sima cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sima
Entrez Gene ID	43580
Full Name	similar
Synonyms	7951,CG31031,CG45051,CG7951,DMU43090,Dmel\CG45051,Dmel_CG31031,Dmel_CG7951,HIF alpha,HIF-1,HIF-1alpha,HIF-alpha/sima,HIF1alpha,Hif-1A,Hif1alpha,SIMA,Sima,bHLHe56,dHIF-1alpha,dHIF-alpha,l(3)j11B7
General protein information	Preferred Names similar Names CG45051 gene product from transcript CG45051-RA CG45051-PA CG45051-PB CG45051-PC CG45051-PD HIF alpha HIF-alpha hypoxia inducible factor hypoxia inducible factor ortholog hypoxia-inducible factor-1 sima-PA sima-PB sima-PC sima-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99D4-99D7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300670.1	NP_001287599.1	similar, isoform D
NM_001276171.1	NP_001263100.1	similar, isoform C
NM_079845.4	NP_524584.2	similar, isoform A
NM_001144657.3	NP_001138129.1	similar, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Musashi is a novel regulator of HIF that inhibits responses to hypoxia specifically when oxygen is available.
Title: Musashi mediates translational repression of the Drosophila hypoxia inducible factor.

most HIF1A targets require either TIP60, the CDK8-Mediator complex, or both as coactivators for full expression in hypoxia.
Title: The TIP60 Complex Is a Conserved Coactivator of HIF1A.

miR-190 is a novel regulator of the hypoxia response that represses the oxygen sensor Fatiga, leading to HIFalpha stabilization and enhancement of hypoxic responses.
Title: miR-190 Enhances HIF-Dependent Responses to Hypoxia in Drosophila by Inhibiting the Prolyl-4-hydroxylase Fatiga.

These results indicate that HIF-1alpha is required to restrain the NF-kappaB response, and thus prevents excessive and damaging pro-inflammatory responses.
Title: HIF-1? restricts NF-?B-dependent gene expression to control innate immunity signals.

crystal cells express elevated levels of Sima protein orthologous to Hif-alpha; in these cells, Sima activates Notch receptor signaling by a ligand-independent mechanism that promotes hemocyte survival during hematopoietic development and hypoxic stress
Title: Interaction between Notch and Hif-alpha in development and survival of Drosophila blood cells.

The following sima gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sima cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08962	NM_001144657.3 Latest version!	Drosophila melanogaster similar (sima), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa06610	NM_079845.4 Latest version!	Drosophila melanogaster similar (sima), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa08963	NM_001276171.1 Latest version!	Drosophila melanogaster similar (sima), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa22178	NM_001300670.1 Latest version!	Drosophila melanogaster similar (sima), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sima ( NM_001144657.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138129.1 Drosophila melanogaster similar (sima), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08962
Clone ID Related Accession (Same CDS sequence)	NM_001144657.3
Accession Version	NM_001144657.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	similar, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001144657.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTCAT TAATTGACAC AATCGAAGCG GCGGCCGAGA AACAGAAGCA GTCCCAAGCG 
GTGGTCACCA ACACCTCCGC CTCCTCCTCC TCCTGTTCCT CTTCGTTCTC GTCCTCCCCA 
CCATCTTCGT CCGTTGGCTC CCCATCTCCT GGCGCCCCAA AAACCAATCT CACGGCCAGC 
GGCAAACCAA AGGAGAAAAG AAGGAACAAT GAAAAACGCA AGGAAAAGTC TCGCGATGCG 
GCTAGATGTC GTCGCTCCAA GGAGACAGAG ATATTCATGG AACTCTCGGC TGCTTTGCCA 
TTGAAAACCG ACGATGTCAA CCAACTGGAC AAGGCCTCCG TGATGAGGAT CACCATCGCT 
TTCCTCAAGA TTCGCGAAAT GCTGCAGTTC GTGCCCAGCT TGCGCGACTG CAATGATGAC 
ATCAAGCAGG ACATTGAAAC TGCAGAGGAT CAGCAGGAAG TCAAGCCCAA GCTGGAAGTG 
GGCACTGAGG ATTGGCTCAA CGGAGCTGAG GCCAGAGAGC TCCTCAAACA GACCATGGAC 
GGATTCCTGT TGGTCTTGTC CCACGAGGGA GACATCACCT ACGTGTCCGA AAACGTAGTC 
GAGTATCTGG GCATCACCAA GATTGACACA CTTGGCCAAC AGATCTGGGA GTACTCGCAC 
CAGTGCGATC ACGCCGAGAT CAAGGAGGCT CTCAGCCTAA AGCGTGAACT TGCCCAGAAG 
GTCAAGGATG AGCCGCAGCA GAACTCTGGA GTGAGCACCC ATCACCGGGA TCTGTTTGTG 
CGCTTGAAGT GCACCTTGAC CAGTCGTGGA CGAAGCATCA ACATCAAGAG CGCTTCGTAT 
AAGGTCATTC ACATCACGGG ACACTTGGTG GTCAATGCAA AGGGCGAACG TCTGCTGATG 
GCCATTGGCC GACCAATTCC GCATCCTTCG AACATTGAGA TTCCGTTGGG AACTAGCACC 
TTCCTTACCA AACACTCCCT GGATATGCGT TTCACCTATG TGGATGACAA GATGCATGAC 
TTACTGGGAT ATTCGCCCAA GGATCTACTT GACACCTCTT TGTTTTCGTG CCAGCATGGA 
GCAGATTCCG AACGCCTGAT GGCCACCTTC AAGAGCGTGC TGAGCAAGGG ACAGGGCGAG 
ACCTCCCGCT ACAGATTTCT GGGAAAATAC GGTGGCTACT GCTGGATTCT TAGCCAGGCC 
ACGATCGTCT ACGATAAGTT AAAGCCGCAG AGCGTCGTTT GCGTTAACTA TGTCATAAGG 
ATGACGATTC CGAAGCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138129.1
CDS	615..1895
Translation	MVSLIDTIEAAAEKQKQSQAVVTNTSASSSSCSSSFSSSPPSSSVGSPSPGAPKTNLTASGKPKEKRRNNEKRKEKSRDAARCRRSKETEIFMELSAALPLKTDDVNQLDKASVMRITIAFLKIREMLQFVPSLRDCNDDIKQDIETAEDQQEVKPKLEVGTEDWLNGAEARELLKQTMDGFLLVLSHEGDITYVSENVVEYLGITKIDTLGQQIWEYSHQCDHAEIKEALSLKRELAQKVKDEPQQNSGVSTHHRDLFVRLKCTLTSRGRSINIKSASYKVIHITGHLVVNAKGERLLMAIGRPIPHPSNIEIPLGTSTFLTKHSLDMRFTYVDDKMHDLLGYSPKDLLDTSLFSCQHGADSERLMATFKSVLSKGQGETSRYRFLGKYGGYCWILSQATIVYDKLKPQSVVCVNYVIRMTIPKQ

