betaGlu cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	betaGlu
Entrez Gene ID	43746
Full Name	beta glucuronidase
Synonyms	CG2135,Dmel\CG2135,Glc,Glu,beta-GUS,gus
General protein information	Preferred Names beta glucuronidase Names CG2135 gene product from transcript CG2135-RA CG2135-PA beta-Glucuronidase betaGlu-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100D2-100D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_143636.3	NP_651893.1	beta glucuronidase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following betaGlu gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the betaGlu cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06797	NM_143636.3 Latest version!	Drosophila melanogaster beta glucuronidase (betaGlu), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

betaGlu ( NM_143636.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651893.1 Drosophila melanogaster beta glucuronidase (betaGlu), mRNA.

Price & Availability↑

CloneID	OFa06797
Clone ID Related Accession (Same CDS sequence)	NM_143636.3
Accession Version	NM_143636.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta glucuronidase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143636.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAGTC ATTGGATGCC CGTCACCTGC TTACTGCTTG GCAGTCTCCA GGTTCTATTG 
GCTTACACCG AAAACGACTT CTTGGTGAAC GCCAATCGCA AAGAACTAAC TGATTCCCCT 
TTGTTTGAAT ATGATAATGT CAGAAAGCCC ACACCAACCA AGGGATTGTT GTACCCCAGG 
GATTCGGAGA CCCGTGAAGT GCGGAGCTTG GATGGAATGT GGCAACTGGT TAGGAGTGAT 
CCATATGACC CCTTACAGGG AATTAGGGAA AAGTGGTTTA TGGATGCCTT GAGAAAAACC 
GGAAGGGAGA TTATTTCGAT GCCAGTGCCT GCCTCCTATA ACGACATCAC TACAGATAAC 
TTACGAGATC ACGTTGGAAC CGTATGGTAT GAGCGTACTT TTTTTGTGCC GCGCTCCTGG 
AAGACTATGC AACGCACTTG GTTGCGATTT AGCAGCGTTC ACTATTCGGC CGTGGTATGG 
ATCAACGGAA GGAACGCCAC AAGTCACTCC ATTGGCCACC TACCCTTTGA GTCAGAGATC 
TCGGGACTGC TCAGCTTTGG CGGCGATAAC AGGATCACGG TGATGTGTGA CAATCGTTTG 
AGCAACCGCA CCATTCCTCA GGGTTCTGTT TACAAGGTGG CTACGGATGA CGGCACCGTG 
CCCATCCAGA GTTACACTTT CGATTTTTTT AACTACGCGG GAATCCACCG GAGTGTACAC 
TTGTACACGA CCCCGCTGCT GCACATAAGC GATTTGGAGG TCACCACCCA ACTAACTAAA 
GAAGGATTGG GCCGCATAGA CTACCGCGTG TGGTTGGATG CTAGCAAGGA GGGCCTCCAA 
