awd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	awd
Entrez Gene ID	43739
Full Name	abnormal wing discs
Synonyms	1084/08,Awd,BcDNA:GM19775,BcDNA:RH27794,CG2210,Dmel\CG2210,K-pn,Kpn,NDKB,NDPK,Nm23,Nm23/awd,anon-EST:Liang-2.27,anon-EST:Liang-2.28,anon-WO0172774.80,anon-WO0172774.82,clone 2.27,clone 2.28,e(shi)A,eshiA,l(3)L8700,l(3)j2A4
General protein information	Preferred Names abnormal wing discs Names CG2210 gene product from transcript CG2210-RD CG2210-PC CG2210-PD CG2210-PE Killer-of-prune NDP kinase abnormal wing disc abnormal wings disc awd-PC awd-PD awd-PE killer of prune nucleoside diphosphate kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100D2-100D2\|3-104.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057413.4	NP_476761.3	abnormal wing discs, isoform C
NM_001300696.1	NP_001287625.1	abnormal wing discs, isoform D
NM_001300695.1	NP_001287624.1	abnormal wing discs, isoform E

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC101905864	XP_005220712.1
Caenorhabditis elegans (roundworm)	ndk-1	NP_492761.1
Neurospora crassa	NCU04202	XP_961162.1
	LOC707717	XP_001096144.2
Mus musculus (house mouse)	Gm5566	XP_006505013.1
Drosophila melanogaster (fruit fly)	awd	NP_476761.2
Arabidopsis thaliana (thale cress)	NDPK1	NP_567346.1
Oryza sativa (rice)	Os10g0563700	NP_001065404.1
Homo sapiens (human)	NME1-NME2	NP_001018146.1
Pan troglodytes (chimpanzee)	LOC101059613	XP_003952370.1
Danio rerio (zebrafish)	nme2a	NP_956264.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007120	XP_308641.4
Magnaporthe oryzae (rice blast fungus)	MGG_08622	XP_003711050.1
Saccharomyces cerevisiae (baker's yeast)	YNK1	NP_012856.1
Kluyveromyces lactis	KLLA0D03696g	XP_453229.1
Eremothecium gossypii	AGOS_ABR096C	NP_983042.2
Schizosaccharomyces pombe (fission yeast)	ndk1	NP_592857.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Down-regulation of Shi activity enhances extracellular Awd Drosophila cell lines.
Title: Dynamin controls extracellular level of Awd/Nme1 metastasis suppressor protein.

Interaction between Nm23 and the tumor suppressor VHL
Title: Interaction between Nm23 and the tumor suppressor VHL.

Awd function is required before gamma-secretase mediated cleavage since over-expression of the constitutively active form of the Notch receptor in awd mutant follicle cells allows rescue of the signaling.
Title: Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23.

Data show that awd modulates the level and localization of adherens junction components via endocytosis, and is involved in regulating morphogenesis.
Title: The Drosophila metastasis suppressor gene Nm23 homolog, awd, regulates epithelial integrity during oogenesis.

Awd is a negative regulator of ovary border-cell migration. Down-regulation allows optimal spatial signaling via Pvr & JAK/STAT. Overexpression stalls migration & reverts the phenotype of overexpressing constitutive Pvr or dominant-negative dynamin.
Title: Awd, the homolog of metastasis suppressor gene Nm23, regulates Drosophila epithelial cell invasion.

The following awd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the awd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa22183	NM_057413.4 Latest version!	Drosophila melanogaster abnormal wing discs (awd), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa22183	NM_001300696.1 Latest version!	Drosophila melanogaster abnormal wing discs (awd), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa22184	NM_001300695.1 Latest version!	Drosophila melanogaster abnormal wing discs (awd), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

