Gcn2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gcn2
Entrez Gene ID	43709
Full Name	CG1609 gene product from transcript CG1609-RA
Synonyms	CG1609,DGCN2,Dmel\CG1609,GCN2,Gcn-2,dGCN2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100C3-100C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276212.1	NP_001263141.1	Gcn2, isoform B
NM_057882.4	NP_477230.1	Gcn2, isoform A
NM_001276213.1	NP_001263142.1	Gcn2, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EIF2AK4	XP_001140245.1
	EIF2AK4	XP_002804763.1
Gallus gallus (chicken)	EIF2AK4	XP_004941777.1
Eremothecium gossypii	AGOS_AAL083W	NP_982459.1
Rattus norvegicus (Norway rat)	Eif2ak4	NP_001099214.2
Danio rerio (zebrafish)	eif2ak4	XP_002667149.3
Drosophila melanogaster (fruit fly)	Gcn2	NP_001263141.1
Saccharomyces cerevisiae (baker's yeast)	GCN2	NP_010569.3
Kluyveromyces lactis	KLLA0C10802g	XP_452681.1
Neurospora crassa	NCU01187	XP_961553.1
Xenopus tropicalis (tropical clawed frog)	eif2ak4	NP_001243855.1
Homo sapiens (human)	EIF2AK4	NP_001013725.2
Canis lupus familiaris (dog)	EIF2AK4	XP_535431.3
Bos taurus (cattle)	EIF2AK4	XP_002690831.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012368	XP_320188.4
Caenorhabditis elegans (roundworm)	gcn-2	NP_001254370.1
Schizosaccharomyces pombe (fission yeast)	gcn2	NP_595991.2
Mus musculus (house mouse)	Eif2ak4	NP_038747.2
Magnaporthe oryzae (rice blast fungus)	MGG_08643	XP_003711074.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GCN2 and ATF4 are important regulators of 4E-BP transcription during normal drosophila development and aging.
Title: 4E-BP is a target of the GCN2-ATF4 pathway during Drosophila development and aging.

this study have characterised the first example of an invertebrate eIF2a phosphatase PPP1R15 (CG3825) and demonstrated that during development it primarily antagonises the function of GCN2.
Title: Coordinate regulation of eIF2? phosphorylation by PPP1R15 and GCN2 is required during Drosophila development.

The following Gcn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gcn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06757	NM_057882.4 Latest version!	Drosophila melanogaster Gcn2, transcript variant A (Gcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa06757	NM_001276212.1 Latest version!	Drosophila melanogaster Gcn2, transcript variant B (Gcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa07869	NM_001276213.1 Latest version!	Drosophila melanogaster Gcn2, transcript variant C (Gcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gcn2 ( NM_001276212.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263141.1 Drosophila melanogaster Gcn2, transcript variant B (Gcn2), mRNA.

Price & Availability↑

CloneID	OFa06757
Clone ID Related Accession (Same CDS sequence)	NM_001276212.1 , NM_057882.4
Accession Version	NM_001276212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gcn2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGATG AAAAGGCCAA GGAATCCTTC CGCGAGCGGC AAGCGCAGGA GTTGGAGGTT 
ATAAAGTCCA TCTTTGGCTG TGATGTGGAG GACCTAAGAC CCCAGGCGAA TCCTTCCCTG 
TGGAAGCCAA CGGACATACG GATACAACTT ACCCCACTTC GAGACTCCTC GAACGGACTG 
GAAACCTATG TGTGCACCAA GCTGCACGTC ACCTGTCCAT CAAAGTATCC AAAATTGCCC 
CCTAAGATAT CTCTGGAGGA GTCCAAAGGC ATGTCGGATC AGCTGCTGGA GGCCCTACGC 
AACCAATTGC AGGCTCAATC CCAAGAGCTA CGTGGCGAAG TGATGATTTA TGAATTGGCC 
CAGACCGTTC AGGCCTTTCT CCTCGAACAC AACAAACCAC CAAAGGGATC ATTCTACGAC 
CAGATGCTCC AGGATAAACA GAAGCGCGAC CAGGAGCTAC AGGACATACA GCGGCAGCGG 
GAGTCCCTGC AACGCCAGAC GCTCATCGAC GAGGTGGAGC GGCGCAAAGA GATGTTCAAA 
ACGGAGGCTA AGAGGCGCGG CGAACCAAGG CGCAGCATGA GCGAGTCTAA CCCACGGCAT 
CCTAGCTCGT CGGAGAGCTC CGAGAACTCC TCGCCCTACT ATCGTGGTCA CATCTACCCC 
TCCAAATGCC TGGATCACCG CAACACGGAG ACGCTCTACT TCCACAAGAT GGGCAGGCAG 
ATTCAGCGTG GATGCTGCGT TGGTCACTCC CAACGAGGTT GCATCGCCTA CACTGGCATA 
GATATGCACT GCGGCCAGTT GCTCTACATA ACAGAATGGA ACATCAAGTA CAGCCAACTG 
GAGCAGCCAT GCATCGGCGG AGGCAAGTGC CACTGGAGCA GCGAGTCAAA GTGCATGGGC 
AGTCATCGTG TGGACGAAGT GATGGCCTCC ATTGAAAAGC AGGTGTCCTC ACTGTCCCAA 
CTACAGCACA AAAACCTCGT CTCGTACGAG TGCGTACTGT GCATCAAGCG AAAGGAAGGT 
CTACTTGTGT ATTTGGTCCA AGACTTCCTC CTTGGCACCA GCGTGTTCAG CATCTCCTCC 
TCGCTTGGCT GGTGCATGGA CGGCGCACGA ATGGTTGCGC GCGGCGTGCT GGACGCCTTA 
GTATTTCTTC ACAACAAGGG TGTATCCCAT AGTCACCTGC TCGACACCAC TGTTTTTATG 
GACAACACAG GCAATGTGAG GGTATCAGAC TTTTCGCTCG TTCCCAATCT TCTAGAACTT 
CTGAGCGGAG CTGGACAAAG CAGCAGTTGC GGGGACTTAC CCGCGTTAGG TGCCCTGGTG 
GAGAGTTTGA TGCCCACTAA CAGTTACGAG ATGCGCGACT TTGTAGACAA GTGTAACTCG 
GACCGAACTC TTTCCGCATC AGAGTTATTG GAGCATCCAT TCTTACGCTT TTACGTCGAC 
AATGGCCAGC AGCAGGTGAT GCCATTACCA CAGCAGCAAC ATCCTAACAC CGTCCAGCGC 
ACTGGATCCG CCATGCCGTA CCAGATACCG ACACTGGCCC TAAGCCAATC CCGCCTGCGA 
ACGGAATTCG AGGTGCTCAT GTACTTGGGA AAGGGTGCCT TTGGTGACGT GCTAAAGGTG 
CGCAACATTT TAGACAACCG AGAGTACGCC ATCAAGCGCA TACCATTGCC CGCGAGGAGT 
CGCCAGCTGT ACAAAAAGAT GACGCGCGAG GTGGAGCTTC TTTCGCGGCT GAATCATGAG 
AATGTAGTTC GATACTTTAA CAGTTGGATA GAAAGTGTAG ATGACGCAGA TGCCGCTGAG 
ATGGATAAGC TGCTGGGCGG TGAGTGGTCC CAGAGTCAGC AGGACCTTAG CGTAAAACCT 
