AMPKalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	AMPKalpha
Entrez Gene ID	43904
Full Name	AMP-activated protein kinase alpha subunit
Synonyms	AK,AMPK,AMPK-alpha,AmpKalpha,CG3051,DmAMPK alpha,Dmel\CG3051,EG:132E8.2,FBgn0023169,Gprk-4,Gprk4,SNF1A,SNF4A-a,ampk,ampkalpha,dAMPK,dAMPKa,dAMPKalpha,snf1A,snf1a
General protein information	Preferred Names AMP-activated protein kinase alpha subunit Names CG3051 gene product from transcript CG3051-RB 5'monophosphate-activated kinase AMP activated kinase AMP-activated kinase AMP-activated protein kinase AMP-dependent kinase AMPK catalytic subunit AMPKalpha-PA AMPKalpha-PB AMPKalpha-PC CG3051-PA CG3051-PB CG3051-PC G protein-coupled receptor kinase 4 SNF1A/AMP-activated protein kinase adenosine monophosphate-activated protein kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2A1-2A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_166880.2	NP_726730.1	AMP-activated protein kinase alpha subunit, isoform B
NM_206604.2	NP_996327.1	AMP-activated protein kinase alpha subunit, isoform C
NM_057965.4	NP_477313.1	AMP-activated protein kinase alpha subunit, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRKAA2	XP_001144587.1
Mus musculus (house mouse)	Prkaa2	NP_835279.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002686	XP_312237.4
Caenorhabditis elegans (roundworm)	aak-2	NP_510710.2
Neurospora crassa	NCU04566	XP_958665.2
	PRKAA2	XP_002801614.1
Bos taurus (cattle)	PRKAA2	NP_001192534.1
Rattus norvegicus (Norway rat)	Prkaa2	NP_076481.1
Danio rerio (zebrafish)	prkaa2	XP_700831.4
Drosophila melanogaster (fruit fly)	SNF1A	NP_477313.1
Saccharomyces cerevisiae (baker's yeast)	SNF1	NP_010765.3
Schizosaccharomyces pombe (fission yeast)	ssp2	NP_588376.1
Kluyveromyces lactis	KLLA0A03806g	XP_451166.1
Oryza sativa (rice)	Os03g0289100	NP_001049790.1
Canis lupus familiaris (dog)	PRKAA2	XP_005620393.1
Gallus gallus (chicken)	PRKAA2	NP_001034694.1
Eremothecium gossypii	AGOS_AEL230W	NP_984631.1
Oryza sativa (rice)	Os08g0484600	NP_001062083.1
Homo sapiens (human)	PRKAA2	NP_006243.2
Magnaporthe oryzae (rice blast fungus)	MGG_00803	XP_003718181.1
Xenopus tropicalis (tropical clawed frog)	prkaa2	NP_001135554.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AMPK can function outside of its canonical nutrient-sensing role in specific developmental contexts.
Title: AMP-activated protein kinase has diet-dependent and -independent roles in Drosophila oogenesis.

The significance of these findings is that AMPK is important in the regulation of glucagon signaling, suggesting that the organization of metabolic networks is highly conserved and that AMPK plays a prominent role in these networks.
Title: Energy-dependent modulation of glucagon-like signaling in Drosophila via the AMP-activated protein kinase.

Loss of AMPK activity exacerbates neuronal loss and associated phenotypes in parkin and LRRK transgenic flies.
Title: AMP kinase activation mitigates dopaminergic dysfunction and mitochondrial abnormalities in Drosophila models of Parkinson's disease.

Data suggest that that an Sgt1/Hsp90-LKB1-AMPK pathway acts redundantly with a microtubule-induced polarity pathway to generate neuroblast cortical polarity, and the absence of neuroblast cortical polarity can produce neuroblast tumors.
Title: Sgt1 acts via an LKB1/AMPK pathway to establish cortical polarity in larval neuroblasts.

AMPK gamma and alpha mutants altered lipid metabolism and showed persistent starvation behaviors.
Title: Altered metabolism and persistent starvation behaviors caused by reduced AMPK function in Drosophila.

