ras cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ras
Entrez Gene ID	43873
Full Name	raspberry
Synonyms	CG11485,CG1799,Dmel\CG1799,EP(X)1093,IMPDH,IMPdH,LD02673,LD06825,Ras,l(1)9Eb,l(1)G0002,l(1)G0056,l(1)G0098,l(1)G0127,l(1)G0238,l(1)G0351,l(1)G0380,l(1)G0388,l(1)G0391,l(1)G0436,l(1)G0482,ras-l,raspberry/impd
General protein information	Preferred Names raspberry Names CG1799 gene product from transcript CG1799-RD CG1799-PA CG1799-PC CG1799-PD CG1799-PE IMP dehydrogenase inosine monophosphate dehydrogenase lethal (1) G0380 ras-PA ras-PC ras-PD ras-PE raspberry-lethal
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 9E1-9E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_079907.7	NP_524646.5	raspberry, isoform E
NM_001272498.2	NP_001259427.1	raspberry, isoform D
NM_001369990.1	NP_001356940.1	raspberry, isoform F
NM_167241.3	NP_727442.1	raspberry, isoform C
NM_167240.3	NP_727441.1	raspberry, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	IMPDH1	NP_000874.2
	LOC701039	XP_002803509.1
Mus musculus (house mouse)	Impdh1	NP_035959.2
Danio rerio (zebrafish)	impdh1b	NP_001014391.1
Caenorhabditis elegans (roundworm)	T22D1.3	NP_001023395.1
Kluyveromyces lactis	KLLA0B05511g	XP_451781.1
Arabidopsis thaliana (thale cress)	AT1G79470	NP_178065.1
Oryza sativa (rice)	Os03g0780500	NP_001051450.1
Pan troglodytes (chimpanzee)	IMPDH1	XP_003951173.1
Rattus norvegicus (Norway rat)	Impdh1	NP_001102089.1
Danio rerio (zebrafish)	impdh1a	NP_001002177.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011133	XP_309514.2
Saccharomyces cerevisiae (baker's yeast)	IMD2	NP_012088.3
Eremothecium gossypii	AGOS_AER117W	NP_984977.2
Xenopus tropicalis (tropical clawed frog)	impdh1	NP_001017283.1
Bos taurus (cattle)	IMPDH1	NP_001071309.1
Saccharomyces cerevisiae (baker's yeast)	IMD4	NP_013656.1
Magnaporthe oryzae (rice blast fungus)	MGG_03699	XP_003716182.1
Schizosaccharomyces pombe (fission yeast)	gua1	NP_595702.1
Neurospora crassa	NCU03117	XP_964976.1
Canis lupus familiaris (dog)	IMPDH1	XP_003639517.2
Drosophila melanogaster (fruit fly)	ras	NP_001259427.1
Saccharomyces cerevisiae (baker's yeast)	IMD3	NP_013536.3
Arabidopsis thaliana (thale cress)	AT1G16350	NP_173085.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila inosine 5'-monophosphate dehydrogenase (IMPDH), encoded by raspberry (ras), is centrally important for a proper cellular immune response against eggs from the parasitoid wasp Leptopilina boulardi
Title: The raspberry Gene Is Involved in the Regulation of the Cellular Immune Response in Drosophila melanogaster.

Ras-Dsor1 activity is independent of upstream activation by EGFR, and instead it appears to be activated by the insulin-like growth factor receptor to promote Wg signaling.
Title: Ras-activated Dsor1 promotes Wnt signaling in Drosophila development.

Data show that inosine monophosphate dehydrogenase (IMPDH) binds sequence specifically to single-stranded, CT-rich DNA elements.
Title: Metabolic enzyme IMPDH is also a transcription factor regulated by cellular state.

Loss-of-function mutations in dPLZF resulted in a dramatic suppression of the extra and ectopic vein formation induced by elevated Ras/ERK signaling.
Title: The BTB/POZ-ZF transcription factor dPLZF is involved in Ras/ERK signaling during Drosophila wing development.

Ectopic Ras/MAPK pathway activation results in the suppression of Drosophila IMD/NF-kappaB signalling.
Title: Drosophila Ras/MAPK signalling regulates innate immune responses in immune and intestinal stem cells.