GCCAAGTCAC CACAGCTGGG GCCCACTTTA GAGGAGGACG AGGATGAAGA GGACAGCTCA 
TCCAGCATGT GGAACGGTTA CATACCCAAC ATGGAGGACT CTGATTCGGA TGGCATTGAA 
TTTGTGGACT CCAACGGCAA GGTGGCTGTC TACGACGACG AAGAGCAGGA GGATTCCACC 
AGAGGCAAGA CCAATTCGCC AAAACCTTTG ATGCAGGTCA TGTACATCCA GATGGAGTTC 
TGTGAGAAAT GCACATTGCG CACCGCCATC GATGACAATC TCTTCAATGA CACGGATCGT 
CTGTGGCGCT TATTTCGGGA GATTGCCGAG GGTCTCGCGC ATATTCACCA GCAAGGCATT 
ATCCATCGGG ACCTAAAGCC CGTTAACATC TTCTTGGACT CGCACGATCA GATTAAGATC 
GGTGACTTTG GTCTGGCCAC CACCAGTTTT CTGGCTCTCC AAGCCCACGA CGCAGCGCCT 
GCGCCTGTTA ACCAGATAAC CAGTGCGGAA GACGGCACTG GTACTGGTAA GGTCGGCACC 
ACTCTATACG TGGCTCCCGA ATTGACAGGA AATGCTTCCA AGTCTGTGTA CAACCAGAAG 
GTTGATATGT ACACCCTGGG AATCATTCTT TTCGAGATGT GCCAACCGCC GTTCGACACA 
AGCATGGAGC GGGCTCAGAC CATTATGGCT CTCAGGAATG TTTCCATTAA TATACCGGAT 
GCTATGCTTA AGGATCCCAA GTATGAGAAG ACGGTGAAGA TGCTTCAGTG GCTTCTCAAC 
CACGATCCCG CCCAGAGACC TACTGCCGAG GAGCTGCTCA TCTCAGACCT GGTTCCACCA 
GCCCAGTTGG AGGCCAATGA GCTCCAAGAA ATGCTGCGTC ACGCCCTCGC TAATCCGCAG 
AGCAAGGCCT ACAAGAACCT AGTTGCCCGA TGTCTTCAGC AGGAGAGCGA CGAGGTGCTG 
GAACACACCT ACCACTTGGG AAGCAGTCGA GCTATGAAAT CCTGGAACTC GGCAATTATA 
ATCGATGATA TAGTATCCCT GAACCCAGTG ATTGAGTTTG TTAAGGCTAA GGTCGTGAAT 
TTATTCCGCA AGCATGGAGC TATAGAGGTG GACTCACCGC TCTTATCCCC ACTTTCCGCA 
AGGAACAGCA CTGCGAATGC GAACGCCAAT GCCGTACACC TTATGACCCA CTCGGGATGT 
GTGGTGGTGC TACCCTGTGA CCTGCGGACT CAGTTTGCGC GTCACGTTAC CATGAACAGC 
GTGAATCTCA TCCGGCGCTA CTGTGTGGAC CGTGTCTATC GGGAGGAGCG AGTGTTTAAT 
TTTCATCCCA AGCAAAGCTA CGACTGCTCT TTCGATATAA TTGCACCCAC AACTGGAAGT 
CATTTAGTTG ATGCCGAGCT GCTGTCGTTG GCTTTTGAAA TCACCAGTGA ACTGCCCCGA 
TTACGCGAAA AGAATTTGGC GATCCGCATG AACCACACTA ATCTCTTGAG GGCCATCCTC 
ATCTTCTGCA ATGTACCAAA GGCTCAGTAT GGAGCACTCT TCGAGGGCAC CATGGACTTC 
ATCGAGTCGC GCATCTCACG CTTCCAATTT CACTCGAGTA TTACCGGGAT TATGGAAAAG 
TCGCGAACAT CTGCTCAAAC TCTGATGGAC ATGTTGTTAG CTAACTTTCT GCTGACTGGT 
TCCAGAAGCA CTGTGGACGA TTCCGCGCTG AAATCTTTGA TGCGTGGAAA GGGAGAAGCA 
GCTTCACTGG CAAGGGGAGC ATTGCGGGAA CTTGAAACAG TTGTGGGACT GGCTTACAGT 
CTAGGCGTAA AGTGTCCTAT ACACATATGG GCTGGCTTGC CCATCAGCTT CGATCGTGCC 
AGCAACGGTG GAATTGTGTG GCAGATGACC GCTGACCTGA AGCCCAATCG CTCTGGCCAT 
CCTTCGGTTC TGGCGATTGG GGAGAGATAC GACTCTATGC TGCATGAATT CCAGAAGCAG 
GCGCAGAAGT TTAATCCCGC CATGCCAGCA CGTGGAGTTC TTAGTGGAGC GGGCTTGACG 
TTCTCCCTAG ACAAGCTGGT AGCTGCTGTT GGTGTGGAAT ACGCTAAGGA TTGTCGGGCG 
ATAGATGTGG GCATTTGCGT GTGTGGAACC CGTCCACCGC TAAAGGATGT GACCTATATC 
ATGCGTCTGC TGTGGTCGGT TGGCATCCGA TGTGGAATTG TGGAGGCCGC CAGCGAATTG 
GGAGATGAAG CACAGGATCT TGCCCGCCTT GGGGCGTTAC ATGTCATCCT GGTGGCGGAA 
AATGGCTCAT TGAGAGTGAG ATCTTTCGAG AGGGAACGAT TCCAGGAGCG TCACCTAACA 
AGAACCGAAC TGGTGGAGTT CATCCAGAAG ATGCTGCGAA GTGATGGACT CAATGGGACC 
ACCGTGGACA ACTTCAGCCA GTTGTCAGCC TTGGGTAGTG GTGACAACAG AAGCAGTGGT 
GGAAAGGAAA GAGAACGCGG CGAGAATGGT CTGAGCACCT CCGCATCGAA TGCCACCATC 
AAGAACAACT ATAGCCAGTT GCCGAATCTT CAGGTGACGT TTCTCACCCA CGACAAGCCA 
ACGGCGAACT ACAAAAGGCG GCTAGAGAAC CAGGTGGCGC AGCAGATGAG CTCAACGCTA 
TCGCAGTTCC TCAAGAAGGA GACCTTTGTG GTCCTGGTGG TGGAGTTGCC GCCTGCTGTT 
GTAAATGCCA TTGTGGGGGC CATTAATCCA CGGGAAATAC GCAAGCGAGA GACTGAACCG 
GAAATCAACT ATGTGATCGA GCGATTTTCG AAATACAAGC GCTACATCTC CGAGATAAAC 
GAAGAGGTTG TCGACTATCT GAGCGATGCC AAGACTCCCA TTGTGGCCTT GTACAGCATA 
TCCGATTCCT ACTACCGCGT CATCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263141.1
CDS	332..5101
Translation	MADEKAKESFRERQAQELEVIKSIFGCDVEDLRPQANPSLWKPTDIRIQLTPLRDSSNGLETYVCTKLHVTCPSKYPKLPPKISLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKPPKGSFYDQMLQDKQKRDQELQDIQRQRESLQRQTLIDEVERRKEMFKTEAKRRGEPRRSMSESNPRHPSSSESSENSSPYYRGHIYPSKCLDHRNTETLYFHKMGRQIQRGCCVGHSQRGCIAYTGIDMHCGQLLYITEWNIKYSQLEQPCIGGGKCHWSSESKCMGSHRVDEVMASIEKQVSSLSQLQHKNLVSYECVLCIKRKEGLLVYLVQDFLLGTSVFSISSSLGWCMDGARMVARGVLDALVFLHNKGVSHSHLLDTTVFMDNTGNVRVSDFSLVPNLLELLSGAGQSSSCGDLPALGALVESLMPTNSYEMRDFVDKCNSDRTLSASELLEHPFLRFYVDNGQQQVMPLPQQQHPNTVQRTGSAMPYQIPTLALSQSRLRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPARSRQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDADAAEMDKLLGGEWSQSQQDLSVKPAKSPQLGPTLEEDEDEEDSSSSMWNGYIPNMEDSDSDGIEFVDSNGKVAVYDDEEQEDSTRGKTNSPKPLMQVMYIQMEFCEKCTLRTAIDDNLFNDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDFGLATTSFLALQAHDAAPAPVNQITSAEDGTGTGKVGTTLYVAPELTGNASKSVYNQKVDMYTLGIILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKTVKMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALANPQSKAYKNLVARCLQQESDEVLEHTYHLGSSRAMKSWNSAIIIDDIVSLNPVIEFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSTANANANAVHLMTHSGCVVVLPCDLRTQFARHVTMNSVNLIRRYCVDRVYREERVFNFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFEITSELPRLREKNLAIRMNHTNLLRAILIFCNVPKAQYGALFEGTMDFIESRISRFQFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDDSALKSLMRGKGEAASLARGALRELETVVGLAYSLGVKCPIHIWAGLPISFDRASNGGIVWQMTADLKPNRSGHPSVLAIGERYDSMLHEFQKQAQKFNPAMPARGVLSGAGLTFSLDKLVAAVGVEYAKDCRAIDVGICVCGTRPPLKDVTYIMRLLWSVGIRCGIVEAASELGDEAQDLARLGALHVILVAENGSLRVRSFERERFQERHLTRTELVEFIQKMLRSDGLNGTTVDNFSQLSALGSGDNRSSGGKERERGENGLSTSASNATIKNNYSQLPNLQVTFLTHDKPTANYKRRLENQVAQQMSSTLSQFLKKETFVVLVVELPPAVVNAIVGAINPREIRKRETEPEINYVIERFSKYKRYISEINEEVVDYLSDAKTPIVALYSISDSYYRVII