Target ORF information:

RefSeq Version	NM_001144657.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster similar (sima), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144657.3

ORF Insert Sequence:

ATGGTTTCAT TAATTGACAC AATCGAAGCG GCGGCCGAGA AACAGAAGCA GTCCCAAGCG 
GTGGTCACCA ACACCTCCGC CTCCTCCTCC TCCTGTTCCT CTTCGTTCTC GTCCTCCCCA 
CCATCTTCGT CCGTTGGCTC CCCATCTCCT GGCGCCCCAA AAACCAATCT CACGGCCAGC 
GGCAAACCAA AGGAGAAAAG AAGGAACAAT GAAAAACGCA AGGAAAAGTC TCGCGATGCG 
GCTAGATGTC GTCGCTCCAA GGAGACAGAG ATATTCATGG AACTCTCGGC TGCTTTGCCA 
TTGAAAACCG ACGATGTCAA CCAACTGGAC AAGGCCTCCG TGATGAGGAT CACCATCGCT 
TTCCTCAAGA TTCGCGAAAT GCTGCAGTTC GTGCCCAGCT TGCGCGACTG CAATGATGAC 
ATCAAGCAGG ACATTGAAAC TGCAGAGGAT CAGCAGGAAG TCAAGCCCAA GCTGGAAGTG 
GGCACTGAGG ATTGGCTCAA CGGAGCTGAG GCCAGAGAGC TCCTCAAACA GACCATGGAC 
GGATTCCTGT TGGTCTTGTC CCACGAGGGA GACATCACCT ACGTGTCCGA AAACGTAGTC 
GAGTATCTGG GCATCACCAA GATTGACACA CTTGGCCAAC AGATCTGGGA GTACTCGCAC 
CAGTGCGATC ACGCCGAGAT CAAGGAGGCT CTCAGCCTAA AGCGTGAACT TGCCCAGAAG 
GTCAAGGATG AGCCGCAGCA GAACTCTGGA GTGAGCACCC ATCACCGGGA TCTGTTTGTG 
CGCTTGAAGT GCACCTTGAC CAGTCGTGGA CGAAGCATCA ACATCAAGAG CGCTTCGTAT 
AAGGTCATTC ACATCACGGG ACACTTGGTG GTCAATGCAA AGGGCGAACG TCTGCTGATG 
GCCATTGGCC GACCAATTCC GCATCCTTCG AACATTGAGA TTCCGTTGGG AACTAGCACC 
TTCCTTACCA AACACTCCCT GGATATGCGT TTCACCTATG TGGATGACAA GATGCATGAC 
TTACTGGGAT ATTCGCCCAA GGATCTACTT GACACCTCTT TGTTTTCGTG CCAGCATGGA 
GCAGATTCCG AACGCCTGAT GGCCACCTTC AAGAGCGTGC TGAGCAAGGG ACAGGGCGAG 
ACCTCCCGCT ACAGATTTCT GGGAAAATAC GGTGGCTACT GCTGGATTCT TAGCCAGGCC 
ACGATCGTCT ACGATAAGTT AAAGCCGCAG AGCGTCGTTT GCGTTAACTA TGTCATAAGG 
ATGACGATTC CGAAGCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sima ( NM_079845.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524584.2 Drosophila melanogaster similar (sima), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06610
Clone ID Related Accession (Same CDS sequence)	NM_079845.4
Accession Version	NM_079845.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	similar, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079845.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTCAT TAATTGACAC AATCGAAGCG GCGGCCGAGA AACAGAAGCA GTCCCAAGCG 
GTGGTCACCA ACACCTCCGC CTCCTCCTCC TCCTGTTCCT CTTCGTTCTC GTCCTCCCCA 
CCATCTTCGT CCGTTGGCTC CCCATCTCCT GGCGCCCCAA AAACCAATCT CACGGCCAGC 
GGCAAACCAA AGGAGAAAAG AAGGAACAAT GAAAAACGCA AGGAAAAGTC TCGCGATGCG 
GCTAGATGTC GTCGCTCCAA GGAGACAGAG ATATTCATGG AACTCTCGGC TGCTTTGCCA 
TTGAAAACCG ACGATGTCAA CCAACTGGAC AAGGCCTCCG TGATGAGGAT CACCATCGCT 
TTCCTCAAGA TTCGCGAAAT GCTGCAGTTC GTGCCCAGCT TGCGCGACTG CAATGATGAC 
ATCAAGCAGG ACATTGAAAC TGCAGAGGAT CAGCAGGAAG TCAAGCCCAA GCTGGAAGTG 
GGCACTGAGG ATTGGCTCAA CGGAGCTGAG GCCAGAGAGC TCCTCAAACA GACCATGGAC 
GGATTCCTGT TGGTCTTGTC CCACGAGGGA GACATCACCT ACGTGTCCGA AAACGTAGTC 
GAGTATCTGG GCATCACCAA GATTGACACA CTTGGCCAAC AGATCTGGGA GTACTCGCAC 
CAGTGCGATC ACGCCGAGAT CAAGGAGGCT CTCAGCCTAA AGCGTGAACT TGCCCAGAAG 
