dco cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dco
Entrez Gene ID	43673
Full Name	discs overgrown
Synonyms	0538/13,0915/10,1396/02,1447/01,1460/09,CG2048,CK1delta/epsilon,CK1epsilon,CKIepsilon,DBT,DBT/CK1epsilon,DCO,DCO/CKIe,Dbt,Dco,Dco/CK1,Dmel\CG2048,ck1epsilon,dCKIepsilon,dbt,dco-1,ddbt,l(3)S053813,l(3)S144701,l(3)dco,l(3)dco-1,l(3)discs overgrown,l(3)discs overgrown-1,l(3)j3B9,l(3)rK215
General protein information	Preferred Names discs overgrown Names CG2048 gene product from transcript CG2048-RD CG2048-PA CG2048-PB CG2048-PC CG2048-PD casein kinase 1 casein kinase I epsilon casein kinase iepsilon dco-PA dco-PB dco-PC dco-PD disc overgrown double time double-time doubletime
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100B2-100B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_170535.2	NP_733414.1	discs overgrown, isoform A
NM_079863.3	NP_524602.1	discs overgrown, isoform C
NM_001276203.1	NP_001263132.1	discs overgrown, isoform D
NM_001369991.1	NP_001356928.1	discs overgrown, isoform E
NM_170536.3	NP_733415.1	discs overgrown, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CSNK1E	XP_850168.1
Xenopus tropicalis (tropical clawed frog)	csnk1e	NP_001011137.1
Pan troglodytes (chimpanzee)	CSNK1E	XP_003317283.1
Gallus gallus (chicken)	CSNK1E	NP_989708.2
Arabidopsis thaliana (thale cress)	ckl12	NP_680447.1
Arabidopsis thaliana (thale cress)	CK1	NP_194340.1
	CSNK1E	XP_001093672.1
Drosophila melanogaster (fruit fly)	dco	NP_001263132.1
Homo sapiens (human)	CSNK1E	NP_001885.1
Mus musculus (house mouse)	Csnk1e	NP_038795.3
Rattus norvegicus (Norway rat)	Csnk1e	NP_113805.1
Danio rerio (zebrafish)	csnk1e	NP_997912.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate that Bride of Doubletime (BDBT) interacts with doubletime(DBT)in a manner requiring the DBT nuclear localization signal for nuclear localization and that the interaction is required to produce a fully functional circadian complex
Title: A Doubletime Nuclear Localization Signal Mediates an Interaction with Bride of Doubletime to Promote Circadian Function.

Data show that the HSP-90 cochaperone spaghetti protein (SPAG) antagonizes casein kinase I epsilon DBT autophosphorylation in S2 cells
Title: Drosophila DBT Autophosphorylation of Its C-Terminal Domain Antagonized by SPAG and Involved in UV-Induced Apoptosis.

we show that spag knock-down or reduced Dbt activity in circadian cells leads to longer circadian periods and expression of activated initiator caspase only during the middle of the day.
Title: Drosophila spaghetti and doubletime link the circadian clock and light to caspases, apoptosis and tauopathy.

Responses of the clock to light via the regulated translation of DBT.
Title: Translational regulation of the DOUBLETIME/CKI?/? kinase by LARK contributes to circadian period modulation.

Data demonstrate that overall reduction of DBT causes long and arrhythmic behavior revealing an unexpected role in promoting synchrony of the circadian clock network.
Title: Casein kinase 1 promotes synchrony of the circadian clock network.

