gw cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gw
Entrez Gene ID	43808
Full Name	gawky
Synonyms	CG11484,CG31992,CG9905,Dmel\CG31992,GQ182,GW,GW182,Gw,Gw182,anon-EST:Posey205,dGW182,gw182
General protein information	Preferred Names gawky Names CG31992 gene product from transcript CG31992-RI CG31992-PA CG31992-PB CG31992-PE CG31992-PF CG31992-PI CG31992-PJ gawki gw-PA gw-PB gw-PE gw-PF gw-PI gw-PJ
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102D2-102D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_166784.2	NP_726600.1	gawky, isoform E
NM_001014691.2	NP_001014691.2	gawky, isoform J
NM_166781.2	NP_726597.1	gawky, isoform B
NM_166780.2	NP_726596.1	gawky, isoform A
NM_166783.2	NP_726599.2	gawky, isoform I
NM_166785.2	NP_726601.1	gawky, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	gw	NP_726601.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007802	XP_317704.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonistryed that GW182's gene silencing activity functions as a rheostat for PDFR signaling and thus profoundly impacts the circadian neural network and its response to environmental inputs.
Title: GW182 controls Drosophila circadian behavior and PDF-receptor signaling.

miRNA-mediated translational repression and degradation are mechanistically linked and are triggered through the interactions of GW182 with poly(A)-binding protein and deadenylases.
Title: The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets.

Gawky plays a critical role in miRNA-mediated gene silencing. (Review)
Title: Gawky (GW) is the Drosophila melanogaster GW182 homologue.

MicroRNAs utilize at least three distinct silencing pathways: GW182-mediated deadenylation and GW182-dependent and -independent repression of early translation initiation.
Title: MicroRNAs mediate gene silencing via multiple different pathways in drosophila.

Data demonstrate that in human and Drosophila melanogaster cells, the critical repressive features of both the N-terminal and C-terminal effector domains of GW182 proteins are Gly/Ser/Thr-Trp (G/S/TW) or Trp-Gly/Ser/Thr (WG/S/T) motifs.
Title: miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs.

The following gw gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gw cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00286	NM_166783.2 Latest version!	Drosophila melanogaster gawky (gw), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa00287	NM_001014691.2 Latest version!	Drosophila melanogaster gawky (gw), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa00288	NM_166780.2 Latest version!	Drosophila melanogaster gawky (gw), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa00288	NM_166784.2 Latest version!	Drosophila melanogaster gawky (gw), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa00288	NM_166785.2 Latest version!	Drosophila melanogaster gawky (gw), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa00288	NM_166781.2 Latest version!	Drosophila melanogaster gawky (gw), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gw ( NM_166783.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726599.2 Drosophila melanogaster gawky (gw), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa00286
Clone ID Related Accession (Same CDS sequence)	NM_166783.2
Accession Version	NM_166783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gawky, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166783.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGATCAAA AGCATAGAAG ATGATCCCAG CATTACACCA 
GGAAGCGTTG CTAGATCTCC ATTGTCTATT AATTCGACGC CAAAAGATGC TGACATATTT 
GCCAATACCG GTAAAAATTC ACCGACTGAT TTACCGCCAC TAAGTTTATC GTCGTCTACA 
TGGAGTTTTA ATCCAAACCA AAATTATCCG AGTCACAGTT GGTCTGACAA TAGTCAACAA 
TGTACCGCCA CTTCGGAGCT TTGGACAAGC CCGCTAAATA AATCATCGTC TCGAGGTCCC 
CCGCCAGGAT TGACTGCCAA TTCAAATAAA TCTGCAAATA GTAATGCGTC AACGCCAACA 
ACTATTACCG GAGGTGCGAA TGGATGGTTA CAGCCTCGAA GTGGCGGTGT TCAAACCACA 
AACACTAATT GGACAGGTGG TAACACCACT TGGGGCTCCA GTTGGTTGCT TTTGAAAAAT 
CTAACAGCAC AGATTGATGG TCCTACTTTG CGTACACTGT GTATGCAGCA TGGGCCCCTT 
GTCAGCTTTC ACCCGTATTT GAACCAAGGA ATTGCCTTAT GTAAATATAC TACTCGTGAG 
GAGGCGAACA AGGCGCAAAT GGCGTTAAAC AACTGTGTCC TCGCCAACAC CACAATATTT 
GCTGAATCTC CCAGCGAGAA CGAGGTGCAA AGCATTATGC AGCACTTACC ACAAACTCCT 
TCCTCTACAA GCTCTAGTGG AACTAGTGGT GGCAACGTCG GAGGCGTCGG CACTTCAGCC 
AATAATGCAA ACAGTGGTTC TGCAGCTTGT CTGTCCGGAA ACAATAGCGG CAACGGAAAC 
GGCAGCGCGA GCGGCGCCGG CAGCGGCAAC AATGGCAACA GTAGCTGCAA CAACAGTGCC 
GCCGGGGGGG GCAGCAGCAG CAACAACACG ATTACCACTG TAGCAAATTC GAATCTTGTT 
GGTTCTAGTG GCTCTGTCTC AAATTCCTCT GGCGTTACTG CTAACTCTAG TACTGTTTCT 
GTAGTTAGTT GTACAGCGAG TGGGAATTCC ATAAATGGGG CAGGTACTGC AAACAGTTCT 
GGTTCAAAGA GTAGTGCAAA CAATTTAGCT AGCGGCCAGT CTAGCGCTTC TAACTTAACT 
AATAGCACCA ATTCAACATG GCGACAAACT AGCCAAAACC AAGCTCTTCA AAGTCAAAGC 
AGGCCATCAG GCAGAGAAGC TGACTTTGAT TATATATCTC TCGTTTATTC CATTGTTGAT 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726599.2
CDS	1446..5591
Translation	MREALFSQDGWGCQHVNQDTNWEVPSSPEPANKDAPGPPMWKPSINNGTDLWESNLRNGGQPAAQQVPKPSWGHTPSSNLGGTWGEDDDGADSSSVWTGGAVSNAGSGAAVGVNQAGVNVGPGGVVSSGGPQWGQGVVGVGLGSTGGNGSSNITGSSGVATGSSGNSSNAGNGWGDPREIRPLGVGGSMDIRNVEHRGGNGSGATSSDPRDIRMIDPRDPIRGDPRGISGRLNGTSEMWGHHPQMSHNQLQGINKMVGQSVATASTSVGTSGSGIGPGGPGPSTVSGNIPTQWGPAQPVSVGVSGPKDMSKQISGWEEPSPPPQRRSIPNYDDGTSLWGQQTRVPAASGHWKDMTDSIGRSSHLMRGQSQTGGIGIAGVGNSNVPVGANPSNPISSVVGPQARIPSVGGVQHKPDGGAMWVHSGNVGGRNNVAAVTTWGDDTHSVNVGAPSSGSVSSNNWVDDKSNSTLAQNSWSDPAPVGVSWGNKQSKPPSNSASSGWSTAAGVVDGVDLGSEWNTHGGIIGKSQQQQKLAGLNVGMVNVINAEIIKQSKQYRILVENGFKKEDVERALVIANMNIEEAADMLRANSSLSMDGWRRHDESLGSYADHNSSTSSGGFAGRYPVNSGQPSMSFPHNNLMNNMGGTAVTGGNNNTNMTALQVQKYLNQGQHGVAVGPQAVGNSSAVSVGFGQNTSNAAVAGAASVNIAANTNNQPSGQQIRMLGQQIQLAIHSGFISSQILTQPLTQTTLNLLNQLLSNIKHLQAAQQSLTRGGNVNPMAVNVAISKYKQQIQNLQNQINAQQAVYVKQQNMQPTSQQQQPQQQQLPSVHLSNSGNDYLRGHDAINNLQSNFSELNINKPSGYQGASNQQSRLNQWKLPVLDKEINSDSTEFSRAPGATKQNLTANTSNINSLGLQNDSTWSTGRSIGDGWPDPSSDNENKDWSVAQPTSAATAYTDLVQEFEPGKPWKIKSIEDDPSITPGSVARSPLSINSTPKDADIFANTGKNSPTDLPPLSLSSSTWSFNPNQNYPSHSWSDNSQQCTATSELWTSPLNKSSSRGPPPGLTANSNKSANSNASTPTTITGGANGWLQPRSGGVQTTNTNWTGGNTTWGSSWLLLKNLTAQIDGPTLRTLCMQHGPLVSFHPYLNQGIALCKYTTREEANKAQMALNNCVLANTTIFAESPSENEVQSIMQHLPQTPSSTSSSGTSGGNVGGVGTSANNANSGSAACLSGNNSGNGNGSASGAGSGNNGNSSCNNSAAGGGSSSNNTITTVANSNLVGSSGSVSNSSGVTANSSTVSVVSCTASGNSINGAGTANSSGSKSSANNLASGQSSASNLTNSTNSTWRQTSQNQALQSQSRPSGREADFDYISLVYSIVDD

