Tpi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpi
Entrez Gene ID	43582
Full Name	Triose phosphate isomerase
Synonyms	CG2171,Dmel\CG2171,ND14,TPI,TPIS,sgk,tpi,wstd
General protein information	Preferred Names Triose phosphate isomerase Names CG2171 gene product from transcript CG2171-RA CG2171-PA CG2171-PB CG2171-PC Tpi-PA Tpi-PB Tpi-PC neurodegeneration 14 sugarkill triose phosphate isomerase triosephosphate isomerase wasted away
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99D8-99D8\|3-101.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_176587.2	NP_788764.1	triose phosphate isomerase, isoform A
NM_176589.2	NP_788766.1	triose phosphate isomerase, isoform C
NM_176588.2	NP_788765.1	triose phosphate isomerase, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TPI1	NP_001183983.1
Mus musculus (house mouse)	Tpi1	NP_033441.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001630	XP_321467.4
Schizosaccharomyces pombe (fission yeast)	tpi1	NP_588024.1
Magnaporthe oryzae (rice blast fungus)	MGG_08905	XP_003713934.1
Arabidopsis thaliana (thale cress)	TPI	NP_191104.1
Xenopus tropicalis (tropical clawed frog)	tpi1	NP_001107706.1
Bos taurus (cattle)	TPI1	NP_001013607.1
Drosophila melanogaster (fruit fly)	Tpi	NP_788764.1
Gallus gallus (chicken)	TPI1	NP_990782.1
Kluyveromyces lactis	KLLA0F18832g	XP_455924.2
Neurospora crassa	NCU07550	XP_959063.1
Oryza sativa (rice)	Os01g0147900	NP_001042016.1
	TPI1	XP_001101998.2
Danio rerio (zebrafish)	tpi1a	NP_705953.1
Caenorhabditis elegans (roundworm)	tpi-1	NP_496563.1
Homo sapiens (human)	TPI1	NP_001152759.1
Pan troglodytes (chimpanzee)	TPI1	NP_001065250.1
Rattus norvegicus (Norway rat)	LOC100911515	XP_003750702.2
Saccharomyces cerevisiae (baker's yeast)	TPI1	NP_010335.1
Eremothecium gossypii	AGOS_AGL201C	NP_986466.2
Oryza sativa (rice)	Os01g0841600	NP_001044768.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our findings are the first to identify, to our knowledge, a functional synaptic defect in TPI deficiency derived from molecular changes in the TPI dimer interface.
Title: Structural and Genetic Studies Demonstrate Neurologic Dysfunction in Triosephosphate Isomerase Deficiency Is Associated with Impaired Synaptic Vesicle Dynamics.

TPI sugarkill can be genetically complemented by TPI encoding a catalytically inactive enzyme.
Title: Evidence of a triosephosphate isomerase non-catalytic function crucial to behavior and longevity.

Mutation of wasted away, a recessive, hypomorphic mutation of triosephosphate isomerase (Tpi), highlights the essential protective role of triosephosphate isomerase.
Title: wasted away, a Drosophila mutation in triosephosphate isomerase, causes paralysis, neurodegeneration, and early death.

The following Tpi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06613	NM_176587.2 Latest version!	Drosophila melanogaster triose phosphate isomerase, transcript variant A (Tpi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa06614	NM_176589.2 Latest version!	Drosophila melanogaster triose phosphate isomerase, transcript variant C (Tpi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06614	NM_176588.2 Latest version!	Drosophila melanogaster triose phosphate isomerase, transcript variant B (Tpi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpi ( NM_176587.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788764.1 Drosophila melanogaster triose phosphate isomerase, transcript variant A (Tpi), mRNA.