Target ORF information:

RefSeq Version	NM_001276212.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gcn2, transcript variant B (Gcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276212.1

ORF Insert Sequence:

ATGGCGGATG AAAAGGCCAA GGAATCCTTC CGCGAGCGGC AAGCGCAGGA GTTGGAGGTT 
ATAAAGTCCA TCTTTGGCTG TGATGTGGAG GACCTAAGAC CCCAGGCGAA TCCTTCCCTG 
TGGAAGCCAA CGGACATACG GATACAACTT ACCCCACTTC GAGACTCCTC GAACGGACTG 
GAAACCTATG TGTGCACCAA GCTGCACGTC ACCTGTCCAT CAAAGTATCC AAAATTGCCC 
CCTAAGATAT CTCTGGAGGA GTCCAAAGGC ATGTCGGATC AGCTGCTGGA GGCCCTACGC 
AACCAATTGC AGGCTCAATC CCAAGAGCTA CGTGGCGAAG TGATGATTTA TGAATTGGCC 
CAGACCGTTC AGGCCTTTCT CCTCGAACAC AACAAACCAC CAAAGGGATC ATTCTACGAC 
CAGATGCTCC AGGATAAACA GAAGCGCGAC CAGGAGCTAC AGGACATACA GCGGCAGCGG 
GAGTCCCTGC AACGCCAGAC GCTCATCGAC GAGGTGGAGC GGCGCAAAGA GATGTTCAAA 
ACGGAGGCTA AGAGGCGCGG CGAACCAAGG CGCAGCATGA GCGAGTCTAA CCCACGGCAT 
CCTAGCTCGT CGGAGAGCTC CGAGAACTCC TCGCCCTACT ATCGTGGTCA CATCTACCCC 
TCCAAATGCC TGGATCACCG CAACACGGAG ACGCTCTACT TCCACAAGAT GGGCAGGCAG 
ATTCAGCGTG GATGCTGCGT TGGTCACTCC CAACGAGGTT GCATCGCCTA CACTGGCATA 
GATATGCACT GCGGCCAGTT GCTCTACATA ACAGAATGGA ACATCAAGTA CAGCCAACTG 
GAGCAGCCAT GCATCGGCGG AGGCAAGTGC CACTGGAGCA GCGAGTCAAA GTGCATGGGC 
AGTCATCGTG TGGACGAAGT GATGGCCTCC ATTGAAAAGC AGGTGTCCTC ACTGTCCCAA 
CTACAGCACA AAAACCTCGT CTCGTACGAG TGCGTACTGT GCATCAAGCG AAAGGAAGGT 
CTACTTGTGT ATTTGGTCCA AGACTTCCTC CTTGGCACCA GCGTGTTCAG CATCTCCTCC 
TCGCTTGGCT GGTGCATGGA CGGCGCACGA ATGGTTGCGC GCGGCGTGCT GGACGCCTTA 
GTATTTCTTC ACAACAAGGG TGTATCCCAT AGTCACCTGC TCGACACCAC TGTTTTTATG 
GACAACACAG GCAATGTGAG GGTATCAGAC TTTTCGCTCG TTCCCAATCT TCTAGAACTT 
CTGAGCGGAG CTGGACAAAG CAGCAGTTGC GGGGACTTAC CCGCGTTAGG TGCCCTGGTG 
GAGAGTTTGA TGCCCACTAA CAGTTACGAG ATGCGCGACT TTGTAGACAA GTGTAACTCG 
GACCGAACTC TTTCCGCATC AGAGTTATTG GAGCATCCAT TCTTACGCTT TTACGTCGAC 
AATGGCCAGC AGCAGGTGAT GCCATTACCA CAGCAGCAAC ATCCTAACAC CGTCCAGCGC 
ACTGGATCCG CCATGCCGTA CCAGATACCG ACACTGGCCC TAAGCCAATC CCGCCTGCGA 
ACGGAATTCG AGGTGCTCAT GTACTTGGGA AAGGGTGCCT TTGGTGACGT GCTAAAGGTG 
CGCAACATTT TAGACAACCG AGAGTACGCC ATCAAGCGCA TACCATTGCC CGCGAGGAGT 
CGCCAGCTGT ACAAAAAGAT GACGCGCGAG GTGGAGCTTC TTTCGCGGCT GAATCATGAG 
AATGTAGTTC GATACTTTAA CAGTTGGATA GAAAGTGTAG ATGACGCAGA TGCCGCTGAG 
ATGGATAAGC TGCTGGGCGG TGAGTGGTCC CAGAGTCAGC AGGACCTTAG CGTAAAACCT 
GCCAAGTCAC CACAGCTGGG GCCCACTTTA GAGGAGGACG AGGATGAAGA GGACAGCTCA 
TCCAGCATGT GGAACGGTTA CATACCCAAC ATGGAGGACT CTGATTCGGA TGGCATTGAA 
TTTGTGGACT CCAACGGCAA GGTGGCTGTC TACGACGACG AAGAGCAGGA GGATTCCACC 
AGAGGCAAGA CCAATTCGCC AAAACCTTTG ATGCAGGTCA TGTACATCCA GATGGAGTTC 
TGTGAGAAAT GCACATTGCG CACCGCCATC GATGACAATC TCTTCAATGA CACGGATCGT 
CTGTGGCGCT TATTTCGGGA GATTGCCGAG GGTCTCGCGC ATATTCACCA GCAAGGCATT 
ATCCATCGGG ACCTAAAGCC CGTTAACATC TTCTTGGACT CGCACGATCA GATTAAGATC 
GGTGACTTTG GTCTGGCCAC CACCAGTTTT CTGGCTCTCC AAGCCCACGA CGCAGCGCCT 
GCGCCTGTTA ACCAGATAAC CAGTGCGGAA GACGGCACTG GTACTGGTAA GGTCGGCACC 
ACTCTATACG TGGCTCCCGA ATTGACAGGA AATGCTTCCA AGTCTGTGTA CAACCAGAAG 
GTTGATATGT ACACCCTGGG AATCATTCTT TTCGAGATGT GCCAACCGCC GTTCGACACA 
AGCATGGAGC GGGCTCAGAC CATTATGGCT CTCAGGAATG TTTCCATTAA TATACCGGAT 
GCTATGCTTA AGGATCCCAA GTATGAGAAG ACGGTGAAGA TGCTTCAGTG GCTTCTCAAC 
CACGATCCCG CCCAGAGACC TACTGCCGAG GAGCTGCTCA TCTCAGACCT GGTTCCACCA 
GCCCAGTTGG AGGCCAATGA GCTCCAAGAA ATGCTGCGTC ACGCCCTCGC TAATCCGCAG 
AGCAAGGCCT ACAAGAACCT AGTTGCCCGA TGTCTTCAGC AGGAGAGCGA CGAGGTGCTG 
GAACACACCT ACCACTTGGG AAGCAGTCGA GCTATGAAAT CCTGGAACTC GGCAATTATA 
ATCGATGATA TAGTATCCCT GAACCCAGTG ATTGAGTTTG TTAAGGCTAA GGTCGTGAAT 
TTATTCCGCA AGCATGGAGC TATAGAGGTG GACTCACCGC TCTTATCCCC ACTTTCCGCA 
AGGAACAGCA CTGCGAATGC GAACGCCAAT GCCGTACACC TTATGACCCA CTCGGGATGT 
GTGGTGGTGC TACCCTGTGA CCTGCGGACT CAGTTTGCGC GTCACGTTAC CATGAACAGC 
GTGAATCTCA TCCGGCGCTA CTGTGTGGAC CGTGTCTATC GGGAGGAGCG AGTGTTTAAT 
TTTCATCCCA AGCAAAGCTA CGACTGCTCT TTCGATATAA TTGCACCCAC AACTGGAAGT 
CATTTAGTTG ATGCCGAGCT GCTGTCGTTG GCTTTTGAAA TCACCAGTGA ACTGCCCCGA 
TTACGCGAAA AGAATTTGGC GATCCGCATG AACCACACTA ATCTCTTGAG GGCCATCCTC 
ATCTTCTGCA ATGTACCAAA GGCTCAGTAT GGAGCACTCT TCGAGGGCAC CATGGACTTC 
ATCGAGTCGC GCATCTCACG CTTCCAATTT CACTCGAGTA TTACCGGGAT TATGGAAAAG 
TCGCGAACAT CTGCTCAAAC TCTGATGGAC ATGTTGTTAG CTAACTTTCT GCTGACTGGT 
TCCAGAAGCA CTGTGGACGA TTCCGCGCTG AAATCTTTGA TGCGTGGAAA GGGAGAAGCA 
GCTTCACTGG CAAGGGGAGC ATTGCGGGAA CTTGAAACAG TTGTGGGACT GGCTTACAGT 
CTAGGCGTAA AGTGTCCTAT ACACATATGG GCTGGCTTGC CCATCAGCTT CGATCGTGCC 
AGCAACGGTG GAATTGTGTG GCAGATGACC GCTGACCTGA AGCCCAATCG CTCTGGCCAT 
CCTTCGGTTC TGGCGATTGG GGAGAGATAC GACTCTATGC TGCATGAATT CCAGAAGCAG 
GCGCAGAAGT TTAATCCCGC CATGCCAGCA CGTGGAGTTC TTAGTGGAGC GGGCTTGACG 
TTCTCCCTAG ACAAGCTGGT AGCTGCTGTT GGTGTGGAAT ACGCTAAGGA TTGTCGGGCG 
ATAGATGTGG GCATTTGCGT GTGTGGAACC CGTCCACCGC TAAAGGATGT GACCTATATC 
ATGCGTCTGC TGTGGTCGGT TGGCATCCGA TGTGGAATTG TGGAGGCCGC CAGCGAATTG 
GGAGATGAAG CACAGGATCT TGCCCGCCTT GGGGCGTTAC ATGTCATCCT GGTGGCGGAA 
AATGGCTCAT TGAGAGTGAG ATCTTTCGAG AGGGAACGAT TCCAGGAGCG TCACCTAACA 
AGAACCGAAC TGGTGGAGTT CATCCAGAAG ATGCTGCGAA GTGATGGACT CAATGGGACC 
ACCGTGGACA ACTTCAGCCA GTTGTCAGCC TTGGGTAGTG GTGACAACAG AAGCAGTGGT 
GGAAAGGAAA GAGAACGCGG CGAGAATGGT CTGAGCACCT CCGCATCGAA TGCCACCATC 
AAGAACAACT ATAGCCAGTT GCCGAATCTT CAGGTGACGT TTCTCACCCA CGACAAGCCA 
ACGGCGAACT ACAAAAGGCG GCTAGAGAAC CAGGTGGCGC AGCAGATGAG CTCAACGCTA 
TCGCAGTTCC TCAAGAAGGA GACCTTTGTG GTCCTGGTGG TGGAGTTGCC GCCTGCTGTT 
GTAAATGCCA TTGTGGGGGC CATTAATCCA CGGGAAATAC GCAAGCGAGA GACTGAACCG 
GAAATCAACT ATGTGATCGA GCGATTTTCG AAATACAAGC GCTACATCTC CGAGATAAAC 
GAAGAGGTTG TCGACTATCT GAGCGATGCC AAGACTCCCA TTGTGGCCTT GTACAGCATA 
TCCGATTCCT ACTACCGCGT CATCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gcn2 ( NM_057882.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477230.1 Drosophila melanogaster Gcn2, transcript variant A (Gcn2), mRNA.

