTATGCA GTTGCCTTTA TTCACCAGTT GGCTCCATAC 
CTAAGTCGAG TGCCAAATGA TAAAACGCTG TTGAATCCAC CAGATGGCAG TGGCAACTCA 
CACTCTAAAG GAGGAAGCTC TTGCTCCTCG AATAGCTCTT CAGTCAGCGG ATTGCCAGGC 
GGGGACTTAC CCACGGATAA TATAAAGCTG GAGCCAGGTA CAGAACCACA AGTCCAACCG 
ATGGAGACTA ACGAAATAAA ACAGGAACCC GGAGTGGGAA AAGGAGGCAC TGCAGCAGGT 
GAAACCAAAC CAACTCTGAT CCTCGGAGAT CCGTTGGGAA TGGGCGAGAC TTTGGAGGAC 
ATCAACCCGT CAGCCATTGT CCTTTATGTG GTCAATCCGT TCACTTTCGC CTCGGATAGC 
TGCGAGCTGG AGCGTCTAGC TCTGATTGCT CTACTTCGTT GCTATGCGGA ACTGCTGAAA 
GCAGTCCCAG ATTCAGTGCG ATCCCAGATG AACATACAGA TTATATCGCT AGAATCGGTA 
ATGGAACTGG GTCCGTGCGG CAATCGAAAG CGTTTCTCGG ACGAGATTAG GTGCCTGGCT 
CTAAACATAT TTTCGCAGTG CCGGCGACAT CTGGTGCATG CCCAATCCGT TAAAAGTCTT 
ACTGGCTTTG GTACGGCGGC TAATATGGAG GCGTTTCTCA AGACAAAGGA TGAACCCAAT 
CGCCGGGCCT ACAAGATGTA CACGGCTCCC TTTGTTCTGG CACCAATGCA CGAGAGGAAT 
GACAAAACGG ACTTCTCCAG ATCAGCGGGA AGTATGCATG GCCAGAATGA ACATCGTTAT 
TCGGTTATGT ATTGCAATTA CTGCCTAAGT GAGGATCAGG CTTGGCTTTT GGCCACCGCC 
ACTGATGAAA GGGGCGAGAT GCTGGAGAAG ATCTGCATTA ATATTGATGT GCCAAATCGT 
GCTCGGAGGA GAAAAGCCCC TGCTCGCTAT GTGGCCCTAA AGAAACTGAT GGACTTCATC 
ATGGGAATCA TCTCGCAGAC ATCGCAAATG TGGCGTTTGG TTATCGGACG CATTGGAAGG 
ATCGGACACA GTGAACTGAA GTCGTGGAGT TTTTTGCTCA GCAAGCAACA GCTGCAAAAG 
GCATCCAAGC AATTTAAGGA TATGTGTAAG CAATGTACAT TGATGTATCC ACCCACGATC 
CTGAGTGCTT GCCTTGTGAC CTTGGAGCCG GATGCCAAGC TGCGCGTGAT GCCCGACCAG 
TTTACGCCAG ATGAGCGCTT CTCACAGATT TCCATGCAGA ACCCGTTGGC CACTCCACAG 
GACGTGACCT GCACCCACAT CCTGGTCTTT CCCACTAGCG CCGTCTGTGC GCCTTTTACG 
CGCCAGTTCC AAAACGAGCC GCAAGTAGAT GACGACTTTC TAACATTCGA GGAAGAGGGC 
AACGAGGACT TCAGCGATGC GGACATTGGA GATCTCTTCT GGGACACTCA TATGGACAGA 
GTATCCAATC ATGGTAGTCC CGGTCGCATG GATGACAATC GGAGTTGGCA GAGCGCTGGC 
GGTAATAATT TCAAGTGCAC GCCGCCCCAG GAAGTAGAGG AGGTTGGTTC ACTCAACCAG 
CAGCCCATAT CAGTGGGTTA CATGGTGTCC ACGGCGCCAA CCGGTCGCAT GCCTGCTTGG 
TTCTGGTCCG CCTGTCCGCA TCTAGAAGAC GTTTGTCCGG TGTTTCTGAA GACAGCCCTC 
CATCTCCATG TGCCCAGCAT ACAGTCTGCC GACGATATTC TCAACTCGAC CAATGCCCAT 
CAGTCAGGCA ACGATCATCC GCTTGACTCC AATCTCACAG CGGATGTGCT GCGCTTCGTC 
CTCGAGGGAT ACAATGCCCT TTCCTGGCTG GCGCTCGACT CCAACACACA CGATCGTCTC 
TCCTGTCTGC CCATCAATGT CCAGACGCTA ATGGATCTGT ACTATCTGAC GGCTGCAATA 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262134.1
CDS	604..8409
Translation	MTHQNHQTNGASLEDCHTNFYALTDLCGIKWRKFVNGERPNASSDPLADPILRSYSRCIQADMLCVWRRVQSTKTDHADPNALTFEMTTSTKVHPPLSLAAAKELWIFWYGEEPDLSELVDAELLRVAANQALWNGTWKGALTYECRSLLFKALHNLMERFVLTKDIVRFGKWFVQPCTSSDRLFGRSSQHLSFSFTFFVHGDTVCASIDLREHPAVRPLTKEHLTEAAAAFAAASSPPGSNGSAASAGGAVPNPGQDPNGASMDGLDGGEGAAKAAPPPHARKVMLAPFGIAGILTGNSYKASDPIAEKILEDWASFFPLCNKDNTDVPPVVEVVSGGHKMYHPTNYVLVTDLDDMEHMEFVEMQKMQSSVAGAAAESAVLASLSCPPGAASAPSSMGAAPSATAVPLGPASLNAPALAGAGVGATASSAAKEISRKAAPQAVSALERLAFQPYYDQRPTSGFTFNTNNTHIPASAAVEMPERTWQDCVMNTLHVDAAAAAAAVASSTPASGTGSLSADGDENEQNKPPQDSKQLVQQQIQQQQQRQKLWNFVDPMQKAPCICTKHLGNTPHGTPHGGASTYSRNSLGGDSSMPVASVESPATPAPSPHPNSAHSQPTSVPPAEQPPTPIDHLLDKNTPAPTPTDQHDSKSITASPYVHQTPSVEPPSYTDHAAGGGPAGGQGLGTGPGSVPAQQPATPTAATSAGGAGSGGPSNAIGANAVGTISVKKLEMQQQTPSAAMAIKQEPGAQGRGVGGVTSTTEALNNFKRLYNPPKLTLKDPDSFYDEEWLKEVIYDFQYQEYWDYSTVKRPKMEKQRRPRYAKNLYEGQNHVKPVMPSPGSVYGSQLLSLDESASQAGGRGGGGQAAGSSGGGLVGIANSTASGSDVEADGSSFFQGLNIKTEPGLHSPSCKETSKSSGGNSSGGGSGSGGNLFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSAVTNTIEDLKRSTAVASAAVAAAAAAAASGAGNIQAEDLTKMFPTPPSHEQQHPNSSPCQTDVVMTDLSVDTTTTIITSSITTTCNTTITSSINTTTTACSNPSNSIMLAAAQAPVTVVAIQTVSKMVKQEYNLELGSPMEEPINDWDYVYRPPQQEKFVGSTRYAPLTNLPSQTQPPLTLPTGCFYQPTWSSHKSRAATLAKAAAAQQQQHQKHQALQQRIQLHQQKLQQLQLQNQQQQQAAAAAAAAASGGVGHQKHQHQHLHDLLSAAPRTPLTPSTVPQPLSSGGSQYLLNQLNCPQAPPGASMQQLMHRAGMSPISPGPGMGPYAARSSPMSRATPTHPPPPYPYDLAVASPATSTSSYLNRPLHSQEHPHMHGLGGGATGVVGHGTGGGGHMGMVAYTGDAGIVSGGTAMAAGSSSLLQELPEVNSVLVNILLYDTALNVFRDHNFDSSSVCVCNADTQKIGNIRGADSGVYVPLPGVSFNPFPSGAGGALAGQRMLNGPSSAGFGGMRMISAFGGSPASASMPGAGSGHGHGPNGGSNSSSCTPPSSNPHITGYVDDDPVECTCGFSAVVNRRLSHRAGLFYEDEVEITGIADDPGRNKQPTLLSIIQSLSRKNQNKQGPGETSSALDKIGAGGLPNGQLEQLGHAVFDLLLDQCSIIQTSSSSVHRALQSHRRRMSRQRRIFGNNGAPTASLASIANVLEFMDAHDVISLALEQSRLAFENQRMDNMMDFHGNGSSSSHQQQQLTAFHAPPPALRHKLAGIGAGRLTVHKWPYLPVGFTRSNKEIVRTMNAIQPMLQNAFHCKSRGGSGSKDASSYNTVSGPLTWRQFHRLAGRASGQCEPQPIPSVVVGYEKDWISVAPHSIHYWDKFLLEPYSYARDVVYVVVCPDNEHVVNCTRSYFRELSSTYEMCKLGKHTPIRGWDGFLQVGAARNNVPADRETTPLDDWLRTLEHAALAEQIRRYAVAFIHQLAPYLSRVPNDKTLLNPPDGSGNSHSKGGSSCSSNSSSVSGLPGGDLPTDNIKLEPGTEPQVQPMETNEIKQEPGVGKGGTAAGETKPTLILGDPLGMGETLEDINPSAIVLYVVNPFTFASDSCELERLALIALLRCYAELLKAVPDSVRSQMNIQIISLESVMELGPCGNRKRFSDEIRCLALNIFSQCRRHLVHAQSVKSLTGFGTAANMEAFLKTKDEPNRRAYKMYTAPFVLAPMHERNDKTDFSRSAGSMHGQNEHRYSVMYCNYCLSEDQAWLLATATDERGEMLEKICINIDVPNRARRRKAPARYVALKKLMDFIMGIISQTSQMWRLVIGRIGRIGHSELKSWSFLLSKQQLQKASKQFKDMCKQCTLMYPPTILSACLVTLEPDAKLRVMPDQFTPDERFSQISMQNPLATPQDVTCTHILVFPTSAVCAPFTRQFQNEPQVDDDFLTFEEEGNEDFSDADIGDLFWDTHMDRVSNHGSPGRMDDNRSWQSAGGNNFKCTPPQEVEEVGSLNQQPISVGYMVSTAPTGRMPAWFWSACPHLEDVCPVFLKTALHLHVPSIQSADDILNSTNAHQSGNDHPLDSNLTADVLRFVLEGYNALSWLALDSNTHDRLSCLPINVQTLMDLYYLTAAIA

