Hcf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hcf
Entrez Gene ID	43788
Full Name	Host cell factor
Synonyms	CG1710,Dmel\CG1710,HCF,HCF1,HCF_DROME,Hcf1,anon-WO0172774.48,dHCF,dHcf,dHcf1
General protein information	Preferred Names Host cell factor Names CG1710 gene product from transcript CG1710-RG CG1710-PA CG1710-PB CG1710-PE CG1710-PF CG1710-PG FBgn0039904 Hcf-PA Hcf-PB Hcf-PE Hcf-PF Hcf-PG heat cell factor host cell factor host cell factor 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102B5-102B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_205873.3	NP_995595.1	host cell factor, isoform G
NM_079882.4	NP_524621.2	host cell factor, isoform A
NM_166757.3	NP_726567.2	host cell factor, isoform F
NM_166756.3	NP_726566.1	host cell factor, isoform B
NM_001258491.3	NP_001245420.1	host cell factor, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dHCF possesses Enhancer of TrxG and PcG (ETP) properties.
Title: Drosophila melanogaster dHCF interacts with both PcG and TrxG epigenetic regulators.

down-regulation of dHCF-associated histone acetyltransferase and histone methyltransferase complexes in vivo interferes with the Myc biological activities.
Title: Drosophila Myc interacts with host cell factor (dHCF) to activate transcription and control growth.

Data demonstrate that the Drosophila homologs of mixed-lineage leukemia protein and host cell factor 1, called Trithorax and dHCF, are both cleaved by Drosophila taspase 1.
Title: Species selectivity of mixed-lineage leukemia/trithorax and HCF proteolytic maturation pathways.

The following Hcf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00158	NM_001258491.3 Latest version!	Drosophila melanogaster host cell factor, transcript variant E (Hcf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa00262	NM_205873.3 Latest version!	Drosophila melanogaster host cell factor, transcript variant G (Hcf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa00262	NM_079882.4 Latest version!	Drosophila melanogaster host cell factor, transcript variant A (Hcf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa00262	NM_166756.3 Latest version!	Drosophila melanogaster host cell factor, transcript variant B (Hcf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa00263	NM_166757.3 Latest version!	Drosophila melanogaster host cell factor, transcript variant F (Hcf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hcf ( NM_001258491.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245420.1 Drosophila melanogaster host cell factor, transcript variant E (Hcf), mRNA.

Price & Availability↑

CloneID	OFa00158
Clone ID Related Accession (Same CDS sequence)	NM_001258491.3
Accession Version	NM_001258491.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	host cell factor, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jul 15, 2014 this sequence version replaced NM_001258491.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGGCT CAGACTTTGT GGATCCAGCC TTTTCTTCAG GAGAACGAAT ATCGGCATCT 
GATCTCAATT CGGAGCATAT TATTCAGGCC GAGAATCACA GTTTTGCTAA TCGAATTTCT 
ATGGATATGG ATGTCCCTGA TGGGCACCAG CTGGACAGCA ACTTAACCGG ATTTCGATGG 
AAGCGAGTTC TTAACCCAAC CGGACCGCAG CCCCGCCCGA GACATGGACA CCGTGCGATA 
AACATCAAAG AGCTAATGGT CGTCTTCGGC GGCGGAAACG AAGGAATTGT TGACGAGTTG 
CACGTTTATA ATACTGTTAC TAATCAGTGG TACGTCCCAG TGCTAAAAGG TGATGTACCG 
AACGGATGCG CTGCTTATGG ATTTGTTGTG GAGGGTACTC GCATGTTTGT TTTTGGGGGA 
ATGATCGAAT ACGGAAAATA TTCAAACGAG CTCTATGAGC TACAGGCAAC TAAATGGGAA 
TGGAGAAAAA TGTATCCAGA GTCACCAGAC AGTGGGTTGT CACCGTGTCC TCGCTTGGGT 
CACAGCTTTA CAATGGTTGG TGAAAAAATA TTTCTTTTCG GCGGACTAGC AAATGAATCG 
GACGACCCAA AAAATAATAT TCCAAAATAC TTAAACGACT TGTACATACT GGATACTCGA 
GGAGTTCACA GTCATAACGG AAAGTGGATA GTACCTAAAA CATATGGGGA TAGCCCGCCA 
CCACGAGAGT CGCACACAGG CATATCGTTT GCTACTAAAA GCAACGGAAA TTTAAATCTT 
TTGATTTATG GTGGAATGAG CGGCTGCCGC TTGGGAGATT TATGGTTGCT AGAAACAGAC 
TCCATGACTT GGTCGAAGCC GAAAACTTCA GGGGAGGCGC CGCTACCACG CTCGTTGCAC 
AGTTCTACAA TGATTGGCAA CAAAATGTAT GTCTTTGGTG GCTGGGTTCC CTTAGTGATC 
AATGATTCTA AATCGACAAC AGAGCGTGAA TGGAAATGTA CAAACACTCT TGCTGTCCTT 
GACCTTGAAA CAATGACATG GGAAAATGTC ACATTGGACA CTGTAGAGGA AAATGTTCCA 
CGTGCCCGTG CTGGCCACTG TGCAGTTGGT ATTCAAAGTC GTCTTTATGT GTGGTCAGGT 
CGTGATGGTT ACCGCAAAGC ATGGAACAAC CAGGTTAGGG TTTGCTGCAA AGACCTGTGG 
TACTTAGAGG TCTCCAAGCC CCTGTATGCA GTAAAAGTTG CGCTAGTTCG CGCTTCTACT 
CACGCCTTGG AGCTCTCGTG GACTGCGACC ACTTTTGCAG CAGCCTACGT TTTGCAAATC 
CAAAAAATTG AGCAGCCATT AAATACAAGC TCGAAACTGT TAAGCAACAA CATTGTTCAA 
CAAGGAACCC CGACATCTGC TGAAACTTCT GGAATTAATA TTTCAGCAAA TAGATCTGGG 
AGTGCTTTGG GTCTTGGTGT CGAAGCCACA TCTACAGTCT TAAAACTAGA AAAGGAATCT 
TTGCAACTGA GTGGATGTCA ACCGGAAACA AATGTTCAAC CATCTGTAAA TGACTTGTTG 
CAATCAATGT CGCAACCTTC TTCCCCAGCG TCCCGTGCTG ATAAAGACCC ACTTTCGTCA 
GGGGGTGGAA CTACCTTTAA CTTATCAACA TCGGTCGCTT CAGTGCATCC TCAAATATCT 
GTAATCAGTA GTACAGCCGC AGTAACGGGC AACGACACAG CGTCCCCAAG TGGCGCCATT 
AATAGTATAT TGCAAAAGTT TCGACCAGTT GTTACCGCTG TAAGAACGTC CACAACTACT 
GCGGTATCTA TTGCTACCAG CACGTCTGAT CCCTTGTCTG TGCGAGTACC AAGTACGATG 
TCGGCAAACG TTGTTCTCAG CTCTTCAAGT AGTACATTGC GTATTGTCCC AAGCGTAACT 
GCATCGCATT CACTACGTAT AGCTTCTTCT CAAGCAAGTG GCAACAACTG CCGGAGTAGC 
TCTGCAATAA ATATATTAAA AACAGCTCTT CCAAACGTCG CCGTGCAATC TCAGCCTACA 
TCATCGACCA CCACCTCCAT TGGTGGAAAA CAGTACTTCA TTCAAAAGCC ACTTACACTT 
GCTCCAAATG TTCAGCTCCA ATTTGTTAAG ACCAGTGGTG GGATGACTGT TCAAACTCTT 
CCAAAAGTAA ACTTTACGGC TTCAAAAGGA ACGCCCCCAC ATGGCATATC AATTGCAAAC 
CCACATTTGG CGTCTGGTAT TACTCAAATT CAGGGATCGA CAGTGCCTGG AAGTCAGATT 
CAAAAGCCCA TTGTGTCCGG AAACGTTTTA AAGCTTGTAT CGCCGCATAC GATGGCCGGT 
GGAAAATTGA TTATGAAGAA TTCAAACATC TTACAGATGG GCAAGGGTAC GCCATTGGGA 
AATCAGCAGA TTATTATTGT AACCACTGGT GGCAATGTGC GATCTGTTCC AACTAGCACA 
GTTATGACAA GTGCTGGTGG CTCTGCATCA GGGACAAATA TTGTAAGTAT AGTTAACTCA 
ACGTCGACCA CGCCTAGCCC GCTGCAGGCA TTAAGTGGAC AAAAAACTTT AATATCCAAC 
CAAAGTGGAG TTAAGATGCT GCGAAATATA TCATCAGTAC AAGCATCATC TTCAATGGCA 
TTTGGGCAAA AACAAAGTGG CACTCCCATT CATCAAAAAA CGGCATTGTA TATAGGAGGC 
AAAGCTGTTA CCGTTATGAG CACCAATACA AGTATGGCTG CATCCGGAAA TAAAGTTATG 
GTACTACCAG GAACAAGCTC AAATAATTCT CCTGCTACTA CAACGGCGCT GAGTGCCAGA 
AAAAGTTTTG TTTTCAATGC CGGAGGTAGC CCTCGGACCG TCACTTTAGC AACTAAAAGC 
ATTAATGCAA AATCTATACC ACAATCGCAA CCAGTGACGG AAACTAATAA CCATTCAGTC 
GCTACTATTA AAGACACAGA TCCAATGGAT GATATTATAG AGCAGTTGGA TGGAGCCGGA 
GATCTGCTAA AGCTTTCTGA AAGTGAAGGC CAGCATGGAT CGGAGGAGAA TGAAAATAAT 
GGTGAAAACG CAACGTCTTC ATCTGCCTCT GCCCTGTTTA CCGGAGGTGA TACGGCCGGG 
CCATCGAGAG CCCAAAACCC TATAGTAATG GAACATCCGG TTGATATTAT AGAAGATGTC 
TCTGGCGTGA GCAGCACAAC TGACGTTAAC GAAACTGCAA TCGTAAGTGG TGATACAATA 
GAATCGCTTA AGATGTCGGA GAAGGAAAAC GATGATGTGA AATCTATGGG TGAAAAATCA 
ATATTATCCG ATGATTGTCA TCAGCCGACG ACTTCCGAGA CGGAAGCGGC CACCATCCTA 
ACCACAATTA AGTCTGCAGA GGCTTTAGTG TTGGAAACTG CAGAAATAAG GAAAGATCAT 
ACCGGATGTA CTATAGGTAG TTTGAAAGAA AATCAAGATG AAAATAAAAA GTTTAAACAA 
CGGCAAGAAT CTTCACCGTC TCAAAACATT CACCAATTCC AAAATGTGGA TGGTTCACAA 
TTAGAGGCTC TTGCTTCTGC TGCGTTACTG CAAGCTGCAA CATCAGACAC CACAGCATTG 
GCGTTGAAAG AATTGATAGA GCGACCGGAA AGTGAAACAA ATACCAGAAG TAGCAACATT 
GCTGAGATTC AACAAAATAA TGTTCAATCC ACCTTAGCGG TCGTGGTTCC AAACACCTCC 
CAAAACGAGA ACCAAAAATG GCACACAGTA GGTGTGTTTA AGGATCTTTC TCACACAGTG 
ACTAGCTACA TTGATTCGAA TTGTATTAGT GATTCTTTTT TTGACGGTAT AGATGTGGAT 
AATCTTCCAG ATTTCAGCAA GTTTCCACGC ACCAATTTGG AGCCTGGAAC GGCTTACCGT 
TTTCGACTGA GTGCTATCAA TTCTTGCGGA CGAGGAGAAT GGGGAGAGAT ATCAAGTTTT 
AAAACCTGTT TACCAGGCTT TCCGGGTGCC CCCTCAGCTA TTAAAATTTC CAAGGATGTC 
AAGGAGGGCG CTCACTTAAC ATGGGAACCA CCACCAGCTC AAAAAACTAA GGAGATAATT 
GAATATTCAG TATACCTTGC AGTTAAGCCT ACTGCCAAGG ATAAGGCGTT GTCCACTCCA 
CAATTAGCTT TTGTGCGGGT TTATGTAGGT GCGGCAAATC AATGCACAGT GCCGAATGCT 
TCACTATCTA ATGCACATGT GGATTGCTCA AATAAACCAG CAATTATATT CCGAATCGCT 
GCTCGCAATC AAAAGGGCTA TGGACCTGCC ACCCAAGTTA GATGGCTGCA GGATCCCGCG 
GCAGCGAAAC AGCACACACC CACAGTTACG CCAAATCTAA AACGTGGACC AGAAAAATCA 
ACTATTGGAA GCAGCAACAT AGCAAACACC TTCTGTTCAC CACACAAGCG TGGACGCAAC 
GGATTGCATG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245420.1
CDS	526..4980
Translation	MEGSDFVDPAFSSGERISASDLNSEHIIQAENHSFANRISMDMDVPDGHQLDSNLTGFRWKRVLNPTGPQPRPRHGHRAINIKELMVVFGGGNEGIVDELHVYNTVTNQWYVPVLKGDVPNGCAAYGFVVEGTRMFVFGGMIEYGKYSNELYELQATKWEWRKMYPESPDSGLSPCPRLGHSFTMVGEKIFLFGGLANESDDPKNNIPKYLNDLYILDTRGVHSHNGKWIVPKTYGDSPPPRESHTGISFATKSNGNLNLLIYGGMSGCRLGDLWLLETDSMTWSKPKTSGEAPLPRSLHSSTMIGNKMYVFGGWVPLVINDSKSTTEREWKCTNTLAVLDLETMTWENVTLDTVEENVPRARAGHCAVGIQSRLYVWSGRDGYRKAWNNQVRVCCKDLWYLEVSKPLYAVKVALVRASTHALELSWTATTFAAAYVLQIQKIEQPLNTSSKLLSNNIVQQGTPTSAETSGINISANRSGSALGLGVEATSTVLKLEKESLQLSGCQPETNVQPSVNDLLQSMSQPSSPASRADKDPLSSGGGTTFNLSTSVASVHPQISVISSTAAVTGNDTASPSGAINSILQKFRPVVTAVRTSTTTAVSIATSTSDPLSVRVPSTMSANVVLSSSSSTLRIVPSVTASHSLRIASSQASGNNCRSSSAINILKTALPNVAVQSQPTSSTTTSIGGKQYFIQKPLTLAPNVQLQFVKTSGGMTVQTLPKVNFTASKGTPPHGISIANPHLASGITQIQGSTVPGSQIQKPIVSGNVLKLVSPHTMAGGKLIMKNSNILQMGKGTPLGNQQIIIVTTGGNVRSVPTSTVMTSAGGSASGTNIVSIVNSTSTTPSPLQALSGQKTLISNQSGVKMLRNISSVQASSSMAFGQKQSGTPIHQKTALYIGGKAVTVMSTNTSMAASGNKVMVLPGTSSNNSPATTTALSARKSFVFNAGGSPRTVTLATKSINAKSIPQSQPVTETNNHSVATIKDTDPMDDIIEQLDGAGDLLKLSESEGQHGSEENENNGENATSSSASALFTGGDTAGPSRAQNPIVMEHPVDIIEDVSGVSSTTDVNETAIVSGDTIESLKMSEKENDDVKSMGEKSILSDDCHQPTTSETEAATILTTIKSAEALVLETAEIRKDHTGCTIGSLKENQDENKKFKQRQESSPSQNIHQFQNVDGSQLEALASAALLQAATSDTTALALKELIERPESETNTRSSNIAEIQQNNVQSTLAVVVPNTSQNENQKWHTVGVFKDLSHTVTSYIDSNCISDSFFDGIDVDNLPDFSKFPRTNLEPGTAYRFRLSAINSCGRGEWGEISSFKTCLPGFPGAPSAIKISKDVKEGAHLTWEPPPAQKTKEIIEYSVYLAVKPTAKDKALSTPQLAFVRVYVGAANQCTVPNASLSNAHVDCSNKPAIIFRIAARNQKGYGPATQVRWLQDPAAAKQHTPTVTPNLKRGPEKSTIGSSNIANTFCSPHKRGRNGLHD