The following AMPKalpha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMPKalpha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02292	NM_166880.2 Latest version!	Drosophila melanogaster AMP-activated protein kinase alpha subunit (AMPKalpha), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02292	NM_057965.4 Latest version!	Drosophila melanogaster AMP-activated protein kinase alpha subunit (AMPKalpha), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02292	NM_206604.2 Latest version!	Drosophila melanogaster AMP-activated protein kinase alpha subunit (AMPKalpha), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AMPKalpha ( NM_166880.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726730.1 Drosophila melanogaster AMP-activated protein kinase alpha subunit (AMPKalpha), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02292
Clone ID Related Accession (Same CDS sequence)	NM_166880.2 , NM_206604.2 , NM_057965.4
Accession Version	NM_166880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	AMP-activated protein kinase alpha subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_166880.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCCAGA TGAGGGCTGC GGCCGCTGAG GCGGTGGCCG CCGGCAGCGC CAATGGCCAG 
CCGCTGGTCA AGATTGGGCA CTACCTACTG GGCGCAACCC TGGGAACGGG AACCTTCGGC 
AAGGTGAAGA TCGGCGAGCA CCAGATCACA CGCGTCAAGG TGGCCGTCAA GATACTCAAT 
CGTCAGAAGA TCAAAAGCCT GGACGTCGTG GGCAAGATCC GGCGTGAGAT CCAGAACCTA 
AAGCTCTTCC GCCATCCGCA CATCATCAAG TTGTACCAGG TCATATCGAC GCCGTCGGAC 
ATCTTCATGA TCATGGAGTA CGTGAGCGGC GGGGAGCTGT TCGACTATAT TGTGAAGCAC 
GGCAAGCTGC AGGAGCACCA AGCGCGCCGC TTTTTTCAGC AGATTATCTC TGGCGTGGAC 
TATTGCCACC GGCACATGAT CGTGCATCGA GACCTGAAGC CAGAGAACCT GCTGCTCGAC 
CACAACATGC ACGTGAAGAT CGCCGACTTT GGGCTCTCGA ACATGATGCT GGACGGCGAG 
TTCCTGCGCA CCTCGTGCGG CTCGCCCAAC TACGCGGCGC CCGAGGTGAT TTCCGGCAAG 
CTGTACGCCG GTCCCGAGGT GGACATCTGG TCGTGCGGCG TCATCCTGTA CGCCCTGCTG 