Price & Availability↑

CloneID	OFa06757
Clone ID Related Accession (Same CDS sequence)	NM_001276212.1 , NM_057882.4
Accession Version	NM_057882.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gcn2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057882.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGATG AAAAGGCCAA GGAATCCTTC CGCGAGCGGC AAGCGCAGGA GTTGGAGGTT 
ATAAAGTCCA TCTTTGGCTG TGATGTGGAG GACCTAAGAC CCCAGGCGAA TCCTTCCCTG 
TGGAAGCCAA CGGACATACG GATACAACTT ACCCCACTTC GAGACTCCTC GAACGGACTG 
GAAACCTATG TGTGCACCAA GCTGCACGTC ACCTGTCCAT CAAAGTATCC AAAATTGCCC 
CCTAAGATAT CTCTGGAGGA GTCCAAAGGC ATGTCGGATC AGCTGCTGGA GGCCCTACGC 
AACCAATTGC AGGCTCAATC CCAAGAGCTA CGTGGCGAAG TGATGATTTA TGAATTGGCC 
CAGACCGTTC AGGCCTTTCT CCTCGAACAC AACAAACCAC CAAAGGGATC ATTCTACGAC 
CAGATGCTCC AGGATAAACA GAAGCGCGAC CAGGAGCTAC AGGACATACA GCGGCAGCGG 
GAGTCCCTGC AACGCCAGAC GCTCATCGAC GAGGTGGAGC GGCGCAAAGA GATGTTCAAA 
ACGGAGGCTA AGAGGCGCGG CGAACCAAGG CGCAGCATGA GCGAGTCTAA CCCACGGCAT 
CCTAGCTCGT CGGAGAGCTC CGAGAACTCC TCGCCCTACT ATCGTGGTCA CATCTACCCC 
TCCAAATGCC TGGATCACCG CAACACGGAG ACGCTCTACT TCCACAAGAT GGGCAGGCAG 
ATTCAGCGTG GATGCTGCGT TGGTCACTCC CAACGAGGTT GCATCGCCTA CACTGGCATA 
GATATGCACT GCGGCCAGTT GCTCTACATA ACAGAATGGA ACATCAAGTA CAGCCAACTG 
GAGCAGCCAT GCATCGGCGG AGGCAAGTGC CACTGGAGCA GCGAGTCAAA GTGCATGGGC 
AGTCATCGTG TGGACGAAGT GATGGCCTCC ATTGAAAAGC AGGTGTCCTC ACTGTCCCAA 
CTACAGCACA AAAACCTCGT CTCGTACGAG TGCGTACTGT GCATCAAGCG AAAGGAAGGT 
CTACTTGTGT ATTTGGTCCA AGACTTCCTC CTTGGCACCA GCGTGTTCAG CATCTCCTCC 
TCGCTTGGCT GGTGCATGGA CGGCGCACGA ATGGTTGCGC GCGGCGTGCT GGACGCCTTA 
GTATTTCTTC ACAACAAGGG TGTATCCCAT AGTCACCTGC TCGACACCAC TGTTTTTATG 
GACAACACAG GCAATGTGAG GGTATCAGAC TTTTCGCTCG TTCCCAATCT TCTAGAACTT 
CTGAGCGGAG CTGGACAAAG CAGCAGTTGC GGGGACTTAC CCGCGTTAGG TGCCCTGGTG 
GAGAGTTTGA TGCCCACTAA CAGTTACGAG ATGCGCGACT TTGTAGACAA GTGTAACTCG 
GACCGAACTC TTTCCGCATC AGAGTTATTG GAGCATCCAT TCTTACGCTT TTACGTCGAC 
AATGGCCAGC AGCAGGTGAT GCCATTACCA CAGCAGCAAC ATCCTAACAC CGTCCAGCGC 
ACTGGATCCG CCATGCCGTA CCAGATACCG ACACTGGCCC TAAGCCAATC CCGCCTGCGA 
ACGGAATTCG AGGTGCTCAT GTACTTGGGA AAGGGTGCCT TTGGTGACGT GCTAAAGGTG 
CGCAACATTT TAGACAACCG AGAGTACGCC ATCAAGCGCA TACCATTGCC CGCGAGGAGT 
CGCCAGCTGT ACAAAAAGAT GACGCGCGAG GTGGAGCTTC TTTCGCGGCT GAATCATGAG 
AATGTAGTTC GATACTTTAA CAGTTGGATA GAAAGTGTAG ATGACGCAGA TGCCGCTGAG 
ATGGATAAGC TGCTGGGCGG TGAGTGGTCC CAGAGTCAGC AGGACCTTAG CGTAAAACCT 
GCCAAGTCAC CACAGCTGGG GCCCACTTTA GAGGAGGACG AGGATGAAGA GGACAGCTCA 
TCCAGCATGT GGAACGGTTA CATACCCAAC ATGGAGGACT CTGATTCGGA TGGCATTGAA 
TTTGTGGACT CCAACGGCAA GGTGGCTGTC TACGACGACG AAGAGCAGGA GGATTCCACC 
AGAGGCAAGA CCAATTCGCC AAAACCTTTG ATGCAGGTCA TGTACATCCA GATGGAGTTC 
TGTGAGAAAT GCACATTGCG CACCGCCATC GATGACAATC TCTTCAATGA CACGGATCGT 
CTGTGGCGCT TATTTCGGGA GATTGCCGAG GGTCTCGCGC ATATTCACCA GCAAGGCATT 
ATCCATCGGG ACCTAAAGCC CGTTAACATC TTCTTGGACT CGCACGATCA GATTAAGATC 
GGTGACTTTG GTCTGGCCAC CACCAGTTTT CTGGCTCTCC AAGCCCACGA CGCAGCGCCT 
GCGCCTGTTA ACCAGATAAC CAGTGCGGAA GACGGCACTG GTACTGGTAA GGTCGGCACC 
ACTCTATACG TGGCTCCCGA ATTGACAGGA AATGCTTCCA AGTCTGTGTA CAACCAGAAG 
GTTGATATGT ACACCCTGGG AATCATTCTT TTCGAGATGT GCCAACCGCC GTTCGACACA 
AGCATGGAGC GGGCTCAGAC CATTATGGCT CTCAGGAATG TTTCCATTAA TATACCGGAT 
GCTATGCTTA AGGATCCCAA GTATGAGAAG ACGGTGAAGA TGCTTCAGTG GCTTCTCAAC 
CACGATCCCG CCCAGAGACC TACTGCCGAG GAGCTGCTCA TCTCAGACCT GGTTCCACCA 
GCCCAGTTGG AGGCCAATGA GCTCCAAGAA ATGCTGCGTC ACGCCCTCGC TAATCCGCAG 
AGCAAGGCCT ACAAGAACCT AGTTGCCCGA TGTCTTCAGC AGGAGAGCGA CGAGGTGCTG 
GAACACACCT ACCACTTGGG AAGCAGTCGA GCTATGAAAT CCTGGAACTC GGCAATTATA 
ATCGATGATA TAGTATCCCT GAACCCAGTG ATTGAGTTTG TTAAGGCTAA GGTCGTGAAT 
TTATTCCGCA AGCATGGAGC TATAGAGGTG GACTCACCGC TCTTATCCCC ACTTTCCGCA 
AGGAACAGCA CTGCGAATGC GAACGCCAAT GCCGTACACC TTATGACCCA CTCGGGATGT 
GTGGTGGTGC TACCCTGTGA CCTGCGGACT CAGTTTGCGC GTCACGTTAC CATGAACAGC 
GTGAATCTCA TCCGGCGCTA CTGTGTGGAC CGTGTCTATC GGGAGGAGCG AGTGTTTAAT 
TTTCATCCCA AGCAAAGCTA CGACTGCTCT TTCGATATAA TTGCACCCAC AACTGGAAGT 
CATTTAGTTG ATGCCGAGCT GCTGTCGTTG GCTTTTGAAA TCACCAGTGA ACTGCCCCGA 
TTACGCGAAA AGAATTTGGC GATCCGCATG AACCACACTA ATCTCTTGAG GGCCATCCTC 
ATCTTCTGCA ATGTACCAAA GGCTCAGTAT GGAGCACTCT TCGAGGGCAC CATGGACTTC 
ATCGAGTCGC GCATCTCACG CTTCCAATTT CACTCGAGTA TTACCGGGAT TATGGAAAAG 
TCGCGAACAT CTGCTCAAAC TCTGATGGAC ATGTTGTTAG CTAACTTTCT GCTGACTGGT 
TCCAGAAGCA CTGTGGACGA TTCCGCGCTG AAATCTTTGA TGCGTGGAAA GGGAGAAGCA 
GCTTCACTGG CAAGGGGAGC ATTGCGGGAA CTTGAAACAG TTGTGGGACT GGCTTACAGT 
CTAGGCGTAA AGTGTCCTAT ACACATATGG GCTGGCTTGC CCATCAGCTT CGATCGTGCC 
AGCAACGGTG GAATTGTGTG GCAGATGACC GCTGACCTGA AGCCCAATCG CTCTGGCCAT 
CCTTCGGTTC TGGCGATTGG GGAGAGATAC GACTCTATGC TGCATGAATT CCAGAAGCAG 
GCGCAGAAGT TTAATCCCGC CATGCCAGCA CGTGGAGTTC TTAGTGGAGC GGGCTTGACG 
TTCTCCCTAG ACAAGCTGGT AGCTGCTGTT GGTGTGGAAT ACGCTAAGGA TTGTCGGGCG 
ATAGATGTGG GCATTTGCGT GTGTGGAACC CGTCCACCGC TAAAGGATGT GACCTATATC 
ATGCGTCTGC TGTGGTCGGT TGGCATCCGA TGTGGAATTG TGGAGGCCGC CAGCGAATTG 
GGAGATGAAG CACAGGATCT TGCCCGCCTT GGGGCGTTAC ATGTCATCCT GGTGGCGGAA 
AATGGCTCAT TGAGAGTGAG ATCTTTCGAG AGGGAACGAT TCCAGGAGCG TCACCTAACA 
AGAACCGAAC TGGTGGAGTT CATCCAGAAG ATGCTGCGAA GTGATGGACT CAATGGGACC 
ACCGTGGACA ACTTCAGCCA GTTGTCAGCC TTGGGTAGTG GTGACAACAG AAGCAGTGGT 
GGAAAGGAAA GAGAACGCGG CGAGAATGGT CTGAGCACCT CCGCATCGAA TGCCACCATC 
AAGAACAACT ATAGCCAGTT GCCGAATCTT CAGGTGACGT TTCTCACCCA CGACAAGCCA 
ACGGCGAACT ACAAAAGGCG GCTAGAGAAC CAGGTGGCGC AGCAGATGAG CTCAACGCTA 
TCGCAGTTCC TCAAGAAGGA GACCTTTGTG GTCCTGGTGG TGGAGTTGCC GCCTGCTGTT 
GTAAATGCCA TTGTGGGGGC CATTAATCCA CGGGAAATAC GCAAGCGAGA GACTGAACCG 
GAAATCAACT ATGTGATCGA GCGATTTTCG AAATACAAGC GCTACATCTC CGAGATAAAC 
GAAGAGGTTG TCGACTATCT GAGCGATGCC AAGACTCCCA TTGTGGCCTT GTACAGCATA 
TCCGATTCCT ACTACCGCGT CATCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477230.1
CDS	332..5101
Translation	MADEKAKESFRERQAQELEVIKSIFGCDVEDLRPQANPSLWKPTDIRIQLTPLRDSSNGLETYVCTKLHVTCPSKYPKLPPKISLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKPPKGSFYDQMLQDKQKRDQELQDIQRQRESLQRQTLIDEVERRKEMFKTEAKRRGEPRRSMSESNPRHPSSSESSENSSPYYRGHIYPSKCLDHRNTETLYFHKMGRQIQRGCCVGHSQRGCIAYTGIDMHCGQLLYITEWNIKYSQLEQPCIGGGKCHWSSESKCMGSHRVDEVMASIEKQVSSLSQLQHKNLVSYECVLCIKRKEGLLVYLVQDFLLGTSVFSISSSLGWCMDGARMVARGVLDALVFLHNKGVSHSHLLDTTVFMDNTGNVRVSDFSLVPNLLELLSGAGQSSSCGDLPALGALVESLMPTNSYEMRDFVDKCNSDRTLSASELLEHPFLRFYVDNGQQQVMPLPQQQHPNTVQRTGSAMPYQIPTLALSQSRLRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPARSRQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDADAAEMDKLLGGEWSQSQQDLSVKPAKSPQLGPTLEEDEDEEDSSSSMWNGYIPNMEDSDSDGIEFVDSNGKVAVYDDEEQEDSTRGKTNSPKPLMQVMYIQMEFCEKCTLRTAIDDNLFNDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDFGLATTSFLALQAHDAAPAPVNQITSAEDGTGTGKVGTTLYVAPELTGNASKSVYNQKVDMYTLGIILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKTVKMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALANPQSKAYKNLVARCLQQESDEVLEHTYHLGSSRAMKSWNSAIIIDDIVSLNPVIEFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSTANANANAVHLMTHSGCVVVLPCDLRTQFARHVTMNSVNLIRRYCVDRVYREERVFNFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFEITSELPRLREKNLAIRMNHTNLLRAILIFCNVPKAQYGALFEGTMDFIESRISRFQFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDDSALKSLMRGKGEAASLARGALRELETVVGLAYSLGVKCPIHIWAGLPISFDRASNGGIVWQMTADLKPNRSGHPSVLAIGERYDSMLHEFQKQAQKFNPAMPARGVLSGAGLTFSLDKLVAAVGVEYAKDCRAIDVGICVCGTRPPLKDVTYIMRLLWSVGIRCGIVEAASELGDEAQDLARLGALHVILVAENGSLRVRSFERERFQERHLTRTELVEFIQKMLRSDGLNGTTVDNFSQLSALGSGDNRSSGGKERERGENGLSTSASNATIKNNYSQLPNLQVTFLTHDKPTANYKRRLENQVAQQMSSTLSQFLKKETFVVLVVELPPAVVNAIVGAINPREIRKRETEPEINYVIERFSKYKRYISEINEEVVDYLSDAKTPIVALYSISDSYYRVII

