CanA1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CanA1
Entrez Gene ID	43670
Full Name	Calcineurin A1
Synonyms	CAN A1/PP2B,CG1455,CNA1,Can,Cana1,CnA,CnnA21EF,D14,DmCanA1-100B,Dmel\CG1455,PP2B,PP2B 21EF,PpD14,canA,canA1,pMY4
General protein information	Preferred Names Calcineurin A1 Names CG1455 gene product from transcript CG1455-RD CG1455-PA CG1455-PB CG1455-PD CanA1-PA CanA1-PB CanA1-PD calcineurin calcineurin A calcineurin A1 protein phosphatase D14 protein phosphatase from PCR fragment D14
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100B1-100B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079861.4	NP_524600.3	calcineurin A1, isoform A
NM_001260449.2	NP_001247378.2	calcineurin A1, isoform D
NM_001260448.2	NP_001247377.1	calcineurin A1, isoform B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ppp3cb	NP_058738.1
Drosophila melanogaster (fruit fly)	CanA1	NP_001247378.2
Saccharomyces cerevisiae (baker's yeast)	CMP2	NP_013655.1
Mus musculus (house mouse)	Ppp3cb	NP_032940.1
Homo sapiens (human)	PPP3CB	NP_001135825.1
	PPP3CB	XP_001101616.1
Canis lupus familiaris (dog)	PPP3CB	XP_005619089.1
Bos taurus (cattle)	PPP3CB	NP_001092684.1
Pan troglodytes (chimpanzee)	PPP3CB	XP_001142940.1
Gallus gallus (chicken)	PPP3CB	NP_001025511.1
Xenopus tropicalis (tropical clawed frog)	ppp3cc	NP_001090771.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

upregulation of Nebula/DSCR1 is neuroprotective in the presence of APP upregulation and provides evidence for calcineurin inhibition as a novel target for therapeutic intervention in preventing axonal transport impairments associated with AD.
Title: Nebula/DSCR1 upregulation delays neurodegeneration and protects against APP-induced axonal transport defects by restoring calcineurin and GSK-3? signaling.

results indicate a role for Cn and calcium-dependent signal transduction in sleep and memory regulation.
Title: Pan-neuronal knockdown of calcineurin reduces sleep in the fruit fly, Drosophila melanogaster.

calcineurin, upon activation by Ca(2+)-calmodulin, cooperates with other factors to negatively regulate Egf receptor signaling at the level of sprouty and the GTPase-activating protein Gap1
Title: The Ca(2+)-calmodulin-activated protein phosphatase calcineurin negatively regulates EGF receptor signaling in Drosophila development.

The following CanA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CanA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06723	NM_079861.4 Latest version!	Drosophila melanogaster calcineurin A1, transcript variant A (CanA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa06723	NM_001260449.2 Latest version!	Drosophila melanogaster calcineurin A1, transcript variant D (CanA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa07857	NM_001260448.2 Latest version!	Drosophila melanogaster calcineurin A1, transcript variant B (CanA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CanA1 ( NM_079861.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524600.3 Drosophila melanogaster calcineurin A1, transcript variant A (CanA1), mRNA.