TGCGGGACGC TGCCCTTTGA CGACGAGCAT GTGCCTACGC TGTTTCGCAA GATCAAGTCG 
GGCATTTTCC CCATTCCCGA GTACCTCAAC AAACAAGTGG TCAATCTGGT CTGTCAGATG 
CTGCAAGTGG ACCCGCTCAA GCGGGCCAAC ATCGAGGAAA TCAAGAAGCA CGAGTGGTTC 
CAAAAGGACC TCCCGGCCTA CCTCTTCCCC TCGTCCATCG AGCAGGACTC CAATGTGATC 
GACACCTACG CTGTGGCAGA GGTCTGCACC AAGTTCGGCG TCAAGGAGAC CGAGGTGCAC 
AACTCGCTGC TCAGCGGCGA TCCGCACGAT CAGCTGGCCA TCGCTTACCA TCTGATCATC 
GACAACAAAC GCTTCGCCGA CGACGCGGCC AACCAAATAA ACGAGATCAA CAACTTCTTT 
GTGGCCGGCT CTCCACCACC GCCGCCGCCT CCGCCCGTTC CCCAATCATC GATGGACCAC 
CAAGCCCCTC TGGCCACCGT GACCGTTGGC GGAGGTACCT CAGCCAGCTC GGGCACCGCC 
ACACCAGTGC CACCAGTGGC TGGAGGCACG CCTAGCAGCA CCATACCCAT TCGACCGCAT 
CCGGAGCGGA TTGCGCCGAT GCGGGACCGT CAACTGGCCA TGTCCGTGCA GACATCTGGC 
GGCGGAGCCT TCCCGGAGAA AACCGCACGT GGCGGCACTC CCATCAAGCG CGCCAAGTGG 
CATCTGGGCA TCCGGTCGCA GAGCAAGCCC AACGACATAA TGCTCGAGGT ATACCGAGCG 
ATGAAGGCAC TCAGCTACGA GTGGAAGATC ATCAATCCGT ACCACGTTCG GGTGCGCAGG 
CAGAACGTGA AGACCGGCAA GTTCTCGAAG ATGTCACTGC AGCTGTACCA GGTGGACGCC 
AAGAGCTATC TGCTCGACTT CAAGTCGCTG ACCAACGACG AGGTCGAGCA GGGCGACGAC 
GTCATCATGG AGAGCCTTAC TCCGCCGCCG CTTAGCGTGT CCGGGGTGAT GCCGCTGCAG 
CCGACCGGCC ACCACACCAT GGAGTTTTTC GAAATGTGCG CCGCCCTGAT CATTCAACTG 
GCTCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726730.1
CDS	374..2122
Translation	MPQMRAAAAEAVAAGSANGQPLVKIGHYLLGATLGTGTFGKVKIGEHQITRVKVAVKILNRQKIKSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMIMEYVSGGELFDYIVKHGKLQEHQARRFFQQIISGVDYCHRHMIVHRDLKPENLLLDHNMHVKIADFGLSNMMLDGEFLRTSCGSPNYAAPEVISGKLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFRKIKSGIFPIPEYLNKQVVNLVCQMLQVDPLKRANIEEIKKHEWFQKDLPAYLFPSSIEQDSNVIDTYAVAEVCTKFGVKETEVHNSLLSGDPHDQLAIAYHLIIDNKRFADDAANQINEINNFFVAGSPPPPPPPPVPQSSMDHQAPLATVTVGGGTSASSGTATPVPPVAGGTPSSTIPIRPHPERIAPMRDRQLAMSVQTSGGGAFPEKTARGGTPIKRAKWHLGIRSQSKPNDIMLEVYRAMKALSYEWKIINPYHVRVRRQNVKTGKFSKMSLQLYQVDAKSYLLDFKSLTNDEVEQGDDVIMESLTPPPLSVSGVMPLQPTGHHTMEFFEMCAALIIQLAR