GTCAAGGATG AGCCGCAGCA GAACTCTGGA GTGAGCACCC ATCACCGGGA TCTGTTTGTG 
CGCTTGAAGT GCACCTTGAC CAGTCGTGGA CGAAGCATCA ACATCAAGAG CGCTTCGTAT 
AAGGTCATTC ACATCACGGG ACACTTGGTG GTCAATGCAA AGGGCGAACG TCTGCTGATG 
GCCATTGGCC GACCAATTCC GCATCCTTCG AACATTGAGA TTCCGTTGGG AACTAGCACC 
TTCCTTACCA AACACTCCCT GGATATGCGT TTCACCTATG TGGATGACAA GATGCATGAC 
TTACTGGGAT ATTCGCCCAA GGATCTACTT GACACCTCTT TGTTTTCGTG CCAGCATGGA 
GCAGATTCCG AACGCCTGAT GGCCACCTTC AAGAGCGTGC TGAGCAAGGG ACAGGGCGAG 
ACCTCCCGCT ACAGATTTCT GGGAAAATAC GGTGGCTACT GCTGGATTCT TAGCCAGGCC 
ACGATCGTCT ACGATAAGTT AAAGCCGCAG AGCGTCGTTT GCGTTAACTA TGTCATAAGT 
AATCTTGAGA ATAAACATGA GATATACTCA CTTGCTCAAC AAACGGCGGC AAGTGAGCAA 
AAGGAACAAC ACCACCAAGC AGCTGAAACC GAGAAGGAGC CCGAAAAAGC AGCCGATCCC 
GAAATAATCG CTCAGGAAAC CAAGGAAACA GTAAACACAC CTATTCACAC CTCAGAGCTG 
CAAGCTAAGC CATTGCAACT TGAGAGCGAG AAAGCAGAGA AAACCATCGA GGAAACCAAA 
ACAATTGCAA CAATCCCACC AGTAACTGCA ACCTCAACGG CTGACCAGAT AAAGCAGCTG 
CCCGAGAGCA ATCCGTACAA GCAGATCCTC CAGGCGGAGT TGCTCATCAA GCGGGAGAAC 
CACTCGCCAG GACCGCGCAC CATCACTGCC CAGCTGCTCA GCGGCAGTAG CAGCGGACTG 
CGTCCCGAGG AGAAGCGTCC CAAGTCGGTG ACCGCTTCGG TCCTCCGCCC GAGTCCAGCT 
CCACCCCTAA CACCACCGCC CACAGCGGTG CTCTGCAAGA AGACTCCGCT CGGCGTGGAG 
CCCAATCTGC CGCCAACCAC CACCGCTACG GCAGCCATAA TCTCCTCGAG CAATCAGCAG 
CTGCAGATCG CACAGCAAAC CCAACTGCAG AATCCCCAGC AACCAGCTCA AGATATGAGT 
AAAGGATTCT GTTCGCTATT TGCCGACGAT GGACGAGGTC TGACAATGCT TAAGGAAGAA 
CCTGACGATT TGTCCCACCA CCTGGCCTCC ACCAATTGCA TTCAGCTGGA CGAGATGACT 
CCCTTCAGTG ACATGTTGGT TGGCCTCATG GGCACCTGCC TGCTGCCCGA GGATATCAAT 
TCCCTGGACT CGACCACCTG CTCCACGACG GCCAGCGGCC AGCACTACCA GAGCCCCAGC 
AGCAGCAGCA CCTCTGCACC CAGCAATACC AGTAGCAGCA ATAATAGCTA TGCCAACAGT 
CCGCTCAGCC CGCTTACACC CAATTCCACG GCAACCGCTA GCAATCCCAG TCATCAGCAG 
CAACAGCAAC ATCACAACCA GCAGCAGCAG CAGCAACAAC AGCAGCAGCA TCATCCCCAG 
CACCACGACA ACAGCAACAG CAGCAGCAAC ATCGATCCGC TGTTCAACTA TCGCGAGGAG 
TCGAATGACA CAAGCTGCTC GCAACATCTG CATTCGCCTA GCATCACATC AAAGAGTCCC 
GAGGACAGCA GCCTCCCATC GTTGTGCAGT CCCAATTCAC TAACACAGGA GGATGACTTC 
TCTTTCGAGG CCTTTGCCAT GCGTGCGCCC TACATCCCGA TCGATGATGA TATGCCCCTG 
CTAACGGAGA CGGATCTCAT GTGGTGCCCA CCAGAGGATC TGCAGACCAT GGTGCCCAAG 
GAGATCGACG CCATACAGCA GCAGTTGCAA CAGTTGCAGC AGCAACATCA CCAGCAATAC 
GCCGGCAACA CTGGCTACCA GCAGCAGCAG CAGCAGCCGC AACTCCAGCA GCAGCATTTC 
TCCAACTCCC TGTGCTCCTC GCCAGCTTCA ACGGTCTCGT CATTGTCGCC ATCGCCCGTG 
CAACAGCATC ATCAGCAGCA ACAGGCAGCG GTCTTCACCA GCGATTCCAG CGAACTGGCG 
GCATTGCTGT GCGGATCCGG AAATGGAACA CTTTCGATCC TGGCAGGTAG TGGTGTTACG 
GTTGCGGAGG AGTGCAACGA GCGGCTGCAG CAACATCAGC AGCAGCAACA GCAGACGAGT 
GGCAACGAGT TCAGGACTTT CCAGCAGCTG CAGCAGGAGT TGCAGTTGCA GGAGGAACAG 
CAGCAGCGTC AGCAGCAGCA ACAACAGCAG CAACAGCAGC AGCAGCAACA GCAGTTACTC 
AGCCTAAATA TCGAGTGCAA AAAAGAGAAA TACGATGTGC AAATGGGAGG CAGTTTATGT 
CATCCCATGG AGGATGCATT TGAGAATGAC TACAGCAAGG ATTCCGCCAA TCTGGACTGC 
TGGGACCTGA TTCAAATGCA GGTGGTGGAC ACGGAACCCG TGAGTCCCAA TGCCGCATCT 
CCCACTCCCT GCAAGGTGTC TGCCATCCAG TTGCTCCAGC AACAGCAGCA ACTGCAGCAG 