Price & Availability↑

CloneID	OFa06723
Clone ID Related Accession (Same CDS sequence)	NM_079861.4 , NM_001260449.2
Accession Version	NM_079861.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	calcineurin A1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079861.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGCGA CCTCCACGCG AAGTTCGGTG ACGAGGAAAT CATCGCTATC AAAAAGTTCT 
AGTTCAGACA AATCGGCAAA GTCCTCGTCG AATTCCTCGA AATCTCCGAC AGCCGCCAGT 
GGAAACAAAC AGAAAATGCA GTACACAAAA ACCAGGGAGC GCATGGTCGA TGATGTTCCA 
CTTCCGCCCA CCCACAAGCT GACCATGTCG GAGGTATACG ACGATCCGAA GACCGGAAAA 
CCCAACTTCG ACGCCCTGCG CCAGCACTTT CTGCTGGAGG GGCGGATCGA GGAGGCGGTG 
GCCCTGAGGA TCATCACCGA AGGAGCCGCC CTGCTGCGCG AGGAGAAGAA CATGATCGAC 
GTGGAGGCAC CCATTACCGT GTGCGGTGAC ATCCACGGGC AGTTCTTCGA TCTCGTAAAG 
CTCTTCGAAG TGGGCGGCCC GCCGGCCACC ACTAGGTACC TTTTTCTAGG CGACTACGTG 
GACAGGGGAT ACTTCAGTAT TGAGTGCGTT CTCTACCTGT GGTCCCTGAA AATAACCTAC 
CCCACCACGC TTTCCCTGCT GCGAGGAAAT CACGAGTGCA GGCATCTCAC AGAGTACTTT 
ACCTTCAAGC AGGAATGCAT CATCAAGTAT TCGGAAAGTA TTTACGACGC CTGCATGGAA 
GCGTTCGACT GCCTGCCCCT CGCCGCCCTC CTCAACCAGC AGTTCCTCTG TATTCACGGC 
GGTCTGTCGC CCGAGATTTT CACACTCGAC GACATCAAGA CGCTGAATCG CTTCAGGGAA 
CCACCGGCCT ACGGGCCCAT GTGCGACCTG CTCTGGTCCG ATCCTCTGGA GGACTTTGGC 
AACGAGAAGA CCAACGAGTT CTTCTCGCAC AACTCGGTGC GCGGGTGCTC CTACTTCTTC 
AGCTACTCGG CCTGCTGTGA GTTTCTGCAG AAGAACAACC TGCTGTCGAT AGTCAGGGCG 
CACGAGGCGC AGGATGCGGG GTATCGCATG TATCGGAAGA ACCAGGTTAC GGGCTTCCCC 
TCGCTGATCA CCATATTCTC CGCCCCCAAT TATCTGGATG TTTACAATAA CAAGGCAGCT 