Target ORF information:

RefSeq Version	NM_166783.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gawky (gw), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166783.2

ORF Insert Sequence:

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGATCAAA AGCATAGAAG ATGATCCCAG CATTACACCA 
GGAAGCGTTG CTAGATCTCC ATTGTCTATT AATTCGACGC CAAAAGATGC TGACATATTT 
GCCAATACCG GTAAAAATTC ACCGACTGAT TTACCGCCAC TAAGTTTATC GTCGTCTACA 
TGGAGTTTTA ATCCAAACCA AAATTATCCG AGTCACAGTT GGTCTGACAA TAGTCAACAA 
TGTACCGCCA CTTCGGAGCT TTGGACAAGC CCGCTAAATA AATCATCGTC TCGAGGTCCC 
CCGCCAGGAT TGACTGCCAA TTCAAATAAA TCTGCAAATA GTAATGCGTC AACGCCAACA 
ACTATTACCG GAGGTGCGAA TGGATGGTTA CAGCCTCGAA GTGGCGGTGT TCAAACCACA 
AACACTAATT GGACAGGTGG TAACACCACT TGGGGCTCCA GTTGGTTGCT TTTGAAAAAT 
CTAACAGCAC AGATTGATGG TCCTACTTTG CGTACACTGT GTATGCAGCA TGGGCCCCTT 
GTCAGCTTTC ACCCGTATTT GAACCAAGGA ATTGCCTTAT GTAAATATAC TACTCGTGAG 
GAGGCGAACA AGGCGCAAAT GGCGTTAAAC AACTGTGTCC TCGCCAACAC CACAATATTT 
GCTGAATCTC CCAGCGAGAA CGAGGTGCAA AGCATTATGC AGCACTTACC ACAAACTCCT 
TCCTCTACAA GCTCTAGTGG AACTAGTGGT GGCAACGTCG GAGGCGTCGG CACTTCAGCC 
AATAATGCAA ACAGTGGTTC TGCAGCTTGT CTGTCCGGAA ACAATAGCGG CAACGGAAAC 
GGCAGCGCGA GCGGCGCCGG CAGCGGCAAC AATGGCAACA GTAGCTGCAA CAACAGTGCC 
GCCGGGGGGG GCAGCAGCAG CAACAACACG ATTACCACTG TAGCAAATTC GAATCTTGTT 
GGTTCTAGTG GCTCTGTCTC AAATTCCTCT GGCGTTACTG CTAACTCTAG TACTGTTTCT 
GTAGTTAGTT GTACAGCGAG TGGGAATTCC ATAAATGGGG CAGGTACTGC AAACAGTTCT 
GGTTCAAAGA GTAGTGCAAA CAATTTAGCT AGCGGCCAGT CTAGCGCTTC TAACTTAACT 
AATAGCACCA ATTCAACATG GCGACAAACT AGCCAAAACC AAGCTCTTCA AAGTCAAAGC 
AGGCCATCAG GCAGAGAAGC TGACTTTGAT TATATATCTC TCGTTTATTC CATTGTTGAT 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gw ( NM_001014691.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014691.2 Drosophila melanogaster gawky (gw), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa00287
Clone ID Related Accession (Same CDS sequence)	NM_001014691.2
Accession Version	NM_001014691.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gawky, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001014691.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC 
ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT 
GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG 
TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTTGGTCTGA CAATAGTCAA 
CAATGTACCG CCACTTCGGA GCTTTGGACA AGCCCGCTAA ATAAATCATC GTCTCGAGGT 
CCCCCGCCAG GATTGACTGC CAATTCAAAT AAATCTGCAA ATAGTAATGC GTCAACGCCA 
