Rad23 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rad23
Entrez Gene ID	43785
Full Name	CG1836 gene product from transcript CG1836-RC
Synonyms	CG1836,DHR23,Dmel\CG1836,RAD23,rad23
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102B3-102B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_143661.3	NP_651918.2	Rad23, isoform A
NM_166752.2	NP_726561.1	Rad23, isoform B
NM_001272123.1	NP_001259052.1	Rad23, isoform C

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rhp23	NP_596231.1
Pan troglodytes (chimpanzee)	RAD23B	NP_001231638.1
Canis lupus familiaris (dog)	RAD23B	XP_538778.1
Rattus norvegicus (Norway rat)	Rad23b	NP_001020446.1
Danio rerio (zebrafish)	rad23b	NP_956858.1
Drosophila melanogaster (fruit fly)	Rad23	NP_001259052.1
Saccharomyces cerevisiae (baker's yeast)	RAD23	NP_010877.3
Arabidopsis thaliana (thale cress)	RAD23C	NP_186903.1
Arabidopsis thaliana (thale cress)	RAD23D	NP_198663.1
Bos taurus (cattle)	RAD23B	NP_001039775.1
Gallus gallus (chicken)	RAD23B	XP_429175.3
Neurospora crassa	NCU07542	XP_959055.2
Caenorhabditis elegans (roundworm)	rad-23	NP_496488.2
Kluyveromyces lactis	KLLA0E22133g	XP_454953.1
Eremothecium gossypii	AGOS_AFR358W	NP_985905.1
Oryza sativa (rice)	Os06g0264300	NP_001057334.1
Xenopus tropicalis (tropical clawed frog)	rad23b	NP_001025578.1
Homo sapiens (human)	RAD23B	NP_002865.1
Mus musculus (house mouse)	Rad23b	NP_033037.2
Magnaporthe oryzae (rice blast fungus)	MGG_01656	XP_003714642.1
Oryza sativa (rice)	Os09g0407200	NP_001063134.1
Oryza sativa (rice)	Os08g0430200	NP_001061854.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Additional studies established that the increased toxicity upon mutating UbS2 stems from disrupting the autoprotective role that pathogenic ataxin-3 has against itself, which depends on the co-chaperone, DnaJ-1. Our data reveal a previously unrecognized balance between pathogenic and potentially therapeutic properties of the ataxin-3-Rad23 interaction; they highlight this interaction as critical for the toxicity of the S
Title: Interaction of the polyglutamine protein ataxin-3 with Rad23 regulates toxicity in Drosophila models of Spinocerebellar Ataxia Type 3.

Through DnaJ-1, our data connect ataxin-3 and Rad23 to protective processes involved with protein folding rather than increased turnover of toxic polyQ species.
Title: The deubiquitinase ataxin-3 requires Rad23 and DnaJ-1 for its neuroprotective role in Drosophila melanogaster.

The following Rad23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rad23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00152	NM_001272123.1 Latest version!	Drosophila melanogaster Rad23, transcript variant C (Rad23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00152	NM_143661.3 Latest version!	Drosophila melanogaster Rad23, transcript variant A (Rad23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00256	NM_166752.2 Latest version!	Drosophila melanogaster Rad23, transcript variant B (Rad23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rad23 ( NM_143661.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651918.2 Drosophila melanogaster Rad23, transcript variant A (Rad23), mRNA.