awd ( NM_057413.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476761.3 Drosophila melanogaster abnormal wing discs (awd), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa22183
Clone ID Related Accession (Same CDS sequence)	NM_057413.4 , NM_001300696.1
Accession Version	NM_057413.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	abnormal wing discs, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_057413.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCGGCA CAATTTTGGC ATTCTTTTCT GTAATCTCGG CGACAATGGC GGCTAACAAG 
GAGAGGACTT TCATCATGGT CAAGCCCGAT GGCGTCCAGC GCGGGCTCGT CGGCAAGATC 
ATCGAGCGCT TCGAGCAGAA GGGCTTCAAG CTGGTCGCCC TGAAGTTCAC CTGGGCCTCC 
AAGGAGCTGC TGGAGAAGCA CTACGCTGAT CTGTCCGCCC GCCCCTTCTT CCCCGGACTC 
GTGAACTACA TGAACTCCGG CCCCGTGGTG CCCATGGTGT GGGAGGGTCT GAATGTGGTC 
AAGACCGGTC GCCAGATGCT CGGCGCCACC AACCCCGCCG ACTCGCTGCC CGGCACCATC 
CGCGGTGACT TCTGCATTCA GGTCGGACGC AACATCATCC ACGGCTCCGA TGCCGTCGAG 
TCTGCCGAGA AGGAGATCGC CCTGTGGTTC AACGAAAAGG AGCTGGTCAC CTGGACCCCG 
GCCGCCAAGG ACTGGATCTA CGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476761.3
CDS	42..548
Translation	MLGTILAFFSVISATMAANKERTFIMVKPDGVQRGLVGKIIERFEQKGFKLVALKFTWASKELLEKHYADLSARPFFPGLVNYMNSGPVVPMVWEGLNVVKTGRQMLGATNPADSLPGTIRGDFCIQVGRNIIHGSDAVESAEKEIALWFNEKELVTWTPAAKDWIYE

Target ORF information:

RefSeq Version	NM_057413.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster abnormal wing discs (awd), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057413.4

ORF Insert Sequence:

ATGCTCGGCA CAATTTTGGC ATTCTTTTCT GTAATCTCGG CGACAATGGC GGCTAACAAG 
GAGAGGACTT TCATCATGGT CAAGCCCGAT GGCGTCCAGC GCGGGCTCGT CGGCAAGATC 
ATCGAGCGCT TCGAGCAGAA GGGCTTCAAG CTGGTCGCCC TGAAGTTCAC CTGGGCCTCC 
AAGGAGCTGC TGGAGAAGCA CTACGCTGAT CTGTCCGCCC GCCCCTTCTT CCCCGGACTC 
GTGAACTACA TGAACTCCGG CCCCGTGGTG CCCATGGTGT GGGAGGGTCT GAATGTGGTC 
AAGACCGGTC GCCAGATGCT CGGCGCCACC AACCCCGCCG ACTCGCTGCC CGGCACCATC 
CGCGGTGACT TCTGCATTCA GGTCGGACGC AACATCATCC ACGGCTCCGA TGCCGTCGAG 
TCTGCCGAGA AGGAGATCGC CCTGTGGTTC AACGAAAAGG AGCTGGTCAC CTGGACCCCG 
GCCGCCAAGG ACTGGATCTA CGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

awd ( NM_001300696.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287625.1 Drosophila melanogaster abnormal wing discs (awd), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa22183
Clone ID Related Accession (Same CDS sequence)	NM_057413.4 , NM_001300696.1
Accession Version	NM_001300696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	abnormal wing discs, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCGGCA CAATTTTGGC ATTCTTTTCT GTAATCTCGG CGACAATGGC GGCTAACAAG 
GAGAGGACTT TCATCATGGT CAAGCCCGAT GGCGTCCAGC GCGGGCTCGT CGGCAAGATC 
ATCGAGCGCT TCGAGCAGAA GGGCTTCAAG CTGGTCGCCC TGAAGTTCAC CTGGGCCTCC 
AAGGAGCTGC TGGAGAAGCA CTACGCTGAT CTGTCCGCCC GCCCCTTCTT CCCCGGACTC 
GTGAACTACA TGAACTCCGG CCCCGTGGTG CCCATGGTGT GGGAGGGTCT GAATGTGGTC 
AAGACCGGTC GCCAGATGCT CGGCGCCACC AACCCCGCCG ACTCGCTGCC CGGCACCATC 
CGCGGTGACT TCTGCATTCA GGTCGGACGC AACATCATCC ACGGCTCCGA TGCCGTCGAG 
TCTGCCGAGA AGGAGATCGC CCTGTGGTTC AACGAAAAGG AGCTGGTCAC CTGGACCCCG 
GCCGCCAAGG ACTGGATCTA CGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287625.1
CDS	135..641
Translation	MLGTILAFFSVISATMAANKERTFIMVKPDGVQRGLVGKIIERFEQKGFKLVALKFTWASKELLEKHYADLSARPFFPGLVNYMNSGPVVPMVWEGLNVVKTGRQMLGATNPADSLPGTIRGDFCIQVGRNIIHGSDAVESAEKEIALWFNEKELVTWTPAAKDWIYE