ACAACTATTA CCGGAGGTGC GAATGGATGG TTACAGCCTC GAAGTGGCGG TGTTCAAACC 
ACAAACACTA ATTGGACAGG TGGTAACACC ACTTGGGGCT CCAGTTGGTT GCTTTTGAAA 
AATCTAACAG CACAGATTGA TGGTCCTACT TTGCGTACAC TGTGTATGCA GCATGGGCCC 
CTTGTCAGCT TTCACCCGTA TTTGAACCAA GGAATTGCCT TATGTAAATA TACTACTCGT 
GAGGAGGCGA ACAAGGCGCA AATGGCGTTA AACAACTGTG TCCTCGCCAA CACCACAATA 
TTTGCTGAAT CTCCCAGCGA GAACGAGGTG CAAAGCATTA TGCAGCACTT ACCACAAACT 
CCTTCCTCTA CAAGCTCTAG TGGAACTAGT GGTGGCAACG TCGGAGGCGT CGGCACTTCA 
GCCAATAATG CAAACAGTGG TTCTGCAGCT TGTCTGTCCG GAAACAATAG CGGCAACGGA 
AACGGCAGCG CGAGCGGCGC CGGCAGCGGC AACAATGGCA ACAGTAGCTG CAACAACAGT 
GCCGCCGGGG GGGGCAGCAG CAGCAACAAC ACGATTACCA CTGTAGCAAA TTCGAATCTT 
GTTGGTTCTA GTGGCTCTGT CTCAAATTCC TCTGGCGTTA CTGCTAACTC TAGTACTGTT 
TCTGTAGTTA GTTGTACAGC GAGTGGGAAT TCCATAAATG GGGCAGGTAC TGCAAACAGT 
TCTGGTTCAA AGAGTAGTGC AAACAATTTA GCTAGCGGCC AGTCTAGCGC TTCTAACTTA 
ACTAATAGCA CCAATTCAAC ATGGCGACAA ACTAGCCAAA ACCAAGCTCT TCAAAGTCAA 
AGCAGGCCAT CAGGCAGAGA AGCTGACTTT GATTATATAT CTCTCGTTTA TTCCATTGTT 
GATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014691.2
CDS	352..4500
Translation	MREALFSQDGWGCQHVNQDTNWEVPSSPEPANKDAPGPPMWKPSINNGTDLWESNLRNGGQPAAQQVPKPSWGHTPSSNLGGTWGEDDDGADSSSVWTGGAVSNAGSGAAVGVNQAGVNVGPGGVVSSGGPQWGQGVVGVGLGSTGGNGSSNITGSSGVATGSSGNSSNAGNGWGDPREIRPLGVGGSMDIRNVEHRGGNGSGATSSDPRDIRMIDPRDPIRGDPRGISGRLNGTSEMWGHHPQMSHNQLQGINKMVGQSVATASTSVGTSGSGIGPGGPGPSTVSGNIPTQWGPAQPVSVGVSGPKDMSKQISGWEEPSPPPQRRSIPNYDDGTSLWGQQTRVPAASGHWKDMTDSIGRSSHLMRGQSQTGGIGIAGVGNSNVPVGANPSNPISSVVGPQARIPSVGGVQHKPDGGAMWVHSGNVGGRNNVAAVTTWGDDTHSVNVGAPSSGSVSSNNWVDDKSNSTLAQNSWSDPAPVGVSWGNKQSKPPSNSASSGWSTAAGVVDGVDLGSEWNTHGGIIGKSQQQQKLAGLNVGMVNVINAEIIKQSKQYRILVENGFKKEDVERALVIANMNIEEAADMLRANSSLSMDGWRRHDESLGSYADHNSSTSSGGFAGRYPVNSGQPSMSFPHNNLMNNMGGTAVTGGNNNTNMTALQVQKYLNQGQHGVAVGPQAVGNSSAVSVGFGQNTSNAAVAGAASVNIAANTNNQPSGQQIRMLGQQIQLAIHSGFISSQILTQPLTQTTLNLLNQLLSNIKHLQAAQQSLTRGGNVNPMAVNVAISKYKQQIQNLQNQINAQQAVYVKQQNMQPTSQQQQPQQQQLPSVHLSNSGNDYLRGHDAINNLQSNFSELNINKPSGYQGASNQQSRLNQWKLPVLDKEINSDSTEFSRAPGATKQNLTANTSNINSLGLQNDSTWSTGRSIGDGWPDPSSDNENKDWSVAQPTSAATAYTDLVQEFEPGKPWKGSQIKSIEDDPSITPGSVARSPLSINSTPKDADIFANTGKNSPTDLPPLSLSSSTWSFNPNQNYPSWSDNSQQCTATSELWTSPLNKSSSRGPPPGLTANSNKSANSNASTPTTITGGANGWLQPRSGGVQTTNTNWTGGNTTWGSSWLLLKNLTAQIDGPTLRTLCMQHGPLVSFHPYLNQGIALCKYTTREEANKAQMALNNCVLANTTIFAESPSENEVQSIMQHLPQTPSSTSSSGTSGGNVGGVGTSANNANSGSAACLSGNNSGNGNGSASGAGSGNNGNSSCNNSAAGGGSSSNNTITTVANSNLVGSSGSVSNSSGVTANSSTVSVVSCTASGNSINGAGTANSSGSKSSANNLASGQSSASNLTNSTNSTWRQTSQNQALQSQSRPSGREADFDYISLVYSIVDD