The following dco gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dco cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06727	NM_001369991.1 Latest version!	Drosophila melanogaster discs overgrown (dco), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa06727	NM_170535.2 Latest version!	Drosophila melanogaster discs overgrown (dco), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa06727	NM_079863.3 Latest version!	Drosophila melanogaster discs overgrown (dco), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa06727	NM_001276203.1 Latest version!	Drosophila melanogaster discs overgrown (dco), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa06727	NM_170536.3 Latest version!	Drosophila melanogaster discs overgrown (dco), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dco ( NM_170535.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733414.1 Drosophila melanogaster discs overgrown (dco), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06727
Clone ID Related Accession (Same CDS sequence)	NM_170535.2 , NM_079863.3 , NM_001276203.1 , NM_001369991.1 , NM_170536.3
Accession Version	NM_170535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	discs overgrown, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170535.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCTGC GCGTGGGTAA CAAATATCGC CTGGGCCGCA AGATAGGATC GGGATCGTTC 
GGCGACATCT ACCTGGGCAC CACGATCAAC ACTGGCGAGG AGGTGGCCAT CAAGCTGGAG 
TGCATCCGCA CCAAACACCC CCAGCTGCAC ATCGAGTCAA AGTTCTACAA GACGATGCAG 
GGTGGCATAG GCATACCCCG CATAATCTGG TGCGGCAGCG AGGGCGACTA CAATGTGATG 
GTGATGGAGC TACTCGGACC CTCGCTGGAG GACCTCTTCA ACTTTTGTTC ACGCCGCTTT 
TCGTTGAAGA CGGTTCTGCT GCTGGCGGAC CAGATGATCT CCCGCATCGA TTACATACAC 
TCGCGGGACT TCATCCATCG CGACATCAAG CCGGATAACT TCCTCATGGG TCTTGGCAAA 
AAGGGCAACC TGGTGTACAT CATTGACTTT GGCCTGGCCA AGAAATTCCG CGATGCCCGG 
TCGCTGAAGC ACATTCCCTA TCGGGAAAAC AAGAACCTCA CGGGCACTGC CCGCTATGCC 
TCCATCAACA CACATTTGGG CATTGAGCAA TCGCGTCGTG ACGACCTGGA GTCCCTGGGC 
TACGTCCTAA TGTACTTCAA TCTGGGCGCC TTGCCCTGGC AGGGCTTAAA GGCAGCCAAC 
AAGAGGCAAA AGTACGAGAG GATCTCGGAG AAGAAGCTGT CCACCTCGAT TGTGGTGCTG 
TGCAAGGGCT TCCCCAGCGA GTTCGTCAAC TATCTGAACT TCTGTCGCCA GATGCATTTC 
GACCAGCGTC CCGATTACTG CCACCTGCGC AAACTCTTCC GCAACTTGTT CCACCGTTTG 
GGCTTCACTT ACGACTATGT GTTTGACTGG AACCTGCTTA AGTTTGGCGG ACCTCGCAAT 
CCCCAGGCGA TTCAGCAGGC GCAGGACGGA GCGGACGGCC AGGCGGGTCA TGATGCGGTG 
GCCGCAGCAG CGGCGGTGGC AGCAGCGGCA GCCGCCTCCT CGCATCAACA ACAGCAGCAC 
AAGGTCAATG CGGCGCTGGG TGGCGGAGGA GGCAGTGCAG CGCAACAGCA ACTCCAGGGC 
GGCCAAACGC TGGCGATGCT GGGCGGCAAT GGAGGCGGTA ACGGCAGCCA ACTGATCGGC 
GGCAACGGAC TCAACATGGA CGACTCGATG GCGGCCACCA ACTCGTCGAG ACCGCCGTAC 
GACACGCCGG AGCGTCGGCC TTCGATACGG ATGCGGCAGG GAGGCGGTGG TGGCGGCGGT 
GGAGTGGGCG TAGGCGGTAT GCCGAGCGGC GGAGGGGGCG GTGGCGTGGG GAACGCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733414.1
CDS	315..1637
Translation	MELRVGNKYRLGRKIGSGSFGDIYLGTTINTGEEVAIKLECIRTKHPQLHIESKFYKTMQGGIGIPRIIWCGSEGDYNVMVMELLGPSLEDLFNFCSRRFSLKTVLLLADQMISRIDYIHSRDFIHRDIKPDNFLMGLGKKGNLVYIIDFGLAKKFRDARSLKHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGALPWQGLKAANKRQKYERISEKKLSTSIVVLCKGFPSEFVNYLNFCRQMHFDQRPDYCHLRKLFRNLFHRLGFTYDYVFDWNLLKFGGPRNPQAIQQAQDGADGQAGHDAVAAAAAVAAAAAASSHQQQQHKVNAALGGGGGSAAQQQLQGGQTLAMLGGNGGGNGSQLIGGNGLNMDDSMAATNSSRPPYDTPERRPSIRMRQGGGGGGGGVGVGGMPSGGGGGGVGNAK

Target ORF information:

RefSeq Version	NM_170535.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster discs overgrown (dco), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170535.2

ORF Insert Sequence:

