eIF4G1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIF4G1
Entrez Gene ID	43839
Full Name	eukaryotic translation initiation factor 4G
Synonyms	CG10811,Dmel\CG10811,EIF4G,Eif4G,deIF4G,eIF-4G,eIF4g,p200
General protein information	Preferred Names eukaryotic translation initiation factor 4G Names CG10811 gene product from transcript CG10811-RA CG10811-PA CG10811-PB CG10811-PC Eukaryotic-initiation-factor-4G eIF-4G eIF4G-PA eIF4G-PB eIF4G-PC eukaryotic eukaryotic initiation factor 4G translation initiation factor translation initiation factor 4G translation initiation factor eIF4G
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102F1-102F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144385.2	NP_001137857.1	eukaryotic translation initiation factor 4G1, isoform C
NM_001103382.2	NP_001096852.1	eukaryotic translation initiation factor 4G1, isoform B
NM_079901.4	NP_524640.3	eukaryotic translation initiation factor 4G1, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The structures of eIF4E-eIF4G complexes reveal an extended interface to regulate translation initiation.
Title: The Structures of eIF4E-eIF4G Complexes Reveal an Extended Interface to Regulate Translation Initiation.

Phosphorylation of 4E-BP1 impairs the competition with eIF4G for eIF4E binding.
Title: Molecular architecture of 4E-BP translational inhibitors bound to eIF4E.

The eIF4G knockdown did not affect male fertility.
Title: Loss-of-function analysis reveals distinct requirements of the translation initiation factors eIF4E, eIF4E-3, eIF4G and eIF4G2 in Drosophila spermatogenesis.

Data show that in the early embryo dFMRP associates specifically with Caprin, and with eIF4G, a key regulator of translational initiation.
Title: dFMRP and Caprin, translational regulators of synaptic plasticity, control the cell cycle at the Drosophila mid-blastula transition.

A novel eIF4G homolog, Off-schedule, couples translational control to meiosis and differentiation in Drosophila spermatocytes.
Title: A novel eIF4G homolog, Off-schedule, couples translational control to meiosis and differentiation in Drosophila spermatocytes.

The following eIF4G1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF4G1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00199	NM_079901.4 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4G1 (eIF4G1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa00199	NM_001144385.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4G1 (eIF4G1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa00200	NM_001103382.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4G1 (eIF4G1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIF4G1 ( NM_001144385.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137857.1 Drosophila melanogaster eukaryotic translation initiation factor 4G1 (eIF4G1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00199
Clone ID Related Accession (Same CDS sequence)	NM_001144385.2 , NM_079901.4
Accession Version	NM_001144385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4G1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001144385.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACAGG CTATACCAAC TTTACCAACA CAATCAGATA TAGATAAAGC CATGCAGCCC 
CATTCAGCAC AAAATATGTA TGTATCAACG GGAAACAACA ATTCTGGGAA TACCCGTTCT 
AATCCACAAA GTGGCGGAAT ATTTCGAGGA CCACCCTCGA CGCCTAATGC CCCTAGAGGT 
GCTAGTGGCG GAGCAACACG GCATGTCCAT GTGCAACCCA TGTACTCACA ACCCCTTCAC 
CAGAACATGG TGCTACAACA ATACACACAA TATAACCCAC GGCAACAAAC ATTTCCAGCT 
TCTCATTTGC AATACGCACC CGCCCCCATG CCATACTACC AGTACCAATA TGTCCCAACT 
CTTCAGCAAC AGCCGCCGCA TACTCGTAGT GCTGTCACGG TAAATACGAA CGTCAATGTG 
GGAAACAATT TGCAGCCAGT GCAGTCTGGA CCCAATGGAC CTCTCCCTGT TCCGGGGGCT 
AGCTCGTCGC AAATTCAATT GCTCACAAGC ACAGTGCAGC CAGGAGCCAG CACTGTAATG 
GGCGTTGGCG GTCCTGGTAG TACAATGGGG CAAGTGGGTG TGCCACCAAT GGTTGGGGTT 
GGTGTAATGA CAACAAGTGT GCAACCGCAA CCTGTGCAGG TGCAGCCTGC TAGCCGGCGC 
CGGCATCAGC ATCGATTGCA GATAATCGAT CCGACGACTA AAAAAAATAT TTTAGACGAT 
TTTGATAAGA CAAAATCAAA CACAGACAAT GAATTTTCCG ATCAGGTAAC CTCCACAAAC 
ACACCAGCAA CTGTCCTTTC AGAAGGTCCA ATACGTATTC CACAGCAAGA AAGTGTTGGT 
TTAAATAATT TGACCAGTAC ATCATCACAA GGATCAGAAT CACGGACAAA CGCGCCTTAT 
ATCCCCATTG AAACAACACC AATAAGTCGA ACAGATGTTG GGCCGACTCC AATCGTATCC 
GCGATGACTG ATGCTCCTTC GGTTGAAATA TTACCAACGC CCCAAAGAGG GAGAAGCAAA 
AAAATTCCTA TAGTGTCTCC TAAAAATGTA TCTGAGTCCA TAGTTGCTCC TATCACTGAT 
GAAACGGATG ATGCAGGGTC AACACCGATA TCCAGAGCCA CTGAAGAATC TATGCCACCT 
AATCAGACCC ATCCAAATTT ACTCATATCC GATTCATCAA AACATAAGCA AGCAGTATCA 
AATAGTGAGA TTTCAAAAGA CGCTCCTACT GGTTTAAAGG AAATGCATGT TGCTGAGCTG 
GAGTCCAGTG TAGCCTCCGA AAATCATCCA GCTGGAATAC TAGATGTCAA TGTCAACAAG 
AGTTCACAAT CATTGGATTT TTCTGCAGAT CCCGAAATTG ATAGTATTGG CGCAATATCA 
CCTACGAGTC CAAACGCAGT TTCTCCTATT TTACACGAAG TTTTAGATAA TACTGAGTTG 
TCAAAAAAAT TAGAGAATTC TACGACAGAA AGATTTAAAG ATAGCCAAAG CGTAGAGAAG 
CCAACTCATC AAGAACTAAG TTTAAGAAAT GCCACAGACG AGACTGAAAT ATCCGCAATG 
GCATTACAAG ATGTGAATAG TTTGGATAAT AACCAAATTG AAAAAACATA CCCTTCAAAG 
CCGAAACTCA ATGTAGATGT TTCAGAAGAC ATTTCCTCCA GAGAGTCAGC AATAAAGTCA 
ACTTCTACAA AAAATACAGG AGTAGATGTA GGTCTCCAAT CAGATTCTAA GCCAGAGACT 
ACCCTTAACG ATAAACAGGA CTCTACTGAC CTAAAAGTGA AAGTGTCTGC GAAAATTTCA 
TCTATCATAA ACTATAATGA GGGTCAATGG TCACCAAACA ATCCAAGCGG AAAAAAACAA 