Target ORF information:

RefSeq Version	NM_166880.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster AMP-activated protein kinase alpha subunit (AMPKalpha), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166880.2

ORF Insert Sequence:

ATGCCCCAGA TGAGGGCTGC GGCCGCTGAG GCGGTGGCCG CCGGCAGCGC CAATGGCCAG 
CCGCTGGTCA AGATTGGGCA CTACCTACTG GGCGCAACCC TGGGAACGGG AACCTTCGGC 
AAGGTGAAGA TCGGCGAGCA CCAGATCACA CGCGTCAAGG TGGCCGTCAA GATACTCAAT 
CGTCAGAAGA TCAAAAGCCT GGACGTCGTG GGCAAGATCC GGCGTGAGAT CCAGAACCTA 
AAGCTCTTCC GCCATCCGCA CATCATCAAG TTGTACCAGG TCATATCGAC GCCGTCGGAC 
ATCTTCATGA TCATGGAGTA CGTGAGCGGC GGGGAGCTGT TCGACTATAT TGTGAAGCAC 
GGCAAGCTGC AGGAGCACCA AGCGCGCCGC TTTTTTCAGC AGATTATCTC TGGCGTGGAC 
TATTGCCACC GGCACATGAT CGTGCATCGA GACCTGAAGC CAGAGAACCT GCTGCTCGAC 
CACAACATGC ACGTGAAGAT CGCCGACTTT GGGCTCTCGA ACATGATGCT GGACGGCGAG 
TTCCTGCGCA CCTCGTGCGG CTCGCCCAAC TACGCGGCGC CCGAGGTGAT TTCCGGCAAG 
CTGTACGCCG GTCCCGAGGT GGACATCTGG TCGTGCGGCG TCATCCTGTA CGCCCTGCTG 
TGCGGGACGC TGCCCTTTGA CGACGAGCAT GTGCCTACGC TGTTTCGCAA GATCAAGTCG 
GGCATTTTCC CCATTCCCGA GTACCTCAAC AAACAAGTGG TCAATCTGGT CTGTCAGATG 
CTGCAAGTGG ACCCGCTCAA GCGGGCCAAC ATCGAGGAAA TCAAGAAGCA CGAGTGGTTC 
CAAAAGGACC TCCCGGCCTA CCTCTTCCCC TCGTCCATCG AGCAGGACTC CAATGTGATC 
GACACCTACG CTGTGGCAGA GGTCTGCACC AAGTTCGGCG TCAAGGAGAC CGAGGTGCAC 
AACTCGCTGC TCAGCGGCGA TCCGCACGAT CAGCTGGCCA TCGCTTACCA TCTGATCATC 
GACAACAAAC GCTTCGCCGA CGACGCGGCC AACCAAATAA ACGAGATCAA CAACTTCTTT 
GTGGCCGGCT CTCCACCACC GCCGCCGCCT CCGCCCGTTC CCCAATCATC GATGGACCAC 
CAAGCCCCTC TGGCCACCGT GACCGTTGGC GGAGGTACCT CAGCCAGCTC GGGCACCGCC 
ACACCAGTGC CACCAGTGGC TGGAGGCACG CCTAGCAGCA CCATACCCAT TCGACCGCAT 
CCGGAGCGGA TTGCGCCGAT GCGGGACCGT CAACTGGCCA TGTCCGTGCA GACATCTGGC 
GGCGGAGCCT TCCCGGAGAA AACCGCACGT GGCGGCACTC CCATCAAGCG CGCCAAGTGG 
CATCTGGGCA TCCGGTCGCA GAGCAAGCCC AACGACATAA TGCTCGAGGT ATACCGAGCG 
ATGAAGGCAC TCAGCTACGA GTGGAAGATC ATCAATCCGT ACCACGTTCG GGTGCGCAGG 
CAGAACGTGA AGACCGGCAA GTTCTCGAAG ATGTCACTGC AGCTGTACCA GGTGGACGCC 
AAGAGCTATC TGCTCGACTT CAAGTCGCTG ACCAACGACG AGGTCGAGCA GGGCGACGAC 
GTCATCATGG AGAGCCTTAC TCCGCCGCCG CTTAGCGTGT CCGGGGTGAT GCCGCTGCAG 
CCGACCGGCC ACCACACCAT GGAGTTTTTC GAAATGTGCG CCGCCCTGAT CATTCAACTG 
GCTCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMPKalpha ( NM_206604.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996327.1 Drosophila melanogaster AMP-activated protein kinase alpha subunit (AMPKalpha), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa02292
Clone ID Related Accession (Same CDS sequence)	NM_166880.2 , NM_206604.2 , NM_057965.4
Accession Version	NM_206604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	AMP-activated protein kinase alpha subunit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206604.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCCAGA TGAGGGCTGC GGCCGCTGAG GCGGTGGCCG CCGGCAGCGC CAATGGCCAG 