Price & Availability↑

CloneID	OFa06613
Clone ID Related Accession (Same CDS sequence)	NM_176587.2
Accession Version	NM_176587.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	triose phosphate isomerase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176587.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCTG GAGTACTCAA TGATCTGAAC AGGAACGGTA TCCAGTGCAT TGCATCAGTA 
ACGCTTCTCC AGCGATTCCC GTATCATCGG AACCACCACA CCATCCCCAC CATGGACCAG 
AACCGACCCA CTGTGGGTGG TGTTACGGCG GAGGTACTAA AAGCCGTCGT TTCGGAAGCT 
CTGCTGGGCA AATTCACAGC TGTTTTCCCA AAGCTTTTCA AGTGCCAGTG GAAGACTTAC 
GAAGGTCAAA AGAAGTTCTA CGCCAATTTC AGCACCGATT GCACCGACAG CAACAGCAAC 
AACATGAGCC GAAAGTTCTG CGTGGGAGGC AACTGGAAGA TGAACGGCGA CCAGAAGTCC 
ATCGCCGAGA TCGCCAAGAC CCTGAGCTCG GCCGCCCTCG ACCCCAACAC GGAGGTGGTC 
ATCGGCTGCC CGGCCATCTA CCTGATGTAC GCCCGCAACC TGCTGCCCTG CGAGCTGGGT 
CTGGCCGGCC AGAATGCCTA CAAGGTGGCC AAGGGCGCAT TCACCGGCGA GATCTCCCCT 
GCGATGCTGA AGGACATCGG CGCCGACTGG GTGATCCTGG GACACTCGGA GCGTCGCGCC 
ATCTTCGGCG AATCGGACGC CCTGATCGCC GAGAAGGCCG AGCACGCCCT GGCCGAGGGC 
CTCAAGGTCA TCGCCTGCAT TGGTGAGACC CTGGAGGAGC GCGAGGCCGG CAAGACCAAC 
GAGGTGGTGG CCCGCCAAAT GTGCGCCTAC GCCCAGAAGA TCAAGGACTG GAAGAACGTG 
GTGGTGGCCT ACGAGCCCGT CTGGGCCATT GGCACCGGCC AGACCGCCAC ACCCGATCAG 
GCTCAAGAGG TCCACGCCTT CCTGCGCCAG TGGCTGAGCG ACAACATCTC CAAGGAGGTG 
TCCGCCAGCC TGCGCATCCA GTACGGTGGA TCCGTGACCG CCGCCAACGC CAAGGAGCTG 
GCCAAGAAGC CCGACATAGA TGGCTTCCTG GTCGGAGGCG CCTCCCTGAA GCCCGAGTTC 
GTGGACATCA TCAACGCCCG GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788764.1
CDS	67..1113
Translation	MPPGVLNDLNRNGIQCIASVTLLQRFPYHRNHHTIPTMDQNRPTVGGVTAEVLKAVVSEALLGKFTAVFPKLFKCQWKTYEGQKKFYANFSTDCTDSNSNNMSRKFCVGGNWKMNGDQKSIAEIAKTLSSAALDPNTEVVIGCPAIYLMYARNLLPCELGLAGQNAYKVAKGAFTGEISPAMLKDIGADWVILGHSERRAIFGESDALIAEKAEHALAEGLKVIACIGETLEEREAGKTNEVVARQMCAYAQKIKDWKNVVVAYEPVWAIGTGQTATPDQAQEVHAFLRQWLSDNISKEVSASLRIQYGGSVTAANAKELAKKPDIDGFLVGGASLKPEFVDIINARQ

Target ORF information:

RefSeq Version	NM_176587.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster triose phosphate isomerase, transcript variant A (Tpi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176587.2

ORF Insert Sequence:

ATGCCGCCTG GAGTACTCAA TGATCTGAAC AGGAACGGTA TCCAGTGCAT TGCATCAGTA 
ACGCTTCTCC AGCGATTCCC GTATCATCGG AACCACCACA CCATCCCCAC CATGGACCAG 
AACCGACCCA CTGTGGGTGG TGTTACGGCG GAGGTACTAA AAGCCGTCGT TTCGGAAGCT 
CTGCTGGGCA AATTCACAGC TGTTTTCCCA AAGCTTTTCA AGTGCCAGTG GAAGACTTAC 
GAAGGTCAAA AGAAGTTCTA CGCCAATTTC AGCACCGATT GCACCGACAG CAACAGCAAC 
AACATGAGCC GAAAGTTCTG CGTGGGAGGC AACTGGAAGA TGAACGGCGA CCAGAAGTCC 
ATCGCCGAGA TCGCCAAGAC CCTGAGCTCG GCCGCCCTCG ACCCCAACAC GGAGGTGGTC 
ATCGGCTGCC CGGCCATCTA CCTGATGTAC GCCCGCAACC TGCTGCCCTG CGAGCTGGGT 
CTGGCCGGCC AGAATGCCTA CAAGGTGGCC AAGGGCGCAT TCACCGGCGA GATCTCCCCT 
GCGATGCTGA AGGACATCGG CGCCGACTGG GTGATCCTGG GACACTCGGA GCGTCGCGCC 
ATCTTCGGCG AATCGGACGC CCTGATCGCC GAGAAGGCCG AGCACGCCCT GGCCGAGGGC 
CTCAAGGTCA TCGCCTGCAT TGGTGAGACC CTGGAGGAGC GCGAGGCCGG CAAGACCAAC 
GAGGTGGTGG CCCGCCAAAT GTGCGCCTAC GCCCAGAAGA TCAAGGACTG GAAGAACGTG 
GTGGTGGCCT ACGAGCCCGT CTGGGCCATT GGCACCGGCC AGACCGCCAC ACCCGATCAG 
GCTCAAGAGG TCCACGCCTT CCTGCGCCAG TGGCTGAGCG ACAACATCTC CAAGGAGGTG 
TCCGCCAGCC TGCGCATCCA GTACGGTGGA TCCGTGACCG CCGCCAACGC CAAGGAGCTG 
GCCAAGAAGC CCGACATAGA TGGCTTCCTG GTCGGAGGCG CCTCCCTGAA GCCCGAGTTC 
GTGGACATCA TCAACGCCCG GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpi ( NM_176589.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788766.1 Drosophila melanogaster triose phosphate isomerase, transcript variant C (Tpi), mRNA.

Price & Availability↑

CloneID	OFa06614
Clone ID Related Accession (Same CDS sequence)	NM_176589.2 , NM_176588.2
Accession Version	NM_176589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	triose phosphate isomerase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176589.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCGAA AGTTCTGCGT GGGAGGCAAC TGGAAGATGA ACGGCGACCA GAAGTCCATC 
GCCGAGATCG CCAAGACCCT GAGCTCGGCC GCCCTCGACC CCAACACGGA GGTGGTCATC 
GGCTGCCCGG CCATCTACCT GATGTACGCC CGCAACCTGC TGCCCTGCGA GCTGGGTCTG 
GCCGGCCAGA ATGCCTACAA GGTGGCCAAG GGCGCATTCA CCGGCGAGAT CTCCCCTGCG 
ATGCTGAAGG ACATCGGCGC CGACTGGGTG ATCCTGGGAC ACTCGGAGCG TCGCGCCATC 
TTCGGCGAAT CGGACGCCCT GATCGCCGAG AAGGCCGAGC ACGCCCTGGC CGAGGGCCTC 
AAGGTCATCG CCTGCATTGG TGAGACCCTG GAGGAGCGCG AGGCCGGCAA GACCAACGAG 
GTGGTGGCCC GCCAAATGTG CGCCTACGCC CAGAAGATCA AGGACTGGAA GAACGTGGTG 
GTGGCCTACG AGCCCGTCTG GGCCATTGGC ACCGGCCAGA CCGCCACACC CGATCAGGCT 
CAAGAGGTCC ACGCCTTCCT GCGCCAGTGG CTGAGCGACA ACATCTCCAA GGAGGTGTCC 
GCCAGCCTGC GCATCCAGTA CGGTGGATCC GTGACCGCCG CCAACGCCAA GGAGCTGGCC 
AAGAAGCCCG ACATAGATGG CTTCCTGGTC GGAGGCGCCT CCCTGAAGCC CGAGTTCGTG 
GACATCATCA ACGCCCGGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788766.1
CDS	264..1007
Translation	MSRKFCVGGNWKMNGDQKSIAEIAKTLSSAALDPNTEVVIGCPAIYLMYARNLLPCELGLAGQNAYKVAKGAFTGEISPAMLKDIGADWVILGHSERRAIFGESDALIAEKAEHALAEGLKVIACIGETLEEREAGKTNEVVARQMCAYAQKIKDWKNVVVAYEPVWAIGTGQTATPDQAQEVHAFLRQWLSDNISKEVSASLRIQYGGSVTAANAKELAKKPDIDGFLVGGASLKPEFVDIINARQ