The following ras gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ras cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02289	NM_167241.3 Latest version!	Drosophila melanogaster raspberry (ras), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02289	NM_001272498.2 Latest version!	Drosophila melanogaster raspberry (ras), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02289	NM_079907.7 Latest version!	Drosophila melanogaster raspberry (ras), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02289	NM_167240.3 Latest version!	Drosophila melanogaster raspberry (ras), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa87671	NM_001369990.1 Latest version!	Drosophila melanogaster raspberry (ras), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ras ( NM_079907.7 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524646.5 Drosophila melanogaster raspberry (ras), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa02289
Clone ID Related Accession (Same CDS sequence)	NM_079907.7 , NM_001272498.2 , NM_167241.3 , NM_167240.3
Accession Version	NM_079907.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	raspberry, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_079907.6. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGCA CGACAAAGGT GAAGGTGAAC GGCTTTGTGG AATCGACGTC GTCTTCAGCG 
GCGCCGGCAA TCCAGACAAA GAGTACCACC GGATTCGATG CCGAGCTGCA GGATGGGCTG 
AGTTGTAAGG AACTGTTCCA GAACGGTGAG GGACTCACCT ACAACGACTT TCTCATACTG 
CCCGGCTACA TAGACTTCAC CGCCGAGGAG GTCGATCTCA GTTCGCCACT GACCAAGTCG 
CTGACATTGC GAGCACCGCT GGTTAGTTCG CCCATGGACA CGGTAACCGA ATCGGAGATG 
GCCATCGCCA TGGCGCTGTG TGGTGGCATT GGCATCATCC ATCACAACTG CACGCCGGAA 
TACCAGGCGT TGGAGGTGCA CAAGGTTAAG AAGTACAAGC ACGGCTTCAT GCGCGACCCC 
TCGGTGATGT CGCCCACGAA TACGGTGGGG GATGTGTTGG AGGCGCGGCG GAAGAACGGA 
TTCACCGGCT ATCCGGTCAC CGAGAACGGC AAACTTGGCG GCAAGCTGCT GGGCATGGTC 
ACGTCGCGAG ACATTGACTT CCGCGAGAAC CAGCCGGAGG TCCTGCTGGC CGACATCATG 
ACCACCGAAC TGGTCACCGC TCCCAATGGC ATCAATCTGC CCACGGCAAA CGCTATTCTC 
GAGAAGAGCA AGAAGGGCAA ACTGCCGATT GTGAATCAGG CCGGCGAACT GGTGGCCATG 
ATTGCCCGCA CGGATTTGAA GAAGGCCCGC TCCTACCCGA ATGCCTCCAA GGACTCCAAT 
AAGCAGCTCC TCGTCGGCGC TGCCATCGGA ACGCGATCGG AGGACAAGGC GCGCCTGGCT 
CTGCTGGTGG CCAACGGCGT GGACGTTATC ATCCTTGATT CATCGCAGGG CAACTCCGTC 
TACCAGGTGG AGATGATCAA GTACATTAAG GAGACCTATC CCGAACTACA GGTCATTGGT 
GGCAATGTGG TAACACGTGC CCAGGCCAAA AATCTCATTG ATGCCGGCGT CGATGGACTG 
CGTGTGGGCA TGGGTTCCGG TTCCATCTGC ATCACCCAGG AGGTGATGGC CTGCGGATGT 
CCTCAGGCCA CTGCTGTCTA CCAGGTGTCC ACGTACGCTC GCCAATTCGG AGTGCCAGTG 
ATCGCGGACG GAGGCATCCA GTCCATTGGA CACATTGTGA AGGCTATTGC ATTGGGAGCC 
AGCGCTGTAA TGATGGGCTC CCTGCTGGCG GGCACATCGG AGGCACCGGG CGAGTACTTC 
TTCTCCGACG GAGTGCGCCT TAAGAAGTAC CGCGGCATGG GCTCCCTGGA GGCCATGGAG 
CGCGGCGATG CCAAGGGCGC TGCCATGTCG CGCTACTACC ACAACGAGAT GGATAAAATG 
AAGGTGGCAC AGGGCGTCAG TGGCAGCATC GTGGACAAGG GCAGTGTGCT GCGCTATCTA 
CCCTATTTGG AGTGCGGACT GCAGCACAGT TGTCAGGACA TCGGTGCTAA CTCCATTAAT 
AAATTGAGGG ATATGATCTA TAATGGCCAA CTGCGCTTCA TGAAGCGCAC CCATTCCGCT 
CAACTGGAAG GCAATGTCCA TGGCCTCTTC AGCTACGAGA AGCGTCTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524646.5
CDS	199..1812
Translation	MESTTKVKVNGFVESTSSSAAPAIQTKSTTGFDAELQDGLSCKELFQNGEGLTYNDFLILPGYIDFTAEEVDLSSPLTKSLTLRAPLVSSPMDTVTESEMAIAMALCGGIGIIHHNCTPEYQALEVHKVKKYKHGFMRDPSVMSPTNTVGDVLEARRKNGFTGYPVTENGKLGGKLLGMVTSRDIDFRENQPEVLLADIMTTELVTAPNGINLPTANAILEKSKKGKLPIVNQAGELVAMIARTDLKKARSYPNASKDSNKQLLVGAAIGTRSEDKARLALLVANGVDVIILDSSQGNSVYQVEMIKYIKETYPELQVIGGNVVTRAQAKNLIDAGVDGLRVGMGSGSICITQEVMACGCPQATAVYQVSTYARQFGVPVIADGGIQSIGHIVKAIALGASAVMMGSLLAGTSEAPGEYFFSDGVRLKKYRGMGSLEAMERGDAKGAAMSRYYHNEMDKMKVAQGVSGSIVDKGSVLRYLPYLECGLQHSCQDIGANSINKLRDMIYNGQLRFMKRTHSAQLEGNVHGLFSYEKRLF

Target ORF information:

RefSeq Version	NM_079907.7
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster raspberry (ras), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079907.7

ORF Insert Sequence:

ATGGAGAGCA CGACAAAGGT GAAGGTGAAC GGCTTTGTGG AATCGACGTC GTCTTCAGCG 
GCGCCGGCAA TCCAGACAAA GAGTACCACC GGATTCGATG CCGAGCTGCA GGATGGGCTG 
AGTTGTAAGG AACTGTTCCA GAACGGTGAG GGACTCACCT ACAACGACTT TCTCATACTG 
CCCGGCTACA TAGACTTCAC CGCCGAGGAG GTCGATCTCA GTTCGCCACT GACCAAGTCG 
CTGACATTGC GAGCACCGCT GGTTAGTTCG CCCATGGACA CGGTAACCGA ATCGGAGATG 
GCCATCGCCA TGGCGCTGTG TGGTGGCATT GGCATCATCC ATCACAACTG CACGCCGGAA 
TACCAGGCGT TGGAGGTGCA CAAGGTTAAG AAGTACAAGC ACGGCTTCAT GCGCGACCCC 
TCGGTGATGT CGCCCACGAA TACGGTGGGG GATGTGTTGG AGGCGCGGCG GAAGAACGGA 
TTCACCGGCT ATCCGGTCAC CGAGAACGGC AAACTTGGCG GCAAGCTGCT GGGCATGGTC 
ACGTCGCGAG ACATTGACTT CCGCGAGAAC CAGCCGGAGG TCCTGCTGGC CGACATCATG 
ACCACCGAAC TGGTCACCGC TCCCAATGGC ATCAATCTGC CCACGGCAAA CGCTATTCTC 
GAGAAGAGCA AGAAGGGCAA ACTGCCGATT GTGAATCAGG CCGGCGAACT GGTGGCCATG 
ATTGCCCGCA CGGATTTGAA GAAGGCCCGC TCCTACCCGA ATGCCTCCAA GGACTCCAAT 
AAGCAGCTCC TCGTCGGCGC TGCCATCGGA ACGCGATCGG AGGACAAGGC GCGCCTGGCT 
CTGCTGGTGG CCAACGGCGT GGACGTTATC ATCCTTGATT CATCGCAGGG CAACTCCGTC 
TACCAGGTGG AGATGATCAA GTACATTAAG GAGACCTATC CCGAACTACA GGTCATTGGT 
GGCAATGTGG TAACACGTGC CCAGGCCAAA AATCTCATTG ATGCCGGCGT CGATGGACTG 
CGTGTGGGCA TGGGTTCCGG TTCCATCTGC ATCACCCAGG AGGTGATGGC CTGCGGATGT 
CCTCAGGCCA CTGCTGTCTA CCAGGTGTCC ACGTACGCTC GCCAATTCGG AGTGCCAGTG 
ATCGCGGACG GAGGCATCCA GTCCATTGGA CACATTGTGA AGGCTATTGC ATTGGGAGCC 
AGCGCTGTAA TGATGGGCTC CCTGCTGGCG GGCACATCGG AGGCACCGGG CGAGTACTTC 
TTCTCCGACG GAGTGCGCCT TAAGAAGTAC CGCGGCATGG GCTCCCTGGA GGCCATGGAG 
CGCGGCGATG CCAAGGGCGC TGCCATGTCG CGCTACTACC ACAACGAGAT GGATAAAATG 
AAGGTGGCAC AGGGCGTCAG TGGCAGCATC GTGGACAAGG GCAGTGTGCT GCGCTATCTA 
CCCTATTTGG AGTGCGGACT GCAGCACAGT TGTCAGGACA TCGGTGCTAA CTCCATTAAT 
AAATTGAGGG ATATGATCTA TAATGGCCAA CTGCGCTTCA TGAAGCGCAC CCATTCCGCT 
CAACTGGAAG GCAATGTCCA TGGCCTCTTC AGCTACGAGA AGCGTCTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ras ( NM_001272498.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259427.1 Drosophila melanogaster raspberry (ras), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa02289
Clone ID Related Accession (Same CDS sequence)	NM_079907.7 , NM_001272498.2 , NM_167241.3 , NM_167240.3
Accession Version	NM_001272498.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	raspberry, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272498.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGCA CGACAAAGGT GAAGGTGAAC GGCTTTGTGG AATCGACGTC GTCTTCAGCG 
GCGCCGGCAA TCCAGACAAA GAGTACCACC GGATTCGATG CCGAGCTGCA GGATGGGCTG 
AGTTGTAAGG AACTGTTCCA GAACGGTGAG GGACTCACCT ACAACGACTT TCTCATACTG 
CCCGGCTACA TAGACTTCAC CGCCGAGGAG GTCGATCTCA GTTCGCCACT GACCAAGTCG 
CTGACATTGC GAGCACCGCT GGTTAGTTCG CCCATGGACA CGGTAACCGA ATCGGAGATG 
GCCATCGCCA TGGCGCTGTG TGGTGGCATT GGCATCATCC ATCACAACTG CACGCCGGAA 
TACCAGGCGT TGGAGGTGCA CAAGGTTAAG AAGTACAAGC ACGGCTTCAT GCGCGACCCC 
TCGGTGATGT CGCCCACGAA TACGGTGGGG GATGTGTTGG AGGCGCGGCG GAAGAACGGA 
TTCACCGGCT ATCCGGTCAC CGAGAACGGC AAACTTGGCG GCAAGCTGCT GGGCATGGTC 
ACGTCGCGAG ACATTGACTT CCGCGAGAAC CAGCCGGAGG TCCTGCTGGC CGACATCATG 
ACCACCGAAC TGGTCACCGC TCCCAATGGC ATCAATCTGC CCACGGCAAA CGCTATTCTC 
GAGAAGAGCA AGAAGGGCAA ACTGCCGATT GTGAATCAGG CCGGCGAACT GGTGGCCATG 
ATTGCCCGCA CGGATTTGAA GAAGGCCCGC TCCTACCCGA ATGCCTCCAA GGACTCCAAT 
AAGCAGCTCC TCGTCGGCGC TGCCATCGGA ACGCGATCGG AGGACAAGGC GCGCCTGGCT 
CTGCTGGTGG CCAACGGCGT GGACGTTATC ATCCTTGATT CATCGCAGGG CAACTCCGTC 
TACCAGGTGG AGATGATCAA GTACATTAAG GAGACCTATC CCGAACTACA GGTCATTGGT 
GGCAATGTGG TAACACGTGC CCAGGCCAAA AATCTCATTG ATGCCGGCGT CGATGGACTG 
CGTGTGGGCA TGGGTTCCGG TTCCATCTGC ATCACCCAGG AGGTGATGGC CTGCGGATGT 
CCTCAGGCCA CTGCTGTCTA CCAGGTGTCC ACGTACGCTC GCCAATTCGG AGTGCCAGTG 
ATCGCGGACG GAGGCATCCA GTCCATTGGA CACATTGTGA AGGCTATTGC ATTGGGAGCC 
AGCGCTGTAA TGATGGGCTC CCTGCTGGCG GGCACATCGG AGGCACCGGG CGAGTACTTC 
TTCTCCGACG GAGTGCGCCT TAAGAAGTAC CGCGGCATGG GCTCCCTGGA GGCCATGGAG 
CGCGGCGATG CCAAGGGCGC TGCCATGTCG CGCTACTACC ACAACGAGAT GGATAAAATG 
AAGGTGGCAC AGGGCGTCAG TGGCAGCATC GTGGACAAGG GCAGTGTGCT GCGCTATCTA 
CCCTATTTGG AGTGCGGACT GCAGCACAGT TGTCAGGACA TCGGTGCTAA CTCCATTAAT 
AAATTGAGGG ATATGATCTA TAATGGCCAA CTGCGCTTCA TGAAGCGCAC CCATTCCGCT 
CAACTGGAAG GCAATGTCCA TGGCCTCTTC AGCTACGAGA AGCGTCTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259427.1
CDS	303..1916
Translation	MESTTKVKVNGFVESTSSSAAPAIQTKSTTGFDAELQDGLSCKELFQNGEGLTYNDFLILPGYIDFTAEEVDLSSPLTKSLTLRAPLVSSPMDTVTESEMAIAMALCGGIGIIHHNCTPEYQALEVHKVKKYKHGFMRDPSVMSPTNTVGDVLEARRKNGFTGYPVTENGKLGGKLLGMVTSRDIDFRENQPEVLLADIMTTELVTAPNGINLPTANAILEKSKKGKLPIVNQAGELVAMIARTDLKKARSYPNASKDSNKQLLVGAAIGTRSEDKARLALLVANGVDVIILDSSQGNSVYQVEMIKYIKETYPELQVIGGNVVTRAQAKNLIDAGVDGLRVGMGSGSICITQEVMACGCPQATAVYQVSTYARQFGVPVIADGGIQSIGHIVKAIALGASAVMMGSLLAGTSEAPGEYFFSDGVRLKKYRGMGSLEAMERGDAKGAAMSRYYHNEMDKMKVAQGVSGSIVDKGSVLRYLPYLECGLQHSCQDIGANSINKLRDMIYNGQLRFMKRTHSAQLEGNVHGLFSYEKRLF

Target ORF information:

RefSeq Version	NM_001272498.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster raspberry (ras), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272498.2

ORF Insert Sequence:

ATGGAGAGCA CGACAAAGGT GAAGGTGAAC GGCTTTGTGG AATCGACGTC GTCTTCAGCG 
GCGCCGGCAA TCCAGACAAA GAGTACCACC GGATTCGATG CCGAGCTGCA GGATGGGCTG 
AGTTGTAAGG AACTGTTCCA GAACGGTGAG GGACTCACCT ACAACGACTT TCTCATACTG 
CCCGGCTACA TAGACTTCAC CGCCGAGGAG GTCGATCTCA GTTCGCCACT GACCAAGTCG 
CTGACATTGC GAGCACCGCT GGTTAGTTCG CCCATGGACA CGGTAACCGA ATCGGAGATG 
GCCATCGCCA TGGCGCTGTG TGGTGGCATT GGCATCATCC ATCACAACTG CACGCCGGAA 
TACCAGGCGT TGGAGGTGCA CAAGGTTAAG AAGTACAAGC ACGGCTTCAT GCGCGACCCC 
TCGGTGATGT CGCCCACGAA TACGGTGGGG GATGTGTTGG AGGCGCGGCG GAAGAACGGA 
TTCACCGGCT ATCCGGTCAC CGAGAACGGC AAACTTGGCG GCAAGCTGCT GGGCATGGTC 
ACGTCGCGAG ACATTGACTT CCGCGAGAAC CAGCCGGAGG TCCTGCTGGC CGACATCATG 
ACCACCGAAC TGGTCACCGC TCCCAATGGC ATCAATCTGC CCACGGCAAA CGCTATTCTC 
GAGAAGAGCA AGAAGGGCAA ACTGCCGATT GTGAATCAGG CCGGCGAACT GGTGGCCATG 
ATTGCCCGCA CGGATTTGAA GAAGGCCCGC TCCTACCCGA ATGCCTCCAA GGACTCCAAT 
AAGCAGCTCC TCGTCGGCGC TGCCATCGGA ACGCGATCGG AGGACAAGGC GCGCCTGGCT 
CTGCTGGTGG CCAACGGCGT GGACGTTATC ATCCTTGATT CATCGCAGGG CAACTCCGTC 
TACCAGGTGG AGATGATCAA GTACATTAAG GAGACCTATC CCGAACTACA GGTCATTGGT 
GGCAATGTGG TAACACGTGC CCAGGCCAAA AATCTCATTG ATGCCGGCGT CGATGGACTG 
CGTGTGGGCA TGGGTTCCGG TTCCATCTGC ATCACCCAGG AGGTGATGGC CTGCGGATGT 
CCTCAGGCCA CTGCTGTCTA CCAGGTGTCC ACGTACGCTC GCCAATTCGG AGTGCCAGTG 
ATCGCGGACG GAGGCATCCA GTCCATTGGA CACATTGTGA AGGCTATTGC ATTGGGAGCC 
AGCGCTGTAA TGATGGGCTC CCTGCTGGCG GGCACATCGG AGGCACCGGG CGAGTACTTC 
TTCTCCGACG GAGTGCGCCT TAAGAAGTAC CGCGGCATGG GCTCCCTGGA GGCCATGGAG 
CGCGGCGATG CCAAGGGCGC TGCCATGTCG CGCTACTACC ACAACGAGAT GGATAAAATG 
AAGGTGGCAC AGGGCGTCAG TGGCAGCATC GTGGACAAGG GCAGTGTGCT GCGCTATCTA 
CCCTATTTGG AGTGCGGACT GCAGCACAGT TGTCAGGACA TCGGTGCTAA CTCCATTAAT 
AAATTGAGGG ATATGATCTA TAATGGCCAA CTGCGCTTCA TGAAGCGCAC CCATTCCGCT 
CAACTGGAAG GCAATGTCCA TGGCCTCTTC AGCTACGAGA AGCGTCTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ras ( NM_167241.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727442.1 Drosophila melanogaster raspberry (ras), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa02289
Clone ID Related Accession (Same CDS sequence)	NM_079907.7 , NM_001272498.2 , NM_167241.3 , NM_167240.3
Accession Version	NM_167241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	raspberry, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167241.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGCA CGACAAAGGT GAAGGTGAAC GGCTTTGTGG AATCGACGTC GTCTTCAGCG 
GCGCCGGCAA TCCAGACAAA GAGTACCACC GGATTCGATG CCGAGCTGCA GGATGGGCTG 
AGTTGTAAGG AACTGTTCCA GAACGGTGAG GGACTCACCT ACAACGACTT TCTCATACTG 
CCCGGCTACA TAGACTTCAC CGCCGAGGAG GTCGATCTCA GTTCGCCACT GACCAAGTCG 
CTGACATTGC GAGCACCGCT GGTTAGTTCG CCCATGGACA CGGTAACCGA ATCGGAGATG 
GCCATCGCCA TGGCGCTGTG TGGTGGCATT GGCATCATCC ATCACAACTG CACGCCGGAA 
TACCAGGCGT TGGAGGTGCA CAAGGTTAAG AAGTACAAGC ACGGCTTCAT GCGCGACCCC 
TCGGTGATGT CGCCCACGAA TACGGTGGGG GATGTGTTGG AGGCGCGGCG GAAGAACGGA 
TTCACCGGCT ATCCGGTCAC CGAGAACGGC AAACTTGGCG GCAAGCTGCT GGGCATGGTC 
ACGTCGCGAG ACATTGACTT CCGCGAGAAC CAGCCGGAGG TCCTGCTGGC CGACATCATG 
ACCACCGAAC TGGTCACCGC TCCCAATGGC ATCAATCTGC CCACGGCAAA CGCTATTCTC 
GAGAAGAGCA AGAAGGGCAA ACTGCCGATT GTGAATCAGG CCGGCGAACT GGTGGCCATG 
ATTGCCCGCA CGGATTTGAA GAAGGCCCGC TCCTACCCGA ATGCCTCCAA GGACTCCAAT 
AAGCAGCTCC TCGTCGGCGC TGCCATCGGA ACGCGATCGG AGGACAAGGC GCGCCTGGCT 
CTGCTGGTGG CCAACGGCGT GGACGTTATC ATCCTTGATT CATCGCAGGG CAACTCCGTC 
TACCAGGTGG AGATGATCAA GTACATTAAG GAGACCTATC CCGAACTACA GGTCATTGGT 
GGCAATGTGG TAACACGTGC CCAGGCCAAA AATCTCATTG ATGCCGGCGT CGATGGACTG 
CGTGTGGGCA TGGGTTCCGG TTCCATCTGC ATCACCCAGG AGGTGATGGC CTGCGGATGT 
CCTCAGGCCA CTGCTGTCTA CCAGGTGTCC ACGTACGCTC GCCAATTCGG AGTGCCAGTG 
ATCGCGGACG GAGGCATCCA GTCCATTGGA CACATTGTGA AGGCTATTGC ATTGGGAGCC 
AGCGCTGTAA TGATGGGCTC CCTGCTGGCG GGCACATCGG AGGCACCGGG CGAGTACTTC 
TTCTCCGACG GAGTGCGCCT TAAGAAGTAC CGCGGCATGG GCTCCCTGGA GGCCATGGAG 
CGCGGCGATG CCAAGGGCGC TGCCATGTCG CGCTACTACC ACAACGAGAT GGATAAAATG 
AAGGTGGCAC AGGGCGTCAG TGGCAGCATC GTGGACAAGG GCAGTGTGCT GCGCTATCTA 
CCCTATTTGG AGTGCGGACT GCAGCACAGT TGTCAGGACA TCGGTGCTAA CTCCATTAAT 
AAATTGAGGG ATATGATCTA TAATGGCCAA CTGCGCTTCA TGAAGCGCAC CCATTCCGCT 
CAACTGGAAG GCAATGTCCA TGGCCTCTTC AGCTACGAGA AGCGTCTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727442.1
CDS	105..1718
Translation	MESTTKVKVNGFVESTSSSAAPAIQTKSTTGFDAELQDGLSCKELFQNGEGLTYNDFLILPGYIDFTAEEVDLSSPLTKSLTLRAPLVSSPMDTVTESEMAIAMALCGGIGIIHHNCTPEYQALEVHKVKKYKHGFMRDPSVMSPTNTVGDVLEARRKNGFTGYPVTENGKLGGKLLGMVTSRDIDFRENQPEVLLADIMTTELVTAPNGINLPTANAILEKSKKGKLPIVNQAGELVAMIARTDLKKARSYPNASKDSNKQLLVGAAIGTRSEDKARLALLVANGVDVIILDSSQGNSVYQVEMIKYIKETYPELQVIGGNVVTRAQAKNLIDAGVDGLRVGMGSGSICITQEVMACGCPQATAVYQVSTYARQFGVPVIADGGIQSIGHIVKAIALGASAVMMGSLLAGTSEAPGEYFFSDGVRLKKYRGMGSLEAMERGDAKGAAMSRYYHNEMDKMKVAQGVSGSIVDKGSVLRYLPYLECGLQHSCQDIGANSINKLRDMIYNGQLRFMKRTHSAQLEGNVHGLFSYEKRLF