CAGCAGCAGC AGCAGCAGAA CATTATCTTG AATGCAGTGC CATTGATTAC CATACAGAAT 
AACAAGGAAT TGATGCAACA GCAGCAGCAG CAGCAGCAAC AGCAACAGCA GGAGCAGCTG 
CAGCAGCCGG CTATAAAACT GCTGAATGGA GCCAGCATTG CGCCGGTGAA CACCAAGGCC 
ACCATCCGCC TGGTGGAGAG CAAGCCGCCC ACCACGACGC AGTCTCGAAT GGCGAAGGTG 
AACCTCGTGC CGCAGCAGCA GCAGCATGGA AACAAGCGCC ACCTGAACAG CGCGACAGGA 
GCGGGCAATC CCGTGGAGTC CAAGCGTCTG AAGAGCGGAA CCCTCTGCCT GGACGTGCAG 
TCCCCTCAGC TCCTGCAGCA GCTAATTGGC AAGGATCCTG CCCAGCAGCA AACCCAAGCA 
GCCAAGCGAG CAGGCAGTGA GCGCTGGCAA TTGTCGGCGG AGAGTAAACA GCAGAAGCAG 
CAACAACAGC AGTCGAACTC GGTACTAAAG AACCTGCTGG TCAGCGGACG CGATGATGAC 
GATTCCGAAG CAATGATAAT CGATGAGGAC AACTCCCTGG TGCAGCCAAT TCCACTGGGC 
AAATATGGCT TGCCCCTGCA CTGTCATACC TCCACGTCTT CGGTGCTGCG GGACTATCAT 
AACAACCCGC TGATCAGTGG CACCAACTTC CAGCTGTCTC CAGTCTTCGG AGGCTCCGAT 
TCCAGTGGAG GTGACGGCGA GACTGGGTCA GTGGTTTCCC TAGATGACTC GGTGCCACCC 
GGCTTGACAG CCTGCGACAC GGATGCCTCC AGCGACAGTG GCATCGATGA GAACAGCCTG 
ATGGATGGGG CATCGGGGTC ACCGCGTAAG AGGTTGTCCT CAACTTCCAA TAGCACTAAT 
CAGGCTGAGT CGGCACCACC GGCTTTGGAC GTGGAGACTC CAGTGACCCA GAAGTCGGTA 
GAAGAGGAGT TCGAGGGAGG CGGAAGCGGC AGTAACGCCC CCAGCAGGAA GACTTCCATT 
TCCTTCCTGG ACAGCAGCAA TCCCCTGCTG CACACGCCGG CGATGATGGA CCTGGTCAAC 
GACGACTACA TCATGGGCGA GGGTGGCTTC GAGTTTAGCG ACAACCAGTT GGAGCAAGTC 
CTGGGTTGGC CAGAAATCGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524584.2
CDS	615..5138
Translation	MVSLIDTIEAAAEKQKQSQAVVTNTSASSSSCSSSFSSSPPSSSVGSPSPGAPKTNLTASGKPKEKRRNNEKRKEKSRDAARCRRSKETEIFMELSAALPLKTDDVNQLDKASVMRITIAFLKIREMLQFVPSLRDCNDDIKQDIETAEDQQEVKPKLEVGTEDWLNGAEARELLKQTMDGFLLVLSHEGDITYVSENVVEYLGITKIDTLGQQIWEYSHQCDHAEIKEALSLKRELAQKVKDEPQQNSGVSTHHRDLFVRLKCTLTSRGRSINIKSASYKVIHITGHLVVNAKGERLLMAIGRPIPHPSNIEIPLGTSTFLTKHSLDMRFTYVDDKMHDLLGYSPKDLLDTSLFSCQHGADSERLMATFKSVLSKGQGETSRYRFLGKYGGYCWILSQATIVYDKLKPQSVVCVNYVISNLENKHEIYSLAQQTAASEQKEQHHQAAETEKEPEKAADPEIIAQETKETVNTPIHTSELQAKPLQLESEKAEKTIEETKTIATIPPVTATSTADQIKQLPESNPYKQILQAELLIKRENHSPGPRTITAQLLSGSSSGLRPEEKRPKSVTASVLRPSPAPPLTPPPTAVLCKKTPLGVEPNLPPTTTATAAIISSSNQQLQIAQQTQLQNPQQPAQDMSKGFCSLFADDGRGLTMLKEEPDDLSHHLASTNCIQLDEMTPFSDMLVGLMGTCLLPEDINSLDSTTCSTTASGQHYQSPSSSSTSAPSNTSSSNNSYANSPLSPLTPNSTATASNPSHQQQQQHHNQQQQQQQQQQHHPQHHDNSNSSSNIDPLFNYREESNDTSCSQHLHSPSITSKSPEDSSLPSLCSPNSLTQEDDFSFEAFAMRAPYIPIDDDMPLLTETDLMWCPPEDLQTMVPKEIDAIQQQLQQLQQQHHQQYAGNTGYQQQQQQPQLQQQHFSNSLCSSPASTVSSLSPSPVQQHHQQQQAAVFTSDSSELAALLCGSGNGTLSILAGSGVTVAEECNERLQQHQQQQQQTSGNEFRTFQQLQQELQLQEEQQQRQQQQQQQQQQQQQQQLLSLNIECKKEKYDVQMGGSLCHPMEDAFENDYSKDSANLDCWDLIQMQVVDTEPVSPNAASPTPCKVSAIQLLQQQQQLQQQQQQQQNIILNAVPLITIQNNKELMQQQQQQQQQQQQEQLQQPAIKLLNGASIAPVNTKATIRLVESKPPTTTQSRMAKVNLVPQQQQHGNKRHLNSATGAGNPVESKRLKSGTLCLDVQSPQLLQQLIGKDPAQQQTQAAKRAGSERWQLSAESKQQKQQQQQSNSVLKNLLVSGRDDDDSEAMIIDEDNSLVQPIPLGKYGLPLHCHTSTSSVLRDYHNNPLISGTNFQLSPVFGGSDSSGGDGETGSVVSLDDSVPPGLTACDTDASSDSGIDENSLMDGASGSPRKRLSSTSNSTNQAESAPPALDVETPVTQKSVEEEFEGGGSGSNAPSRKTSISFLDSSNPLLHTPAMMDLVNDDYIMGEGGFEFSDNQLEQVLGWPEIA