Target ORF information:

RefSeq Version	NM_001300696.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster abnormal wing discs (awd), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300696.1

ORF Insert Sequence:

ATGCTCGGCA CAATTTTGGC ATTCTTTTCT GTAATCTCGG CGACAATGGC GGCTAACAAG 
GAGAGGACTT TCATCATGGT CAAGCCCGAT GGCGTCCAGC GCGGGCTCGT CGGCAAGATC 
ATCGAGCGCT TCGAGCAGAA GGGCTTCAAG CTGGTCGCCC TGAAGTTCAC CTGGGCCTCC 
AAGGAGCTGC TGGAGAAGCA CTACGCTGAT CTGTCCGCCC GCCCCTTCTT CCCCGGACTC 
GTGAACTACA TGAACTCCGG CCCCGTGGTG CCCATGGTGT GGGAGGGTCT GAATGTGGTC 
AAGACCGGTC GCCAGATGCT CGGCGCCACC AACCCCGCCG ACTCGCTGCC CGGCACCATC 
CGCGGTGACT TCTGCATTCA GGTCGGACGC AACATCATCC ACGGCTCCGA TGCCGTCGAG 
TCTGCCGAGA AGGAGATCGC CCTGTGGTTC AACGAAAAGG AGCTGGTCAC CTGGACCCCG 
GCCGCCAAGG ACTGGATCTA CGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

awd ( NM_001300695.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287624.1 Drosophila melanogaster abnormal wing discs (awd), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa22184
Clone ID Related Accession (Same CDS sequence)	NM_001300695.1
Accession Version	NM_001300695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	abnormal wing discs, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCTA ACAAGGAGAG GACTTTCATC ATGGTCAAGC CCGATGGCGT CCAGCGCGGG 
CTCGTCGGCA AGATCATCGA GCGCTTCGAG CAGAAGGGCT TCAAGCTGGT CGCCCTGAAG 
TTCACCTGGG CCTCCAAGGA GCTGCTGGAG AAGCACTACG CTGATCTGTC CGCCCGCCCC 
TTCTTCCCCG GACTCGTGAA CTACATGAAC TCCGGCCCCG TGGTGCCCAT GGTGTGGGAG 
GGTCTGAATG TGGTCAAGAC CGGTCGCCAG ATGCTCGGCG CCACCAACCC CGCCGACTCG 
CTGCCCGGCA CCATCCGCGG TGACTTCTGC ATTCAGGTCG GACGCAACAT CATCCACGGC 
TCCGATGCCG TCGAGTCTGC CGAGAAGGAG ATCGCCCTGT GGTTCAACGA AAAGGAGCTG 
GTCACCTGGA CCCCGGCCGC CAAGGACTGG ATCTACGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287624.1
CDS	31..492
Translation	MAANKERTFIMVKPDGVQRGLVGKIIERFEQKGFKLVALKFTWASKELLEKHYADLSARPFFPGLVNYMNSGPVVPMVWEGLNVVKTGRQMLGATNPADSLPGTIRGDFCIQVGRNIIHGSDAVESAEKEIALWFNEKELVTWTPAAKDWIYE

Target ORF information:

RefSeq Version	NM_001300695.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster abnormal wing discs (awd), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300695.1

ORF Insert Sequence:

ATGGCGGCTA ACAAGGAGAG GACTTTCATC ATGGTCAAGC CCGATGGCGT CCAGCGCGGG 
CTCGTCGGCA AGATCATCGA GCGCTTCGAG CAGAAGGGCT TCAAGCTGGT CGCCCTGAAG 
TTCACCTGGG CCTCCAAGGA GCTGCTGGAG AAGCACTACG CTGATCTGTC CGCCCGCCCC 
TTCTTCCCCG GACTCGTGAA CTACATGAAC TCCGGCCCCG TGGTGCCCAT GGTGTGGGAG 
GGTCTGAATG TGGTCAAGAC CGGTCGCCAG ATGCTCGGCG CCACCAACCC CGCCGACTCG 
CTGCCCGGCA CCATCCGCGG TGACTTCTGC ATTCAGGTCG GACGCAACAT CATCCACGGC 
TCCGATGCCG TCGAGTCTGC CGAGAAGGAG ATCGCCCTGT GGTTCAACGA AAAGGAGCTG 
GTCACCTGGA CCCCGGCCGC CAAGGACTGG ATCTACGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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awd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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