TACGACCGAG AACAGTTATT ACAGTTACGC GAGGTTAAGG CCTCACGTAT ACAACCAGAG 
GTAAAAAATG TGTCTATTCT TCCTCAACCA AACTTAATGC CATCATTTAT TCGAAATAAC 
AATAATAACA AGCGAGTACA ATCGATGGTT GGTATTATTG GCAATCGTAG CAATGAATCG 
GCGGGAAATT ATATTGGTAA ACAGATGTCG ATGTCTGGCG TACAAAGTGG CGGCGGTAGA 
AGCAGTATGA AGGGCATGAT ACACGTAAAT TTATCATTAA ATCAAGATGT AAAGTTAAGT 
GAAAATGAAA ATGCATGGCG CCCTCGGGTC TTAAATAAAT CTGATGGTGA TAGCGACGCG 
AAATCTGCAC TCGAAAAAGA TGAATTGGTA CGTCGTGTTC GGGGTATTTT AAATAAGCTT 
ACCCCAGAAA GGTTTGACAC ACTTGTTGAG GAGATTATAA AATTGAAAAT TGATACACCC 
GACAAAGTGG ACGAAGTAAT TGTGTTGGTA TTTGAAAAGG CCATTGATGA GCCAAACTTT 
TCTGTGTCTT ACGCCAGACT ATGTCAACGA CTTGCTGCGG AAGTGAAGGT AATAGACGAG 
CGCATGGAAA GCGAAACCAA ATCTAACTCG GCACACTTTC GAAATGCATT GTTAGATAAA 
ACTGAACAGG AATTCACCCA AAACGTTTCA CAAAGCACCG CAAAGGAAAA AAAATTACAG 
CCAATTGTGG ATAAAATTAA GAAATGTACT GATGCCAATG AAAAAGCGGA ACTTGAGGCA 
TTTTTGGAAG AAGAAGAAAG AAAAATAAGA AGACGATCCG GAGGGACAGT ACGATTTATT 
GGCGAACTTT TTAAGATATC TATGTTGACT GGCAAAATAA TATACTCTTG CATCGATACA 
CTTCTTAACC CTCATTCTGA AGACATGCTT GAGTGTTTGT GCAAGCTGCT TACAACTGTG 
GGTGCTAAAT TTGAAAAGAC CCCAGTAAAT TCCAAAGACC CTAGTCGGTG TTATTCGTTG 
GAAAAATCGA TCACCAGGAT GCAGGCCATC GCATCGAAAA CTGACAAAGA TGGAGCCAGA 
GTAAGCTCAA GAGTACGATT TATGCTTCAA GACGTTATCG ACTTGCGTAA GAATAAATGG 
CAAACTTCGC GGAATGAAGC ACCCAAAACT ATGGGACAAA TTGAAAAAGA GGCAAAGAAT 
GAGCAGCTTT CTGCACAATA TTTTGGTACA CTTTCCAGTA CTACACCCGG AGGATCGCAG 
GGCGGCTCTG GTAAACGAGA TGACCGTGGA AACTCTCGAT ACGGTGAATC TCGTTCAAGT 
AGCGCGTATG GTGGCAGCCA CAGTCAACGT GGTGATAATG GCAACCTTCG ACACCAGCAG 
CAGAACAACG TCGGGGGAAA CGTCTCTGGA GGTGCTGGAC ACTCCAATGG AAACAATGAT 
GAAAATACTT GGCATGTGCA AACCAGCAAA GGTAGCCGCT CTCTAGCGGT TGACAGCAAT 
AAACTGGAAG GCCTGTCTAA ACTTTCTGAC CAAAATCTGG AAACAAAGAA GATGGGTGGA 
TTAACGCAAT TCATTTGGAT TAGTAGTGAC ACGACAAGAC TATCGTCGGC GCCGACACCA 
ACCCCATCCA ATCCTTTCGC TGTGCTCTCA TCCCTAATTG ATAAAAATAG TAATGAAAGG 
GATCGCGATC GCAGTGGACC CAGAAACAAA GGATCATACA ACAAAGGTTC TATGGAGCGG 
GATCGTTATG ATAGAGGTAT GCATTCGAGA ACTGGATCAT CTCAGGGATC ACGAGAAAAC 
TCATCCTCCC GCGGTGGTCA GCAGGGTCGA ACTTTATTGA GTTCATCAGT TCAAAAATCT 
ACTAGTCATT CAAAATACAC CCAACAGGCA CCACCTACAC GGCATACTGT TAAAGCCCAG 
TCATCAGTGG GCAGCTCTAA CGTTAACACA GGTCCACTCT ACCGTGGAAG TGAGCAGCAA 
ACATCAGCAA CCTTCTCGCA GACCACCCGT TCTGTTGCTC CTGTAGCAGT GTTCATCGAA 
GCTAGCGAAA CAGATTTAAA ACTCATCAAA TCTGTTGTTT CAGAAATTGT AGACCTATCA 
GCTGCATCTA AAGAAGTCAC GCCCGGTGCA GTTTCGTGCA TAAAACGAGT ACCAGAGAAA 
CTACGGTGCA GTTTCATTTA TTATATTTTA ACAGATTATT TGCATTTAGC AAATGTAGGT 
AAACAGTACA GGCGGTATTT ATCTATTGCC GTCTCCCAAC TTATTCAGCA GAATTACATT 
TCTGCCGATC ATTTGAGACT GGCTTACAAC GAATTTACCG TATATGCAAA CGATTTGATT 
GTTGATATTC CGGAGCTTTG GCTATACATT CTTCAATTTG CCGGGCCACT AATTGTAAAG 
AAAATATTGA CTATATCAGA TTTGTGGAAC AACAATTTAA AAGAAAATAG TCCATCAAAC 
GTCGCTAAAA AGTTCCTTAA AACATACTTG ATATATTGCA CACAAGAAGT TGGACCAAAT 
TTTGCTCGGA ATATGTGGAT CAAGTTTAAC CTAAAATGGT CAGATTTTAT GCCTGAAAGT 
GAAGTTGCCG ACTTTATTAA ATTTAACAGA CTGGAATATG TGGAAAACGA ATCAAAGTCG 
CCAGTGATTG ATCATAGAGA GACTCCAGAA AAGCACGTTA AAAATGTGAT AGACCATATT 
GAGCACTTAC TAAAGGAAGG AACAACGGCG GACTGCATTA TTGATTATAG TAACGGAAAT 
ATAATGGTGG TTGATAAGTT GTTTATCAGA GGATTGACGG AAACATTGAG TAATTTCTCG 
ATTCACTACA AGGACAACAG TTACAAGCTT GAGTCCGAAA CCTTTCAAAA GTTTTGCATA 
CCGGTTTTGC AGAGATACAT TGATTCTAAT GAAGACCACC AACTTGAATG TTTATATACT 
TTGCAGCTTT TAGTTCATGG ATTGGAGCAT CCAAGAGGGT TGCTGAGTGA ACTAATTGGC 
GAGCTATATG ATGCCTTTGT AATACAGAAG GAATCGCTTT GTAAGTGGCG AGATTCAAAG 
GATCAGTCTG CTGGCAAGGG CGTTGCTGTA AAAAGTCTTA ATCCTTTTTT TAACTCGTTG 
TTAAACGATG ATGCCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137857.1
CDS	611..5611
Translation	MQQAIPTLPTQSDIDKAMQPHSAQNMYVSTGNNNSGNTRSNPQSGGIFRGPPSTPNAPRGASGGATRHVHVQPMYSQPLHQNMVLQQYTQYNPRQQTFPASHLQYAPAPMPYYQYQYVPTLQQQPPHTRSAVTVNTNVNVGNNLQPVQSGPNGPLPVPGASSSQIQLLTSTVQPGASTVMGVGGPGSTMGQVGVPPMVGVGVMTTSVQPQPVQVQPASRRRHQHRLQIIDPTTKKNILDDFDKTKSNTDNEFSDQVTSTNTPATVLSEGPIRIPQQESVGLNNLTSTSSQGSESRTNAPYIPIETTPISRTDVGPTPIVSAMTDAPSVEILPTPQRGRSKKIPIVSPKNVSESIVAPITDETDDAGSTPISRATEESMPPNQTHPNLLISDSSKHKQAVSNSEISKDAPTGLKEMHVAELESSVASENHPAGILDVNVNKSSQSLDFSADPEIDSIGAISPTSPNAVSPILHEVLDNTELSKKLENSTTERFKDSQSVEKPTHQELSLRNATDETEISAMALQDVNSLDNNQIEKTYPSKPKLNVDVSEDISSRESAIKSTSTKNTGVDVGLQSDSKPETTLNDKQDSTDLKVKVSAKISSIINYNEGQWSPNNPSGKKQYDREQLLQLREVKASRIQPEVKNVSILPQPNLMPSFIRNNNNNKRVQSMVGIIGNRSNESAGNYIGKQMSMSGVQSGGGRSSMKGMIHVNLSLNQDVKLSENENAWRPRVLNKSDGDSDAKSALEKDELVRRVRGILNKLTPERFDTLVEEIIKLKIDTPDKVDEVIVLVFEKAIDEPNFSVSYARLCQRLAAEVKVIDERMESETKSNSAHFRNALLDKTEQEFTQNVSQSTAKEKKLQPIVDKIKKCTDANEKAELEAFLEEEERKIRRRSGGTVRFIGELFKISMLTGKIIYSCIDTLLNPHSEDMLECLCKLLTTVGAKFEKTPVNSKDPSRCYSLEKSITRMQAIASKTDKDGARVSSRVRFMLQDVIDLRKNKWQTSRNEAPKTMGQIEKEAKNEQLSAQYFGTLSSTTPGGSQGGSGKRDDRGNSRYGESRSSSAYGGSHSQRGDNGNLRHQQQNNVGGNVSGGAGHSNGNNDENTWHVQTSKGSRSLAVDSNKLEGLSKLSDQNLETKKMGGLTQFIWISSDTTRLSSAPTPTPSNPFAVLSSLIDKNSNERDRDRSGPRNKGSYNKGSMERDRYDRGMHSRTGSSQGSRENSSSRGGQQGRTLLSSSVQKSTSHSKYTQQAPPTRHTVKAQSSVGSSNVNTGPLYRGSEQQTSATFSQTTRSVAPVAVFIEASETDLKLIKSVVSEIVDLSAASKEVTPGAVSCIKRVPEKLRCSFIYYILTDYLHLANVGKQYRRYLSIAVSQLIQQNYISADHLRLAYNEFTVYANDLIVDIPELWLYILQFAGPLIVKKILTISDLWNNNLKENSPSNVAKKFLKTYLIYCTQEVGPNFARNMWIKFNLKWSDFMPESEVADFIKFNRLEYVENESKSPVIDHRETPEKHVKNVIDHIEHLLKEGTTADCIIDYSNGNIMVVDKLFIRGLTETLSNFSIHYKDNSYKLESETFQKFCIPVLQRYIDSNEDHQLECLYTLQLLVHGLEHPRGLLSELIGELYDAFVIQKESLCKWRDSKDQSAGKGVAVKSLNPFFNSLLNDDAN

