ci cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ci
Entrez Gene ID	43767
Full Name	cubitus interruptus
Synonyms	CG2125,CI,CID,Ce,Ci,Ci/GLI,Ci/Gli,Ci155,CiD,Ci[D],Dmel\CG2125,Gli,Siah,ci-D,ci155,ciD,ci[D],l(4)102ABc,l(4)13,l(4)17
General protein information	Preferred Names cubitus interruptus Names CG2125 gene product from transcript CG2125-RB CG2125-PA CG2125-PB CG2125-PC cell ci-PA ci-PB ci-PC cubitis interruptus cubitus interuptus cubitus-interruptus cubitus-interruptus-Dominant
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102A1-102A3\|4-0.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079878.5	NP_524617.3	cubitus interruptus, isoform A
NM_001258473.1	NP_001245402.1	cubitus interruptus, isoform B
NM_001258472.2	NP_001245401.1	cubitus interruptus, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004637	XP_315176.5
Drosophila melanogaster (fruit fly)	ci	NP_524617.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study indicated that Sqh is crucial to regulate the stability of the transcription factor Cubitus interruptus (Ci) in Hedgehog (Hh) pathway.
Title: Sqh is involved in the regulation of Ci stability in Hh signaling pathway.

Ci influences the Hippo signaling via interaction with Hpo and Yki.
Title: Ci antagonizes Hippo signaling in the somatic cells of the ovary to drive germline stem cell differentiation.

findings reveal a conserved mechanism by which Ci/Gli is stabilized by a deubiquitination enzyme and identify Usp7/HUASP as a critical regulator of Hh signaling
Title: Deubiquitination of Ci/Gli by Usp7/HAUSP Regulates Hedgehog Signaling.

Multisite interaction with Sufu regulates Ci/Gli activity through distinct mechanisms in Hh signal transduction.
Title: Multisite interaction with Sufu regulates Ci/Gli activity through distinct mechanisms in Hh signal transduction.

A Comparison of Ci/Gli Activity as Regulated by Sufu in Drosophila and Mammalian Hedgehog Response
Title: A Comparison of Ci/Gli Activity as Regulated by Sufu in Drosophila and Mammalian Hedgehog Response.

The following ci gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ci cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00131	NM_001258472.2 Latest version!	Drosophila melanogaster cubitus interruptus (ci), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa00130	NM_001258473.1 Latest version!	Drosophila melanogaster cubitus interruptus (ci), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa00247	NM_079878.5 Latest version!	Drosophila melanogaster cubitus interruptus (ci), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ci ( NM_001258472.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245401.1 Drosophila melanogaster cubitus interruptus (ci), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00131
Clone ID Related Accession (Same CDS sequence)	NM_001258472.2
Accession Version	NM_001258472.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cubitus interruptus, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001258472.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCCT ACGCGTTACC TACATATTTT CCTCTTGCGT ATTCTGAATT GCAGTTTTTA 
GCGTCCAGGA GAGCAGCTGC CGTCGCTGCA GCGGCTACTG TTTTACCAGG ATCACCATGC 
ATAAACCAAC ATCACCCAAC TGACGTTTCA AGCTCGGTAA CAGTGCCATC AATTATTCCA 
ACGGGTGGAA CATCAGATTC AATTAAAACT TCAATACAAC CACAAATATG CAATGAAAAC 
ACCCTTCTTG GAAATGCTGG CCACCAGCAC AATCATCAGC CTCAACATGT TCACAATATA 
AATGTTACTG GACAGCCACA TGACTTTCAC CCGGCCTATA GAATTCCCGG ATACATGGAA 
CAGTTGTATT CTCTTCAACG AACTAATTCA GCTTCATCTT TTCACGATCC CTATGTCAAT 
TGTGCATCAG CATTCCATCT TGCCGGACTT GGTTTGGGAT CAGCTGATTT TTTGGGCAGC 
CGAGGTTTGA GCTCTTTGGG TGAACTGCAT AATGCTGCTG TGGCAGCAGC AGCAGCCGGA 
TCTCTCGCAA GTACTGATTT TCACTTCAGT GTTGATGGAA ACAGGCGGTT GGGTAGCCCT 
CGACCACCAG GAGGAAGTAT ACGTGCCAGC ATAAGCCGCA AACGAGCACT TTCGTCATCG 
CCTTATTCCG ATTCATTTGA TATTAATTCA ATGATTCGAT TTTCGCCAAA CTCTTTAGCC 
ACAATTATGA ACGGTTCTCG AGGAAGCAGC GCGGCTAGTG GTTCTTACGG TCATATTTCT 
GCTACCGCCC TTAATCCCAT GTCTCATGTT CATTCAACCC GTCTGCAACA GATACAAGCT 
CATTTATTGC GTGCAAGTGC AGGTCTGTTA AATCCGATGA CACCACAACA AGTTGCTGCA 
TCTGGATTTT CTATAGGGCA TATGCCAACC TCTGCTTCTT TGAGAGTGAA TGACGTACAT 
CCTAATCTAA GCGATTCACA TATCCAGATA ACAACATCCC CAACTGTTAC AAAGGATGTA 
AGCCAGGTCC CAGCGGCAGC ATTCTCGTTG AAGAACTTAG ATGATGCAAG AGAGAAAAAG 
GGACCCTTTA AAGATGTTGT CCCCGAGCAG CCTTCATCGA CATCTGGCGG CGTAGCACAA 
GTAGAAGCCG ACAGTGCGTC TTCACAGTTG TCTGACCGTT GTTACAACAA CGTGGTAAAC 
AATATTACAG GCATTCCTGG TGACGTTAAA GTAAACAGCC GCTTGGATGA GTATATTAAT 
TGCGGATCAA TTTCGATTCC GTCAAATGAA TATGATTGTG CTAATGCTGA TACTACGGAT 
ATCAAGGATG AACCCGGAGA TTTCATTGAA ACAAATTGTC ATTGGCGTAG CTGTCGCATT 
GAGTTCATTA CGCAAGATGA ACTTGTTAAA CATATAAATA ATGATCATAT TCAAACAAAC 
AAGAAAGCTT TTGTCTGTCG ATGGGAAGAT TGTACACGCG GTGAAAAACC ATTTAAAGCG 
CAGTATATGC TTGTTGTGCA TATGCGGCGT CATACCGGTG AAAAGCCACA CAAATGCACG 
GAGAAAAACC ATACACTTGC GAGTATCCGG GATGTAGCAA AGCCTTTAGT AATGCTAGTG 
ATCGCGCAAA GCATCAAAAT CGAACACACA GTAATGAGAA ACCGTACATT TGTAAAGCAC 
CTGGATGCAC AAAACGTTAC ACCGACCCGA GCTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245401.1
CDS	464..2182
Translation	MDAYALPTYFPLAYSELQFLASRRAAAVAAAATVLPGSPCINQHHPTDVSSSVTVPSIIPTGGTSDSIKTSIQPQICNENTLLGNAGHQHNHQPQHVHNINVTGQPHDFHPAYRIPGYMEQLYSLQRTNSASSFHDPYVNCASAFHLAGLGLGSADFLGSRGLSSLGELHNAAVAAAAAGSLASTDFHFSVDGNRRLGSPRPPGGSIRASISRKRALSSSPYSDSFDINSMIRFSPNSLATIMNGSRGSSAASGSYGHISATALNPMSHVHSTRLQQIQAHLLRASAGLLNPMTPQQVAASGFSIGHMPTSASLRVNDVHPNLSDSHIQITTSPTVTKDVSQVPAAAFSLKNLDDAREKKGPFKDVVPEQPSSTSGGVAQVEADSASSQLSDRCYNNVVNNITGIPGDVKVNSRLDEYINCGSISIPSNEYDCANADTTDIKDEPGDFIETNCHWRSCRIEFITQDELVKHINNDHIQTNKKAFVCRWEDCTRGEKPFKAQYMLVVHMRRHTGEKPHKCTEKNHTLASIRDVAKPLVMLVIAQSIKIEHTVMRNRTFVKHLDAQNVTPTRAL