ATCCAGCCCA TCCAACTTCG GGTGCAACTA AGGGACAAAG ATGGTCATGT GGCCGCCCAG 
CAGATCAACA AGGCGGTCTA CCATGGTACT CTGCTGGTGC CAAATGCAAC TCCCTGGTGG 
CCCTACCTAA TGCATTCCGA TCCCGGCTAC CTGTATAATC TTCAATTCGA GCTGTTTGTG 
GCCAGCAATG AGAAGGAGTT GGAATCTCTG CAGGATACCT ACCGCCTCCC AGTGGGCATA 
CGTAGCTTAA GTTGGGACAA CGACAGCCTG CTGCTGAATG GAAAACCCCT CTATCTGCGG 
GGATTTGGAC GGCACGAGGA CTCCGATATC CGCGGGAAGG GATTGGATAA TGCGCTTCTT 
GCTAGAGATT TTAACCTGCT GAAGTGGACT GGAGCCAATG CATATCGCAC CTCTCACTAT 
CCTTATTCCG AAGAGTCAAT GCAGTTTGCC GATCAGCATG GTATTATGAT TATTGACGAA 
TGCCCTGCTG TCAATATAGA TATCTTCGAG CCGCAGCTAC TGGAGAATCA CATGTCCTCG 
CTGGAGCAAC TGATCCACCG GGACAGAAAC CATCCAAGTG TTGTTGCATG GTCGGTAGCC 
AACGAGCCGA GATCGAACAA GCAGGGAGCC CTTAAATACT TTGAATTCCT GGTAAACTAT 
GTAAGAGAAA TAGCTCACGG ACGACCTCTA ACCGCGGCTA TAAATGCCAA CTCTTCCAGT 
TGCCATTTGG CGCAGTTTCT GGACATCGTG GGTTTCAATC GCTATAACTC TTGGTATCAG 
AACTCGGGCC GTACTGACAT GATACTTAAC CTGTTGACGA TAGAGGCCCA GAGTTGGCGG 
GATAGGTTCG GAAAGCCTGT CATCCAATTT GAGTACGGCG GCGACACTAT GGAGGGCATG 
CACTCACTTC CCGCCTTTAT TTGGTCGGAG GAATACCAGG TTGAGCTCTT CTCTCGCCAT 
TTCAAGGCTT TCGACGAGTT ACGCGGAAGA GGATGGTTTA TTGGAGAGTT TGTTTGGAAC 
TTCGCCGATT TTCGGACCGC GCAGACTATT ACCCGCGTGG GCGGCAACAA AAAGGGAGTC 
TTTACAAGGA ACCGACAACC CAAAGAAGTG GCTCACATTC TTAGGCGGCG GTACTTCGCA 
TTAGCCAAGG AGTTGGATAT GTTTGGGCTG CCTGAGGATC TCACTGTTTA CATTTCCAAG 
CATATCCATA ACGAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651893.1
CDS	91..2151
Translation	MKSHWMPVTCLLLGSLQVLLAYTENDFLVNANRKELTDSPLFEYDNVRKPTPTKGLLYPRDSETREVRSLDGMWQLVRSDPYDPLQGIREKWFMDALRKTGREIISMPVPASYNDITTDNLRDHVGTVWYERTFFVPRSWKTMQRTWLRFSSVHYSAVVWINGRNATSHSIGHLPFESEISGLLSFGGDNRITVMCDNRLSNRTIPQGSVYKVATDDGTVPIQSYTFDFFNYAGIHRSVHLYTTPLLHISDLEVTTQLTKEGLGRIDYRVWLDASKEGLQIQPIQLRVQLRDKDGHVAAQQINKAVYHGTLLVPNATPWWPYLMHSDPGYLYNLQFELFVASNEKELESLQDTYRLPVGIRSLSWDNDSLLLNGKPLYLRGFGRHEDSDIRGKGLDNALLARDFNLLKWTGANAYRTSHYPYSEESMQFADQHGIMIIDECPAVNIDIFEPQLLENHMSSLEQLIHRDRNHPSVVAWSVANEPRSNKQGALKYFEFLVNYVREIAHGRPLTAAINANSSSCHLAQFLDIVGFNRYNSWYQNSGRTDMILNLLTIEAQSWRDRFGKPVIQFEYGGDTMEGMHSLPAFIWSEEYQVELFSRHFKAFDELRGRGWFIGEFVWNFADFRTAQTITRVGGNKKGVFTRNRQPKEVAHILRRRYFALAKELDMFGLPEDLTVYISKHIHNEL