Target ORF information:

RefSeq Version	NM_001144385.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4G1 (eIF4G1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144385.2

ORF Insert Sequence:

ATGCAACAGG CTATACCAAC TTTACCAACA CAATCAGATA TAGATAAAGC CATGCAGCCC 
CATTCAGCAC AAAATATGTA TGTATCAACG GGAAACAACA ATTCTGGGAA TACCCGTTCT 
AATCCACAAA GTGGCGGAAT ATTTCGAGGA CCACCCTCGA CGCCTAATGC CCCTAGAGGT 
GCTAGTGGCG GAGCAACACG GCATGTCCAT GTGCAACCCA TGTACTCACA ACCCCTTCAC 
CAGAACATGG TGCTACAACA ATACACACAA TATAACCCAC GGCAACAAAC ATTTCCAGCT 
TCTCATTTGC AATACGCACC CGCCCCCATG CCATACTACC AGTACCAATA TGTCCCAACT 
CTTCAGCAAC AGCCGCCGCA TACTCGTAGT GCTGTCACGG TAAATACGAA CGTCAATGTG 
GGAAACAATT TGCAGCCAGT GCAGTCTGGA CCCAATGGAC CTCTCCCTGT TCCGGGGGCT 
AGCTCGTCGC AAATTCAATT GCTCACAAGC ACAGTGCAGC CAGGAGCCAG CACTGTAATG 
GGCGTTGGCG GTCCTGGTAG TACAATGGGG CAAGTGGGTG TGCCACCAAT GGTTGGGGTT 
GGTGTAATGA CAACAAGTGT GCAACCGCAA CCTGTGCAGG TGCAGCCTGC TAGCCGGCGC 
CGGCATCAGC ATCGATTGCA GATAATCGAT CCGACGACTA AAAAAAATAT TTTAGACGAT 
TTTGATAAGA CAAAATCAAA CACAGACAAT GAATTTTCCG ATCAGGTAAC CTCCACAAAC 
ACACCAGCAA CTGTCCTTTC AGAAGGTCCA ATACGTATTC CACAGCAAGA AAGTGTTGGT 
TTAAATAATT TGACCAGTAC ATCATCACAA GGATCAGAAT CACGGACAAA CGCGCCTTAT 
ATCCCCATTG AAACAACACC AATAAGTCGA ACAGATGTTG GGCCGACTCC AATCGTATCC 
GCGATGACTG ATGCTCCTTC GGTTGAAATA TTACCAACGC CCCAAAGAGG GAGAAGCAAA 
AAAATTCCTA TAGTGTCTCC TAAAAATGTA TCTGAGTCCA TAGTTGCTCC TATCACTGAT 
GAAACGGATG ATGCAGGGTC AACACCGATA TCCAGAGCCA CTGAAGAATC TATGCCACCT 
AATCAGACCC ATCCAAATTT ACTCATATCC GATTCATCAA AACATAAGCA AGCAGTATCA 
AATAGTGAGA TTTCAAAAGA CGCTCCTACT GGTTTAAAGG AAATGCATGT TGCTGAGCTG 
GAGTCCAGTG TAGCCTCCGA AAATCATCCA GCTGGAATAC TAGATGTCAA TGTCAACAAG 
AGTTCACAAT CATTGGATTT TTCTGCAGAT CCCGAAATTG ATAGTATTGG CGCAATATCA 
CCTACGAGTC CAAACGCAGT TTCTCCTATT TTACACGAAG TTTTAGATAA TACTGAGTTG 
TCAAAAAAAT TAGAGAATTC TACGACAGAA AGATTTAAAG ATAGCCAAAG CGTAGAGAAG 
CCAACTCATC AAGAACTAAG TTTAAGAAAT GCCACAGACG AGACTGAAAT ATCCGCAATG 
GCATTACAAG ATGTGAATAG TTTGGATAAT AACCAAATTG AAAAAACATA CCCTTCAAAG 
CCGAAACTCA ATGTAGATGT TTCAGAAGAC ATTTCCTCCA GAGAGTCAGC AATAAAGTCA 
ACTTCTACAA AAAATACAGG AGTAGATGTA GGTCTCCAAT CAGATTCTAA GCCAGAGACT 
ACCCTTAACG ATAAACAGGA CTCTACTGAC CTAAAAGTGA AAGTGTCTGC GAAAATTTCA 
TCTATCATAA ACTATAATGA GGGTCAATGG TCACCAAACA ATCCAAGCGG AAAAAAACAA 
TACGACCGAG AACAGTTATT ACAGTTACGC GAGGTTAAGG CCTCACGTAT ACAACCAGAG 
GTAAAAAATG TGTCTATTCT TCCTCAACCA AACTTAATGC CATCATTTAT TCGAAATAAC 
AATAATAACA AGCGAGTACA ATCGATGGTT GGTATTATTG GCAATCGTAG CAATGAATCG 
GCGGGAAATT ATATTGGTAA ACAGATGTCG ATGTCTGGCG TACAAAGTGG CGGCGGTAGA 
AGCAGTATGA AGGGCATGAT ACACGTAAAT TTATCATTAA ATCAAGATGT AAAGTTAAGT 
GAAAATGAAA ATGCATGGCG CCCTCGGGTC TTAAATAAAT CTGATGGTGA TAGCGACGCG 
AAATCTGCAC TCGAAAAAGA TGAATTGGTA CGTCGTGTTC GGGGTATTTT AAATAAGCTT 
ACCCCAGAAA GGTTTGACAC ACTTGTTGAG GAGATTATAA AATTGAAAAT TGATACACCC 
GACAAAGTGG ACGAAGTAAT TGTGTTGGTA TTTGAAAAGG CCATTGATGA GCCAAACTTT 
TCTGTGTCTT ACGCCAGACT ATGTCAACGA CTTGCTGCGG AAGTGAAGGT AATAGACGAG 
CGCATGGAAA GCGAAACCAA ATCTAACTCG GCACACTTTC GAAATGCATT GTTAGATAAA 
ACTGAACAGG AATTCACCCA AAACGTTTCA CAAAGCACCG CAAAGGAAAA AAAATTACAG 
CCAATTGTGG ATAAAATTAA GAAATGTACT GATGCCAATG AAAAAGCGGA ACTTGAGGCA 
TTTTTGGAAG AAGAAGAAAG AAAAATAAGA AGACGATCCG GAGGGACAGT ACGATTTATT 
GGCGAACTTT TTAAGATATC TATGTTGACT GGCAAAATAA TATACTCTTG CATCGATACA 
CTTCTTAACC CTCATTCTGA AGACATGCTT GAGTGTTTGT GCAAGCTGCT TACAACTGTG 
GGTGCTAAAT TTGAAAAGAC CCCAGTAAAT TCCAAAGACC CTAGTCGGTG TTATTCGTTG 
GAAAAATCGA TCACCAGGAT GCAGGCCATC GCATCGAAAA CTGACAAAGA TGGAGCCAGA 
GTAAGCTCAA GAGTACGATT TATGCTTCAA GACGTTATCG ACTTGCGTAA GAATAAATGG 
CAAACTTCGC GGAATGAAGC ACCCAAAACT ATGGGACAAA TTGAAAAAGA GGCAAAGAAT 
GAGCAGCTTT CTGCACAATA TTTTGGTACA CTTTCCAGTA CTACACCCGG AGGATCGCAG 
GGCGGCTCTG GTAAACGAGA TGACCGTGGA AACTCTCGAT ACGGTGAATC TCGTTCAAGT 
AGCGCGTATG GTGGCAGCCA CAGTCAACGT GGTGATAATG GCAACCTTCG ACACCAGCAG 
CAGAACAACG TCGGGGGAAA CGTCTCTGGA GGTGCTGGAC ACTCCAATGG AAACAATGAT 
GAAAATACTT GGCATGTGCA AACCAGCAAA GGTAGCCGCT CTCTAGCGGT TGACAGCAAT 
AAACTGGAAG GCCTGTCTAA ACTTTCTGAC CAAAATCTGG AAACAAAGAA GATGGGTGGA 
TTAACGCAAT TCATTTGGAT TAGTAGTGAC ACGACAAGAC TATCGTCGGC GCCGACACCA 
ACCCCATCCA ATCCTTTCGC TGTGCTCTCA TCCCTAATTG ATAAAAATAG TAATGAAAGG 
GATCGCGATC GCAGTGGACC CAGAAACAAA GGATCATACA ACAAAGGTTC TATGGAGCGG 
GATCGTTATG ATAGAGGTAT GCATTCGAGA ACTGGATCAT CTCAGGGATC ACGAGAAAAC 
TCATCCTCCC GCGGTGGTCA GCAGGGTCGA ACTTTATTGA GTTCATCAGT TCAAAAATCT 
ACTAGTCATT CAAAATACAC CCAACAGGCA CCACCTACAC GGCATACTGT TAAAGCCCAG 
TCATCAGTGG GCAGCTCTAA CGTTAACACA GGTCCACTCT ACCGTGGAAG TGAGCAGCAA 
ACATCAGCAA CCTTCTCGCA GACCACCCGT TCTGTTGCTC CTGTAGCAGT GTTCATCGAA 
GCTAGCGAAA CAGATTTAAA ACTCATCAAA TCTGTTGTTT CAGAAATTGT AGACCTATCA 
GCTGCATCTA AAGAAGTCAC GCCCGGTGCA GTTTCGTGCA TAAAACGAGT ACCAGAGAAA 
CTACGGTGCA GTTTCATTTA TTATATTTTA ACAGATTATT TGCATTTAGC AAATGTAGGT 
AAACAGTACA GGCGGTATTT ATCTATTGCC GTCTCCCAAC TTATTCAGCA GAATTACATT 
TCTGCCGATC ATTTGAGACT GGCTTACAAC GAATTTACCG TATATGCAAA CGATTTGATT 
GTTGATATTC CGGAGCTTTG GCTATACATT CTTCAATTTG CCGGGCCACT AATTGTAAAG 
AAAATATTGA CTATATCAGA TTTGTGGAAC AACAATTTAA AAGAAAATAG TCCATCAAAC 
GTCGCTAAAA AGTTCCTTAA AACATACTTG ATATATTGCA CACAAGAAGT TGGACCAAAT 
TTTGCTCGGA ATATGTGGAT CAAGTTTAAC CTAAAATGGT CAGATTTTAT GCCTGAAAGT 
GAAGTTGCCG ACTTTATTAA ATTTAACAGA CTGGAATATG TGGAAAACGA ATCAAAGTCG 
CCAGTGATTG ATCATAGAGA GACTCCAGAA AAGCACGTTA AAAATGTGAT AGACCATATT 
GAGCACTTAC TAAAGGAAGG AACAACGGCG GACTGCATTA TTGATTATAG TAACGGAAAT 
ATAATGGTGG TTGATAAGTT GTTTATCAGA GGATTGACGG AAACATTGAG TAATTTCTCG 
ATTCACTACA AGGACAACAG TTACAAGCTT GAGTCCGAAA CCTTTCAAAA GTTTTGCATA 
CCGGTTTTGC AGAGATACAT TGATTCTAAT GAAGACCACC AACTTGAATG TTTATATACT 
TTGCAGCTTT TAGTTCATGG ATTGGAGCAT CCAAGAGGGT TGCTGAGTGA ACTAATTGGC 
GAGCTATATG ATGCCTTTGT AATACAGAAG GAATCGCTTT GTAAGTGGCG AGATTCAAAG 
GATCAGTCTG CTGGCAAGGG CGTTGCTGTA AAAAGTCTTA ATCCTTTTTT TAACTCGTTG 
TTAAACGATG ATGCCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4G1 ( NM_079901.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524640.3 Drosophila melanogaster eukaryotic translation initiation factor 4G1 (eIF4G1), transcript variant A, mRNA.