CCGCTGGTCA AGATTGGGCA CTACCTACTG GGCGCAACCC TGGGAACGGG AACCTTCGGC 
AAGGTGAAGA TCGGCGAGCA CCAGATCACA CGCGTCAAGG TGGCCGTCAA GATACTCAAT 
CGTCAGAAGA TCAAAAGCCT GGACGTCGTG GGCAAGATCC GGCGTGAGAT CCAGAACCTA 
AAGCTCTTCC GCCATCCGCA CATCATCAAG TTGTACCAGG TCATATCGAC GCCGTCGGAC 
ATCTTCATGA TCATGGAGTA CGTGAGCGGC GGGGAGCTGT TCGACTATAT TGTGAAGCAC 
GGCAAGCTGC AGGAGCACCA AGCGCGCCGC TTTTTTCAGC AGATTATCTC TGGCGTGGAC 
TATTGCCACC GGCACATGAT CGTGCATCGA GACCTGAAGC CAGAGAACCT GCTGCTCGAC 
CACAACATGC ACGTGAAGAT CGCCGACTTT GGGCTCTCGA ACATGATGCT GGACGGCGAG 
TTCCTGCGCA CCTCGTGCGG CTCGCCCAAC TACGCGGCGC CCGAGGTGAT TTCCGGCAAG 
CTGTACGCCG GTCCCGAGGT GGACATCTGG TCGTGCGGCG TCATCCTGTA CGCCCTGCTG 
TGCGGGACGC TGCCCTTTGA CGACGAGCAT GTGCCTACGC TGTTTCGCAA GATCAAGTCG 
GGCATTTTCC CCATTCCCGA GTACCTCAAC AAACAAGTGG TCAATCTGGT CTGTCAGATG 
CTGCAAGTGG ACCCGCTCAA GCGGGCCAAC ATCGAGGAAA TCAAGAAGCA CGAGTGGTTC 
CAAAAGGACC TCCCGGCCTA CCTCTTCCCC TCGTCCATCG AGCAGGACTC CAATGTGATC 
GACACCTACG CTGTGGCAGA GGTCTGCACC AAGTTCGGCG TCAAGGAGAC CGAGGTGCAC 
AACTCGCTGC TCAGCGGCGA TCCGCACGAT CAGCTGGCCA TCGCTTACCA TCTGATCATC 
GACAACAAAC GCTTCGCCGA CGACGCGGCC AACCAAATAA ACGAGATCAA CAACTTCTTT 
GTGGCCGGCT CTCCACCACC GCCGCCGCCT CCGCCCGTTC CCCAATCATC GATGGACCAC 
CAAGCCCCTC TGGCCACCGT GACCGTTGGC GGAGGTACCT CAGCCAGCTC GGGCACCGCC 
ACACCAGTGC CACCAGTGGC TGGAGGCACG CCTAGCAGCA CCATACCCAT TCGACCGCAT 
CCGGAGCGGA TTGCGCCGAT GCGGGACCGT CAACTGGCCA TGTCCGTGCA GACATCTGGC 
GGCGGAGCCT TCCCGGAGAA AACCGCACGT GGCGGCACTC CCATCAAGCG CGCCAAGTGG 
CATCTGGGCA TCCGGTCGCA GAGCAAGCCC AACGACATAA TGCTCGAGGT ATACCGAGCG 
ATGAAGGCAC TCAGCTACGA GTGGAAGATC ATCAATCCGT ACCACGTTCG GGTGCGCAGG 
CAGAACGTGA AGACCGGCAA GTTCTCGAAG ATGTCACTGC AGCTGTACCA GGTGGACGCC 
AAGAGCTATC TGCTCGACTT CAAGTCGCTG ACCAACGACG AGGTCGAGCA GGGCGACGAC 
GTCATCATGG AGAGCCTTAC TCCGCCGCCG CTTAGCGTGT CCGGGGTGAT GCCGCTGCAG 
CCGACCGGCC ACCACACCAT GGAGTTTTTC GAAATGTGCG CCGCCCTGAT CATTCAACTG 
GCTCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996327.1
CDS	260..2008
Translation	MPQMRAAAAEAVAAGSANGQPLVKIGHYLLGATLGTGTFGKVKIGEHQITRVKVAVKILNRQKIKSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMIMEYVSGGELFDYIVKHGKLQEHQARRFFQQIISGVDYCHRHMIVHRDLKPENLLLDHNMHVKIADFGLSNMMLDGEFLRTSCGSPNYAAPEVISGKLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFRKIKSGIFPIPEYLNKQVVNLVCQMLQVDPLKRANIEEIKKHEWFQKDLPAYLFPSSIEQDSNVIDTYAVAEVCTKFGVKETEVHNSLLSGDPHDQLAIAYHLIIDNKRFADDAANQINEINNFFVAGSPPPPPPPPVPQSSMDHQAPLATVTVGGGTSASSGTATPVPPVAGGTPSSTIPIRPHPERIAPMRDRQLAMSVQTSGGGAFPEKTARGGTPIKRAKWHLGIRSQSKPNDIMLEVYRAMKALSYEWKIINPYHVRVRRQNVKTGKFSKMSLQLYQVDAKSYLLDFKSLTNDEVEQGDDVIMESLTPPPLSVSGVMPLQPTGHHTMEFFEMCAALIIQLAR

