gw cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following gw gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gw cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa00286 NM_166783.2
Latest version!
Drosophila melanogaster gawky (gw), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa00287 NM_001014691.2
Latest version!
Drosophila melanogaster gawky (gw), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa00288 NM_166780.2
Latest version!
Drosophila melanogaster gawky (gw), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa00288 NM_166784.2
Latest version!
Drosophila melanogaster gawky (gw), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa00288 NM_166785.2
Latest version!
Drosophila melanogaster gawky (gw), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa00288 NM_166781.2
Latest version!
Drosophila melanogaster gawky (gw), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa00286
    Clone ID Related Accession (Same CDS sequence) NM_166783.2
    Accession Version NM_166783.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product gawky, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166783.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGATCAAA AGCATAGAAG ATGATCCCAG CATTACACCA
    GGAAGCGTTG CTAGATCTCC ATTGTCTATT AATTCGACGC CAAAAGATGC TGACATATTT
    GCCAATACCG GTAAAAATTC ACCGACTGAT TTACCGCCAC TAAGTTTATC GTCGTCTACA
    TGGAGTTTTA ATCCAAACCA AAATTATCCG AGTCACAGTT GGTCTGACAA TAGTCAACAA
    TGTACCGCCA CTTCGGAGCT TTGGACAAGC CCGCTAAATA AATCATCGTC TCGAGGTCCC
    CCGCCAGGAT TGACTGCCAA TTCAAATAAA TCTGCAAATA GTAATGCGTC AACGCCAACA
    ACTATTACCG GAGGTGCGAA TGGATGGTTA CAGCCTCGAA GTGGCGGTGT TCAAACCACA
    AACACTAATT GGACAGGTGG TAACACCACT TGGGGCTCCA GTTGGTTGCT TTTGAAAAAT
    CTAACAGCAC AGATTGATGG TCCTACTTTG CGTACACTGT GTATGCAGCA TGGGCCCCTT
    GTCAGCTTTC ACCCGTATTT GAACCAAGGA ATTGCCTTAT GTAAATATAC TACTCGTGAG
    GAGGCGAACA AGGCGCAAAT GGCGTTAAAC AACTGTGTCC TCGCCAACAC CACAATATTT
    GCTGAATCTC CCAGCGAGAA CGAGGTGCAA AGCATTATGC AGCACTTACC ACAAACTCCT
    TCCTCTACAA GCTCTAGTGG AACTAGTGGT GGCAACGTCG GAGGCGTCGG CACTTCAGCC
    AATAATGCAA ACAGTGGTTC TGCAGCTTGT CTGTCCGGAA ACAATAGCGG CAACGGAAAC
    GGCAGCGCGA GCGGCGCCGG CAGCGGCAAC AATGGCAACA GTAGCTGCAA CAACAGTGCC
    GCCGGGGGGG GCAGCAGCAG CAACAACACG ATTACCACTG TAGCAAATTC GAATCTTGTT
    GGTTCTAGTG GCTCTGTCTC AAATTCCTCT GGCGTTACTG CTAACTCTAG TACTGTTTCT
    GTAGTTAGTT GTACAGCGAG TGGGAATTCC ATAAATGGGG CAGGTACTGC AAACAGTTCT
    GGTTCAAAGA GTAGTGCAAA CAATTTAGCT AGCGGCCAGT CTAGCGCTTC TAACTTAACT
    AATAGCACCA ATTCAACATG GCGACAAACT AGCCAAAACC AAGCTCTTCA AAGTCAAAGC
    AGGCCATCAG GCAGAGAAGC TGACTTTGAT TATATATCTC TCGTTTATTC CATTGTTGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_726599.2
    CDS1446..5591
    Translation

    Target ORF information:

    RefSeq Version NM_166783.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster gawky (gw), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_166783.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGATCAAA AGCATAGAAG ATGATCCCAG CATTACACCA
    GGAAGCGTTG CTAGATCTCC ATTGTCTATT AATTCGACGC CAAAAGATGC TGACATATTT
    GCCAATACCG GTAAAAATTC ACCGACTGAT TTACCGCCAC TAAGTTTATC GTCGTCTACA
    TGGAGTTTTA ATCCAAACCA AAATTATCCG AGTCACAGTT GGTCTGACAA TAGTCAACAA
    TGTACCGCCA CTTCGGAGCT TTGGACAAGC CCGCTAAATA AATCATCGTC TCGAGGTCCC
    CCGCCAGGAT TGACTGCCAA TTCAAATAAA TCTGCAAATA GTAATGCGTC AACGCCAACA
    ACTATTACCG GAGGTGCGAA TGGATGGTTA CAGCCTCGAA GTGGCGGTGT TCAAACCACA
    AACACTAATT GGACAGGTGG TAACACCACT TGGGGCTCCA GTTGGTTGCT TTTGAAAAAT
    CTAACAGCAC AGATTGATGG TCCTACTTTG CGTACACTGT GTATGCAGCA TGGGCCCCTT
    GTCAGCTTTC ACCCGTATTT GAACCAAGGA ATTGCCTTAT GTAAATATAC TACTCGTGAG
    GAGGCGAACA AGGCGCAAAT GGCGTTAAAC AACTGTGTCC TCGCCAACAC CACAATATTT
    GCTGAATCTC CCAGCGAGAA CGAGGTGCAA AGCATTATGC AGCACTTACC ACAAACTCCT
    TCCTCTACAA GCTCTAGTGG AACTAGTGGT GGCAACGTCG GAGGCGTCGG CACTTCAGCC
    AATAATGCAA ACAGTGGTTC TGCAGCTTGT CTGTCCGGAA ACAATAGCGG CAACGGAAAC
    GGCAGCGCGA GCGGCGCCGG CAGCGGCAAC AATGGCAACA GTAGCTGCAA CAACAGTGCC
    GCCGGGGGGG GCAGCAGCAG CAACAACACG ATTACCACTG TAGCAAATTC GAATCTTGTT
    GGTTCTAGTG GCTCTGTCTC AAATTCCTCT GGCGTTACTG CTAACTCTAG TACTGTTTCT
    GTAGTTAGTT GTACAGCGAG TGGGAATTCC ATAAATGGGG CAGGTACTGC AAACAGTTCT
    GGTTCAAAGA GTAGTGCAAA CAATTTAGCT AGCGGCCAGT CTAGCGCTTC TAACTTAACT
    AATAGCACCA ATTCAACATG GCGACAAACT AGCCAAAACC AAGCTCTTCA AAGTCAAAGC
    AGGCCATCAG GCAGAGAAGC TGACTTTGAT TATATATCTC TCGTTTATTC CATTGTTGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00287
    Clone ID Related Accession (Same CDS sequence) NM_001014691.2
    Accession Version NM_001014691.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product gawky, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001014691.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC
    ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT
    GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG
    TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTTGGTCTGA CAATAGTCAA
    CAATGTACCG CCACTTCGGA GCTTTGGACA AGCCCGCTAA ATAAATCATC GTCTCGAGGT
    CCCCCGCCAG GATTGACTGC CAATTCAAAT AAATCTGCAA ATAGTAATGC GTCAACGCCA
    ACAACTATTA CCGGAGGTGC GAATGGATGG TTACAGCCTC GAAGTGGCGG TGTTCAAACC
    ACAAACACTA ATTGGACAGG TGGTAACACC ACTTGGGGCT CCAGTTGGTT GCTTTTGAAA
    AATCTAACAG CACAGATTGA TGGTCCTACT TTGCGTACAC TGTGTATGCA GCATGGGCCC
    CTTGTCAGCT TTCACCCGTA TTTGAACCAA GGAATTGCCT TATGTAAATA TACTACTCGT
    GAGGAGGCGA ACAAGGCGCA AATGGCGTTA AACAACTGTG TCCTCGCCAA CACCACAATA
    TTTGCTGAAT CTCCCAGCGA GAACGAGGTG CAAAGCATTA TGCAGCACTT ACCACAAACT
    CCTTCCTCTA CAAGCTCTAG TGGAACTAGT GGTGGCAACG TCGGAGGCGT CGGCACTTCA
    GCCAATAATG CAAACAGTGG TTCTGCAGCT TGTCTGTCCG GAAACAATAG CGGCAACGGA
    AACGGCAGCG CGAGCGGCGC CGGCAGCGGC AACAATGGCA ACAGTAGCTG CAACAACAGT
    GCCGCCGGGG GGGGCAGCAG CAGCAACAAC ACGATTACCA CTGTAGCAAA TTCGAATCTT
    GTTGGTTCTA GTGGCTCTGT CTCAAATTCC TCTGGCGTTA CTGCTAACTC TAGTACTGTT
    TCTGTAGTTA GTTGTACAGC GAGTGGGAAT TCCATAAATG GGGCAGGTAC TGCAAACAGT
    TCTGGTTCAA AGAGTAGTGC AAACAATTTA GCTAGCGGCC AGTCTAGCGC TTCTAACTTA
    ACTAATAGCA CCAATTCAAC ATGGCGACAA ACTAGCCAAA ACCAAGCTCT TCAAAGTCAA
    AGCAGGCCAT CAGGCAGAGA AGCTGACTTT GATTATATAT CTCTCGTTTA TTCCATTGTT
    GATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001014691.2
    CDS352..4500
    Translation

