toy cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	toy
Entrez Gene ID	43833
Full Name	twin of eyeless
Synonyms	CG11186,Dmel\CG11186,Pax-6,TOY,Toy,l(4)102CDg,l(4)8
General protein information	Preferred Names twin of eyeless Names CG11186 gene product from transcript CG11186-RC CG11186-PA CG11186-PC eyeless lethal(4)102CDg toy-PA toy-PC twin-of-eyeless
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102F4-102F5\|4-0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272151.1	NP_001259080.1	twin of eyeless, isoform C
NM_079899.4	NP_524638.3	twin of eyeless, isoform A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pax6	NP_037133.1
Gallus gallus (chicken)	PAX6	NP_990397.1
Drosophila melanogaster (fruit fly)	toy	NP_524638.3
	PAX6	XP_001084865.2
Mus musculus (house mouse)	Pax6	NP_001231127.1
Xenopus tropicalis (tropical clawed frog)	pax6	NP_001006763.1
Bos taurus (cattle)	PAX6	NP_001035735.1
Caenorhabditis elegans (roundworm)	vab-3	NP_001024570.1
Homo sapiens (human)	PAX6	NP_001595.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000067	XP_311087.5
Pan troglodytes (chimpanzee)	PAX6	XP_003954413.1
Danio rerio (zebrafish)	pax6a	NP_571379.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results indicate that the head-specific gap gene empty spiracles can act as a repressor of Toy, while ocelliless (oc) and spalt major (salm) appear to act as positive regulators of toy gene expression.
Title: Regulation of twin of eyeless during Drosophila development.

Data indicate that Hipk as a repressor of both Twin of eyeless (Toy) and Eyeless (Ey).
Title: Hipk promotes photoreceptor differentiation through the repression of Twin of eyeless and Eyeless expression.

Regulation of ocellar specification and size by twin of eyeless and homothorax.
Title: Regulation of ocellar specification and size by twin of eyeless and homothorax.

Mutations of the twin of eyeless gene cause defects in embryonic brain patterning as well as in post-embryonic development of the mushroom bodies.
Title: Brain patterning defects caused by mutations of the twin of eyeless gene in Drosophila melanogaster.

Toy and Ey, to some extent, can substitute for each other.
Title: The Drosophila Pax6 paralogs have different functions in head development but can partially substitute for each other.

The following toy gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the toy cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00195	NM_079899.4 Latest version!	Drosophila melanogaster twin of eyeless (toy), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa00320	NM_001272151.1 Latest version!	Drosophila melanogaster twin of eyeless (toy), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