Target ORF information:

RefSeq Version	NM_001258472.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cubitus interruptus (ci), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258472.2

ORF Insert Sequence:

ATGGACGCCT ACGCGTTACC TACATATTTT CCTCTTGCGT ATTCTGAATT GCAGTTTTTA 
GCGTCCAGGA GAGCAGCTGC CGTCGCTGCA GCGGCTACTG TTTTACCAGG ATCACCATGC 
ATAAACCAAC ATCACCCAAC TGACGTTTCA AGCTCGGTAA CAGTGCCATC AATTATTCCA 
ACGGGTGGAA CATCAGATTC AATTAAAACT TCAATACAAC CACAAATATG CAATGAAAAC 
ACCCTTCTTG GAAATGCTGG CCACCAGCAC AATCATCAGC CTCAACATGT TCACAATATA 
AATGTTACTG GACAGCCACA TGACTTTCAC CCGGCCTATA GAATTCCCGG ATACATGGAA 
CAGTTGTATT CTCTTCAACG AACTAATTCA GCTTCATCTT TTCACGATCC CTATGTCAAT 
TGTGCATCAG CATTCCATCT TGCCGGACTT GGTTTGGGAT CAGCTGATTT TTTGGGCAGC 
CGAGGTTTGA GCTCTTTGGG TGAACTGCAT AATGCTGCTG TGGCAGCAGC AGCAGCCGGA 
TCTCTCGCAA GTACTGATTT TCACTTCAGT GTTGATGGAA ACAGGCGGTT GGGTAGCCCT 
CGACCACCAG GAGGAAGTAT ACGTGCCAGC ATAAGCCGCA AACGAGCACT TTCGTCATCG 
CCTTATTCCG ATTCATTTGA TATTAATTCA ATGATTCGAT TTTCGCCAAA CTCTTTAGCC 
ACAATTATGA ACGGTTCTCG AGGAAGCAGC GCGGCTAGTG GTTCTTACGG TCATATTTCT 
GCTACCGCCC TTAATCCCAT GTCTCATGTT CATTCAACCC GTCTGCAACA GATACAAGCT 
CATTTATTGC GTGCAAGTGC AGGTCTGTTA AATCCGATGA CACCACAACA AGTTGCTGCA 
TCTGGATTTT CTATAGGGCA TATGCCAACC TCTGCTTCTT TGAGAGTGAA TGACGTACAT 
CCTAATCTAA GCGATTCACA TATCCAGATA ACAACATCCC CAACTGTTAC AAAGGATGTA 
AGCCAGGTCC CAGCGGCAGC ATTCTCGTTG AAGAACTTAG ATGATGCAAG AGAGAAAAAG 
GGACCCTTTA AAGATGTTGT CCCCGAGCAG CCTTCATCGA CATCTGGCGG CGTAGCACAA 
GTAGAAGCCG ACAGTGCGTC TTCACAGTTG TCTGACCGTT GTTACAACAA CGTGGTAAAC 
AATATTACAG GCATTCCTGG TGACGTTAAA GTAAACAGCC GCTTGGATGA GTATATTAAT 
TGCGGATCAA TTTCGATTCC GTCAAATGAA TATGATTGTG CTAATGCTGA TACTACGGAT 
ATCAAGGATG AACCCGGAGA TTTCATTGAA ACAAATTGTC ATTGGCGTAG CTGTCGCATT 
GAGTTCATTA CGCAAGATGA ACTTGTTAAA CATATAAATA ATGATCATAT TCAAACAAAC 
AAGAAAGCTT TTGTCTGTCG ATGGGAAGAT TGTACACGCG GTGAAAAACC ATTTAAAGCG 
CAGTATATGC TTGTTGTGCA TATGCGGCGT CATACCGGTG AAAAGCCACA CAAATGCACG 
GAGAAAAACC ATACACTTGC GAGTATCCGG GATGTAGCAA AGCCTTTAGT AATGCTAGTG 
ATCGCGCAAA GCATCAAAAT CGAACACACA GTAATGAGAA ACCGTACATT TGTAAAGCAC 
CTGGATGCAC AAAACGTTAC ACCGACCCGA GCTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ci ( NM_001258473.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245402.1 Drosophila melanogaster cubitus interruptus (ci), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00130
Clone ID Related Accession (Same CDS sequence)	NM_001258473.1
Accession Version	NM_001258473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cubitus interruptus, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACAGT TGTATTCTCT TCAACGAACT AATTCAGCTT CATCTTTTCA CGATCCCTAT 
GTCAATTGTG CATCAGCATT CCATCTTGCC GGACTTGGTT TGGGATCAGC TGATTTTTTG 
GGCAGCCGAG GTTTGAGCTC TTTGGGTGAA CTGCATAATG CTGCTGTGGC AGCAGCAGCA 
GCCGGATCTC TCGCAAGTAC TGATTTTCAC TTCAGTGTTG ATGGAAACAG GCGGTTGGGT 
AGCCCTCGAC CACCAGGAGG AAGTATACGT GCCAGCATAA GCCGCAAACG AGCACTTTCG 
TCATCGCCTT ATTCCGATTC ATTTGATATT AATTCAATGA TTCGATTTTC GCCAAACTCT 
TTAGCCACAA TTATGAACGG TTCTCGAGGA AGCAGCGCGG CTAGTGGTTC TTACGGTCAT 
ATTTCTGCTA CCGCCCTTAA TCCCATGTCT CATGTTCATT CAACCCGTCT GCAACAGATA 
CAAGCTCATT TATTGCGTGC AAGTGCAGGT CTGTTAAATC CGATGACACC ACAACAAGTT 
GCTGCATCTG GATTTTCTAT AGGGCATATG CCAACCTCTG CTTCTTTGAG AGTGAATGAC 
GTACATCCTA ATCTAAGCGA TTCACATATC CAGATAACAA CATCCCCAAC TGTTACAAAG 
GATGTAAGCC AGGTCCCAGC GGCAGCATTC TCGTTGAAGA ACTTAGATGA TGCAAGAGAG 
AAAAAGGGAC CCTTTAAAGA TGTTGTCCCC GAGCAGCCTT CATCGACATC TGGCGGCGTA 
GCACAAGTAG AAGCCGACAG TGCGTCTTCA CAGTTGTCTG ACCGTTGTTA CAACAACGTG 
GTAAACAATA TTACAGGCAT TCCTGGTGAC GTTAAAGTAA ACAGCCGCTT GGATGAGTAT 
ATTAATTGCG GATCAATTTC GATTCCGTCA AATGAATATG ATTGTGCTAA TGCTGATACT 
ACGGATATCA AGGATGAACC CGGAGATTTC ATTGAAACAA ATTGTCATTG GCGTAGCTGT 
CGCATTGAGT TCATTACGCA AGATGAACTT GTTAAACATA TAAATAATGA TCATATTCAA 
ACAAACAAGA AAGCTTTTGT CTGTCGATGG GAAGATTGTA CACGCGGTGA AAAACCATTT 
AAAGCGCAGT ATATGCTTGT TGTGCATATG CGGCGTCATA CCGGTGAAAA GCCACACAAA 
TGCACGTTTG AAGGCTGCTT TAAGGCATAT TCACGTTTGG AAAACCTGAA AACTCATTTA 
CGGTCACACA CAGGAGAAAA ACCATACACT TGCGAGTATC CGGGATGTAG CAAAGCCTTT 
AGTAATGCTA GTGATCGCGC AAAGCATCAA AATCGAACAC ACAGTAATGA GAAACCGTAC 