GTGCTCAAGT ACGAGAACAA CGTGATGAAT ATCCGCCAGT TCAACTGCTC GCCACATCCC 
TACTGGCTAC CCAACTTCAT GGACGTCTTC ACATGGTCGC TGCCCTTCGT GGGCGAAAAG 
GTAACCGAGA TGCTAGTCAA CATCCTGAAC ATTTGCTCAG ACGATGAACT GGTGGCCGGA 
CCCGATGACG AGCTCGAGGA AGAGCTACGC AAGAAAATTG TGCTCGTGCC GGCGAACGCG 
AGCAATAATA ACAATAACAA TAATACGCCC AGTAAGCCAG CTTCCATGTC GGCGCTGCGC 
AAAGAGATAA TTCGGAATAA GATTCGGGCC ATTGGCAAGA TGTCGCGCGT CTTCTCCATC 
CTCAGGGAGG AATCCGAGAG CGTGCTCCAG CTGAAGGGTC TGACGCCCAC GGGCGCCCTG 
CCAGTGGGCG CTTTGTCCGG CGGAAGGGAT TCCCTCAAGG AGGCTCTGCA AGGACTGACC 
GCATCCTCGC ACATCCACTC CTTTGCGGAG GCCAAGGGCC TGGATGCGGT CAACGAACGG 
ATGCCGCCCC GTCGTCCTCT TTTGATGAGT GCCAGCAGCA GCAGCATCAC CACGGTCACA 
AGGAGCAGCA GCAGCAGCAG CAACAACAAC AACAATAACA GCAACACCAG CAGCACCACG 
ACGACAAAGG ACATCAGCAA CACCAGCAGT AATGATACCG CAACCGTAAC CAAAACGAGC 
AGGACGACTG TGAAATCGGC CACCACCAGT AATGTGCGGG CAGGATTCAC CGCCAAGAAG 
TTCCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524600.3
CDS	330..2198
Translation	MSATSTRSSVTRKSSLSKSSSSDKSAKSSSNSSKSPTAASGNKQKMQYTKTRERMVDDVPLPPTHKLTMSEVYDDPKTGKPNFDALRQHFLLEGRIEEAVALRIITEGAALLREEKNMIDVEAPITVCGDIHGQFFDLVKLFEVGGPPATTRYLFLGDYVDRGYFSIECVLYLWSLKITYPTTLSLLRGNHECRHLTEYFTFKQECIIKYSESIYDACMEAFDCLPLAALLNQQFLCIHGGLSPEIFTLDDIKTLNRFREPPAYGPMCDLLWSDPLEDFGNEKTNEFFSHNSVRGCSYFFSYSACCEFLQKNNLLSIVRAHEAQDAGYRMYRKNQVTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNILNICSDDELVAGPDDELEEELRKKIVLVPANASNNNNNNNTPSKPASMSALRKEIIRNKIRAIGKMSRVFSILREESESVLQLKGLTPTGALPVGALSGGRDSLKEALQGLTASSHIHSFAEAKGLDAVNERMPPRRPLLMSASSSSITTVTRSSSSSSNNNNNNSNTSSTTTTKDISNTSSNDTATVTKTSRTTVKSATTSNVRAGFTAKKFP