ATGGAGCTGC GCGTGGGTAA CAAATATCGC CTGGGCCGCA AGATAGGATC GGGATCGTTC 
GGCGACATCT ACCTGGGCAC CACGATCAAC ACTGGCGAGG AGGTGGCCAT CAAGCTGGAG 
TGCATCCGCA CCAAACACCC CCAGCTGCAC ATCGAGTCAA AGTTCTACAA GACGATGCAG 
GGTGGCATAG GCATACCCCG CATAATCTGG TGCGGCAGCG AGGGCGACTA CAATGTGATG 
GTGATGGAGC TACTCGGACC CTCGCTGGAG GACCTCTTCA ACTTTTGTTC ACGCCGCTTT 
TCGTTGAAGA CGGTTCTGCT GCTGGCGGAC CAGATGATCT CCCGCATCGA TTACATACAC 
TCGCGGGACT TCATCCATCG CGACATCAAG CCGGATAACT TCCTCATGGG TCTTGGCAAA 
AAGGGCAACC TGGTGTACAT CATTGACTTT GGCCTGGCCA AGAAATTCCG CGATGCCCGG 
TCGCTGAAGC ACATTCCCTA TCGGGAAAAC AAGAACCTCA CGGGCACTGC CCGCTATGCC 
TCCATCAACA CACATTTGGG CATTGAGCAA TCGCGTCGTG ACGACCTGGA GTCCCTGGGC 
TACGTCCTAA TGTACTTCAA TCTGGGCGCC TTGCCCTGGC AGGGCTTAAA GGCAGCCAAC 
AAGAGGCAAA AGTACGAGAG GATCTCGGAG AAGAAGCTGT CCACCTCGAT TGTGGTGCTG 
TGCAAGGGCT TCCCCAGCGA GTTCGTCAAC TATCTGAACT TCTGTCGCCA GATGCATTTC 
GACCAGCGTC CCGATTACTG CCACCTGCGC AAACTCTTCC GCAACTTGTT CCACCGTTTG 
GGCTTCACTT ACGACTATGT GTTTGACTGG AACCTGCTTA AGTTTGGCGG ACCTCGCAAT 
CCCCAGGCGA TTCAGCAGGC GCAGGACGGA GCGGACGGCC AGGCGGGTCA TGATGCGGTG 
GCCGCAGCAG CGGCGGTGGC AGCAGCGGCA GCCGCCTCCT CGCATCAACA ACAGCAGCAC 
AAGGTCAATG CGGCGCTGGG TGGCGGAGGA GGCAGTGCAG CGCAACAGCA ACTCCAGGGC 
GGCCAAACGC TGGCGATGCT GGGCGGCAAT GGAGGCGGTA ACGGCAGCCA ACTGATCGGC 
GGCAACGGAC TCAACATGGA CGACTCGATG GCGGCCACCA ACTCGTCGAG ACCGCCGTAC 
GACACGCCGG AGCGTCGGCC TTCGATACGG ATGCGGCAGG GAGGCGGTGG TGGCGGCGGT 
GGAGTGGGCG TAGGCGGTAT GCCGAGCGGC GGAGGGGGCG GTGGCGTGGG GAACGCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dco ( NM_079863.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524602.1 Drosophila melanogaster discs overgrown (dco), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06727
Clone ID Related Accession (Same CDS sequence)	NM_170535.2 , NM_079863.3 , NM_001276203.1 , NM_001369991.1 , NM_170536.3
Accession Version	NM_079863.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	discs overgrown, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079863.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCTGC GCGTGGGTAA CAAATATCGC CTGGGCCGCA AGATAGGATC GGGATCGTTC 
GGCGACATCT ACCTGGGCAC CACGATCAAC ACTGGCGAGG AGGTGGCCAT CAAGCTGGAG 
TGCATCCGCA CCAAACACCC CCAGCTGCAC ATCGAGTCAA AGTTCTACAA GACGATGCAG 
GGTGGCATAG GCATACCCCG CATAATCTGG TGCGGCAGCG AGGGCGACTA CAATGTGATG 
GTGATGGAGC TACTCGGACC CTCGCTGGAG GACCTCTTCA ACTTTTGTTC ACGCCGCTTT 
TCGTTGAAGA CGGTTCTGCT GCTGGCGGAC CAGATGATCT CCCGCATCGA TTACATACAC 
TCGCGGGACT TCATCCATCG CGACATCAAG CCGGATAACT TCCTCATGGG TCTTGGCAAA 
AAGGGCAACC TGGTGTACAT CATTGACTTT GGCCTGGCCA AGAAATTCCG CGATGCCCGG 
TCGCTGAAGC ACATTCCCTA TCGGGAAAAC AAGAACCTCA CGGGCACTGC CCGCTATGCC 
TCCATCAACA CACATTTGGG CATTGAGCAA TCGCGTCGTG ACGACCTGGA GTCCCTGGGC 
TACGTCCTAA TGTACTTCAA TCTGGGCGCC TTGCCCTGGC AGGGCTTAAA GGCAGCCAAC 
AAGAGGCAAA AGTACGAGAG GATCTCGGAG AAGAAGCTGT CCACCTCGAT TGTGGTGCTG 
TGCAAGGGCT TCCCCAGCGA GTTCGTCAAC TATCTGAACT TCTGTCGCCA GATGCATTTC 
GACCAGCGTC CCGATTACTG CCACCTGCGC AAACTCTTCC GCAACTTGTT CCACCGTTTG 
GGCTTCACTT ACGACTATGT GTTTGACTGG AACCTGCTTA AGTTTGGCGG ACCTCGCAAT 
CCCCAGGCGA TTCAGCAGGC GCAGGACGGA GCGGACGGCC AGGCGGGTCA TGATGCGGTG 
GCCGCAGCAG CGGCGGTGGC AGCAGCGGCA GCCGCCTCCT CGCATCAACA ACAGCAGCAC 
AAGGTCAATG CGGCGCTGGG TGGCGGAGGA GGCAGTGCAG CGCAACAGCA ACTCCAGGGC 
GGCCAAACGC TGGCGATGCT GGGCGGCAAT GGAGGCGGTA ACGGCAGCCA ACTGATCGGC 
GGCAACGGAC TCAACATGGA CGACTCGATG GCGGCCACCA ACTCGTCGAG ACCGCCGTAC 
GACACGCCGG AGCGTCGGCC TTCGATACGG ATGCGGCAGG GAGGCGGTGG TGGCGGCGGT 
GGAGTGGGCG TAGGCGGTAT GCCGAGCGGC GGAGGGGGCG GTGGCGTGGG GAACGCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524602.1
CDS	1002..2324
Translation	MELRVGNKYRLGRKIGSGSFGDIYLGTTINTGEEVAIKLECIRTKHPQLHIESKFYKTMQGGIGIPRIIWCGSEGDYNVMVMELLGPSLEDLFNFCSRRFSLKTVLLLADQMISRIDYIHSRDFIHRDIKPDNFLMGLGKKGNLVYIIDFGLAKKFRDARSLKHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGALPWQGLKAANKRQKYERISEKKLSTSIVVLCKGFPSEFVNYLNFCRQMHFDQRPDYCHLRKLFRNLFHRLGFTYDYVFDWNLLKFGGPRNPQAIQQAQDGADGQAGHDAVAAAAAVAAAAAASSHQQQQHKVNAALGGGGGSAAQQQLQGGQTLAMLGGNGGGNGSQLIGGNGLNMDDSMAATNSSRPPYDTPERRPSIRMRQGGGGGGGGVGVGGMPSGGGGGGVGNAK