Target ORF information:

RefSeq Version	NM_079845.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster similar (sima), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079845.4

ORF Insert Sequence:

ATGGTTTCAT TAATTGACAC AATCGAAGCG GCGGCCGAGA AACAGAAGCA GTCCCAAGCG 
GTGGTCACCA ACACCTCCGC CTCCTCCTCC TCCTGTTCCT CTTCGTTCTC GTCCTCCCCA 
CCATCTTCGT CCGTTGGCTC CCCATCTCCT GGCGCCCCAA AAACCAATCT CACGGCCAGC 
GGCAAACCAA AGGAGAAAAG AAGGAACAAT GAAAAACGCA AGGAAAAGTC TCGCGATGCG 
GCTAGATGTC GTCGCTCCAA GGAGACAGAG ATATTCATGG AACTCTCGGC TGCTTTGCCA 
TTGAAAACCG ACGATGTCAA CCAACTGGAC AAGGCCTCCG TGATGAGGAT CACCATCGCT 
TTCCTCAAGA TTCGCGAAAT GCTGCAGTTC GTGCCCAGCT TGCGCGACTG CAATGATGAC 
ATCAAGCAGG ACATTGAAAC TGCAGAGGAT CAGCAGGAAG TCAAGCCCAA GCTGGAAGTG 
GGCACTGAGG ATTGGCTCAA CGGAGCTGAG GCCAGAGAGC TCCTCAAACA GACCATGGAC 
GGATTCCTGT TGGTCTTGTC CCACGAGGGA GACATCACCT ACGTGTCCGA AAACGTAGTC 
GAGTATCTGG GCATCACCAA GATTGACACA CTTGGCCAAC AGATCTGGGA GTACTCGCAC 
CAGTGCGATC ACGCCGAGAT CAAGGAGGCT CTCAGCCTAA AGCGTGAACT TGCCCAGAAG 
GTCAAGGATG AGCCGCAGCA GAACTCTGGA GTGAGCACCC ATCACCGGGA TCTGTTTGTG 
CGCTTGAAGT GCACCTTGAC CAGTCGTGGA CGAAGCATCA ACATCAAGAG CGCTTCGTAT 
AAGGTCATTC ACATCACGGG ACACTTGGTG GTCAATGCAA AGGGCGAACG TCTGCTGATG 
GCCATTGGCC GACCAATTCC GCATCCTTCG AACATTGAGA TTCCGTTGGG AACTAGCACC 
TTCCTTACCA AACACTCCCT GGATATGCGT TTCACCTATG TGGATGACAA GATGCATGAC 
TTACTGGGAT ATTCGCCCAA GGATCTACTT GACACCTCTT TGTTTTCGTG CCAGCATGGA 
GCAGATTCCG AACGCCTGAT GGCCACCTTC AAGAGCGTGC TGAGCAAGGG ACAGGGCGAG 
ACCTCCCGCT ACAGATTTCT GGGAAAATAC GGTGGCTACT GCTGGATTCT TAGCCAGGCC 
ACGATCGTCT ACGATAAGTT AAAGCCGCAG AGCGTCGTTT GCGTTAACTA TGTCATAAGT 
AATCTTGAGA ATAAACATGA GATATACTCA CTTGCTCAAC AAACGGCGGC AAGTGAGCAA 
AAGGAACAAC ACCACCAAGC AGCTGAAACC GAGAAGGAGC CCGAAAAAGC AGCCGATCCC 
GAAATAATCG CTCAGGAAAC CAAGGAAACA GTAAACACAC CTATTCACAC CTCAGAGCTG 
CAAGCTAAGC CATTGCAACT TGAGAGCGAG AAAGCAGAGA AAACCATCGA GGAAACCAAA 
ACAATTGCAA CAATCCCACC AGTAACTGCA ACCTCAACGG CTGACCAGAT AAAGCAGCTG 
CCCGAGAGCA ATCCGTACAA GCAGATCCTC CAGGCGGAGT TGCTCATCAA GCGGGAGAAC 
CACTCGCCAG GACCGCGCAC CATCACTGCC CAGCTGCTCA GCGGCAGTAG CAGCGGACTG 
CGTCCCGAGG AGAAGCGTCC CAAGTCGGTG ACCGCTTCGG TCCTCCGCCC GAGTCCAGCT 
CCACCCCTAA CACCACCGCC CACAGCGGTG CTCTGCAAGA AGACTCCGCT CGGCGTGGAG 
CCCAATCTGC CGCCAACCAC CACCGCTACG GCAGCCATAA TCTCCTCGAG CAATCAGCAG 
CTGCAGATCG CACAGCAAAC CCAACTGCAG AATCCCCAGC AACCAGCTCA AGATATGAGT 
AAAGGATTCT GTTCGCTATT TGCCGACGAT GGACGAGGTC TGACAATGCT TAAGGAAGAA 
CCTGACGATT TGTCCCACCA CCTGGCCTCC ACCAATTGCA TTCAGCTGGA CGAGATGACT 
CCCTTCAGTG ACATGTTGGT TGGCCTCATG GGCACCTGCC TGCTGCCCGA GGATATCAAT 
TCCCTGGACT CGACCACCTG CTCCACGACG GCCAGCGGCC AGCACTACCA GAGCCCCAGC 
AGCAGCAGCA CCTCTGCACC CAGCAATACC AGTAGCAGCA ATAATAGCTA TGCCAACAGT 
CCGCTCAGCC CGCTTACACC CAATTCCACG GCAACCGCTA GCAATCCCAG TCATCAGCAG 
CAACAGCAAC ATCACAACCA GCAGCAGCAG CAGCAACAAC AGCAGCAGCA TCATCCCCAG 
CACCACGACA ACAGCAACAG CAGCAGCAAC ATCGATCCGC TGTTCAACTA TCGCGAGGAG 
TCGAATGACA CAAGCTGCTC GCAACATCTG CATTCGCCTA GCATCACATC AAAGAGTCCC 
GAGGACAGCA GCCTCCCATC GTTGTGCAGT CCCAATTCAC TAACACAGGA GGATGACTTC 
TCTTTCGAGG CCTTTGCCAT GCGTGCGCCC TACATCCCGA TCGATGATGA TATGCCCCTG 
CTAACGGAGA CGGATCTCAT GTGGTGCCCA CCAGAGGATC TGCAGACCAT GGTGCCCAAG 