Price & Availability↑

CloneID	OFa00152
Clone ID Related Accession (Same CDS sequence)	NM_143661.3 , NM_001272123.1
Accession Version	NM_143661.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rad23, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_143661.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTATTA CAATTAAAAA TCTTCAACAG CAAACTTTTA CTATTGAGTT TGCCCCGGAA 
AAAACGGTTT TGGAACTGAA GAAGAAAATA TTCGAGGAGC GCGGTCCAGA GTACGTCGCC 
GAAAAACAAA AACTGATCTA CGCTGGCGTC ATATTGACGG ATGACCGCAC CGTTGGTTCA 
TACAACGTTG ATGAAAAAAA GTTCATTGTG GTAATGTTGA CACGCGATTC GTCTAGTTCA 
AATCGTAATC AACTTAGTGT AAAAGAAAGT AATAAATTGA CTAGCACCGA CGATTCAAAG 
CAATCTATGC CTTGCGAAGA AGCCAACCAT ACCAATTCGC CTAGTTCCAC AAATACAGAA 
GATTCAGTTT TATCACGTGA AACCAGACCT TTATCTAGTG ACGAATTGAT TGGCGAGTTG 
GCCCAGGCTT CCTTACAATC GCGCGCTGAA TCTAATTTGC TTATGGGTGA CGAATACAAC 
CAAACAGTCC TATCAATGGT GGAAATGGGT TACCCAAGAG AGCAGGTTGA ACGTGCGATG 
GCTGCTAGTT ATAACAACCC GGAAAGAGCC GTTGAATATC TCATTAATGG CATACCTGCA 
GAGGAAGGTA CTTTTTACAA TAGGCTGAAT GAATCAACGA ATCCTAGTCT AATCCCCTCC 
GGACCGCAAC CTGCGTCGGC AACCTCTGCC GAGCGTTCAA CAGAATCAAA TTCAGACCCC 
TTTGAATTTT TACGTAGCCA GCCACAGTTT CTTCAAATGC GATCTTTAAT TTATCAAAAC 
CCTCATCTTT TGCATGCAGT ATTGCAGCAG ATTGGTCAGA CAAACCCAGC CCTTTTACAA 
CTTATTTCGG AGAACCAAGA TGCGTTTCTC AATATGCTTA ATCAACCGAT TGACCGCGAA 
TCGGAGTCAG GCGCCACCGT TCCTCCCGTT TCGAATGCGC GGATTCCTTC AACTTTGGAC 
AATGTTGACC TCTTTTCTCC AGATTTAGAA GTAGCTACTT CAGCACAAAG ATCAGCCGCG 
GGTACAAGTG CAGCACATCA AAGCGGTAGC GCAGCGGATA ACGAAGACTT GGAACAACCT 
TTAGGAGTAT CAACCATTCG TTTAAATCGC CAAGATAAGG ACGCAATAGA ACGGCTAAAA 
GCTCTTGGAT TCCCAGAGGC CCTTGTACTG CAAGCGTACT TCGCTTGTGA AAAGAACGAG 
GAACAAGCAG CTAATTTTTT GCTATCGTCT AGCTTCGATG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651918.2
CDS	132..1376
Translation	MIITIKNLQQQTFTIEFAPEKTVLELKKKIFEERGPEYVAEKQKLIYAGVILTDDRTVGSYNVDEKKFIVVMLTRDSSSSNRNQLSVKESNKLTSTDDSKQSMPCEEANHTNSPSSTNTEDSVLSRETRPLSSDELIGELAQASLQSRAESNLLMGDEYNQTVLSMVEMGYPREQVERAMAASYNNPERAVEYLINGIPAEEGTFYNRLNESTNPSLIPSGPQPASATSAERSTESNSDPFEFLRSQPQFLQMRSLIYQNPHLLHAVLQQIGQTNPALLQLISENQDAFLNMLNQPIDRESESGATVPPVSNARIPSTLDNVDLFSPDLEVATSAQRSAAGTSAAHQSGSAADNEDLEQPLGVSTIRLNRQDKDAIERLKALGFPEALVLQAYFACEKNEEQAANFLLSSSFDD

Target ORF information:

RefSeq Version	NM_143661.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rad23, transcript variant A (Rad23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143661.3

ORF Insert Sequence:

ATGATTATTA CAATTAAAAA TCTTCAACAG CAAACTTTTA CTATTGAGTT TGCCCCGGAA 
AAAACGGTTT TGGAACTGAA GAAGAAAATA TTCGAGGAGC GCGGTCCAGA GTACGTCGCC 
GAAAAACAAA AACTGATCTA CGCTGGCGTC ATATTGACGG ATGACCGCAC CGTTGGTTCA 
TACAACGTTG ATGAAAAAAA GTTCATTGTG GTAATGTTGA CACGCGATTC GTCTAGTTCA 
AATCGTAATC AACTTAGTGT AAAAGAAAGT AATAAATTGA CTAGCACCGA CGATTCAAAG 
CAATCTATGC CTTGCGAAGA AGCCAACCAT ACCAATTCGC CTAGTTCCAC AAATACAGAA 
GATTCAGTTT TATCACGTGA AACCAGACCT TTATCTAGTG ACGAATTGAT TGGCGAGTTG 
GCCCAGGCTT CCTTACAATC GCGCGCTGAA TCTAATTTGC TTATGGGTGA CGAATACAAC 
CAAACAGTCC TATCAATGGT GGAAATGGGT TACCCAAGAG AGCAGGTTGA ACGTGCGATG 
GCTGCTAGTT ATAACAACCC GGAAAGAGCC GTTGAATATC TCATTAATGG CATACCTGCA 
GAGGAAGGTA CTTTTTACAA TAGGCTGAAT GAATCAACGA ATCCTAGTCT AATCCCCTCC 
GGACCGCAAC CTGCGTCGGC AACCTCTGCC GAGCGTTCAA CAGAATCAAA TTCAGACCCC 
TTTGAATTTT TACGTAGCCA GCCACAGTTT CTTCAAATGC GATCTTTAAT TTATCAAAAC 
CCTCATCTTT TGCATGCAGT ATTGCAGCAG ATTGGTCAGA CAAACCCAGC CCTTTTACAA 
CTTATTTCGG AGAACCAAGA TGCGTTTCTC AATATGCTTA ATCAACCGAT TGACCGCGAA 
TCGGAGTCAG GCGCCACCGT TCCTCCCGTT TCGAATGCGC GGATTCCTTC AACTTTGGAC 
AATGTTGACC TCTTTTCTCC AGATTTAGAA GTAGCTACTT CAGCACAAAG ATCAGCCGCG 
GGTACAAGTG CAGCACATCA AAGCGGTAGC GCAGCGGATA ACGAAGACTT GGAACAACCT 
TTAGGAGTAT CAACCATTCG TTTAAATCGC CAAGATAAGG ACGCAATAGA ACGGCTAAAA 
GCTCTTGGAT TCCCAGAGGC CCTTGTACTG CAAGCGTACT TCGCTTGTGA AAAGAACGAG 
GAACAAGCAG CTAATTTTTT GCTATCGTCT AGCTTCGATG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad23 ( NM_001272123.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259052.1 Drosophila melanogaster Rad23, transcript variant C (Rad23), mRNA.