Target ORF information:

RefSeq Version	NM_176589.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster triose phosphate isomerase, transcript variant C (Tpi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176589.2

ORF Insert Sequence:

ATGAGCCGAA AGTTCTGCGT GGGAGGCAAC TGGAAGATGA ACGGCGACCA GAAGTCCATC 
GCCGAGATCG CCAAGACCCT GAGCTCGGCC GCCCTCGACC CCAACACGGA GGTGGTCATC 
GGCTGCCCGG CCATCTACCT GATGTACGCC CGCAACCTGC TGCCCTGCGA GCTGGGTCTG 
GCCGGCCAGA ATGCCTACAA GGTGGCCAAG GGCGCATTCA CCGGCGAGAT CTCCCCTGCG 
ATGCTGAAGG ACATCGGCGC CGACTGGGTG ATCCTGGGAC ACTCGGAGCG TCGCGCCATC 
TTCGGCGAAT CGGACGCCCT GATCGCCGAG AAGGCCGAGC ACGCCCTGGC CGAGGGCCTC 
AAGGTCATCG CCTGCATTGG TGAGACCCTG GAGGAGCGCG AGGCCGGCAA GACCAACGAG 
GTGGTGGCCC GCCAAATGTG CGCCTACGCC CAGAAGATCA AGGACTGGAA GAACGTGGTG 
GTGGCCTACG AGCCCGTCTG GGCCATTGGC ACCGGCCAGA CCGCCACACC CGATCAGGCT 
CAAGAGGTCC ACGCCTTCCT GCGCCAGTGG CTGAGCGACA ACATCTCCAA GGAGGTGTCC 
GCCAGCCTGC GCATCCAGTA CGGTGGATCC GTGACCGCCG CCAACGCCAA GGAGCTGGCC 
AAGAAGCCCG ACATAGATGG CTTCCTGGTC GGAGGCGCCT CCCTGAAGCC CGAGTTCGTG 
GACATCATCA ACGCCCGGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpi ( NM_176588.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788765.1 Drosophila melanogaster triose phosphate isomerase, transcript variant B (Tpi), mRNA.