Target ORF information:

RefSeq Version	NM_079863.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster discs overgrown (dco), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079863.3

ORF Insert Sequence:

ATGGAGCTGC GCGTGGGTAA CAAATATCGC CTGGGCCGCA AGATAGGATC GGGATCGTTC 
GGCGACATCT ACCTGGGCAC CACGATCAAC ACTGGCGAGG AGGTGGCCAT CAAGCTGGAG 
TGCATCCGCA CCAAACACCC CCAGCTGCAC ATCGAGTCAA AGTTCTACAA GACGATGCAG 
GGTGGCATAG GCATACCCCG CATAATCTGG TGCGGCAGCG AGGGCGACTA CAATGTGATG 
GTGATGGAGC TACTCGGACC CTCGCTGGAG GACCTCTTCA ACTTTTGTTC ACGCCGCTTT 
TCGTTGAAGA CGGTTCTGCT GCTGGCGGAC CAGATGATCT CCCGCATCGA TTACATACAC 
TCGCGGGACT TCATCCATCG CGACATCAAG CCGGATAACT TCCTCATGGG TCTTGGCAAA 
AAGGGCAACC TGGTGTACAT CATTGACTTT GGCCTGGCCA AGAAATTCCG CGATGCCCGG 
TCGCTGAAGC ACATTCCCTA TCGGGAAAAC AAGAACCTCA CGGGCACTGC CCGCTATGCC 
TCCATCAACA CACATTTGGG CATTGAGCAA TCGCGTCGTG ACGACCTGGA GTCCCTGGGC 
TACGTCCTAA TGTACTTCAA TCTGGGCGCC TTGCCCTGGC AGGGCTTAAA GGCAGCCAAC 
AAGAGGCAAA AGTACGAGAG GATCTCGGAG AAGAAGCTGT CCACCTCGAT TGTGGTGCTG 
TGCAAGGGCT TCCCCAGCGA GTTCGTCAAC TATCTGAACT TCTGTCGCCA GATGCATTTC 
GACCAGCGTC CCGATTACTG CCACCTGCGC AAACTCTTCC GCAACTTGTT CCACCGTTTG 
GGCTTCACTT ACGACTATGT GTTTGACTGG AACCTGCTTA AGTTTGGCGG ACCTCGCAAT 
CCCCAGGCGA TTCAGCAGGC GCAGGACGGA GCGGACGGCC AGGCGGGTCA TGATGCGGTG 
GCCGCAGCAG CGGCGGTGGC AGCAGCGGCA GCCGCCTCCT CGCATCAACA ACAGCAGCAC 
AAGGTCAATG CGGCGCTGGG TGGCGGAGGA GGCAGTGCAG CGCAACAGCA ACTCCAGGGC 
GGCCAAACGC TGGCGATGCT GGGCGGCAAT GGAGGCGGTA ACGGCAGCCA ACTGATCGGC 
GGCAACGGAC TCAACATGGA CGACTCGATG GCGGCCACCA ACTCGTCGAG ACCGCCGTAC 
GACACGCCGG AGCGTCGGCC TTCGATACGG ATGCGGCAGG GAGGCGGTGG TGGCGGCGGT 
GGAGTGGGCG TAGGCGGTAT GCCGAGCGGC GGAGGGGGCG GTGGCGTGGG GAACGCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dco ( NM_001276203.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263132.1 Drosophila melanogaster discs overgrown (dco), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa06727
Clone ID Related Accession (Same CDS sequence)	NM_170535.2 , NM_079863.3 , NM_001276203.1 , NM_001369991.1 , NM_170536.3
Accession Version	NM_001276203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	discs overgrown, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCTGC GCGTGGGTAA CAAATATCGC CTGGGCCGCA AGATAGGATC GGGATCGTTC 
GGCGACATCT ACCTGGGCAC CACGATCAAC ACTGGCGAGG AGGTGGCCAT CAAGCTGGAG 
TGCATCCGCA CCAAACACCC CCAGCTGCAC ATCGAGTCAA AGTTCTACAA GACGATGCAG 
GGTGGCATAG GCATACCCCG CATAATCTGG TGCGGCAGCG AGGGCGACTA CAATGTGATG 
GTGATGGAGC TACTCGGACC CTCGCTGGAG GACCTCTTCA ACTTTTGTTC ACGCCGCTTT 
TCGTTGAAGA CGGTTCTGCT GCTGGCGGAC CAGATGATCT CCCGCATCGA TTACATACAC 
TCGCGGGACT TCATCCATCG CGACATCAAG CCGGATAACT TCCTCATGGG TCTTGGCAAA 
AAGGGCAACC TGGTGTACAT CATTGACTTT GGCCTGGCCA AGAAATTCCG CGATGCCCGG 
TCGCTGAAGC ACATTCCCTA TCGGGAAAAC AAGAACCTCA CGGGCACTGC CCGCTATGCC 
TCCATCAACA CACATTTGGG CATTGAGCAA TCGCGTCGTG ACGACCTGGA GTCCCTGGGC 
TACGTCCTAA TGTACTTCAA TCTGGGCGCC TTGCCCTGGC AGGGCTTAAA GGCAGCCAAC 
AAGAGGCAAA AGTACGAGAG GATCTCGGAG AAGAAGCTGT CCACCTCGAT TGTGGTGCTG 
TGCAAGGGCT TCCCCAGCGA GTTCGTCAAC TATCTGAACT TCTGTCGCCA GATGCATTTC 
GACCAGCGTC CCGATTACTG CCACCTGCGC AAACTCTTCC GCAACTTGTT CCACCGTTTG 
GGCTTCACTT ACGACTATGT GTTTGACTGG AACCTGCTTA AGTTTGGCGG ACCTCGCAAT 
CCCCAGGCGA TTCAGCAGGC GCAGGACGGA GCGGACGGCC AGGCGGGTCA TGATGCGGTG 
GCCGCAGCAG CGGCGGTGGC AGCAGCGGCA GCCGCCTCCT CGCATCAACA ACAGCAGCAC 
AAGGTCAATG CGGCGCTGGG TGGCGGAGGA GGCAGTGCAG CGCAACAGCA ACTCCAGGGC 
GGCCAAACGC TGGCGATGCT GGGCGGCAAT GGAGGCGGTA ACGGCAGCCA ACTGATCGGC 
GGCAACGGAC TCAACATGGA CGACTCGATG GCGGCCACCA ACTCGTCGAG ACCGCCGTAC 
GACACGCCGG AGCGTCGGCC TTCGATACGG ATGCGGCAGG GAGGCGGTGG TGGCGGCGGT 
GGAGTGGGCG TAGGCGGTAT GCCGAGCGGC GGAGGGGGCG GTGGCGTGGG GAACGCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263132.1
CDS	390..1712
Translation	MELRVGNKYRLGRKIGSGSFGDIYLGTTINTGEEVAIKLECIRTKHPQLHIESKFYKTMQGGIGIPRIIWCGSEGDYNVMVMELLGPSLEDLFNFCSRRFSLKTVLLLADQMISRIDYIHSRDFIHRDIKPDNFLMGLGKKGNLVYIIDFGLAKKFRDARSLKHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGALPWQGLKAANKRQKYERISEKKLSTSIVVLCKGFPSEFVNYLNFCRQMHFDQRPDYCHLRKLFRNLFHRLGFTYDYVFDWNLLKFGGPRNPQAIQQAQDGADGQAGHDAVAAAAAVAAAAAASSHQQQQHKVNAALGGGGGSAAQQQLQGGQTLAMLGGNGGGNGSQLIGGNGLNMDDSMAATNSSRPPYDTPERRPSIRMRQGGGGGGGGVGVGGMPSGGGGGGVGNAK