Target ORF information:

RefSeq Version	NM_167241.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster raspberry (ras), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167241.3

ORF Insert Sequence:

ATGGAGAGCA CGACAAAGGT GAAGGTGAAC GGCTTTGTGG AATCGACGTC GTCTTCAGCG 
GCGCCGGCAA TCCAGACAAA GAGTACCACC GGATTCGATG CCGAGCTGCA GGATGGGCTG 
AGTTGTAAGG AACTGTTCCA GAACGGTGAG GGACTCACCT ACAACGACTT TCTCATACTG 
CCCGGCTACA TAGACTTCAC CGCCGAGGAG GTCGATCTCA GTTCGCCACT GACCAAGTCG 
CTGACATTGC GAGCACCGCT GGTTAGTTCG CCCATGGACA CGGTAACCGA ATCGGAGATG 
GCCATCGCCA TGGCGCTGTG TGGTGGCATT GGCATCATCC ATCACAACTG CACGCCGGAA 
TACCAGGCGT TGGAGGTGCA CAAGGTTAAG AAGTACAAGC ACGGCTTCAT GCGCGACCCC 
TCGGTGATGT CGCCCACGAA TACGGTGGGG GATGTGTTGG AGGCGCGGCG GAAGAACGGA 
TTCACCGGCT ATCCGGTCAC CGAGAACGGC AAACTTGGCG GCAAGCTGCT GGGCATGGTC 
ACGTCGCGAG ACATTGACTT CCGCGAGAAC CAGCCGGAGG TCCTGCTGGC CGACATCATG 
ACCACCGAAC TGGTCACCGC TCCCAATGGC ATCAATCTGC CCACGGCAAA CGCTATTCTC 
GAGAAGAGCA AGAAGGGCAA ACTGCCGATT GTGAATCAGG CCGGCGAACT GGTGGCCATG 
ATTGCCCGCA CGGATTTGAA GAAGGCCCGC TCCTACCCGA ATGCCTCCAA GGACTCCAAT 
AAGCAGCTCC TCGTCGGCGC TGCCATCGGA ACGCGATCGG AGGACAAGGC GCGCCTGGCT 
CTGCTGGTGG CCAACGGCGT GGACGTTATC ATCCTTGATT CATCGCAGGG CAACTCCGTC 
TACCAGGTGG AGATGATCAA GTACATTAAG GAGACCTATC CCGAACTACA GGTCATTGGT 
GGCAATGTGG TAACACGTGC CCAGGCCAAA AATCTCATTG ATGCCGGCGT CGATGGACTG 
CGTGTGGGCA TGGGTTCCGG TTCCATCTGC ATCACCCAGG AGGTGATGGC CTGCGGATGT 
CCTCAGGCCA CTGCTGTCTA CCAGGTGTCC ACGTACGCTC GCCAATTCGG AGTGCCAGTG 
ATCGCGGACG GAGGCATCCA GTCCATTGGA CACATTGTGA AGGCTATTGC ATTGGGAGCC 
AGCGCTGTAA TGATGGGCTC CCTGCTGGCG GGCACATCGG AGGCACCGGG CGAGTACTTC 
TTCTCCGACG GAGTGCGCCT TAAGAAGTAC CGCGGCATGG GCTCCCTGGA GGCCATGGAG 
CGCGGCGATG CCAAGGGCGC TGCCATGTCG CGCTACTACC ACAACGAGAT GGATAAAATG 
AAGGTGGCAC AGGGCGTCAG TGGCAGCATC GTGGACAAGG GCAGTGTGCT GCGCTATCTA 
CCCTATTTGG AGTGCGGACT GCAGCACAGT TGTCAGGACA TCGGTGCTAA CTCCATTAAT 
AAATTGAGGG ATATGATCTA TAATGGCCAA CTGCGCTTCA TGAAGCGCAC CCATTCCGCT 
CAACTGGAAG GCAATGTCCA TGGCCTCTTC AGCTACGAGA AGCGTCTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ras ( NM_167240.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727441.1 Drosophila melanogaster raspberry (ras), transcript variant A, mRNA.