Target ORF information:

RefSeq Version	NM_001258491.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster host cell factor, transcript variant E (Hcf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258491.3

ORF Insert Sequence:

ATGGAAGGCT CAGACTTTGT GGATCCAGCC TTTTCTTCAG GAGAACGAAT ATCGGCATCT 
GATCTCAATT CGGAGCATAT TATTCAGGCC GAGAATCACA GTTTTGCTAA TCGAATTTCT 
ATGGATATGG ATGTCCCTGA TGGGCACCAG CTGGACAGCA ACTTAACCGG ATTTCGATGG 
AAGCGAGTTC TTAACCCAAC CGGACCGCAG CCCCGCCCGA GACATGGACA CCGTGCGATA 
AACATCAAAG AGCTAATGGT CGTCTTCGGC GGCGGAAACG AAGGAATTGT TGACGAGTTG 
CACGTTTATA ATACTGTTAC TAATCAGTGG TACGTCCCAG TGCTAAAAGG TGATGTACCG 
AACGGATGCG CTGCTTATGG ATTTGTTGTG GAGGGTACTC GCATGTTTGT TTTTGGGGGA 
ATGATCGAAT ACGGAAAATA TTCAAACGAG CTCTATGAGC TACAGGCAAC TAAATGGGAA 
TGGAGAAAAA TGTATCCAGA GTCACCAGAC AGTGGGTTGT CACCGTGTCC TCGCTTGGGT 
CACAGCTTTA CAATGGTTGG TGAAAAAATA TTTCTTTTCG GCGGACTAGC AAATGAATCG 
GACGACCCAA AAAATAATAT TCCAAAATAC TTAAACGACT TGTACATACT GGATACTCGA 
GGAGTTCACA GTCATAACGG AAAGTGGATA GTACCTAAAA CATATGGGGA TAGCCCGCCA 
CCACGAGAGT CGCACACAGG CATATCGTTT GCTACTAAAA GCAACGGAAA TTTAAATCTT 
TTGATTTATG GTGGAATGAG CGGCTGCCGC TTGGGAGATT TATGGTTGCT AGAAACAGAC 
TCCATGACTT GGTCGAAGCC GAAAACTTCA GGGGAGGCGC CGCTACCACG CTCGTTGCAC 
AGTTCTACAA TGATTGGCAA CAAAATGTAT GTCTTTGGTG GCTGGGTTCC CTTAGTGATC 
AATGATTCTA AATCGACAAC AGAGCGTGAA TGGAAATGTA CAAACACTCT TGCTGTCCTT 
GACCTTGAAA CAATGACATG GGAAAATGTC ACATTGGACA CTGTAGAGGA AAATGTTCCA 
CGTGCCCGTG CTGGCCACTG TGCAGTTGGT ATTCAAAGTC GTCTTTATGT GTGGTCAGGT 
CGTGATGGTT ACCGCAAAGC ATGGAACAAC CAGGTTAGGG TTTGCTGCAA AGACCTGTGG 
TACTTAGAGG TCTCCAAGCC CCTGTATGCA GTAAAAGTTG CGCTAGTTCG CGCTTCTACT 
CACGCCTTGG AGCTCTCGTG GACTGCGACC ACTTTTGCAG CAGCCTACGT TTTGCAAATC 
CAAAAAATTG AGCAGCCATT AAATACAAGC TCGAAACTGT TAAGCAACAA CATTGTTCAA 
CAAGGAACCC CGACATCTGC TGAAACTTCT GGAATTAATA TTTCAGCAAA TAGATCTGGG 
AGTGCTTTGG GTCTTGGTGT CGAAGCCACA TCTACAGTCT TAAAACTAGA AAAGGAATCT 
TTGCAACTGA GTGGATGTCA ACCGGAAACA AATGTTCAAC CATCTGTAAA TGACTTGTTG 
CAATCAATGT CGCAACCTTC TTCCCCAGCG TCCCGTGCTG ATAAAGACCC ACTTTCGTCA 
GGGGGTGGAA CTACCTTTAA CTTATCAACA TCGGTCGCTT CAGTGCATCC TCAAATATCT 
GTAATCAGTA GTACAGCCGC AGTAACGGGC AACGACACAG CGTCCCCAAG TGGCGCCATT 
AATAGTATAT TGCAAAAGTT TCGACCAGTT GTTACCGCTG TAAGAACGTC CACAACTACT 
GCGGTATCTA TTGCTACCAG CACGTCTGAT CCCTTGTCTG TGCGAGTACC AAGTACGATG 
TCGGCAAACG TTGTTCTCAG CTCTTCAAGT AGTACATTGC GTATTGTCCC AAGCGTAACT 
GCATCGCATT CACTACGTAT AGCTTCTTCT CAAGCAAGTG GCAACAACTG CCGGAGTAGC 
TCTGCAATAA ATATATTAAA AACAGCTCTT CCAAACGTCG CCGTGCAATC TCAGCCTACA 
TCATCGACCA CCACCTCCAT TGGTGGAAAA CAGTACTTCA TTCAAAAGCC ACTTACACTT 
GCTCCAAATG TTCAGCTCCA ATTTGTTAAG ACCAGTGGTG GGATGACTGT TCAAACTCTT 
CCAAAAGTAA ACTTTACGGC TTCAAAAGGA ACGCCCCCAC ATGGCATATC AATTGCAAAC 
CCACATTTGG CGTCTGGTAT TACTCAAATT CAGGGATCGA CAGTGCCTGG AAGTCAGATT 
CAAAAGCCCA TTGTGTCCGG AAACGTTTTA AAGCTTGTAT CGCCGCATAC GATGGCCGGT 
GGAAAATTGA TTATGAAGAA TTCAAACATC TTACAGATGG GCAAGGGTAC GCCATTGGGA 
AATCAGCAGA TTATTATTGT AACCACTGGT GGCAATGTGC GATCTGTTCC AACTAGCACA 
GTTATGACAA GTGCTGGTGG CTCTGCATCA GGGACAAATA TTGTAAGTAT AGTTAACTCA 
ACGTCGACCA CGCCTAGCCC GCTGCAGGCA TTAAGTGGAC AAAAAACTTT AATATCCAAC 
CAAAGTGGAG TTAAGATGCT GCGAAATATA TCATCAGTAC AAGCATCATC TTCAATGGCA 
TTTGGGCAAA AACAAAGTGG CACTCCCATT CATCAAAAAA CGGCATTGTA TATAGGAGGC 
AAAGCTGTTA CCGTTATGAG CACCAATACA AGTATGGCTG CATCCGGAAA TAAAGTTATG 
GTACTACCAG GAACAAGCTC AAATAATTCT CCTGCTACTA CAACGGCGCT GAGTGCCAGA 
AAAAGTTTTG TTTTCAATGC CGGAGGTAGC CCTCGGACCG TCACTTTAGC AACTAAAAGC 
ATTAATGCAA AATCTATACC ACAATCGCAA CCAGTGACGG AAACTAATAA CCATTCAGTC 
GCTACTATTA AAGACACAGA TCCAATGGAT GATATTATAG AGCAGTTGGA TGGAGCCGGA 
GATCTGCTAA AGCTTTCTGA AAGTGAAGGC CAGCATGGAT CGGAGGAGAA TGAAAATAAT 
GGTGAAAACG CAACGTCTTC ATCTGCCTCT GCCCTGTTTA CCGGAGGTGA TACGGCCGGG 
CCATCGAGAG CCCAAAACCC TATAGTAATG GAACATCCGG TTGATATTAT AGAAGATGTC 
TCTGGCGTGA GCAGCACAAC TGACGTTAAC GAAACTGCAA TCGTAAGTGG TGATACAATA 
GAATCGCTTA AGATGTCGGA GAAGGAAAAC GATGATGTGA AATCTATGGG TGAAAAATCA 
ATATTATCCG ATGATTGTCA TCAGCCGACG ACTTCCGAGA CGGAAGCGGC CACCATCCTA 
ACCACAATTA AGTCTGCAGA GGCTTTAGTG TTGGAAACTG CAGAAATAAG GAAAGATCAT 
ACCGGATGTA CTATAGGTAG TTTGAAAGAA AATCAAGATG AAAATAAAAA GTTTAAACAA 
CGGCAAGAAT CTTCACCGTC TCAAAACATT CACCAATTCC AAAATGTGGA TGGTTCACAA 
TTAGAGGCTC TTGCTTCTGC TGCGTTACTG CAAGCTGCAA CATCAGACAC CACAGCATTG 
GCGTTGAAAG AATTGATAGA GCGACCGGAA AGTGAAACAA ATACCAGAAG TAGCAACATT 
GCTGAGATTC AACAAAATAA TGTTCAATCC ACCTTAGCGG TCGTGGTTCC AAACACCTCC 
CAAAACGAGA ACCAAAAATG GCACACAGTA GGTGTGTTTA AGGATCTTTC TCACACAGTG 
ACTAGCTACA TTGATTCGAA TTGTATTAGT GATTCTTTTT TTGACGGTAT AGATGTGGAT 
AATCTTCCAG ATTTCAGCAA GTTTCCACGC ACCAATTTGG AGCCTGGAAC GGCTTACCGT 
TTTCGACTGA GTGCTATCAA TTCTTGCGGA CGAGGAGAAT GGGGAGAGAT ATCAAGTTTT 
AAAACCTGTT TACCAGGCTT TCCGGGTGCC CCCTCAGCTA TTAAAATTTC CAAGGATGTC 
AAGGAGGGCG CTCACTTAAC ATGGGAACCA CCACCAGCTC AAAAAACTAA GGAGATAATT 
GAATATTCAG TATACCTTGC AGTTAAGCCT ACTGCCAAGG ATAAGGCGTT GTCCACTCCA 
CAATTAGCTT TTGTGCGGGT TTATGTAGGT GCGGCAAATC AATGCACAGT GCCGAATGCT 
TCACTATCTA ATGCACATGT GGATTGCTCA AATAAACCAG CAATTATATT CCGAATCGCT 
GCTCGCAATC AAAAGGGCTA TGGACCTGCC ACCCAAGTTA GATGGCTGCA GGATCCCGCG 
GCAGCGAAAC AGCACACACC CACAGTTACG CCAAATCTAA AACGTGGACC AGAAAAATCA 
ACTATTGGAA GCAGCAACAT AGCAAACACC TTCTGTTCAC CACACAAGCG TGGACGCAAC 
GGATTGCATG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcf ( NM_205873.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995595.1 Drosophila melanogaster host cell factor, transcript variant G (Hcf), mRNA.