    Target ORF information:

    RefSeq Version NM_001014691.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster gawky (gw), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001014691.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC
    ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT
    GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG
    TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTTGGTCTGA CAATAGTCAA
    CAATGTACCG CCACTTCGGA GCTTTGGACA AGCCCGCTAA ATAAATCATC GTCTCGAGGT
    CCCCCGCCAG GATTGACTGC CAATTCAAAT AAATCTGCAA ATAGTAATGC GTCAACGCCA
    ACAACTATTA CCGGAGGTGC GAATGGATGG TTACAGCCTC GAAGTGGCGG TGTTCAAACC
    ACAAACACTA ATTGGACAGG TGGTAACACC ACTTGGGGCT CCAGTTGGTT GCTTTTGAAA
    AATCTAACAG CACAGATTGA TGGTCCTACT TTGCGTACAC TGTGTATGCA GCATGGGCCC
    CTTGTCAGCT TTCACCCGTA TTTGAACCAA GGAATTGCCT TATGTAAATA TACTACTCGT
    GAGGAGGCGA ACAAGGCGCA AATGGCGTTA AACAACTGTG TCCTCGCCAA CACCACAATA
    TTTGCTGAAT CTCCCAGCGA GAACGAGGTG CAAAGCATTA TGCAGCACTT ACCACAAACT
    CCTTCCTCTA CAAGCTCTAG TGGAACTAGT GGTGGCAACG TCGGAGGCGT CGGCACTTCA
    GCCAATAATG CAAACAGTGG TTCTGCAGCT TGTCTGTCCG GAAACAATAG CGGCAACGGA
    AACGGCAGCG CGAGCGGCGC CGGCAGCGGC AACAATGGCA ACAGTAGCTG CAACAACAGT
    GCCGCCGGGG GGGGCAGCAG CAGCAACAAC ACGATTACCA CTGTAGCAAA TTCGAATCTT
    GTTGGTTCTA GTGGCTCTGT CTCAAATTCC TCTGGCGTTA CTGCTAACTC TAGTACTGTT
    TCTGTAGTTA GTTGTACAGC GAGTGGGAAT TCCATAAATG GGGCAGGTAC TGCAAACAGT
    TCTGGTTCAA AGAGTAGTGC AAACAATTTA GCTAGCGGCC AGTCTAGCGC TTCTAACTTA
    ACTAATAGCA CCAATTCAAC ATGGCGACAA ACTAGCCAAA ACCAAGCTCT TCAAAGTCAA
    AGCAGGCCAT CAGGCAGAGA AGCTGACTTT GATTATATAT CTCTCGTTTA TTCCATTGTT
    GATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00288
    Clone ID Related Accession (Same CDS sequence) NM_166784.2 , NM_166781.2 , NM_166780.2 , NM_166785.2
    Accession Version NM_166784.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product gawky, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166784.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC
    ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT
    GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG
    TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT
    AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT
    CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA
    ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT
    CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT
    TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT
    GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT
    ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC
    ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA
    CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC
    ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC
    AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC
    AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG
    AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT
    ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA
    AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT
    AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA
    AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC
    ATTGTTGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_726600.1
    CDS235..4389
    Translation