Target ORF information:

RefSeq Version	NM_057882.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gcn2, transcript variant A (Gcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057882.4

ORF Insert Sequence:

ATGGCGGATG AAAAGGCCAA GGAATCCTTC CGCGAGCGGC AAGCGCAGGA GTTGGAGGTT 
ATAAAGTCCA TCTTTGGCTG TGATGTGGAG GACCTAAGAC CCCAGGCGAA TCCTTCCCTG 
TGGAAGCCAA CGGACATACG GATACAACTT ACCCCACTTC GAGACTCCTC GAACGGACTG 
GAAACCTATG TGTGCACCAA GCTGCACGTC ACCTGTCCAT CAAAGTATCC AAAATTGCCC 
CCTAAGATAT CTCTGGAGGA GTCCAAAGGC ATGTCGGATC AGCTGCTGGA GGCCCTACGC 
AACCAATTGC AGGCTCAATC CCAAGAGCTA CGTGGCGAAG TGATGATTTA TGAATTGGCC 
CAGACCGTTC AGGCCTTTCT CCTCGAACAC AACAAACCAC CAAAGGGATC ATTCTACGAC 
CAGATGCTCC AGGATAAACA GAAGCGCGAC CAGGAGCTAC AGGACATACA GCGGCAGCGG 
GAGTCCCTGC AACGCCAGAC GCTCATCGAC GAGGTGGAGC GGCGCAAAGA GATGTTCAAA 
ACGGAGGCTA AGAGGCGCGG CGAACCAAGG CGCAGCATGA GCGAGTCTAA CCCACGGCAT 
CCTAGCTCGT CGGAGAGCTC CGAGAACTCC TCGCCCTACT ATCGTGGTCA CATCTACCCC 
TCCAAATGCC TGGATCACCG CAACACGGAG ACGCTCTACT TCCACAAGAT GGGCAGGCAG 
ATTCAGCGTG GATGCTGCGT TGGTCACTCC CAACGAGGTT GCATCGCCTA CACTGGCATA 
GATATGCACT GCGGCCAGTT GCTCTACATA ACAGAATGGA ACATCAAGTA CAGCCAACTG 
GAGCAGCCAT GCATCGGCGG AGGCAAGTGC CACTGGAGCA GCGAGTCAAA GTGCATGGGC 
AGTCATCGTG TGGACGAAGT GATGGCCTCC ATTGAAAAGC AGGTGTCCTC ACTGTCCCAA 
CTACAGCACA AAAACCTCGT CTCGTACGAG TGCGTACTGT GCATCAAGCG AAAGGAAGGT 
CTACTTGTGT ATTTGGTCCA AGACTTCCTC CTTGGCACCA GCGTGTTCAG CATCTCCTCC 
TCGCTTGGCT GGTGCATGGA CGGCGCACGA ATGGTTGCGC GCGGCGTGCT GGACGCCTTA 
GTATTTCTTC ACAACAAGGG TGTATCCCAT AGTCACCTGC TCGACACCAC TGTTTTTATG 
GACAACACAG GCAATGTGAG GGTATCAGAC TTTTCGCTCG TTCCCAATCT TCTAGAACTT 
CTGAGCGGAG CTGGACAAAG CAGCAGTTGC GGGGACTTAC CCGCGTTAGG TGCCCTGGTG 
GAGAGTTTGA TGCCCACTAA CAGTTACGAG ATGCGCGACT TTGTAGACAA GTGTAACTCG 
GACCGAACTC TTTCCGCATC AGAGTTATTG GAGCATCCAT TCTTACGCTT TTACGTCGAC 
AATGGCCAGC AGCAGGTGAT GCCATTACCA CAGCAGCAAC ATCCTAACAC CGTCCAGCGC 
ACTGGATCCG CCATGCCGTA CCAGATACCG ACACTGGCCC TAAGCCAATC CCGCCTGCGA 
ACGGAATTCG AGGTGCTCAT GTACTTGGGA AAGGGTGCCT TTGGTGACGT GCTAAAGGTG 
CGCAACATTT TAGACAACCG AGAGTACGCC ATCAAGCGCA TACCATTGCC CGCGAGGAGT 
CGCCAGCTGT ACAAAAAGAT GACGCGCGAG GTGGAGCTTC TTTCGCGGCT GAATCATGAG 
AATGTAGTTC GATACTTTAA CAGTTGGATA GAAAGTGTAG ATGACGCAGA TGCCGCTGAG 
ATGGATAAGC TGCTGGGCGG TGAGTGGTCC CAGAGTCAGC AGGACCTTAG CGTAAAACCT 
GCCAAGTCAC CACAGCTGGG GCCCACTTTA GAGGAGGACG AGGATGAAGA GGACAGCTCA 
TCCAGCATGT GGAACGGTTA CATACCCAAC ATGGAGGACT CTGATTCGGA TGGCATTGAA 
TTTGTGGACT CCAACGGCAA GGTGGCTGTC TACGACGACG AAGAGCAGGA GGATTCCACC 
AGAGGCAAGA CCAATTCGCC AAAACCTTTG ATGCAGGTCA TGTACATCCA GATGGAGTTC 
TGTGAGAAAT GCACATTGCG CACCGCCATC GATGACAATC TCTTCAATGA CACGGATCGT 
CTGTGGCGCT TATTTCGGGA GATTGCCGAG GGTCTCGCGC ATATTCACCA GCAAGGCATT 
ATCCATCGGG ACCTAAAGCC CGTTAACATC TTCTTGGACT CGCACGATCA GATTAAGATC 
GGTGACTTTG GTCTGGCCAC CACCAGTTTT CTGGCTCTCC AAGCCCACGA CGCAGCGCCT 
GCGCCTGTTA ACCAGATAAC CAGTGCGGAA GACGGCACTG GTACTGGTAA GGTCGGCACC 
ACTCTATACG TGGCTCCCGA ATTGACAGGA AATGCTTCCA AGTCTGTGTA CAACCAGAAG 
GTTGATATGT ACACCCTGGG AATCATTCTT TTCGAGATGT GCCAACCGCC GTTCGACACA 
AGCATGGAGC GGGCTCAGAC CATTATGGCT CTCAGGAATG TTTCCATTAA TATACCGGAT 
GCTATGCTTA AGGATCCCAA GTATGAGAAG ACGGTGAAGA TGCTTCAGTG GCTTCTCAAC 
CACGATCCCG CCCAGAGACC TACTGCCGAG GAGCTGCTCA TCTCAGACCT GGTTCCACCA 
GCCCAGTTGG AGGCCAATGA GCTCCAAGAA ATGCTGCGTC ACGCCCTCGC TAATCCGCAG 
AGCAAGGCCT ACAAGAACCT AGTTGCCCGA TGTCTTCAGC AGGAGAGCGA CGAGGTGCTG 
GAACACACCT ACCACTTGGG AAGCAGTCGA GCTATGAAAT CCTGGAACTC GGCAATTATA 
ATCGATGATA TAGTATCCCT GAACCCAGTG ATTGAGTTTG TTAAGGCTAA GGTCGTGAAT 
TTATTCCGCA AGCATGGAGC TATAGAGGTG GACTCACCGC TCTTATCCCC ACTTTCCGCA 
AGGAACAGCA CTGCGAATGC GAACGCCAAT GCCGTACACC TTATGACCCA CTCGGGATGT 
GTGGTGGTGC TACCCTGTGA CCTGCGGACT CAGTTTGCGC GTCACGTTAC CATGAACAGC 
GTGAATCTCA TCCGGCGCTA CTGTGTGGAC CGTGTCTATC GGGAGGAGCG AGTGTTTAAT 
TTTCATCCCA AGCAAAGCTA CGACTGCTCT TTCGATATAA TTGCACCCAC AACTGGAAGT 
CATTTAGTTG ATGCCGAGCT GCTGTCGTTG GCTTTTGAAA TCACCAGTGA ACTGCCCCGA 
TTACGCGAAA AGAATTTGGC GATCCGCATG AACCACACTA ATCTCTTGAG GGCCATCCTC 
ATCTTCTGCA ATGTACCAAA GGCTCAGTAT GGAGCACTCT TCGAGGGCAC CATGGACTTC 
ATCGAGTCGC GCATCTCACG CTTCCAATTT CACTCGAGTA TTACCGGGAT TATGGAAAAG 
TCGCGAACAT CTGCTCAAAC TCTGATGGAC ATGTTGTTAG CTAACTTTCT GCTGACTGGT 
TCCAGAAGCA CTGTGGACGA TTCCGCGCTG AAATCTTTGA TGCGTGGAAA GGGAGAAGCA 
GCTTCACTGG CAAGGGGAGC ATTGCGGGAA CTTGAAACAG TTGTGGGACT GGCTTACAGT 
CTAGGCGTAA AGTGTCCTAT ACACATATGG GCTGGCTTGC CCATCAGCTT CGATCGTGCC 
AGCAACGGTG GAATTGTGTG GCAGATGACC GCTGACCTGA AGCCCAATCG CTCTGGCCAT 
CCTTCGGTTC TGGCGATTGG GGAGAGATAC GACTCTATGC TGCATGAATT CCAGAAGCAG 
GCGCAGAAGT TTAATCCCGC CATGCCAGCA CGTGGAGTTC TTAGTGGAGC GGGCTTGACG 
TTCTCCCTAG ACAAGCTGGT AGCTGCTGTT GGTGTGGAAT ACGCTAAGGA TTGTCGGGCG 
ATAGATGTGG GCATTTGCGT GTGTGGAACC CGTCCACCGC TAAAGGATGT GACCTATATC 
ATGCGTCTGC TGTGGTCGGT TGGCATCCGA TGTGGAATTG TGGAGGCCGC CAGCGAATTG 
GGAGATGAAG CACAGGATCT TGCCCGCCTT GGGGCGTTAC ATGTCATCCT GGTGGCGGAA 
AATGGCTCAT TGAGAGTGAG ATCTTTCGAG AGGGAACGAT TCCAGGAGCG TCACCTAACA 
AGAACCGAAC TGGTGGAGTT CATCCAGAAG ATGCTGCGAA GTGATGGACT CAATGGGACC 
ACCGTGGACA ACTTCAGCCA GTTGTCAGCC TTGGGTAGTG GTGACAACAG AAGCAGTGGT 
GGAAAGGAAA GAGAACGCGG CGAGAATGGT CTGAGCACCT CCGCATCGAA TGCCACCATC 
AAGAACAACT ATAGCCAGTT GCCGAATCTT CAGGTGACGT TTCTCACCCA CGACAAGCCA 
ACGGCGAACT ACAAAAGGCG GCTAGAGAAC CAGGTGGCGC AGCAGATGAG CTCAACGCTA 
TCGCAGTTCC TCAAGAAGGA GACCTTTGTG GTCCTGGTGG TGGAGTTGCC GCCTGCTGTT 
GTAAATGCCA TTGTGGGGGC CATTAATCCA CGGGAAATAC GCAAGCGAGA GACTGAACCG 
GAAATCAACT ATGTGATCGA GCGATTTTCG AAATACAAGC GCTACATCTC CGAGATAAAC 
GAAGAGGTTG TCGACTATCT GAGCGATGCC AAGACTCCCA TTGTGGCCTT GTACAGCATA 
TCCGATTCCT ACTACCGCGT CATCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gcn2 ( NM_001276213.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263142.1 Drosophila melanogaster Gcn2, transcript variant C (Gcn2), mRNA.