Price & Availability↑

CloneID	OFa00262
Clone ID Related Accession (Same CDS sequence)	NM_205873.3 , NM_079882.4 , NM_166756.3
Accession Version	NM_205873.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	host cell factor, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jul 15, 2014 this sequence version replaced NM_205873.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGGCT CAGACTTTGT GGATCCAGCC TTTTCTTCAG GAGAACGAAT ATCGGCATCT 
GATCTCAATT CGGAGCATAT TATTCAGGCC GAGAATCACA GTTTTGCTAA TCGAATTTCT 
ATGGATATGG ATGTCCCTGA TGGGCACCAG CTGGACAGCA ACTTAACCGG ATTTCGATGG 
AAGCGAGTTC TTAACCCAAC CGGACCGCAG CCCCGCCCGA GACATGGACA CCGTGCGATA 
AACATCAAAG AGCTAATGGT CGTCTTCGGC GGCGGAAACG AAGGAATTGT TGACGAGTTG 
CACGTTTATA ATACTGTTAC TAATCAGTGG TACGTCCCAG TGCTAAAAGG TGATGTACCG 
AACGGATGCG CTGCTTATGG ATTTGTTGTG GAGGGTACTC GCATGTTTGT TTTTGGGGGA 
ATGATCGAAT ACGGAAAATA TTCAAACGAG CTCTATGAGC TACAGGCAAC TAAATGGGAA 
TGGAGAAAAA TGTATCCAGA GTCACCAGAC AGTGGGTTGT CACCGTGTCC TCGCTTGGGT 
CACAGCTTTA CAATGGTTGG TGAAAAAATA TTTCTTTTCG GCGGACTAGC AAATGAATCG 
GACGACCCAA AAAATAATAT TCCAAAATAC TTAAACGACT TGTACATACT GGATACTCGA 
GGAGTTCACA GTCATAACGG AAAGTGGATA GTACCTAAAA CATATGGGGA TAGCCCGCCA 
CCACGAGAGT CGCACACAGG CATATCGTTT GCTACTAAAA GCAACGGAAA TTTAAATCTT 
TTGATTTATG GTGGAATGAG CGGCTGCCGC TTGGGAGATT TATGGTTGCT AGAAACAGAC 
TCCATGACTT GGTCGAAGCC GAAAACTTCA GGGGAGGCGC CGCTACCACG CTCGTTGCAC 
AGTTCTACAA TGATTGGCAA CAAAATGTAT GTCTTTGGTG GCTGGGTTCC CTTAGTGATC 
AATGATTCTA AATCGACAAC AGAGCGTGAA TGGAAATGTA CAAACACTCT TGCTGTCCTT 
GACCTTGAAA CAATGACATG GGAAAATGTC ACATTGGACA CTGTAGAGGA AAATGTTCCA 
CGTGCCCGTG CTGGCCACTG TGCAGTTGGT ATTCAAAGTC GTCTTTATGT GTGGTCAGGT 
CGTGATGGTT ACCGCAAAGC ATGGAACAAC CAGGTTTGCT GCAAAGACCT GTGGTACTTA 
GAGGTCTCCA AGCCCCTGTA TGCAGTAAAA GTTGCGCTAG TTCGCGCTTC TACTCACGCC 
TTGGAGCTCT CGTGGACTGC GACCACTTTT GCAGCAGCCT ACGTTTTGCA AATCCAAAAA 
ATTGAGCAGC CATTAAATAC AAGCTCGAAA CTGTTAAGCA ACAACATTGT TCAACAAGGA 
ACCCCGACAT CTGCTGAAAC TTCTGGAATT AATATTTCAG CAAATAGATC TGGGAGTGCT 
TTGGGTCTTG GTGTCGAAGC CACATCTACA GTCTTAAAAC TAGAAAAGGA ATCTTTGCAA 
CTGAGTGGAT GTCAACCGGA AACAAATGTT CAACCATCTG TAAATGACTT GTTGCAATCA 
ATGTCGCAAC CTTCTTCCCC AGCGTCCCGT GCTGATAAAG ACCCACTTTC GTCAGGGGGT 
GGAACTACCT TTAACTTATC AACATCGGTC GCTTCAGTGC ATCCTCAAAT ATCTGTAATC 
AGTAGTACAG CCGCAGTAAC GGGCAACGAC ACAGCGTCCC CAAGTGGCGC CATTAATAGT 
ATATTGCAAA AGTTTCGACC AGTTGTTACC GCTGTAAGAA CGTCCACAAC TACTGCGGTA 
TCTATTGCTA CCAGCACGTC TGATCCCTTG TCTGTGCGAG TACCAAGTAC GATGTCGGCA 
AACGTTGTTC TCAGCTCTTC AAGTAGTACA TTGCGTATTG TCCCAAGCGT AACTGCATCG 
CATTCACTAC GTATAGCTTC TTCTCAAGCA AGTGGCAACA ACTGCCGGAG TAGCTCTGCA 
ATAAATATAT TAAAAACAGC TCTTCCAAAC GTCGCCGTGC AATCTCAGCC TACATCATCG 
ACCACCACCT CCATTGGTGG AAAACAGTAC TTCATTCAAA AGCCACTTAC ACTTGCTCCA 
AATGTTCAGC TCCAATTTGT TAAGACCAGT GGTGGGATGA CTGTTCAAAC TCTTCCAAAA 
GTAAACTTTA CGGCTTCAAA AGGAACGCCC CCACATGGCA TATCAATTGC AAACCCACAT 
TTGGCGTCTG GTATTACTCA AATTCAGGGA TCGACAGTGC CTGGAAGTCA GATTCAAAAG 
CCCATTGTGT CCGGAAACGT TTTAAAGCTT GTATCGCCGC ATACGATGGC CGGTGGAAAA 
TTGATTATGA AGAATTCAAA CATCTTACAG ATGGGCAAGG TAACTCCGAA CGTAATGGGT 
GGAAAACCAG CTTTTGTCAT AACTAATAAA CAGGGTACGC CATTGGGAAA TCAGCAGATT 
ATTATTGTAA CCACTGGTGG CAATGTGCGA TCTGTTCCAA CTAGCACAGT TATGACAAGT 
GCTGGTGGCT CTGCATCAGG GACAAATATT GTAAGTATAG TTAACTCAAC GTCGACCACG 
CCTAGCCCGC TGCAGGCATT AAGTGGACAA AAAACTTTAA TATCCAACCA AAGTGGAGTT 
AAGATGCTGC GAAATATATC ATCAGTACAA GCATCATCTT CAATGGCATT TGGGCAAAAA 
CAAAGTGGCA CTCCCATTCA TCAAAAAACG GCATTGTATA TAGGAGGCAA AGCTGTTACC 
GTTATGAGCA CCAATACAAG TATGGCTGCA TCCGGAAATA AAGTTATGGT ACTACCAGGA 
ACAAGCTCAA ATAATTCTCC TGCTACTACA ACGGCGCTGA GTGCCAGAAA AAGTTTTGTT 
TTCAATGCCG GAGGTAGCCC TCGGACCGTC ACTTTAGCAA CTAAAAGCAT TAATGCAAAA 
TCTATACCAC AATCGCAACC AGTGACGGAA ACTAATAACC ATTCAGTCGC TACTATTAAA 
GACACAGATC CAATGGATGA TATTATAGAG CAGTTGGATG GAGCCGGAGA TCTGCTAAAG 
CTTTCTGAAA GTGAAGGCCA GCATGGATCG GAGGAGAATG AAAATAATGG TGAAAACGCA 
ACGTCTTCAT CTGCCTCTGC CCTGTTTACC GGAGGTGATA CGGCCGGGCC ATCGAGAGCC 
CAAAACCCTA TAGTAATGGA ACATCCGGTT GATATTATAG AAGATGTCTC TGGCGTGAGC 
AGCACAACTG ACGTTAACGA AACTGCAATC GTAAGTGGTG ATACAATAGA ATCGCTTAAG 
ATGTCGGAGA AGGAAAACGA TGATGTGAAA TCTATGGGTG AAAAATCAAT ATTATCCGAT 
GATTGTCATC AGCCGACGAC TTCCGAGACG GAAGCGGCCA CCATCCTAAC CACAATTAAG 
TCTGCAGAGG CTTTAGTGTT GGAAACTGCA GAAATAAGGA AAGATCATAC CGGATGTACT 
ATAGGTAGTT TGAAAGAAAA TCAAGATGAA AATAAAAAGT TTAAACAACG GCAAGAATCT 
TCACCGTCTC AAAACATTCA CCAATTCCAA AATGTGGATG GTTCACAATT AGAGGCTCTT 
GCTTCTGCTG CGTTACTGCA AGCTGCAACA TCAGACACCA CAGCATTGGC GTTGAAAGAA 
TTGATAGAGC GACCGGAAAG TGAAACAAAT ACCAGAAGTA GCAACATTGC TGAGATTCAA 
CAAAATAATG TTCAATCCAC CTTAGCGGTC GTGGTTCCAA ACACCTCCCA AAACGAGAAC 
CAAAAATGGC ACACAGTAGG TGTGTTTAAG GATCTTTCTC ACACAGTGAC TAGCTACATT 
GATTCGAATT GTATTAGTGA TTCTTTTTTT GACGGTATAG ATGTGGATAA TCTTCCAGAT 
TTCAGCAAGT TTCCACGCAC CAATTTGGAG CCTGGAACGG CTTACCGTTT TCGACTGAGT 
GCTATCAATT CTTGCGGACG AGGAGAATGG GGAGAGATAT CAAGTTTTAA AACCTGTTTA 
CCAGGCTTTC CGGGTGCCCC CTCAGCTATT AAAATTTCCA AGGATGTCAA GGAGGGCGCT 
CACTTAACAT GGGAACCACC ACCAGCTCAA AAAACTAAGG AGATAATTGA ATATTCAGTA 
TACCTTGCAG TTAAGCCTAC TGCCAAGGAT AAGGCGTTGT CCACTCCACA ATTAGCTTTT 
GTGCGGGTTT ATGTAGGTGC GGCAAATCAA TGCACAGTGC CGAATGCTTC ACTATCTAAT 
GCACATGTGG ATTGCTCAAA TAAACCAGCA ATTATATTCC GAATCGCTGC TCGCAATCAA 
AAGGGCTATG GACCTGCCAC CCAAGTTAGA TGGCTGCAGG ATCCCGCGGC AGCGAAACAG 
CACACACCCA CAGTTACGCC AAATCTAAAA CGTGGACCAG AAAAATCAAC TATTGGAAGC 
AGCAACATAG CAAACACCTT CTGTTCACCA CACAAGCGTG GACGCAACGG ATTGCATGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995595.1
CDS	148..4650
Translation	MEGSDFVDPAFSSGERISASDLNSEHIIQAENHSFANRISMDMDVPDGHQLDSNLTGFRWKRVLNPTGPQPRPRHGHRAINIKELMVVFGGGNEGIVDELHVYNTVTNQWYVPVLKGDVPNGCAAYGFVVEGTRMFVFGGMIEYGKYSNELYELQATKWEWRKMYPESPDSGLSPCPRLGHSFTMVGEKIFLFGGLANESDDPKNNIPKYLNDLYILDTRGVHSHNGKWIVPKTYGDSPPPRESHTGISFATKSNGNLNLLIYGGMSGCRLGDLWLLETDSMTWSKPKTSGEAPLPRSLHSSTMIGNKMYVFGGWVPLVINDSKSTTEREWKCTNTLAVLDLETMTWENVTLDTVEENVPRARAGHCAVGIQSRLYVWSGRDGYRKAWNNQVCCKDLWYLEVSKPLYAVKVALVRASTHALELSWTATTFAAAYVLQIQKIEQPLNTSSKLLSNNIVQQGTPTSAETSGINISANRSGSALGLGVEATSTVLKLEKESLQLSGCQPETNVQPSVNDLLQSMSQPSSPASRADKDPLSSGGGTTFNLSTSVASVHPQISVISSTAAVTGNDTASPSGAINSILQKFRPVVTAVRTSTTTAVSIATSTSDPLSVRVPSTMSANVVLSSSSSTLRIVPSVTASHSLRIASSQASGNNCRSSSAINILKTALPNVAVQSQPTSSTTTSIGGKQYFIQKPLTLAPNVQLQFVKTSGGMTVQTLPKVNFTASKGTPPHGISIANPHLASGITQIQGSTVPGSQIQKPIVSGNVLKLVSPHTMAGGKLIMKNSNILQMGKVTPNVMGGKPAFVITNKQGTPLGNQQIIIVTTGGNVRSVPTSTVMTSAGGSASGTNIVSIVNSTSTTPSPLQALSGQKTLISNQSGVKMLRNISSVQASSSMAFGQKQSGTPIHQKTALYIGGKAVTVMSTNTSMAASGNKVMVLPGTSSNNSPATTTALSARKSFVFNAGGSPRTVTLATKSINAKSIPQSQPVTETNNHSVATIKDTDPMDDIIEQLDGAGDLLKLSESEGQHGSEENENNGENATSSSASALFTGGDTAGPSRAQNPIVMEHPVDIIEDVSGVSSTTDVNETAIVSGDTIESLKMSEKENDDVKSMGEKSILSDDCHQPTTSETEAATILTTIKSAEALVLETAEIRKDHTGCTIGSLKENQDENKKFKQRQESSPSQNIHQFQNVDGSQLEALASAALLQAATSDTTALALKELIERPESETNTRSSNIAEIQQNNVQSTLAVVVPNTSQNENQKWHTVGVFKDLSHTVTSYIDSNCISDSFFDGIDVDNLPDFSKFPRTNLEPGTAYRFRLSAINSCGRGEWGEISSFKTCLPGFPGAPSAIKISKDVKEGAHLTWEPPPAQKTKEIIEYSVYLAVKPTAKDKALSTPQLAFVRVYVGAANQCTVPNASLSNAHVDCSNKPAIIFRIAARNQKGYGPATQVRWLQDPAAAKQHTPTVTPNLKRGPEKSTIGSSNIANTFCSPHKRGRNGLHD

Target ORF information:

RefSeq Version	NM_205873.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster host cell factor, transcript variant G (Hcf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205873.3

ORF Insert Sequence:

ATGGAAGGCT CAGACTTTGT GGATCCAGCC TTTTCTTCAG GAGAACGAAT ATCGGCATCT 
GATCTCAATT CGGAGCATAT TATTCAGGCC GAGAATCACA GTTTTGCTAA TCGAATTTCT 
ATGGATATGG ATGTCCCTGA TGGGCACCAG CTGGACAGCA ACTTAACCGG ATTTCGATGG 
AAGCGAGTTC TTAACCCAAC CGGACCGCAG CCCCGCCCGA GACATGGACA CCGTGCGATA 
AACATCAAAG AGCTAATGGT CGTCTTCGGC GGCGGAAACG AAGGAATTGT TGACGAGTTG 
CACGTTTATA ATACTGTTAC TAATCAGTGG TACGTCCCAG TGCTAAAAGG TGATGTACCG 
AACGGATGCG CTGCTTATGG ATTTGTTGTG GAGGGTACTC GCATGTTTGT TTTTGGGGGA 
ATGATCGAAT ACGGAAAATA TTCAAACGAG CTCTATGAGC TACAGGCAAC TAAATGGGAA 
TGGAGAAAAA TGTATCCAGA GTCACCAGAC AGTGGGTTGT CACCGTGTCC TCGCTTGGGT 
CACAGCTTTA CAATGGTTGG TGAAAAAATA TTTCTTTTCG GCGGACTAGC AAATGAATCG 
GACGACCCAA AAAATAATAT TCCAAAATAC TTAAACGACT TGTACATACT GGATACTCGA 
GGAGTTCACA GTCATAACGG AAAGTGGATA GTACCTAAAA CATATGGGGA TAGCCCGCCA 
CCACGAGAGT CGCACACAGG CATATCGTTT GCTACTAAAA GCAACGGAAA TTTAAATCTT 
TTGATTTATG GTGGAATGAG CGGCTGCCGC TTGGGAGATT TATGGTTGCT AGAAACAGAC 
TCCATGACTT GGTCGAAGCC GAAAACTTCA GGGGAGGCGC CGCTACCACG CTCGTTGCAC 
AGTTCTACAA TGATTGGCAA CAAAATGTAT GTCTTTGGTG GCTGGGTTCC CTTAGTGATC 
AATGATTCTA AATCGACAAC AGAGCGTGAA TGGAAATGTA CAAACACTCT TGCTGTCCTT 
GACCTTGAAA CAATGACATG GGAAAATGTC ACATTGGACA CTGTAGAGGA AAATGTTCCA 
CGTGCCCGTG CTGGCCACTG TGCAGTTGGT ATTCAAAGTC GTCTTTATGT GTGGTCAGGT 
CGTGATGGTT ACCGCAAAGC ATGGAACAAC CAGGTTTGCT GCAAAGACCT GTGGTACTTA 
GAGGTCTCCA AGCCCCTGTA TGCAGTAAAA GTTGCGCTAG TTCGCGCTTC TACTCACGCC 
TTGGAGCTCT CGTGGACTGC GACCACTTTT GCAGCAGCCT ACGTTTTGCA AATCCAAAAA 
ATTGAGCAGC CATTAAATAC AAGCTCGAAA CTGTTAAGCA ACAACATTGT TCAACAAGGA 
ACCCCGACAT CTGCTGAAAC TTCTGGAATT AATATTTCAG CAAATAGATC TGGGAGTGCT 
TTGGGTCTTG GTGTCGAAGC CACATCTACA GTCTTAAAAC TAGAAAAGGA ATCTTTGCAA 
CTGAGTGGAT GTCAACCGGA AACAAATGTT CAACCATCTG TAAATGACTT GTTGCAATCA 
ATGTCGCAAC CTTCTTCCCC AGCGTCCCGT GCTGATAAAG ACCCACTTTC GTCAGGGGGT 
GGAACTACCT TTAACTTATC AACATCGGTC GCTTCAGTGC ATCCTCAAAT ATCTGTAATC 
AGTAGTACAG CCGCAGTAAC GGGCAACGAC ACAGCGTCCC CAAGTGGCGC CATTAATAGT 
ATATTGCAAA AGTTTCGACC AGTTGTTACC GCTGTAAGAA CGTCCACAAC TACTGCGGTA 
TCTATTGCTA CCAGCACGTC TGATCCCTTG TCTGTGCGAG TACCAAGTAC GATGTCGGCA 
AACGTTGTTC TCAGCTCTTC AAGTAGTACA TTGCGTATTG TCCCAAGCGT AACTGCATCG 
CATTCACTAC GTATAGCTTC TTCTCAAGCA AGTGGCAACA ACTGCCGGAG TAGCTCTGCA 
ATAAATATAT TAAAAACAGC TCTTCCAAAC GTCGCCGTGC AATCTCAGCC TACATCATCG 
ACCACCACCT CCATTGGTGG AAAACAGTAC TTCATTCAAA AGCCACTTAC ACTTGCTCCA 
AATGTTCAGC TCCAATTTGT TAAGACCAGT GGTGGGATGA CTGTTCAAAC TCTTCCAAAA 
GTAAACTTTA CGGCTTCAAA AGGAACGCCC CCACATGGCA TATCAATTGC AAACCCACAT 
TTGGCGTCTG GTATTACTCA AATTCAGGGA TCGACAGTGC CTGGAAGTCA GATTCAAAAG 
CCCATTGTGT CCGGAAACGT TTTAAAGCTT GTATCGCCGC ATACGATGGC CGGTGGAAAA 
TTGATTATGA AGAATTCAAA CATCTTACAG ATGGGCAAGG TAACTCCGAA CGTAATGGGT 
GGAAAACCAG CTTTTGTCAT AACTAATAAA CAGGGTACGC CATTGGGAAA TCAGCAGATT 
ATTATTGTAA CCACTGGTGG CAATGTGCGA TCTGTTCCAA CTAGCACAGT TATGACAAGT 
GCTGGTGGCT CTGCATCAGG GACAAATATT GTAAGTATAG TTAACTCAAC GTCGACCACG 
CCTAGCCCGC TGCAGGCATT AAGTGGACAA AAAACTTTAA TATCCAACCA AAGTGGAGTT 
AAGATGCTGC GAAATATATC ATCAGTACAA GCATCATCTT CAATGGCATT TGGGCAAAAA 
CAAAGTGGCA CTCCCATTCA TCAAAAAACG GCATTGTATA TAGGAGGCAA AGCTGTTACC 
GTTATGAGCA CCAATACAAG TATGGCTGCA TCCGGAAATA AAGTTATGGT ACTACCAGGA 
ACAAGCTCAA ATAATTCTCC TGCTACTACA ACGGCGCTGA GTGCCAGAAA AAGTTTTGTT 
TTCAATGCCG GAGGTAGCCC TCGGACCGTC ACTTTAGCAA CTAAAAGCAT TAATGCAAAA 
TCTATACCAC AATCGCAACC AGTGACGGAA ACTAATAACC ATTCAGTCGC TACTATTAAA 
GACACAGATC CAATGGATGA TATTATAGAG CAGTTGGATG GAGCCGGAGA TCTGCTAAAG 
CTTTCTGAAA GTGAAGGCCA GCATGGATCG GAGGAGAATG AAAATAATGG TGAAAACGCA 
ACGTCTTCAT CTGCCTCTGC CCTGTTTACC GGAGGTGATA CGGCCGGGCC ATCGAGAGCC 
CAAAACCCTA TAGTAATGGA ACATCCGGTT GATATTATAG AAGATGTCTC TGGCGTGAGC 
AGCACAACTG ACGTTAACGA AACTGCAATC GTAAGTGGTG ATACAATAGA ATCGCTTAAG 
ATGTCGGAGA AGGAAAACGA TGATGTGAAA TCTATGGGTG AAAAATCAAT ATTATCCGAT 
GATTGTCATC AGCCGACGAC TTCCGAGACG GAAGCGGCCA CCATCCTAAC CACAATTAAG 
TCTGCAGAGG CTTTAGTGTT GGAAACTGCA GAAATAAGGA AAGATCATAC CGGATGTACT 
ATAGGTAGTT TGAAAGAAAA TCAAGATGAA AATAAAAAGT TTAAACAACG GCAAGAATCT 
TCACCGTCTC AAAACATTCA CCAATTCCAA AATGTGGATG GTTCACAATT AGAGGCTCTT 
GCTTCTGCTG CGTTACTGCA AGCTGCAACA TCAGACACCA CAGCATTGGC GTTGAAAGAA 
TTGATAGAGC GACCGGAAAG TGAAACAAAT ACCAGAAGTA GCAACATTGC TGAGATTCAA 
CAAAATAATG TTCAATCCAC CTTAGCGGTC GTGGTTCCAA ACACCTCCCA AAACGAGAAC 
CAAAAATGGC ACACAGTAGG TGTGTTTAAG GATCTTTCTC ACACAGTGAC TAGCTACATT 
GATTCGAATT GTATTAGTGA TTCTTTTTTT GACGGTATAG ATGTGGATAA TCTTCCAGAT 
TTCAGCAAGT TTCCACGCAC CAATTTGGAG CCTGGAACGG CTTACCGTTT TCGACTGAGT 
GCTATCAATT CTTGCGGACG AGGAGAATGG GGAGAGATAT CAAGTTTTAA AACCTGTTTA 
CCAGGCTTTC CGGGTGCCCC CTCAGCTATT AAAATTTCCA AGGATGTCAA GGAGGGCGCT 
CACTTAACAT GGGAACCACC ACCAGCTCAA AAAACTAAGG AGATAATTGA ATATTCAGTA 
TACCTTGCAG TTAAGCCTAC TGCCAAGGAT AAGGCGTTGT CCACTCCACA ATTAGCTTTT 
GTGCGGGTTT ATGTAGGTGC GGCAAATCAA TGCACAGTGC CGAATGCTTC ACTATCTAAT 
GCACATGTGG ATTGCTCAAA TAAACCAGCA ATTATATTCC GAATCGCTGC TCGCAATCAA 
AAGGGCTATG GACCTGCCAC CCAAGTTAGA TGGCTGCAGG ATCCCGCGGC AGCGAAACAG 
CACACACCCA CAGTTACGCC AAATCTAAAA CGTGGACCAG AAAAATCAAC TATTGGAAGC 
AGCAACATAG CAAACACCTT CTGTTCACCA CACAAGCGTG GACGCAACGG ATTGCATGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcf ( NM_079882.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524621.2 Drosophila melanogaster host cell factor, transcript variant A (Hcf), mRNA.