Target ORF information:

RefSeq Version	NM_079861.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster calcineurin A1, transcript variant A (CanA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079861.4

ORF Insert Sequence:

ATGTCGGCGA CCTCCACGCG AAGTTCGGTG ACGAGGAAAT CATCGCTATC AAAAAGTTCT 
AGTTCAGACA AATCGGCAAA GTCCTCGTCG AATTCCTCGA AATCTCCGAC AGCCGCCAGT 
GGAAACAAAC AGAAAATGCA GTACACAAAA ACCAGGGAGC GCATGGTCGA TGATGTTCCA 
CTTCCGCCCA CCCACAAGCT GACCATGTCG GAGGTATACG ACGATCCGAA GACCGGAAAA 
CCCAACTTCG ACGCCCTGCG CCAGCACTTT CTGCTGGAGG GGCGGATCGA GGAGGCGGTG 
GCCCTGAGGA TCATCACCGA AGGAGCCGCC CTGCTGCGCG AGGAGAAGAA CATGATCGAC 
GTGGAGGCAC CCATTACCGT GTGCGGTGAC ATCCACGGGC AGTTCTTCGA TCTCGTAAAG 
CTCTTCGAAG TGGGCGGCCC GCCGGCCACC ACTAGGTACC TTTTTCTAGG CGACTACGTG 
GACAGGGGAT ACTTCAGTAT TGAGTGCGTT CTCTACCTGT GGTCCCTGAA AATAACCTAC 
CCCACCACGC TTTCCCTGCT GCGAGGAAAT CACGAGTGCA GGCATCTCAC AGAGTACTTT 
ACCTTCAAGC AGGAATGCAT CATCAAGTAT TCGGAAAGTA TTTACGACGC CTGCATGGAA 
GCGTTCGACT GCCTGCCCCT CGCCGCCCTC CTCAACCAGC AGTTCCTCTG TATTCACGGC 
GGTCTGTCGC CCGAGATTTT CACACTCGAC GACATCAAGA CGCTGAATCG CTTCAGGGAA 
CCACCGGCCT ACGGGCCCAT GTGCGACCTG CTCTGGTCCG ATCCTCTGGA GGACTTTGGC 
AACGAGAAGA CCAACGAGTT CTTCTCGCAC AACTCGGTGC GCGGGTGCTC CTACTTCTTC 
AGCTACTCGG CCTGCTGTGA GTTTCTGCAG AAGAACAACC TGCTGTCGAT AGTCAGGGCG 
CACGAGGCGC AGGATGCGGG GTATCGCATG TATCGGAAGA ACCAGGTTAC GGGCTTCCCC 
TCGCTGATCA CCATATTCTC CGCCCCCAAT TATCTGGATG TTTACAATAA CAAGGCAGCT 
GTGCTCAAGT ACGAGAACAA CGTGATGAAT ATCCGCCAGT TCAACTGCTC GCCACATCCC 
TACTGGCTAC CCAACTTCAT GGACGTCTTC ACATGGTCGC TGCCCTTCGT GGGCGAAAAG 
GTAACCGAGA TGCTAGTCAA CATCCTGAAC ATTTGCTCAG ACGATGAACT GGTGGCCGGA 
CCCGATGACG AGCTCGAGGA AGAGCTACGC AAGAAAATTG TGCTCGTGCC GGCGAACGCG 
AGCAATAATA ACAATAACAA TAATACGCCC AGTAAGCCAG CTTCCATGTC GGCGCTGCGC 
AAAGAGATAA TTCGGAATAA GATTCGGGCC ATTGGCAAGA TGTCGCGCGT CTTCTCCATC 
CTCAGGGAGG AATCCGAGAG CGTGCTCCAG CTGAAGGGTC TGACGCCCAC GGGCGCCCTG 
CCAGTGGGCG CTTTGTCCGG CGGAAGGGAT TCCCTCAAGG AGGCTCTGCA AGGACTGACC 
GCATCCTCGC ACATCCACTC CTTTGCGGAG GCCAAGGGCC TGGATGCGGT CAACGAACGG 
ATGCCGCCCC GTCGTCCTCT TTTGATGAGT GCCAGCAGCA GCAGCATCAC CACGGTCACA 
AGGAGCAGCA GCAGCAGCAG CAACAACAAC AACAATAACA GCAACACCAG CAGCACCACG 
ACGACAAAGG ACATCAGCAA CACCAGCAGT AATGATACCG CAACCGTAAC CAAAACGAGC 
AGGACGACTG TGAAATCGGC CACCACCAGT AATGTGCGGG CAGGATTCAC CGCCAAGAAG 
TTCCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CanA1 ( NM_001260449.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247378.2 Drosophila melanogaster calcineurin A1, transcript variant D (CanA1), mRNA.