Target ORF information:

RefSeq Version	NM_001276203.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster discs overgrown (dco), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276203.1

ORF Insert Sequence:

ATGGAGCTGC GCGTGGGTAA CAAATATCGC CTGGGCCGCA AGATAGGATC GGGATCGTTC 
GGCGACATCT ACCTGGGCAC CACGATCAAC ACTGGCGAGG AGGTGGCCAT CAAGCTGGAG 
TGCATCCGCA CCAAACACCC CCAGCTGCAC ATCGAGTCAA AGTTCTACAA GACGATGCAG 
GGTGGCATAG GCATACCCCG CATAATCTGG TGCGGCAGCG AGGGCGACTA CAATGTGATG 
GTGATGGAGC TACTCGGACC CTCGCTGGAG GACCTCTTCA ACTTTTGTTC ACGCCGCTTT 
TCGTTGAAGA CGGTTCTGCT GCTGGCGGAC CAGATGATCT CCCGCATCGA TTACATACAC 
TCGCGGGACT TCATCCATCG CGACATCAAG CCGGATAACT TCCTCATGGG TCTTGGCAAA 
AAGGGCAACC TGGTGTACAT CATTGACTTT GGCCTGGCCA AGAAATTCCG CGATGCCCGG 
TCGCTGAAGC ACATTCCCTA TCGGGAAAAC AAGAACCTCA CGGGCACTGC CCGCTATGCC 
TCCATCAACA CACATTTGGG CATTGAGCAA TCGCGTCGTG ACGACCTGGA GTCCCTGGGC 
TACGTCCTAA TGTACTTCAA TCTGGGCGCC TTGCCCTGGC AGGGCTTAAA GGCAGCCAAC 
AAGAGGCAAA AGTACGAGAG GATCTCGGAG AAGAAGCTGT CCACCTCGAT TGTGGTGCTG 
TGCAAGGGCT TCCCCAGCGA GTTCGTCAAC TATCTGAACT TCTGTCGCCA GATGCATTTC 
GACCAGCGTC CCGATTACTG CCACCTGCGC AAACTCTTCC GCAACTTGTT CCACCGTTTG 
GGCTTCACTT ACGACTATGT GTTTGACTGG AACCTGCTTA AGTTTGGCGG ACCTCGCAAT 
CCCCAGGCGA TTCAGCAGGC GCAGGACGGA GCGGACGGCC AGGCGGGTCA TGATGCGGTG 
GCCGCAGCAG CGGCGGTGGC AGCAGCGGCA GCCGCCTCCT CGCATCAACA ACAGCAGCAC 
AAGGTCAATG CGGCGCTGGG TGGCGGAGGA GGCAGTGCAG CGCAACAGCA ACTCCAGGGC 
GGCCAAACGC TGGCGATGCT GGGCGGCAAT GGAGGCGGTA ACGGCAGCCA ACTGATCGGC 
GGCAACGGAC TCAACATGGA CGACTCGATG GCGGCCACCA ACTCGTCGAG ACCGCCGTAC 
GACACGCCGG AGCGTCGGCC TTCGATACGG ATGCGGCAGG GAGGCGGTGG TGGCGGCGGT 
GGAGTGGGCG TAGGCGGTAT GCCGAGCGGC GGAGGGGGCG GTGGCGTGGG GAACGCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dco ( NM_001369991.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356928.1 Drosophila melanogaster discs overgrown (dco), transcript variant E, mRNA.