toy ( NM_079899.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524638.3 Drosophila melanogaster twin of eyeless (toy), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00195
Clone ID Related Accession (Same CDS sequence)	NM_079899.4
Accession Version	NM_079899.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twin of eyeless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_079899.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCTAA CAACTGAACA CATAATGCAT GGGCATCCCC ACTCGTCAGT CGGGCAGAGT 
ACTCTATTTG GGTGCTCCAC GGCGGGCCAT AGCGGAATAA ATCAGCTGGG CGGCGTATAT 
GTTAATGGCC GGCCACTGCC CGATTCAACG CGTCAAAAAA TTGTCGAATT GGCTCATTCC 
GGCGCACGTC CTTGTGATAT TTCAAGAATA CTACAAGTGT CCAACGGTTG CGTAAGCAAA 
ATTTTGGGCA GATATTATGA AACTGGATCG ATAAAACCTC GAGCTATAGG TGGTTCAAAG 
CCACGAGTAG CTACAACCCC GGTTGTGCAA AAAATTGCAG ATTACAAACG GGAATGTCCC 
AGCATATTTG CGTGGGAAAT ACGAGATCGA CTGCTATCGG AACAAGTTTG CAATAGTGAT 
AACATTCCAA GTGTTTCATC TATTAATCGA GTCTTACGTA ACCTGGCCTC ACAAAAGGAG 
CAGCAAGCTC AGCAACAAAA CGAATCCGTT TATGAAAAGC TTCGCATGTT TAATGGCCAA 
ACGGGCGGAT GGGCATGGTA TCCAAGCAAT ACAACGACGG CACATTTGAC GCTACCACCA 
GCAGCTTCCG TTGTGACATC TCCTGCAAAT TTATCAGGAC AGGCCGATCG GGATGATGTT 
CAAAAAAGAG AATTACAATT TTCAGTAGAA GTTTCGCATA CAAACTCTCA CGATAGTACA 
TCGGATGGAA ACTCTGAACA TAATTCATCC GGGGACGAAG ACTCTCAAAT GCGGTTGCGC 
CTAAAAAGGA AGTTACAGCG CAATCGGACA TCATTTTCTA ATGAGCAAAT TGACAGTCTT 
GAAAAAGAAT TTGAAAGAAC ACATTATCCC GATGTTTTTG CGCGAGAAAG GCTTGCTGAT 
AAAATTGGTT TGCCAGAGGC ACGTATTCAG GTTTGGTTTT CAAACCGACG AGCTAAATGG 
CGCCGAGAAG AAAAAATGCG AACTCAGAGA CGATCGGCCG ATACCGTGGA CGGCAGTGGT 
CGAACCAGCA CGGCAAATAA TCCTTCAGGA ACAACTGCAT CTTCCTCCGT CGCAACGTCA 
AACAACTCGA CTCCAGGGAT TGTGAACTCA GCAATCAACG TTGCGGAACG AACATCATCT 
GCATTAGTTA GTAATAGCCT TCCCGAGGCT TCAAATGGAC CAACTGTTTT GGGTGGTGAA 
GCTAATACTA CACACACCAG CTCTGAAAGC CCACCCCTTC AGCCAGCGGC ACCGCGGCTA 
CCCTTAAATT CTGGATTCAA CACCATGTAC TCATCTATTC CACAACCGAT TGCAACGATG 
GCTGAAAATT ACAACTCCTC ATTAGGATCA ATGACCCCGT CATGCTTACA ACAACGCGAT 
GCCTATCCTT ACATGTTTCA CGATCCGTTA TCACTAGGAT CTCCCTATGT GTCAGCCCAC 
CATCGAAACA CAGCTTGCAA CCCCTCAGCT GCGCACCAAC AGCCCCCTCA GCATGGCGTT 
TATACCAATA GTTCTCCAAT GCCATCATCA AACACAGGTG TCATTTCTGC GGGCGTTTCG 
GTGCCTGTCC AGATTTCAAC GCAAAATGTA TCTGACCTAA CGGGAAGCAA TTACTGGCCA 
CGTCTTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524638.3
CDS	528..2159
Translation	MMLTTEHIMHGHPHSSVGQSTLFGCSTAGHSGINQLGGVYVNGRPLPDSTRQKIVELAHSGARPCDISRILQVSNGCVSKILGRYYETGSIKPRAIGGSKPRVATTPVVQKIADYKRECPSIFAWEIRDRLLSEQVCNSDNIPSVSSINRVLRNLASQKEQQAQQQNESVYEKLRMFNGQTGGWAWYPSNTTTAHLTLPPAASVVTSPANLSGQADRDDVQKRELQFSVEVSHTNSHDSTSDGNSEHNSSGDEDSQMRLRLKRKLQRNRTSFSNEQIDSLEKEFERTHYPDVFARERLADKIGLPEARIQVWFSNRRAKWRREEKMRTQRRSADTVDGSGRTSTANNPSGTTASSSVATSNNSTPGIVNSAINVAERTSSALVSNSLPEASNGPTVLGGEANTTHTSSESPPLQPAAPRLPLNSGFNTMYSSIPQPIATMAENYNSSLGSMTPSCLQQRDAYPYMFHDPLSLGSPYVSAHHRNTACNPSAAHQQPPQHGVYTNSSPMPSSNTGVISAGVSVPVQISTQNVSDLTGSNYWPRLQ

Target ORF information:

RefSeq Version	NM_079899.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twin of eyeless (toy), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079899.4

ORF Insert Sequence:

ATGATGCTAA CAACTGAACA CATAATGCAT GGGCATCCCC ACTCGTCAGT CGGGCAGAGT 
ACTCTATTTG GGTGCTCCAC GGCGGGCCAT AGCGGAATAA ATCAGCTGGG CGGCGTATAT 
GTTAATGGCC GGCCACTGCC CGATTCAACG CGTCAAAAAA TTGTCGAATT GGCTCATTCC 
GGCGCACGTC CTTGTGATAT TTCAAGAATA CTACAAGTGT CCAACGGTTG CGTAAGCAAA 
ATTTTGGGCA GATATTATGA AACTGGATCG ATAAAACCTC GAGCTATAGG TGGTTCAAAG 
CCACGAGTAG CTACAACCCC GGTTGTGCAA AAAATTGCAG ATTACAAACG GGAATGTCCC 
AGCATATTTG CGTGGGAAAT ACGAGATCGA CTGCTATCGG AACAAGTTTG CAATAGTGAT 
AACATTCCAA GTGTTTCATC TATTAATCGA GTCTTACGTA ACCTGGCCTC ACAAAAGGAG 
CAGCAAGCTC AGCAACAAAA CGAATCCGTT TATGAAAAGC TTCGCATGTT TAATGGCCAA 
ACGGGCGGAT GGGCATGGTA TCCAAGCAAT ACAACGACGG CACATTTGAC GCTACCACCA 
GCAGCTTCCG TTGTGACATC TCCTGCAAAT TTATCAGGAC AGGCCGATCG GGATGATGTT 
CAAAAAAGAG AATTACAATT TTCAGTAGAA GTTTCGCATA CAAACTCTCA CGATAGTACA 
TCGGATGGAA ACTCTGAACA TAATTCATCC GGGGACGAAG ACTCTCAAAT GCGGTTGCGC 
CTAAAAAGGA AGTTACAGCG CAATCGGACA TCATTTTCTA ATGAGCAAAT TGACAGTCTT 
GAAAAAGAAT TTGAAAGAAC ACATTATCCC GATGTTTTTG CGCGAGAAAG GCTTGCTGAT 
AAAATTGGTT TGCCAGAGGC ACGTATTCAG GTTTGGTTTT CAAACCGACG AGCTAAATGG 
CGCCGAGAAG AAAAAATGCG AACTCAGAGA CGATCGGCCG ATACCGTGGA CGGCAGTGGT 
CGAACCAGCA CGGCAAATAA TCCTTCAGGA ACAACTGCAT CTTCCTCCGT CGCAACGTCA 
AACAACTCGA CTCCAGGGAT TGTGAACTCA GCAATCAACG TTGCGGAACG AACATCATCT 
GCATTAGTTA GTAATAGCCT TCCCGAGGCT TCAAATGGAC CAACTGTTTT GGGTGGTGAA 
GCTAATACTA CACACACCAG CTCTGAAAGC CCACCCCTTC AGCCAGCGGC ACCGCGGCTA 
CCCTTAAATT CTGGATTCAA CACCATGTAC TCATCTATTC CACAACCGAT TGCAACGATG 
GCTGAAAATT ACAACTCCTC ATTAGGATCA ATGACCCCGT CATGCTTACA ACAACGCGAT 
GCCTATCCTT ACATGTTTCA CGATCCGTTA TCACTAGGAT CTCCCTATGT GTCAGCCCAC 
CATCGAAACA CAGCTTGCAA CCCCTCAGCT GCGCACCAAC AGCCCCCTCA GCATGGCGTT 
TATACCAATA GTTCTCCAAT GCCATCATCA AACACAGGTG TCATTTCTGC GGGCGTTTCG 
GTGCCTGTCC AGATTTCAAC GCAAAATGTA TCTGACCTAA CGGGAAGCAA TTACTGGCCA 
CGTCTTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