ATTTGTAAAG CACCTGGATG CACAAAACGT TACACCGACC CGAGCTCTTT GAGAAAACAT 
GTAAAAACAG TCCATGGAGC TGAATTTTAT GCAAATAAAA AACATAAGGG GTTGCCTCTA 
AATGACGCAA ACTCTCGGTT ACAACAAAAC AACAGTCGGC ATAATCTTCA AGAGCACAAT 
ATCGACTCAA GCCCTTGCAG TGAAGACTCA CATTTGGGAA AAATGTTAGG CACGTCTAGT 
CCTAGCATTA AATCTGAATC AGATATAAGC TCATCAAACC ATCACTTAGT AAATGGAGTT 
CGAGCATCTG ACAGTTTGTT AACATATTCA CCTGATGATC TTGCTGAAAA TCTTAATTTG 
GATGATGGCT GGAATTGTGA CGATGACGTG GACGTGGCAG ACCTACCAAT TGTGTTACGT 
GCTATGGTAA ATATTGGTAA CGGAAATGCA TCCGCCTCGA CTATTGGAGG TTCTGTTTTG 
GCTAGGCAAC GGTTTAGAGG TCGTCTTCAA ACTAAAGGTA TAAATTCAAG TACGATTATG 
CTTTGCAACA TTCCAGAAAG CAATCGTACC TTTGGAATTA GCGAACTTAA TCAGCGTATT 
ACAGAACTGA AAATGGAGCC AGGTACTGAT GCTGAAATTA AAATTCCTAA GCTCCCAAAC 
ACGACCATAG GAGGATATAC GGAAGACCCT TTACAAAACC AAACAAGTTT TCGTAATACA 
GTATCGAATA AGCAAGGAAC TGTTTCCGGA TCGATACAAG GACAGTTTCG TCGCGATAGT 
CAAAATTCCA CTGCAAGCAC TTATTACGGT AGCATGCAAA GTCGTCGTAG CAGTCAATCA 
TCCCAGGTGT CTTCTATACC TACCATGCGA CCGAACCCCT CGTGTAACTC GACTGCATCC 
TTTTATGATC CCATTTCACC AGGGTGCTCA CGGCGCTCTA GCCAAATGTC AAATGGTGCT 
AACTGCAATT CATTTACTTC CACATCAGGA CTACCCGTAT TAAATAAGGA ATCGAACAAA 
AGCTTAAATG CATGTATTAA TAAACCTAAT ATCGGGGTTC AGGGTGTTGG AATTTACAAT 
AGCAGTCTAC CCCCACCTCC ATCGTCCCAC TTAATTGCAA CAAACTTAAA GCGCTTACAG 
CGGAAAGATT CAGAATATCA CAATTTCACT AGCGGGCGAT TTTCTGTTCC TAGTTATATG 
CATTCTTTAC ACATAAAAAA CAACAAACCA GTCGGAGAAA ACGAATTTGA TAAAGCAATA 
GCGAGTAATG CACGACGTCA AACTGATCCA GTGCCAAACA TAAACTTAGA TCCATTAACA 
AATATTTCTC GGTTCAGTAC AACACCGCAT AGCTTTGATA TTAATGTCGG AAAGACTAAT 
AATATAGCAT CGTCAATTAA TAAGGACAAT TTAAGAAAGG ATCTGTTCAC TGTTTCAATT 
AAGGCTGACA TGGCAATGAC TAGTGACCAA CATCCAAATG AAAGAATAAA TTTGGACGAA 
GTTGAAGAAC TTATACTTCC CGATGAAATG CTTCAGTACT TAAACTTGGT AAAAGACGAC 
ACAAATCATT TGGAAAAAGA ACATCAAGCT GTACCTGTGG GGTCAAATGT ATCCGAAACT 
ATAGCTTCAA ACCACTACCG AGAACAATCA AATATATATT ACACCAATAA ACAAATTTTA 
ACACCGCCTT CTAACGTTGA TATACAACCA AATACAACAA AGTTTACAGT ACAAGACAAA 
TTTGCTATGA CGGCGGTCGG GGGTTCATTC AGTCAAAGAG AATTAAGTAC CCTAGCTGTA 
CCAAATGAAC ATGGGCATGC CAAATGCGAA AGCTTCCATC ACCAGTCTCA AAAATATATG 
AACACTGACA TTGGCTCCAA GCAGCAATCA GCATTACCGT CAGCTCATCA ACGGCAAACA 
GAAAAATCAA ATTATAATCA AATCATAGAC TCATCGATGA CTAGCTTGCC AGAACTTAAT 
GTAGATTCAA TATATCCACG AAACGAAACA GAAAATATAT TTAAAGTACA CGGAGATCAT 
GATAATGAAA TCCAATGCGG CATTATAAGT CAATCTCAAA TGTCACCTTC CACTAATTTG 
AATAATGATG GTCAGTTTTC TACCGTAAAT ATGCAACCAA TAACAACATC CAAATTATTT 
CCCCCTGAAC CACAAAAAAT AGTTTGTGAC ACCCAAGCGA GTAATACTTC GGTTATGCAT 
TTAGATACAT ATCAGCGTAC TCTGGAATAT GTACAAAGTT GTCAAAACTG GATGGAGACG 
AACAACACAT CTACCAATCA AATACAAAGC CTCCCGGGAA TGCCGGTAAA CAATACATTG 
TTTCCCGATG TTAGCAGCTC TACACATCCA TATCATGGGA CAAATATGGT GATCAACGAT 
ATGACTACAT CCCTTACTTC CTTACTCGAG GAAAATAGAT ACCTTCAAAT GATGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245402.1
CDS	1068..4907
Translation	MEQLYSLQRTNSASSFHDPYVNCASAFHLAGLGLGSADFLGSRGLSSLGELHNAAVAAAAAGSLASTDFHFSVDGNRRLGSPRPPGGSIRASISRKRALSSSPYSDSFDINSMIRFSPNSLATIMNGSRGSSAASGSYGHISATALNPMSHVHSTRLQQIQAHLLRASAGLLNPMTPQQVAASGFSIGHMPTSASLRVNDVHPNLSDSHIQITTSPTVTKDVSQVPAAAFSLKNLDDAREKKGPFKDVVPEQPSSTSGGVAQVEADSASSQLSDRCYNNVVNNITGIPGDVKVNSRLDEYINCGSISIPSNEYDCANADTTDIKDEPGDFIETNCHWRSCRIEFITQDELVKHINNDHIQTNKKAFVCRWEDCTRGEKPFKAQYMLVVHMRRHTGEKPHKCTFEGCFKAYSRLENLKTHLRSHTGEKPYTCEYPGCSKAFSNASDRAKHQNRTHSNEKPYICKAPGCTKRYTDPSSLRKHVKTVHGAEFYANKKHKGLPLNDANSRLQQNNSRHNLQEHNIDSSPCSEDSHLGKMLGTSSPSIKSESDISSSNHHLVNGVRASDSLLTYSPDDLAENLNLDDGWNCDDDVDVADLPIVLRAMVNIGNGNASASTIGGSVLARQRFRGRLQTKGINSSTIMLCNIPESNRTFGISELNQRITELKMEPGTDAEIKIPKLPNTTIGGYTEDPLQNQTSFRNTVSNKQGTVSGSIQGQFRRDSQNSTASTYYGSMQSRRSSQSSQVSSIPTMRPNPSCNSTASFYDPISPGCSRRSSQMSNGANCNSFTSTSGLPVLNKESNKSLNACINKPNIGVQGVGIYNSSLPPPPSSHLIATNLKRLQRKDSEYHNFTSGRFSVPSYMHSLHIKNNKPVGENEFDKAIASNARRQTDPVPNINLDPLTNISRFSTTPHSFDINVGKTNNIASSINKDNLRKDLFTVSIKADMAMTSDQHPNERINLDEVEELILPDEMLQYLNLVKDDTNHLEKEHQAVPVGSNVSETIASNHYREQSNIYYTNKQILTPPSNVDIQPNTTKFTVQDKFAMTAVGGSFSQRELSTLAVPNEHGHAKCESFHHQSQKYMNTDIGSKQQSALPSAHQRQTEKSNYNQIIDSSMTSLPELNVDSIYPRNETENIFKVHGDHDNEIQCGIISQSQMSPSTNLNNDGQFSTVNMQPITTSKLFPPEPQKIVCDTQASNTSVMHLDTYQRTLEYVQSCQNWMETNNTSTNQIQSLPGMPVNNTLFPDVSSSTHPYHGTNMVINDMTTSLTSLLEENRYLQMMQ