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CloneID	OFa06727
Clone ID Related Accession (Same CDS sequence)	NM_170535.2 , NM_079863.3 , NM_001276203.1 , NM_001369991.1 , NM_170536.3
Accession Version	NM_001369991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	discs overgrown, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCTGC GCGTGGGTAA CAAATATCGC CTGGGCCGCA AGATAGGATC GGGATCGTTC 
GGCGACATCT ACCTGGGCAC CACGATCAAC ACTGGCGAGG AGGTGGCCAT CAAGCTGGAG 
TGCATCCGCA CCAAACACCC CCAGCTGCAC ATCGAGTCAA AGTTCTACAA GACGATGCAG 
GGTGGCATAG GCATACCCCG CATAATCTGG TGCGGCAGCG AGGGCGACTA CAATGTGATG 
GTGATGGAGC TACTCGGACC CTCGCTGGAG GACCTCTTCA ACTTTTGTTC ACGCCGCTTT 
TCGTTGAAGA CGGTTCTGCT GCTGGCGGAC CAGATGATCT CCCGCATCGA TTACATACAC 
TCGCGGGACT TCATCCATCG CGACATCAAG CCGGATAACT TCCTCATGGG TCTTGGCAAA 
AAGGGCAACC TGGTGTACAT CATTGACTTT GGCCTGGCCA AGAAATTCCG CGATGCCCGG 
TCGCTGAAGC ACATTCCCTA TCGGGAAAAC AAGAACCTCA CGGGCACTGC CCGCTATGCC 
TCCATCAACA CACATTTGGG CATTGAGCAA TCGCGTCGTG ACGACCTGGA GTCCCTGGGC 
TACGTCCTAA TGTACTTCAA TCTGGGCGCC TTGCCCTGGC AGGGCTTAAA GGCAGCCAAC 
AAGAGGCAAA AGTACGAGAG GATCTCGGAG AAGAAGCTGT CCACCTCGAT TGTGGTGCTG 
TGCAAGGGCT TCCCCAGCGA GTTCGTCAAC TATCTGAACT TCTGTCGCCA GATGCATTTC 
GACCAGCGTC CCGATTACTG CCACCTGCGC AAACTCTTCC GCAACTTGTT CCACCGTTTG 
GGCTTCACTT ACGACTATGT GTTTGACTGG AACCTGCTTA AGTTTGGCGG ACCTCGCAAT 
CCCCAGGCGA TTCAGCAGGC GCAGGACGGA GCGGACGGCC AGGCGGGTCA TGATGCGGTG 
GCCGCAGCAG CGGCGGTGGC AGCAGCGGCA GCCGCCTCCT CGCATCAACA ACAGCAGCAC 
AAGGTCAATG CGGCGCTGGG TGGCGGAGGA GGCAGTGCAG CGCAACAGCA ACTCCAGGGC 
GGCCAAACGC TGGCGATGCT GGGCGGCAAT GGAGGCGGTA ACGGCAGCCA ACTGATCGGC 
GGCAACGGAC TCAACATGGA CGACTCGATG GCGGCCACCA ACTCGTCGAG ACCGCCGTAC 
GACACGCCGG AGCGTCGGCC TTCGATACGG ATGCGGCAGG GAGGCGGTGG TGGCGGCGGT 
GGAGTGGGCG TAGGCGGTAT GCCGAGCGGC GGAGGGGGCG GTGGCGTGGG GAACGCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356928.1
CDS	565..1887
Translation	MELRVGNKYRLGRKIGSGSFGDIYLGTTINTGEEVAIKLECIRTKHPQLHIESKFYKTMQGGIGIPRIIWCGSEGDYNVMVMELLGPSLEDLFNFCSRRFSLKTVLLLADQMISRIDYIHSRDFIHRDIKPDNFLMGLGKKGNLVYIIDFGLAKKFRDARSLKHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGALPWQGLKAANKRQKYERISEKKLSTSIVVLCKGFPSEFVNYLNFCRQMHFDQRPDYCHLRKLFRNLFHRLGFTYDYVFDWNLLKFGGPRNPQAIQQAQDGADGQAGHDAVAAAAAVAAAAAASSHQQQQHKVNAALGGGGGSAAQQQLQGGQTLAMLGGNGGGNGSQLIGGNGLNMDDSMAATNSSRPPYDTPERRPSIRMRQGGGGGGGGVGVGGMPSGGGGGGVGNAK

Target ORF information:

RefSeq Version	NM_001369991.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster discs overgrown (dco), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369991.1

ORF Insert Sequence:

ATGGAGCTGC GCGTGGGTAA CAAATATCGC CTGGGCCGCA AGATAGGATC GGGATCGTTC 
GGCGACATCT ACCTGGGCAC CACGATCAAC ACTGGCGAGG AGGTGGCCAT CAAGCTGGAG 
TGCATCCGCA CCAAACACCC CCAGCTGCAC ATCGAGTCAA AGTTCTACAA GACGATGCAG 
GGTGGCATAG GCATACCCCG CATAATCTGG TGCGGCAGCG AGGGCGACTA CAATGTGATG 
GTGATGGAGC TACTCGGACC CTCGCTGGAG GACCTCTTCA ACTTTTGTTC ACGCCGCTTT 
TCGTTGAAGA CGGTTCTGCT GCTGGCGGAC CAGATGATCT CCCGCATCGA TTACATACAC 
TCGCGGGACT TCATCCATCG CGACATCAAG CCGGATAACT TCCTCATGGG TCTTGGCAAA 
AAGGGCAACC TGGTGTACAT CATTGACTTT GGCCTGGCCA AGAAATTCCG CGATGCCCGG 
TCGCTGAAGC ACATTCCCTA TCGGGAAAAC AAGAACCTCA CGGGCACTGC CCGCTATGCC 
TCCATCAACA CACATTTGGG CATTGAGCAA TCGCGTCGTG ACGACCTGGA GTCCCTGGGC 
TACGTCCTAA TGTACTTCAA TCTGGGCGCC TTGCCCTGGC AGGGCTTAAA GGCAGCCAAC 
AAGAGGCAAA AGTACGAGAG GATCTCGGAG AAGAAGCTGT CCACCTCGAT TGTGGTGCTG 
TGCAAGGGCT TCCCCAGCGA GTTCGTCAAC TATCTGAACT TCTGTCGCCA GATGCATTTC 
GACCAGCGTC CCGATTACTG CCACCTGCGC AAACTCTTCC GCAACTTGTT CCACCGTTTG 
GGCTTCACTT ACGACTATGT GTTTGACTGG AACCTGCTTA AGTTTGGCGG ACCTCGCAAT 
CCCCAGGCGA TTCAGCAGGC GCAGGACGGA GCGGACGGCC AGGCGGGTCA TGATGCGGTG 
GCCGCAGCAG CGGCGGTGGC AGCAGCGGCA GCCGCCTCCT CGCATCAACA ACAGCAGCAC 
AAGGTCAATG CGGCGCTGGG TGGCGGAGGA GGCAGTGCAG CGCAACAGCA ACTCCAGGGC 
GGCCAAACGC TGGCGATGCT GGGCGGCAAT GGAGGCGGTA ACGGCAGCCA ACTGATCGGC 
GGCAACGGAC TCAACATGGA CGACTCGATG GCGGCCACCA ACTCGTCGAG ACCGCCGTAC 
GACACGCCGG AGCGTCGGCC TTCGATACGG ATGCGGCAGG GAGGCGGTGG TGGCGGCGGT 
GGAGTGGGCG TAGGCGGTAT GCCGAGCGGC GGAGGGGGCG GTGGCGTGGG GAACGCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dco ( NM_170536.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733415.1 Drosophila melanogaster discs overgrown (dco), transcript variant B, mRNA.

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CloneID	OFa06727
Clone ID Related Accession (Same CDS sequence)	NM_170535.2 , NM_079863.3 , NM_001276203.1 , NM_001369991.1 , NM_170536.3
Accession Version	NM_170536.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	discs overgrown, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170536.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCTGC GCGTGGGTAA CAAATATCGC CTGGGCCGCA AGATAGGATC GGGATCGTTC 
GGCGACATCT ACCTGGGCAC CACGATCAAC ACTGGCGAGG AGGTGGCCAT CAAGCTGGAG 
TGCATCCGCA CCAAACACCC CCAGCTGCAC ATCGAGTCAA AGTTCTACAA GACGATGCAG 
GGTGGCATAG GCATACCCCG CATAATCTGG TGCGGCAGCG AGGGCGACTA CAATGTGATG 
GTGATGGAGC TACTCGGACC CTCGCTGGAG GACCTCTTCA ACTTTTGTTC ACGCCGCTTT 
TCGTTGAAGA CGGTTCTGCT GCTGGCGGAC CAGATGATCT CCCGCATCGA TTACATACAC 
TCGCGGGACT TCATCCATCG CGACATCAAG CCGGATAACT TCCTCATGGG TCTTGGCAAA 
AAGGGCAACC TGGTGTACAT CATTGACTTT GGCCTGGCCA AGAAATTCCG CGATGCCCGG 
TCGCTGAAGC ACATTCCCTA TCGGGAAAAC AAGAACCTCA CGGGCACTGC CCGCTATGCC 
TCCATCAACA CACATTTGGG CATTGAGCAA TCGCGTCGTG ACGACCTGGA GTCCCTGGGC 
TACGTCCTAA TGTACTTCAA TCTGGGCGCC TTGCCCTGGC AGGGCTTAAA GGCAGCCAAC 
AAGAGGCAAA AGTACGAGAG GATCTCGGAG AAGAAGCTGT CCACCTCGAT TGTGGTGCTG 
TGCAAGGGCT TCCCCAGCGA GTTCGTCAAC TATCTGAACT TCTGTCGCCA GATGCATTTC 
GACCAGCGTC CCGATTACTG CCACCTGCGC AAACTCTTCC GCAACTTGTT CCACCGTTTG 
GGCTTCACTT ACGACTATGT GTTTGACTGG AACCTGCTTA AGTTTGGCGG ACCTCGCAAT 
CCCCAGGCGA TTCAGCAGGC GCAGGACGGA GCGGACGGCC AGGCGGGTCA TGATGCGGTG 
GCCGCAGCAG CGGCGGTGGC AGCAGCGGCA GCCGCCTCCT CGCATCAACA ACAGCAGCAC 
AAGGTCAATG CGGCGCTGGG TGGCGGAGGA GGCAGTGCAG CGCAACAGCA ACTCCAGGGC 
GGCCAAACGC TGGCGATGCT GGGCGGCAAT GGAGGCGGTA ACGGCAGCCA ACTGATCGGC 
GGCAACGGAC TCAACATGGA CGACTCGATG GCGGCCACCA ACTCGTCGAG ACCGCCGTAC 
GACACGCCGG AGCGTCGGCC TTCGATACGG ATGCGGCAGG GAGGCGGTGG TGGCGGCGGT 
GGAGTGGGCG TAGGCGGTAT GCCGAGCGGC GGAGGGGGCG GTGGCGTGGG GAACGCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733415.1
CDS	397..1719
Translation	MELRVGNKYRLGRKIGSGSFGDIYLGTTINTGEEVAIKLECIRTKHPQLHIESKFYKTMQGGIGIPRIIWCGSEGDYNVMVMELLGPSLEDLFNFCSRRFSLKTVLLLADQMISRIDYIHSRDFIHRDIKPDNFLMGLGKKGNLVYIIDFGLAKKFRDARSLKHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGALPWQGLKAANKRQKYERISEKKLSTSIVVLCKGFPSEFVNYLNFCRQMHFDQRPDYCHLRKLFRNLFHRLGFTYDYVFDWNLLKFGGPRNPQAIQQAQDGADGQAGHDAVAAAAAVAAAAAASSHQQQQHKVNAALGGGGGSAAQQQLQGGQTLAMLGGNGGGNGSQLIGGNGLNMDDSMAATNSSRPPYDTPERRPSIRMRQGGGGGGGGVGVGGMPSGGGGGGVGNAK