Price & Availability↑

CloneID	OFa06723
Clone ID Related Accession (Same CDS sequence)	NM_079861.4 , NM_001260449.2
Accession Version	NM_001260449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	calcineurin A1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260449.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGCGA CCTCCACGCG AAGTTCGGTG ACGAGGAAAT CATCGCTATC AAAAAGTTCT 
AGTTCAGACA AATCGGCAAA GTCCTCGTCG AATTCCTCGA AATCTCCGAC AGCCGCCAGT 
GGAAACAAAC AGAAAATGCA GTACACAAAA ACCAGGGAGC GCATGGTCGA TGATGTTCCA 
CTTCCGCCCA CCCACAAGCT GACCATGTCG GAGGTATACG ACGATCCGAA GACCGGAAAA 
CCCAACTTCG ACGCCCTGCG CCAGCACTTT CTGCTGGAGG GGCGGATCGA GGAGGCGGTG 
GCCCTGAGGA TCATCACCGA AGGAGCCGCC CTGCTGCGCG AGGAGAAGAA CATGATCGAC 
GTGGAGGCAC CCATTACCGT GTGCGGTGAC ATCCACGGGC AGTTCTTCGA TCTCGTAAAG 
CTCTTCGAAG TGGGCGGCCC GCCGGCCACC ACTAGGTACC TTTTTCTAGG CGACTACGTG 
GACAGGGGAT ACTTCAGTAT TGAGTGCGTT CTCTACCTGT GGTCCCTGAA AATAACCTAC 
CCCACCACGC TTTCCCTGCT GCGAGGAAAT CACGAGTGCA GGCATCTCAC AGAGTACTTT 
ACCTTCAAGC AGGAATGCAT CATCAAGTAT TCGGAAAGTA TTTACGACGC CTGCATGGAA 
GCGTTCGACT GCCTGCCCCT CGCCGCCCTC CTCAACCAGC AGTTCCTCTG TATTCACGGC 
GGTCTGTCGC CCGAGATTTT CACACTCGAC GACATCAAGA CGCTGAATCG CTTCAGGGAA 
CCACCGGCCT ACGGGCCCAT GTGCGACCTG CTCTGGTCCG ATCCTCTGGA GGACTTTGGC 
AACGAGAAGA CCAACGAGTT CTTCTCGCAC AACTCGGTGC GCGGGTGCTC CTACTTCTTC 
AGCTACTCGG CCTGCTGTGA GTTTCTGCAG AAGAACAACC TGCTGTCGAT AGTCAGGGCG 
CACGAGGCGC AGGATGCGGG GTATCGCATG TATCGGAAGA ACCAGGTTAC GGGCTTCCCC 
TCGCTGATCA CCATATTCTC CGCCCCCAAT TATCTGGATG TTTACAATAA CAAGGCAGCT 
GTGCTCAAGT ACGAGAACAA CGTGATGAAT ATCCGCCAGT TCAACTGCTC GCCACATCCC 
TACTGGCTAC CCAACTTCAT GGACGTCTTC ACATGGTCGC TGCCCTTCGT GGGCGAAAAG 
GTAACCGAGA TGCTAGTCAA CATCCTGAAC ATTTGCTCAG ACGATGAACT GGTGGCCGGA 
CCCGATGACG AGCTCGAGGA AGAGCTACGC AAGAAAATTG TGCTCGTGCC GGCGAACGCG 
AGCAATAATA ACAATAACAA TAATACGCCC AGTAAGCCAG CTTCCATGTC GGCGCTGCGC 
AAAGAGATAA TTCGGAATAA GATTCGGGCC ATTGGCAAGA TGTCGCGCGT CTTCTCCATC 
CTCAGGGAGG AATCCGAGAG CGTGCTCCAG CTGAAGGGTC TGACGCCCAC GGGCGCCCTG 
CCAGTGGGCG CTTTGTCCGG CGGAAGGGAT TCCCTCAAGG AGGCTCTGCA AGGACTGACC 
GCATCCTCGC ACATCCACTC CTTTGCGGAG GCCAAGGGCC TGGATGCGGT CAACGAACGG 
ATGCCGCCCC GTCGTCCTCT TTTGATGAGT GCCAGCAGCA GCAGCATCAC CACGGTCACA 
AGGAGCAGCA GCAGCAGCAG CAACAACAAC AACAATAACA GCAACACCAG CAGCACCACG 
ACGACAAAGG ACATCAGCAA CACCAGCAGT AATGATACCG CAACCGTAAC CAAAACGAGC 
AGGACGACTG TGAAATCGGC CACCACCAGT AATGTGCGGG CAGGATTCAC CGCCAAGAAG 
TTCCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247378.2
CDS	766..2634
Translation	MSATSTRSSVTRKSSLSKSSSSDKSAKSSSNSSKSPTAASGNKQKMQYTKTRERMVDDVPLPPTHKLTMSEVYDDPKTGKPNFDALRQHFLLEGRIEEAVALRIITEGAALLREEKNMIDVEAPITVCGDIHGQFFDLVKLFEVGGPPATTRYLFLGDYVDRGYFSIECVLYLWSLKITYPTTLSLLRGNHECRHLTEYFTFKQECIIKYSESIYDACMEAFDCLPLAALLNQQFLCIHGGLSPEIFTLDDIKTLNRFREPPAYGPMCDLLWSDPLEDFGNEKTNEFFSHNSVRGCSYFFSYSACCEFLQKNNLLSIVRAHEAQDAGYRMYRKNQVTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNILNICSDDELVAGPDDELEEELRKKIVLVPANASNNNNNNNTPSKPASMSALRKEIIRNKIRAIGKMSRVFSILREESESVLQLKGLTPTGALPVGALSGGRDSLKEALQGLTASSHIHSFAEAKGLDAVNERMPPRRPLLMSASSSSITTVTRSSSSSSNNNNNNSNTSSTTTTKDISNTSSNDTATVTKTSRTTVKSATTSNVRAGFTAKKFP