Price & Availability↑

CloneID	OFa00262
Clone ID Related Accession (Same CDS sequence)	NM_205873.3 , NM_079882.4 , NM_166756.3
Accession Version	NM_079882.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	host cell factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_079882.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGGCT CAGACTTTGT GGATCCAGCC TTTTCTTCAG GAGAACGAAT ATCGGCATCT 
GATCTCAATT CGGAGCATAT TATTCAGGCC GAGAATCACA GTTTTGCTAA TCGAATTTCT 
ATGGATATGG ATGTCCCTGA TGGGCACCAG CTGGACAGCA ACTTAACCGG ATTTCGATGG 
AAGCGAGTTC TTAACCCAAC CGGACCGCAG CCCCGCCCGA GACATGGACA CCGTGCGATA 
AACATCAAAG AGCTAATGGT CGTCTTCGGC GGCGGAAACG AAGGAATTGT TGACGAGTTG 
CACGTTTATA ATACTGTTAC TAATCAGTGG TACGTCCCAG TGCTAAAAGG TGATGTACCG 
AACGGATGCG CTGCTTATGG ATTTGTTGTG GAGGGTACTC GCATGTTTGT TTTTGGGGGA 
ATGATCGAAT ACGGAAAATA TTCAAACGAG CTCTATGAGC TACAGGCAAC TAAATGGGAA 
TGGAGAAAAA TGTATCCAGA GTCACCAGAC AGTGGGTTGT CACCGTGTCC TCGCTTGGGT 
CACAGCTTTA CAATGGTTGG TGAAAAAATA TTTCTTTTCG GCGGACTAGC AAATGAATCG 
GACGACCCAA AAAATAATAT TCCAAAATAC TTAAACGACT TGTACATACT GGATACTCGA 
GGAGTTCACA GTCATAACGG AAAGTGGATA GTACCTAAAA CATATGGGGA TAGCCCGCCA 
CCACGAGAGT CGCACACAGG CATATCGTTT GCTACTAAAA GCAACGGAAA TTTAAATCTT 
TTGATTTATG GTGGAATGAG CGGCTGCCGC TTGGGAGATT TATGGTTGCT AGAAACAGAC 
TCCATGACTT GGTCGAAGCC GAAAACTTCA GGGGAGGCGC CGCTACCACG CTCGTTGCAC 
AGTTCTACAA TGATTGGCAA CAAAATGTAT GTCTTTGGTG GCTGGGTTCC CTTAGTGATC 
AATGATTCTA AATCGACAAC AGAGCGTGAA TGGAAATGTA CAAACACTCT TGCTGTCCTT 
GACCTTGAAA CAATGACATG GGAAAATGTC ACATTGGACA CTGTAGAGGA AAATGTTCCA 
CGTGCCCGTG CTGGCCACTG TGCAGTTGGT ATTCAAAGTC GTCTTTATGT GTGGTCAGGT 
CGTGATGGTT ACCGCAAAGC ATGGAACAAC CAGGTTTGCT GCAAAGACCT GTGGTACTTA 
GAGGTCTCCA AGCCCCTGTA TGCAGTAAAA GTTGCGCTAG TTCGCGCTTC TACTCACGCC 
TTGGAGCTCT CGTGGACTGC GACCACTTTT GCAGCAGCCT ACGTTTTGCA AATCCAAAAA 
ATTGAGCAGC CATTAAATAC AAGCTCGAAA CTGTTAAGCA ACAACATTGT TCAACAAGGA 
ACCCCGACAT CTGCTGAAAC TTCTGGAATT AATATTTCAG CAAATAGATC TGGGAGTGCT 
TTGGGTCTTG GTGTCGAAGC CACATCTACA GTCTTAAAAC TAGAAAAGGA ATCTTTGCAA 
CTGAGTGGAT GTCAACCGGA AACAAATGTT CAACCATCTG TAAATGACTT GTTGCAATCA 
ATGTCGCAAC CTTCTTCCCC AGCGTCCCGT GCTGATAAAG ACCCACTTTC GTCAGGGGGT 
GGAACTACCT TTAACTTATC AACATCGGTC GCTTCAGTGC ATCCTCAAAT ATCTGTAATC 
AGTAGTACAG CCGCAGTAAC GGGCAACGAC ACAGCGTCCC CAAGTGGCGC CATTAATAGT 
ATATTGCAAA AGTTTCGACC AGTTGTTACC GCTGTAAGAA CGTCCACAAC TACTGCGGTA 
TCTATTGCTA CCAGCACGTC TGATCCCTTG TCTGTGCGAG TACCAAGTAC GATGTCGGCA 
AACGTTGTTC TCAGCTCTTC AAGTAGTACA TTGCGTATTG TCCCAAGCGT AACTGCATCG 
CATTCACTAC GTATAGCTTC TTCTCAAGCA AGTGGCAACA ACTGCCGGAG TAGCTCTGCA 
ATAAATATAT TAAAAACAGC TCTTCCAAAC GTCGCCGTGC AATCTCAGCC TACATCATCG 
ACCACCACCT CCATTGGTGG AAAACAGTAC TTCATTCAAA AGCCACTTAC ACTTGCTCCA 
AATGTTCAGC TCCAATTTGT TAAGACCAGT GGTGGGATGA CTGTTCAAAC TCTTCCAAAA 
GTAAACTTTA CGGCTTCAAA AGGAACGCCC CCACATGGCA TATCAATTGC AAACCCACAT 
TTGGCGTCTG GTATTACTCA AATTCAGGGA TCGACAGTGC CTGGAAGTCA GATTCAAAAG 
CCCATTGTGT CCGGAAACGT TTTAAAGCTT GTATCGCCGC ATACGATGGC CGGTGGAAAA 
TTGATTATGA AGAATTCAAA CATCTTACAG ATGGGCAAGG TAACTCCGAA CGTAATGGGT 
GGAAAACCAG CTTTTGTCAT AACTAATAAA CAGGGTACGC CATTGGGAAA TCAGCAGATT 
ATTATTGTAA CCACTGGTGG CAATGTGCGA TCTGTTCCAA CTAGCACAGT TATGACAAGT 
GCTGGTGGCT CTGCATCAGG GACAAATATT GTAAGTATAG TTAACTCAAC GTCGACCACG 
CCTAGCCCGC TGCAGGCATT AAGTGGACAA AAAACTTTAA TATCCAACCA AAGTGGAGTT 
AAGATGCTGC GAAATATATC ATCAGTACAA GCATCATCTT CAATGGCATT TGGGCAAAAA 
CAAAGTGGCA CTCCCATTCA TCAAAAAACG GCATTGTATA TAGGAGGCAA AGCTGTTACC 
GTTATGAGCA CCAATACAAG TATGGCTGCA TCCGGAAATA AAGTTATGGT ACTACCAGGA 
ACAAGCTCAA ATAATTCTCC TGCTACTACA ACGGCGCTGA GTGCCAGAAA AAGTTTTGTT 
TTCAATGCCG GAGGTAGCCC TCGGACCGTC ACTTTAGCAA CTAAAAGCAT TAATGCAAAA 
TCTATACCAC AATCGCAACC AGTGACGGAA ACTAATAACC ATTCAGTCGC TACTATTAAA 
GACACAGATC CAATGGATGA TATTATAGAG CAGTTGGATG GAGCCGGAGA TCTGCTAAAG 
CTTTCTGAAA GTGAAGGCCA GCATGGATCG GAGGAGAATG AAAATAATGG TGAAAACGCA 
ACGTCTTCAT CTGCCTCTGC CCTGTTTACC GGAGGTGATA CGGCCGGGCC ATCGAGAGCC 
CAAAACCCTA TAGTAATGGA ACATCCGGTT GATATTATAG AAGATGTCTC TGGCGTGAGC 
AGCACAACTG ACGTTAACGA AACTGCAATC GTAAGTGGTG ATACAATAGA ATCGCTTAAG 
ATGTCGGAGA AGGAAAACGA TGATGTGAAA TCTATGGGTG AAAAATCAAT ATTATCCGAT 
GATTGTCATC AGCCGACGAC TTCCGAGACG GAAGCGGCCA CCATCCTAAC CACAATTAAG 
TCTGCAGAGG CTTTAGTGTT GGAAACTGCA GAAATAAGGA AAGATCATAC CGGATGTACT 
ATAGGTAGTT TGAAAGAAAA TCAAGATGAA AATAAAAAGT TTAAACAACG GCAAGAATCT 
TCACCGTCTC AAAACATTCA CCAATTCCAA AATGTGGATG GTTCACAATT AGAGGCTCTT 
GCTTCTGCTG CGTTACTGCA AGCTGCAACA TCAGACACCA CAGCATTGGC GTTGAAAGAA 
TTGATAGAGC GACCGGAAAG TGAAACAAAT ACCAGAAGTA GCAACATTGC TGAGATTCAA 
CAAAATAATG TTCAATCCAC CTTAGCGGTC GTGGTTCCAA ACACCTCCCA AAACGAGAAC 
CAAAAATGGC ACACAGTAGG TGTGTTTAAG GATCTTTCTC ACACAGTGAC TAGCTACATT 
GATTCGAATT GTATTAGTGA TTCTTTTTTT GACGGTATAG ATGTGGATAA TCTTCCAGAT 
TTCAGCAAGT TTCCACGCAC CAATTTGGAG CCTGGAACGG CTTACCGTTT TCGACTGAGT 
GCTATCAATT CTTGCGGACG AGGAGAATGG GGAGAGATAT CAAGTTTTAA AACCTGTTTA 
CCAGGCTTTC CGGGTGCCCC CTCAGCTATT AAAATTTCCA AGGATGTCAA GGAGGGCGCT 
CACTTAACAT GGGAACCACC ACCAGCTCAA AAAACTAAGG AGATAATTGA ATATTCAGTA 
TACCTTGCAG TTAAGCCTAC TGCCAAGGAT AAGGCGTTGT CCACTCCACA ATTAGCTTTT 
GTGCGGGTTT ATGTAGGTGC GGCAAATCAA TGCACAGTGC CGAATGCTTC ACTATCTAAT 
GCACATGTGG ATTGCTCAAA TAAACCAGCA ATTATATTCC GAATCGCTGC TCGCAATCAA 
AAGGGCTATG GACCTGCCAC CCAAGTTAGA TGGCTGCAGG ATCCCGCGGC AGCGAAACAG 
CACACACCCA CAGTTACGCC AAATCTAAAA CGTGGACCAG AAAAATCAAC TATTGGAAGC 
AGCAACATAG CAAACACCTT CTGTTCACCA CACAAGCGTG GACGCAACGG ATTGCATGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524621.2
CDS	452..4954
Translation	MEGSDFVDPAFSSGERISASDLNSEHIIQAENHSFANRISMDMDVPDGHQLDSNLTGFRWKRVLNPTGPQPRPRHGHRAINIKELMVVFGGGNEGIVDELHVYNTVTNQWYVPVLKGDVPNGCAAYGFVVEGTRMFVFGGMIEYGKYSNELYELQATKWEWRKMYPESPDSGLSPCPRLGHSFTMVGEKIFLFGGLANESDDPKNNIPKYLNDLYILDTRGVHSHNGKWIVPKTYGDSPPPRESHTGISFATKSNGNLNLLIYGGMSGCRLGDLWLLETDSMTWSKPKTSGEAPLPRSLHSSTMIGNKMYVFGGWVPLVINDSKSTTEREWKCTNTLAVLDLETMTWENVTLDTVEENVPRARAGHCAVGIQSRLYVWSGRDGYRKAWNNQVCCKDLWYLEVSKPLYAVKVALVRASTHALELSWTATTFAAAYVLQIQKIEQPLNTSSKLLSNNIVQQGTPTSAETSGINISANRSGSALGLGVEATSTVLKLEKESLQLSGCQPETNVQPSVNDLLQSMSQPSSPASRADKDPLSSGGGTTFNLSTSVASVHPQISVISSTAAVTGNDTASPSGAINSILQKFRPVVTAVRTSTTTAVSIATSTSDPLSVRVPSTMSANVVLSSSSSTLRIVPSVTASHSLRIASSQASGNNCRSSSAINILKTALPNVAVQSQPTSSTTTSIGGKQYFIQKPLTLAPNVQLQFVKTSGGMTVQTLPKVNFTASKGTPPHGISIANPHLASGITQIQGSTVPGSQIQKPIVSGNVLKLVSPHTMAGGKLIMKNSNILQMGKVTPNVMGGKPAFVITNKQGTPLGNQQIIIVTTGGNVRSVPTSTVMTSAGGSASGTNIVSIVNSTSTTPSPLQALSGQKTLISNQSGVKMLRNISSVQASSSMAFGQKQSGTPIHQKTALYIGGKAVTVMSTNTSMAASGNKVMVLPGTSSNNSPATTTALSARKSFVFNAGGSPRTVTLATKSINAKSIPQSQPVTETNNHSVATIKDTDPMDDIIEQLDGAGDLLKLSESEGQHGSEENENNGENATSSSASALFTGGDTAGPSRAQNPIVMEHPVDIIEDVSGVSSTTDVNETAIVSGDTIESLKMSEKENDDVKSMGEKSILSDDCHQPTTSETEAATILTTIKSAEALVLETAEIRKDHTGCTIGSLKENQDENKKFKQRQESSPSQNIHQFQNVDGSQLEALASAALLQAATSDTTALALKELIERPESETNTRSSNIAEIQQNNVQSTLAVVVPNTSQNENQKWHTVGVFKDLSHTVTSYIDSNCISDSFFDGIDVDNLPDFSKFPRTNLEPGTAYRFRLSAINSCGRGEWGEISSFKTCLPGFPGAPSAIKISKDVKEGAHLTWEPPPAQKTKEIIEYSVYLAVKPTAKDKALSTPQLAFVRVYVGAANQCTVPNASLSNAHVDCSNKPAIIFRIAARNQKGYGPATQVRWLQDPAAAKQHTPTVTPNLKRGPEKSTIGSSNIANTFCSPHKRGRNGLHD