Target ORF information:

RefSeq Version	NM_001275205.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster skuld (skd), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275205.1

ORF Insert Sequence:

ATGACGCATC AAAATCATCA GACAAACGGC GCAAGTTTGG AGGATTGCCA CACAAACTTT 
TATGCCTTGA CTGATTTATG TGGAATAAAG TGGCGGAAGT TCGTTAACGG GGAGCGACCG 
AACGCATCGA GCGATCCTTT GGCGGACCCG ATCCTGCGCT CATATTCGCG ATGCATCCAG 
GCGGACATGC TGTGCGTGTG GAGGAGGGTC CAATCCACGA AGACGGACCA CGCCGACCCG 
AATGCCTTAA CCTTCGAGAT GACCACGTCG ACCAAGGTCC ACCCGCCCCT TTCGCTGGCC 
GCCGCCAAGG AGCTATGGAT CTTCTGGTAC GGCGAGGAGC CCGATCTCAG TGAACTGGTC 
GATGCCGAGC TGCTCCGAGT AGCGGCCAAC CAAGCGCTTT GGAATGGCAC CTGGAAAGGA 
GCTCTCACCT ACGAGTGCCG GTCGCTGCTC TTTAAGGCGC TGCACAATCT CATGGAGAGA 
TTCGTGCTTA CCAAGGACAT TGTGCGCTTT GGAAAATGGT TTGTGCAGCC CTGCACCTCC 
AGCGATCGCC TCTTTGGACG CAGCTCCCAG CACTTGTCCT TCTCATTTAC GTTCTTTGTG 
CACGGCGACA CCGTTTGTGC CTCCATAGAT TTGCGCGAGC ATCCCGCCGT GCGTCCGCTG 
ACCAAGGAGC ACTTGACCGA AGCGGCGGCA GCCTTTGCAG CGGCCAGTTC TCCGCCAGGA 
TCAAATGGAT CTGCGGCGTC AGCAGGGGGA GCTGTGCCTA ATCCTGGTCA GGATCCAAAT 
GGAGCCAGCA TGGATGGACT GGACGGCGGA GAGGGAGCGG CCAAGGCAGC GCCACCTCCG 
CACGCGCGAA AGGTTATGCT GGCGCCCTTT GGAATTGCTG GCATACTTAC CGGCAATAGC 
TATAAGGCCA GCGATCCCAT AGCCGAGAAG ATCCTTGAGG ATTGGGCCTC GTTTTTTCCG 
CTGTGCAATA AGGATAACAC GGATGTGCCA CCCGTGGTGG AAGTGGTGTC GGGTGGTCAT 
AAGATGTATC ATCCCACGAA CTACGTACTA GTCACAGACC TGGACGACAT GGAGCACATG 
GAATTCGTCG AGATGCAGAA GATGCAAAGC TCAGTGGCCG GAGCGGCGGC CGAGTCAGCT 
GTTCTGGCCT CGCTTTCCTG CCCACCGGGA GCAGCATCTG CTCCTTCATC GATGGGAGCA 
GCTCCATCTG CAACAGCGGT TCCCTTGGGT CCAGCTTCCC TCAATGCGCC AGCTTTAGCA 
GGAGCAGGTG TCGGAGCCAC CGCATCTTCC GCCGCCAAAG AGATATCCCG CAAGGCAGCT 
CCACAAGCGG TTAGCGCCCT GGAACGTCTC GCCTTCCAGC CCTACTACGA TCAACGGCCC 
ACCTCGGGCT TCACCTTCAA TACCAACAAT ACTCACATAC CCGCCTCGGC AGCCGTGGAA 
ATGCCGGAAC GAACCTGGCA GGATTGCGTG ATGAACACGC TCCATGTGGA TGCAGCGGCG 
GCGGCAGCAG CAGTTGCTTC GTCTACGCCC GCCTCGGGAA CTGGGTCATT ATCCGCGGAT 
GGGGACGAGA ATGAGCAGAA TAAACCGCCA CAGGACTCCA AGCAACTGGT GCAGCAGCAG 
ATCCAGCAGC AACAGCAACG CCAAAAGCTC TGGAACTTTG TGGATCCCAT GCAAAAGGCA 
CCCTGCATAT GCACCAAACA TCTGGGAAAC ACACCTCATG GAACACCACA CGGCGGCGCT 
TCAACGTACT CCCGCAACTC CTTGGGCGGC GATTCCTCGA TGCCGGTGGC CTCCGTGGAA 
TCACCGGCAA CGCCGGCTCC CTCTCCGCAT CCGAATTCGG CGCACTCGCA ACCGACATCC 
GTGCCGCCCG CTGAGCAACC GCCGACGCCT ATCGACCACC TGCTGGACAA GAATACACCG 
GCGCCAACGC CAACGGACCA GCACGACAGC AAGAGCATTA CGGCCTCGCC CTATGTTCAT 
CAGACGCCAA GCGTGGAGCC GCCCTCGTAT ACGGATCATG CGGCCGGCGG AGGGCCAGCT 
GGTGGGCAGG GCCTTGGTAC GGGTCCAGGT AGTGTGCCCG CCCAGCAACC GGCTACGCCC 
ACAGCAGCAA CGTCAGCTGG TGGCGCCGGA TCTGGCGGAC CTTCAAATGC AATTGGAGCA 
AATGCGGTTG GTACTATAAG TGTCAAGAAG CTTGAGATGC AGCAGCAGAC TCCATCGGCA 
GCTATGGCCA TCAAGCAGGA GCCTGGTGCT CAAGGTCGGG GGGTTGGAGG TGTTACCTCC 
ACCACGGAGG CCTTGAACAA CTTTAAGCGA CTGTACAACC CGCCAAAGCT GACGTTAAAG 
GATCCGGACA GCTTCTACGA CGAGGAGTGG CTCAAGGAGG TCATCTATGA CTTTCAGTAC 
CAGGAATACT GGGACTATAG CACGGTGAAA CGTCCGAAGA TGGAAAAGCA ACGGAGACCT 
AGGTATGCCA AGAACCTTTA CGAAGGACAA AACCACGTGA AGCCCGTGAT GCCTTCGCCA 
GGATCCGTTT ATGGCTCACA GTTGCTTTCC TTGGATGAGT CAGCGAGCCA AGCAGGAGGG 
AGAGGCGGTG GAGGACAGGC GGCGGGCAGC TCCGGCGGTG GTCTAGTGGG CATTGCCAAC 
AGTACTGCCA GTGGCAGTGA TGTAGAAGCT GATGGTAGCA GCTTCTTCCA GGGACTGAAC 
ATTAAGACAG AGCCTGGATT GCATTCCCCT TCTTGCAAGG AAACGTCGAA ATCCTCGGGC 
GGCAACAGCA GCGGTGGAGG AAGTGGTAGC GGTGGTAATC TCTTCACAGC TGAAGGCTTG 
AATCCCTCGC TCAACGATCT GGAGCAGTTG TTTGAGACAA GCTCGAACGA CGAGTGCAGC 
AGTGTGCAAA TCCACACGCC ACCCGATTCT AACAACCCCT CGAATGGCGG CTGCAGTGCT 
GTGACCAATA CGATCGAAGA CCTCAAACGG AGCACAGCGG TGGCCAGTGC TGCCGTAGCA 
GCGGCAGCAG CAGCTGCGGC CTCGGGCGCG GGCAATATCC AGGCGGAGGA TCTTACCAAG 
ATGTTTCCCA CACCACCGTC TCACGAGCAG CAGCACCCGA ACTCGAGTCC CTGTCAAACG 
GACGTGGTTA TGACGGATCT CAGTGTGGAC ACCACCACAA CCATTATAAC CAGTAGTATC 
ACCACTACTT GCAATACCAC TATCACGAGT AGTATCAACA CCACCACAAC AGCCTGTAGC 
AACCCAAGCA ACAGTATTAT GCTGGCTGCC GCACAAGCTC CCGTCACCGT GGTGGCCATC 
CAGACTGTCT CCAAAATGGT AAAGCAGGAG TATAACCTGG AGCTGGGTAG TCCTATGGAA 
GAGCCCATTA ATGACTGGGA TTATGTGTAC CGACCTCCGC AACAGGAGAA GTTTGTGGGC 
TCGACGCGAT ATGCTCCATT GACCAACCTG CCCAGTCAGA CACAGCCGCC GCTGACTCTG 
CCTACGGGAT GCTTTTATCA ACCCACCTGG AGTAGTCACA AATCAAGAGC AGCAACTTTG 
GCTAAAGCAG CGGCAGCTCA ACAGCAGCAG CATCAGAAAC ATCAGGCCCT TCAGCAGCGC 
ATCCAATTGC ATCAGCAGAA ACTACAGCAG CTCCAGCTTC AGAATCAGCA GCAACAACAA 
GCGGCAGCCG CAGCAGCAGC GGCGGCTAGT GGAGGAGTTG GCCACCAGAA GCACCAGCAT 
CAGCACCTTC ATGATCTCCT GTCAGCGGCG CCGAGGACAC CATTAACGCC CTCTACTGTT 