Price & Availability↑

CloneID	OFa00152
Clone ID Related Accession (Same CDS sequence)	NM_143661.3 , NM_001272123.1
Accession Version	NM_001272123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rad23, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTATTA CAATTAAAAA TCTTCAACAG CAAACTTTTA CTATTGAGTT TGCCCCGGAA 
AAAACGGTTT TGGAACTGAA GAAGAAAATA TTCGAGGAGC GCGGTCCAGA GTACGTCGCC 
GAAAAACAAA AACTGATCTA CGCTGGCGTC ATATTGACGG ATGACCGCAC CGTTGGTTCA 
TACAACGTTG ATGAAAAAAA GTTCATTGTG GTAATGTTGA CACGCGATTC GTCTAGTTCA 
AATCGTAATC AACTTAGTGT AAAAGAAAGT AATAAATTGA CTAGCACCGA CGATTCAAAG 
CAATCTATGC CTTGCGAAGA AGCCAACCAT ACCAATTCGC CTAGTTCCAC AAATACAGAA 
GATTCAGTTT TATCACGTGA AACCAGACCT TTATCTAGTG ACGAATTGAT TGGCGAGTTG 
GCCCAGGCTT CCTTACAATC GCGCGCTGAA TCTAATTTGC TTATGGGTGA CGAATACAAC 
CAAACAGTCC TATCAATGGT GGAAATGGGT TACCCAAGAG AGCAGGTTGA ACGTGCGATG 
GCTGCTAGTT ATAACAACCC GGAAAGAGCC GTTGAATATC TCATTAATGG CATACCTGCA 
GAGGAAGGTA CTTTTTACAA TAGGCTGAAT GAATCAACGA ATCCTAGTCT AATCCCCTCC 
GGACCGCAAC CTGCGTCGGC AACCTCTGCC GAGCGTTCAA CAGAATCAAA TTCAGACCCC 
TTTGAATTTT TACGTAGCCA GCCACAGTTT CTTCAAATGC GATCTTTAAT TTATCAAAAC 
CCTCATCTTT TGCATGCAGT ATTGCAGCAG ATTGGTCAGA CAAACCCAGC CCTTTTACAA 
CTTATTTCGG AGAACCAAGA TGCGTTTCTC AATATGCTTA ATCAACCGAT TGACCGCGAA 
TCGGAGTCAG GCGCCACCGT TCCTCCCGTT TCGAATGCGC GGATTCCTTC AACTTTGGAC 
AATGTTGACC TCTTTTCTCC AGATTTAGAA GTAGCTACTT CAGCACAAAG ATCAGCCGCG 
GGTACAAGTG CAGCACATCA AAGCGGTAGC GCAGCGGATA ACGAAGACTT GGAACAACCT 
TTAGGAGTAT CAACCATTCG TTTAAATCGC CAAGATAAGG ACGCAATAGA ACGGCTAAAA 
GCTCTTGGAT TCCCAGAGGC CCTTGTACTG CAAGCGTACT TCGCTTGTGA AAAGAACGAG 
GAACAAGCAG CTAATTTTTT GCTATCGTCT AGCTTCGATG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259052.1
CDS	132..1376
Translation	MIITIKNLQQQTFTIEFAPEKTVLELKKKIFEERGPEYVAEKQKLIYAGVILTDDRTVGSYNVDEKKFIVVMLTRDSSSSNRNQLSVKESNKLTSTDDSKQSMPCEEANHTNSPSSTNTEDSVLSRETRPLSSDELIGELAQASLQSRAESNLLMGDEYNQTVLSMVEMGYPREQVERAMAASYNNPERAVEYLINGIPAEEGTFYNRLNESTNPSLIPSGPQPASATSAERSTESNSDPFEFLRSQPQFLQMRSLIYQNPHLLHAVLQQIGQTNPALLQLISENQDAFLNMLNQPIDRESESGATVPPVSNARIPSTLDNVDLFSPDLEVATSAQRSAAGTSAAHQSGSAADNEDLEQPLGVSTIRLNRQDKDAIERLKALGFPEALVLQAYFACEKNEEQAANFLLSSSFDD

Target ORF information:

RefSeq Version	NM_001272123.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rad23, transcript variant C (Rad23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272123.1

ORF Insert Sequence:

ATGATTATTA CAATTAAAAA TCTTCAACAG CAAACTTTTA CTATTGAGTT TGCCCCGGAA 
AAAACGGTTT TGGAACTGAA GAAGAAAATA TTCGAGGAGC GCGGTCCAGA GTACGTCGCC 
GAAAAACAAA AACTGATCTA CGCTGGCGTC ATATTGACGG ATGACCGCAC CGTTGGTTCA 
TACAACGTTG ATGAAAAAAA GTTCATTGTG GTAATGTTGA CACGCGATTC GTCTAGTTCA 
AATCGTAATC AACTTAGTGT AAAAGAAAGT AATAAATTGA CTAGCACCGA CGATTCAAAG 
CAATCTATGC CTTGCGAAGA AGCCAACCAT ACCAATTCGC CTAGTTCCAC AAATACAGAA 
GATTCAGTTT TATCACGTGA AACCAGACCT TTATCTAGTG ACGAATTGAT TGGCGAGTTG 
GCCCAGGCTT CCTTACAATC GCGCGCTGAA TCTAATTTGC TTATGGGTGA CGAATACAAC 
CAAACAGTCC TATCAATGGT GGAAATGGGT TACCCAAGAG AGCAGGTTGA ACGTGCGATG 
GCTGCTAGTT ATAACAACCC GGAAAGAGCC GTTGAATATC TCATTAATGG CATACCTGCA 
GAGGAAGGTA CTTTTTACAA TAGGCTGAAT GAATCAACGA ATCCTAGTCT AATCCCCTCC 
GGACCGCAAC CTGCGTCGGC AACCTCTGCC GAGCGTTCAA CAGAATCAAA TTCAGACCCC 
TTTGAATTTT TACGTAGCCA GCCACAGTTT CTTCAAATGC GATCTTTAAT TTATCAAAAC 
CCTCATCTTT TGCATGCAGT ATTGCAGCAG ATTGGTCAGA CAAACCCAGC CCTTTTACAA 
CTTATTTCGG AGAACCAAGA TGCGTTTCTC AATATGCTTA ATCAACCGAT TGACCGCGAA 
TCGGAGTCAG GCGCCACCGT TCCTCCCGTT TCGAATGCGC GGATTCCTTC AACTTTGGAC 
AATGTTGACC TCTTTTCTCC AGATTTAGAA GTAGCTACTT CAGCACAAAG ATCAGCCGCG 
GGTACAAGTG CAGCACATCA AAGCGGTAGC GCAGCGGATA ACGAAGACTT GGAACAACCT 
TTAGGAGTAT CAACCATTCG TTTAAATCGC CAAGATAAGG ACGCAATAGA ACGGCTAAAA 
GCTCTTGGAT TCCCAGAGGC CCTTGTACTG CAAGCGTACT TCGCTTGTGA AAAGAACGAG 
GAACAAGCAG CTAATTTTTT GCTATCGTCT AGCTTCGATG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad23 ( NM_166752.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726561.1 Drosophila melanogaster Rad23, transcript variant B (Rad23), mRNA.

Price & Availability↑

CloneID	OFa00256
Clone ID Related Accession (Same CDS sequence)	NM_166752.2
Accession Version	NM_166752.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rad23, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166752.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACAC GCGATTCGTC TAGTTCAAAT CGTAATCAAC TTAGTGTAAA AGAAAGTAAT 
AAATTGACTA GCACCGACGA TTCAAAGCAA TCTATGCCTT GCGAAGAAGC CAACCATACC 
AATTCGCCTA GTTCCACAAA TACAGAAGAT TCAGTTTTAT CACGTGAAAC CAGACCTTTA 
TCTAGTGACG AATTGATTGG CGAGTTGGCC CAGGCTTCCT TACAATCGCG CGCTGAATCT 
AATTTGCTTA TGGGTGACGA ATACAACCAA ACAGTCCTAT CAATGGTGGA AATGGGTTAC 
CCAAGAGAGC AGGTTGAACG TGCGATGGCT GCTAGTTATA ACAACCCGGA AAGAGCCGTT 
GAATATCTCA TTAATGGCAT ACCTGCAGAG GAAGGTACTT TTTACAATAG GCTGAATGAA 
TCAACGAATC CTAGTCTAAT CCCCTCCGGA CCGCAACCTG CGTCGGCAAC CTCTGCCGAG 
CGTTCAACAG AATCAAATTC AGACCCCTTT GAATTTTTAC GTAGCCAGCC ACAGTTTCTT 
CAAATGCGAT CTTTAATTTA TCAAAACCCT CATCTTTTGC ATGCAGTATT GCAGCAGATT 
GGTCAGACAA ACCCAGCCCT TTTACAACTT ATTTCGGAGA ACCAAGATGC GTTTCTCAAT 
ATGCTTAATC AACCGATTGA CCGCGAATCG GAGTCAGGCG CCACCGTTCC TCCCGTTTCG 
AATGCGCGGA TTCCTTCAAC TTTGGACAAT GTTGACCTCT TTTCTCCAGA TTTAGAAGTA 
GCTACTTCAG CACAAAGATC AGCCGCGGGT ACAAGTGCAG CACATCAAAG CGGTAGCGCA 
GCGGATAACG AAGACTTGGA ACAACCTTTA GGAGTATCAA CCATTCGTTT AAATCGCCAA 
GATAAGGACG CAATAGAACG GCTAAAAGCT CTTGGATTCC CAGAGGCCCT TGTACTGCAA 
GCGTACTTCG CTTGTGAAAA GAACGAGGAA CAAGCAGCTA ATTTTTTGCT ATCGTCTAGC 
TTCGATGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726561.1
CDS	363..1394
Translation	MLTRDSSSSNRNQLSVKESNKLTSTDDSKQSMPCEEANHTNSPSSTNTEDSVLSRETRPLSSDELIGELAQASLQSRAESNLLMGDEYNQTVLSMVEMGYPREQVERAMAASYNNPERAVEYLINGIPAEEGTFYNRLNESTNPSLIPSGPQPASATSAERSTESNSDPFEFLRSQPQFLQMRSLIYQNPHLLHAVLQQIGQTNPALLQLISENQDAFLNMLNQPIDRESESGATVPPVSNARIPSTLDNVDLFSPDLEVATSAQRSAAGTSAAHQSGSAADNEDLEQPLGVSTIRLNRQDKDAIERLKALGFPEALVLQAYFACEKNEEQAANFLLSSSFDD