Target ORF information:

RefSeq Version	NM_001258473.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cubitus interruptus (ci), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258473.1

ORF Insert Sequence:

ATGGAACAGT TGTATTCTCT TCAACGAACT AATTCAGCTT CATCTTTTCA CGATCCCTAT 
GTCAATTGTG CATCAGCATT CCATCTTGCC GGACTTGGTT TGGGATCAGC TGATTTTTTG 
GGCAGCCGAG GTTTGAGCTC TTTGGGTGAA CTGCATAATG CTGCTGTGGC AGCAGCAGCA 
GCCGGATCTC TCGCAAGTAC TGATTTTCAC TTCAGTGTTG ATGGAAACAG GCGGTTGGGT 
AGCCCTCGAC CACCAGGAGG AAGTATACGT GCCAGCATAA GCCGCAAACG AGCACTTTCG 
TCATCGCCTT ATTCCGATTC ATTTGATATT AATTCAATGA TTCGATTTTC GCCAAACTCT 
TTAGCCACAA TTATGAACGG TTCTCGAGGA AGCAGCGCGG CTAGTGGTTC TTACGGTCAT 
ATTTCTGCTA CCGCCCTTAA TCCCATGTCT CATGTTCATT CAACCCGTCT GCAACAGATA 
CAAGCTCATT TATTGCGTGC AAGTGCAGGT CTGTTAAATC CGATGACACC ACAACAAGTT 
GCTGCATCTG GATTTTCTAT AGGGCATATG CCAACCTCTG CTTCTTTGAG AGTGAATGAC 
GTACATCCTA ATCTAAGCGA TTCACATATC CAGATAACAA CATCCCCAAC TGTTACAAAG 
GATGTAAGCC AGGTCCCAGC GGCAGCATTC TCGTTGAAGA ACTTAGATGA TGCAAGAGAG 
AAAAAGGGAC CCTTTAAAGA TGTTGTCCCC GAGCAGCCTT CATCGACATC TGGCGGCGTA 
GCACAAGTAG AAGCCGACAG TGCGTCTTCA CAGTTGTCTG ACCGTTGTTA CAACAACGTG 
GTAAACAATA TTACAGGCAT TCCTGGTGAC GTTAAAGTAA ACAGCCGCTT GGATGAGTAT 
ATTAATTGCG GATCAATTTC GATTCCGTCA AATGAATATG ATTGTGCTAA TGCTGATACT 
ACGGATATCA AGGATGAACC CGGAGATTTC ATTGAAACAA ATTGTCATTG GCGTAGCTGT 
CGCATTGAGT TCATTACGCA AGATGAACTT GTTAAACATA TAAATAATGA TCATATTCAA 
ACAAACAAGA AAGCTTTTGT CTGTCGATGG GAAGATTGTA CACGCGGTGA AAAACCATTT 
AAAGCGCAGT ATATGCTTGT TGTGCATATG CGGCGTCATA CCGGTGAAAA GCCACACAAA 
TGCACGTTTG AAGGCTGCTT TAAGGCATAT TCACGTTTGG AAAACCTGAA AACTCATTTA 
CGGTCACACA CAGGAGAAAA ACCATACACT TGCGAGTATC CGGGATGTAG CAAAGCCTTT 
AGTAATGCTA GTGATCGCGC AAAGCATCAA AATCGAACAC ACAGTAATGA GAAACCGTAC 
ATTTGTAAAG CACCTGGATG CACAAAACGT TACACCGACC CGAGCTCTTT GAGAAAACAT 
GTAAAAACAG TCCATGGAGC TGAATTTTAT GCAAATAAAA AACATAAGGG GTTGCCTCTA 
AATGACGCAA ACTCTCGGTT ACAACAAAAC AACAGTCGGC ATAATCTTCA AGAGCACAAT 
ATCGACTCAA GCCCTTGCAG TGAAGACTCA CATTTGGGAA AAATGTTAGG CACGTCTAGT 
CCTAGCATTA AATCTGAATC AGATATAAGC TCATCAAACC ATCACTTAGT AAATGGAGTT 
CGAGCATCTG ACAGTTTGTT AACATATTCA CCTGATGATC TTGCTGAAAA TCTTAATTTG 
GATGATGGCT GGAATTGTGA CGATGACGTG GACGTGGCAG ACCTACCAAT TGTGTTACGT 
GCTATGGTAA ATATTGGTAA CGGAAATGCA TCCGCCTCGA CTATTGGAGG TTCTGTTTTG 
GCTAGGCAAC GGTTTAGAGG TCGTCTTCAA ACTAAAGGTA TAAATTCAAG TACGATTATG 
CTTTGCAACA TTCCAGAAAG CAATCGTACC TTTGGAATTA GCGAACTTAA TCAGCGTATT 
ACAGAACTGA AAATGGAGCC AGGTACTGAT GCTGAAATTA AAATTCCTAA GCTCCCAAAC 
ACGACCATAG GAGGATATAC GGAAGACCCT TTACAAAACC AAACAAGTTT TCGTAATACA 
GTATCGAATA AGCAAGGAAC TGTTTCCGGA TCGATACAAG GACAGTTTCG TCGCGATAGT 
CAAAATTCCA CTGCAAGCAC TTATTACGGT AGCATGCAAA GTCGTCGTAG CAGTCAATCA 
TCCCAGGTGT CTTCTATACC TACCATGCGA CCGAACCCCT CGTGTAACTC GACTGCATCC 
TTTTATGATC CCATTTCACC AGGGTGCTCA CGGCGCTCTA GCCAAATGTC AAATGGTGCT 
AACTGCAATT CATTTACTTC CACATCAGGA CTACCCGTAT TAAATAAGGA ATCGAACAAA 
AGCTTAAATG CATGTATTAA TAAACCTAAT ATCGGGGTTC AGGGTGTTGG AATTTACAAT 
AGCAGTCTAC CCCCACCTCC ATCGTCCCAC TTAATTGCAA CAAACTTAAA GCGCTTACAG 
CGGAAAGATT CAGAATATCA CAATTTCACT AGCGGGCGAT TTTCTGTTCC TAGTTATATG 
CATTCTTTAC ACATAAAAAA CAACAAACCA GTCGGAGAAA ACGAATTTGA TAAAGCAATA 
GCGAGTAATG CACGACGTCA AACTGATCCA GTGCCAAACA TAAACTTAGA TCCATTAACA 
AATATTTCTC GGTTCAGTAC AACACCGCAT AGCTTTGATA TTAATGTCGG AAAGACTAAT 
AATATAGCAT CGTCAATTAA TAAGGACAAT TTAAGAAAGG ATCTGTTCAC TGTTTCAATT 
AAGGCTGACA TGGCAATGAC TAGTGACCAA CATCCAAATG AAAGAATAAA TTTGGACGAA 
GTTGAAGAAC TTATACTTCC CGATGAAATG CTTCAGTACT TAAACTTGGT AAAAGACGAC 
ACAAATCATT TGGAAAAAGA ACATCAAGCT GTACCTGTGG GGTCAAATGT ATCCGAAACT 
ATAGCTTCAA ACCACTACCG AGAACAATCA AATATATATT ACACCAATAA ACAAATTTTA 
ACACCGCCTT CTAACGTTGA TATACAACCA AATACAACAA AGTTTACAGT ACAAGACAAA 
TTTGCTATGA CGGCGGTCGG GGGTTCATTC AGTCAAAGAG AATTAAGTAC CCTAGCTGTA 
CCAAATGAAC ATGGGCATGC CAAATGCGAA AGCTTCCATC ACCAGTCTCA AAAATATATG 
AACACTGACA TTGGCTCCAA GCAGCAATCA GCATTACCGT CAGCTCATCA ACGGCAAACA 
GAAAAATCAA ATTATAATCA AATCATAGAC TCATCGATGA CTAGCTTGCC AGAACTTAAT 
GTAGATTCAA TATATCCACG AAACGAAACA GAAAATATAT TTAAAGTACA CGGAGATCAT 
GATAATGAAA TCCAATGCGG CATTATAAGT CAATCTCAAA TGTCACCTTC CACTAATTTG 
AATAATGATG GTCAGTTTTC TACCGTAAAT ATGCAACCAA TAACAACATC CAAATTATTT 
CCCCCTGAAC CACAAAAAAT AGTTTGTGAC ACCCAAGCGA GTAATACTTC GGTTATGCAT 
TTAGATACAT ATCAGCGTAC TCTGGAATAT GTACAAAGTT GTCAAAACTG GATGGAGACG 
AACAACACAT CTACCAATCA AATACAAAGC CTCCCGGGAA TGCCGGTAAA CAATACATTG 
TTTCCCGATG TTAGCAGCTC TACACATCCA TATCATGGGA CAAATATGGT GATCAACGAT 
ATGACTACAT CCCTTACTTC CTTACTCGAG GAAAATAGAT ACCTTCAAAT GATGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ci ( NM_079878.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524617.3 Drosophila melanogaster cubitus interruptus (ci), transcript variant A, mRNA.