Target ORF information:

RefSeq Version	NM_143636.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta glucuronidase (betaGlu), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143636.3

ORF Insert Sequence:

ATGAAAAGTC ATTGGATGCC CGTCACCTGC TTACTGCTTG GCAGTCTCCA GGTTCTATTG 
GCTTACACCG AAAACGACTT CTTGGTGAAC GCCAATCGCA AAGAACTAAC TGATTCCCCT 
TTGTTTGAAT ATGATAATGT CAGAAAGCCC ACACCAACCA AGGGATTGTT GTACCCCAGG 
GATTCGGAGA CCCGTGAAGT GCGGAGCTTG GATGGAATGT GGCAACTGGT TAGGAGTGAT 
CCATATGACC CCTTACAGGG AATTAGGGAA AAGTGGTTTA TGGATGCCTT GAGAAAAACC 
GGAAGGGAGA TTATTTCGAT GCCAGTGCCT GCCTCCTATA ACGACATCAC TACAGATAAC 
TTACGAGATC ACGTTGGAAC CGTATGGTAT GAGCGTACTT TTTTTGTGCC GCGCTCCTGG 
AAGACTATGC AACGCACTTG GTTGCGATTT AGCAGCGTTC ACTATTCGGC CGTGGTATGG 
ATCAACGGAA GGAACGCCAC AAGTCACTCC ATTGGCCACC TACCCTTTGA GTCAGAGATC 
TCGGGACTGC TCAGCTTTGG CGGCGATAAC AGGATCACGG TGATGTGTGA CAATCGTTTG 
AGCAACCGCA CCATTCCTCA GGGTTCTGTT TACAAGGTGG CTACGGATGA CGGCACCGTG 
CCCATCCAGA GTTACACTTT CGATTTTTTT AACTACGCGG GAATCCACCG GAGTGTACAC 
TTGTACACGA CCCCGCTGCT GCACATAAGC GATTTGGAGG TCACCACCCA ACTAACTAAA 
GAAGGATTGG GCCGCATAGA CTACCGCGTG TGGTTGGATG CTAGCAAGGA GGGCCTCCAA 
ATCCAGCCCA TCCAACTTCG GGTGCAACTA AGGGACAAAG ATGGTCATGT GGCCGCCCAG 
CAGATCAACA AGGCGGTCTA CCATGGTACT CTGCTGGTGC CAAATGCAAC TCCCTGGTGG 
CCCTACCTAA TGCATTCCGA TCCCGGCTAC CTGTATAATC TTCAATTCGA GCTGTTTGTG 
GCCAGCAATG AGAAGGAGTT GGAATCTCTG CAGGATACCT ACCGCCTCCC AGTGGGCATA 
CGTAGCTTAA GTTGGGACAA CGACAGCCTG CTGCTGAATG GAAAACCCCT CTATCTGCGG 
GGATTTGGAC GGCACGAGGA CTCCGATATC CGCGGGAAGG GATTGGATAA TGCGCTTCTT 
GCTAGAGATT TTAACCTGCT GAAGTGGACT GGAGCCAATG CATATCGCAC CTCTCACTAT 
CCTTATTCCG AAGAGTCAAT GCAGTTTGCC GATCAGCATG GTATTATGAT TATTGACGAA 
TGCCCTGCTG TCAATATAGA TATCTTCGAG CCGCAGCTAC TGGAGAATCA CATGTCCTCG 
CTGGAGCAAC TGATCCACCG GGACAGAAAC CATCCAAGTG TTGTTGCATG GTCGGTAGCC 
AACGAGCCGA GATCGAACAA GCAGGGAGCC CTTAAATACT TTGAATTCCT GGTAAACTAT 
GTAAGAGAAA TAGCTCACGG ACGACCTCTA ACCGCGGCTA TAAATGCCAA CTCTTCCAGT 
TGCCATTTGG CGCAGTTTCT GGACATCGTG GGTTTCAATC GCTATAACTC TTGGTATCAG 
AACTCGGGCC GTACTGACAT GATACTTAAC CTGTTGACGA TAGAGGCCCA GAGTTGGCGG 
GATAGGTTCG GAAAGCCTGT CATCCAATTT GAGTACGGCG GCGACACTAT GGAGGGCATG 
CACTCACTTC CCGCCTTTAT TTGGTCGGAG GAATACCAGG TTGAGCTCTT CTCTCGCCAT 
TTCAAGGCTT TCGACGAGTT ACGCGGAAGA GGATGGTTTA TTGGAGAGTT TGTTTGGAAC 
TTCGCCGATT TTCGGACCGC GCAGACTATT ACCCGCGTGG GCGGCAACAA AAAGGGAGTC 
TTTACAAGGA ACCGACAACC CAAAGAAGTG GCTCACATTC TTAGGCGGCG GTACTTCGCA 
TTAGCCAAGG AGTTGGATAT GTTTGGGCTG CCTGAGGATC TCACTGTTTA CATTTCCAAG 
CATATCCATA ACGAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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betaGlu cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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