Target ORF information:

RefSeq Version	NM_079882.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster host cell factor, transcript variant A (Hcf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079882.4

ORF Insert Sequence:

ATGGAAGGCT CAGACTTTGT GGATCCAGCC TTTTCTTCAG GAGAACGAAT ATCGGCATCT 
GATCTCAATT CGGAGCATAT TATTCAGGCC GAGAATCACA GTTTTGCTAA TCGAATTTCT 
ATGGATATGG ATGTCCCTGA TGGGCACCAG CTGGACAGCA ACTTAACCGG ATTTCGATGG 
AAGCGAGTTC TTAACCCAAC CGGACCGCAG CCCCGCCCGA GACATGGACA CCGTGCGATA 
AACATCAAAG AGCTAATGGT CGTCTTCGGC GGCGGAAACG AAGGAATTGT TGACGAGTTG 
CACGTTTATA ATACTGTTAC TAATCAGTGG TACGTCCCAG TGCTAAAAGG TGATGTACCG 
AACGGATGCG CTGCTTATGG ATTTGTTGTG GAGGGTACTC GCATGTTTGT TTTTGGGGGA 
ATGATCGAAT ACGGAAAATA TTCAAACGAG CTCTATGAGC TACAGGCAAC TAAATGGGAA 
TGGAGAAAAA TGTATCCAGA GTCACCAGAC AGTGGGTTGT CACCGTGTCC TCGCTTGGGT 
CACAGCTTTA CAATGGTTGG TGAAAAAATA TTTCTTTTCG GCGGACTAGC AAATGAATCG 
GACGACCCAA AAAATAATAT TCCAAAATAC TTAAACGACT TGTACATACT GGATACTCGA 
GGAGTTCACA GTCATAACGG AAAGTGGATA GTACCTAAAA CATATGGGGA TAGCCCGCCA 
CCACGAGAGT CGCACACAGG CATATCGTTT GCTACTAAAA GCAACGGAAA TTTAAATCTT 
TTGATTTATG GTGGAATGAG CGGCTGCCGC TTGGGAGATT TATGGTTGCT AGAAACAGAC 
TCCATGACTT GGTCGAAGCC GAAAACTTCA GGGGAGGCGC CGCTACCACG CTCGTTGCAC 
AGTTCTACAA TGATTGGCAA CAAAATGTAT GTCTTTGGTG GCTGGGTTCC CTTAGTGATC 
AATGATTCTA AATCGACAAC AGAGCGTGAA TGGAAATGTA CAAACACTCT TGCTGTCCTT 
GACCTTGAAA CAATGACATG GGAAAATGTC ACATTGGACA CTGTAGAGGA AAATGTTCCA 
CGTGCCCGTG CTGGCCACTG TGCAGTTGGT ATTCAAAGTC GTCTTTATGT GTGGTCAGGT 
CGTGATGGTT ACCGCAAAGC ATGGAACAAC CAGGTTTGCT GCAAAGACCT GTGGTACTTA 
GAGGTCTCCA AGCCCCTGTA TGCAGTAAAA GTTGCGCTAG TTCGCGCTTC TACTCACGCC 
TTGGAGCTCT CGTGGACTGC GACCACTTTT GCAGCAGCCT ACGTTTTGCA AATCCAAAAA 
ATTGAGCAGC CATTAAATAC AAGCTCGAAA CTGTTAAGCA ACAACATTGT TCAACAAGGA 
ACCCCGACAT CTGCTGAAAC TTCTGGAATT AATATTTCAG CAAATAGATC TGGGAGTGCT 
TTGGGTCTTG GTGTCGAAGC CACATCTACA GTCTTAAAAC TAGAAAAGGA ATCTTTGCAA 
CTGAGTGGAT GTCAACCGGA AACAAATGTT CAACCATCTG TAAATGACTT GTTGCAATCA 
ATGTCGCAAC CTTCTTCCCC AGCGTCCCGT GCTGATAAAG ACCCACTTTC GTCAGGGGGT 
GGAACTACCT TTAACTTATC AACATCGGTC GCTTCAGTGC ATCCTCAAAT ATCTGTAATC 
AGTAGTACAG CCGCAGTAAC GGGCAACGAC ACAGCGTCCC CAAGTGGCGC CATTAATAGT 
ATATTGCAAA AGTTTCGACC AGTTGTTACC GCTGTAAGAA CGTCCACAAC TACTGCGGTA 
TCTATTGCTA CCAGCACGTC TGATCCCTTG TCTGTGCGAG TACCAAGTAC GATGTCGGCA 
AACGTTGTTC TCAGCTCTTC AAGTAGTACA TTGCGTATTG TCCCAAGCGT AACTGCATCG 
CATTCACTAC GTATAGCTTC TTCTCAAGCA AGTGGCAACA ACTGCCGGAG TAGCTCTGCA 
ATAAATATAT TAAAAACAGC TCTTCCAAAC GTCGCCGTGC AATCTCAGCC TACATCATCG 
ACCACCACCT CCATTGGTGG AAAACAGTAC TTCATTCAAA AGCCACTTAC ACTTGCTCCA 
AATGTTCAGC TCCAATTTGT TAAGACCAGT GGTGGGATGA CTGTTCAAAC TCTTCCAAAA 
GTAAACTTTA CGGCTTCAAA AGGAACGCCC CCACATGGCA TATCAATTGC AAACCCACAT 
TTGGCGTCTG GTATTACTCA AATTCAGGGA TCGACAGTGC CTGGAAGTCA GATTCAAAAG 
CCCATTGTGT CCGGAAACGT TTTAAAGCTT GTATCGCCGC ATACGATGGC CGGTGGAAAA 
TTGATTATGA AGAATTCAAA CATCTTACAG ATGGGCAAGG TAACTCCGAA CGTAATGGGT 
GGAAAACCAG CTTTTGTCAT AACTAATAAA CAGGGTACGC CATTGGGAAA TCAGCAGATT 
ATTATTGTAA CCACTGGTGG CAATGTGCGA TCTGTTCCAA CTAGCACAGT TATGACAAGT 
GCTGGTGGCT CTGCATCAGG GACAAATATT GTAAGTATAG TTAACTCAAC GTCGACCACG 
CCTAGCCCGC TGCAGGCATT AAGTGGACAA AAAACTTTAA TATCCAACCA AAGTGGAGTT 
AAGATGCTGC GAAATATATC ATCAGTACAA GCATCATCTT CAATGGCATT TGGGCAAAAA 
CAAAGTGGCA CTCCCATTCA TCAAAAAACG GCATTGTATA TAGGAGGCAA AGCTGTTACC 
GTTATGAGCA CCAATACAAG TATGGCTGCA TCCGGAAATA AAGTTATGGT ACTACCAGGA 
ACAAGCTCAA ATAATTCTCC TGCTACTACA ACGGCGCTGA GTGCCAGAAA AAGTTTTGTT 
TTCAATGCCG GAGGTAGCCC TCGGACCGTC ACTTTAGCAA CTAAAAGCAT TAATGCAAAA 
TCTATACCAC AATCGCAACC AGTGACGGAA ACTAATAACC ATTCAGTCGC TACTATTAAA 
GACACAGATC CAATGGATGA TATTATAGAG CAGTTGGATG GAGCCGGAGA TCTGCTAAAG 
CTTTCTGAAA GTGAAGGCCA GCATGGATCG GAGGAGAATG AAAATAATGG TGAAAACGCA 
ACGTCTTCAT CTGCCTCTGC CCTGTTTACC GGAGGTGATA CGGCCGGGCC ATCGAGAGCC 
CAAAACCCTA TAGTAATGGA ACATCCGGTT GATATTATAG AAGATGTCTC TGGCGTGAGC 
AGCACAACTG ACGTTAACGA AACTGCAATC GTAAGTGGTG ATACAATAGA ATCGCTTAAG 
ATGTCGGAGA AGGAAAACGA TGATGTGAAA TCTATGGGTG AAAAATCAAT ATTATCCGAT 
GATTGTCATC AGCCGACGAC TTCCGAGACG GAAGCGGCCA CCATCCTAAC CACAATTAAG 
TCTGCAGAGG CTTTAGTGTT GGAAACTGCA GAAATAAGGA AAGATCATAC CGGATGTACT 
ATAGGTAGTT TGAAAGAAAA TCAAGATGAA AATAAAAAGT TTAAACAACG GCAAGAATCT 
TCACCGTCTC AAAACATTCA CCAATTCCAA AATGTGGATG GTTCACAATT AGAGGCTCTT 
GCTTCTGCTG CGTTACTGCA AGCTGCAACA TCAGACACCA CAGCATTGGC GTTGAAAGAA 
TTGATAGAGC GACCGGAAAG TGAAACAAAT ACCAGAAGTA GCAACATTGC TGAGATTCAA 
CAAAATAATG TTCAATCCAC CTTAGCGGTC GTGGTTCCAA ACACCTCCCA AAACGAGAAC 
CAAAAATGGC ACACAGTAGG TGTGTTTAAG GATCTTTCTC ACACAGTGAC TAGCTACATT 
GATTCGAATT GTATTAGTGA TTCTTTTTTT GACGGTATAG ATGTGGATAA TCTTCCAGAT 
TTCAGCAAGT TTCCACGCAC CAATTTGGAG CCTGGAACGG CTTACCGTTT TCGACTGAGT 
GCTATCAATT CTTGCGGACG AGGAGAATGG GGAGAGATAT CAAGTTTTAA AACCTGTTTA 
CCAGGCTTTC CGGGTGCCCC CTCAGCTATT AAAATTTCCA AGGATGTCAA GGAGGGCGCT 
CACTTAACAT GGGAACCACC ACCAGCTCAA AAAACTAAGG AGATAATTGA ATATTCAGTA 
TACCTTGCAG TTAAGCCTAC TGCCAAGGAT AAGGCGTTGT CCACTCCACA ATTAGCTTTT 
GTGCGGGTTT ATGTAGGTGC GGCAAATCAA TGCACAGTGC CGAATGCTTC ACTATCTAAT 
GCACATGTGG ATTGCTCAAA TAAACCAGCA ATTATATTCC GAATCGCTGC TCGCAATCAA 
AAGGGCTATG GACCTGCCAC CCAAGTTAGA TGGCTGCAGG ATCCCGCGGC AGCGAAACAG 
CACACACCCA CAGTTACGCC AAATCTAAAA CGTGGACCAG AAAAATCAAC TATTGGAAGC 
AGCAACATAG CAAACACCTT CTGTTCACCA CACAAGCGTG GACGCAACGG ATTGCATGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcf ( NM_166756.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726566.1 Drosophila melanogaster host cell factor, transcript variant B (Hcf), mRNA.