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CloneID	OFa00247
Clone ID Related Accession (Same CDS sequence)	NM_079878.5
Accession Version	NM_079878.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cubitus interruptus, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_079878.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCCT ACGCGTTACC TACATATTTT CCTCTTGCGT ATTCTGAATT GCAGTTTTTA 
GCGTCCAGGA GAGCAGCTGC CGTCGCTGCA GCGGCTACTG TTTTACCAGG ATCACCATGC 
ATAAACCAAC ATCACCCAAC TGACGTTTCA AGCTCGGTAA CAGTGCCATC AATTATTCCA 
ACGGGTGGAA CATCAGATTC AATTAAAACT TCAATACAAC CACAAATATG CAATGAAAAC 
ACCCTTCTTG GAAATGCTGG CCACCAGCAC AATCATCAGC CTCAACATGT TCACAATATA 
AATGTTACTG GACAGCCACA TGACTTTCAC CCGGCCTATA GAATTCCCGG ATACATGGAA 
CAGTTGTATT CTCTTCAACG AACTAATTCA GCTTCATCTT TTCACGATCC CTATGTCAAT 
TGTGCATCAG CATTCCATCT TGCCGGACTT GGTTTGGGAT CAGCTGATTT TTTGGGCAGC 
CGAGGTTTGA GCTCTTTGGG TGAACTGCAT AATGCTGCTG TGGCAGCAGC AGCAGCCGGA 
TCTCTCGCAA GTACTGATTT TCACTTCAGT GTTGATGGAA ACAGGCGGTT GGGTAGCCCT 
CGACCACCAG GAGGAAGTAT ACGTGCCAGC ATAAGCCGCA AACGAGCACT TTCGTCATCG 
CCTTATTCCG ATTCATTTGA TATTAATTCA ATGATTCGAT TTTCGCCAAA CTCTTTAGCC 
ACAATTATGA ACGGTTCTCG AGGAAGCAGC GCGGCTAGTG GTTCTTACGG TCATATTTCT 
GCTACCGCCC TTAATCCCAT GTCTCATGTT CATTCAACCC GTCTGCAACA GATACAAGCT 
CATTTATTGC GTGCAAGTGC AGGTCTGTTA AATCCGATGA CACCACAACA AGTTGCTGCA 
TCTGGATTTT CTATAGGGCA TATGCCAACC TCTGCTTCTT TGAGAGTGAA TGACGTACAT 
CCTAATCTAA GCGATTCACA TATCCAGATA ACAACATCCC CAACTGTTAC AAAGGATGTA 
AGCCAGGTCC CAGCGGCAGC ATTCTCGTTG AAGAACTTAG ATGATGCAAG AGAGAAAAAG 
GGACCCTTTA AAGATGTTGT CCCCGAGCAG CCTTCATCGA CATCTGGCGG CGTAGCACAA 
GTAGAAGCCG ACAGTGCGTC TTCACAGTTG TCTGACCGTT GTTACAACAA CGTGGTAAAC 
AATATTACAG GCATTCCTGG TGACGTTAAA GTAAACAGCC GCTTGGATGA GTATATTAAT 
TGCGGATCAA TTTCGATTCC GTCAAATGAA TATGATTGTG CTAATGCTGA TACTACGGAT 
ATCAAGGATG AACCCGGAGA TTTCATTGAA ACAAATTGTC ATTGGCGTAG CTGTCGCATT 
GAGTTCATTA CGCAAGATGA ACTTGTTAAA CATATAAATA ATGATCATAT TCAAACAAAC 
AAGAAAGCTT TTGTCTGTCG ATGGGAAGAT TGTACACGCG GTGAAAAACC ATTTAAAGCG 
CAGTATATGC TTGTTGTGCA TATGCGGCGT CATACCGGTG AAAAGCCACA CAAATGCACG 
TTTGAAGGCT GCTTTAAGGC ATATTCACGT TTGGAAAACC TGAAAACTCA TTTACGGTCA 
CACACAGGAG AAAAACCATA CACTTGCGAG TATCCGGGAT GTAGCAAAGC CTTTAGTAAT 
GCTAGTGATC GCGCAAAGCA TCAAAATCGA ACACACAGTA ATGAGAAACC GTACATTTGT 
AAAGCACCTG GATGCACAAA ACGTTACACC GACCCGAGCT CTTTGAGAAA ACATGTAAAA 
ACAGTCCATG GAGCTGAATT TTATGCAAAT AAAAAACATA AGGGGTTGCC TCTAAATGAC 
GCAAACTCTC GGTTACAACA AAACAACAGT CGGCATAATC TTCAAGAGCA CAATATCGAC 
TCAAGCCCTT GCAGTGAAGA CTCACATTTG GGAAAAATGT TAGGCACGTC TAGTCCTAGC 
ATTAAATCTG AATCAGATAT AAGCTCATCA AACCATCACT TAGTAAATGG AGTTCGAGCA 
TCTGACAGTT TGTTAACATA TTCACCTGAT GATCTTGCTG AAAATCTTAA TTTGGATGAT 
GGCTGGAATT GTGACGATGA CGTGGACGTG GCAGACCTAC CAATTGTGTT ACGTGCTATG 
GTAAATATTG GTAACGGAAA TGCATCCGCC TCGACTATTG GAGGTTCTGT TTTGGCTAGG 
CAACGGTTTA GAGGTCGTCT TCAAACTAAA GGTATAAATT CAAGTACGAT TATGCTTTGC 
AACATTCCAG AAAGCAATCG TACCTTTGGA ATTAGCGAAC TTAATCAGCG TATTACAGAA 
CTGAAAATGG AGCCAGGTAC TGATGCTGAA ATTAAAATTC CTAAGCTCCC AAACACGACC 
ATAGGAGGAT ATACGGAAGA CCCTTTACAA AACCAAACAA GTTTTCGTAA TACAGTATCG 
AATAAGCAAG GAACTGTTTC CGGATCGATA CAAGGACAGT TTCGTCGCGA TAGTCAAAAT 
TCCACTGCAA GCACTTATTA CGGTAGCATG CAAAGTCGTC GTAGCAGTCA ATCATCCCAG 