Target ORF information:

RefSeq Version	NM_001014691.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gawky (gw), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014691.2

ORF Insert Sequence:

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC 
ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT 
GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG 
TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTTGGTCTGA CAATAGTCAA 
CAATGTACCG CCACTTCGGA GCTTTGGACA AGCCCGCTAA ATAAATCATC GTCTCGAGGT 
CCCCCGCCAG GATTGACTGC CAATTCAAAT AAATCTGCAA ATAGTAATGC GTCAACGCCA 
ACAACTATTA CCGGAGGTGC GAATGGATGG TTACAGCCTC GAAGTGGCGG TGTTCAAACC 
ACAAACACTA ATTGGACAGG TGGTAACACC ACTTGGGGCT CCAGTTGGTT GCTTTTGAAA 
AATCTAACAG CACAGATTGA TGGTCCTACT TTGCGTACAC TGTGTATGCA GCATGGGCCC 
CTTGTCAGCT TTCACCCGTA TTTGAACCAA GGAATTGCCT TATGTAAATA TACTACTCGT 
GAGGAGGCGA ACAAGGCGCA AATGGCGTTA AACAACTGTG TCCTCGCCAA CACCACAATA 
TTTGCTGAAT CTCCCAGCGA GAACGAGGTG CAAAGCATTA TGCAGCACTT ACCACAAACT 
CCTTCCTCTA CAAGCTCTAG TGGAACTAGT GGTGGCAACG TCGGAGGCGT CGGCACTTCA 
GCCAATAATG CAAACAGTGG TTCTGCAGCT TGTCTGTCCG GAAACAATAG CGGCAACGGA 
AACGGCAGCG CGAGCGGCGC CGGCAGCGGC AACAATGGCA ACAGTAGCTG CAACAACAGT 
GCCGCCGGGG GGGGCAGCAG CAGCAACAAC ACGATTACCA CTGTAGCAAA TTCGAATCTT 
GTTGGTTCTA GTGGCTCTGT CTCAAATTCC TCTGGCGTTA CTGCTAACTC TAGTACTGTT 
TCTGTAGTTA GTTGTACAGC GAGTGGGAAT TCCATAAATG GGGCAGGTAC TGCAAACAGT 
TCTGGTTCAA AGAGTAGTGC AAACAATTTA GCTAGCGGCC AGTCTAGCGC TTCTAACTTA 
ACTAATAGCA CCAATTCAAC ATGGCGACAA ACTAGCCAAA ACCAAGCTCT TCAAAGTCAA 
AGCAGGCCAT CAGGCAGAGA AGCTGACTTT GATTATATAT CTCTCGTTTA TTCCATTGTT 
GATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gw ( NM_166784.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726600.1 Drosophila melanogaster gawky (gw), transcript variant E, mRNA.

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CloneID	OFa00288
Clone ID Related Accession (Same CDS sequence)	NM_166784.2 , NM_166781.2 , NM_166780.2 , NM_166785.2
Accession Version	NM_166784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gawky, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166784.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC 
ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT 
GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG 
TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT 
AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT 
CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA 
ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT 
CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT 
TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT 
GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT 
ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC 
ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA 
CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC 
ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC 
AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC 
AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG 
AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT 
ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA 
AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT 
AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA 
AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC 
ATTGTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726600.1
CDS	235..4389
Translation	MREALFSQDGWGCQHVNQDTNWEVPSSPEPANKDAPGPPMWKPSINNGTDLWESNLRNGGQPAAQQVPKPSWGHTPSSNLGGTWGEDDDGADSSSVWTGGAVSNAGSGAAVGVNQAGVNVGPGGVVSSGGPQWGQGVVGVGLGSTGGNGSSNITGSSGVATGSSGNSSNAGNGWGDPREIRPLGVGGSMDIRNVEHRGGNGSGATSSDPRDIRMIDPRDPIRGDPRGISGRLNGTSEMWGHHPQMSHNQLQGINKMVGQSVATASTSVGTSGSGIGPGGPGPSTVSGNIPTQWGPAQPVSVGVSGPKDMSKQISGWEEPSPPPQRRSIPNYDDGTSLWGQQTRVPAASGHWKDMTDSIGRSSHLMRGQSQTGGIGIAGVGNSNVPVGANPSNPISSVVGPQARIPSVGGVQHKPDGGAMWVHSGNVGGRNNVAAVTTWGDDTHSVNVGAPSSGSVSSNNWVDDKSNSTLAQNSWSDPAPVGVSWGNKQSKPPSNSASSGWSTAAGVVDGVDLGSEWNTHGGIIGKSQQQQKLAGLNVGMVNVINAEIIKQSKQYRILVENGFKKEDVERALVIANMNIEEAADMLRANSSLSMDGWRRHDESLGSYADHNSSTSSGGFAGRYPVNSGQPSMSFPHNNLMNNMGGTAVTGGNNNTNMTALQVQKYLNQGQHGVAVGPQAVGNSSAVSVGFGQNTSNAAVAGAASVNIAANTNNQPSGQQIRMLGQQIQLAIHSGFISSQILTQPLTQTTLNLLNQLLSNIKHLQAAQQSLTRGGNVNPMAVNVAISKYKQQIQNLQNQINAQQAVYVKQQNMQPTSQQQQPQQQQLPSVHLSNSGNDYLRGHDAINNLQSNFSELNINKPSGYQGASNQQSRLNQWKLPVLDKEINSDSTEFSRAPGATKQNLTANTSNINSLGLQNDSTWSTGRSIGDGWPDPSSDNENKDWSVAQPTSAATAYTDLVQEFEPGKPWKGSQIKSIEDDPSITPGSVARSPLSINSTPKDADIFANTGKNSPTDLPPLSLSSSTWSFNPNQNYPSHSWSDNSQQCTATSELWTSPLNKSSSRGPPPGLTANSNKSANSNASTPTTITGGANGWLQPRSGGVQTTNTNWTGGNTTWGSSWLLLKNLTAQIDGPTLRTLCMQHGPLVSFHPYLNQGIALCKYTTREEANKAQMALNNCVLANTTIFAESPSENEVQSIMQHLPQTPSSTSSSGTSGGNVGGVGTSANNANSGSAACLSGNNSGNGNGSASGAGSGNNGNSSCNNSAAGGGSSSNNTITTVANSNLVGSSGSVSNSSGVTANSSTVSVVSCTASGNSINGAGTANSSGSKSSANNLASGQSSASNLTNSTNSTWRQTSQNQALQSQSRPSGREADFDYISLVYSIVDD