Target ORF information:

RefSeq Version	NM_206604.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster AMP-activated protein kinase alpha subunit (AMPKalpha), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206604.2

ORF Insert Sequence:

ATGCCCCAGA TGAGGGCTGC GGCCGCTGAG GCGGTGGCCG CCGGCAGCGC CAATGGCCAG 
CCGCTGGTCA AGATTGGGCA CTACCTACTG GGCGCAACCC TGGGAACGGG AACCTTCGGC 
AAGGTGAAGA TCGGCGAGCA CCAGATCACA CGCGTCAAGG TGGCCGTCAA GATACTCAAT 
CGTCAGAAGA TCAAAAGCCT GGACGTCGTG GGCAAGATCC GGCGTGAGAT CCAGAACCTA 
AAGCTCTTCC GCCATCCGCA CATCATCAAG TTGTACCAGG TCATATCGAC GCCGTCGGAC 
ATCTTCATGA TCATGGAGTA CGTGAGCGGC GGGGAGCTGT TCGACTATAT TGTGAAGCAC 
GGCAAGCTGC AGGAGCACCA AGCGCGCCGC TTTTTTCAGC AGATTATCTC TGGCGTGGAC 
TATTGCCACC GGCACATGAT CGTGCATCGA GACCTGAAGC CAGAGAACCT GCTGCTCGAC 
CACAACATGC ACGTGAAGAT CGCCGACTTT GGGCTCTCGA ACATGATGCT GGACGGCGAG 
TTCCTGCGCA CCTCGTGCGG CTCGCCCAAC TACGCGGCGC CCGAGGTGAT TTCCGGCAAG 
CTGTACGCCG GTCCCGAGGT GGACATCTGG TCGTGCGGCG TCATCCTGTA CGCCCTGCTG 
TGCGGGACGC TGCCCTTTGA CGACGAGCAT GTGCCTACGC TGTTTCGCAA GATCAAGTCG 
GGCATTTTCC CCATTCCCGA GTACCTCAAC AAACAAGTGG TCAATCTGGT CTGTCAGATG 
CTGCAAGTGG ACCCGCTCAA GCGGGCCAAC ATCGAGGAAA TCAAGAAGCA CGAGTGGTTC 
CAAAAGGACC TCCCGGCCTA CCTCTTCCCC TCGTCCATCG AGCAGGACTC CAATGTGATC 
GACACCTACG CTGTGGCAGA GGTCTGCACC AAGTTCGGCG TCAAGGAGAC CGAGGTGCAC 
AACTCGCTGC TCAGCGGCGA TCCGCACGAT CAGCTGGCCA TCGCTTACCA TCTGATCATC 
GACAACAAAC GCTTCGCCGA CGACGCGGCC AACCAAATAA ACGAGATCAA CAACTTCTTT 
GTGGCCGGCT CTCCACCACC GCCGCCGCCT CCGCCCGTTC CCCAATCATC GATGGACCAC 
CAAGCCCCTC TGGCCACCGT GACCGTTGGC GGAGGTACCT CAGCCAGCTC GGGCACCGCC 
ACACCAGTGC CACCAGTGGC TGGAGGCACG CCTAGCAGCA CCATACCCAT TCGACCGCAT 
CCGGAGCGGA TTGCGCCGAT GCGGGACCGT CAACTGGCCA TGTCCGTGCA GACATCTGGC 
GGCGGAGCCT TCCCGGAGAA AACCGCACGT GGCGGCACTC CCATCAAGCG CGCCAAGTGG 
CATCTGGGCA TCCGGTCGCA GAGCAAGCCC AACGACATAA TGCTCGAGGT ATACCGAGCG 
ATGAAGGCAC TCAGCTACGA GTGGAAGATC ATCAATCCGT ACCACGTTCG GGTGCGCAGG 
CAGAACGTGA AGACCGGCAA GTTCTCGAAG ATGTCACTGC AGCTGTACCA GGTGGACGCC 
AAGAGCTATC TGCTCGACTT CAAGTCGCTG ACCAACGACG AGGTCGAGCA GGGCGACGAC 
GTCATCATGG AGAGCCTTAC TCCGCCGCCG CTTAGCGTGT CCGGGGTGAT GCCGCTGCAG 
CCGACCGGCC ACCACACCAT GGAGTTTTTC GAAATGTGCG CCGCCCTGAT CATTCAACTG 
GCTCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMPKalpha ( NM_057965.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477313.1 Drosophila melanogaster AMP-activated protein kinase alpha subunit (AMPKalpha), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02292
Clone ID Related Accession (Same CDS sequence)	NM_166880.2 , NM_206604.2 , NM_057965.4
Accession Version	NM_057965.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	AMP-activated protein kinase alpha subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057965.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCCAGA TGAGGGCTGC GGCCGCTGAG GCGGTGGCCG CCGGCAGCGC CAATGGCCAG 