GAGATCGACG CCATACAGCA GCAGTTGCAA CAGTTGCAGC AGCAACATCA CCAGCAATAC 
GCCGGCAACA CTGGCTACCA GCAGCAGCAG CAGCAGCCGC AACTCCAGCA GCAGCATTTC 
TCCAACTCCC TGTGCTCCTC GCCAGCTTCA ACGGTCTCGT CATTGTCGCC ATCGCCCGTG 
CAACAGCATC ATCAGCAGCA ACAGGCAGCG GTCTTCACCA GCGATTCCAG CGAACTGGCG 
GCATTGCTGT GCGGATCCGG AAATGGAACA CTTTCGATCC TGGCAGGTAG TGGTGTTACG 
GTTGCGGAGG AGTGCAACGA GCGGCTGCAG CAACATCAGC AGCAGCAACA GCAGACGAGT 
GGCAACGAGT TCAGGACTTT CCAGCAGCTG CAGCAGGAGT TGCAGTTGCA GGAGGAACAG 
CAGCAGCGTC AGCAGCAGCA ACAACAGCAG CAACAGCAGC AGCAGCAACA GCAGTTACTC 
AGCCTAAATA TCGAGTGCAA AAAAGAGAAA TACGATGTGC AAATGGGAGG CAGTTTATGT 
CATCCCATGG AGGATGCATT TGAGAATGAC TACAGCAAGG ATTCCGCCAA TCTGGACTGC 
TGGGACCTGA TTCAAATGCA GGTGGTGGAC ACGGAACCCG TGAGTCCCAA TGCCGCATCT 
CCCACTCCCT GCAAGGTGTC TGCCATCCAG TTGCTCCAGC AACAGCAGCA ACTGCAGCAG 
CAGCAGCAGC AGCAGCAGAA CATTATCTTG AATGCAGTGC CATTGATTAC CATACAGAAT 
AACAAGGAAT TGATGCAACA GCAGCAGCAG CAGCAGCAAC AGCAACAGCA GGAGCAGCTG 
CAGCAGCCGG CTATAAAACT GCTGAATGGA GCCAGCATTG CGCCGGTGAA CACCAAGGCC 
ACCATCCGCC TGGTGGAGAG CAAGCCGCCC ACCACGACGC AGTCTCGAAT GGCGAAGGTG 
AACCTCGTGC CGCAGCAGCA GCAGCATGGA AACAAGCGCC ACCTGAACAG CGCGACAGGA 
GCGGGCAATC CCGTGGAGTC CAAGCGTCTG AAGAGCGGAA CCCTCTGCCT GGACGTGCAG 
TCCCCTCAGC TCCTGCAGCA GCTAATTGGC AAGGATCCTG CCCAGCAGCA AACCCAAGCA 
GCCAAGCGAG CAGGCAGTGA GCGCTGGCAA TTGTCGGCGG AGAGTAAACA GCAGAAGCAG 
CAACAACAGC AGTCGAACTC GGTACTAAAG AACCTGCTGG TCAGCGGACG CGATGATGAC 
GATTCCGAAG CAATGATAAT CGATGAGGAC AACTCCCTGG TGCAGCCAAT TCCACTGGGC 
AAATATGGCT TGCCCCTGCA CTGTCATACC TCCACGTCTT CGGTGCTGCG GGACTATCAT 
AACAACCCGC TGATCAGTGG CACCAACTTC CAGCTGTCTC CAGTCTTCGG AGGCTCCGAT 
TCCAGTGGAG GTGACGGCGA GACTGGGTCA GTGGTTTCCC TAGATGACTC GGTGCCACCC 
GGCTTGACAG CCTGCGACAC GGATGCCTCC AGCGACAGTG GCATCGATGA GAACAGCCTG 
ATGGATGGGG CATCGGGGTC ACCGCGTAAG AGGTTGTCCT CAACTTCCAA TAGCACTAAT 
CAGGCTGAGT CGGCACCACC GGCTTTGGAC GTGGAGACTC CAGTGACCCA GAAGTCGGTA 
GAAGAGGAGT TCGAGGGAGG CGGAAGCGGC AGTAACGCCC CCAGCAGGAA GACTTCCATT 
TCCTTCCTGG ACAGCAGCAA TCCCCTGCTG CACACGCCGG CGATGATGGA CCTGGTCAAC 
GACGACTACA TCATGGGCGA GGGTGGCTTC GAGTTTAGCG ACAACCAGTT GGAGCAAGTC 
CTGGGTTGGC CAGAAATCGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sima ( NM_001276171.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263100.1 Drosophila melanogaster similar (sima), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08963
Clone ID Related Accession (Same CDS sequence)	NM_001276171.1
Accession Version	NM_001276171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	similar, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTAAAG GATTCTGTTC GCTATTTGCC GACGATGGAC GAGGTCTGAC AATGCTTAAG 
GAAGAACCTG ACGATTTGTC CCACCACCTG GCCTCCACCA ATTGCATTCA GCTGGACGAG 
ATGACTCCCT TCAGTGACAT GTTGGTTGGC CTCATGGGCA CCTGCCTGCT GCCCGAGGAT 
ATCAATTCCC TGGACTCGAC CACCTGCTCC ACGACGGCCA GCGGCCAGCA CTACCAGAGC 
CCCAGCAGCA GCAGCACCTC TGCACCCAGC AATACCAGTA GCAGCAATAA TAGCTATGCC 
AACAGTCCGC TCAGCCCGCT TACACCCAAT TCCACGGCAA CCGCTAGCAA TCCCAGTCAT 
CAGCAGCAAC AGCAACATCA CAACCAGCAG CAGCAGCAGC AACAACAGCA GCAGCATCAT 
CCCCAGCACC ACGACAACAG CAACAGCAGC AGCAACATCG ATCCGCTGTT CAACTATCGC 
GAGGAGTCGA ATGACACAAG CTGCTCGCAA CATCTGCATT CGCCTAGCAT CACATCAAAG 
AGTCCCGAGG ACAGCAGCCT CCCATCGTTG TGCAGTCCCA ATTCACTAAC ACAGGAGGAT 