Target ORF information:

RefSeq Version	NM_166784.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gawky (gw), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166784.2

ORF Insert Sequence:

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC 
ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT 
GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG 
TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT 
AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT 
CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA 
ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT 
CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT 
TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT 
GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT 
ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC 
ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA 
CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC 
ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC 
AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC 
AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG 
AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT 
ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA 
AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT 
AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA 
AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC 
ATTGTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gw ( NM_166781.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726597.1 Drosophila melanogaster gawky (gw), transcript variant B, mRNA.

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CloneID	OFa00288
Clone ID Related Accession (Same CDS sequence)	NM_166784.2 , NM_166781.2 , NM_166780.2 , NM_166785.2
Accession Version	NM_166781.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gawky, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166781.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC 
ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT 
GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG 
TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT 
AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT 
CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA 
ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT 
CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT 
TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT 
GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT 
ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC 
ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA 
CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC 
ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC 
AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC 
AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG 
AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT 
ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA 
AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT 
AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA 
AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC 
ATTGTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726597.1
CDS	352..4506
Translation	MREALFSQDGWGCQHVNQDTNWEVPSSPEPANKDAPGPPMWKPSINNGTDLWESNLRNGGQPAAQQVPKPSWGHTPSSNLGGTWGEDDDGADSSSVWTGGAVSNAGSGAAVGVNQAGVNVGPGGVVSSGGPQWGQGVVGVGLGSTGGNGSSNITGSSGVATGSSGNSSNAGNGWGDPREIRPLGVGGSMDIRNVEHRGGNGSGATSSDPRDIRMIDPRDPIRGDPRGISGRLNGTSEMWGHHPQMSHNQLQGINKMVGQSVATASTSVGTSGSGIGPGGPGPSTVSGNIPTQWGPAQPVSVGVSGPKDMSKQISGWEEPSPPPQRRSIPNYDDGTSLWGQQTRVPAASGHWKDMTDSIGRSSHLMRGQSQTGGIGIAGVGNSNVPVGANPSNPISSVVGPQARIPSVGGVQHKPDGGAMWVHSGNVGGRNNVAAVTTWGDDTHSVNVGAPSSGSVSSNNWVDDKSNSTLAQNSWSDPAPVGVSWGNKQSKPPSNSASSGWSTAAGVVDGVDLGSEWNTHGGIIGKSQQQQKLAGLNVGMVNVINAEIIKQSKQYRILVENGFKKEDVERALVIANMNIEEAADMLRANSSLSMDGWRRHDESLGSYADHNSSTSSGGFAGRYPVNSGQPSMSFPHNNLMNNMGGTAVTGGNNNTNMTALQVQKYLNQGQHGVAVGPQAVGNSSAVSVGFGQNTSNAAVAGAASVNIAANTNNQPSGQQIRMLGQQIQLAIHSGFISSQILTQPLTQTTLNLLNQLLSNIKHLQAAQQSLTRGGNVNPMAVNVAISKYKQQIQNLQNQINAQQAVYVKQQNMQPTSQQQQPQQQQLPSVHLSNSGNDYLRGHDAINNLQSNFSELNINKPSGYQGASNQQSRLNQWKLPVLDKEINSDSTEFSRAPGATKQNLTANTSNINSLGLQNDSTWSTGRSIGDGWPDPSSDNENKDWSVAQPTSAATAYTDLVQEFEPGKPWKGSQIKSIEDDPSITPGSVARSPLSINSTPKDADIFANTGKNSPTDLPPLSLSSSTWSFNPNQNYPSHSWSDNSQQCTATSELWTSPLNKSSSRGPPPGLTANSNKSANSNASTPTTITGGANGWLQPRSGGVQTTNTNWTGGNTTWGSSWLLLKNLTAQIDGPTLRTLCMQHGPLVSFHPYLNQGIALCKYTTREEANKAQMALNNCVLANTTIFAESPSENEVQSIMQHLPQTPSSTSSSGTSGGNVGGVGTSANNANSGSAACLSGNNSGNGNGSASGAGSGNNGNSSCNNSAAGGGSSSNNTITTVANSNLVGSSGSVSNSSGVTANSSTVSVVSCTASGNSINGAGTANSSGSKSSANNLASGQSSASNLTNSTNSTWRQTSQNQALQSQSRPSGREADFDYISLVYSIVDD

Target ORF information:

RefSeq Version	NM_166781.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gawky (gw), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166781.2

ORF Insert Sequence:

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC 
ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT 
GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG 
TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT 
AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT 
CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA 
ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT 
CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT 
TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT 
GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT 
ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC 
ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA 
CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC 
ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC 
AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC 
AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG 
AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT 
ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA 
AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT 
AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA 
AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC 
ATTGTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gw ( NM_166780.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726596.1 Drosophila melanogaster gawky (gw), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00288
Clone ID Related Accession (Same CDS sequence)	NM_166784.2 , NM_166781.2 , NM_166780.2 , NM_166785.2
Accession Version	NM_166780.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gawky, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166780.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC 
ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT 
GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG 
TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT 
AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT 
CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA 
ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT 
CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT 
TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT 
GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT 
ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC 
ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA 
CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC 
ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC 
AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC 
AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG 
AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT 
ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA 
AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT 
AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA 
AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC 
ATTGTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726596.1
CDS	596..4750
Translation	MREALFSQDGWGCQHVNQDTNWEVPSSPEPANKDAPGPPMWKPSINNGTDLWESNLRNGGQPAAQQVPKPSWGHTPSSNLGGTWGEDDDGADSSSVWTGGAVSNAGSGAAVGVNQAGVNVGPGGVVSSGGPQWGQGVVGVGLGSTGGNGSSNITGSSGVATGSSGNSSNAGNGWGDPREIRPLGVGGSMDIRNVEHRGGNGSGATSSDPRDIRMIDPRDPIRGDPRGISGRLNGTSEMWGHHPQMSHNQLQGINKMVGQSVATASTSVGTSGSGIGPGGPGPSTVSGNIPTQWGPAQPVSVGVSGPKDMSKQISGWEEPSPPPQRRSIPNYDDGTSLWGQQTRVPAASGHWKDMTDSIGRSSHLMRGQSQTGGIGIAGVGNSNVPVGANPSNPISSVVGPQARIPSVGGVQHKPDGGAMWVHSGNVGGRNNVAAVTTWGDDTHSVNVGAPSSGSVSSNNWVDDKSNSTLAQNSWSDPAPVGVSWGNKQSKPPSNSASSGWSTAAGVVDGVDLGSEWNTHGGIIGKSQQQQKLAGLNVGMVNVINAEIIKQSKQYRILVENGFKKEDVERALVIANMNIEEAADMLRANSSLSMDGWRRHDESLGSYADHNSSTSSGGFAGRYPVNSGQPSMSFPHNNLMNNMGGTAVTGGNNNTNMTALQVQKYLNQGQHGVAVGPQAVGNSSAVSVGFGQNTSNAAVAGAASVNIAANTNNQPSGQQIRMLGQQIQLAIHSGFISSQILTQPLTQTTLNLLNQLLSNIKHLQAAQQSLTRGGNVNPMAVNVAISKYKQQIQNLQNQINAQQAVYVKQQNMQPTSQQQQPQQQQLPSVHLSNSGNDYLRGHDAINNLQSNFSELNINKPSGYQGASNQQSRLNQWKLPVLDKEINSDSTEFSRAPGATKQNLTANTSNINSLGLQNDSTWSTGRSIGDGWPDPSSDNENKDWSVAQPTSAATAYTDLVQEFEPGKPWKGSQIKSIEDDPSITPGSVARSPLSINSTPKDADIFANTGKNSPTDLPPLSLSSSTWSFNPNQNYPSHSWSDNSQQCTATSELWTSPLNKSSSRGPPPGLTANSNKSANSNASTPTTITGGANGWLQPRSGGVQTTNTNWTGGNTTWGSSWLLLKNLTAQIDGPTLRTLCMQHGPLVSFHPYLNQGIALCKYTTREEANKAQMALNNCVLANTTIFAESPSENEVQSIMQHLPQTPSSTSSSGTSGGNVGGVGTSANNANSGSAACLSGNNSGNGNGSASGAGSGNNGNSSCNNSAAGGGSSSNNTITTVANSNLVGSSGSVSNSSGVTANSSTVSVVSCTASGNSINGAGTANSSGSKSSANNLASGQSSASNLTNSTNSTWRQTSQNQALQSQSRPSGREADFDYISLVYSIVDD

Target ORF information:

RefSeq Version	NM_166780.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gawky (gw), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166780.2

ORF Insert Sequence:

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC 
ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT 
GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG 
TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT 
AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT 
CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA 
ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT 
CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT 
TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT 
GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT 
ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC 
ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA 
CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC 
ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC 
AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC 
AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG 
AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT 
ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA 
AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT 
AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA 
AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC 
ATTGTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gw ( NM_166785.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726601.1 Drosophila melanogaster gawky (gw), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa00288
Clone ID Related Accession (Same CDS sequence)	NM_166784.2 , NM_166781.2 , NM_166780.2 , NM_166785.2
Accession Version	NM_166785.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gawky, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166785.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC 
ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT 
GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG 
TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT 
AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT 
CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA 
ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT 
CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT 
TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT 
GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT 
ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC 
ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA 
CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC 
ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC 
AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC 
AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG 
AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT 
ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA 
AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT 
AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA 
AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC 
ATTGTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726601.1
CDS	278..4432
Translation	MREALFSQDGWGCQHVNQDTNWEVPSSPEPANKDAPGPPMWKPSINNGTDLWESNLRNGGQPAAQQVPKPSWGHTPSSNLGGTWGEDDDGADSSSVWTGGAVSNAGSGAAVGVNQAGVNVGPGGVVSSGGPQWGQGVVGVGLGSTGGNGSSNITGSSGVATGSSGNSSNAGNGWGDPREIRPLGVGGSMDIRNVEHRGGNGSGATSSDPRDIRMIDPRDPIRGDPRGISGRLNGTSEMWGHHPQMSHNQLQGINKMVGQSVATASTSVGTSGSGIGPGGPGPSTVSGNIPTQWGPAQPVSVGVSGPKDMSKQISGWEEPSPPPQRRSIPNYDDGTSLWGQQTRVPAASGHWKDMTDSIGRSSHLMRGQSQTGGIGIAGVGNSNVPVGANPSNPISSVVGPQARIPSVGGVQHKPDGGAMWVHSGNVGGRNNVAAVTTWGDDTHSVNVGAPSSGSVSSNNWVDDKSNSTLAQNSWSDPAPVGVSWGNKQSKPPSNSASSGWSTAAGVVDGVDLGSEWNTHGGIIGKSQQQQKLAGLNVGMVNVINAEIIKQSKQYRILVENGFKKEDVERALVIANMNIEEAADMLRANSSLSMDGWRRHDESLGSYADHNSSTSSGGFAGRYPVNSGQPSMSFPHNNLMNNMGGTAVTGGNNNTNMTALQVQKYLNQGQHGVAVGPQAVGNSSAVSVGFGQNTSNAAVAGAASVNIAANTNNQPSGQQIRMLGQQIQLAIHSGFISSQILTQPLTQTTLNLLNQLLSNIKHLQAAQQSLTRGGNVNPMAVNVAISKYKQQIQNLQNQINAQQAVYVKQQNMQPTSQQQQPQQQQLPSVHLSNSGNDYLRGHDAINNLQSNFSELNINKPSGYQGASNQQSRLNQWKLPVLDKEINSDSTEFSRAPGATKQNLTANTSNINSLGLQNDSTWSTGRSIGDGWPDPSSDNENKDWSVAQPTSAATAYTDLVQEFEPGKPWKGSQIKSIEDDPSITPGSVARSPLSINSTPKDADIFANTGKNSPTDLPPLSLSSSTWSFNPNQNYPSHSWSDNSQQCTATSELWTSPLNKSSSRGPPPGLTANSNKSANSNASTPTTITGGANGWLQPRSGGVQTTNTNWTGGNTTWGSSWLLLKNLTAQIDGPTLRTLCMQHGPLVSFHPYLNQGIALCKYTTREEANKAQMALNNCVLANTTIFAESPSENEVQSIMQHLPQTPSSTSSSGTSGGNVGGVGTSANNANSGSAACLSGNNSGNGNGSASGAGSGNNGNSSCNNSAAGGGSSSNNTITTVANSNLVGSSGSVSNSSGVTANSSTVSVVSCTASGNSINGAGTANSSGSKSSANNLASGQSSASNLTNSTNSTWRQTSQNQALQSQSRPSGREADFDYISLVYSIVDD