Target ORF information:

RefSeq Version	NM_001260449.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster calcineurin A1, transcript variant D (CanA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260449.2

ORF Insert Sequence:

ATGTCGGCGA CCTCCACGCG AAGTTCGGTG ACGAGGAAAT CATCGCTATC AAAAAGTTCT 
AGTTCAGACA AATCGGCAAA GTCCTCGTCG AATTCCTCGA AATCTCCGAC AGCCGCCAGT 
GGAAACAAAC AGAAAATGCA GTACACAAAA ACCAGGGAGC GCATGGTCGA TGATGTTCCA 
CTTCCGCCCA CCCACAAGCT GACCATGTCG GAGGTATACG ACGATCCGAA GACCGGAAAA 
CCCAACTTCG ACGCCCTGCG CCAGCACTTT CTGCTGGAGG GGCGGATCGA GGAGGCGGTG 
GCCCTGAGGA TCATCACCGA AGGAGCCGCC CTGCTGCGCG AGGAGAAGAA CATGATCGAC 
GTGGAGGCAC CCATTACCGT GTGCGGTGAC ATCCACGGGC AGTTCTTCGA TCTCGTAAAG 
CTCTTCGAAG TGGGCGGCCC GCCGGCCACC ACTAGGTACC TTTTTCTAGG CGACTACGTG 
GACAGGGGAT ACTTCAGTAT TGAGTGCGTT CTCTACCTGT GGTCCCTGAA AATAACCTAC 
CCCACCACGC TTTCCCTGCT GCGAGGAAAT CACGAGTGCA GGCATCTCAC AGAGTACTTT 
ACCTTCAAGC AGGAATGCAT CATCAAGTAT TCGGAAAGTA TTTACGACGC CTGCATGGAA 
GCGTTCGACT GCCTGCCCCT CGCCGCCCTC CTCAACCAGC AGTTCCTCTG TATTCACGGC 
GGTCTGTCGC CCGAGATTTT CACACTCGAC GACATCAAGA CGCTGAATCG CTTCAGGGAA 
CCACCGGCCT ACGGGCCCAT GTGCGACCTG CTCTGGTCCG ATCCTCTGGA GGACTTTGGC 
AACGAGAAGA CCAACGAGTT CTTCTCGCAC AACTCGGTGC GCGGGTGCTC CTACTTCTTC 
AGCTACTCGG CCTGCTGTGA GTTTCTGCAG AAGAACAACC TGCTGTCGAT AGTCAGGGCG 
CACGAGGCGC AGGATGCGGG GTATCGCATG TATCGGAAGA ACCAGGTTAC GGGCTTCCCC 
TCGCTGATCA CCATATTCTC CGCCCCCAAT TATCTGGATG TTTACAATAA CAAGGCAGCT 
GTGCTCAAGT ACGAGAACAA CGTGATGAAT ATCCGCCAGT TCAACTGCTC GCCACATCCC 
TACTGGCTAC CCAACTTCAT GGACGTCTTC ACATGGTCGC TGCCCTTCGT GGGCGAAAAG 
GTAACCGAGA TGCTAGTCAA CATCCTGAAC ATTTGCTCAG ACGATGAACT GGTGGCCGGA 
CCCGATGACG AGCTCGAGGA AGAGCTACGC AAGAAAATTG TGCTCGTGCC GGCGAACGCG 
AGCAATAATA ACAATAACAA TAATACGCCC AGTAAGCCAG CTTCCATGTC GGCGCTGCGC 
AAAGAGATAA TTCGGAATAA GATTCGGGCC ATTGGCAAGA TGTCGCGCGT CTTCTCCATC 
CTCAGGGAGG AATCCGAGAG CGTGCTCCAG CTGAAGGGTC TGACGCCCAC GGGCGCCCTG 
CCAGTGGGCG CTTTGTCCGG CGGAAGGGAT TCCCTCAAGG AGGCTCTGCA AGGACTGACC 
GCATCCTCGC ACATCCACTC CTTTGCGGAG GCCAAGGGCC TGGATGCGGT CAACGAACGG 
ATGCCGCCCC GTCGTCCTCT TTTGATGAGT GCCAGCAGCA GCAGCATCAC CACGGTCACA 
AGGAGCAGCA GCAGCAGCAG CAACAACAAC AACAATAACA GCAACACCAG CAGCACCACG 
ACGACAAAGG ACATCAGCAA CACCAGCAGT AATGATACCG CAACCGTAAC CAAAACGAGC 
AGGACGACTG TGAAATCGGC CACCACCAGT AATGTGCGGG CAGGATTCAC CGCCAAGAAG 
TTCCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CanA1 ( NM_001260448.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247377.1 Drosophila melanogaster calcineurin A1, transcript variant B (CanA1), mRNA.