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CloneID	OFa00199
Clone ID Related Accession (Same CDS sequence)	NM_001144385.2 , NM_079901.4
Accession Version	NM_079901.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4G1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_079901.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACAGG CTATACCAAC TTTACCAACA CAATCAGATA TAGATAAAGC CATGCAGCCC 
CATTCAGCAC AAAATATGTA TGTATCAACG GGAAACAACA ATTCTGGGAA TACCCGTTCT 
AATCCACAAA GTGGCGGAAT ATTTCGAGGA CCACCCTCGA CGCCTAATGC CCCTAGAGGT 
GCTAGTGGCG GAGCAACACG GCATGTCCAT GTGCAACCCA TGTACTCACA ACCCCTTCAC 
CAGAACATGG TGCTACAACA ATACACACAA TATAACCCAC GGCAACAAAC ATTTCCAGCT 
TCTCATTTGC AATACGCACC CGCCCCCATG CCATACTACC AGTACCAATA TGTCCCAACT 
CTTCAGCAAC AGCCGCCGCA TACTCGTAGT GCTGTCACGG TAAATACGAA CGTCAATGTG 
GGAAACAATT TGCAGCCAGT GCAGTCTGGA CCCAATGGAC CTCTCCCTGT TCCGGGGGCT 
AGCTCGTCGC AAATTCAATT GCTCACAAGC ACAGTGCAGC CAGGAGCCAG CACTGTAATG 
GGCGTTGGCG GTCCTGGTAG TACAATGGGG CAAGTGGGTG TGCCACCAAT GGTTGGGGTT 
GGTGTAATGA CAACAAGTGT GCAACCGCAA CCTGTGCAGG TGCAGCCTGC TAGCCGGCGC 
CGGCATCAGC ATCGATTGCA GATAATCGAT CCGACGACTA AAAAAAATAT TTTAGACGAT 
TTTGATAAGA CAAAATCAAA CACAGACAAT GAATTTTCCG ATCAGGTAAC CTCCACAAAC 
ACACCAGCAA CTGTCCTTTC AGAAGGTCCA ATACGTATTC CACAGCAAGA AAGTGTTGGT 
TTAAATAATT TGACCAGTAC ATCATCACAA GGATCAGAAT CACGGACAAA CGCGCCTTAT 
ATCCCCATTG AAACAACACC AATAAGTCGA ACAGATGTTG GGCCGACTCC AATCGTATCC 
GCGATGACTG ATGCTCCTTC GGTTGAAATA TTACCAACGC CCCAAAGAGG GAGAAGCAAA 
AAAATTCCTA TAGTGTCTCC TAAAAATGTA TCTGAGTCCA TAGTTGCTCC TATCACTGAT 
GAAACGGATG ATGCAGGGTC AACACCGATA TCCAGAGCCA CTGAAGAATC TATGCCACCT 
AATCAGACCC ATCCAAATTT ACTCATATCC GATTCATCAA AACATAAGCA AGCAGTATCA 
AATAGTGAGA TTTCAAAAGA CGCTCCTACT GGTTTAAAGG AAATGCATGT TGCTGAGCTG 
GAGTCCAGTG TAGCCTCCGA AAATCATCCA GCTGGAATAC TAGATGTCAA TGTCAACAAG 
AGTTCACAAT CATTGGATTT TTCTGCAGAT CCCGAAATTG ATAGTATTGG CGCAATATCA 
CCTACGAGTC CAAACGCAGT TTCTCCTATT TTACACGAAG TTTTAGATAA TACTGAGTTG 
TCAAAAAAAT TAGAGAATTC TACGACAGAA AGATTTAAAG ATAGCCAAAG CGTAGAGAAG 
CCAACTCATC AAGAACTAAG TTTAAGAAAT GCCACAGACG AGACTGAAAT ATCCGCAATG 
GCATTACAAG ATGTGAATAG TTTGGATAAT AACCAAATTG AAAAAACATA CCCTTCAAAG 
CCGAAACTCA ATGTAGATGT TTCAGAAGAC ATTTCCTCCA GAGAGTCAGC AATAAAGTCA 
ACTTCTACAA AAAATACAGG AGTAGATGTA GGTCTCCAAT CAGATTCTAA GCCAGAGACT 
ACCCTTAACG ATAAACAGGA CTCTACTGAC CTAAAAGTGA AAGTGTCTGC GAAAATTTCA 
TCTATCATAA ACTATAATGA GGGTCAATGG TCACCAAACA ATCCAAGCGG AAAAAAACAA 
TACGACCGAG AACAGTTATT ACAGTTACGC GAGGTTAAGG CCTCACGTAT ACAACCAGAG 
GTAAAAAATG TGTCTATTCT TCCTCAACCA AACTTAATGC CATCATTTAT TCGAAATAAC 
AATAATAACA AGCGAGTACA ATCGATGGTT GGTATTATTG GCAATCGTAG CAATGAATCG 
GCGGGAAATT ATATTGGTAA ACAGATGTCG ATGTCTGGCG TACAAAGTGG CGGCGGTAGA 
AGCAGTATGA AGGGCATGAT ACACGTAAAT TTATCATTAA ATCAAGATGT AAAGTTAAGT 
GAAAATGAAA ATGCATGGCG CCCTCGGGTC TTAAATAAAT CTGATGGTGA TAGCGACGCG 
AAATCTGCAC TCGAAAAAGA TGAATTGGTA CGTCGTGTTC GGGGTATTTT AAATAAGCTT 
ACCCCAGAAA GGTTTGACAC ACTTGTTGAG GAGATTATAA AATTGAAAAT TGATACACCC 
GACAAAGTGG ACGAAGTAAT TGTGTTGGTA TTTGAAAAGG CCATTGATGA GCCAAACTTT 
TCTGTGTCTT ACGCCAGACT ATGTCAACGA CTTGCTGCGG AAGTGAAGGT AATAGACGAG 
CGCATGGAAA GCGAAACCAA ATCTAACTCG GCACACTTTC GAAATGCATT GTTAGATAAA 
ACTGAACAGG AATTCACCCA AAACGTTTCA CAAAGCACCG CAAAGGAAAA AAAATTACAG 
CCAATTGTGG ATAAAATTAA GAAATGTACT GATGCCAATG AAAAAGCGGA ACTTGAGGCA 
TTTTTGGAAG AAGAAGAAAG AAAAATAAGA AGACGATCCG GAGGGACAGT ACGATTTATT 
GGCGAACTTT TTAAGATATC TATGTTGACT GGCAAAATAA TATACTCTTG CATCGATACA 
CTTCTTAACC CTCATTCTGA AGACATGCTT GAGTGTTTGT GCAAGCTGCT TACAACTGTG 
GGTGCTAAAT TTGAAAAGAC CCCAGTAAAT TCCAAAGACC CTAGTCGGTG TTATTCGTTG 
GAAAAATCGA TCACCAGGAT GCAGGCCATC GCATCGAAAA CTGACAAAGA TGGAGCCAGA 
GTAAGCTCAA GAGTACGATT TATGCTTCAA GACGTTATCG ACTTGCGTAA GAATAAATGG 
CAAACTTCGC GGAATGAAGC ACCCAAAACT ATGGGACAAA TTGAAAAAGA GGCAAAGAAT 
GAGCAGCTTT CTGCACAATA TTTTGGTACA CTTTCCAGTA CTACACCCGG AGGATCGCAG 
GGCGGCTCTG GTAAACGAGA TGACCGTGGA AACTCTCGAT ACGGTGAATC TCGTTCAAGT 
AGCGCGTATG GTGGCAGCCA CAGTCAACGT GGTGATAATG GCAACCTTCG ACACCAGCAG 
CAGAACAACG TCGGGGGAAA CGTCTCTGGA GGTGCTGGAC ACTCCAATGG AAACAATGAT 
GAAAATACTT GGCATGTGCA AACCAGCAAA GGTAGCCGCT CTCTAGCGGT TGACAGCAAT 
AAACTGGAAG GCCTGTCTAA ACTTTCTGAC CAAAATCTGG AAACAAAGAA GATGGGTGGA 
TTAACGCAAT TCATTTGGAT TAGTAGTGAC ACGACAAGAC TATCGTCGGC GCCGACACCA 
ACCCCATCCA ATCCTTTCGC TGTGCTCTCA TCCCTAATTG ATAAAAATAG TAATGAAAGG 
GATCGCGATC GCAGTGGACC CAGAAACAAA GGATCATACA ACAAAGGTTC TATGGAGCGG 
GATCGTTATG ATAGAGGTAT GCATTCGAGA ACTGGATCAT CTCAGGGATC ACGAGAAAAC 
TCATCCTCCC GCGGTGGTCA GCAGGGTCGA ACTTTATTGA GTTCATCAGT TCAAAAATCT 
ACTAGTCATT CAAAATACAC CCAACAGGCA CCACCTACAC GGCATACTGT TAAAGCCCAG 
TCATCAGTGG GCAGCTCTAA CGTTAACACA GGTCCACTCT ACCGTGGAAG TGAGCAGCAA 
ACATCAGCAA CCTTCTCGCA GACCACCCGT TCTGTTGCTC CTGTAGCAGT GTTCATCGAA 
GCTAGCGAAA CAGATTTAAA ACTCATCAAA TCTGTTGTTT CAGAAATTGT AGACCTATCA 
GCTGCATCTA AAGAAGTCAC GCCCGGTGCA GTTTCGTGCA TAAAACGAGT ACCAGAGAAA 
CTACGGTGCA GTTTCATTTA TTATATTTTA ACAGATTATT TGCATTTAGC AAATGTAGGT 
AAACAGTACA GGCGGTATTT ATCTATTGCC GTCTCCCAAC TTATTCAGCA GAATTACATT 
TCTGCCGATC ATTTGAGACT GGCTTACAAC GAATTTACCG TATATGCAAA CGATTTGATT 
GTTGATATTC CGGAGCTTTG GCTATACATT CTTCAATTTG CCGGGCCACT AATTGTAAAG 
AAAATATTGA CTATATCAGA TTTGTGGAAC AACAATTTAA AAGAAAATAG TCCATCAAAC 
GTCGCTAAAA AGTTCCTTAA AACATACTTG ATATATTGCA CACAAGAAGT TGGACCAAAT 
TTTGCTCGGA ATATGTGGAT CAAGTTTAAC CTAAAATGGT CAGATTTTAT GCCTGAAAGT 
GAAGTTGCCG ACTTTATTAA ATTTAACAGA CTGGAATATG TGGAAAACGA ATCAAAGTCG 
CCAGTGATTG ATCATAGAGA GACTCCAGAA AAGCACGTTA AAAATGTGAT AGACCATATT 
GAGCACTTAC TAAAGGAAGG AACAACGGCG GACTGCATTA TTGATTATAG TAACGGAAAT 
ATAATGGTGG TTGATAAGTT GTTTATCAGA GGATTGACGG AAACATTGAG TAATTTCTCG 
ATTCACTACA AGGACAACAG TTACAAGCTT GAGTCCGAAA CCTTTCAAAA GTTTTGCATA 
CCGGTTTTGC AGAGATACAT TGATTCTAAT GAAGACCACC AACTTGAATG TTTATATACT 
TTGCAGCTTT TAGTTCATGG ATTGGAGCAT CCAAGAGGGT TGCTGAGTGA ACTAATTGGC 
GAGCTATATG ATGCCTTTGT AATACAGAAG GAATCGCTTT GTAAGTGGCG AGATTCAAAG 
GATCAGTCTG CTGGCAAGGG CGTTGCTGTA AAAAGTCTTA ATCCTTTTTT TAACTCGTTG 
TTAAACGATG ATGCCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524640.3
CDS	381..5381
Translation	MQQAIPTLPTQSDIDKAMQPHSAQNMYVSTGNNNSGNTRSNPQSGGIFRGPPSTPNAPRGASGGATRHVHVQPMYSQPLHQNMVLQQYTQYNPRQQTFPASHLQYAPAPMPYYQYQYVPTLQQQPPHTRSAVTVNTNVNVGNNLQPVQSGPNGPLPVPGASSSQIQLLTSTVQPGASTVMGVGGPGSTMGQVGVPPMVGVGVMTTSVQPQPVQVQPASRRRHQHRLQIIDPTTKKNILDDFDKTKSNTDNEFSDQVTSTNTPATVLSEGPIRIPQQESVGLNNLTSTSSQGSESRTNAPYIPIETTPISRTDVGPTPIVSAMTDAPSVEILPTPQRGRSKKIPIVSPKNVSESIVAPITDETDDAGSTPISRATEESMPPNQTHPNLLISDSSKHKQAVSNSEISKDAPTGLKEMHVAELESSVASENHPAGILDVNVNKSSQSLDFSADPEIDSIGAISPTSPNAVSPILHEVLDNTELSKKLENSTTERFKDSQSVEKPTHQELSLRNATDETEISAMALQDVNSLDNNQIEKTYPSKPKLNVDVSEDISSRESAIKSTSTKNTGVDVGLQSDSKPETTLNDKQDSTDLKVKVSAKISSIINYNEGQWSPNNPSGKKQYDREQLLQLREVKASRIQPEVKNVSILPQPNLMPSFIRNNNNNKRVQSMVGIIGNRSNESAGNYIGKQMSMSGVQSGGGRSSMKGMIHVNLSLNQDVKLSENENAWRPRVLNKSDGDSDAKSALEKDELVRRVRGILNKLTPERFDTLVEEIIKLKIDTPDKVDEVIVLVFEKAIDEPNFSVSYARLCQRLAAEVKVIDERMESETKSNSAHFRNALLDKTEQEFTQNVSQSTAKEKKLQPIVDKIKKCTDANEKAELEAFLEEEERKIRRRSGGTVRFIGELFKISMLTGKIIYSCIDTLLNPHSEDMLECLCKLLTTVGAKFEKTPVNSKDPSRCYSLEKSITRMQAIASKTDKDGARVSSRVRFMLQDVIDLRKNKWQTSRNEAPKTMGQIEKEAKNEQLSAQYFGTLSSTTPGGSQGGSGKRDDRGNSRYGESRSSSAYGGSHSQRGDNGNLRHQQQNNVGGNVSGGAGHSNGNNDENTWHVQTSKGSRSLAVDSNKLEGLSKLSDQNLETKKMGGLTQFIWISSDTTRLSSAPTPTPSNPFAVLSSLIDKNSNERDRDRSGPRNKGSYNKGSMERDRYDRGMHSRTGSSQGSRENSSSRGGQQGRTLLSSSVQKSTSHSKYTQQAPPTRHTVKAQSSVGSSNVNTGPLYRGSEQQTSATFSQTTRSVAPVAVFIEASETDLKLIKSVVSEIVDLSAASKEVTPGAVSCIKRVPEKLRCSFIYYILTDYLHLANVGKQYRRYLSIAVSQLIQQNYISADHLRLAYNEFTVYANDLIVDIPELWLYILQFAGPLIVKKILTISDLWNNNLKENSPSNVAKKFLKTYLIYCTQEVGPNFARNMWIKFNLKWSDFMPESEVADFIKFNRLEYVENESKSPVIDHRETPEKHVKNVIDHIEHLLKEGTTADCIIDYSNGNIMVVDKLFIRGLTETLSNFSIHYKDNSYKLESETFQKFCIPVLQRYIDSNEDHQLECLYTLQLLVHGLEHPRGLLSELIGELYDAFVIQKESLCKWRDSKDQSAGKGVAVKSLNPFFNSLLNDDAN