toy ( NM_001272151.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259080.1 Drosophila melanogaster twin of eyeless (toy), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00320
Clone ID Related Accession (Same CDS sequence)	NM_001272151.1
Accession Version	NM_001272151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twin of eyeless, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCTAA CAACTGAACA CATAATGCAT GGGCATCCCC ACTCGTCAGT CGGGCAGAGT 
ACTCTATTTG GGTGCTCCAC GGCGGGCCAT AGCGGAATAA ATCAGCTGGG CGGCGTATAT 
GTTAATGGCC GGCCACTGCC CGATTCAACG CGTCAAAAAA TTGTCGAATT GGCTCATTCC 
GGCGCACGTC CTTGTGATAT TTCAAGAATA CTACAAGTGT CCAACGGTTG CGTAAGCAAA 
ATTTTGGGCA GATATTATGA AACTGGATCG ATAAAACCTC GAGCTATAGG TGGTTCAAAG 
CCACGAGTAG CTACAACCCC GGTTGTGCAA AAAATTGCAG ATTACAAACG GGAATGTCCC 
AGCATATTTG CGTGGGAAAT ACGAGATCGA CTGCTATCGG AACAAGTTTG CAATAGTGAT 
AACATTCCAA GTGTTTCATC TATTAATCGA GTCTTACGTA ACCTGGCCTC ACAAAAGGAG 
CAGCAAGCTC AGCAACAAAA CGAATCCGTT TATGAAAAGC TTCGCATGTT TAATGGCCAA 
ACGGGCGGAT GGGCATGGTA TCCAAGCAAT ACAACGACGG CACATTTGAC GCTACCACCA 
GCAGCTTCCG TTGTGACATC TCCTGCAAAT TTATCAGGAC AGGCCGATCG GGATGATGTT 
CAAAAAAGAG AATTACAATT TTCAGTAGAA GTTTCGCATA CAAACTCTCA CGATAGTACA 
TCGGATGGAA ACTCTGAACA TAATTCATCC GGGGACGAAG ACTCTCAAAT GCGGTTGCGC 
CTAAAAAGGA AGTTACAGCG CAATCGGACA TCATTTTCTA ATGAGCAAAT TGACAGTCTT 
GAAAAAGAAT TTGAAAGAAC ACATTATCCC GATGTTTTTG CGCGAGAAAG GCTTGCTGAT 
AAAATTGGTT TGCCAGAGGT TTGGTTTTCA AACCGACGAG CTAAATGGCG CCGAGAAGAA 
AAAATGCGAA CTCAGAGACG ATCGGCCGAT ACCGTGGACG GCAGTGGTCG AACCAGCACG 
GCAAATAATC CTTCAGGAAC AACTGCATCT TCCTCCGTCG CAACGTCAAA CAACTCGACT 
CCAGGGATTG TGAACTCAGC AATCAACGTT GCGGAACGAA CATCATCTGC ATTAGTTTTG 
GGTGGTGAAG CTAATACTAC ACACACCAGC TCTGAAAGCC CACCCCTTCA GCCAGCGGCA 
CCGCGGCTAC CCTTAAATTC TGGATTCAAC ACCATGTACT CATCTATTCC ACAACCGATT 
GCAACGATGG CTGAAAATTA CAACTCCTCA TTAGGATCAA TGACCCCGTC ATGCTTACAA 
CAACGCGATG CCTATCCTTA CATGTTTCAC GATCCGTTAT CACTAGGATC TCCCTATGTG 
TCAGCCCACC ATCGAAACAC AGCTTGCAAC CCCTCAGCTG CGCACCAACA GCCCCCTCAG 
CATGGCGTTT ATACCAATAG TTCTCCAATG CCATCATCAA ACACAGGTGT CATTTCTGCG 
GGCGTTTCGG TGCCTGTCCA GATTTCAACG CAAAATGTAT CTGACCTAAC GGGAAGCAAT 
TACTGGCCAC GTCTTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259080.1
CDS	528..2108
Translation	MMLTTEHIMHGHPHSSVGQSTLFGCSTAGHSGINQLGGVYVNGRPLPDSTRQKIVELAHSGARPCDISRILQVSNGCVSKILGRYYETGSIKPRAIGGSKPRVATTPVVQKIADYKRECPSIFAWEIRDRLLSEQVCNSDNIPSVSSINRVLRNLASQKEQQAQQQNESVYEKLRMFNGQTGGWAWYPSNTTTAHLTLPPAASVVTSPANLSGQADRDDVQKRELQFSVEVSHTNSHDSTSDGNSEHNSSGDEDSQMRLRLKRKLQRNRTSFSNEQIDSLEKEFERTHYPDVFARERLADKIGLPEVWFSNRRAKWRREEKMRTQRRSADTVDGSGRTSTANNPSGTTASSSVATSNNSTPGIVNSAINVAERTSSALVLGGEANTTHTSSESPPLQPAAPRLPLNSGFNTMYSSIPQPIATMAENYNSSLGSMTPSCLQQRDAYPYMFHDPLSLGSPYVSAHHRNTACNPSAAHQQPPQHGVYTNSSPMPSSNTGVISAGVSVPVQISTQNVSDLTGSNYWPRLQ