    Target ORF information:

    RefSeq Version NM_166784.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster gawky (gw), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_166784.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC
    ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT
    GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG
    TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT
    AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT
    CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA
    ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT
    CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT
    TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT
    GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT
    ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC
    ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA
    CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC
    ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC
    AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC
    AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG
    AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT
    ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA
    AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT
    AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA
    AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC
    ATTGTTGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00288
    Clone ID Related Accession (Same CDS sequence) NM_166784.2 , NM_166781.2 , NM_166780.2 , NM_166785.2
    Accession Version NM_166781.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product gawky, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166781.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC
    ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT
    GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG
    TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT
    AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT
    CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA
    ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT
    CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT
    TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT
    GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT
    ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC
    ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA
    CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC
    ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC
    AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC
    AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG
    AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT
    ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA
    AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT
    AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA
    AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC
    ATTGTTGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_726597.1
    CDS352..4506
    Translation

    Target ORF information:

    RefSeq Version NM_166781.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster gawky (gw), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_166781.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC
    ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT
    GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG
    TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT
    AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT
    CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA
    ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT
    CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT
    TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT
    GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT
    ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC
    ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA
    CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC
    ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC
    AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC
    AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG
    AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT
    ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA
    AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT
    AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA
    AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC
    ATTGTTGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00288
    Clone ID Related Accession (Same CDS sequence) NM_166784.2 , NM_166781.2 , NM_166780.2 , NM_166785.2
    Accession Version NM_166780.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product gawky, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166780.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC
    ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT
    GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG
    TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT
    AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT
    CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA
    ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT
    CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT
    TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT
    GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT
    ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC
    ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA
    CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC
    ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC
    AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC
    AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG
    AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT
    ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA
    AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT
    AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA
    AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC
    ATTGTTGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_726596.1
    CDS596..4750
    Translation

    Target ORF information:

    RefSeq Version NM_166780.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster gawky (gw), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_166780.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC
    ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT
    GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG
    TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT
    AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT
    CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA
    ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT
    CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT
    TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT
    GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT
    ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC
    ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA
    CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC
    ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC
    AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC
    AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG
    AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT
    ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA
    AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT
    AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA
    AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC
    ATTGTTGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00288
    Clone ID Related Accession (Same CDS sequence) NM_166784.2 , NM_166781.2 , NM_166780.2 , NM_166785.2
    Accession Version NM_166785.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product gawky, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166785.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC
    ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT
    GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG
    TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT
    AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT
    CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA
    ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT
    CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT
    TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT
    GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT
    ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC
    ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA
    CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC
    ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC
    AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC
    AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG
    AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT
    ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA
    AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT
    AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA
    AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC
    ATTGTTGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_726601.1
    CDS278..4432
    Translation

    Target ORF information:

    RefSeq Version NM_166785.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster gawky (gw), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_166785.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
    AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG
    TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT
    CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA
    GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA
    GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC
    GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC
    GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA
    ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA
    CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC
    GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT
    ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT
    CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT
    GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC
    GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC
    GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA
    CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG
    CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT
    AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA
    AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT
    CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG
    GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT
    GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG
    GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT
    GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG
    AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG
    GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG
    AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC
    GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG
    GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT
    GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT
    CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT
    AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG
    GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT
    AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA
    GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC
    ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG
    ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG
    CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG
    AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA
    CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC
    CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT
    CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT
    GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA
    GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA
    AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG
    TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA
    GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG
    TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC
    ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT
    GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG
    TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT
    AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT
    CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA
    ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT
    CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT
    TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT
    GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT
    ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC
    ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA
    CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC
    ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC
    AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC
    AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG
    AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT
    ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA
    AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT
    AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA
    AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC
    ATTGTTGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.