Price & Availability↑

CloneID	OFa07857
Clone ID Related Accession (Same CDS sequence)	NM_001260448.2
Accession Version	NM_001260448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	calcineurin A1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260448.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGCGA CCTCCACGCG AAGTTCGGTG ACGAGGAAAT CATCGCTATC AAAAAGTTCT 
AGTTCAGACA AATCGGCAAA GTCCTCGTCG AATTCCTCGA AATCTCCGAC AGCCGCCAGT 
GGAAACAAAC AGAAAATGCA GTACACAAAA ACCAGGGAGC GCATGGTCGA TGATGTTCCA 
CTTCCGCCCA CCCACAAGCT GACCATGTCG GAGGTATACG ACGATCCGAA GACCGGAAAA 
CCCAACTTCG ACGCCCTGCG CCAGCACTTT CTGCTGGAGG GGCGGATCGA GGAGGCGGTG 
GCCCTGAGGA TCATCACCGA AGGAGCCGCC CTGCTGCGCG AGGAGAAGAA CATGATCGAC 
GTGGAGGCAC CCATTACCGT GTGCGGTGAC ATCCACGGGC AGTTCTTCGA TCTCGTAAAG 
CTCTTCGAAG TGGGCGGCCC GCCGGCCACC ACTAGGTACC TTTTTCTAGG CGACTACGTG 
GACAGGGGAT ACTTCAGTAT TGAGTGCGTT CTCTACCTGT GGTCCCTGAA AATAACCTAC 
CCCACCACGC TTTCCCTGCT GCGAGGAAAT CACGAGTGCA GGCATCTCAC AGAGTACTTT 
ACCTTCAAGC AGGAATGCAT CATCAAGTAT TCGGAAAGTA TTTACGACGC CTGCATGGAA 
GCGTTCGACT GCCTGCCCCT CGCCGCCCTC CTCAACCAGC AGTTCCTCTG TATTCACGGC 
GGTCTGTCGC CCGAGATTTT CACACTCGAC GACATCAAGA CGCTGAATCG CTTCAGGGAA 
CCACCGGCCT ACGGGCCCAT GTGCGACCTG CTCTGGTCCG ATCCTCTGGA GGACTTTGGC 
AACGAGAAGA CCAACGAGTT CTTCTCGCAC AACTCGGTGC GCGGGTGCTC CTACTTCTTC 
AGCTACTCGG CCTGCTGTGA GTTTCTGCAG AAGAACAACC TGCTGTCGAT AGTCAGGGCG 
CACGAGGCGC AGGATGCGGG GTATCGCATG TATCGGAAGA ACCAGGTTAC GGGCTTCCCC 
TCGCTGATCA CCATATTCTC CGCCCCCAAT TATCTGGATG TTTACAATAA CAAGGCAGCT 
GTGCTCAAGT ACGAGAACAA CGTGATGAAT ATCCGCCAGT TCAACTGCTC GCCACATCCC 
TACTGGCTAC CCAACTTCAT GGACGTCTTC ACATGGTCGC TGCCCTTCGT GGGCGAAAAG 
GTAACCGAGA TGCTAGTCAA CATCCTGAAC ATTTGCTCAG ACGATGAACT GGTGGCCGGA 
CCCGATGACG AGCTCGAGGA AGCTTCCATG TCGGCGCTGC GCAAAGAGAT AATTCGGAAT 
AAGATTCGGG CCATTGGCAA GATGTCGCGC GTCTTCTCCA TCCTCAGGGA GGAATCCGAG 
AGCGTGCTCC AGCTGAAGGG TCTGACGCCC ACGGGCGCCC TGCCAGTGGG CGCTTTGTCC 
GGCGGAAGGG ATTCCCTCAA GGAGGCTCTG CAAGGACTGA CCGCATCCTC GCACATCCAC 
TCCTTTGCGG AGGCCAAGGG CCTGGATGCG GTCAACGAAC GGATGCCGCC CCGTCGTCCT 
CTTTTGATGA GTGCCAGCAG CAGCAGCATC ACCACGGTCA CAAGGAGCAG CAGCAGCAGC 
AGCAACAACA ACAACAATAA CAGCAACACC AGCAGCACCA CGACGACAAA GGACATCAGC 
AACACCAGCA GTAATGATAC CGCAACCGTA ACCAAAACGA GCAGGACGAC TGTGAAATCG 
GCCACCACCA GTAATGTGCG GGCAGGATTC ACCGCCAAGA AGTTCCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247377.1
CDS	310..2100
Translation	MSATSTRSSVTRKSSLSKSSSSDKSAKSSSNSSKSPTAASGNKQKMQYTKTRERMVDDVPLPPTHKLTMSEVYDDPKTGKPNFDALRQHFLLEGRIEEAVALRIITEGAALLREEKNMIDVEAPITVCGDIHGQFFDLVKLFEVGGPPATTRYLFLGDYVDRGYFSIECVLYLWSLKITYPTTLSLLRGNHECRHLTEYFTFKQECIIKYSESIYDACMEAFDCLPLAALLNQQFLCIHGGLSPEIFTLDDIKTLNRFREPPAYGPMCDLLWSDPLEDFGNEKTNEFFSHNSVRGCSYFFSYSACCEFLQKNNLLSIVRAHEAQDAGYRMYRKNQVTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNILNICSDDELVAGPDDELEEASMSALRKEIIRNKIRAIGKMSRVFSILREESESVLQLKGLTPTGALPVGALSGGRDSLKEALQGLTASSHIHSFAEAKGLDAVNERMPPRRPLLMSASSSSITTVTRSSSSSSNNNNNNSNTSSTTTTKDISNTSSNDTATVTKTSRTTVKSATTSNVRAGFTAKKFP

Target ORF information:

RefSeq Version	NM_001260448.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster calcineurin A1, transcript variant B (CanA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260448.2