CCGCAACCGT TGAGCAGTGG TGGCAGTCAG TATTTGCTGA ATCAGTTGAA CTGCCCACAA 
GCGCCACCTG GTGCCTCCAT GCAGCAGCTA ATGCACCGAG CGGGGATGTC ACCAATTTCA 
CCGGGACCCG GAATGGGTCC GTATGCCGCC AGAAGTAGTC CAATGTCGAG GGCAACCCCC 
ACGCATCCGC CACCGCCATA TCCGTATGAC TTGGCTGTGG CCAGTCCAGC TACCTCCACA 
TCATCGTACT TGAACCGGCC GCTTCACTCG CAAGAGCATC CGCACATGCA TGGCTTGGGC 
GGCGGAGCAA CGGGAGTTGT GGGTCACGGA ACAGGAGGTG GCGGGCACAT GGGTATGGTA 
GCGTACACTG GCGATGCGGG CATTGTCAGT GGTGGAACAG CGATGGCAGC CGGATCCTCG 
TCCTTGCTTC AGGAATTACC AGAAGTAAAT TCTGTCCTAG TTAACATCCT GCTGTACGAT 
ACCGCCTTGA ATGTCTTCCG GGATCACAAC TTTGATAGTA GCAGTGTGTG TGTGTGCAAT 
GCGGATACGC AAAAGATTGG CAATATTCGC GGAGCGGATT CCGGAGTATA TGTACCCCTG 
CCCGGGGTTA GCTTCAATCC TTTCCCTTCC GGAGCTGGAG GCGCTTTGGC TGGACAGCGA 
ATGCTCAATG GACCCAGTTC CGCCGGTTTT GGCGGCATGC GGATGATCAG TGCCTTTGGG 
GGTTCGCCAG CCTCAGCCTC GATGCCCGGA GCTGGCTCCG GACATGGCCA TGGTCCAAAT 
GGTGGCTCCA ACTCGTCGTC TTGCACGCCG CCCAGCAGTA ATCCCCATAT CACCGGGTAT 
GTGGACGATG ATCCCGTGGA GTGTACGTGT GGTTTCAGTG CAGTGGTTAA TCGAAGACTC 
TCTCATCGCG CTGGACTCTT TTACGAAGAT GAGGTGGAGA TCACGGGCAT CGCGGATGAT 
CCGGGCAGGA ATAAGCAGCC CACGTTGCTC AGTATCATCC AGAGTCTTAG CAGGAAAAAC 
CAAAACAAGC AGGGACCTGG AGAAACAAGC TCTGCATTGG ATAAAATTGG AGCGGGAGGA 
TTGCCTAATG GACAACTGGA GCAATTGGGT CATGCTGTAT TTGACCTGCT ATTGGATCAG 
TGCTCTATCA TCCAAACATC TAGCAGTTCG GTGCACAGAG CACTTCAGTC TCATCGGAGG 
CGAATGTCCC GCCAACGAAG GATCTTCGGT AACAATGGAG CTCCCACTGC CTCATTGGCA 
TCAATTGCAA ATGTTCTAGA GTTTATGGAT GCACACGACG TTATAAGTTT GGCCCTGGAG 
CAGTCGAGAT TGGCATTCGA AAACCAGCGG ATGGACAACA TGATGGACTT CCATGGGAAT 
GGTAGCAGTA GCTCGCATCA GCAGCAGCAA CTGACAGCTT TCCATGCGCC ACCACCTGCT 
TTGCGTCACA AGCTAGCAGG TATTGGTGCT GGGCGGTTGA CGGTTCACAA GTGGCCATAT 
CTTCCCGTCG GTTTCACCCG TAGCAATAAG GAGATTGTAC GCACCATGAA TGCTATACAG 
CCGATGCTGC AGAATGCATT CCATTGCAAA TCCAGGGGCG GCTCAGGTTC CAAGGATGCC 
AGTTCGTACA ATACGGTGAG TGGACCGCTA ACCTGGCGCC AATTCCATCG ACTGGCAGGC 
CGTGCATCCG GACAATGTGA GCCGCAACCG ATACCATCCG TGGTGGTGGG CTACGAGAAG 
GATTGGATCT CGGTGGCACC GCACTCCATC CACTATTGGG ATAAGTTTCT CCTGGAACCG 
TACTCCTATG CCAGGGATGT TGTATACGTG GTAGTGTGTC CGGACAACGA GCATGTGGTA 
AATTGTACTC GCAGCTATTT CCGGGAACTA AGCAGCACCT ACGAGATGTG CAAGCTGGGC 
AAACACACAC CCATCCGAGG ATGGGATGGT TTCCTTCAAG TAGGCGCTGC TCGCAACAAC 
GTGCCTGCGG ATCGGGAAAC AACTCCCTTG GATGATTGGC TGCGTACCCT GGAACATGCT 
GCTCTGGCGG AGCAAATCCG TCGGTATGCA GTTGCCTTTA TTCACCAGTT GGCTCCATAC 
CTAAGTCGAG TGCCAAATGA TAAAACGCTG TTGAATCCAC CAGATGGCAG TGGCAACTCA 
CACTCTAAAG GAGGAAGCTC TTGCTCCTCG AATAGCTCTT CAGTCAGCGG ATTGCCAGGC 
GGGGACTTAC CCACGGATAA TATAAAGCTG GAGCCAGGTA CAGAACCACA AGTCCAACCG 
ATGGAGACTA ACGAAATAAA ACAGGAACCC GGAGTGGGAA AAGGAGGCAC TGCAGCAGGT 
GAAACCAAAC CAACTCTGAT CCTCGGAGAT CCGTTGGGAA TGGGCGAGAC TTTGGAGGAC 
ATCAACCCGT CAGCCATTGT CCTTTATGTG GTCAATCCGT TCACTTTCGC CTCGGATAGC 
TGCGAGCTGG AGCGTCTAGC TCTGATTGCT CTACTTCGTT GCTATGCGGA ACTGCTGAAA 
GCAGTCCCAG ATTCAGTGCG ATCCCAGATG AACATACAGA TTATATCGCT AGAATCGGTA 
ATGGAACTGG GTCCGTGCGG CAATCGAAAG CGTTTCTCGG ACGAGATTAG GTGCCTGGCT 
CTAAACATAT TTTCGCAGTG CCGGCGACAT CTGGTGCATG CCCAATCCGT TAAAAGTCTT 
ACTGGCTTTG GTACGGCGGC TAATATGGAG GCGTTTCTCA AGACAAAGGA TGAACCCAAT 
CGCCGGGCCT ACAAGATGTA CACGGCTCCC TTTGTTCTGG CACCAATGCA CGAGAGGAAT 
GACAAAACGG ACTTCTCCAG ATCAGCGGGA AGTATGCATG GCCAGAATGA ACATCGTTAT 
TCGGTTATGT ATTGCAATTA CTGCCTAAGT GAGGATCAGG CTTGGCTTTT GGCCACCGCC 
ACTGATGAAA GGGGCGAGAT GCTGGAGAAG ATCTGCATTA ATATTGATGT GCCAAATCGT 
GCTCGGAGGA GAAAAGCCCC TGCTCGCTAT GTGGCCCTAA AGAAACTGAT GGACTTCATC 
ATGGGAATCA TCTCGCAGAC ATCGCAAATG TGGCGTTTGG TTATCGGACG CATTGGAAGG 
ATCGGACACA GTGAACTGAA GTCGTGGAGT TTTTTGCTCA GCAAGCAACA GCTGCAAAAG 
GCATCCAAGC AATTTAAGGA TATGTGTAAG CAATGTACAT TGATGTATCC ACCCACGATC 
CTGAGTGCTT GCCTTGTGAC CTTGGAGCCG GATGCCAAGC TGCGCGTGAT GCCCGACCAG 
TTTACGCCAG ATGAGCGCTT CTCACAGATT TCCATGCAGA ACCCGTTGGC CACTCCACAG 
GACGTGACCT GCACCCACAT CCTGGTCTTT CCCACTAGCG CCGTCTGTGC GCCTTTTACG 
CGCCAGTTCC AAAACGAGCC GCAAGTAGAT GACGACTTTC TAACATTCGA GGAAGAGGGC 
AACGAGGACT TCAGCGATGC GGACATTGGA GATCTCTTCT GGGACACTCA TATGGACAGA 
GTATCCAATC ATGGTAGTCC CGGTCGCATG GATGACAATC GGAGTTGGCA GAGCGCTGGC 
GGTAATAATT TCAAGTGCAC GCCGCCCCAG GAAGTAGAGG AGGTTGGTTC ACTCAACCAG 
CAGCCCATAT CAGTGGGTTA CATGGTGTCC ACGGCGCCAA CCGGTCGCAT GCCTGCTTGG 
TTCTGGTCCG CCTGTCCGCA TCTAGAAGAC GTTTGTCCGG TGTTTCTGAA GACAGCCCTC 
CATCTCCATG TGCCCAGCAT ACAGTCTGCC GACGATATTC TCAACTCGAC CAATGCCCAT 
CAGTCAGGCA ACGATCATCC GCTTGACTCC AATCTCACAG CGGATGTGCT GCGCTTCGTC 
CTCGAGGGAT ACAATGCCCT TTCCTGGCTG GCGCTCGACT CCAACACACA CGATCGTCTC 
TCCTGTCTGC CCATCAATGT CCAGACGCTA ATGGATCTGT ACTATCTGAC GGCTGCAATA 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

skd ( NM_168880.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730591.2 Drosophila melanogaster skuld (skd), transcript variant E, mRNA.