Target ORF information:

RefSeq Version	NM_079901.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4G1 (eIF4G1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079901.4

ORF Insert Sequence:

ATGCAACAGG CTATACCAAC TTTACCAACA CAATCAGATA TAGATAAAGC CATGCAGCCC 
CATTCAGCAC AAAATATGTA TGTATCAACG GGAAACAACA ATTCTGGGAA TACCCGTTCT 
AATCCACAAA GTGGCGGAAT ATTTCGAGGA CCACCCTCGA CGCCTAATGC CCCTAGAGGT 
GCTAGTGGCG GAGCAACACG GCATGTCCAT GTGCAACCCA TGTACTCACA ACCCCTTCAC 
CAGAACATGG TGCTACAACA ATACACACAA TATAACCCAC GGCAACAAAC ATTTCCAGCT 
TCTCATTTGC AATACGCACC CGCCCCCATG CCATACTACC AGTACCAATA TGTCCCAACT 
CTTCAGCAAC AGCCGCCGCA TACTCGTAGT GCTGTCACGG TAAATACGAA CGTCAATGTG 
GGAAACAATT TGCAGCCAGT GCAGTCTGGA CCCAATGGAC CTCTCCCTGT TCCGGGGGCT 
AGCTCGTCGC AAATTCAATT GCTCACAAGC ACAGTGCAGC CAGGAGCCAG CACTGTAATG 
GGCGTTGGCG GTCCTGGTAG TACAATGGGG CAAGTGGGTG TGCCACCAAT GGTTGGGGTT 
GGTGTAATGA CAACAAGTGT GCAACCGCAA CCTGTGCAGG TGCAGCCTGC TAGCCGGCGC 
CGGCATCAGC ATCGATTGCA GATAATCGAT CCGACGACTA AAAAAAATAT TTTAGACGAT 
TTTGATAAGA CAAAATCAAA CACAGACAAT GAATTTTCCG ATCAGGTAAC CTCCACAAAC 
ACACCAGCAA CTGTCCTTTC AGAAGGTCCA ATACGTATTC CACAGCAAGA AAGTGTTGGT 
TTAAATAATT TGACCAGTAC ATCATCACAA GGATCAGAAT CACGGACAAA CGCGCCTTAT 
ATCCCCATTG AAACAACACC AATAAGTCGA ACAGATGTTG GGCCGACTCC AATCGTATCC 
GCGATGACTG ATGCTCCTTC GGTTGAAATA TTACCAACGC CCCAAAGAGG GAGAAGCAAA 
AAAATTCCTA TAGTGTCTCC TAAAAATGTA TCTGAGTCCA TAGTTGCTCC TATCACTGAT 
GAAACGGATG ATGCAGGGTC AACACCGATA TCCAGAGCCA CTGAAGAATC TATGCCACCT 
AATCAGACCC ATCCAAATTT ACTCATATCC GATTCATCAA AACATAAGCA AGCAGTATCA 
AATAGTGAGA TTTCAAAAGA CGCTCCTACT GGTTTAAAGG AAATGCATGT TGCTGAGCTG 
GAGTCCAGTG TAGCCTCCGA AAATCATCCA GCTGGAATAC TAGATGTCAA TGTCAACAAG 
AGTTCACAAT CATTGGATTT TTCTGCAGAT CCCGAAATTG ATAGTATTGG CGCAATATCA 
CCTACGAGTC CAAACGCAGT TTCTCCTATT TTACACGAAG TTTTAGATAA TACTGAGTTG 
TCAAAAAAAT TAGAGAATTC TACGACAGAA AGATTTAAAG ATAGCCAAAG CGTAGAGAAG 
CCAACTCATC AAGAACTAAG TTTAAGAAAT GCCACAGACG AGACTGAAAT ATCCGCAATG 
GCATTACAAG ATGTGAATAG TTTGGATAAT AACCAAATTG AAAAAACATA CCCTTCAAAG 
CCGAAACTCA ATGTAGATGT TTCAGAAGAC ATTTCCTCCA GAGAGTCAGC AATAAAGTCA 
ACTTCTACAA AAAATACAGG AGTAGATGTA GGTCTCCAAT CAGATTCTAA GCCAGAGACT 
ACCCTTAACG ATAAACAGGA CTCTACTGAC CTAAAAGTGA AAGTGTCTGC GAAAATTTCA 
TCTATCATAA ACTATAATGA GGGTCAATGG TCACCAAACA ATCCAAGCGG AAAAAAACAA 
TACGACCGAG AACAGTTATT ACAGTTACGC GAGGTTAAGG CCTCACGTAT ACAACCAGAG 
GTAAAAAATG TGTCTATTCT TCCTCAACCA AACTTAATGC CATCATTTAT TCGAAATAAC 
AATAATAACA AGCGAGTACA ATCGATGGTT GGTATTATTG GCAATCGTAG CAATGAATCG 
GCGGGAAATT ATATTGGTAA ACAGATGTCG ATGTCTGGCG TACAAAGTGG CGGCGGTAGA 
AGCAGTATGA AGGGCATGAT ACACGTAAAT TTATCATTAA ATCAAGATGT AAAGTTAAGT 
GAAAATGAAA ATGCATGGCG CCCTCGGGTC TTAAATAAAT CTGATGGTGA TAGCGACGCG 
AAATCTGCAC TCGAAAAAGA TGAATTGGTA CGTCGTGTTC GGGGTATTTT AAATAAGCTT 
ACCCCAGAAA GGTTTGACAC ACTTGTTGAG GAGATTATAA AATTGAAAAT TGATACACCC 
GACAAAGTGG ACGAAGTAAT TGTGTTGGTA TTTGAAAAGG CCATTGATGA GCCAAACTTT 
TCTGTGTCTT ACGCCAGACT ATGTCAACGA CTTGCTGCGG AAGTGAAGGT AATAGACGAG 
CGCATGGAAA GCGAAACCAA ATCTAACTCG GCACACTTTC GAAATGCATT GTTAGATAAA 
ACTGAACAGG AATTCACCCA AAACGTTTCA CAAAGCACCG CAAAGGAAAA AAAATTACAG 
CCAATTGTGG ATAAAATTAA GAAATGTACT GATGCCAATG AAAAAGCGGA ACTTGAGGCA 
TTTTTGGAAG AAGAAGAAAG AAAAATAAGA AGACGATCCG GAGGGACAGT ACGATTTATT 
GGCGAACTTT TTAAGATATC TATGTTGACT GGCAAAATAA TATACTCTTG CATCGATACA 
CTTCTTAACC CTCATTCTGA AGACATGCTT GAGTGTTTGT GCAAGCTGCT TACAACTGTG 
GGTGCTAAAT TTGAAAAGAC CCCAGTAAAT TCCAAAGACC CTAGTCGGTG TTATTCGTTG 
GAAAAATCGA TCACCAGGAT GCAGGCCATC GCATCGAAAA CTGACAAAGA TGGAGCCAGA 
GTAAGCTCAA GAGTACGATT TATGCTTCAA GACGTTATCG ACTTGCGTAA GAATAAATGG 
CAAACTTCGC GGAATGAAGC ACCCAAAACT ATGGGACAAA TTGAAAAAGA GGCAAAGAAT 
GAGCAGCTTT CTGCACAATA TTTTGGTACA CTTTCCAGTA CTACACCCGG AGGATCGCAG 
GGCGGCTCTG GTAAACGAGA TGACCGTGGA AACTCTCGAT ACGGTGAATC TCGTTCAAGT 
AGCGCGTATG GTGGCAGCCA CAGTCAACGT GGTGATAATG GCAACCTTCG ACACCAGCAG 
CAGAACAACG TCGGGGGAAA CGTCTCTGGA GGTGCTGGAC ACTCCAATGG AAACAATGAT 
GAAAATACTT GGCATGTGCA AACCAGCAAA GGTAGCCGCT CTCTAGCGGT TGACAGCAAT 
AAACTGGAAG GCCTGTCTAA ACTTTCTGAC CAAAATCTGG AAACAAAGAA GATGGGTGGA 
TTAACGCAAT TCATTTGGAT TAGTAGTGAC ACGACAAGAC TATCGTCGGC GCCGACACCA 
ACCCCATCCA ATCCTTTCGC TGTGCTCTCA TCCCTAATTG ATAAAAATAG TAATGAAAGG 
GATCGCGATC GCAGTGGACC CAGAAACAAA GGATCATACA ACAAAGGTTC TATGGAGCGG 
GATCGTTATG ATAGAGGTAT GCATTCGAGA ACTGGATCAT CTCAGGGATC ACGAGAAAAC 
TCATCCTCCC GCGGTGGTCA GCAGGGTCGA ACTTTATTGA GTTCATCAGT TCAAAAATCT 
ACTAGTCATT CAAAATACAC CCAACAGGCA CCACCTACAC GGCATACTGT TAAAGCCCAG 
TCATCAGTGG GCAGCTCTAA CGTTAACACA GGTCCACTCT ACCGTGGAAG TGAGCAGCAA 
ACATCAGCAA CCTTCTCGCA GACCACCCGT TCTGTTGCTC CTGTAGCAGT GTTCATCGAA 
GCTAGCGAAA CAGATTTAAA ACTCATCAAA TCTGTTGTTT CAGAAATTGT AGACCTATCA 
GCTGCATCTA AAGAAGTCAC GCCCGGTGCA GTTTCGTGCA TAAAACGAGT ACCAGAGAAA 
CTACGGTGCA GTTTCATTTA TTATATTTTA ACAGATTATT TGCATTTAGC AAATGTAGGT 
AAACAGTACA GGCGGTATTT ATCTATTGCC GTCTCCCAAC TTATTCAGCA GAATTACATT 
TCTGCCGATC ATTTGAGACT GGCTTACAAC GAATTTACCG TATATGCAAA CGATTTGATT 