Price & Availability↑

CloneID	OFa07869
Clone ID Related Accession (Same CDS sequence)	NM_001276213.1
Accession Version	NM_001276213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gcn2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGATG AAAAGGCCAA GGAATCCTTC CGCGAGCGGC AAGCGCAGGA GTTGGAGGTT 
ATAAAGTCCA TCTTTGGCTG TGATGTGGAG GACCTAAGAC CCCAGGCGAA TCCTTCCCTG 
TGGAAGCCAA CGGACATACG GATACAACTT ACCCCACTTC GAGACTCCTC GAACGGACTG 
GAAACCTATG TGTGCACCAA GCTGCACGTC ACCTGTCCAT CAAAGTATCC AAAATTGCCC 
CCTAAGATAT CTCTGGAGGA GTCCAAAGGC ATGTCGGATC AGCTGCTGGA GGCCCTACGC 
AACCAATTGC AGGCTCAATC CCAAGAGCTA CGTGGCGAAG TGATGATTTA TGAATTGGCC 
CAGACCGTTC AGGCCTTTCT CCTCGAACAC AACAAACCAC CAAAGGGATC ATTCTACGAC 
CAGATGCTCC AGGATAAACA GAAGCGCGAC CAGGAGCTAC AGGACATACA GCGGCAGCGG 
GAGTCCCTGC AACGCCAGAC GCTCATCGAC GAGGTGGAGC GGCGCAAAGA GATGTTCAAA 
ACGGAGGCTA AGAGGCGCGG CGAACCAAGG CGCAGCATGA GCGAGTCTAA CCCACGGCAT 
CCTAGCTCGT CGGAGAGCTC CGAGAACTCC TCGCCCTACT ATCGTGGTCA CATCTACCCC 
TCCAAATGCC TGGATCACCG CAACACGGAG ACGCTCTACT TCCACAAGAT GGGCAGGCAG 
ATTCAGCGTG GATGCTGCGT TGGTGAGGGT CACTCCCAAC GAGGTTGCAT CGCCTACACT 
GGCATAGATA TGCACTGCGG CCAGTTGCTC TACATAACAG AATGGAACAT CAAGTACAGC 
CAACTGGAGC AGCCATGCAT CGGCGGAGGC AAGTGCCACT GGAGCAGCGA GTCAAAGTGC 
ATGGGCAGTC ATCGTGTGGA CGAAGTGATG GCCTCCATTG AAAAGCAGGT GTCCTCACTG 
TCCCAACTAC AGCACAAAAA CCTCGTCTCG TACGAGTGCG TACTGTGCAT CAAGCGAAAG 
GAAGGTCTAC TTGTGTATTT GGTCCAAGAC TTCCTCCTTG GCACCAGCGT GTTCAGCATC 
TCCTCCTCGC TTGGCTGGTG CATGGACGGC GCACGAATGG TTGCGCGCGG CGTGCTGGAC 
GCCTTAGTAT TTCTTCACAA CAAGGGTGTA TCCCATAGTC ACCTGCTCGA CACCACTGTT 
TTTATGGACA ACACAGGCAA TGTGAGGGTA TCAGACTTTT CGCTCGTTCC CAATCTTCTA 
GAACTTCTGA GCGGAGCTGG ACAAAGCAGC AGTTGCGGGG ACTTACCCGC GTTAGGTGCC 
CTGGTGGAGA GTTTGATGCC CACTAACAGT TACGAGATGC GCGACTTTGT AGACAAGTGT 
AACTCGGACC GAACTCTTTC CGCATCAGAG TTATTGGAGC ATCCATTCTT ACGCTTTTAC 
GTCGACAATG GCCAGCAGCA GGTGATGCCA TTACCACAGC AGCAACATCC TAACACCGTC 
CAGCGCACTG GATCCGCCAT GCCGTACCAG ATACCGACAC TGGCCCTAAG CCAATCCCGC 
CTGCGAACGG AATTCGAGGT GCTCATGTAC TTGGGAAAGG GTGCCTTTGG TGACGTGCTA 
AAGGTGCGCA ACATTTTAGA CAACCGAGAG TACGCCATCA AGCGCATACC ATTGCCCGCG 
AGGAGTCGCC AGCTGTACAA AAAGATGACG CGCGAGGTGG AGCTTCTTTC GCGGCTGAAT 
CATGAGAATG TAGTTCGATA CTTTAACAGT TGGATAGAAA GTGTAGATGA CGCAGATGCC 
GCTGAGATGG ATAAGCTGCT GGGCGGTGAG TGGTCCCAGA GTCAGCAGGA CCTTAGCGTA 
AAACCTGCCA AGTCACCACA GCTGGGGCCC ACTTTAGAGG AGGACGAGGA TGAAGAGGAC 
AGCTCATCCA GCATGTGGAA CGGTTACATA CCCAACATGG AGGACTCTGA TTCGGATGGC 
ATTGAATTTG TGGACTCCAA CGGCAAGGTG GCTGTCTACG ACGACGAAGA GCAGGAGGAT 
TCCACCAGAG GCAAGACCAA TTCGCCAAAA CCTTTGATGC AGGTCATGTA CATCCAGATG 
GAGTTCTGTG AGAAATGCAC ATTGCGCACC GCCATCGATG ACAATCTCTT CAATGACACG 
GATCGTCTGT GGCGCTTATT TCGGGAGATT GCCGAGGGTC TCGCGCATAT TCACCAGCAA 
GGCATTATCC ATCGGGACCT AAAGCCCGTT AACATCTTCT TGGACTCGCA CGATCAGATT 
AAGATCGGTG ACTTTGGTCT GGCCACCACC AGTTTTCTGG CTCTCCAAGC CCACGACGCA 
GCGCCTGCGC CTGTTAACCA GATAACCAGT GCGGAAGACG GCACTGGTAC TGGTAAGGTC 
GGCACCACTC TATACGTGGC TCCCGAATTG ACAGGAAATG CTTCCAAGTC TGTGTACAAC 
CAGAAGGTTG ATATGTACAC CCTGGGAATC ATTCTTTTCG AGATGTGCCA ACCGCCGTTC 
GACACAAGCA TGGAGCGGGC TCAGACCATT ATGGCTCTCA GGAATGTTTC CATTAATATA 
CCGGATGCTA TGCTTAAGGA TCCCAAGTAT GAGAAGACGG TGAAGATGCT TCAGTGGCTT 
CTCAACCACG ATCCCGCCCA GAGACCTACT GCCGAGGAGC TGCTCATCTC AGACCTGGTT 
CCACCAGCCC AGTTGGAGGC CAATGAGCTC CAAGAAATGC TGCGTCACGC CCTCGCTAAT 
CCGCAGAGCA AGGCCTACAA GAACCTAGTT GCCCGATGTC TTCAGCAGGA GAGCGACGAG 
GTGCTGGAAC ACACCTACCA CTTGGGAAGC AGTCGAGCTA TGAAATCCTG GAACTCGGCA 
ATTATAATCG ATGATATAGT ATCCCTGAAC CCAGTGATTG AGTTTGTTAA GGCTAAGGTC 
GTGAATTTAT TCCGCAAGCA TGGAGCTATA GAGGTGGACT CACCGCTCTT ATCCCCACTT 
TCCGCAAGGA ACAGCACTGC GAATGCGAAC GCCAATGCCG TACACCTTAT GACCCACTCG 
GGATGTGTGG TGGTGCTACC CTGTGACCTG CGGACTCAGT TTGCGCGTCA CGTTACCATG 
AACAGCGTGA ATCTCATCCG GCGCTACTGT GTGGACCGTG TCTATCGGGA GGAGCGAGTG 