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CloneID	OFa19334
Clone ID Related Accession (Same CDS sequence)	NM_168880.3
Accession Version	NM_168880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	skuld, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168880.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCATC AAAATCATCA GACAAACGGC GCAAGTTTGG AGGATTGCCA CACAAACTTT 
TATGCCTTGA CTGATTTATG TGGAATAAAG TGGCGGAAGT TCGTTAACGG GGAGCGACCG 
AACGCATCGA GCGATCCTTT GGCGGACCCG ATCCTGCGCT CATATTCGCG ATGCATCCAG 
GCGGACATGC TGTGCGTGTG GAGGAGGGTC CAATCCACGA AGACGGACCA CGCCGACCCG 
AATGCCTTAA CCTTCGAGAT GACCACGTCG ACCAAGGTCC ACCCGCCCCT TTCGCTGGCC 
GCCGCCAAGG AGCTATGGAT CTTCTGGTAC GGCGAGGAGC CCGATCTCAG TGAACTGGTC 
GATGCCGAGC TGCTCCGAGT AGCGGCCAAC CAAGCGCTTT GGAATGGCAC CTGGAAAGGA 
GCTCTCACCT ACGAGTGCCG GTCGCTGCTC TTTAAGGCGC TGCACAATCT CATGGAGAGA 
TTCGTGCTTA CCAAGGACAT TGTGCGCTTT GGAAAATGGT TTGTGCAGCC CTGCACCTCC 
AGCGATCGCC TCTTTGGACG CAGCTCCCAG CACTTGTCCT TCTCATTTAC GTTCTTTGTG 
CACGGCGACA CCGTTTGTGC CTCCATAGAT TTGCGCGAGC ATCCCGCCGT GCGTCCGCTG 
ACCAAGGAGC ACTTGACCGA AGCGGCGGCA GCCTTTGCAG CGGCCAGTTC TCCGCCAGGA 
TCAAATGGAT CTGCGGCGTC AGCAGGGGGA GCTGTGCCTA ATCCTGGTCA GGATCCAAAT 
GGAGCCAGCA TGGATGGACT GGACGGCGGA GAGGGAGCGG CCAAGGCAGC GCCACCTCCG 
CACGCGCGAA AGGTTATGCT GGCGCCCTTT GGAATTGCTG GCATACTTAC CGGCAATAGC 
TATAAGGCCA GCGATCCCAT AGCCGAGAAG ATCCTTGAGG ATTGGGCCTC GTTTTTTCCG 
CTGTGCAATA AGGATAACAC GGATGTGCCA CCCGTGGTGG AAGTGGTGTC GGGTGGTCAT 
AAGATGTATC ATCCCACGAA CTACGTACTA GTCACAGACC TGGACGACAT GGAGCACATG 
GAATTCGTCG AGATGCAGAA GATGCAAAGC TCAGTGGCCG GAGCGGCGGC CGAGTCAGCT 
GTTCTGGCCT CGCTTTCCTG CCCACCGGGA GCAGCATCTG CTCCTTCATC GATGGGAGCA 
GCTCCATCTG CAACAGCGGT TCCCTTGGGT CCAGCTTCCC TCAATGCGCC AGCTTTAGCA 
GGAGCAGGTG TCGGAGCCAC CGCATCTTCC GCCGCCAAAG AGATATCCCG CAAGGCAGCT 
CCACAAGCGG TTAGCGCCCT GGAACGTCTC GCCTTCCAGC CCTACTACGA TCAACGGCCC 
ACCTCGGGCT TCACCTTCAA TACCAACAAT ACTCACATAC CCGCCTCGGC AGCCGTGGAA 
ATGCCGGAAC GAACCTGGCA GGATTGCGTG ATGAACACGC TCCATGTGGA TGCAGCGGCG 
GCGGCAGCAG CAGTTGCTTC GTCTACGCCC GCCTCGGGAA CTGGGTCATT ATCCGCGGAT 
GGGGACGAGA ATGAGCAGAA TAAACCGCCA CAGGACTCCA AGCAACTGGT GCAGCAGCAG 
ATCCAGCAGC AACAGCAACG CCAAAAGCTC TGGAACTTTG TGGATCCCAT GCAAAAGGCA 
CCCTGCATAT GCACCAACGC CCAGTGCAAC AGCAGTAGCA GCATTAGCAA CATCAGCAAC 
AACAAGCGAC CGCAGCAACC GCTGGGATCG CCTGCGCTGC GAGCAGCAGT TACCGGTGGC 
AGTGGCAATA AGTCGTCCTC GTCATCATCT TCATCATCGT CGTCGTCGTC GTCGTCGTCG 
TCCTACCAGC AGCAGCATTA CCACCAGCAA CATCTGCAGC AACAGCAGCA GCAGCGACCG 
GGAACACCTG TGCTGCCGGC AACGGCGGCC ACATCATCCC ATCATTCCAG TTCCCTGAGC 
AACACTAGCG CCTCCAATTC TTCCGCGGCA ACTCCCCGGC GTCACAATGT GCCGTTCCAT 
AAGCGGTTGC TCCATTCGCT GGCATCCACC GCCTCCTCCA CGGCCTCCAC CTTTAGAGCC 
AAAAACCACA GCAACACCAC CACAGACACC AATATACCAA AACAAAGACA TCTGGGAAAC 
ACACCTCATG GAACACCACA CGGCGGCGCT TCAACGTACT CCCGCAACTC CTTGGGCGGC 
GATTCCTCGA TGCCGGTGGC CTCCGTGGAA TCACCGGCAA CGCCGGCTCC CTCTCCGCAT 
CCGAATTCGG CGCACTCGCA ACCGACATCC GTGCCGCCCG CTGAGCAACT GCTCAATATG 
AGTCCTCATG CGCCAACTTC CGTTTCCAAT CTCCAACAGC CGCCGACGCC TATCGACCAC 
CTGCTGGACA AGAATACACC GGCGCCAACG CCAACGGACC AGCACGACAG CAAGAGCATT 
ACGGCCTCGC CCTATGTTCA TCAGACGCCA AGCGTGGAGC CGCCCTCGTA TACGGATCAT 
GCGGCCGGCG GAGGGCCAGC TGGTGGGCAG GGCCTTGGTA CGGGTCCAGG TAGTGTGCCC 
GCCCAGCAAC CGGCTACGCC CACAGCAGCA ACGTCAGCTG GTGGCGCCGG ATCTGGCGGA 
CCTTCAAATG CAATTGGAGC AAATGCGGTT GGTACTATAA GTGTCAAGAA GCTTGAGATG 
CAGCAGCAGA CTCCATCGGC AGCTATGGCC ATCAAGCAGG AGCCTGGTGC TCAAGGTCGG 
GGGGTTGGAG GTGTTACCTC CACCACGGAG GCCTTGAACA ACTTTAAGCG ACTGTACAAC 
CCGCCAAAGC TGACGTTAAA GGATCCGGAC AGCTTCTACG ACGAGGAGTG GCTCAAGGAG 
GTCATCTATG ACTTTCAGTA CCAGGAATAC TGGGACTATA GCACGGTGAA ACGTCCGAAG 
ATGGAAAAGC AACGGAGACC TAGGTATGCC AAGAACCTTT ACGAAGGACA AAACCACGTG 
AAGCCCGTGA TGCCTTCGCC AGGATCCGTT TATGGCTCAC AGTTGCTTTC CTTGGATGAG 
TCAGCGAGCC AAGCAGGAGG GAGAGGCGGT GGAGGACAGG CGGCGGGCAG CTCCGGCGGT 
GGTCTAGTGG GCATTGCCAA CAGTACTGCC AGTGGCAGTG ATGTAGAAGC TGATGGTAGC 
AGCTTCTTCC AGGGACTGAA CATTAAGACA GAGCCTGGAT TGCATTCCCC TTCTTGCAAG 
GAAACGTCGA AATCCTCGGG CGGCAACAGC AGCGGTGGAG GAAGTGGTAG CGGTGGTAAT 
CTCTTCACAG CTGAAGGCTT GAATCCCTCG CTCAACGATC TGGAGCAGTT GTTTGAGACA 
AGCTCGAACG ACGAGTGCAG CAGTGTGCAA ATCCACACGC CACCCGATTC TAACAACCCC 
TCGAATGGCG GCTGCAGTGC TGTGACCAAT ACGATCGAAG ACCTCAAACG GAGCACAGCG 
GTGGCCAGTG CTGCCGTAGC AGCGGCAGCA GCAGCTGCGG CCTCGGGCGC GGGCAATATC 
CAGGCGGAGG ATCTTACCAA GATGTTTCCC ACACCACCGT CTCACGAGCA GCAGCACCCG 
AACTCGAGTC CCTGTCAAAC GGACGTGGTT ATGACGGATC TCAGTGTGGA CACCACCACA 