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CloneID	OFa02289
Clone ID Related Accession (Same CDS sequence)	NM_079907.7 , NM_001272498.2 , NM_167241.3 , NM_167240.3
Accession Version	NM_167240.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	raspberry, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167240.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGCA CGACAAAGGT GAAGGTGAAC GGCTTTGTGG AATCGACGTC GTCTTCAGCG 
GCGCCGGCAA TCCAGACAAA GAGTACCACC GGATTCGATG CCGAGCTGCA GGATGGGCTG 
AGTTGTAAGG AACTGTTCCA GAACGGTGAG GGACTCACCT ACAACGACTT TCTCATACTG 
CCCGGCTACA TAGACTTCAC CGCCGAGGAG GTCGATCTCA GTTCGCCACT GACCAAGTCG 
CTGACATTGC GAGCACCGCT GGTTAGTTCG CCCATGGACA CGGTAACCGA ATCGGAGATG 
GCCATCGCCA TGGCGCTGTG TGGTGGCATT GGCATCATCC ATCACAACTG CACGCCGGAA 
TACCAGGCGT TGGAGGTGCA CAAGGTTAAG AAGTACAAGC ACGGCTTCAT GCGCGACCCC 
TCGGTGATGT CGCCCACGAA TACGGTGGGG GATGTGTTGG AGGCGCGGCG GAAGAACGGA 
TTCACCGGCT ATCCGGTCAC CGAGAACGGC AAACTTGGCG GCAAGCTGCT GGGCATGGTC 
ACGTCGCGAG ACATTGACTT CCGCGAGAAC CAGCCGGAGG TCCTGCTGGC CGACATCATG 
ACCACCGAAC TGGTCACCGC TCCCAATGGC ATCAATCTGC CCACGGCAAA CGCTATTCTC 
GAGAAGAGCA AGAAGGGCAA ACTGCCGATT GTGAATCAGG CCGGCGAACT GGTGGCCATG 
ATTGCCCGCA CGGATTTGAA GAAGGCCCGC TCCTACCCGA ATGCCTCCAA GGACTCCAAT 
AAGCAGCTCC TCGTCGGCGC TGCCATCGGA ACGCGATCGG AGGACAAGGC GCGCCTGGCT 
CTGCTGGTGG CCAACGGCGT GGACGTTATC ATCCTTGATT CATCGCAGGG CAACTCCGTC 
TACCAGGTGG AGATGATCAA GTACATTAAG GAGACCTATC CCGAACTACA GGTCATTGGT 
GGCAATGTGG TAACACGTGC CCAGGCCAAA AATCTCATTG ATGCCGGCGT CGATGGACTG 
CGTGTGGGCA TGGGTTCCGG TTCCATCTGC ATCACCCAGG AGGTGATGGC CTGCGGATGT 
CCTCAGGCCA CTGCTGTCTA CCAGGTGTCC ACGTACGCTC GCCAATTCGG AGTGCCAGTG 
ATCGCGGACG GAGGCATCCA GTCCATTGGA CACATTGTGA AGGCTATTGC ATTGGGAGCC 
AGCGCTGTAA TGATGGGCTC CCTGCTGGCG GGCACATCGG AGGCACCGGG CGAGTACTTC 
TTCTCCGACG GAGTGCGCCT TAAGAAGTAC CGCGGCATGG GCTCCCTGGA GGCCATGGAG 
CGCGGCGATG CCAAGGGCGC TGCCATGTCG CGCTACTACC ACAACGAGAT GGATAAAATG 
AAGGTGGCAC AGGGCGTCAG TGGCAGCATC GTGGACAAGG GCAGTGTGCT GCGCTATCTA 
CCCTATTTGG AGTGCGGACT GCAGCACAGT TGTCAGGACA TCGGTGCTAA CTCCATTAAT 
AAATTGAGGG ATATGATCTA TAATGGCCAA CTGCGCTTCA TGAAGCGCAC CCATTCCGCT 
CAACTGGAAG GCAATGTCCA TGGCCTCTTC AGCTACGAGA AGCGTCTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727441.1
CDS	303..1916
Translation	MESTTKVKVNGFVESTSSSAAPAIQTKSTTGFDAELQDGLSCKELFQNGEGLTYNDFLILPGYIDFTAEEVDLSSPLTKSLTLRAPLVSSPMDTVTESEMAIAMALCGGIGIIHHNCTPEYQALEVHKVKKYKHGFMRDPSVMSPTNTVGDVLEARRKNGFTGYPVTENGKLGGKLLGMVTSRDIDFRENQPEVLLADIMTTELVTAPNGINLPTANAILEKSKKGKLPIVNQAGELVAMIARTDLKKARSYPNASKDSNKQLLVGAAIGTRSEDKARLALLVANGVDVIILDSSQGNSVYQVEMIKYIKETYPELQVIGGNVVTRAQAKNLIDAGVDGLRVGMGSGSICITQEVMACGCPQATAVYQVSTYARQFGVPVIADGGIQSIGHIVKAIALGASAVMMGSLLAGTSEAPGEYFFSDGVRLKKYRGMGSLEAMERGDAKGAAMSRYYHNEMDKMKVAQGVSGSIVDKGSVLRYLPYLECGLQHSCQDIGANSINKLRDMIYNGQLRFMKRTHSAQLEGNVHGLFSYEKRLF

Target ORF information:

RefSeq Version	NM_167240.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster raspberry (ras), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167240.3

ORF Insert Sequence:

ATGGAGAGCA CGACAAAGGT GAAGGTGAAC GGCTTTGTGG AATCGACGTC GTCTTCAGCG 
GCGCCGGCAA TCCAGACAAA GAGTACCACC GGATTCGATG CCGAGCTGCA GGATGGGCTG 
AGTTGTAAGG AACTGTTCCA GAACGGTGAG GGACTCACCT ACAACGACTT TCTCATACTG 
CCCGGCTACA TAGACTTCAC CGCCGAGGAG GTCGATCTCA GTTCGCCACT GACCAAGTCG 
CTGACATTGC GAGCACCGCT GGTTAGTTCG CCCATGGACA CGGTAACCGA ATCGGAGATG 
GCCATCGCCA TGGCGCTGTG TGGTGGCATT GGCATCATCC ATCACAACTG CACGCCGGAA 
TACCAGGCGT TGGAGGTGCA CAAGGTTAAG AAGTACAAGC ACGGCTTCAT GCGCGACCCC 
TCGGTGATGT CGCCCACGAA TACGGTGGGG GATGTGTTGG AGGCGCGGCG GAAGAACGGA 
TTCACCGGCT ATCCGGTCAC CGAGAACGGC AAACTTGGCG GCAAGCTGCT GGGCATGGTC 
ACGTCGCGAG ACATTGACTT CCGCGAGAAC CAGCCGGAGG TCCTGCTGGC CGACATCATG 
ACCACCGAAC TGGTCACCGC TCCCAATGGC ATCAATCTGC CCACGGCAAA CGCTATTCTC 
GAGAAGAGCA AGAAGGGCAA ACTGCCGATT GTGAATCAGG CCGGCGAACT GGTGGCCATG 
ATTGCCCGCA CGGATTTGAA GAAGGCCCGC TCCTACCCGA ATGCCTCCAA GGACTCCAAT 
AAGCAGCTCC TCGTCGGCGC TGCCATCGGA ACGCGATCGG AGGACAAGGC GCGCCTGGCT 
CTGCTGGTGG CCAACGGCGT GGACGTTATC ATCCTTGATT CATCGCAGGG CAACTCCGTC 
TACCAGGTGG AGATGATCAA GTACATTAAG GAGACCTATC CCGAACTACA GGTCATTGGT 
GGCAATGTGG TAACACGTGC CCAGGCCAAA AATCTCATTG ATGCCGGCGT CGATGGACTG 
CGTGTGGGCA TGGGTTCCGG TTCCATCTGC ATCACCCAGG AGGTGATGGC CTGCGGATGT 
CCTCAGGCCA CTGCTGTCTA CCAGGTGTCC ACGTACGCTC GCCAATTCGG AGTGCCAGTG 
ATCGCGGACG GAGGCATCCA GTCCATTGGA CACATTGTGA AGGCTATTGC ATTGGGAGCC 
AGCGCTGTAA TGATGGGCTC CCTGCTGGCG GGCACATCGG AGGCACCGGG CGAGTACTTC 
TTCTCCGACG GAGTGCGCCT TAAGAAGTAC CGCGGCATGG GCTCCCTGGA GGCCATGGAG 
CGCGGCGATG CCAAGGGCGC TGCCATGTCG CGCTACTACC ACAACGAGAT GGATAAAATG 
AAGGTGGCAC AGGGCGTCAG TGGCAGCATC GTGGACAAGG GCAGTGTGCT GCGCTATCTA 
CCCTATTTGG AGTGCGGACT GCAGCACAGT TGTCAGGACA TCGGTGCTAA CTCCATTAAT 
AAATTGAGGG ATATGATCTA TAATGGCCAA CTGCGCTTCA TGAAGCGCAC CCATTCCGCT 
CAACTGGAAG GCAATGTCCA TGGCCTCTTC AGCTACGAGA AGCGTCTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ras ( NM_001369990.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356940.1 Drosophila melanogaster raspberry (ras), transcript variant F, mRNA.

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CloneID	OFa87671
Clone ID Related Accession (Same CDS sequence)	NM_001369990.1
Accession Version	NM_001369990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	raspberry, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGCA CGACAAAGGT GAAGGTGAAC GGCTTTGTGG AATCGACGTC GTCTTCAGCG 
GCGCCGGCAA TCCAGACAAA GAGTACCACC GGATTCGATG CCGAGCTGCA GGATGGGCTG 
AGTTGTAAGG AACTGTTCCA GAACGGTGAG GGACTCACCT ACAACGACTT TCTCATACTG 
CCCGGCTACA TAGACTTCAC CGCCGAGGAG GTCGATCTCA GTTCGCCACT GACCAAGTCG 
CTGACATTGC GAGCACCGCT GGTTAGTTCG CCCATGGACA CGGTAACCGA ATCGGAGATG 
GCCATCGCCA TGGCGCTGTG TGGTGGCATT GGCATCATCC ATCACAACTG CACGCCGGAA 
TACCAGGCGT TGGAGGTGCA CAAGGTTAAG AAGTACAAGC ACGGCTTCAT GCGCGACCCC 
TCGGTGATGT CGCCCACGAA TACGGTGGGG GATGTGTTGG AGGCGCGGCG GAAGAACGGA 
TTCACCGGCT ATCCGGTCAC CGAGAACGGC AAACTTGGCG GCAAGCTGCT GGGCATGGTC 
ACGTCGCGAG ACATTGACTT CCGCGAGAAC CAGCCGGAGG TCCTGCTGGC CGACATCATG 
ACCACCGAAC TGGTCACCGC TCCCAATGGC ATCAATCTGC CCACGGCAAA CGCTATTCTC 
GAGAAGAGCA AGAAGGGCAA ACTGCCGATT GTGAATCAGG CCGGCGAACT GGTGGCCATG 
ATTGCCCGCA CGGATTTGAA GAAGGCCCGC TCCTACCCGA ATGCCTCCAA GGACTCCAAT 
AAGCAGCTCC TCGTCGGCGC TGCCATCGGA ACGCGATCGG AGGACAAGGC GCGCCTGGCT 
CTGCTGGTGG CCAACGGCGT GGACGTTATC ATCCTTGATT CATCGCAGGG CAACTCCGTC 
TACCAGGTGG AGATGATCAA GTACATTAAG GAGACCTATC CCGAACTACA GGTCATTGGT 
GGCAATGTGG TAACACGTGC CCAGGCCAAA AATCTCATTG ATGCCGGCGT CGATGGACTG 
CGTGTGGGCA TGGGTTCCGG TTCCATCTGC ATCACCCAGG AGGTGATGGC CTGCGGATGT 
CCTCAGGCCA CTGCTGTCTA CCAGGTGTCC ACGTACGCTC GCCAATTCGG AGTGCCAGTG 
ATCGCGGACG GAGGCATCCA GTCCATTGGA CACATTGTGA AGGCTATTGC ATTGGGAGCC 
AGCGCTGTAA TGATGGGCTC CCTGCTGGCG GGCACATCGG AGGCACCGGG CGAGTACTTC 
TTCTCCGACG GAGTGCGCCT TAAGAAGTAC CGCGGCATGG GCTCCCTGGA GGCCATGGAG 
CGCGGCGATG CCAAGGGCGC TGCCATGTCG CGCTACTACC ACAACGAGAT GGATAAAATG 
AAGGTGGCAC AGGGCGTCAG TGGCAGCATC GTGGACAAGG GCAGTGTGCT GCGCTATCTA 
CCCTATTTGG AGTGCGGACT GCAGCACAGT TGTCAGGACA TCGGTGCTAA CTCCATTAAT 
AAATTGAGGG ATATGATCTA TAATGGCCAA CTGCGCTTCA TGAAGCGCAC CCATTCCGCT 
CAACTGGAAG GCAATGTCCA TGGCCTCTTC AGCTACGAGA AGCGTCTCTT CTAACATACG 
ACGATGGCTG CGACGGAAAC GGGCAGTGGA GGAGTGCCTG CCCCTGTCCA GCAGCAGCAG 
CAGCAGCAGC AACACCATCA GCAGCAACAG CATCGCCACC GATCTGCAGC AGGCCCACTG 
CTCGACTACA CCCGCCTATT CGATCGCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356940.1
CDS	199..1971
Translation	MESTTKVKVNGFVESTSSSAAPAIQTKSTTGFDAELQDGLSCKELFQNGEGLTYNDFLILPGYIDFTAEEVDLSSPLTKSLTLRAPLVSSPMDTVTESEMAIAMALCGGIGIIHHNCTPEYQALEVHKVKKYKHGFMRDPSVMSPTNTVGDVLEARRKNGFTGYPVTENGKLGGKLLGMVTSRDIDFRENQPEVLLADIMTTELVTAPNGINLPTANAILEKSKKGKLPIVNQAGELVAMIARTDLKKARSYPNASKDSNKQLLVGAAIGTRSEDKARLALLVANGVDVIILDSSQGNSVYQVEMIKYIKETYPELQVIGGNVVTRAQAKNLIDAGVDGLRVGMGSGSICITQEVMACGCPQATAVYQVSTYARQFGVPVIADGGIQSIGHIVKAIALGASAVMMGSLLAGTSEAPGEYFFSDGVRLKKYRGMGSLEAMERGDAKGAAMSRYYHNEMDKMKVAQGVSGSIVDKGSVLRYLPYLECGLQHSCQDIGANSINKLRDMIYNGQLRFMKRTHSAQLEGNVHGLFSYEKRLF*HTTMAATETGSGGVPAPVQQQQQQQQHHQQQQHRHRSAAGPLLDYTRLFDRK