GACTTCTCTT TCGAGGCCTT TGCCATGCGT GCGCCCTACA TCCCGATCGA TGATGATATG 
CCCCTGCTAA CGGAGACGGA TCTCATGTGG TGCCCACCAG AGGATCTGCA GACCATGGTG 
CCCAAGGAGA TCGACGCCAT ACAGCAGCAG TTGCAACAGT TGCAGCAGCA ACATCACCAG 
CAATACGCCG GCAACACTGG CTACCAGCAG CAGCAGCAGC AGCCGCAACT CCAGCAGCAG 
CATTTCTCCA ACTCCCTGTG CTCCTCGCCA GCTTCAACGG TCTCGTCATT GTCGCCATCG 
CCCGTGCAAC AGCATCATCA GCAGCAACAG GCAGCGGTCT TCACCAGCGA TTCCAGCGAA 
CTGGCGGCAT TGCTGTGCGG ATCCGGAAAT GGAACACTTT CGATCCTGGC AGGTAGTGGT 
GTTACGGTTG CGGAGGAGTG CAACGAGCGG CTGCAGCAAC ATCAGCAGCA GCAACAGCAG 
ACGAGTGGCA ACGAGTTCAG GACTTTCCAG CAGCTGCAGC AGGAGTTGCA GTTGCAGGAG 
GAACAGCAGC AGCGTCAGCA GCAGCAACAA CAGCAGCAAC AGCAGCAGCA GCAACAGCAG 
TTACTCAGCC TAAATATCGA GTGCAAAAAA GAGAAATACG ATGTGCAAAT GGGAGGCAGT 
TTATGTCATC CCATGGAGGA TGCATTTGAG AATGACTACA GCAAGGATTC CGCCAATCTG 
GACTGCTGGG ACCTGATTCA AATGCAGGTG GTGGACACGG AACCCGTGAG TCCCAATGCC 
GCATCTCCCA CTCCCTGCAA GGTGTCTGCC ATCCAGTTGC TCCAGCAACA GCAGCAACTG 
CAGCAGCAGC AGCAGCAGCA GCAGAACATT ATCTTGAATG CAGTGCCATT GATTACCATA 
CAGAATAACA AGGAATTGAT GCAACAGCAG CAGCAGCAGC AGCAACAGCA ACAGCAGGAG 
CAGCTGCAGC AGCCGGCTAT AAAACTGCTG AATGGAGCCA GCATTGCGCC GGTGAACACC 
AAGGCCACCA TCCGCCTGGT GGAGAGCAAG CCGCCCACCA CGACGCAGTC TCGAATGGCG 
AAGGTGAACC TCGTGCCGCA GCAGCAGCAG CATGGAAACA AGCGCCACCT GAACAGCGCG 
ACAGGAGCGG GCAATCCCGT GGAGTCCAAG CGTCTGAAGA GCGGAACCCT CTGCCTGGAC 
GTGCAGTCCC CTCAGCTCCT GCAGCAGCTA ATTGGCAAGG ATCCTGCCCA GCAGCAAACC 
CAAGCAGCCA AGCGAGCAGG CAGTGAGCGC TGGCAATTGT CGGCGGAGAG TAAACAGCAG 
AAGCAGCAAC AACAGCAGTC GAACTCGGTA CTAAAGAACC TGCTGGTCAG CGGACGCGAT 
GATGACGATT CCGAAGCAAT GATAATCGAT GAGGACAACT CCCTGGTGCA GCCAATTCCA 
CTGGGCAAAT ATGGCTTGCC CCTGCACTGT CATACCTCCA CGTCTTCGGT GCTGCGGGAC 
TATCATAACA ACCCGCTGAT CAGTGGCACC AACTTCCAGC TGTCTCCAGT CTTCGGAGGC 
TCCGATTCCA GTGGAGGTGA CGGCGAGACT GGGTCAGTGG TTTCCCTAGA TGACTCGGTG 
CCACCCGGCT TGACAGCCTG CGACACGGAT GCCTCCAGCG ACAGTGGCAT CGATGAGAAC 
AGCCTGATGG ATGGGGCATC GGGGTCACCG CGTAAGAGGT TGTCCTCAAC TTCCAATAGC 
ACTAATCAGG CTGAGTCGGC ACCACCGGCT TTGGACGTGG AGACTCCAGT GACCCAGAAG 
TCGGTAGAAG AGGAGTTCGA GGGAGGCGGA AGCGGCAGTA ACGCCCCCAG CAGGAAGACT 
TCCATTTCCT TCCTGGACAG CAGCAATCCC CTGCTGCACA CGCCGGCGAT GATGGACCTG 
GTCAACGACG ACTACATCAT GGGCGAGGGT GGCTTCGAGT TTAGCGACAA CCAGTTGGAG 
CAAGTCCTGG GTTGGCCAGA AATCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263100.1
CDS	1055..3664
Translation	MSKGFCSLFADDGRGLTMLKEEPDDLSHHLASTNCIQLDEMTPFSDMLVGLMGTCLLPEDINSLDSTTCSTTASGQHYQSPSSSSTSAPSNTSSSNNSYANSPLSPLTPNSTATASNPSHQQQQQHHNQQQQQQQQQQHHPQHHDNSNSSSNIDPLFNYREESNDTSCSQHLHSPSITSKSPEDSSLPSLCSPNSLTQEDDFSFEAFAMRAPYIPIDDDMPLLTETDLMWCPPEDLQTMVPKEIDAIQQQLQQLQQQHHQQYAGNTGYQQQQQQPQLQQQHFSNSLCSSPASTVSSLSPSPVQQHHQQQQAAVFTSDSSELAALLCGSGNGTLSILAGSGVTVAEECNERLQQHQQQQQQTSGNEFRTFQQLQQELQLQEEQQQRQQQQQQQQQQQQQQQLLSLNIECKKEKYDVQMGGSLCHPMEDAFENDYSKDSANLDCWDLIQMQVVDTEPVSPNAASPTPCKVSAIQLLQQQQQLQQQQQQQQNIILNAVPLITIQNNKELMQQQQQQQQQQQQEQLQQPAIKLLNGASIAPVNTKATIRLVESKPPTTTQSRMAKVNLVPQQQQHGNKRHLNSATGAGNPVESKRLKSGTLCLDVQSPQLLQQLIGKDPAQQQTQAAKRAGSERWQLSAESKQQKQQQQQSNSVLKNLLVSGRDDDDSEAMIIDEDNSLVQPIPLGKYGLPLHCHTSTSSVLRDYHNNPLISGTNFQLSPVFGGSDSSGGDGETGSVVSLDDSVPPGLTACDTDASSDSGIDENSLMDGASGSPRKRLSSTSNSTNQAESAPPALDVETPVTQKSVEEEFEGGGSGSNAPSRKTSISFLDSSNPLLHTPAMMDLVNDDYIMGEGGFEFSDNQLEQVLGWPEIA