Price & Availability↑

CloneID	OFa06614
Clone ID Related Accession (Same CDS sequence)	NM_176589.2 , NM_176588.2
Accession Version	NM_176588.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	triose phosphate isomerase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176588.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCGAA AGTTCTGCGT GGGAGGCAAC TGGAAGATGA ACGGCGACCA GAAGTCCATC 
GCCGAGATCG CCAAGACCCT GAGCTCGGCC GCCCTCGACC CCAACACGGA GGTGGTCATC 
GGCTGCCCGG CCATCTACCT GATGTACGCC CGCAACCTGC TGCCCTGCGA GCTGGGTCTG 
GCCGGCCAGA ATGCCTACAA GGTGGCCAAG GGCGCATTCA CCGGCGAGAT CTCCCCTGCG 
ATGCTGAAGG ACATCGGCGC CGACTGGGTG ATCCTGGGAC ACTCGGAGCG TCGCGCCATC 
TTCGGCGAAT CGGACGCCCT GATCGCCGAG AAGGCCGAGC ACGCCCTGGC CGAGGGCCTC 
AAGGTCATCG CCTGCATTGG TGAGACCCTG GAGGAGCGCG AGGCCGGCAA GACCAACGAG 
GTGGTGGCCC GCCAAATGTG CGCCTACGCC CAGAAGATCA AGGACTGGAA GAACGTGGTG 
GTGGCCTACG AGCCCGTCTG GGCCATTGGC ACCGGCCAGA CCGCCACACC CGATCAGGCT 
CAAGAGGTCC ACGCCTTCCT GCGCCAGTGG CTGAGCGACA ACATCTCCAA GGAGGTGTCC 
GCCAGCCTGC GCATCCAGTA CGGTGGATCC GTGACCGCCG CCAACGCCAA GGAGCTGGCC 
AAGAAGCCCG ACATAGATGG CTTCCTGGTC GGAGGCGCCT CCCTGAAGCC CGAGTTCGTG 
GACATCATCA ACGCCCGGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788765.1
CDS	108..851
Translation	MSRKFCVGGNWKMNGDQKSIAEIAKTLSSAALDPNTEVVIGCPAIYLMYARNLLPCELGLAGQNAYKVAKGAFTGEISPAMLKDIGADWVILGHSERRAIFGESDALIAEKAEHALAEGLKVIACIGETLEEREAGKTNEVVARQMCAYAQKIKDWKNVVVAYEPVWAIGTGQTATPDQAQEVHAFLRQWLSDNISKEVSASLRIQYGGSVTAANAKELAKKPDIDGFLVGGASLKPEFVDIINARQ

Target ORF information:

RefSeq Version	NM_176588.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster triose phosphate isomerase, transcript variant B (Tpi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176588.2

ORF Insert Sequence:

ATGAGCCGAA AGTTCTGCGT GGGAGGCAAC TGGAAGATGA ACGGCGACCA GAAGTCCATC 
GCCGAGATCG CCAAGACCCT GAGCTCGGCC GCCCTCGACC CCAACACGGA GGTGGTCATC 
GGCTGCCCGG CCATCTACCT GATGTACGCC CGCAACCTGC TGCCCTGCGA GCTGGGTCTG 
GCCGGCCAGA ATGCCTACAA GGTGGCCAAG GGCGCATTCA CCGGCGAGAT CTCCCCTGCG 
ATGCTGAAGG ACATCGGCGC CGACTGGGTG ATCCTGGGAC ACTCGGAGCG TCGCGCCATC 
TTCGGCGAAT CGGACGCCCT GATCGCCGAG AAGGCCGAGC ACGCCCTGGC CGAGGGCCTC 
AAGGTCATCG CCTGCATTGG TGAGACCCTG GAGGAGCGCG AGGCCGGCAA GACCAACGAG 
GTGGTGGCCC GCCAAATGTG CGCCTACGCC CAGAAGATCA AGGACTGGAA GAACGTGGTG 
GTGGCCTACG AGCCCGTCTG GGCCATTGGC ACCGGCCAGA CCGCCACACC CGATCAGGCT 
CAAGAGGTCC ACGCCTTCCT GCGCCAGTGG CTGAGCGACA ACATCTCCAA GGAGGTGTCC 
GCCAGCCTGC GCATCCAGTA CGGTGGATCC GTGACCGCCG CCAACGCCAA GGAGCTGGCC 
AAGAAGCCCG ACATAGATGG CTTCCTGGTC GGAGGCGCCT CCCTGAAGCC CGAGTTCGTG 
GACATCATCA ACGCCCGGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tpi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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