Target ORF information:

RefSeq Version	NM_001369990.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster raspberry (ras), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369990.1

ORF Insert Sequence:

ATGGAGAGCA CGACAAAGGT GAAGGTGAAC GGCTTTGTGG AATCGACGTC GTCTTCAGCG 
GCGCCGGCAA TCCAGACAAA GAGTACCACC GGATTCGATG CCGAGCTGCA GGATGGGCTG 
AGTTGTAAGG AACTGTTCCA GAACGGTGAG GGACTCACCT ACAACGACTT TCTCATACTG 
CCCGGCTACA TAGACTTCAC CGCCGAGGAG GTCGATCTCA GTTCGCCACT GACCAAGTCG 
CTGACATTGC GAGCACCGCT GGTTAGTTCG CCCATGGACA CGGTAACCGA ATCGGAGATG 
GCCATCGCCA TGGCGCTGTG TGGTGGCATT GGCATCATCC ATCACAACTG CACGCCGGAA 
TACCAGGCGT TGGAGGTGCA CAAGGTTAAG AAGTACAAGC ACGGCTTCAT GCGCGACCCC 
TCGGTGATGT CGCCCACGAA TACGGTGGGG GATGTGTTGG AGGCGCGGCG GAAGAACGGA 
TTCACCGGCT ATCCGGTCAC CGAGAACGGC AAACTTGGCG GCAAGCTGCT GGGCATGGTC 
ACGTCGCGAG ACATTGACTT CCGCGAGAAC CAGCCGGAGG TCCTGCTGGC CGACATCATG 
ACCACCGAAC TGGTCACCGC TCCCAATGGC ATCAATCTGC CCACGGCAAA CGCTATTCTC 
GAGAAGAGCA AGAAGGGCAA ACTGCCGATT GTGAATCAGG CCGGCGAACT GGTGGCCATG 
ATTGCCCGCA CGGATTTGAA GAAGGCCCGC TCCTACCCGA ATGCCTCCAA GGACTCCAAT 
AAGCAGCTCC TCGTCGGCGC TGCCATCGGA ACGCGATCGG AGGACAAGGC GCGCCTGGCT 
CTGCTGGTGG CCAACGGCGT GGACGTTATC ATCCTTGATT CATCGCAGGG CAACTCCGTC 
TACCAGGTGG AGATGATCAA GTACATTAAG GAGACCTATC CCGAACTACA GGTCATTGGT 
GGCAATGTGG TAACACGTGC CCAGGCCAAA AATCTCATTG ATGCCGGCGT CGATGGACTG 
CGTGTGGGCA TGGGTTCCGG TTCCATCTGC ATCACCCAGG AGGTGATGGC CTGCGGATGT 
CCTCAGGCCA CTGCTGTCTA CCAGGTGTCC ACGTACGCTC GCCAATTCGG AGTGCCAGTG 
ATCGCGGACG GAGGCATCCA GTCCATTGGA CACATTGTGA AGGCTATTGC ATTGGGAGCC 
AGCGCTGTAA TGATGGGCTC CCTGCTGGCG GGCACATCGG AGGCACCGGG CGAGTACTTC 
TTCTCCGACG GAGTGCGCCT TAAGAAGTAC CGCGGCATGG GCTCCCTGGA GGCCATGGAG 
CGCGGCGATG CCAAGGGCGC TGCCATGTCG CGCTACTACC ACAACGAGAT GGATAAAATG 
AAGGTGGCAC AGGGCGTCAG TGGCAGCATC GTGGACAAGG GCAGTGTGCT GCGCTATCTA 
CCCTATTTGG AGTGCGGACT GCAGCACAGT TGTCAGGACA TCGGTGCTAA CTCCATTAAT 
AAATTGAGGG ATATGATCTA TAATGGCCAA CTGCGCTTCA TGAAGCGCAC CCATTCCGCT 
CAACTGGAAG GCAATGTCCA TGGCCTCTTC AGCTACGAGA AGCGTCTCTT CTAACATACG 
ACGATGGCTG CGACGGAAAC GGGCAGTGGA GGAGTGCCTG CCCCTGTCCA GCAGCAGCAG 
CAGCAGCAGC AACACCATCA GCAGCAACAG CATCGCCACC GATCTGCAGC AGGCCCACTG 
CTCGACTACA CCCGCCTATT CGATCGCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ras cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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