ACCATTATAA CCAGTAGTAT CACCACTACT TGCAATACCA CTATCACGAG TAGTATCAAC 
ACCACCACAA CAGCCTGTAG CAACCCAAGC AACAGTATTA TGCTGGCTGC CGCACAAGCT 
CCCGTCACCG TGGTGGCCAT CCAGACTGTC TCCAAAATGG TAAAGCAGGA GTATAACCTG 
GAGCTGGGTA GTCCTATGGA AGAGCCCATT AATGACTGGG ATTATGTGTA CCGACCTCCG 
CAACAGGAGA AGTTTGTGGG CTCGACGCGA TATGCTCCAT TGACCAACCT GCCCAGTCAG 
ACACAGCCGC CGCTGACTCT GCCTACGGGA TGCTTTTATC AACCCACCTG GAGTAGTCAC 
AAATCAAGAG CAGCAACTTT GGCTAAAGCA GCGGCAGCTC AACAGCAGCA GCATCAGAAA 
CATCAGGCCC TTCAGCAGCG CATCCAATTG CATCAGCAGA AACTACAGCA GCTCCAGCTT 
CAGAATCAGC AGCAACAACA AGCGGCAGCC GCAGCAGCAG CGGCGGCTAG TGGAGGAGTT 
GGCCACCAGA AGCACCAGCA TCAGCACCTT CATGATCTCC TGTCAGCGGC GCCGAGGACA 
CCATTAACGC CCTCTACTGT TCCGCAACCG TTGAGCAGTG GTGGCAGTCA GTATTTGCTG 
AATCAGTTGA ACTGCCCACA AGCGCCACCT GGTGCCTCCA TGCAGCAGCT AATGCACCGA 
GCGGGGATGT CACCAATTTC ACCGGGACCC GGAATGGGTC CGTATGCCGC CAGAAGTAGT 
CCAATGTCGA GGGCAACCCC CACGCATCCG CCACCGCCAT ATCCGTATGA CTTGGCTGTG 
GCCAGTCCAG CTACCTCCAC ATCATCGTAC TTGAACCGGC CGCTTCACTC GCAAGAGCAT 
CCGCACATGC ATGGCTTGGG CGGCGGAGCA ACGGGAGTTG TGGGTCACGG AACAGGAGGT 
GGCGGGCACA TGGGTATGGT AGCGTACACT GGCGATGCGG GCATTGTCAG TGGTGGAACA 
GCGATGGCAG CCGGATCCTC GTCCTTGCTT CAGGAATTAC CAGAAGTAAA TTCTGTCCTA 
GTTAACATCC TGCTGTACGA TACCGCCTTG AATGTCTTCC GGGATCACAA CTTTGATAGT 
AGCAGTGTGT GTGTGTGCAA TGCGGATACG CAAAAGATTG GCAATATTCG CGGAGCGGAT 
TCCGGAGTAT ATGTACCCCT GCCCGGGGTT AGCTTCAATC CTTTCCCTTC CGGAGCTGGA 
GGCGCTTTGG CTGGACAGCG AATGCTCAAT GGACCCAGTT CCGCCGGTTT TGGCGGCATG 
CGGATGATCA GTGCCTTTGG GGGTTCGCCA GCCTCAGCCT CGATGCCCGG AGCTGGCTCC 
GGACATGGCC ATGGTCCAAA TGGTGGCTCC AACTCGTCGT CTTGCACGCC GCCCAGCAGT 
AATCCCCATA TCACCGGGTA TGTGGACGAT GATCCCGTGG AGTGTACGTG TGGTTTCAGT 
GCAGTGGTTA ATCGAAGACT CTCTCATCGC GCTGGACTCT TTTACGAAGA TGAGGTGGAG 
ATCACGGGCA TCGCGGATGA TCCGGGCAGG AATAAGCAGC CCACGTTGCT CAGTATCATC 
CAGAGTCTTA GCAGGAAAAA CCAAAACAAG CAGGGACCTG GAGAAACAAG CTCTGCATTG 
GATAAAATTG GAGCGGGAGG ATTGCCTAAT GGACAACTGG AGCAATTGGG TCATGCTGTA 
TTTGACCTGC TATTGGATCA GTGCTCTATC ATCCAAACAT CTAGCAGTTC GGTGCACAGA 
GCACTTCAGT CTCATCGGAG GCGAATGTCC CGCCAACGAA GGATCTTCGG TAACAATGGA 
GCTCCCACTG CCTCATTGGC ATCAATTGCA AATGTTCTAG AGTTTATGGA TGCACACGAC 
GTTATAAGTT TGGCCCTGGA GCAGTCGAGA TTGGCATTCG AAAACCAGCG GATGGACAAC 
ATGATGGACT TCCATGGGAA TGGTAGCAGT AGCTCGCATC AGCAGCAGCA ACTGACAGCT 
TTCCATGCGC CACCACCTGC TTTGCGTCAC AAGCTAGCAG GTATTGGTGC TGGGCGGTTG 
ACGGTTCACA AGTGGCCATA TCTTCCCGTC GGTTTCACCC GTAGCAATAA GGAGATTGTA 
CGCACCATGA ATGCTATACA GCCGATGCTG CAGAATGCAT TCCATTGCAA ATCCAGGGGC 
GGCTCAGGTT CCAAGGATGC CAGTTCGTAC AATACGGTGA GTGGACCGCT AACCTGGCGC 
CAATTCCATC GACTGGCAGG CCGTGCATCC GGACAATGTG AGCCGCAACC GATACCATCC 
GTGGTGGTGG GCTACGAGAA GGATTGGATC TCGGTGGCAC CGCACTCCAT CCACTATTGG 
GATAAGTTTC TCCTGGAACC GTACTCCTAT GCCAGGGATG TTGTATACGT GGTAGTGTGT 
CCGGACAACG AGCATGTGGT AAATTGTACT CGCAGCTATT TCCGGGAACT AAGCAGCACC 
TACGAGATGT GCAAGCTGGG CAAACACACA CCCATCCGAG GATGGGATGG TTTCCTTCAA 
GTAGGCGCTG CTCGCAACAA CGTGCCTGCG GATCGGGAAA CAACTCCCTT GGATGATTGG 
CTGCGTACCC TGGAACATGC TGCTCTGGCG GAGCAAATCC GTCGGTATGC AGTTGCCTTT 
ATTCACCAGT TGGCTCCATA CCTAAGTCGA GTGCCAAATG ATAAAACGCT GTTGAATCCA 
CCAGATGGCA GTGGCAACTC ACACTCTAAA GGAGGAAGCT CTTGCTCCTC GAATAGCTCT 
TCAGTCAGCG GATTGCCAGG CGGGGACTTA CCCACGGATA ATATAAAGCT GGAGCCAGGT 
ACAGAACCAC AAGTCCAACC GATGGAGACT AACGAAATAA AACAGGAACC CGGAGTGGGA 
AAAGGAGGCA CTGCAGCAGG TGAAACCAAA CCAACTCTGA TCCTCGGAGA TCCGTTGGGA 
ATGGGCGAGA CTTTGGAGGA CATCAACCCG TCAGCCATTG TCCTTTATGT GGTCAATCCG 
TTCACTTTCG CCTCGGATAG CTGCGAGCTG GAGCGTCTAG CTCTGATTGC TCTACTTCGT 
TGCTATGCGG AACTGCTGAA AGCAGTCCCA GATTCAGTGC GATCCCAGAT GAACATACAG 
ATTATATCGC TAGAATCGGT AATGGAACTG GGTCCGTGCG GCAATCGAAA GCGTTTCTCG 
GACGAGATTA GGTGCCTGGC TCTAAACATA TTTTCGCAGT GCCGGCGACA TCTGGTGCAT 
GCCCAATCCG TTAAAAGTCT TACTGGCTTT GGTACGGCGG CTAATATGGA GGCGTTTCTC 
AAGACAAAGG ATGAACCCAA TCGCCGGGCC TACAAGATGT ACACGGCTCC CTTTGTTCTG 
GCACCAATGC ACGAGAGGAA TGACAAAACG GACTTCTCCA GATCAGCGGG AAGTATGCAT 
GGCCAGAATG AACATCGTTA TTCGGTTATG TATTGCAATT ACTGCCTAAG TGAGGATCAG 
GCTTGGCTTT TGGCCACCGC CACTGATGAA AGGGGCGAGA TGCTGGAGAA GATCTGCATT 
AATATTGATG TGCCAAATCG TGCTCGGAGG AGAAAAGCCC CTGCTCGCTA TGTGGCCCTA 
AAGAAACTGA TGGACTTCAT CATGGGAATC ATCTCGCAGA CATCGCAAAT GTGGCGTTTG 
GTTATCGGAC GCATTGGAAG GATCGGACAC AGTGAACTGA AGTCGTGGAG TTTTTTGCTC 
AGCAAGCAAC AGCTGCAAAA GGCATCCAAG CAATTTAAGG ATATGTGTAA GCAATGTACA 
TTGATGTATC CACCCACGAT CCTGAGTGCT TGCCTTGTGA CCTTGGAGCC GGATGCCAAG 
CTGCGCGTGA TGCCCGACCA GTTTACGCCA GATGAGCGCT TCTCACAGAT TTCCATGCAG 