Target ORF information:

RefSeq Version	NM_166785.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gawky (gw), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166785.2

ORF Insert Sequence:

ATGCGTGAAG CCCTTTTTTC CCAAGATGGC TGGGGCTGTC AGCATGTTAA CCAGGATACT 
AATTGGGAAG TTCCCAGTTC GCCAGAACCA GCCAATAAGG ATGCACCCGG TCCACCAATG 
TGGAAGCCAA GCATTAACAA TGGTACTGAT CTTTGGGAGT CCAATTTGAG AAACGGAGGT 
CAGCCGGCCG CACAGCAAGT TCCAAAGCCG TCGTGGGGTC ATACACCATC CTCTAACTTA 
GGTGGAACAT GGGGTGAGGA CGACGATGGC GCCGATAGTA GTAGTGTGTG GACTGGAGGA 
GCTGTTAGCA ACGCGGGATC CGGAGCTGCA GTGGGAGTAA ACCAAGCCGG AGTTAATGTC 
GGTCCAGGCG GTGTTGTTTC GTCTGGCGGA CCTCAGTGGG GACAAGGTGT CGTTGGCGTC 
GGACTTGGAT CAACTGGAGG TAACGGGTCA AGCAATATAA CTGGATCGTC TGGAGTCGCA 
ACAGGTAGTA GCGGAAACTC CAGCAACGCT GGTAACGGTT GGGGAGACCC TCGTGAAATA 
CGCCCTTTGG GAGTTGGTGG CTCCATGGAT ATTCGAAATG TTGAACATCG CGGCGGTAAC 
GGTTCTGGAG CAACTTCGAG CGATCCACGA GACATTCGCA TGATCGATCC GCGTGACCCT 
ATTCGAGGAG ATCCCCGTGG AATATCTGGT CGTCTTAATG GGACCTCTGA AATGTGGGGT 
CATCATCCAC AAATGTCCCA TAACCAGTTG CAAGGTATCA ACAAAATGGT TGGTCAAAGT 
GTAGCAACTG CCAGCACCAG TGTCGGAACA TCTGGCTCGG GCATCGGTCC TGGAGGTCCC 
GGTCCTAGTA CAGTATCAGG CAATATCCCA ACACAGTGGG GGCCTGCTCA ACCGGTAAGC 
GTTGGTGTAA GTGGTCCCAA AGACATGTCA AAACAGATAA GTGGATGGGA GGAACCATCA 
CCACCGCCTC AGCGTCGCAG TATTCCTAAC TACGATGATG GTACATCGTT GTGGGGTCAG 
CAAACTCGTG TTCCCGCTGC AAGCGGTCAC TGGAAAGACA TGACTGATTC GATAGGTCGT 
AGTAGTCATC TCATGCGTGG CCAAAGCCAA ACGGGAGGTA TAGGAATAGC CGGCGTTGGA 
AATAGCAATG TTCCAGTGGG AGCCAATCCA AGTAATCCTA TAAGCAGTGT AGTTGGACCT 
CAAGCCCGGA TTCCATCTGT GGGCGGCGTA CAACACAAAC CAGACGGCGG CGCTATGTGG 
GTGCATTCCG GCAATGTAGG TGGCAGAAAT AATGTTGCTG CTGTTACTAC TTGGGGAGAT 
GACACTCATA GCGTTAATGT CGGCGCTCCC AGCAGTGGCA GTGTATCCAG CAACAATTGG 
GTTGATGACA AGTCCAACTC AACCTTGGCA CAAAACTCTT GGAGCGACCC GGCCCCTGTT 
GGAGTTAGTT GGGGCAATAA GCAAAGCAAA CCGCCAAGCA ATAGTGCTTC ATCAGGTTGG 
AGCACTGCTG CGGGCGTGGT GGATGGGGTT GATCTAGGAT CTGAGTGGAA CACGCACGGG 
GGGATTATTG GAAAATCTCA GCAACAACAA AAACTAGCGG GACTTAACGT GGGAATGGTG 
AACGTAATTA ACGCGGAGAT CATTAAGCAA AGCAAGCAAT ACAGGATCCT TGTCGAGAAC 
GGCTTTAAAA AGGAAGATGT AGAGCGGGCA TTAGTGATTG CTAATATGAA CATCGAAGAG 
GCAGCCGATA TGCTCCGTGC CAACTCATCC CTATCAATGG ATGGTTGGCG TCGACATGAT 
GAGTCCCTTG GATCTTATGC CGACCACAAT AGTTCAACAA GCAGCGGTGG ATTTGCTGGT 
CGTTACCCGG TCAACAGTGG ACAACCTTCA ATGTCCTTTC CTCATAATAA CCTTATGAAT 
AACATGGGAG GTACCGCTGT TACTGGAGGT AACAACAATA CAAACATGAC AGCTTTACAG 
GTGCAAAAGT ATTTAAATCA AGGGCAACAT GGTGTCGCTG TTGGACCGCA AGCCGTTGGT 
AATTCTTCAG CAGTATCTGT CGGATTTGGT CAGAACACGT CTAACGCAGC AGTGGCAGGA 