GTGTCTTCTA TACCTACCAT GCGACCGAAC CCCTCGTGTA ACTCGACTGC ATCCTTTTAT 
GATCCCATTT CACCAGGGTG CTCACGGCGC TCTAGCCAAA TGTCAAATGG TGCTAACTGC 
AATTCATTTA CTTCCACATC AGGACTACCC GTATTAAATA AGGAATCGAA CAAAAGCTTA 
AATGCATGTA TTAATAAACC TAATATCGGG GTTCAGGGTG TTGGAATTTA CAATAGCAGT 
CTACCCCCAC CTCCATCGTC CCACTTAATT GCAACAAACT TAAAGCGCTT ACAGCGGAAA 
GATTCAGAAT ATCACAATTT CACTAGCGGG CGATTTTCTG TTCCTAGTTA TATGCATTCT 
TTACACATAA AAAACAACAA ACCAGTCGGA GAAAACGAAT TTGATAAAGC AATAGCGAGT 
AATGCACGAC GTCAAACTGA TCCAGTGCCA AACATAAACT TAGATCCATT AACAAATATT 
TCTCGGTTCA GTACAACACC GCATAGCTTT GATATTAATG TCGGAAAGAC TAATAATATA 
GCATCGTCAA TTAATAAGGA CAATTTAAGA AAGGATCTGT TCACTGTTTC AATTAAGGCT 
GACATGGCAA TGACTAGTGA CCAACATCCA AATGAAAGAA TAAATTTGGA CGAAGTTGAA 
GAACTTATAC TTCCCGATGA AATGCTTCAG TACTTAAACT TGGTAAAAGA CGACACAAAT 
CATTTGGAAA AAGAACATCA AGCTGTACCT GTGGGGTCAA ATGTATCCGA AACTATAGCT 
TCAAACCACT ACCGAGAACA ATCAAATATA TATTACACCA ATAAACAAAT TTTAACACCG 
CCTTCTAACG TTGATATACA ACCAAATACA ACAAAGTTTA CAGTACAAGA CAAATTTGCT 
ATGACGGCGG TCGGGGGTTC ATTCAGTCAA AGAGAATTAA GTACCCTAGC TGTACCAAAT 
GAACATGGGC ATGCCAAATG CGAAAGCTTC CATCACCAGT CTCAAAAATA TATGAACACT 
GACATTGGCT CCAAGCAGCA ATCAGCATTA CCGTCAGCTC ATCAACGGCA AACAGAAAAA 
TCAAATTATA ATCAAATCAT AGACTCATCG ATGACTAGCT TGCCAGAACT TAATGTAGAT 
TCAATATATC CACGAAACGA AACAGAAAAT ATATTTAAAG TACACGGAGA TCATGATAAT 
GAAATCCAAT GCGGCATTAT AAGTCAATCT CAAATGTCAC CTTCCACTAA TTTGAATAAT 
GATGGTCAGT TTTCTACCGT AAATATGCAA CCAATAACAA CATCCAAATT ATTTCCCCCT 
GAACCACAAA AAATAGTTTG TGACACCCAA GCGAGTAATA CTTCGGTTAT GCATTTAGAT 
ACATATCAGC GTACTCTGGA ATATGTACAA AGTTGTCAAA ACTGGATGGA GACGAACAAC 
ACATCTACCA ATCAAATACA AAGCCTCCCG GGAATGCCGG TAAACAATAC ATTGTTTCCC 
GATGTTAGCA GCTCTACACA TCCATATCAT GGGACAAATA TGGTGATCAA CGATATGACT 
ACATCCCTTA CTTCCTTACT CGAGGAAAAT AGATACCTTC AAATGATGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524617.3
CDS	464..4657
Translation	MDAYALPTYFPLAYSELQFLASRRAAAVAAAATVLPGSPCINQHHPTDVSSSVTVPSIIPTGGTSDSIKTSIQPQICNENTLLGNAGHQHNHQPQHVHNINVTGQPHDFHPAYRIPGYMEQLYSLQRTNSASSFHDPYVNCASAFHLAGLGLGSADFLGSRGLSSLGELHNAAVAAAAAGSLASTDFHFSVDGNRRLGSPRPPGGSIRASISRKRALSSSPYSDSFDINSMIRFSPNSLATIMNGSRGSSAASGSYGHISATALNPMSHVHSTRLQQIQAHLLRASAGLLNPMTPQQVAASGFSIGHMPTSASLRVNDVHPNLSDSHIQITTSPTVTKDVSQVPAAAFSLKNLDDAREKKGPFKDVVPEQPSSTSGGVAQVEADSASSQLSDRCYNNVVNNITGIPGDVKVNSRLDEYINCGSISIPSNEYDCANADTTDIKDEPGDFIETNCHWRSCRIEFITQDELVKHINNDHIQTNKKAFVCRWEDCTRGEKPFKAQYMLVVHMRRHTGEKPHKCTFEGCFKAYSRLENLKTHLRSHTGEKPYTCEYPGCSKAFSNASDRAKHQNRTHSNEKPYICKAPGCTKRYTDPSSLRKHVKTVHGAEFYANKKHKGLPLNDANSRLQQNNSRHNLQEHNIDSSPCSEDSHLGKMLGTSSPSIKSESDISSSNHHLVNGVRASDSLLTYSPDDLAENLNLDDGWNCDDDVDVADLPIVLRAMVNIGNGNASASTIGGSVLARQRFRGRLQTKGINSSTIMLCNIPESNRTFGISELNQRITELKMEPGTDAEIKIPKLPNTTIGGYTEDPLQNQTSFRNTVSNKQGTVSGSIQGQFRRDSQNSTASTYYGSMQSRRSSQSSQVSSIPTMRPNPSCNSTASFYDPISPGCSRRSSQMSNGANCNSFTSTSGLPVLNKESNKSLNACINKPNIGVQGVGIYNSSLPPPPSSHLIATNLKRLQRKDSEYHNFTSGRFSVPSYMHSLHIKNNKPVGENEFDKAIASNARRQTDPVPNINLDPLTNISRFSTTPHSFDINVGKTNNIASSINKDNLRKDLFTVSIKADMAMTSDQHPNERINLDEVEELILPDEMLQYLNLVKDDTNHLEKEHQAVPVGSNVSETIASNHYREQSNIYYTNKQILTPPSNVDIQPNTTKFTVQDKFAMTAVGGSFSQRELSTLAVPNEHGHAKCESFHHQSQKYMNTDIGSKQQSALPSAHQRQTEKSNYNQIIDSSMTSLPELNVDSIYPRNETENIFKVHGDHDNEIQCGIISQSQMSPSTNLNNDGQFSTVNMQPITTSKLFPPEPQKIVCDTQASNTSVMHLDTYQRTLEYVQSCQNWMETNNTSTNQIQSLPGMPVNNTLFPDVSSSTHPYHGTNMVINDMTTSLTSLLEENRYLQMMQ