TTTAATTTTC ATCCCAAGCA AAGCTACGAC TGCTCTTTCG ATATAATTGC ACCCACAACT 
GGAAGTCATT TAGTTGATGC CGAGCTGCTG TCGTTGGCTT TTGAAATCAC CAGTGAACTG 
CCCCGATTAC GCGAAAAGAA TTTGGCGATC CGCATGAACC ACACTAATCT CTTGAGGGCC 
ATCCTCATCT TCTGCAATGT ACCAAAGGCT CAGTATGGAG CACTCTTCGA GGGCACCATG 
GACTTCATCG AGTCGCGCAT CTCACGCTTC CAATTTCACT CGAGTATTAC CGGGATTATG 
GAAAAGTCGC GAACATCTGC TCAAACTCTG ATGGACATGT TGTTAGCTAA CTTTCTGCTG 
ACTGGTTCCA GAAGCACTGT GGACGATTCC GCGCTGAAAT CTTTGATGCG TGGAAAGGGA 
GAAGCAGCTT CACTGGCAAG GGGAGCATTG CGGGAACTTG AAACAGTTGT GGGACTGGCT 
TACAGTCTAG GCGTAAAGTG TCCTATACAC ATATGGGCTG GCTTGCCCAT CAGCTTCGAT 
CGTGCCAGCA ACGGTGGAAT TGTGTGGCAG ATGACCGCTG ACCTGAAGCC CAATCGCTCT 
GGCCATCCTT CGGTTCTGGC GATTGGGGAG AGATACGACT CTATGCTGCA TGAATTCCAG 
AAGCAGGCGC AGAAGTTTAA TCCCGCCATG CCAGCACGTG GAGTTCTTAG TGGAGCGGGC 
TTGACGTTCT CCCTAGACAA GCTGGTAGCT GCTGTTGGTG TGGAATACGC TAAGGATTGT 
CGGGCGATAG ATGTGGGCAT TTGCGTGTGT GGAACCCGTC CACCGCTAAA GGATGTGACC 
TATATCATGC GTCTGCTGTG GTCGGTTGGC ATCCGATGTG GAATTGTGGA GGCCGCCAGC 
GAATTGGGAG ATGAAGCACA GGATCTTGCC CGCCTTGGGG CGTTACATGT CATCCTGGTG 
GCGGAAAATG GCTCATTGAG AGTGAGATCT TTCGAGAGGG AACGATTCCA GGAGCGTCAC 
CTAACAAGAA CCGAACTGGT GGAGTTCATC CAGAAGATGC TGCGAAGTGA TGGACTCAAT 
GGGACCACCG TGGACAACTT CAGCCAGTTG TCAGCCTTGG GTAGTGGTGA CAACAGAAGC 
AGTGGTGGAA AGGAAAGAGA ACGCGGCGAG AATGGTCTGA GCACCTCCGC ATCGAATGCC 
ACCATCAAGA ACAACTATAG CCAGTTGCCG AATCTTCAGG TGACGTTTCT CACCCACGAC 
AAGCCAACGG CGAACTACAA AAGGCGGCTA GAGAACCAGG TGGCGCAGCA GATGAGCTCA 
ACGCTATCGC AGTTCCTCAA GAAGGAGACC TTTGTGGTCC TGGTGGTGGA GTTGCCGCCT 
GCTGTTGTAA ATGCCATTGT GGGGGCCATT AATCCACGGG AAATACGCAA GCGAGAGACT 
GAACCGGAAA TCAACTATGT GATCGAGCGA TTTTCGAAAT ACAAGCGCTA CATCTCCGAG 
ATAAACGAAG AGGTTGTCGA CTATCTGAGC GATGCCAAGA CTCCCATTGT GGCCTTGTAC 
AGCATATCCG ATTCCTACTA CCGCGTCATC ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263142.1
CDS	332..5107
Translation	MADEKAKESFRERQAQELEVIKSIFGCDVEDLRPQANPSLWKPTDIRIQLTPLRDSSNGLETYVCTKLHVTCPSKYPKLPPKISLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKPPKGSFYDQMLQDKQKRDQELQDIQRQRESLQRQTLIDEVERRKEMFKTEAKRRGEPRRSMSESNPRHPSSSESSENSSPYYRGHIYPSKCLDHRNTETLYFHKMGRQIQRGCCVGEGHSQRGCIAYTGIDMHCGQLLYITEWNIKYSQLEQPCIGGGKCHWSSESKCMGSHRVDEVMASIEKQVSSLSQLQHKNLVSYECVLCIKRKEGLLVYLVQDFLLGTSVFSISSSLGWCMDGARMVARGVLDALVFLHNKGVSHSHLLDTTVFMDNTGNVRVSDFSLVPNLLELLSGAGQSSSCGDLPALGALVESLMPTNSYEMRDFVDKCNSDRTLSASELLEHPFLRFYVDNGQQQVMPLPQQQHPNTVQRTGSAMPYQIPTLALSQSRLRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPARSRQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDADAAEMDKLLGGEWSQSQQDLSVKPAKSPQLGPTLEEDEDEEDSSSSMWNGYIPNMEDSDSDGIEFVDSNGKVAVYDDEEQEDSTRGKTNSPKPLMQVMYIQMEFCEKCTLRTAIDDNLFNDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDFGLATTSFLALQAHDAAPAPVNQITSAEDGTGTGKVGTTLYVAPELTGNASKSVYNQKVDMYTLGIILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKTVKMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALANPQSKAYKNLVARCLQQESDEVLEHTYHLGSSRAMKSWNSAIIIDDIVSLNPVIEFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSTANANANAVHLMTHSGCVVVLPCDLRTQFARHVTMNSVNLIRRYCVDRVYREERVFNFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFEITSELPRLREKNLAIRMNHTNLLRAILIFCNVPKAQYGALFEGTMDFIESRISRFQFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDDSALKSLMRGKGEAASLARGALRELETVVGLAYSLGVKCPIHIWAGLPISFDRASNGGIVWQMTADLKPNRSGHPSVLAIGERYDSMLHEFQKQAQKFNPAMPARGVLSGAGLTFSLDKLVAAVGVEYAKDCRAIDVGICVCGTRPPLKDVTYIMRLLWSVGIRCGIVEAASELGDEAQDLARLGALHVILVAENGSLRVRSFERERFQERHLTRTELVEFIQKMLRSDGLNGTTVDNFSQLSALGSGDNRSSGGKERERGENGLSTSASNATIKNNYSQLPNLQVTFLTHDKPTANYKRRLENQVAQQMSSTLSQFLKKETFVVLVVELPPAVVNAIVGAINPREIRKRETEPEINYVIERFSKYKRYISEINEEVVDYLSDAKTPIVALYSISDSYYRVII

Target ORF information:

RefSeq Version	NM_001276213.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gcn2, transcript variant C (Gcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276213.1

ORF Insert Sequence:

ATGGCGGATG AAAAGGCCAA GGAATCCTTC CGCGAGCGGC AAGCGCAGGA GTTGGAGGTT 
ATAAAGTCCA TCTTTGGCTG TGATGTGGAG GACCTAAGAC CCCAGGCGAA TCCTTCCCTG 
TGGAAGCCAA CGGACATACG GATACAACTT ACCCCACTTC GAGACTCCTC GAACGGACTG 
GAAACCTATG TGTGCACCAA GCTGCACGTC ACCTGTCCAT CAAAGTATCC AAAATTGCCC 
CCTAAGATAT CTCTGGAGGA GTCCAAAGGC ATGTCGGATC AGCTGCTGGA GGCCCTACGC 
AACCAATTGC AGGCTCAATC CCAAGAGCTA CGTGGCGAAG TGATGATTTA TGAATTGGCC 
CAGACCGTTC AGGCCTTTCT CCTCGAACAC AACAAACCAC CAAAGGGATC ATTCTACGAC 
CAGATGCTCC AGGATAAACA GAAGCGCGAC CAGGAGCTAC AGGACATACA GCGGCAGCGG 
GAGTCCCTGC AACGCCAGAC GCTCATCGAC GAGGTGGAGC GGCGCAAAGA GATGTTCAAA 
ACGGAGGCTA AGAGGCGCGG CGAACCAAGG CGCAGCATGA GCGAGTCTAA CCCACGGCAT 
CCTAGCTCGT CGGAGAGCTC CGAGAACTCC TCGCCCTACT ATCGTGGTCA CATCTACCCC 
TCCAAATGCC TGGATCACCG CAACACGGAG ACGCTCTACT TCCACAAGAT GGGCAGGCAG 
ATTCAGCGTG GATGCTGCGT TGGTGAGGGT CACTCCCAAC GAGGTTGCAT CGCCTACACT 
GGCATAGATA TGCACTGCGG CCAGTTGCTC TACATAACAG AATGGAACAT CAAGTACAGC 
CAACTGGAGC AGCCATGCAT CGGCGGAGGC AAGTGCCACT GGAGCAGCGA GTCAAAGTGC 
ATGGGCAGTC ATCGTGTGGA CGAAGTGATG GCCTCCATTG AAAAGCAGGT GTCCTCACTG 
TCCCAACTAC AGCACAAAAA CCTCGTCTCG TACGAGTGCG TACTGTGCAT CAAGCGAAAG 
GAAGGTCTAC TTGTGTATTT GGTCCAAGAC TTCCTCCTTG GCACCAGCGT GTTCAGCATC 
TCCTCCTCGC TTGGCTGGTG CATGGACGGC GCACGAATGG TTGCGCGCGG CGTGCTGGAC 
GCCTTAGTAT TTCTTCACAA CAAGGGTGTA TCCCATAGTC ACCTGCTCGA CACCACTGTT 
TTTATGGACA ACACAGGCAA TGTGAGGGTA TCAGACTTTT CGCTCGTTCC CAATCTTCTA 
GAACTTCTGA GCGGAGCTGG ACAAAGCAGC AGTTGCGGGG ACTTACCCGC GTTAGGTGCC 
CTGGTGGAGA GTTTGATGCC CACTAACAGT TACGAGATGC GCGACTTTGT AGACAAGTGT 
AACTCGGACC GAACTCTTTC CGCATCAGAG TTATTGGAGC ATCCATTCTT ACGCTTTTAC 
GTCGACAATG GCCAGCAGCA GGTGATGCCA TTACCACAGC AGCAACATCC TAACACCGTC 
CAGCGCACTG GATCCGCCAT GCCGTACCAG ATACCGACAC TGGCCCTAAG CCAATCCCGC 
CTGCGAACGG AATTCGAGGT GCTCATGTAC TTGGGAAAGG GTGCCTTTGG TGACGTGCTA 
AAGGTGCGCA ACATTTTAGA CAACCGAGAG TACGCCATCA AGCGCATACC ATTGCCCGCG 
AGGAGTCGCC AGCTGTACAA AAAGATGACG CGCGAGGTGG AGCTTCTTTC GCGGCTGAAT 
CATGAGAATG TAGTTCGATA CTTTAACAGT TGGATAGAAA GTGTAGATGA CGCAGATGCC 
GCTGAGATGG ATAAGCTGCT GGGCGGTGAG TGGTCCCAGA GTCAGCAGGA CCTTAGCGTA 
AAACCTGCCA AGTCACCACA GCTGGGGCCC ACTTTAGAGG AGGACGAGGA TGAAGAGGAC 
AGCTCATCCA GCATGTGGAA CGGTTACATA CCCAACATGG AGGACTCTGA TTCGGATGGC 
ATTGAATTTG TGGACTCCAA CGGCAAGGTG GCTGTCTACG ACGACGAAGA GCAGGAGGAT 
TCCACCAGAG GCAAGACCAA TTCGCCAAAA CCTTTGATGC AGGTCATGTA CATCCAGATG 
GAGTTCTGTG AGAAATGCAC ATTGCGCACC GCCATCGATG ACAATCTCTT CAATGACACG 
GATCGTCTGT GGCGCTTATT TCGGGAGATT GCCGAGGGTC TCGCGCATAT TCACCAGCAA 
GGCATTATCC ATCGGGACCT AAAGCCCGTT AACATCTTCT TGGACTCGCA CGATCAGATT 
AAGATCGGTG ACTTTGGTCT GGCCACCACC AGTTTTCTGG CTCTCCAAGC CCACGACGCA 
GCGCCTGCGC CTGTTAACCA GATAACCAGT GCGGAAGACG GCACTGGTAC TGGTAAGGTC 
GGCACCACTC TATACGTGGC TCCCGAATTG ACAGGAAATG CTTCCAAGTC TGTGTACAAC 
CAGAAGGTTG ATATGTACAC CCTGGGAATC ATTCTTTTCG AGATGTGCCA ACCGCCGTTC 
GACACAAGCA TGGAGCGGGC TCAGACCATT ATGGCTCTCA GGAATGTTTC CATTAATATA 
CCGGATGCTA TGCTTAAGGA TCCCAAGTAT GAGAAGACGG TGAAGATGCT TCAGTGGCTT 
CTCAACCACG ATCCCGCCCA GAGACCTACT GCCGAGGAGC TGCTCATCTC AGACCTGGTT 
CCACCAGCCC AGTTGGAGGC CAATGAGCTC CAAGAAATGC TGCGTCACGC CCTCGCTAAT 
CCGCAGAGCA AGGCCTACAA GAACCTAGTT GCCCGATGTC TTCAGCAGGA GAGCGACGAG 
GTGCTGGAAC ACACCTACCA CTTGGGAAGC AGTCGAGCTA TGAAATCCTG GAACTCGGCA 
ATTATAATCG ATGATATAGT ATCCCTGAAC CCAGTGATTG AGTTTGTTAA GGCTAAGGTC 
GTGAATTTAT TCCGCAAGCA TGGAGCTATA GAGGTGGACT CACCGCTCTT ATCCCCACTT 
TCCGCAAGGA ACAGCACTGC GAATGCGAAC GCCAATGCCG TACACCTTAT GACCCACTCG 
GGATGTGTGG TGGTGCTACC CTGTGACCTG CGGACTCAGT TTGCGCGTCA CGTTACCATG 
AACAGCGTGA ATCTCATCCG GCGCTACTGT GTGGACCGTG TCTATCGGGA GGAGCGAGTG 
TTTAATTTTC ATCCCAAGCA AAGCTACGAC TGCTCTTTCG ATATAATTGC ACCCACAACT 
GGAAGTCATT TAGTTGATGC CGAGCTGCTG TCGTTGGCTT TTGAAATCAC CAGTGAACTG 
CCCCGATTAC GCGAAAAGAA TTTGGCGATC CGCATGAACC ACACTAATCT CTTGAGGGCC 
ATCCTCATCT TCTGCAATGT ACCAAAGGCT CAGTATGGAG CACTCTTCGA GGGCACCATG 
GACTTCATCG AGTCGCGCAT CTCACGCTTC CAATTTCACT CGAGTATTAC CGGGATTATG 
GAAAAGTCGC GAACATCTGC TCAAACTCTG ATGGACATGT TGTTAGCTAA CTTTCTGCTG 
ACTGGTTCCA GAAGCACTGT GGACGATTCC GCGCTGAAAT CTTTGATGCG TGGAAAGGGA 
GAAGCAGCTT CACTGGCAAG GGGAGCATTG CGGGAACTTG AAACAGTTGT GGGACTGGCT 
TACAGTCTAG GCGTAAAGTG TCCTATACAC ATATGGGCTG GCTTGCCCAT CAGCTTCGAT 
CGTGCCAGCA ACGGTGGAAT TGTGTGGCAG ATGACCGCTG ACCTGAAGCC CAATCGCTCT 
GGCCATCCTT CGGTTCTGGC GATTGGGGAG AGATACGACT CTATGCTGCA TGAATTCCAG 
AAGCAGGCGC AGAAGTTTAA TCCCGCCATG CCAGCACGTG GAGTTCTTAG TGGAGCGGGC 
TTGACGTTCT CCCTAGACAA GCTGGTAGCT GCTGTTGGTG TGGAATACGC TAAGGATTGT 
CGGGCGATAG ATGTGGGCAT TTGCGTGTGT GGAACCCGTC CACCGCTAAA GGATGTGACC 
TATATCATGC GTCTGCTGTG GTCGGTTGGC ATCCGATGTG GAATTGTGGA GGCCGCCAGC 
GAATTGGGAG ATGAAGCACA GGATCTTGCC CGCCTTGGGG CGTTACATGT CATCCTGGTG 
GCGGAAAATG GCTCATTGAG AGTGAGATCT TTCGAGAGGG AACGATTCCA GGAGCGTCAC 
CTAACAAGAA CCGAACTGGT GGAGTTCATC CAGAAGATGC TGCGAAGTGA TGGACTCAAT 
GGGACCACCG TGGACAACTT CAGCCAGTTG TCAGCCTTGG GTAGTGGTGA CAACAGAAGC 
AGTGGTGGAA AGGAAAGAGA ACGCGGCGAG AATGGTCTGA GCACCTCCGC ATCGAATGCC 
ACCATCAAGA ACAACTATAG CCAGTTGCCG AATCTTCAGG TGACGTTTCT CACCCACGAC 
AAGCCAACGG CGAACTACAA AAGGCGGCTA GAGAACCAGG TGGCGCAGCA GATGAGCTCA 
ACGCTATCGC AGTTCCTCAA GAAGGAGACC TTTGTGGTCC TGGTGGTGGA GTTGCCGCCT 
GCTGTTGTAA ATGCCATTGT GGGGGCCATT AATCCACGGG AAATACGCAA GCGAGAGACT 
GAACCGGAAA TCAACTATGT GATCGAGCGA TTTTCGAAAT ACAAGCGCTA CATCTCCGAG 
ATAAACGAAG AGGTTGTCGA CTATCTGAGC GATGCCAAGA CTCCCATTGT GGCCTTGTAC 
AGCATATCCG ATTCCTACTA CCGCGTCATC ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gcn2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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