GTTGATATTC CGGAGCTTTG GCTATACATT CTTCAATTTG CCGGGCCACT AATTGTAAAG 
AAAATATTGA CTATATCAGA TTTGTGGAAC AACAATTTAA AAGAAAATAG TCCATCAAAC 
GTCGCTAAAA AGTTCCTTAA AACATACTTG ATATATTGCA CACAAGAAGT TGGACCAAAT 
TTTGCTCGGA ATATGTGGAT CAAGTTTAAC CTAAAATGGT CAGATTTTAT GCCTGAAAGT 
GAAGTTGCCG ACTTTATTAA ATTTAACAGA CTGGAATATG TGGAAAACGA ATCAAAGTCG 
CCAGTGATTG ATCATAGAGA GACTCCAGAA AAGCACGTTA AAAATGTGAT AGACCATATT 
GAGCACTTAC TAAAGGAAGG AACAACGGCG GACTGCATTA TTGATTATAG TAACGGAAAT 
ATAATGGTGG TTGATAAGTT GTTTATCAGA GGATTGACGG AAACATTGAG TAATTTCTCG 
ATTCACTACA AGGACAACAG TTACAAGCTT GAGTCCGAAA CCTTTCAAAA GTTTTGCATA 
CCGGTTTTGC AGAGATACAT TGATTCTAAT GAAGACCACC AACTTGAATG TTTATATACT 
TTGCAGCTTT TAGTTCATGG ATTGGAGCAT CCAAGAGGGT TGCTGAGTGA ACTAATTGGC 
GAGCTATATG ATGCCTTTGT AATACAGAAG GAATCGCTTT GTAAGTGGCG AGATTCAAAG 
GATCAGTCTG CTGGCAAGGG CGTTGCTGTA AAAAGTCTTA ATCCTTTTTT TAACTCGTTG 
TTAAACGATG ATGCCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4G1 ( NM_001103382.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096852.1 Drosophila melanogaster eukaryotic translation initiation factor 4G1 (eIF4G1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00200
Clone ID Related Accession (Same CDS sequence)	NM_001103382.2
Accession Version	NM_001103382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4G1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001103382.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACAGG CTATACCAAC TTTACCAACA CAATCAGATA TAGATAAAGC CATGCAGCCC 
CATTCAGCAC AAAATATGAT TTTGCCAGCC AATAAAAAGA CCAAAAAGTA TGATCAACAG 
GTGCCAACTT CAAAGCCACA ATCTCTTCAT CAGCCTCTTC AACCGCAGCA TAGCCATCCC 
ACTGCTCAGC CTCAGTTTCA GATAAATAAG GCATACAATG TGGTCTCCAT TCTTAAGGCA 
TCAGCGCAGA TCGCTCAACA GTCCCCACAC TTGACGAATC AGCAGCATCC ACCAATCCAT 
CACCCACAGC AAACACAGCA GCATCAACAA AGCTATACAA ACGTTGTAAA TAGAAGCTTA 
TCTGCGTCTG AACCAGTTAG AGCCCAGTCA TCGGTTATCT GTAACGGTTC AAGCATATTG 
ACAGTAAATA GTAGACAACT GAATTCGGGT GATATGAATT CGACTGCCAT TTATAACATA 
TCCAGTTACC GAAAATTAAC TGGAAGTCTA GATGGAAATG TTTGCTTTCT AAATGTACAG 
GACATTAAAC AAAATGGCAA CATCAGTGGG TCAGTAGTAT CAAATAAGTC CATCGTCGGA 
GTCGGAAGTG AAAAAAGTTC TTGTACTGGA GTCAGTATTA ACAACCAAAT AGTATTGCCT 
AATGCGCAAA TAGGAACATC TATGGGAATA GCTGGAACAA CTACAGCAGG CACAAGCTAC 
ATGCACGAAA AGAATATCGT TGGCGTTAGC GTCAATTGTG TTAATACTAG TAAAAAGTAT 
GATTTCAATA ACAGTAGTTT GTTATCAAAT AATAGTTACC CGGCATCTAC AGCAGAGTAT 
GTATCAACGG GAAACAACAA TTCTGGGAAT ACCCGTTCTA ATCCACAAAG TGGCGGAATA 
TTTCGAGGAC CACCCTCGAC GCCTAATGCC CCTAGAGGTG CTAGTGGCGG AGCAACACGG 
CATGTCCATG TGCAACCCAT GTACTCACAA CCCCTTCACC AGAACATGGT GCTACAACAA 
TACACACAAT ATAACCCACG GCAACAAACA TTTCCAGCTT CTCATTTGCA ATACGCACCC 
GCCCCCATGC CATACTACCA GTACCAATAT GTCCCAACTC TTCAGCAACA GCCGCCGCAT 
ACTCGTAGTG CTGTCACGGT AAATACGAAC GTCAATGTGG GAAACAATTT GCAGCCAGTG 
CAGTCTGGAC CCAATGGACC TCTCCCTGTT CCGGGGGCTA GCTCGTCGCA AATTCAATTG 
CTCACAAGCA CAGTGCAGCC AGGAGCCAGC ACTGTAATGG GCGTTGGCGG TCCTGGTAGT 
ACAATGGGGC AAGTGGGTGT GCCACCAATG GTTGGGGTTG GTGTAATGAC AACAAGTGTG 
CAACCGCAAC CTGTGCAGGT GCAGCCTGCT AGCCGGCGCC GGCATCAGCA TCGATTGCAG 
ATAATCGATC CGACGACTAA AAAAAATATT TTAGACGATT TTGATAAGAC AAAATCAAAC 
ACAGACAATG AATTTTCCGA TCAGGTAACC TCCACAAACA CACCAGCAAC TGTCCTTTCA 
GAAGGTCCAA TACGTATTCC ACAGCAAGAA AGTGTTGGTT TAAATAATTT GACCAGTACA 
TCATCACAAG GATCAGAATC ACGGACAAAC GCGCCTTATA TCCCCATTGA AACAACACCA 
ATAAGTCGAA CAGATGTTGG GCCGACTCCA ATCGTATCCG CGATGACTGA TGCTCCTTCG 
GTTGAAATAT TACCAACGCC CCAAAGAGGG AGAAGCAAAA AAATTCCTAT AGTGTCTCCT 
AAAAATGTAT CTGAGTCCAT AGTTGCTCCT ATCACTGATG AAACGGATGA TGCAGGGTCA 
ACACCGATAT CCAGAGCCAC TGAAGAATCT ATGCCACCTA ATCAGACCCA TCCAAATTTA 
CTCATATCCG ATTCATCAAA ACATAAGCAA GCAGTATCAA ATAGTGAGAT TTCAAAAGAC 
GCTCCTACTG GTTTAAAGGA AATGCATGTT GCTGAGCTGG AGTCCAGTGT AGCCTCCGAA 
AATCATCCAG CTGGAATACT AGATGTCAAT GTCAACAAGA GTTCACAATC ATTGGATTTT 
TCTGCAGATC CCGAAATTGA TAGTATTGGC GCAATATCAC CTACGAGTCC AAACGCAGTT 
TCTCCTATTT TACACGAAGT TTTAGATAAT ACTGAGTTGT CAAAAAAATT AGAGAATTCT 
ACGACAGAAA GATTTAAAGA TAGCCAAAGC GTAGAGAAGC CAACTCATCA AGAACTAAGT 
TTAAGAAATG CCACAGACGA GACTGAAATA TCCGCAATGG CATTACAAGA TGTGAATAGT 
TTGGATAATA ACCAAATTGA AAAAACATAC CCTTCAAAGC CGAAACTCAA TGTAGATGTT 
TCAGAAGACA TTTCCTCCAG AGAGTCAGCA ATAAAGTCAA CTTCTACAAA AAATACAGGA 
GTAGATGTAG GTCTCCAATC AGATTCTAAG CCAGAGACTA CCCTTAACGA TAAACAGGAC 
TCTACTGACC TAAAAGTGAA AGTGTCTGCG AAAATTTCAT CTATCATAAA CTATAATGAG 
GGTCAATGGT CACCAAACAA TCCAAGCGGA AAAAAACAAT ACGACCGAGA ACAGTTATTA 
CAGTTACGCG AGGTTAAGGC CTCACGTATA CAACCAGAGG TAAAAAATGT GTCTATTCTT 
CCTCAACCAA ACTTAATGCC ATCATTTATT CGAAATAACA ATAATAACAA GCGAGTACAA 
TCGATGGTTG GTATTATTGG CAATCGTAGC AATGAATCGG CGGGAAATTA TATTGGTAAA 
CAGATGTCGA TGTCTGGCGT ACAAAGTGGC GGCGGTAGAA GCAGTATGAA GGGCATGATA 
CACGTAAATT TATCATTAAA TCAAGATGTA AAGTTAAGTG AAAATGAAAA TGCATGGCGC 
CCTCGGGTCT TAAATAAATC TGATGGTGAT AGCGACGCGA AATCTGCACT CGAAAAAGAT 
GAATTGGTAC GTCGTGTTCG GGGTATTTTA AATAAGCTTA CCCCAGAAAG GTTTGACACA 
CTTGTTGAGG AGATTATAAA ATTGAAAATT GATACACCCG ACAAAGTGGA CGAAGTAATT 
GTGTTGGTAT TTGAAAAGGC CATTGATGAG CCAAACTTTT CTGTGTCTTA CGCCAGACTA 