AACCCGTTGG CCACTCCACA GGACGTGACC TGCACCCACA TCCTGGTCTT TCCCACTAGC 
GCCGTCTGTG CGCCTTTTAC GCGCCAGTTC CAAAACGAGC CGCAAGTAGA TGACGACTTT 
CTAACATTCG AGGAAGAGGG CAACGAGGAC TTCAGCGATG CGGACATTGG AGATCTCTTC 
TGGGACACTC ATATGGACAG AGTATCCAAT CATGGTAGTC CCGGTCGCAT GGATGACAAT 
CGGAGTTGGC AGAGCGCTGG CGGTAATAAT TTCAAGTGCA CGCCGCCCCA GGAAGTAGAG 
GAGGTTGGTT CACTCAACCA GCAGCCCATA TCAGTGGGTT ACATGGTGTC CACGGCGCCA 
ACCGGTCGCA TGCCTGCTTG GTTCTGGTCC GCCTGTCCGC ATCTAGAAGA CGTTTGTCCG 
GTGTTTCTGA AGACAGCCCT CCATCTCCAT GTGCCCAGCA TACAGTCTGC CGACGATATT 
CTCAACTCGA CCAATGCCCA TCAGTCAGGC AACGATCATC CGCTTGACTC CAATCTCACA 
GCGGATGTGC TGCGCTTCGT CCTCGAGGGA TACAATGCCC TTTCCTGGCT GGCGCTCGAC 
TCCAACACAC ACGATCGTCT CTCCTGTCTG CCCATCAATG TCCAGACGCT AATGGATCTG 
TACTATCTGA CGGCTGCAAT AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730591.2
CDS	824..9130
Translation	MTHQNHQTNGASLEDCHTNFYALTDLCGIKWRKFVNGERPNASSDPLADPILRSYSRCIQADMLCVWRRVQSTKTDHADPNALTFEMTTSTKVHPPLSLAAAKELWIFWYGEEPDLSELVDAELLRVAANQALWNGTWKGALTYECRSLLFKALHNLMERFVLTKDIVRFGKWFVQPCTSSDRLFGRSSQHLSFSFTFFVHGDTVCASIDLREHPAVRPLTKEHLTEAAAAFAAASSPPGSNGSAASAGGAVPNPGQDPNGASMDGLDGGEGAAKAAPPPHARKVMLAPFGIAGILTGNSYKASDPIAEKILEDWASFFPLCNKDNTDVPPVVEVVSGGHKMYHPTNYVLVTDLDDMEHMEFVEMQKMQSSVAGAAAESAVLASLSCPPGAASAPSSMGAAPSATAVPLGPASLNAPALAGAGVGATASSAAKEISRKAAPQAVSALERLAFQPYYDQRPTSGFTFNTNNTHIPASAAVEMPERTWQDCVMNTLHVDAAAAAAAVASSTPASGTGSLSADGDENEQNKPPQDSKQLVQQQIQQQQQRQKLWNFVDPMQKAPCICTNAQCNSSSSISNISNNKRPQQPLGSPALRAAVTGGSGNKSSSSSSSSSSSSSSSSSYQQQHYHQQHLQQQQQQRPGTPVLPATAATSSHHSSSLSNTSASNSSAATPRRHNVPFHKRLLHSLASTASSTASTFRAKNHSNTTTDTNIPKQRHLGNTPHGTPHGGASTYSRNSLGGDSSMPVASVESPATPAPSPHPNSAHSQPTSVPPAEQLLNMSPHAPTSVSNLQQPPTPIDHLLDKNTPAPTPTDQHDSKSITASPYVHQTPSVEPPSYTDHAAGGGPAGGQGLGTGPGSVPAQQPATPTAATSAGGAGSGGPSNAIGANAVGTISVKKLEMQQQTPSAAMAIKQEPGAQGRGVGGVTSTTEALNNFKRLYNPPKLTLKDPDSFYDEEWLKEVIYDFQYQEYWDYSTVKRPKMEKQRRPRYAKNLYEGQNHVKPVMPSPGSVYGSQLLSLDESASQAGGRGGGGQAAGSSGGGLVGIANSTASGSDVEADGSSFFQGLNIKTEPGLHSPSCKETSKSSGGNSSGGGSGSGGNLFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSAVTNTIEDLKRSTAVASAAVAAAAAAAASGAGNIQAEDLTKMFPTPPSHEQQHPNSSPCQTDVVMTDLSVDTTTTIITSSITTTCNTTITSSINTTTTACSNPSNSIMLAAAQAPVTVVAIQTVSKMVKQEYNLELGSPMEEPINDWDYVYRPPQQEKFVGSTRYAPLTNLPSQTQPPLTLPTGCFYQPTWSSHKSRAATLAKAAAAQQQQHQKHQALQQRIQLHQQKLQQLQLQNQQQQQAAAAAAAAASGGVGHQKHQHQHLHDLLSAAPRTPLTPSTVPQPLSSGGSQYLLNQLNCPQAPPGASMQQLMHRAGMSPISPGPGMGPYAARSSPMSRATPTHPPPPYPYDLAVASPATSTSSYLNRPLHSQEHPHMHGLGGGATGVVGHGTGGGGHMGMVAYTGDAGIVSGGTAMAAGSSSLLQELPEVNSVLVNILLYDTALNVFRDHNFDSSSVCVCNADTQKIGNIRGADSGVYVPLPGVSFNPFPSGAGGALAGQRMLNGPSSAGFGGMRMISAFGGSPASASMPGAGSGHGHGPNGGSNSSSCTPPSSNPHITGYVDDDPVECTCGFSAVVNRRLSHRAGLFYEDEVEITGIADDPGRNKQPTLLSIIQSLSRKNQNKQGPGETSSALDKIGAGGLPNGQLEQLGHAVFDLLLDQCSIIQTSSSSVHRALQSHRRRMSRQRRIFGNNGAPTASLASIANVLEFMDAHDVISLALEQSRLAFENQRMDNMMDFHGNGSSSSHQQQQLTAFHAPPPALRHKLAGIGAGRLTVHKWPYLPVGFTRSNKEIVRTMNAIQPMLQNAFHCKSRGGSGSKDASSYNTVSGPLTWRQFHRLAGRASGQCEPQPIPSVVVGYEKDWISVAPHSIHYWDKFLLEPYSYARDVVYVVVCPDNEHVVNCTRSYFRELSSTYEMCKLGKHTPIRGWDGFLQVGAARNNVPADRETTPLDDWLRTLEHAALAEQIRRYAVAFIHQLAPYLSRVPNDKTLLNPPDGSGNSHSKGGSSCSSNSSSVSGLPGGDLPTDNIKLEPGTEPQVQPMETNEIKQEPGVGKGGTAAGETKPTLILGDPLGMGETLEDINPSAIVLYVVNPFTFASDSCELERLALIALLRCYAELLKAVPDSVRSQMNIQIISLESVMELGPCGNRKRFSDEIRCLALNIFSQCRRHLVHAQSVKSLTGFGTAANMEAFLKTKDEPNRRAYKMYTAPFVLAPMHERNDKTDFSRSAGSMHGQNEHRYSVMYCNYCLSEDQAWLLATATDERGEMLEKICINIDVPNRARRRKAPARYVALKKLMDFIMGIISQTSQMWRLVIGRIGRIGHSELKSWSFLLSKQQLQKASKQFKDMCKQCTLMYPPTILSACLVTLEPDAKLRVMPDQFTPDERFSQISMQNPLATPQDVTCTHILVFPTSAVCAPFTRQFQNEPQVDDDFLTFEEEGNEDFSDADIGDLFWDTHMDRVSNHGSPGRMDDNRSWQSAGGNNFKCTPPQEVEEVGSLNQQPISVGYMVSTAPTGRMPAWFWSACPHLEDVCPVFLKTALHLHVPSIQSADDILNSTNAHQSGNDHPLDSNLTADVLRFVLEGYNALSWLALDSNTHDRLSCLPINVQTLMDLYYLTAAIA

Target ORF information:

RefSeq Version	NM_168880.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster skuld (skd), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168880.3

ORF Insert Sequence:

ATGACGCATC AAAATCATCA GACAAACGGC GCAAGTTTGG AGGATTGCCA CACAAACTTT 
TATGCCTTGA CTGATTTATG TGGAATAAAG TGGCGGAAGT TCGTTAACGG GGAGCGACCG 
AACGCATCGA GCGATCCTTT GGCGGACCCG ATCCTGCGCT CATATTCGCG ATGCATCCAG 
GCGGACATGC TGTGCGTGTG GAGGAGGGTC CAATCCACGA AGACGGACCA CGCCGACCCG 
AATGCCTTAA CCTTCGAGAT GACCACGTCG ACCAAGGTCC ACCCGCCCCT TTCGCTGGCC 
GCCGCCAAGG AGCTATGGAT CTTCTGGTAC GGCGAGGAGC CCGATCTCAG TGAACTGGTC 
GATGCCGAGC TGCTCCGAGT AGCGGCCAAC CAAGCGCTTT GGAATGGCAC CTGGAAAGGA 
GCTCTCACCT ACGAGTGCCG GTCGCTGCTC TTTAAGGCGC TGCACAATCT CATGGAGAGA 
TTCGTGCTTA CCAAGGACAT TGTGCGCTTT GGAAAATGGT TTGTGCAGCC CTGCACCTCC 