Target ORF information:

RefSeq Version	NM_166752.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rad23, transcript variant B (Rad23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166752.2

ORF Insert Sequence:

ATGTTGACAC GCGATTCGTC TAGTTCAAAT CGTAATCAAC TTAGTGTAAA AGAAAGTAAT 
AAATTGACTA GCACCGACGA TTCAAAGCAA TCTATGCCTT GCGAAGAAGC CAACCATACC 
AATTCGCCTA GTTCCACAAA TACAGAAGAT TCAGTTTTAT CACGTGAAAC CAGACCTTTA 
TCTAGTGACG AATTGATTGG CGAGTTGGCC CAGGCTTCCT TACAATCGCG CGCTGAATCT 
AATTTGCTTA TGGGTGACGA ATACAACCAA ACAGTCCTAT CAATGGTGGA AATGGGTTAC 
CCAAGAGAGC AGGTTGAACG TGCGATGGCT GCTAGTTATA ACAACCCGGA AAGAGCCGTT 
GAATATCTCA TTAATGGCAT ACCTGCAGAG GAAGGTACTT TTTACAATAG GCTGAATGAA 
TCAACGAATC CTAGTCTAAT CCCCTCCGGA CCGCAACCTG CGTCGGCAAC CTCTGCCGAG 
CGTTCAACAG AATCAAATTC AGACCCCTTT GAATTTTTAC GTAGCCAGCC ACAGTTTCTT 
CAAATGCGAT CTTTAATTTA TCAAAACCCT CATCTTTTGC ATGCAGTATT GCAGCAGATT 
GGTCAGACAA ACCCAGCCCT TTTACAACTT ATTTCGGAGA ACCAAGATGC GTTTCTCAAT 
ATGCTTAATC AACCGATTGA CCGCGAATCG GAGTCAGGCG CCACCGTTCC TCCCGTTTCG 
AATGCGCGGA TTCCTTCAAC TTTGGACAAT GTTGACCTCT TTTCTCCAGA TTTAGAAGTA 
GCTACTTCAG CACAAAGATC AGCCGCGGGT ACAAGTGCAG CACATCAAAG CGGTAGCGCA 
GCGGATAACG AAGACTTGGA ACAACCTTTA GGAGTATCAA CCATTCGTTT AAATCGCCAA 
GATAAGGACG CAATAGAACG GCTAAAAGCT CTTGGATTCC CAGAGGCCCT TGTACTGCAA 
GCGTACTTCG CTTGTGAAAA GAACGAGGAA CAAGCAGCTA ATTTTTTGCT ATCGTCTAGC 
TTCGATGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
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Rad23 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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