Target ORF information:

RefSeq Version	NM_170536.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster discs overgrown (dco), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170536.3

ORF Insert Sequence:

ATGGAGCTGC GCGTGGGTAA CAAATATCGC CTGGGCCGCA AGATAGGATC GGGATCGTTC 
GGCGACATCT ACCTGGGCAC CACGATCAAC ACTGGCGAGG AGGTGGCCAT CAAGCTGGAG 
TGCATCCGCA CCAAACACCC CCAGCTGCAC ATCGAGTCAA AGTTCTACAA GACGATGCAG 
GGTGGCATAG GCATACCCCG CATAATCTGG TGCGGCAGCG AGGGCGACTA CAATGTGATG 
GTGATGGAGC TACTCGGACC CTCGCTGGAG GACCTCTTCA ACTTTTGTTC ACGCCGCTTT 
TCGTTGAAGA CGGTTCTGCT GCTGGCGGAC CAGATGATCT CCCGCATCGA TTACATACAC 
TCGCGGGACT TCATCCATCG CGACATCAAG CCGGATAACT TCCTCATGGG TCTTGGCAAA 
AAGGGCAACC TGGTGTACAT CATTGACTTT GGCCTGGCCA AGAAATTCCG CGATGCCCGG 
TCGCTGAAGC ACATTCCCTA TCGGGAAAAC AAGAACCTCA CGGGCACTGC CCGCTATGCC 
TCCATCAACA CACATTTGGG CATTGAGCAA TCGCGTCGTG ACGACCTGGA GTCCCTGGGC 
TACGTCCTAA TGTACTTCAA TCTGGGCGCC TTGCCCTGGC AGGGCTTAAA GGCAGCCAAC 
AAGAGGCAAA AGTACGAGAG GATCTCGGAG AAGAAGCTGT CCACCTCGAT TGTGGTGCTG 
TGCAAGGGCT TCCCCAGCGA GTTCGTCAAC TATCTGAACT TCTGTCGCCA GATGCATTTC 
GACCAGCGTC CCGATTACTG CCACCTGCGC AAACTCTTCC GCAACTTGTT CCACCGTTTG 
GGCTTCACTT ACGACTATGT GTTTGACTGG AACCTGCTTA AGTTTGGCGG ACCTCGCAAT 
CCCCAGGCGA TTCAGCAGGC GCAGGACGGA GCGGACGGCC AGGCGGGTCA TGATGCGGTG 
GCCGCAGCAG CGGCGGTGGC AGCAGCGGCA GCCGCCTCCT CGCATCAACA ACAGCAGCAC 
AAGGTCAATG CGGCGCTGGG TGGCGGAGGA GGCAGTGCAG CGCAACAGCA ACTCCAGGGC 
GGCCAAACGC TGGCGATGCT GGGCGGCAAT GGAGGCGGTA ACGGCAGCCA ACTGATCGGC 
GGCAACGGAC TCAACATGGA CGACTCGATG GCGGCCACCA ACTCGTCGAG ACCGCCGTAC 
GACACGCCGG AGCGTCGGCC TTCGATACGG ATGCGGCAGG GAGGCGGTGG TGGCGGCGGT 
GGAGTGGGCG TAGGCGGTAT GCCGAGCGGC GGAGGGGGCG GTGGCGTGGG GAACGCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dco cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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