Target ORF information:

RefSeq Version	NM_001272151.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twin of eyeless (toy), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272151.1

ORF Insert Sequence:

ATGATGCTAA CAACTGAACA CATAATGCAT GGGCATCCCC ACTCGTCAGT CGGGCAGAGT 
ACTCTATTTG GGTGCTCCAC GGCGGGCCAT AGCGGAATAA ATCAGCTGGG CGGCGTATAT 
GTTAATGGCC GGCCACTGCC CGATTCAACG CGTCAAAAAA TTGTCGAATT GGCTCATTCC 
GGCGCACGTC CTTGTGATAT TTCAAGAATA CTACAAGTGT CCAACGGTTG CGTAAGCAAA 
ATTTTGGGCA GATATTATGA AACTGGATCG ATAAAACCTC GAGCTATAGG TGGTTCAAAG 
CCACGAGTAG CTACAACCCC GGTTGTGCAA AAAATTGCAG ATTACAAACG GGAATGTCCC 
AGCATATTTG CGTGGGAAAT ACGAGATCGA CTGCTATCGG AACAAGTTTG CAATAGTGAT 
AACATTCCAA GTGTTTCATC TATTAATCGA GTCTTACGTA ACCTGGCCTC ACAAAAGGAG 
CAGCAAGCTC AGCAACAAAA CGAATCCGTT TATGAAAAGC TTCGCATGTT TAATGGCCAA 
ACGGGCGGAT GGGCATGGTA TCCAAGCAAT ACAACGACGG CACATTTGAC GCTACCACCA 
GCAGCTTCCG TTGTGACATC TCCTGCAAAT TTATCAGGAC AGGCCGATCG GGATGATGTT 
CAAAAAAGAG AATTACAATT TTCAGTAGAA GTTTCGCATA CAAACTCTCA CGATAGTACA 
TCGGATGGAA ACTCTGAACA TAATTCATCC GGGGACGAAG ACTCTCAAAT GCGGTTGCGC 
CTAAAAAGGA AGTTACAGCG CAATCGGACA TCATTTTCTA ATGAGCAAAT TGACAGTCTT 
GAAAAAGAAT TTGAAAGAAC ACATTATCCC GATGTTTTTG CGCGAGAAAG GCTTGCTGAT 
AAAATTGGTT TGCCAGAGGT TTGGTTTTCA AACCGACGAG CTAAATGGCG CCGAGAAGAA 
AAAATGCGAA CTCAGAGACG ATCGGCCGAT ACCGTGGACG GCAGTGGTCG AACCAGCACG 
GCAAATAATC CTTCAGGAAC AACTGCATCT TCCTCCGTCG CAACGTCAAA CAACTCGACT 
CCAGGGATTG TGAACTCAGC AATCAACGTT GCGGAACGAA CATCATCTGC ATTAGTTTTG 
GGTGGTGAAG CTAATACTAC ACACACCAGC TCTGAAAGCC CACCCCTTCA GCCAGCGGCA 
CCGCGGCTAC CCTTAAATTC TGGATTCAAC ACCATGTACT CATCTATTCC ACAACCGATT 
GCAACGATGG CTGAAAATTA CAACTCCTCA TTAGGATCAA TGACCCCGTC ATGCTTACAA 
CAACGCGATG CCTATCCTTA CATGTTTCAC GATCCGTTAT CACTAGGATC TCCCTATGTG 
TCAGCCCACC ATCGAAACAC AGCTTGCAAC CCCTCAGCTG CGCACCAACA GCCCCCTCAG 
CATGGCGTTT ATACCAATAG TTCTCCAATG CCATCATCAA ACACAGGTGT CATTTCTGCG 
GGCGTTTCGG TGCCTGTCCA GATTTCAACG CAAAATGTAT CTGACCTAAC GGGAAGCAAT 
TACTGGCCAC GTCTTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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toy cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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