ORF Insert Sequence:

ATGTCGGCGA CCTCCACGCG AAGTTCGGTG ACGAGGAAAT CATCGCTATC AAAAAGTTCT 
AGTTCAGACA AATCGGCAAA GTCCTCGTCG AATTCCTCGA AATCTCCGAC AGCCGCCAGT 
GGAAACAAAC AGAAAATGCA GTACACAAAA ACCAGGGAGC GCATGGTCGA TGATGTTCCA 
CTTCCGCCCA CCCACAAGCT GACCATGTCG GAGGTATACG ACGATCCGAA GACCGGAAAA 
CCCAACTTCG ACGCCCTGCG CCAGCACTTT CTGCTGGAGG GGCGGATCGA GGAGGCGGTG 
GCCCTGAGGA TCATCACCGA AGGAGCCGCC CTGCTGCGCG AGGAGAAGAA CATGATCGAC 
GTGGAGGCAC CCATTACCGT GTGCGGTGAC ATCCACGGGC AGTTCTTCGA TCTCGTAAAG 
CTCTTCGAAG TGGGCGGCCC GCCGGCCACC ACTAGGTACC TTTTTCTAGG CGACTACGTG 
GACAGGGGAT ACTTCAGTAT TGAGTGCGTT CTCTACCTGT GGTCCCTGAA AATAACCTAC 
CCCACCACGC TTTCCCTGCT GCGAGGAAAT CACGAGTGCA GGCATCTCAC AGAGTACTTT 
ACCTTCAAGC AGGAATGCAT CATCAAGTAT TCGGAAAGTA TTTACGACGC CTGCATGGAA 
GCGTTCGACT GCCTGCCCCT CGCCGCCCTC CTCAACCAGC AGTTCCTCTG TATTCACGGC 
GGTCTGTCGC CCGAGATTTT CACACTCGAC GACATCAAGA CGCTGAATCG CTTCAGGGAA 
CCACCGGCCT ACGGGCCCAT GTGCGACCTG CTCTGGTCCG ATCCTCTGGA GGACTTTGGC 
AACGAGAAGA CCAACGAGTT CTTCTCGCAC AACTCGGTGC GCGGGTGCTC CTACTTCTTC 
AGCTACTCGG CCTGCTGTGA GTTTCTGCAG AAGAACAACC TGCTGTCGAT AGTCAGGGCG 
CACGAGGCGC AGGATGCGGG GTATCGCATG TATCGGAAGA ACCAGGTTAC GGGCTTCCCC 
TCGCTGATCA CCATATTCTC CGCCCCCAAT TATCTGGATG TTTACAATAA CAAGGCAGCT 
GTGCTCAAGT ACGAGAACAA CGTGATGAAT ATCCGCCAGT TCAACTGCTC GCCACATCCC 
TACTGGCTAC CCAACTTCAT GGACGTCTTC ACATGGTCGC TGCCCTTCGT GGGCGAAAAG 
GTAACCGAGA TGCTAGTCAA CATCCTGAAC ATTTGCTCAG ACGATGAACT GGTGGCCGGA 
CCCGATGACG AGCTCGAGGA AGCTTCCATG TCGGCGCTGC GCAAAGAGAT AATTCGGAAT 
AAGATTCGGG CCATTGGCAA GATGTCGCGC GTCTTCTCCA TCCTCAGGGA GGAATCCGAG 
AGCGTGCTCC AGCTGAAGGG TCTGACGCCC ACGGGCGCCC TGCCAGTGGG CGCTTTGTCC 
GGCGGAAGGG ATTCCCTCAA GGAGGCTCTG CAAGGACTGA CCGCATCCTC GCACATCCAC 
TCCTTTGCGG AGGCCAAGGG CCTGGATGCG GTCAACGAAC GGATGCCGCC CCGTCGTCCT 
CTTTTGATGA GTGCCAGCAG CAGCAGCATC ACCACGGTCA CAAGGAGCAG CAGCAGCAGC 
AGCAACAACA ACAACAATAA CAGCAACACC AGCAGCACCA CGACGACAAA GGACATCAGC 
AACACCAGCA GTAATGATAC CGCAACCGTA ACCAAAACGA GCAGGACGAC TGTGAAATCG 
GCCACCACCA GTAATGTGCG GGCAGGATTC ACCGCCAAGA AGTTCCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CanA1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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