GCAGCCTCTG TAAATATAGC AGCAAATACA AACAACCAAC CGTCTGGTCA GCAAATTCGC 
ATGCTAGGCC AGCAAATTCA GTTGGCCATT CATAGTGGTT TCATATCTAG TCAGATATTG 
ACTCAACCGC TAACTCAAAC AACCCTTAAC CTTTTAAACC AACTTCTTAG CAATATTAAG 
CATCTCCAGG CTGCGCAGCA ATCCCTTACC CGCGGGGGAA ATGTCAATCC AATGGCAGTG 
AATGTGGCTA TATCTAAATA CAAGCAGCAA ATCCAGAATT TACAGAACCA GATAAATGCA 
CAACAGGCTG TGTATGTAAA ACAGCAAAAT ATGCAACCAA CTTCACAACA ACAACAGCCC 
CAACAACAGC AACTTCCTTC TGTTCATCTA AGTAACTCAG GCAACGACTA TTTAAGAGGT 
CACGATGCAA TAAATAATTT GCAAAGCAAC TTTTCTGAGC TCAATATTAA TAAGCCAAGT 
GGATATCAAG GAGCGTCCAA TCAACAATCC CGATTAAATC AGTGGAAGCT TCCAGTATTA 
GATAAGGAGA TCAACTCTGA CAGTACGGAA TTTTCTCGTG CCCCAGGTGC AACGAAACAA 
AATTTGACGG CCAACACAAG CAACATAAAC TCTTTGGGTC TTCAAAACGA TAGTACATGG 
TCAACTGGAC GCAGTATTGG TGACGGTTGG CCTGATCCCT CATCTGATAA CGAGAATAAA 
GACTGGTCTG TTGCTCAGCC AACTTCAGCA GCAACTGCTT ACACTGATCT GGTCCAAGAG 
TTTGAGCCAG GCAAGCCATG GAAGGGTTCA CAGATCAAAA GCATAGAAGA TGATCCCAGC 
ATTACACCAG GAAGCGTTGC TAGATCTCCA TTGTCTATTA ATTCGACGCC AAAAGATGCT 
GACATATTTG CCAATACCGG TAAAAATTCA CCGACTGATT TACCGCCACT AAGTTTATCG 
TCGTCTACAT GGAGTTTTAA TCCAAACCAA AATTATCCGA GTCACAGTTG GTCTGACAAT 
AGTCAACAAT GTACCGCCAC TTCGGAGCTT TGGACAAGCC CGCTAAATAA ATCATCGTCT 
CGAGGTCCCC CGCCAGGATT GACTGCCAAT TCAAATAAAT CTGCAAATAG TAATGCGTCA 
ACGCCAACAA CTATTACCGG AGGTGCGAAT GGATGGTTAC AGCCTCGAAG TGGCGGTGTT 
CAAACCACAA ACACTAATTG GACAGGTGGT AACACCACTT GGGGCTCCAG TTGGTTGCTT 
TTGAAAAATC TAACAGCACA GATTGATGGT CCTACTTTGC GTACACTGTG TATGCAGCAT 
GGGCCCCTTG TCAGCTTTCA CCCGTATTTG AACCAAGGAA TTGCCTTATG TAAATATACT 
ACTCGTGAGG AGGCGAACAA GGCGCAAATG GCGTTAAACA ACTGTGTCCT CGCCAACACC 
ACAATATTTG CTGAATCTCC CAGCGAGAAC GAGGTGCAAA GCATTATGCA GCACTTACCA 
CAAACTCCTT CCTCTACAAG CTCTAGTGGA ACTAGTGGTG GCAACGTCGG AGGCGTCGGC 
ACTTCAGCCA ATAATGCAAA CAGTGGTTCT GCAGCTTGTC TGTCCGGAAA CAATAGCGGC 
AACGGAAACG GCAGCGCGAG CGGCGCCGGC AGCGGCAACA ATGGCAACAG TAGCTGCAAC 
AACAGTGCCG CCGGGGGGGG CAGCAGCAGC AACAACACGA TTACCACTGT AGCAAATTCG 
AATCTTGTTG GTTCTAGTGG CTCTGTCTCA AATTCCTCTG GCGTTACTGC TAACTCTAGT 
ACTGTTTCTG TAGTTAGTTG TACAGCGAGT GGGAATTCCA TAAATGGGGC AGGTACTGCA 
AACAGTTCTG GTTCAAAGAG TAGTGCAAAC AATTTAGCTA GCGGCCAGTC TAGCGCTTCT 
AACTTAACTA ATAGCACCAA TTCAACATGG CGACAAACTA GCCAAAACCA AGCTCTTCAA 
AGTCAAAGCA GGCCATCAGG CAGAGAAGCT GACTTTGATT ATATATCTCT CGTTTATTCC 
ATTGTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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gw cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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