Target ORF information:

RefSeq Version	NM_079878.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cubitus interruptus (ci), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079878.5

ORF Insert Sequence:

ATGGACGCCT ACGCGTTACC TACATATTTT CCTCTTGCGT ATTCTGAATT GCAGTTTTTA 
GCGTCCAGGA GAGCAGCTGC CGTCGCTGCA GCGGCTACTG TTTTACCAGG ATCACCATGC 
ATAAACCAAC ATCACCCAAC TGACGTTTCA AGCTCGGTAA CAGTGCCATC AATTATTCCA 
ACGGGTGGAA CATCAGATTC AATTAAAACT TCAATACAAC CACAAATATG CAATGAAAAC 
ACCCTTCTTG GAAATGCTGG CCACCAGCAC AATCATCAGC CTCAACATGT TCACAATATA 
AATGTTACTG GACAGCCACA TGACTTTCAC CCGGCCTATA GAATTCCCGG ATACATGGAA 
CAGTTGTATT CTCTTCAACG AACTAATTCA GCTTCATCTT TTCACGATCC CTATGTCAAT 
TGTGCATCAG CATTCCATCT TGCCGGACTT GGTTTGGGAT CAGCTGATTT TTTGGGCAGC 
CGAGGTTTGA GCTCTTTGGG TGAACTGCAT AATGCTGCTG TGGCAGCAGC AGCAGCCGGA 
TCTCTCGCAA GTACTGATTT TCACTTCAGT GTTGATGGAA ACAGGCGGTT GGGTAGCCCT 
CGACCACCAG GAGGAAGTAT ACGTGCCAGC ATAAGCCGCA AACGAGCACT TTCGTCATCG 
CCTTATTCCG ATTCATTTGA TATTAATTCA ATGATTCGAT TTTCGCCAAA CTCTTTAGCC 
ACAATTATGA ACGGTTCTCG AGGAAGCAGC GCGGCTAGTG GTTCTTACGG TCATATTTCT 
GCTACCGCCC TTAATCCCAT GTCTCATGTT CATTCAACCC GTCTGCAACA GATACAAGCT 
CATTTATTGC GTGCAAGTGC AGGTCTGTTA AATCCGATGA CACCACAACA AGTTGCTGCA 
TCTGGATTTT CTATAGGGCA TATGCCAACC TCTGCTTCTT TGAGAGTGAA TGACGTACAT 
CCTAATCTAA GCGATTCACA TATCCAGATA ACAACATCCC CAACTGTTAC AAAGGATGTA 
AGCCAGGTCC CAGCGGCAGC ATTCTCGTTG AAGAACTTAG ATGATGCAAG AGAGAAAAAG 
GGACCCTTTA AAGATGTTGT CCCCGAGCAG CCTTCATCGA CATCTGGCGG CGTAGCACAA 
GTAGAAGCCG ACAGTGCGTC TTCACAGTTG TCTGACCGTT GTTACAACAA CGTGGTAAAC 
AATATTACAG GCATTCCTGG TGACGTTAAA GTAAACAGCC GCTTGGATGA GTATATTAAT 
TGCGGATCAA TTTCGATTCC GTCAAATGAA TATGATTGTG CTAATGCTGA TACTACGGAT 
ATCAAGGATG AACCCGGAGA TTTCATTGAA ACAAATTGTC ATTGGCGTAG CTGTCGCATT 
GAGTTCATTA CGCAAGATGA ACTTGTTAAA CATATAAATA ATGATCATAT TCAAACAAAC 
AAGAAAGCTT TTGTCTGTCG ATGGGAAGAT TGTACACGCG GTGAAAAACC ATTTAAAGCG 
CAGTATATGC TTGTTGTGCA TATGCGGCGT CATACCGGTG AAAAGCCACA CAAATGCACG 
TTTGAAGGCT GCTTTAAGGC ATATTCACGT TTGGAAAACC TGAAAACTCA TTTACGGTCA 
CACACAGGAG AAAAACCATA CACTTGCGAG TATCCGGGAT GTAGCAAAGC CTTTAGTAAT 
GCTAGTGATC GCGCAAAGCA TCAAAATCGA ACACACAGTA ATGAGAAACC GTACATTTGT 
AAAGCACCTG GATGCACAAA ACGTTACACC GACCCGAGCT CTTTGAGAAA ACATGTAAAA 
ACAGTCCATG GAGCTGAATT TTATGCAAAT AAAAAACATA AGGGGTTGCC TCTAAATGAC 
GCAAACTCTC GGTTACAACA AAACAACAGT CGGCATAATC TTCAAGAGCA CAATATCGAC 
TCAAGCCCTT GCAGTGAAGA CTCACATTTG GGAAAAATGT TAGGCACGTC TAGTCCTAGC 
ATTAAATCTG AATCAGATAT AAGCTCATCA AACCATCACT TAGTAAATGG AGTTCGAGCA 
TCTGACAGTT TGTTAACATA TTCACCTGAT GATCTTGCTG AAAATCTTAA TTTGGATGAT 