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CloneID	OFa00262
Clone ID Related Accession (Same CDS sequence)	NM_205873.3 , NM_079882.4 , NM_166756.3
Accession Version	NM_166756.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	host cell factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166756.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGGCT CAGACTTTGT GGATCCAGCC TTTTCTTCAG GAGAACGAAT ATCGGCATCT 
GATCTCAATT CGGAGCATAT TATTCAGGCC GAGAATCACA GTTTTGCTAA TCGAATTTCT 
ATGGATATGG ATGTCCCTGA TGGGCACCAG CTGGACAGCA ACTTAACCGG ATTTCGATGG 
AAGCGAGTTC TTAACCCAAC CGGACCGCAG CCCCGCCCGA GACATGGACA CCGTGCGATA 
AACATCAAAG AGCTAATGGT CGTCTTCGGC GGCGGAAACG AAGGAATTGT TGACGAGTTG 
CACGTTTATA ATACTGTTAC TAATCAGTGG TACGTCCCAG TGCTAAAAGG TGATGTACCG 
AACGGATGCG CTGCTTATGG ATTTGTTGTG GAGGGTACTC GCATGTTTGT TTTTGGGGGA 
ATGATCGAAT ACGGAAAATA TTCAAACGAG CTCTATGAGC TACAGGCAAC TAAATGGGAA 
TGGAGAAAAA TGTATCCAGA GTCACCAGAC AGTGGGTTGT CACCGTGTCC TCGCTTGGGT 
CACAGCTTTA CAATGGTTGG TGAAAAAATA TTTCTTTTCG GCGGACTAGC AAATGAATCG 
GACGACCCAA AAAATAATAT TCCAAAATAC TTAAACGACT TGTACATACT GGATACTCGA 
GGAGTTCACA GTCATAACGG AAAGTGGATA GTACCTAAAA CATATGGGGA TAGCCCGCCA 
CCACGAGAGT CGCACACAGG CATATCGTTT GCTACTAAAA GCAACGGAAA TTTAAATCTT 
TTGATTTATG GTGGAATGAG CGGCTGCCGC TTGGGAGATT TATGGTTGCT AGAAACAGAC 
TCCATGACTT GGTCGAAGCC GAAAACTTCA GGGGAGGCGC CGCTACCACG CTCGTTGCAC 
AGTTCTACAA TGATTGGCAA CAAAATGTAT GTCTTTGGTG GCTGGGTTCC CTTAGTGATC 
AATGATTCTA AATCGACAAC AGAGCGTGAA TGGAAATGTA CAAACACTCT TGCTGTCCTT 
GACCTTGAAA CAATGACATG GGAAAATGTC ACATTGGACA CTGTAGAGGA AAATGTTCCA 
CGTGCCCGTG CTGGCCACTG TGCAGTTGGT ATTCAAAGTC GTCTTTATGT GTGGTCAGGT 
CGTGATGGTT ACCGCAAAGC ATGGAACAAC CAGGTTTGCT GCAAAGACCT GTGGTACTTA 
GAGGTCTCCA AGCCCCTGTA TGCAGTAAAA GTTGCGCTAG TTCGCGCTTC TACTCACGCC 
TTGGAGCTCT CGTGGACTGC GACCACTTTT GCAGCAGCCT ACGTTTTGCA AATCCAAAAA 
ATTGAGCAGC CATTAAATAC AAGCTCGAAA CTGTTAAGCA ACAACATTGT TCAACAAGGA 
ACCCCGACAT CTGCTGAAAC TTCTGGAATT AATATTTCAG CAAATAGATC TGGGAGTGCT 
TTGGGTCTTG GTGTCGAAGC CACATCTACA GTCTTAAAAC TAGAAAAGGA ATCTTTGCAA 
CTGAGTGGAT GTCAACCGGA AACAAATGTT CAACCATCTG TAAATGACTT GTTGCAATCA 
ATGTCGCAAC CTTCTTCCCC AGCGTCCCGT GCTGATAAAG ACCCACTTTC GTCAGGGGGT 
GGAACTACCT TTAACTTATC AACATCGGTC GCTTCAGTGC ATCCTCAAAT ATCTGTAATC 
AGTAGTACAG CCGCAGTAAC GGGCAACGAC ACAGCGTCCC CAAGTGGCGC CATTAATAGT 
ATATTGCAAA AGTTTCGACC AGTTGTTACC GCTGTAAGAA CGTCCACAAC TACTGCGGTA 
TCTATTGCTA CCAGCACGTC TGATCCCTTG TCTGTGCGAG TACCAAGTAC GATGTCGGCA 
AACGTTGTTC TCAGCTCTTC AAGTAGTACA TTGCGTATTG TCCCAAGCGT AACTGCATCG 
CATTCACTAC GTATAGCTTC TTCTCAAGCA AGTGGCAACA ACTGCCGGAG TAGCTCTGCA 
ATAAATATAT TAAAAACAGC TCTTCCAAAC GTCGCCGTGC AATCTCAGCC TACATCATCG 
ACCACCACCT CCATTGGTGG AAAACAGTAC TTCATTCAAA AGCCACTTAC ACTTGCTCCA 
AATGTTCAGC TCCAATTTGT TAAGACCAGT GGTGGGATGA CTGTTCAAAC TCTTCCAAAA 
GTAAACTTTA CGGCTTCAAA AGGAACGCCC CCACATGGCA TATCAATTGC AAACCCACAT 
TTGGCGTCTG GTATTACTCA AATTCAGGGA TCGACAGTGC CTGGAAGTCA GATTCAAAAG 
CCCATTGTGT CCGGAAACGT TTTAAAGCTT GTATCGCCGC ATACGATGGC CGGTGGAAAA 
TTGATTATGA AGAATTCAAA CATCTTACAG ATGGGCAAGG TAACTCCGAA CGTAATGGGT 
GGAAAACCAG CTTTTGTCAT AACTAATAAA CAGGGTACGC CATTGGGAAA TCAGCAGATT 
ATTATTGTAA CCACTGGTGG CAATGTGCGA TCTGTTCCAA CTAGCACAGT TATGACAAGT 
GCTGGTGGCT CTGCATCAGG GACAAATATT GTAAGTATAG TTAACTCAAC GTCGACCACG 
CCTAGCCCGC TGCAGGCATT AAGTGGACAA AAAACTTTAA TATCCAACCA AAGTGGAGTT 
AAGATGCTGC GAAATATATC ATCAGTACAA GCATCATCTT CAATGGCATT TGGGCAAAAA 
CAAAGTGGCA CTCCCATTCA TCAAAAAACG GCATTGTATA TAGGAGGCAA AGCTGTTACC 
GTTATGAGCA CCAATACAAG TATGGCTGCA TCCGGAAATA AAGTTATGGT ACTACCAGGA 
ACAAGCTCAA ATAATTCTCC TGCTACTACA ACGGCGCTGA GTGCCAGAAA AAGTTTTGTT 
TTCAATGCCG GAGGTAGCCC TCGGACCGTC ACTTTAGCAA CTAAAAGCAT TAATGCAAAA 
TCTATACCAC AATCGCAACC AGTGACGGAA ACTAATAACC ATTCAGTCGC TACTATTAAA 
GACACAGATC CAATGGATGA TATTATAGAG CAGTTGGATG GAGCCGGAGA TCTGCTAAAG 
CTTTCTGAAA GTGAAGGCCA GCATGGATCG GAGGAGAATG AAAATAATGG TGAAAACGCA 
ACGTCTTCAT CTGCCTCTGC CCTGTTTACC GGAGGTGATA CGGCCGGGCC ATCGAGAGCC 
CAAAACCCTA TAGTAATGGA ACATCCGGTT GATATTATAG AAGATGTCTC TGGCGTGAGC 
AGCACAACTG ACGTTAACGA AACTGCAATC GTAAGTGGTG ATACAATAGA ATCGCTTAAG 
ATGTCGGAGA AGGAAAACGA TGATGTGAAA TCTATGGGTG AAAAATCAAT ATTATCCGAT 
GATTGTCATC AGCCGACGAC TTCCGAGACG GAAGCGGCCA CCATCCTAAC CACAATTAAG 
TCTGCAGAGG CTTTAGTGTT GGAAACTGCA GAAATAAGGA AAGATCATAC CGGATGTACT 
ATAGGTAGTT TGAAAGAAAA TCAAGATGAA AATAAAAAGT TTAAACAACG GCAAGAATCT 
TCACCGTCTC AAAACATTCA CCAATTCCAA AATGTGGATG GTTCACAATT AGAGGCTCTT 
GCTTCTGCTG CGTTACTGCA AGCTGCAACA TCAGACACCA CAGCATTGGC GTTGAAAGAA 
TTGATAGAGC GACCGGAAAG TGAAACAAAT ACCAGAAGTA GCAACATTGC TGAGATTCAA 
CAAAATAATG TTCAATCCAC CTTAGCGGTC GTGGTTCCAA ACACCTCCCA AAACGAGAAC 
CAAAAATGGC ACACAGTAGG TGTGTTTAAG GATCTTTCTC ACACAGTGAC TAGCTACATT 
GATTCGAATT GTATTAGTGA TTCTTTTTTT GACGGTATAG ATGTGGATAA TCTTCCAGAT 
TTCAGCAAGT TTCCACGCAC CAATTTGGAG CCTGGAACGG CTTACCGTTT TCGACTGAGT 
GCTATCAATT CTTGCGGACG AGGAGAATGG GGAGAGATAT CAAGTTTTAA AACCTGTTTA 
CCAGGCTTTC CGGGTGCCCC CTCAGCTATT AAAATTTCCA AGGATGTCAA GGAGGGCGCT 
CACTTAACAT GGGAACCACC ACCAGCTCAA AAAACTAAGG AGATAATTGA ATATTCAGTA 
TACCTTGCAG TTAAGCCTAC TGCCAAGGAT AAGGCGTTGT CCACTCCACA ATTAGCTTTT 
GTGCGGGTTT ATGTAGGTGC GGCAAATCAA TGCACAGTGC CGAATGCTTC ACTATCTAAT 
GCACATGTGG ATTGCTCAAA TAAACCAGCA ATTATATTCC GAATCGCTGC TCGCAATCAA 
AAGGGCTATG GACCTGCCAC CCAAGTTAGA TGGCTGCAGG ATCCCGCGGC AGCGAAACAG 
CACACACCCA CAGTTACGCC AAATCTAAAA CGTGGACCAG AAAAATCAAC TATTGGAAGC 
AGCAACATAG CAAACACCTT CTGTTCACCA CACAAGCGTG GACGCAACGG ATTGCATGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726566.1
CDS	148..4650
Translation	MEGSDFVDPAFSSGERISASDLNSEHIIQAENHSFANRISMDMDVPDGHQLDSNLTGFRWKRVLNPTGPQPRPRHGHRAINIKELMVVFGGGNEGIVDELHVYNTVTNQWYVPVLKGDVPNGCAAYGFVVEGTRMFVFGGMIEYGKYSNELYELQATKWEWRKMYPESPDSGLSPCPRLGHSFTMVGEKIFLFGGLANESDDPKNNIPKYLNDLYILDTRGVHSHNGKWIVPKTYGDSPPPRESHTGISFATKSNGNLNLLIYGGMSGCRLGDLWLLETDSMTWSKPKTSGEAPLPRSLHSSTMIGNKMYVFGGWVPLVINDSKSTTEREWKCTNTLAVLDLETMTWENVTLDTVEENVPRARAGHCAVGIQSRLYVWSGRDGYRKAWNNQVCCKDLWYLEVSKPLYAVKVALVRASTHALELSWTATTFAAAYVLQIQKIEQPLNTSSKLLSNNIVQQGTPTSAETSGINISANRSGSALGLGVEATSTVLKLEKESLQLSGCQPETNVQPSVNDLLQSMSQPSSPASRADKDPLSSGGGTTFNLSTSVASVHPQISVISSTAAVTGNDTASPSGAINSILQKFRPVVTAVRTSTTTAVSIATSTSDPLSVRVPSTMSANVVLSSSSSTLRIVPSVTASHSLRIASSQASGNNCRSSSAINILKTALPNVAVQSQPTSSTTTSIGGKQYFIQKPLTLAPNVQLQFVKTSGGMTVQTLPKVNFTASKGTPPHGISIANPHLASGITQIQGSTVPGSQIQKPIVSGNVLKLVSPHTMAGGKLIMKNSNILQMGKVTPNVMGGKPAFVITNKQGTPLGNQQIIIVTTGGNVRSVPTSTVMTSAGGSASGTNIVSIVNSTSTTPSPLQALSGQKTLISNQSGVKMLRNISSVQASSSMAFGQKQSGTPIHQKTALYIGGKAVTVMSTNTSMAASGNKVMVLPGTSSNNSPATTTALSARKSFVFNAGGSPRTVTLATKSINAKSIPQSQPVTETNNHSVATIKDTDPMDDIIEQLDGAGDLLKLSESEGQHGSEENENNGENATSSSASALFTGGDTAGPSRAQNPIVMEHPVDIIEDVSGVSSTTDVNETAIVSGDTIESLKMSEKENDDVKSMGEKSILSDDCHQPTTSETEAATILTTIKSAEALVLETAEIRKDHTGCTIGSLKENQDENKKFKQRQESSPSQNIHQFQNVDGSQLEALASAALLQAATSDTTALALKELIERPESETNTRSSNIAEIQQNNVQSTLAVVVPNTSQNENQKWHTVGVFKDLSHTVTSYIDSNCISDSFFDGIDVDNLPDFSKFPRTNLEPGTAYRFRLSAINSCGRGEWGEISSFKTCLPGFPGAPSAIKISKDVKEGAHLTWEPPPAQKTKEIIEYSVYLAVKPTAKDKALSTPQLAFVRVYVGAANQCTVPNASLSNAHVDCSNKPAIIFRIAARNQKGYGPATQVRWLQDPAAAKQHTPTVTPNLKRGPEKSTIGSSNIANTFCSPHKRGRNGLHD

Target ORF information:

RefSeq Version	NM_166756.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster host cell factor, transcript variant B (Hcf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166756.3

ORF Insert Sequence:

ATGGAAGGCT CAGACTTTGT GGATCCAGCC TTTTCTTCAG GAGAACGAAT ATCGGCATCT 
GATCTCAATT CGGAGCATAT TATTCAGGCC GAGAATCACA GTTTTGCTAA TCGAATTTCT 
ATGGATATGG ATGTCCCTGA TGGGCACCAG CTGGACAGCA ACTTAACCGG ATTTCGATGG 
AAGCGAGTTC TTAACCCAAC CGGACCGCAG CCCCGCCCGA GACATGGACA CCGTGCGATA 
AACATCAAAG AGCTAATGGT CGTCTTCGGC GGCGGAAACG AAGGAATTGT TGACGAGTTG 
CACGTTTATA ATACTGTTAC TAATCAGTGG TACGTCCCAG TGCTAAAAGG TGATGTACCG 
AACGGATGCG CTGCTTATGG ATTTGTTGTG GAGGGTACTC GCATGTTTGT TTTTGGGGGA 
ATGATCGAAT ACGGAAAATA TTCAAACGAG CTCTATGAGC TACAGGCAAC TAAATGGGAA 
TGGAGAAAAA TGTATCCAGA GTCACCAGAC AGTGGGTTGT CACCGTGTCC TCGCTTGGGT 
CACAGCTTTA CAATGGTTGG TGAAAAAATA TTTCTTTTCG GCGGACTAGC AAATGAATCG 
GACGACCCAA AAAATAATAT TCCAAAATAC TTAAACGACT TGTACATACT GGATACTCGA 
GGAGTTCACA GTCATAACGG AAAGTGGATA GTACCTAAAA CATATGGGGA TAGCCCGCCA 
CCACGAGAGT CGCACACAGG CATATCGTTT GCTACTAAAA GCAACGGAAA TTTAAATCTT 
TTGATTTATG GTGGAATGAG CGGCTGCCGC TTGGGAGATT TATGGTTGCT AGAAACAGAC 
TCCATGACTT GGTCGAAGCC GAAAACTTCA GGGGAGGCGC CGCTACCACG CTCGTTGCAC 
AGTTCTACAA TGATTGGCAA CAAAATGTAT GTCTTTGGTG GCTGGGTTCC CTTAGTGATC 
AATGATTCTA AATCGACAAC AGAGCGTGAA TGGAAATGTA CAAACACTCT TGCTGTCCTT 
GACCTTGAAA CAATGACATG GGAAAATGTC ACATTGGACA CTGTAGAGGA AAATGTTCCA 
CGTGCCCGTG CTGGCCACTG TGCAGTTGGT ATTCAAAGTC GTCTTTATGT GTGGTCAGGT 
CGTGATGGTT ACCGCAAAGC ATGGAACAAC CAGGTTTGCT GCAAAGACCT GTGGTACTTA 
GAGGTCTCCA AGCCCCTGTA TGCAGTAAAA GTTGCGCTAG TTCGCGCTTC TACTCACGCC 
TTGGAGCTCT CGTGGACTGC GACCACTTTT GCAGCAGCCT ACGTTTTGCA AATCCAAAAA 
ATTGAGCAGC CATTAAATAC AAGCTCGAAA CTGTTAAGCA ACAACATTGT TCAACAAGGA 
ACCCCGACAT CTGCTGAAAC TTCTGGAATT AATATTTCAG CAAATAGATC TGGGAGTGCT 
TTGGGTCTTG GTGTCGAAGC CACATCTACA GTCTTAAAAC TAGAAAAGGA ATCTTTGCAA 
CTGAGTGGAT GTCAACCGGA AACAAATGTT CAACCATCTG TAAATGACTT GTTGCAATCA 
ATGTCGCAAC CTTCTTCCCC AGCGTCCCGT GCTGATAAAG ACCCACTTTC GTCAGGGGGT 
GGAACTACCT TTAACTTATC AACATCGGTC GCTTCAGTGC ATCCTCAAAT ATCTGTAATC 
AGTAGTACAG CCGCAGTAAC GGGCAACGAC ACAGCGTCCC CAAGTGGCGC CATTAATAGT 
ATATTGCAAA AGTTTCGACC AGTTGTTACC GCTGTAAGAA CGTCCACAAC TACTGCGGTA 
TCTATTGCTA CCAGCACGTC TGATCCCTTG TCTGTGCGAG TACCAAGTAC GATGTCGGCA 
AACGTTGTTC TCAGCTCTTC AAGTAGTACA TTGCGTATTG TCCCAAGCGT AACTGCATCG 
CATTCACTAC GTATAGCTTC TTCTCAAGCA AGTGGCAACA ACTGCCGGAG TAGCTCTGCA 
ATAAATATAT TAAAAACAGC TCTTCCAAAC GTCGCCGTGC AATCTCAGCC TACATCATCG 
ACCACCACCT CCATTGGTGG AAAACAGTAC TTCATTCAAA AGCCACTTAC ACTTGCTCCA 
AATGTTCAGC TCCAATTTGT TAAGACCAGT GGTGGGATGA CTGTTCAAAC TCTTCCAAAA 
GTAAACTTTA CGGCTTCAAA AGGAACGCCC CCACATGGCA TATCAATTGC AAACCCACAT 
TTGGCGTCTG GTATTACTCA AATTCAGGGA TCGACAGTGC CTGGAAGTCA GATTCAAAAG 
CCCATTGTGT CCGGAAACGT TTTAAAGCTT GTATCGCCGC ATACGATGGC CGGTGGAAAA 
TTGATTATGA AGAATTCAAA CATCTTACAG ATGGGCAAGG TAACTCCGAA CGTAATGGGT 
GGAAAACCAG CTTTTGTCAT AACTAATAAA CAGGGTACGC CATTGGGAAA TCAGCAGATT 
ATTATTGTAA CCACTGGTGG CAATGTGCGA TCTGTTCCAA CTAGCACAGT TATGACAAGT 
GCTGGTGGCT CTGCATCAGG GACAAATATT GTAAGTATAG TTAACTCAAC GTCGACCACG 
CCTAGCCCGC TGCAGGCATT AAGTGGACAA AAAACTTTAA TATCCAACCA AAGTGGAGTT 
AAGATGCTGC GAAATATATC ATCAGTACAA GCATCATCTT CAATGGCATT TGGGCAAAAA 
CAAAGTGGCA CTCCCATTCA TCAAAAAACG GCATTGTATA TAGGAGGCAA AGCTGTTACC 
GTTATGAGCA CCAATACAAG TATGGCTGCA TCCGGAAATA AAGTTATGGT ACTACCAGGA 
ACAAGCTCAA ATAATTCTCC TGCTACTACA ACGGCGCTGA GTGCCAGAAA AAGTTTTGTT 
TTCAATGCCG GAGGTAGCCC TCGGACCGTC ACTTTAGCAA CTAAAAGCAT TAATGCAAAA 
TCTATACCAC AATCGCAACC AGTGACGGAA ACTAATAACC ATTCAGTCGC TACTATTAAA 
GACACAGATC CAATGGATGA TATTATAGAG CAGTTGGATG GAGCCGGAGA TCTGCTAAAG 
CTTTCTGAAA GTGAAGGCCA GCATGGATCG GAGGAGAATG AAAATAATGG TGAAAACGCA 
ACGTCTTCAT CTGCCTCTGC CCTGTTTACC GGAGGTGATA CGGCCGGGCC ATCGAGAGCC 
CAAAACCCTA TAGTAATGGA ACATCCGGTT GATATTATAG AAGATGTCTC TGGCGTGAGC 
AGCACAACTG ACGTTAACGA AACTGCAATC GTAAGTGGTG ATACAATAGA ATCGCTTAAG 
ATGTCGGAGA AGGAAAACGA TGATGTGAAA TCTATGGGTG AAAAATCAAT ATTATCCGAT 
GATTGTCATC AGCCGACGAC TTCCGAGACG GAAGCGGCCA CCATCCTAAC CACAATTAAG 
TCTGCAGAGG CTTTAGTGTT GGAAACTGCA GAAATAAGGA AAGATCATAC CGGATGTACT 
ATAGGTAGTT TGAAAGAAAA TCAAGATGAA AATAAAAAGT TTAAACAACG GCAAGAATCT 
TCACCGTCTC AAAACATTCA CCAATTCCAA AATGTGGATG GTTCACAATT AGAGGCTCTT 
GCTTCTGCTG CGTTACTGCA AGCTGCAACA TCAGACACCA CAGCATTGGC GTTGAAAGAA 
TTGATAGAGC GACCGGAAAG TGAAACAAAT ACCAGAAGTA GCAACATTGC TGAGATTCAA 
CAAAATAATG TTCAATCCAC CTTAGCGGTC GTGGTTCCAA ACACCTCCCA AAACGAGAAC 
CAAAAATGGC ACACAGTAGG TGTGTTTAAG GATCTTTCTC ACACAGTGAC TAGCTACATT 
GATTCGAATT GTATTAGTGA TTCTTTTTTT GACGGTATAG ATGTGGATAA TCTTCCAGAT 
TTCAGCAAGT TTCCACGCAC CAATTTGGAG CCTGGAACGG CTTACCGTTT TCGACTGAGT 
GCTATCAATT CTTGCGGACG AGGAGAATGG GGAGAGATAT CAAGTTTTAA AACCTGTTTA 
CCAGGCTTTC CGGGTGCCCC CTCAGCTATT AAAATTTCCA AGGATGTCAA GGAGGGCGCT 
CACTTAACAT GGGAACCACC ACCAGCTCAA AAAACTAAGG AGATAATTGA ATATTCAGTA 
TACCTTGCAG TTAAGCCTAC TGCCAAGGAT AAGGCGTTGT CCACTCCACA ATTAGCTTTT 
GTGCGGGTTT ATGTAGGTGC GGCAAATCAA TGCACAGTGC CGAATGCTTC ACTATCTAAT 
GCACATGTGG ATTGCTCAAA TAAACCAGCA ATTATATTCC GAATCGCTGC TCGCAATCAA 
AAGGGCTATG GACCTGCCAC CCAAGTTAGA TGGCTGCAGG ATCCCGCGGC AGCGAAACAG 
CACACACCCA CAGTTACGCC AAATCTAAAA CGTGGACCAG AAAAATCAAC TATTGGAAGC 
AGCAACATAG CAAACACCTT CTGTTCACCA CACAAGCGTG GACGCAACGG ATTGCATGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcf ( NM_166757.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726567.2 Drosophila melanogaster host cell factor, transcript variant F (Hcf), mRNA.

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CloneID	OFa00263
Clone ID Related Accession (Same CDS sequence)	NM_166757.3
Accession Version	NM_166757.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	host cell factor, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166757.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGGCT CAGACTTTGT GGATCCAGCC TTTTCTTCAG GAGAACGAAT ATCGGCATCT 
GATCTCAATT CGGAGCATAT TATTCAGGCC GAGAATCACA GTTTTGCTAA TCGAATTTCT 
ATGGATATGG ATGTCCCTGA TGGGCACCAG CTGGACAGCA ACTTAACCGG ATTTCGATGG 
AAGCGAGTTC TTAACCCAAC CGGACCGCAG CCCCGCCCGA GACATGGACA CCGTGCGATA 
AACATCAAAG AGCTAATGGT CGTCTTCGGC GGCGGAAACG AAGGAATTGT TGACGAGTTG 
CACGTTTATA ATACTGTTAC TAATCAGTGG TACGTCCCAG TGCTAAAAGG TGATGTACCG 
AACGGATGCG CTGCTTATGG ATTTGTTGTG GAGGGTACTC GCATGTTTGT TTTTGGGGGA 
ATGATCGAAT ACGGAAAATA TTCAAACGAG CTCTATGAGC TACAGGCAAC TAAATGGGAA 
TGGAGAAAAA TGTATCCAGA GTCACCAGAC AGTGGGTTGT CACCGTGTCC TCGCTTGGGT 
CACAGCTTTA CAATGGTTGG TGAAAAAATA TTTCTTTTCG GCGGACTAGC AAATGAATCG 
GACGACCCAA AAAATAATAT TCCAAAATAC TTAAACGACT TGTACATACT GGATACTCGA 
GGAGTTCACA GTCATAACGG AAAGTGGATA GTACCTAAAA CATATGGGGA TAGCCCGCCA 
CCACGAGAGT CGCACACAGG CATATCGTTT GCTACTAAAA GCAACGGAAA TTTAAATCTT 
TTGATTTATG GTGGAATGAG CGGCTGCCGC TTGGGAGATT TATGGTTGCT AGAAACAGAC 
TCCATGACTT GGTCGAAGCC GAAAACTTCA GGGGAGGCGC CGCTACCACG CTCGTTGCAC 
AGTTCTACAA TGATTGGCAA CAAAATGTAT GTCTTTGGTG GCTGGGTTCC CTTAGTGATC 
AATGATTCTA AATCGACAAC AGAGCGTGAA TGGAAATGTA CAAACACTCT TGCTGTCCTT 
GACCTTGAAA CAATGACATG GGAAAATGTC ACATTGGACA CTGTAGAGGA AAATGTTCCA 
CGTGCCCGTG CTGGCCACTG TGCAGTTGGT ATTCAAAGTC GTCTTTATGT GTGGTCAGGT 
CGTGATGGTT ACCGCAAAGC ATGGAACAAC CAGCCATTAA ATACAAGCTC GAAACTGTTA 
AGCAACAACA TTGTTCAACA AGGAACCCCG ACATCTGCTG AAACTTCTGG AATTAATATT 
TCAGCAAATA GATCTGGGAG TGCTTTGGGT CTTGGTGTCG AAGCCACATC TACAGTCTTA 
AAACTAGAAA AGGAATCTTT GCAACTGAGT GGATGTCAAC CGGAAACAAA TGTTCAACCA 
TCTGTAAATG ACTTGTTGCA ATCAATGTCG CAACCTTCTT CCCCAGCGTC CCGTGCTGAT 
AAAGACCCAC TTTCGTCAGG GGGTGGAACT ACCTTTAACT TATCAACATC GGTCGCTTCA 
GTGCATCCTC AAATATCTGT AATCAGTAGT ACAGCCGCAG TAACGGGCAA CGACACAGCG 
TCCCCAAGTG GCGCCATTAA TAGTATATTG CAAAAGTTTC GACCAGTTGT TACCGCTGTA 
AGAACGTCCA CAACTACTGC GGTATCTATT GCTACCAGCA CGTCTGATCC CTTGTCTGTG 
CGAGTACCAA GTACGATGTC GGCAAACGTT GTTCTCAGCT CTTCAAGTAG TACATTGCGT 
ATTGTCCCAA GCGTAACTGC ATCGCATTCA CTACGTATAG CTTCTTCTCA AGCAAGTGGC 
AACAACTGCC GGAGTAGCTC TGCAATAAAT ATATTAAAAA CAGCTCTTCC AAACGTCGCC 
GTGCAATCTC AGCCTACATC ATCGACCACC ACCTCCATTG GTGGAAAACA GTACTTCATT 
CAAAAGCCAC TTACACTTGC TCCAAATGTT CAGCTCCAAT TTGTTAAGAC CAGTGGTGGG 
ATGACTGTTC AAACTCTTCC AAAAGTAAAC TTTACGGCTT CAAAAGGAAC GCCCCCACAT 
GGCATATCAA TTGCAAACCC ACATTTGGCG TCTGGTATTA CTCAAATTCA GGGATCGACA 
GTGCCTGGAA GTCAGATTCA AAAGCCCATT GTGTCCGGAA ACGTTTTAAA GCTTGTATCG 
CCGCATACGA TGGCCGGTGG AAAATTGATT ATGAAGAATT CAAACATCTT ACAGATGGGC 
AAGGTAACTC CGAACGTAAT GGGTGGAAAA CCAGCTTTTG TCATAACTAA TAAACAGGGT 
ACGCCATTGG GAAATCAGCA GATTATTATT GTAACCACTG GTGGCAATGT GCGATCTGTT 
CCAACTAGCA CAGTTATGAC AAGTGCTGGT GGCTCTGCAT CAGGGACAAA TATTGTAAGT 
ATAGTTAACT CAACGTCGAC CACGCCTAGC CCGCTGCAGG CATTAAGTGG ACAAAAAACT 
TTAATATCCA ACCAAAGTGG AGTTAAGATG CTGCGAAATA TATCATCAGT ACAAGCATCA 
TCTTCAATGG CATTTGGGCA AAAACAAAGT GGCACTCCCA TTCATCAAAA AACGGCATTG 
TATATAGGAG GCAAAGCTGT TACCGTTATG AGCACCAATA CAAGTATGGC TGCATCCGGA 
AATAAAGTTA TGGTACTACC AGGAACAAGC TCAAATAATT CTCCTGCTAC TACAACGGCG 
CTGAGTGCCA GAAAAAGTTT TGTTTTCAAT GCCGGAGGTA GCCCTCGGAC CGTCACTTTA 
GCAACTAAAA GCATTAATGC AAAATCTATA CCACAATCGC AACCAGTGAC GGAAACTAAT 
AACCATTCAG TCGCTACTAT TAAAGACACA GATCCAATGG ATGATATTAT AGAGCAGTTG 
GATGGAGCCG GAGATCTGCT AAAGCTTTCT GAAAGTGAAG GCCAGCATGG ATCGGAGGAG 
AATGAAAATA ATGGTGAAAA CGCAACGTCT TCATCTGCCT CTGCCCTGTT TACCGGAGGT 
GATACGGCCG GGCCATCGAG AGCCCAAAAC CCTATAGTAA TGGAACATCC GGTTGATATT 
ATAGAAGATG TCTCTGGCGT GAGCAGCACA ACTGACGTTA ACGAAACTGC AATCGTAAGT 
GGTGATACAA TAGAATCGCT TAAGATGTCG GAGAAGGAAA ACGATGATGT GAAATCTATG 
GGTGAAAAAT CAATATTATC CGATGATTGT CATCAGCCGA CGACTTCCGA GACGGAAGCG 
GCCACCATCC TAACCACAAT TAAGTCTGCA GAGGCTTTAG TGTTGGAAAC TGCAGAAATA 
AGGAAAGATC ATACCGGATG TACTATAGGT AGTTTGAAAG AAAATCAAGA TGAAAATAAA 
AAGTTTAAAC AACGGCAAGA ATCTTCACCG TCTCAAAACA TTCACCAATT CCAAAATGTG 
GATGGTTCAC AATTAGAGGC TCTTGCTTCT GCTGCGTTAC TGCAAGCTGC AACATCAGAC 
ACCACAGCAT TGGCGTTGAA AGAATTGATA GAGCGACCGG AAAGTGAAAC AAATACCAGA 
AGTAGCAACA TTGCTGAGAT TCAACAAAAT AATGTTCAAT CCACCTTAGC GGTCGTGGTT 
CCAAACACCT CCCAAAACGA GAACCAAAAA TGGCACACAG TAGGTGTGTT TAAGGATCTT 
TCTCACACAG TGACTAGCTA CATTGATTCG AATTGTATTA GTGATTCTTT TTTTGACGGT 
ATAGATGTGG ATAATCTTCC AGATTTCAGC AAGTTTCCAC GCACCAATTT GGAGCCTGGA 
ACGGCTTACC GTTTTCGACT GAGTGCTATC AATTCTTGCG GACGAGGAGA ATGGGGAGAG 
ATATCAAGTT TTAAAACCTG TTTACCAGGC TTTCCGGGTG CCCCCTCAGC TATTAAAATT 
TCCAAGGATG TCAAGGAGGG CGCTCACTTA ACATGGGAAC CACCACCAGC TCAAAAAACT 
AAGGAGATAA TTGAATATTC AGTATACCTT GCAGTTAAGC CTACTGCCAA GGATAAGGCG 
TTGTCCACTC CACAATTAGC TTTTGTGCGG GTTTATGTAG GTGCGGCAAA TCAATGCACA 
GTGCCGAATG CTTCACTATC TAATGCACAT GTGGATTGCT CAAATAAACC AGCAATTATA 
TTCCGAATCG CTGCTCGCAA TCAAAAGGGC TATGGACCTG CCACCCAAGT TAGATGGCTG 
CAGGATCCCG CGGCAGCGAA ACAGCACACA CCCACAGTTA CGCCAAATCT AAAACGTGGA 
CCAGAAAAAT CAACTATTGG AAGCAGCAAC ATAGCAAACA CCTTCTGTTC ACCACACAAG 
CGTGGACGCA ACGGATTGCA TGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726567.2
CDS	452..4798
Translation	MEGSDFVDPAFSSGERISASDLNSEHIIQAENHSFANRISMDMDVPDGHQLDSNLTGFRWKRVLNPTGPQPRPRHGHRAINIKELMVVFGGGNEGIVDELHVYNTVTNQWYVPVLKGDVPNGCAAYGFVVEGTRMFVFGGMIEYGKYSNELYELQATKWEWRKMYPESPDSGLSPCPRLGHSFTMVGEKIFLFGGLANESDDPKNNIPKYLNDLYILDTRGVHSHNGKWIVPKTYGDSPPPRESHTGISFATKSNGNLNLLIYGGMSGCRLGDLWLLETDSMTWSKPKTSGEAPLPRSLHSSTMIGNKMYVFGGWVPLVINDSKSTTEREWKCTNTLAVLDLETMTWENVTLDTVEENVPRARAGHCAVGIQSRLYVWSGRDGYRKAWNNQPLNTSSKLLSNNIVQQGTPTSAETSGINISANRSGSALGLGVEATSTVLKLEKESLQLSGCQPETNVQPSVNDLLQSMSQPSSPASRADKDPLSSGGGTTFNLSTSVASVHPQISVISSTAAVTGNDTASPSGAINSILQKFRPVVTAVRTSTTTAVSIATSTSDPLSVRVPSTMSANVVLSSSSSTLRIVPSVTASHSLRIASSQASGNNCRSSSAINILKTALPNVAVQSQPTSSTTTSIGGKQYFIQKPLTLAPNVQLQFVKTSGGMTVQTLPKVNFTASKGTPPHGISIANPHLASGITQIQGSTVPGSQIQKPIVSGNVLKLVSPHTMAGGKLIMKNSNILQMGKVTPNVMGGKPAFVITNKQGTPLGNQQIIIVTTGGNVRSVPTSTVMTSAGGSASGTNIVSIVNSTSTTPSPLQALSGQKTLISNQSGVKMLRNISSVQASSSMAFGQKQSGTPIHQKTALYIGGKAVTVMSTNTSMAASGNKVMVLPGTSSNNSPATTTALSARKSFVFNAGGSPRTVTLATKSINAKSIPQSQPVTETNNHSVATIKDTDPMDDIIEQLDGAGDLLKLSESEGQHGSEENENNGENATSSSASALFTGGDTAGPSRAQNPIVMEHPVDIIEDVSGVSSTTDVNETAIVSGDTIESLKMSEKENDDVKSMGEKSILSDDCHQPTTSETEAATILTTIKSAEALVLETAEIRKDHTGCTIGSLKENQDENKKFKQRQESSPSQNIHQFQNVDGSQLEALASAALLQAATSDTTALALKELIERPESETNTRSSNIAEIQQNNVQSTLAVVVPNTSQNENQKWHTVGVFKDLSHTVTSYIDSNCISDSFFDGIDVDNLPDFSKFPRTNLEPGTAYRFRLSAINSCGRGEWGEISSFKTCLPGFPGAPSAIKISKDVKEGAHLTWEPPPAQKTKEIIEYSVYLAVKPTAKDKALSTPQLAFVRVYVGAANQCTVPNASLSNAHVDCSNKPAIIFRIAARNQKGYGPATQVRWLQDPAAAKQHTPTVTPNLKRGPEKSTIGSSNIANTFCSPHKRGRNGLHD