AGCGATCGCC TCTTTGGACG CAGCTCCCAG CACTTGTCCT TCTCATTTAC GTTCTTTGTG 
CACGGCGACA CCGTTTGTGC CTCCATAGAT TTGCGCGAGC ATCCCGCCGT GCGTCCGCTG 
ACCAAGGAGC ACTTGACCGA AGCGGCGGCA GCCTTTGCAG CGGCCAGTTC TCCGCCAGGA 
TCAAATGGAT CTGCGGCGTC AGCAGGGGGA GCTGTGCCTA ATCCTGGTCA GGATCCAAAT 
GGAGCCAGCA TGGATGGACT GGACGGCGGA GAGGGAGCGG CCAAGGCAGC GCCACCTCCG 
CACGCGCGAA AGGTTATGCT GGCGCCCTTT GGAATTGCTG GCATACTTAC CGGCAATAGC 
TATAAGGCCA GCGATCCCAT AGCCGAGAAG ATCCTTGAGG ATTGGGCCTC GTTTTTTCCG 
CTGTGCAATA AGGATAACAC GGATGTGCCA CCCGTGGTGG AAGTGGTGTC GGGTGGTCAT 
AAGATGTATC ATCCCACGAA CTACGTACTA GTCACAGACC TGGACGACAT GGAGCACATG 
GAATTCGTCG AGATGCAGAA GATGCAAAGC TCAGTGGCCG GAGCGGCGGC CGAGTCAGCT 
GTTCTGGCCT CGCTTTCCTG CCCACCGGGA GCAGCATCTG CTCCTTCATC GATGGGAGCA 
GCTCCATCTG CAACAGCGGT TCCCTTGGGT CCAGCTTCCC TCAATGCGCC AGCTTTAGCA 
GGAGCAGGTG TCGGAGCCAC CGCATCTTCC GCCGCCAAAG AGATATCCCG CAAGGCAGCT 
CCACAAGCGG TTAGCGCCCT GGAACGTCTC GCCTTCCAGC CCTACTACGA TCAACGGCCC 
ACCTCGGGCT TCACCTTCAA TACCAACAAT ACTCACATAC CCGCCTCGGC AGCCGTGGAA 
ATGCCGGAAC GAACCTGGCA GGATTGCGTG ATGAACACGC TCCATGTGGA TGCAGCGGCG 
GCGGCAGCAG CAGTTGCTTC GTCTACGCCC GCCTCGGGAA CTGGGTCATT ATCCGCGGAT 
GGGGACGAGA ATGAGCAGAA TAAACCGCCA CAGGACTCCA AGCAACTGGT GCAGCAGCAG 
ATCCAGCAGC AACAGCAACG CCAAAAGCTC TGGAACTTTG TGGATCCCAT GCAAAAGGCA 
CCCTGCATAT GCACCAACGC CCAGTGCAAC AGCAGTAGCA GCATTAGCAA CATCAGCAAC 
AACAAGCGAC CGCAGCAACC GCTGGGATCG CCTGCGCTGC GAGCAGCAGT TACCGGTGGC 
AGTGGCAATA AGTCGTCCTC GTCATCATCT TCATCATCGT CGTCGTCGTC GTCGTCGTCG 
TCCTACCAGC AGCAGCATTA CCACCAGCAA CATCTGCAGC AACAGCAGCA GCAGCGACCG 
GGAACACCTG TGCTGCCGGC AACGGCGGCC ACATCATCCC ATCATTCCAG TTCCCTGAGC 
AACACTAGCG CCTCCAATTC TTCCGCGGCA ACTCCCCGGC GTCACAATGT GCCGTTCCAT 
AAGCGGTTGC TCCATTCGCT GGCATCCACC GCCTCCTCCA CGGCCTCCAC CTTTAGAGCC 
AAAAACCACA GCAACACCAC CACAGACACC AATATACCAA AACAAAGACA TCTGGGAAAC 
ACACCTCATG GAACACCACA CGGCGGCGCT TCAACGTACT CCCGCAACTC CTTGGGCGGC 
GATTCCTCGA TGCCGGTGGC CTCCGTGGAA TCACCGGCAA CGCCGGCTCC CTCTCCGCAT 
CCGAATTCGG CGCACTCGCA ACCGACATCC GTGCCGCCCG CTGAGCAACT GCTCAATATG 
AGTCCTCATG CGCCAACTTC CGTTTCCAAT CTCCAACAGC CGCCGACGCC TATCGACCAC 
CTGCTGGACA AGAATACACC GGCGCCAACG CCAACGGACC AGCACGACAG CAAGAGCATT 
ACGGCCTCGC CCTATGTTCA TCAGACGCCA AGCGTGGAGC CGCCCTCGTA TACGGATCAT 
GCGGCCGGCG GAGGGCCAGC TGGTGGGCAG GGCCTTGGTA CGGGTCCAGG TAGTGTGCCC 
GCCCAGCAAC CGGCTACGCC CACAGCAGCA ACGTCAGCTG GTGGCGCCGG ATCTGGCGGA 
CCTTCAAATG CAATTGGAGC AAATGCGGTT GGTACTATAA GTGTCAAGAA GCTTGAGATG 
CAGCAGCAGA CTCCATCGGC AGCTATGGCC ATCAAGCAGG AGCCTGGTGC TCAAGGTCGG 
GGGGTTGGAG GTGTTACCTC CACCACGGAG GCCTTGAACA ACTTTAAGCG ACTGTACAAC 
CCGCCAAAGC TGACGTTAAA GGATCCGGAC AGCTTCTACG ACGAGGAGTG GCTCAAGGAG 
GTCATCTATG ACTTTCAGTA CCAGGAATAC TGGGACTATA GCACGGTGAA ACGTCCGAAG 
ATGGAAAAGC AACGGAGACC TAGGTATGCC AAGAACCTTT ACGAAGGACA AAACCACGTG 
AAGCCCGTGA TGCCTTCGCC AGGATCCGTT TATGGCTCAC AGTTGCTTTC CTTGGATGAG 
TCAGCGAGCC AAGCAGGAGG GAGAGGCGGT GGAGGACAGG CGGCGGGCAG CTCCGGCGGT 
GGTCTAGTGG GCATTGCCAA CAGTACTGCC AGTGGCAGTG ATGTAGAAGC TGATGGTAGC 
AGCTTCTTCC AGGGACTGAA CATTAAGACA GAGCCTGGAT TGCATTCCCC TTCTTGCAAG 
GAAACGTCGA AATCCTCGGG CGGCAACAGC AGCGGTGGAG GAAGTGGTAG CGGTGGTAAT 
CTCTTCACAG CTGAAGGCTT GAATCCCTCG CTCAACGATC TGGAGCAGTT GTTTGAGACA 
AGCTCGAACG ACGAGTGCAG CAGTGTGCAA ATCCACACGC CACCCGATTC TAACAACCCC 
TCGAATGGCG GCTGCAGTGC TGTGACCAAT ACGATCGAAG ACCTCAAACG GAGCACAGCG 
GTGGCCAGTG CTGCCGTAGC AGCGGCAGCA GCAGCTGCGG CCTCGGGCGC GGGCAATATC 
CAGGCGGAGG ATCTTACCAA GATGTTTCCC ACACCACCGT CTCACGAGCA GCAGCACCCG 
AACTCGAGTC CCTGTCAAAC GGACGTGGTT ATGACGGATC TCAGTGTGGA CACCACCACA 
ACCATTATAA CCAGTAGTAT CACCACTACT TGCAATACCA CTATCACGAG TAGTATCAAC 
ACCACCACAA CAGCCTGTAG CAACCCAAGC AACAGTATTA TGCTGGCTGC CGCACAAGCT 
CCCGTCACCG TGGTGGCCAT CCAGACTGTC TCCAAAATGG TAAAGCAGGA GTATAACCTG 
GAGCTGGGTA GTCCTATGGA AGAGCCCATT AATGACTGGG ATTATGTGTA CCGACCTCCG 
CAACAGGAGA AGTTTGTGGG CTCGACGCGA TATGCTCCAT TGACCAACCT GCCCAGTCAG 
ACACAGCCGC CGCTGACTCT GCCTACGGGA TGCTTTTATC AACCCACCTG GAGTAGTCAC 
AAATCAAGAG CAGCAACTTT GGCTAAAGCA GCGGCAGCTC AACAGCAGCA GCATCAGAAA 
CATCAGGCCC TTCAGCAGCG CATCCAATTG CATCAGCAGA AACTACAGCA GCTCCAGCTT 
CAGAATCAGC AGCAACAACA AGCGGCAGCC GCAGCAGCAG CGGCGGCTAG TGGAGGAGTT 
GGCCACCAGA AGCACCAGCA TCAGCACCTT CATGATCTCC TGTCAGCGGC GCCGAGGACA 
CCATTAACGC CCTCTACTGT TCCGCAACCG TTGAGCAGTG GTGGCAGTCA GTATTTGCTG 
AATCAGTTGA ACTGCCCACA AGCGCCACCT GGTGCCTCCA TGCAGCAGCT AATGCACCGA 
GCGGGGATGT CACCAATTTC ACCGGGACCC GGAATGGGTC CGTATGCCGC CAGAAGTAGT 
CCAATGTCGA GGGCAACCCC CACGCATCCG CCACCGCCAT ATCCGTATGA CTTGGCTGTG 