TGTCAACGAC TTGCTGCGGA AGTGAAGGTA ATAGACGAGC GCATGGAAAG CGAAACCAAA 
TCTAACTCGG CACACTTTCG AAATGCATTG TTAGATAAAA CTGAACAGGA ATTCACCCAA 
AACGTTTCAC AAAGCACCGC AAAGGAAAAA AAATTACAGC CAATTGTGGA TAAAATTAAG 
AAATGTACTG ATGCCAATGA AAAAGCGGAA CTTGAGGCAT TTTTGGAAGA AGAAGAAAGA 
AAAATAAGAA GACGATCCGG AGGGACAGTA CGATTTATTG GCGAACTTTT TAAGATATCT 
ATGTTGACTG GCAAAATAAT ATACTCTTGC ATCGATACAC TTCTTAACCC TCATTCTGAA 
GACATGCTTG AGTGTTTGTG CAAGCTGCTT ACAACTGTGG GTGCTAAATT TGAAAAGACC 
CCAGTAAATT CCAAAGACCC TAGTCGGTGT TATTCGTTGG AAAAATCGAT CACCAGGATG 
CAGGCCATCG CATCGAAAAC TGACAAAGAT GGAGCCAGAG TAAGCTCAAG AGTACGATTT 
ATGCTTCAAG ACGTTATCGA CTTGCGTAAG AATAAATGGC AAACTTCGCG GAATGAAGCA 
CCCAAAACTA TGGGACAAAT TGAAAAAGAG GCAAAGAATG AGCAGCTTTC TGCACAATAT 
TTTGGTACAC TTTCCAGTAC TACACCCGGA GGATCGCAGG GCGGCTCTGG TAAACGAGAT 
GACCGTGGAA ACTCTCGATA CGGTGAATCT CGTTCAAGTA GCGCGTATGG TGGCAGCCAC 
AGTCAACGTG GTGATAATGG CAACCTTCGA CACCAGCAGC AGAACAACGT CGGGGGAAAC 
GTCTCTGGAG GTGCTGGACA CTCCAATGGA AACAATGATG AAAATACTTG GCATGTGCAA 
ACCAGCAAAG GTAGCCGCTC TCTAGCGGTT GACAGCAATA AACTGGAAGG CCTGTCTAAA 
CTTTCTGACC AAAATCTGGA AACAAAGAAG ATGGGTGGAT TAACGCAATT CATTTGGATT 
AGTAGTGACA CGACAAGACT ATCGTCGGCG CCGACACCAA CCCCATCCAA TCCTTTCGCT 
GTGCTCTCAT CCCTAATTGA TAAAAATAGT AATGAAAGGG ATCGCGATCG CAGTGGACCC 
AGAAACAAAG GATCATACAA CAAAGGTTCT ATGGAGCGGG ATCGTTATGA TAGAGGTATG 
CATTCGAGAA CTGGATCATC TCAGGGATCA CGAGAAAACT CATCCTCCCG CGGTGGTCAG 
CAGGGTCGAA CTTTATTGAG TTCATCAGTT CAAAAATCTA CTAGTCATTC AAAATACACC 
CAACAGGCAC CACCTACACG GCATACTGTT AAAGCCCAGT CATCAGTGGG CAGCTCTAAC 
GTTAACACAG GTCCACTCTA CCGTGGAAGT GAGCAGCAAA CATCAGCAAC CTTCTCGCAG 
ACCACCCGTT CTGTTGCTCC TGTAGCAGTG TTCATCGAAG CTAGCGAAAC AGATTTAAAA 
CTCATCAAAT CTGTTGTTTC AGAAATTGTA GACCTATCAG CTGCATCTAA AGAAGTCACG 
CCCGGTGCAG TTTCGTGCAT AAAACGAGTA CCAGAGAAAC TACGGTGCAG TTTCATTTAT 
TATATTTTAA CAGATTATTT GCATTTAGCA AATGTAGGTA AACAGTACAG GCGGTATTTA 
TCTATTGCCG TCTCCCAACT TATTCAGCAG AATTACATTT CTGCCGATCA TTTGAGACTG 
GCTTACAACG AATTTACCGT ATATGCAAAC GATTTGATTG TTGATATTCC GGAGCTTTGG 
CTATACATTC TTCAATTTGC CGGGCCACTA ATTGTAAAGA AAATATTGAC TATATCAGAT 
TTGTGGAACA ACAATTTAAA AGAAAATAGT CCATCAAACG TCGCTAAAAA GTTCCTTAAA 
ACATACTTGA TATATTGCAC ACAAGAAGTT GGACCAAATT TTGCTCGGAA TATGTGGATC 
AAGTTTAACC TAAAATGGTC AGATTTTATG CCTGAAAGTG AAGTTGCCGA CTTTATTAAA 
TTTAACAGAC TGGAATATGT GGAAAACGAA TCAAAGTCGC CAGTGATTGA TCATAGAGAG 
ACTCCAGAAA AGCACGTTAA AAATGTGATA GACCATATTG AGCACTTACT AAAGGAAGGA 
ACAACGGCGG ACTGCATTAT TGATTATAGT AACGGAAATA TAATGGTGGT TGATAAGTTG 
TTTATCAGAG GATTGACGGA AACATTGAGT AATTTCTCGA TTCACTACAA GGACAACAGT 
TACAAGCTTG AGTCCGAAAC CTTTCAAAAG TTTTGCATAC CGGTTTTGCA GAGATACATT 
GATTCTAATG AAGACCACCA ACTTGAATGT TTATATACTT TGCAGCTTTT AGTTCATGGA 
TTGGAGCATC CAAGAGGGTT GCTGAGTGAA CTAATTGGCG AGCTATATGA TGCCTTTGTA 
ATACAGAAGG AATCGCTTTG TAAGTGGCGA GATTCAAAGG ATCAGTCTGC TGGCAAGGGC 
GTTGCTGTAA AAAGTCTTAA TCCTTTTTTT AACTCGTTGT TAAACGATGA TGCCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096852.1
CDS	381..6140
Translation	MQQAIPTLPTQSDIDKAMQPHSAQNMILPANKKTKKYDQQVPTSKPQSLHQPLQPQHSHPTAQPQFQINKAYNVVSILKASAQIAQQSPHLTNQQHPPIHHPQQTQQHQQSYTNVVNRSLSASEPVRAQSSVICNGSSILTVNSRQLNSGDMNSTAIYNISSYRKLTGSLDGNVCFLNVQDIKQNGNISGSVVSNKSIVGVGSEKSSCTGVSINNQIVLPNAQIGTSMGIAGTTTAGTSYMHEKNIVGVSVNCVNTSKKYDFNNSSLLSNNSYPASTAEYVSTGNNNSGNTRSNPQSGGIFRGPPSTPNAPRGASGGATRHVHVQPMYSQPLHQNMVLQQYTQYNPRQQTFPASHLQYAPAPMPYYQYQYVPTLQQQPPHTRSAVTVNTNVNVGNNLQPVQSGPNGPLPVPGASSSQIQLLTSTVQPGASTVMGVGGPGSTMGQVGVPPMVGVGVMTTSVQPQPVQVQPASRRRHQHRLQIIDPTTKKNILDDFDKTKSNTDNEFSDQVTSTNTPATVLSEGPIRIPQQESVGLNNLTSTSSQGSESRTNAPYIPIETTPISRTDVGPTPIVSAMTDAPSVEILPTPQRGRSKKIPIVSPKNVSESIVAPITDETDDAGSTPISRATEESMPPNQTHPNLLISDSSKHKQAVSNSEISKDAPTGLKEMHVAELESSVASENHPAGILDVNVNKSSQSLDFSADPEIDSIGAISPTSPNAVSPILHEVLDNTELSKKLENSTTERFKDSQSVEKPTHQELSLRNATDETEISAMALQDVNSLDNNQIEKTYPSKPKLNVDVSEDISSRESAIKSTSTKNTGVDVGLQSDSKPETTLNDKQDSTDLKVKVSAKISSIINYNEGQWSPNNPSGKKQYDREQLLQLREVKASRIQPEVKNVSILPQPNLMPSFIRNNNNNKRVQSMVGIIGNRSNESAGNYIGKQMSMSGVQSGGGRSSMKGMIHVNLSLNQDVKLSENENAWRPRVLNKSDGDSDAKSALEKDELVRRVRGILNKLTPERFDTLVEEIIKLKIDTPDKVDEVIVLVFEKAIDEPNFSVSYARLCQRLAAEVKVIDERMESETKSNSAHFRNALLDKTEQEFTQNVSQSTAKEKKLQPIVDKIKKCTDANEKAELEAFLEEEERKIRRRSGGTVRFIGELFKISMLTGKIIYSCIDTLLNPHSEDMLECLCKLLTTVGAKFEKTPVNSKDPSRCYSLEKSITRMQAIASKTDKDGARVSSRVRFMLQDVIDLRKNKWQTSRNEAPKTMGQIEKEAKNEQLSAQYFGTLSSTTPGGSQGGSGKRDDRGNSRYGESRSSSAYGGSHSQRGDNGNLRHQQQNNVGGNVSGGAGHSNGNNDENTWHVQTSKGSRSLAVDSNKLEGLSKLSDQNLETKKMGGLTQFIWISSDTTRLSSAPTPTPSNPFAVLSSLIDKNSNERDRDRSGPRNKGSYNKGSMERDRYDRGMHSRTGSSQGSRENSSSRGGQQGRTLLSSSVQKSTSHSKYTQQAPPTRHTVKAQSSVGSSNVNTGPLYRGSEQQTSATFSQTTRSVAPVAVFIEASETDLKLIKSVVSEIVDLSAASKEVTPGAVSCIKRVPEKLRCSFIYYILTDYLHLANVGKQYRRYLSIAVSQLIQQNYISADHLRLAYNEFTVYANDLIVDIPELWLYILQFAGPLIVKKILTISDLWNNNLKENSPSNVAKKFLKTYLIYCTQEVGPNFARNMWIKFNLKWSDFMPESEVADFIKFNRLEYVENESKSPVIDHRETPEKHVKNVIDHIEHLLKEGTTADCIIDYSNGNIMVVDKLFIRGLTETLSNFSIHYKDNSYKLESETFQKFCIPVLQRYIDSNEDHQLECLYTLQLLVHGLEHPRGLLSELIGELYDAFVIQKESLCKWRDSKDQSAGKGVAVKSLNPFFNSLLNDDAN