GGCTGGAATT GTGACGATGA CGTGGACGTG GCAGACCTAC CAATTGTGTT ACGTGCTATG 
GTAAATATTG GTAACGGAAA TGCATCCGCC TCGACTATTG GAGGTTCTGT TTTGGCTAGG 
CAACGGTTTA GAGGTCGTCT TCAAACTAAA GGTATAAATT CAAGTACGAT TATGCTTTGC 
AACATTCCAG AAAGCAATCG TACCTTTGGA ATTAGCGAAC TTAATCAGCG TATTACAGAA 
CTGAAAATGG AGCCAGGTAC TGATGCTGAA ATTAAAATTC CTAAGCTCCC AAACACGACC 
ATAGGAGGAT ATACGGAAGA CCCTTTACAA AACCAAACAA GTTTTCGTAA TACAGTATCG 
AATAAGCAAG GAACTGTTTC CGGATCGATA CAAGGACAGT TTCGTCGCGA TAGTCAAAAT 
TCCACTGCAA GCACTTATTA CGGTAGCATG CAAAGTCGTC GTAGCAGTCA ATCATCCCAG 
GTGTCTTCTA TACCTACCAT GCGACCGAAC CCCTCGTGTA ACTCGACTGC ATCCTTTTAT 
GATCCCATTT CACCAGGGTG CTCACGGCGC TCTAGCCAAA TGTCAAATGG TGCTAACTGC 
AATTCATTTA CTTCCACATC AGGACTACCC GTATTAAATA AGGAATCGAA CAAAAGCTTA 
AATGCATGTA TTAATAAACC TAATATCGGG GTTCAGGGTG TTGGAATTTA CAATAGCAGT 
CTACCCCCAC CTCCATCGTC CCACTTAATT GCAACAAACT TAAAGCGCTT ACAGCGGAAA 
GATTCAGAAT ATCACAATTT CACTAGCGGG CGATTTTCTG TTCCTAGTTA TATGCATTCT 
TTACACATAA AAAACAACAA ACCAGTCGGA GAAAACGAAT TTGATAAAGC AATAGCGAGT 
AATGCACGAC GTCAAACTGA TCCAGTGCCA AACATAAACT TAGATCCATT AACAAATATT 
TCTCGGTTCA GTACAACACC GCATAGCTTT GATATTAATG TCGGAAAGAC TAATAATATA 
GCATCGTCAA TTAATAAGGA CAATTTAAGA AAGGATCTGT TCACTGTTTC AATTAAGGCT 
GACATGGCAA TGACTAGTGA CCAACATCCA AATGAAAGAA TAAATTTGGA CGAAGTTGAA 
GAACTTATAC TTCCCGATGA AATGCTTCAG TACTTAAACT TGGTAAAAGA CGACACAAAT 
CATTTGGAAA AAGAACATCA AGCTGTACCT GTGGGGTCAA ATGTATCCGA AACTATAGCT 
TCAAACCACT ACCGAGAACA ATCAAATATA TATTACACCA ATAAACAAAT TTTAACACCG 
CCTTCTAACG TTGATATACA ACCAAATACA ACAAAGTTTA CAGTACAAGA CAAATTTGCT 
ATGACGGCGG TCGGGGGTTC ATTCAGTCAA AGAGAATTAA GTACCCTAGC TGTACCAAAT 
GAACATGGGC ATGCCAAATG CGAAAGCTTC CATCACCAGT CTCAAAAATA TATGAACACT 
GACATTGGCT CCAAGCAGCA ATCAGCATTA CCGTCAGCTC ATCAACGGCA AACAGAAAAA 
TCAAATTATA ATCAAATCAT AGACTCATCG ATGACTAGCT TGCCAGAACT TAATGTAGAT 
TCAATATATC CACGAAACGA AACAGAAAAT ATATTTAAAG TACACGGAGA TCATGATAAT 
GAAATCCAAT GCGGCATTAT AAGTCAATCT CAAATGTCAC CTTCCACTAA TTTGAATAAT 
GATGGTCAGT TTTCTACCGT AAATATGCAA CCAATAACAA CATCCAAATT ATTTCCCCCT 
GAACCACAAA AAATAGTTTG TGACACCCAA GCGAGTAATA CTTCGGTTAT GCATTTAGAT 
ACATATCAGC GTACTCTGGA ATATGTACAA AGTTGTCAAA ACTGGATGGA GACGAACAAC 
ACATCTACCA ATCAAATACA AAGCCTCCCG GGAATGCCGG TAAACAATAC ATTGTTTCCC 
GATGTTAGCA GCTCTACACA TCCATATCAT GGGACAAATA TGGTGATCAA CGATATGACT 
ACATCCCTTA CTTCCTTACT CGAGGAAAAT AGATACCTTC AAATGATGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ci cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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