Target ORF information:

RefSeq Version	NM_166757.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster host cell factor, transcript variant F (Hcf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166757.3

ORF Insert Sequence:

ATGGAAGGCT CAGACTTTGT GGATCCAGCC TTTTCTTCAG GAGAACGAAT ATCGGCATCT 
GATCTCAATT CGGAGCATAT TATTCAGGCC GAGAATCACA GTTTTGCTAA TCGAATTTCT 
ATGGATATGG ATGTCCCTGA TGGGCACCAG CTGGACAGCA ACTTAACCGG ATTTCGATGG 
AAGCGAGTTC TTAACCCAAC CGGACCGCAG CCCCGCCCGA GACATGGACA CCGTGCGATA 
AACATCAAAG AGCTAATGGT CGTCTTCGGC GGCGGAAACG AAGGAATTGT TGACGAGTTG 
CACGTTTATA ATACTGTTAC TAATCAGTGG TACGTCCCAG TGCTAAAAGG TGATGTACCG 
AACGGATGCG CTGCTTATGG ATTTGTTGTG GAGGGTACTC GCATGTTTGT TTTTGGGGGA 
ATGATCGAAT ACGGAAAATA TTCAAACGAG CTCTATGAGC TACAGGCAAC TAAATGGGAA 
TGGAGAAAAA TGTATCCAGA GTCACCAGAC AGTGGGTTGT CACCGTGTCC TCGCTTGGGT 
CACAGCTTTA CAATGGTTGG TGAAAAAATA TTTCTTTTCG GCGGACTAGC AAATGAATCG 
GACGACCCAA AAAATAATAT TCCAAAATAC TTAAACGACT TGTACATACT GGATACTCGA 
GGAGTTCACA GTCATAACGG AAAGTGGATA GTACCTAAAA CATATGGGGA TAGCCCGCCA 
CCACGAGAGT CGCACACAGG CATATCGTTT GCTACTAAAA GCAACGGAAA TTTAAATCTT 
TTGATTTATG GTGGAATGAG CGGCTGCCGC TTGGGAGATT TATGGTTGCT AGAAACAGAC 
TCCATGACTT GGTCGAAGCC GAAAACTTCA GGGGAGGCGC CGCTACCACG CTCGTTGCAC 
AGTTCTACAA TGATTGGCAA CAAAATGTAT GTCTTTGGTG GCTGGGTTCC CTTAGTGATC 
AATGATTCTA AATCGACAAC AGAGCGTGAA TGGAAATGTA CAAACACTCT TGCTGTCCTT 
GACCTTGAAA CAATGACATG GGAAAATGTC ACATTGGACA CTGTAGAGGA AAATGTTCCA 
CGTGCCCGTG CTGGCCACTG TGCAGTTGGT ATTCAAAGTC GTCTTTATGT GTGGTCAGGT 
CGTGATGGTT ACCGCAAAGC ATGGAACAAC CAGCCATTAA ATACAAGCTC GAAACTGTTA 
AGCAACAACA TTGTTCAACA AGGAACCCCG ACATCTGCTG AAACTTCTGG AATTAATATT 
TCAGCAAATA GATCTGGGAG TGCTTTGGGT CTTGGTGTCG AAGCCACATC TACAGTCTTA 
AAACTAGAAA AGGAATCTTT GCAACTGAGT GGATGTCAAC CGGAAACAAA TGTTCAACCA 
TCTGTAAATG ACTTGTTGCA ATCAATGTCG CAACCTTCTT CCCCAGCGTC CCGTGCTGAT 
AAAGACCCAC TTTCGTCAGG GGGTGGAACT ACCTTTAACT TATCAACATC GGTCGCTTCA 
GTGCATCCTC AAATATCTGT AATCAGTAGT ACAGCCGCAG TAACGGGCAA CGACACAGCG 
TCCCCAAGTG GCGCCATTAA TAGTATATTG CAAAAGTTTC GACCAGTTGT TACCGCTGTA 
AGAACGTCCA CAACTACTGC GGTATCTATT GCTACCAGCA CGTCTGATCC CTTGTCTGTG 
CGAGTACCAA GTACGATGTC GGCAAACGTT GTTCTCAGCT CTTCAAGTAG TACATTGCGT 
ATTGTCCCAA GCGTAACTGC ATCGCATTCA CTACGTATAG CTTCTTCTCA AGCAAGTGGC 
AACAACTGCC GGAGTAGCTC TGCAATAAAT ATATTAAAAA CAGCTCTTCC AAACGTCGCC 
GTGCAATCTC AGCCTACATC ATCGACCACC ACCTCCATTG GTGGAAAACA GTACTTCATT 
CAAAAGCCAC TTACACTTGC TCCAAATGTT CAGCTCCAAT TTGTTAAGAC CAGTGGTGGG 
ATGACTGTTC AAACTCTTCC AAAAGTAAAC TTTACGGCTT CAAAAGGAAC GCCCCCACAT 
GGCATATCAA TTGCAAACCC ACATTTGGCG TCTGGTATTA CTCAAATTCA GGGATCGACA 
GTGCCTGGAA GTCAGATTCA AAAGCCCATT GTGTCCGGAA ACGTTTTAAA GCTTGTATCG 
CCGCATACGA TGGCCGGTGG AAAATTGATT ATGAAGAATT CAAACATCTT ACAGATGGGC 
AAGGTAACTC CGAACGTAAT GGGTGGAAAA CCAGCTTTTG TCATAACTAA TAAACAGGGT 
ACGCCATTGG GAAATCAGCA GATTATTATT GTAACCACTG GTGGCAATGT GCGATCTGTT 
CCAACTAGCA CAGTTATGAC AAGTGCTGGT GGCTCTGCAT CAGGGACAAA TATTGTAAGT 
ATAGTTAACT CAACGTCGAC CACGCCTAGC CCGCTGCAGG CATTAAGTGG ACAAAAAACT 
TTAATATCCA ACCAAAGTGG AGTTAAGATG CTGCGAAATA TATCATCAGT ACAAGCATCA 
TCTTCAATGG CATTTGGGCA AAAACAAAGT GGCACTCCCA TTCATCAAAA AACGGCATTG 
TATATAGGAG GCAAAGCTGT TACCGTTATG AGCACCAATA CAAGTATGGC TGCATCCGGA 
AATAAAGTTA TGGTACTACC AGGAACAAGC TCAAATAATT CTCCTGCTAC TACAACGGCG 
CTGAGTGCCA GAAAAAGTTT TGTTTTCAAT GCCGGAGGTA GCCCTCGGAC CGTCACTTTA 
GCAACTAAAA GCATTAATGC AAAATCTATA CCACAATCGC AACCAGTGAC GGAAACTAAT 
AACCATTCAG TCGCTACTAT TAAAGACACA GATCCAATGG ATGATATTAT AGAGCAGTTG 
GATGGAGCCG GAGATCTGCT AAAGCTTTCT GAAAGTGAAG GCCAGCATGG ATCGGAGGAG 
AATGAAAATA ATGGTGAAAA CGCAACGTCT TCATCTGCCT CTGCCCTGTT TACCGGAGGT 
GATACGGCCG GGCCATCGAG AGCCCAAAAC CCTATAGTAA TGGAACATCC GGTTGATATT 
ATAGAAGATG TCTCTGGCGT GAGCAGCACA ACTGACGTTA ACGAAACTGC AATCGTAAGT 
GGTGATACAA TAGAATCGCT TAAGATGTCG GAGAAGGAAA ACGATGATGT GAAATCTATG 
GGTGAAAAAT CAATATTATC CGATGATTGT CATCAGCCGA CGACTTCCGA GACGGAAGCG 
GCCACCATCC TAACCACAAT TAAGTCTGCA GAGGCTTTAG TGTTGGAAAC TGCAGAAATA 
AGGAAAGATC ATACCGGATG TACTATAGGT AGTTTGAAAG AAAATCAAGA TGAAAATAAA 
AAGTTTAAAC AACGGCAAGA ATCTTCACCG TCTCAAAACA TTCACCAATT CCAAAATGTG 
GATGGTTCAC AATTAGAGGC TCTTGCTTCT GCTGCGTTAC TGCAAGCTGC AACATCAGAC 
ACCACAGCAT TGGCGTTGAA AGAATTGATA GAGCGACCGG AAAGTGAAAC AAATACCAGA 
AGTAGCAACA TTGCTGAGAT TCAACAAAAT AATGTTCAAT CCACCTTAGC GGTCGTGGTT 
CCAAACACCT CCCAAAACGA GAACCAAAAA TGGCACACAG TAGGTGTGTT TAAGGATCTT 
TCTCACACAG TGACTAGCTA CATTGATTCG AATTGTATTA GTGATTCTTT TTTTGACGGT 
ATAGATGTGG ATAATCTTCC AGATTTCAGC AAGTTTCCAC GCACCAATTT GGAGCCTGGA 
ACGGCTTACC GTTTTCGACT GAGTGCTATC AATTCTTGCG GACGAGGAGA ATGGGGAGAG 
ATATCAAGTT TTAAAACCTG TTTACCAGGC TTTCCGGGTG CCCCCTCAGC TATTAAAATT 
TCCAAGGATG TCAAGGAGGG CGCTCACTTA ACATGGGAAC CACCACCAGC TCAAAAAACT 
AAGGAGATAA TTGAATATTC AGTATACCTT GCAGTTAAGC CTACTGCCAA GGATAAGGCG 
TTGTCCACTC CACAATTAGC TTTTGTGCGG GTTTATGTAG GTGCGGCAAA TCAATGCACA 
GTGCCGAATG CTTCACTATC TAATGCACAT GTGGATTGCT CAAATAAACC AGCAATTATA 
TTCCGAATCG CTGCTCGCAA TCAAAAGGGC TATGGACCTG CCACCCAAGT TAGATGGCTG 
CAGGATCCCG CGGCAGCGAA ACAGCACACA CCCACAGTTA CGCCAAATCT AAAACGTGGA 
CCAGAAAAAT CAACTATTGG AAGCAGCAAC ATAGCAAACA CCTTCTGTTC ACCACACAAG 
CGTGGACGCA ACGGATTGCA TGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hcf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

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