CCGCTGGTCA AGATTGGGCA CTACCTACTG GGCGCAACCC TGGGAACGGG AACCTTCGGC 
AAGGTGAAGA TCGGCGAGCA CCAGATCACA CGCGTCAAGG TGGCCGTCAA GATACTCAAT 
CGTCAGAAGA TCAAAAGCCT GGACGTCGTG GGCAAGATCC GGCGTGAGAT CCAGAACCTA 
AAGCTCTTCC GCCATCCGCA CATCATCAAG TTGTACCAGG TCATATCGAC GCCGTCGGAC 
ATCTTCATGA TCATGGAGTA CGTGAGCGGC GGGGAGCTGT TCGACTATAT TGTGAAGCAC 
GGCAAGCTGC AGGAGCACCA AGCGCGCCGC TTTTTTCAGC AGATTATCTC TGGCGTGGAC 
TATTGCCACC GGCACATGAT CGTGCATCGA GACCTGAAGC CAGAGAACCT GCTGCTCGAC 
CACAACATGC ACGTGAAGAT CGCCGACTTT GGGCTCTCGA ACATGATGCT GGACGGCGAG 
TTCCTGCGCA CCTCGTGCGG CTCGCCCAAC TACGCGGCGC CCGAGGTGAT TTCCGGCAAG 
CTGTACGCCG GTCCCGAGGT GGACATCTGG TCGTGCGGCG TCATCCTGTA CGCCCTGCTG 
TGCGGGACGC TGCCCTTTGA CGACGAGCAT GTGCCTACGC TGTTTCGCAA GATCAAGTCG 
GGCATTTTCC CCATTCCCGA GTACCTCAAC AAACAAGTGG TCAATCTGGT CTGTCAGATG 
CTGCAAGTGG ACCCGCTCAA GCGGGCCAAC ATCGAGGAAA TCAAGAAGCA CGAGTGGTTC 
CAAAAGGACC TCCCGGCCTA CCTCTTCCCC TCGTCCATCG AGCAGGACTC CAATGTGATC 
GACACCTACG CTGTGGCAGA GGTCTGCACC AAGTTCGGCG TCAAGGAGAC CGAGGTGCAC 
AACTCGCTGC TCAGCGGCGA TCCGCACGAT CAGCTGGCCA TCGCTTACCA TCTGATCATC 
GACAACAAAC GCTTCGCCGA CGACGCGGCC AACCAAATAA ACGAGATCAA CAACTTCTTT 
GTGGCCGGCT CTCCACCACC GCCGCCGCCT CCGCCCGTTC CCCAATCATC GATGGACCAC 
CAAGCCCCTC TGGCCACCGT GACCGTTGGC GGAGGTACCT CAGCCAGCTC GGGCACCGCC 
ACACCAGTGC CACCAGTGGC TGGAGGCACG CCTAGCAGCA CCATACCCAT TCGACCGCAT 
CCGGAGCGGA TTGCGCCGAT GCGGGACCGT CAACTGGCCA TGTCCGTGCA GACATCTGGC 
GGCGGAGCCT TCCCGGAGAA AACCGCACGT GGCGGCACTC CCATCAAGCG CGCCAAGTGG 
CATCTGGGCA TCCGGTCGCA GAGCAAGCCC AACGACATAA TGCTCGAGGT ATACCGAGCG 
ATGAAGGCAC TCAGCTACGA GTGGAAGATC ATCAATCCGT ACCACGTTCG GGTGCGCAGG 
CAGAACGTGA AGACCGGCAA GTTCTCGAAG ATGTCACTGC AGCTGTACCA GGTGGACGCC 
AAGAGCTATC TGCTCGACTT CAAGTCGCTG ACCAACGACG AGGTCGAGCA GGGCGACGAC 
GTCATCATGG AGAGCCTTAC TCCGCCGCCG CTTAGCGTGT CCGGGGTGAT GCCGCTGCAG 
CCGACCGGCC ACCACACCAT GGAGTTTTTC GAAATGTGCG CCGCCCTGAT CATTCAACTG 
GCTCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477313.1
CDS	268..2016
Translation	MPQMRAAAAEAVAAGSANGQPLVKIGHYLLGATLGTGTFGKVKIGEHQITRVKVAVKILNRQKIKSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMIMEYVSGGELFDYIVKHGKLQEHQARRFFQQIISGVDYCHRHMIVHRDLKPENLLLDHNMHVKIADFGLSNMMLDGEFLRTSCGSPNYAAPEVISGKLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFRKIKSGIFPIPEYLNKQVVNLVCQMLQVDPLKRANIEEIKKHEWFQKDLPAYLFPSSIEQDSNVIDTYAVAEVCTKFGVKETEVHNSLLSGDPHDQLAIAYHLIIDNKRFADDAANQINEINNFFVAGSPPPPPPPPVPQSSMDHQAPLATVTVGGGTSASSGTATPVPPVAGGTPSSTIPIRPHPERIAPMRDRQLAMSVQTSGGGAFPEKTARGGTPIKRAKWHLGIRSQSKPNDIMLEVYRAMKALSYEWKIINPYHVRVRRQNVKTGKFSKMSLQLYQVDAKSYLLDFKSLTNDEVEQGDDVIMESLTPPPLSVSGVMPLQPTGHHTMEFFEMCAALIIQLAR