Target ORF information:

RefSeq Version	NM_001103382.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4G1 (eIF4G1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103382.2

ORF Insert Sequence:

ATGCAACAGG CTATACCAAC TTTACCAACA CAATCAGATA TAGATAAAGC CATGCAGCCC 
CATTCAGCAC AAAATATGAT TTTGCCAGCC AATAAAAAGA CCAAAAAGTA TGATCAACAG 
GTGCCAACTT CAAAGCCACA ATCTCTTCAT CAGCCTCTTC AACCGCAGCA TAGCCATCCC 
ACTGCTCAGC CTCAGTTTCA GATAAATAAG GCATACAATG TGGTCTCCAT TCTTAAGGCA 
TCAGCGCAGA TCGCTCAACA GTCCCCACAC TTGACGAATC AGCAGCATCC ACCAATCCAT 
CACCCACAGC AAACACAGCA GCATCAACAA AGCTATACAA ACGTTGTAAA TAGAAGCTTA 
TCTGCGTCTG AACCAGTTAG AGCCCAGTCA TCGGTTATCT GTAACGGTTC AAGCATATTG 
ACAGTAAATA GTAGACAACT GAATTCGGGT GATATGAATT CGACTGCCAT TTATAACATA 
TCCAGTTACC GAAAATTAAC TGGAAGTCTA GATGGAAATG TTTGCTTTCT AAATGTACAG 
GACATTAAAC AAAATGGCAA CATCAGTGGG TCAGTAGTAT CAAATAAGTC CATCGTCGGA 
GTCGGAAGTG AAAAAAGTTC TTGTACTGGA GTCAGTATTA ACAACCAAAT AGTATTGCCT 
AATGCGCAAA TAGGAACATC TATGGGAATA GCTGGAACAA CTACAGCAGG CACAAGCTAC 
ATGCACGAAA AGAATATCGT TGGCGTTAGC GTCAATTGTG TTAATACTAG TAAAAAGTAT 
GATTTCAATA ACAGTAGTTT GTTATCAAAT AATAGTTACC CGGCATCTAC AGCAGAGTAT 
GTATCAACGG GAAACAACAA TTCTGGGAAT ACCCGTTCTA ATCCACAAAG TGGCGGAATA 
TTTCGAGGAC CACCCTCGAC GCCTAATGCC CCTAGAGGTG CTAGTGGCGG AGCAACACGG 
CATGTCCATG TGCAACCCAT GTACTCACAA CCCCTTCACC AGAACATGGT GCTACAACAA 
TACACACAAT ATAACCCACG GCAACAAACA TTTCCAGCTT CTCATTTGCA ATACGCACCC 
GCCCCCATGC CATACTACCA GTACCAATAT GTCCCAACTC TTCAGCAACA GCCGCCGCAT 
ACTCGTAGTG CTGTCACGGT AAATACGAAC GTCAATGTGG GAAACAATTT GCAGCCAGTG 
CAGTCTGGAC CCAATGGACC TCTCCCTGTT CCGGGGGCTA GCTCGTCGCA AATTCAATTG 
CTCACAAGCA CAGTGCAGCC AGGAGCCAGC ACTGTAATGG GCGTTGGCGG TCCTGGTAGT 
ACAATGGGGC AAGTGGGTGT GCCACCAATG GTTGGGGTTG GTGTAATGAC AACAAGTGTG 
CAACCGCAAC CTGTGCAGGT GCAGCCTGCT AGCCGGCGCC GGCATCAGCA TCGATTGCAG 
ATAATCGATC CGACGACTAA AAAAAATATT TTAGACGATT TTGATAAGAC AAAATCAAAC 
ACAGACAATG AATTTTCCGA TCAGGTAACC TCCACAAACA CACCAGCAAC TGTCCTTTCA 
GAAGGTCCAA TACGTATTCC ACAGCAAGAA AGTGTTGGTT TAAATAATTT GACCAGTACA 
TCATCACAAG GATCAGAATC ACGGACAAAC GCGCCTTATA TCCCCATTGA AACAACACCA 
ATAAGTCGAA CAGATGTTGG GCCGACTCCA ATCGTATCCG CGATGACTGA TGCTCCTTCG 
GTTGAAATAT TACCAACGCC CCAAAGAGGG AGAAGCAAAA AAATTCCTAT AGTGTCTCCT 
AAAAATGTAT CTGAGTCCAT AGTTGCTCCT ATCACTGATG AAACGGATGA TGCAGGGTCA 
ACACCGATAT CCAGAGCCAC TGAAGAATCT ATGCCACCTA ATCAGACCCA TCCAAATTTA 
CTCATATCCG ATTCATCAAA ACATAAGCAA GCAGTATCAA ATAGTGAGAT TTCAAAAGAC 
GCTCCTACTG GTTTAAAGGA AATGCATGTT GCTGAGCTGG AGTCCAGTGT AGCCTCCGAA 
AATCATCCAG CTGGAATACT AGATGTCAAT GTCAACAAGA GTTCACAATC ATTGGATTTT 
TCTGCAGATC CCGAAATTGA TAGTATTGGC GCAATATCAC CTACGAGTCC AAACGCAGTT 
TCTCCTATTT TACACGAAGT TTTAGATAAT ACTGAGTTGT CAAAAAAATT AGAGAATTCT 
ACGACAGAAA GATTTAAAGA TAGCCAAAGC GTAGAGAAGC CAACTCATCA AGAACTAAGT 
TTAAGAAATG CCACAGACGA GACTGAAATA TCCGCAATGG CATTACAAGA TGTGAATAGT 
TTGGATAATA ACCAAATTGA AAAAACATAC CCTTCAAAGC CGAAACTCAA TGTAGATGTT 
TCAGAAGACA TTTCCTCCAG AGAGTCAGCA ATAAAGTCAA CTTCTACAAA AAATACAGGA 
GTAGATGTAG GTCTCCAATC AGATTCTAAG CCAGAGACTA CCCTTAACGA TAAACAGGAC 
TCTACTGACC TAAAAGTGAA AGTGTCTGCG AAAATTTCAT CTATCATAAA CTATAATGAG 
GGTCAATGGT CACCAAACAA TCCAAGCGGA AAAAAACAAT ACGACCGAGA ACAGTTATTA 
CAGTTACGCG AGGTTAAGGC CTCACGTATA CAACCAGAGG TAAAAAATGT GTCTATTCTT 
CCTCAACCAA ACTTAATGCC ATCATTTATT CGAAATAACA ATAATAACAA GCGAGTACAA 
TCGATGGTTG GTATTATTGG CAATCGTAGC AATGAATCGG CGGGAAATTA TATTGGTAAA 
CAGATGTCGA TGTCTGGCGT ACAAAGTGGC GGCGGTAGAA GCAGTATGAA GGGCATGATA 
CACGTAAATT TATCATTAAA TCAAGATGTA AAGTTAAGTG AAAATGAAAA TGCATGGCGC 
CCTCGGGTCT TAAATAAATC TGATGGTGAT AGCGACGCGA AATCTGCACT CGAAAAAGAT 
GAATTGGTAC GTCGTGTTCG GGGTATTTTA AATAAGCTTA CCCCAGAAAG GTTTGACACA 
CTTGTTGAGG AGATTATAAA ATTGAAAATT GATACACCCG ACAAAGTGGA CGAAGTAATT 
GTGTTGGTAT TTGAAAAGGC CATTGATGAG CCAAACTTTT CTGTGTCTTA CGCCAGACTA 
TGTCAACGAC TTGCTGCGGA AGTGAAGGTA ATAGACGAGC GCATGGAAAG CGAAACCAAA 
TCTAACTCGG CACACTTTCG AAATGCATTG TTAGATAAAA CTGAACAGGA ATTCACCCAA 
AACGTTTCAC AAAGCACCGC AAAGGAAAAA AAATTACAGC CAATTGTGGA TAAAATTAAG 
AAATGTACTG ATGCCAATGA AAAAGCGGAA CTTGAGGCAT TTTTGGAAGA AGAAGAAAGA 
AAAATAAGAA GACGATCCGG AGGGACAGTA CGATTTATTG GCGAACTTTT TAAGATATCT 
ATGTTGACTG GCAAAATAAT ATACTCTTGC ATCGATACAC TTCTTAACCC TCATTCTGAA 
GACATGCTTG AGTGTTTGTG CAAGCTGCTT ACAACTGTGG GTGCTAAATT TGAAAAGACC 
CCAGTAAATT CCAAAGACCC TAGTCGGTGT TATTCGTTGG AAAAATCGAT CACCAGGATG 
CAGGCCATCG CATCGAAAAC TGACAAAGAT GGAGCCAGAG TAAGCTCAAG AGTACGATTT 
ATGCTTCAAG ACGTTATCGA CTTGCGTAAG AATAAATGGC AAACTTCGCG GAATGAAGCA 
CCCAAAACTA TGGGACAAAT TGAAAAAGAG GCAAAGAATG AGCAGCTTTC TGCACAATAT 
TTTGGTACAC TTTCCAGTAC TACACCCGGA GGATCGCAGG GCGGCTCTGG TAAACGAGAT 
GACCGTGGAA ACTCTCGATA CGGTGAATCT CGTTCAAGTA GCGCGTATGG TGGCAGCCAC 
AGTCAACGTG GTGATAATGG CAACCTTCGA CACCAGCAGC AGAACAACGT CGGGGGAAAC 
GTCTCTGGAG GTGCTGGACA CTCCAATGGA AACAATGATG AAAATACTTG GCATGTGCAA 
ACCAGCAAAG GTAGCCGCTC TCTAGCGGTT GACAGCAATA AACTGGAAGG CCTGTCTAAA 
CTTTCTGACC AAAATCTGGA AACAAAGAAG ATGGGTGGAT TAACGCAATT CATTTGGATT 
AGTAGTGACA CGACAAGACT ATCGTCGGCG CCGACACCAA CCCCATCCAA TCCTTTCGCT 
GTGCTCTCAT CCCTAATTGA TAAAAATAGT AATGAAAGGG ATCGCGATCG CAGTGGACCC 
AGAAACAAAG GATCATACAA CAAAGGTTCT ATGGAGCGGG ATCGTTATGA TAGAGGTATG 
CATTCGAGAA CTGGATCATC TCAGGGATCA CGAGAAAACT CATCCTCCCG CGGTGGTCAG 
CAGGGTCGAA CTTTATTGAG TTCATCAGTT CAAAAATCTA CTAGTCATTC AAAATACACC 
CAACAGGCAC CACCTACACG GCATACTGTT AAAGCCCAGT CATCAGTGGG CAGCTCTAAC 
GTTAACACAG GTCCACTCTA CCGTGGAAGT GAGCAGCAAA CATCAGCAAC CTTCTCGCAG 
ACCACCCGTT CTGTTGCTCC TGTAGCAGTG TTCATCGAAG CTAGCGAAAC AGATTTAAAA 
CTCATCAAAT CTGTTGTTTC AGAAATTGTA GACCTATCAG CTGCATCTAA AGAAGTCACG 
CCCGGTGCAG TTTCGTGCAT AAAACGAGTA CCAGAGAAAC TACGGTGCAG TTTCATTTAT 
TATATTTTAA CAGATTATTT GCATTTAGCA AATGTAGGTA AACAGTACAG GCGGTATTTA 
TCTATTGCCG TCTCCCAACT TATTCAGCAG AATTACATTT CTGCCGATCA TTTGAGACTG 
GCTTACAACG AATTTACCGT ATATGCAAAC GATTTGATTG TTGATATTCC GGAGCTTTGG 
CTATACATTC TTCAATTTGC CGGGCCACTA ATTGTAAAGA AAATATTGAC TATATCAGAT 
TTGTGGAACA ACAATTTAAA AGAAAATAGT CCATCAAACG TCGCTAAAAA GTTCCTTAAA 
ACATACTTGA TATATTGCAC ACAAGAAGTT GGACCAAATT TTGCTCGGAA TATGTGGATC 
AAGTTTAACC TAAAATGGTC AGATTTTATG CCTGAAAGTG AAGTTGCCGA CTTTATTAAA 
TTTAACAGAC TGGAATATGT GGAAAACGAA TCAAAGTCGC CAGTGATTGA TCATAGAGAG 
ACTCCAGAAA AGCACGTTAA AAATGTGATA GACCATATTG AGCACTTACT AAAGGAAGGA 
ACAACGGCGG ACTGCATTAT TGATTATAGT AACGGAAATA TAATGGTGGT TGATAAGTTG 
TTTATCAGAG GATTGACGGA AACATTGAGT AATTTCTCGA TTCACTACAA GGACAACAGT 
TACAAGCTTG AGTCCGAAAC CTTTCAAAAG TTTTGCATAC CGGTTTTGCA GAGATACATT 
GATTCTAATG AAGACCACCA ACTTGAATGT TTATATACTT TGCAGCTTTT AGTTCATGGA 
TTGGAGCATC CAAGAGGGTT GCTGAGTGAA CTAATTGGCG AGCTATATGA TGCCTTTGTA 
ATACAGAAGG AATCGCTTTG TAAGTGGCGA GATTCAAAGG ATCAGTCTGC TGGCAAGGGC 
GTTGCTGTAA AAAGTCTTAA TCCTTTTTTT AACTCGTTGT TAAACGATGA TGCCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eIF4G1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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