GCCAGTCCAG CTACCTCCAC ATCATCGTAC TTGAACCGGC CGCTTCACTC GCAAGAGCAT 
CCGCACATGC ATGGCTTGGG CGGCGGAGCA ACGGGAGTTG TGGGTCACGG AACAGGAGGT 
GGCGGGCACA TGGGTATGGT AGCGTACACT GGCGATGCGG GCATTGTCAG TGGTGGAACA 
GCGATGGCAG CCGGATCCTC GTCCTTGCTT CAGGAATTAC CAGAAGTAAA TTCTGTCCTA 
GTTAACATCC TGCTGTACGA TACCGCCTTG AATGTCTTCC GGGATCACAA CTTTGATAGT 
AGCAGTGTGT GTGTGTGCAA TGCGGATACG CAAAAGATTG GCAATATTCG CGGAGCGGAT 
TCCGGAGTAT ATGTACCCCT GCCCGGGGTT AGCTTCAATC CTTTCCCTTC CGGAGCTGGA 
GGCGCTTTGG CTGGACAGCG AATGCTCAAT GGACCCAGTT CCGCCGGTTT TGGCGGCATG 
CGGATGATCA GTGCCTTTGG GGGTTCGCCA GCCTCAGCCT CGATGCCCGG AGCTGGCTCC 
GGACATGGCC ATGGTCCAAA TGGTGGCTCC AACTCGTCGT CTTGCACGCC GCCCAGCAGT 
AATCCCCATA TCACCGGGTA TGTGGACGAT GATCCCGTGG AGTGTACGTG TGGTTTCAGT 
GCAGTGGTTA ATCGAAGACT CTCTCATCGC GCTGGACTCT TTTACGAAGA TGAGGTGGAG 
ATCACGGGCA TCGCGGATGA TCCGGGCAGG AATAAGCAGC CCACGTTGCT CAGTATCATC 
CAGAGTCTTA GCAGGAAAAA CCAAAACAAG CAGGGACCTG GAGAAACAAG CTCTGCATTG 
GATAAAATTG GAGCGGGAGG ATTGCCTAAT GGACAACTGG AGCAATTGGG TCATGCTGTA 
TTTGACCTGC TATTGGATCA GTGCTCTATC ATCCAAACAT CTAGCAGTTC GGTGCACAGA 
GCACTTCAGT CTCATCGGAG GCGAATGTCC CGCCAACGAA GGATCTTCGG TAACAATGGA 
GCTCCCACTG CCTCATTGGC ATCAATTGCA AATGTTCTAG AGTTTATGGA TGCACACGAC 
GTTATAAGTT TGGCCCTGGA GCAGTCGAGA TTGGCATTCG AAAACCAGCG GATGGACAAC 
ATGATGGACT TCCATGGGAA TGGTAGCAGT AGCTCGCATC AGCAGCAGCA ACTGACAGCT 
TTCCATGCGC CACCACCTGC TTTGCGTCAC AAGCTAGCAG GTATTGGTGC TGGGCGGTTG 
ACGGTTCACA AGTGGCCATA TCTTCCCGTC GGTTTCACCC GTAGCAATAA GGAGATTGTA 
CGCACCATGA ATGCTATACA GCCGATGCTG CAGAATGCAT TCCATTGCAA ATCCAGGGGC 
GGCTCAGGTT CCAAGGATGC CAGTTCGTAC AATACGGTGA GTGGACCGCT AACCTGGCGC 
CAATTCCATC GACTGGCAGG CCGTGCATCC GGACAATGTG AGCCGCAACC GATACCATCC 
GTGGTGGTGG GCTACGAGAA GGATTGGATC TCGGTGGCAC CGCACTCCAT CCACTATTGG 
GATAAGTTTC TCCTGGAACC GTACTCCTAT GCCAGGGATG TTGTATACGT GGTAGTGTGT 
CCGGACAACG AGCATGTGGT AAATTGTACT CGCAGCTATT TCCGGGAACT AAGCAGCACC 
TACGAGATGT GCAAGCTGGG CAAACACACA CCCATCCGAG GATGGGATGG TTTCCTTCAA 
GTAGGCGCTG CTCGCAACAA CGTGCCTGCG GATCGGGAAA CAACTCCCTT GGATGATTGG 
CTGCGTACCC TGGAACATGC TGCTCTGGCG GAGCAAATCC GTCGGTATGC AGTTGCCTTT 
ATTCACCAGT TGGCTCCATA CCTAAGTCGA GTGCCAAATG ATAAAACGCT GTTGAATCCA 
CCAGATGGCA GTGGCAACTC ACACTCTAAA GGAGGAAGCT CTTGCTCCTC GAATAGCTCT 
TCAGTCAGCG GATTGCCAGG CGGGGACTTA CCCACGGATA ATATAAAGCT GGAGCCAGGT 
ACAGAACCAC AAGTCCAACC GATGGAGACT AACGAAATAA AACAGGAACC CGGAGTGGGA 
AAAGGAGGCA CTGCAGCAGG TGAAACCAAA CCAACTCTGA TCCTCGGAGA TCCGTTGGGA 
ATGGGCGAGA CTTTGGAGGA CATCAACCCG TCAGCCATTG TCCTTTATGT GGTCAATCCG 
TTCACTTTCG CCTCGGATAG CTGCGAGCTG GAGCGTCTAG CTCTGATTGC TCTACTTCGT 
TGCTATGCGG AACTGCTGAA AGCAGTCCCA GATTCAGTGC GATCCCAGAT GAACATACAG 
ATTATATCGC TAGAATCGGT AATGGAACTG GGTCCGTGCG GCAATCGAAA GCGTTTCTCG 
GACGAGATTA GGTGCCTGGC TCTAAACATA TTTTCGCAGT GCCGGCGACA TCTGGTGCAT 
GCCCAATCCG TTAAAAGTCT TACTGGCTTT GGTACGGCGG CTAATATGGA GGCGTTTCTC 
AAGACAAAGG ATGAACCCAA TCGCCGGGCC TACAAGATGT ACACGGCTCC CTTTGTTCTG 
GCACCAATGC ACGAGAGGAA TGACAAAACG GACTTCTCCA GATCAGCGGG AAGTATGCAT 
GGCCAGAATG AACATCGTTA TTCGGTTATG TATTGCAATT ACTGCCTAAG TGAGGATCAG 
GCTTGGCTTT TGGCCACCGC CACTGATGAA AGGGGCGAGA TGCTGGAGAA GATCTGCATT 
AATATTGATG TGCCAAATCG TGCTCGGAGG AGAAAAGCCC CTGCTCGCTA TGTGGCCCTA 
AAGAAACTGA TGGACTTCAT CATGGGAATC ATCTCGCAGA CATCGCAAAT GTGGCGTTTG 
GTTATCGGAC GCATTGGAAG GATCGGACAC AGTGAACTGA AGTCGTGGAG TTTTTTGCTC 
AGCAAGCAAC AGCTGCAAAA GGCATCCAAG CAATTTAAGG ATATGTGTAA GCAATGTACA 
TTGATGTATC CACCCACGAT CCTGAGTGCT TGCCTTGTGA CCTTGGAGCC GGATGCCAAG 
CTGCGCGTGA TGCCCGACCA GTTTACGCCA GATGAGCGCT TCTCACAGAT TTCCATGCAG 
AACCCGTTGG CCACTCCACA GGACGTGACC TGCACCCACA TCCTGGTCTT TCCCACTAGC 
GCCGTCTGTG CGCCTTTTAC GCGCCAGTTC CAAAACGAGC CGCAAGTAGA TGACGACTTT 
CTAACATTCG AGGAAGAGGG CAACGAGGAC TTCAGCGATG CGGACATTGG AGATCTCTTC 
TGGGACACTC ATATGGACAG AGTATCCAAT CATGGTAGTC CCGGTCGCAT GGATGACAAT 
CGGAGTTGGC AGAGCGCTGG CGGTAATAAT TTCAAGTGCA CGCCGCCCCA GGAAGTAGAG 
GAGGTTGGTT CACTCAACCA GCAGCCCATA TCAGTGGGTT ACATGGTGTC CACGGCGCCA 
ACCGGTCGCA TGCCTGCTTG GTTCTGGTCC GCCTGTCCGC ATCTAGAAGA CGTTTGTCCG 
GTGTTTCTGA AGACAGCCCT CCATCTCCAT GTGCCCAGCA TACAGTCTGC CGACGATATT 
CTCAACTCGA CCAATGCCCA TCAGTCAGGC AACGATCATC CGCTTGACTC CAATCTCACA 
GCGGATGTGC TGCGCTTCGT CCTCGAGGGA TACAATGCCC TTTCCTGGCT GGCGCTCGAC 
TCCAACACAC ACGATCGTCT CTCCTGTCTG CCCATCAATG TCCAGACGCT AATGGATCTG 
TACTATCTGA CGGCTGCAAT AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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skd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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