Target ORF information:

RefSeq Version	NM_057965.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster AMP-activated protein kinase alpha subunit (AMPKalpha), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057965.4

ORF Insert Sequence:

ATGCCCCAGA TGAGGGCTGC GGCCGCTGAG GCGGTGGCCG CCGGCAGCGC CAATGGCCAG 
CCGCTGGTCA AGATTGGGCA CTACCTACTG GGCGCAACCC TGGGAACGGG AACCTTCGGC 
AAGGTGAAGA TCGGCGAGCA CCAGATCACA CGCGTCAAGG TGGCCGTCAA GATACTCAAT 
CGTCAGAAGA TCAAAAGCCT GGACGTCGTG GGCAAGATCC GGCGTGAGAT CCAGAACCTA 
AAGCTCTTCC GCCATCCGCA CATCATCAAG TTGTACCAGG TCATATCGAC GCCGTCGGAC 
ATCTTCATGA TCATGGAGTA CGTGAGCGGC GGGGAGCTGT TCGACTATAT TGTGAAGCAC 
GGCAAGCTGC AGGAGCACCA AGCGCGCCGC TTTTTTCAGC AGATTATCTC TGGCGTGGAC 
TATTGCCACC GGCACATGAT CGTGCATCGA GACCTGAAGC CAGAGAACCT GCTGCTCGAC 
CACAACATGC ACGTGAAGAT CGCCGACTTT GGGCTCTCGA ACATGATGCT GGACGGCGAG 
TTCCTGCGCA CCTCGTGCGG CTCGCCCAAC TACGCGGCGC CCGAGGTGAT TTCCGGCAAG 
CTGTACGCCG GTCCCGAGGT GGACATCTGG TCGTGCGGCG TCATCCTGTA CGCCCTGCTG 
TGCGGGACGC TGCCCTTTGA CGACGAGCAT GTGCCTACGC TGTTTCGCAA GATCAAGTCG 
GGCATTTTCC CCATTCCCGA GTACCTCAAC AAACAAGTGG TCAATCTGGT CTGTCAGATG 
CTGCAAGTGG ACCCGCTCAA GCGGGCCAAC ATCGAGGAAA TCAAGAAGCA CGAGTGGTTC 
CAAAAGGACC TCCCGGCCTA CCTCTTCCCC TCGTCCATCG AGCAGGACTC CAATGTGATC 
GACACCTACG CTGTGGCAGA GGTCTGCACC AAGTTCGGCG TCAAGGAGAC CGAGGTGCAC 
AACTCGCTGC TCAGCGGCGA TCCGCACGAT CAGCTGGCCA TCGCTTACCA TCTGATCATC 
GACAACAAAC GCTTCGCCGA CGACGCGGCC AACCAAATAA ACGAGATCAA CAACTTCTTT 
GTGGCCGGCT CTCCACCACC GCCGCCGCCT CCGCCCGTTC CCCAATCATC GATGGACCAC 
CAAGCCCCTC TGGCCACCGT GACCGTTGGC GGAGGTACCT CAGCCAGCTC GGGCACCGCC 
ACACCAGTGC CACCAGTGGC TGGAGGCACG CCTAGCAGCA CCATACCCAT TCGACCGCAT 
CCGGAGCGGA TTGCGCCGAT GCGGGACCGT CAACTGGCCA TGTCCGTGCA GACATCTGGC 
GGCGGAGCCT TCCCGGAGAA AACCGCACGT GGCGGCACTC CCATCAAGCG CGCCAAGTGG 
CATCTGGGCA TCCGGTCGCA GAGCAAGCCC AACGACATAA TGCTCGAGGT ATACCGAGCG 
ATGAAGGCAC TCAGCTACGA GTGGAAGATC ATCAATCCGT ACCACGTTCG GGTGCGCAGG 
CAGAACGTGA AGACCGGCAA GTTCTCGAAG ATGTCACTGC AGCTGTACCA GGTGGACGCC 
AAGAGCTATC TGCTCGACTT CAAGTCGCTG ACCAACGACG AGGTCGAGCA GGGCGACGAC 
GTCATCATGG AGAGCCTTAC TCCGCCGCCG CTTAGCGTGT CCGGGGTGAT GCCGCTGCAG 
CCGACCGGCC ACCACACCAT GGAGTTTTTC GAAATGTGCG CCGCCCTGAT CATTCAACTG 
GCTCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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AMPKalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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