Target ORF information:

RefSeq Version	NM_001276171.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster similar (sima), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276171.1

ORF Insert Sequence:

ATGAGTAAAG GATTCTGTTC GCTATTTGCC GACGATGGAC GAGGTCTGAC AATGCTTAAG 
GAAGAACCTG ACGATTTGTC CCACCACCTG GCCTCCACCA ATTGCATTCA GCTGGACGAG 
ATGACTCCCT TCAGTGACAT GTTGGTTGGC CTCATGGGCA CCTGCCTGCT GCCCGAGGAT 
ATCAATTCCC TGGACTCGAC CACCTGCTCC ACGACGGCCA GCGGCCAGCA CTACCAGAGC 
CCCAGCAGCA GCAGCACCTC TGCACCCAGC AATACCAGTA GCAGCAATAA TAGCTATGCC 
AACAGTCCGC TCAGCCCGCT TACACCCAAT TCCACGGCAA CCGCTAGCAA TCCCAGTCAT 
CAGCAGCAAC AGCAACATCA CAACCAGCAG CAGCAGCAGC AACAACAGCA GCAGCATCAT 
CCCCAGCACC ACGACAACAG CAACAGCAGC AGCAACATCG ATCCGCTGTT CAACTATCGC 
GAGGAGTCGA ATGACACAAG CTGCTCGCAA CATCTGCATT CGCCTAGCAT CACATCAAAG 
AGTCCCGAGG ACAGCAGCCT CCCATCGTTG TGCAGTCCCA ATTCACTAAC ACAGGAGGAT 
GACTTCTCTT TCGAGGCCTT TGCCATGCGT GCGCCCTACA TCCCGATCGA TGATGATATG 
CCCCTGCTAA CGGAGACGGA TCTCATGTGG TGCCCACCAG AGGATCTGCA GACCATGGTG 
CCCAAGGAGA TCGACGCCAT ACAGCAGCAG TTGCAACAGT TGCAGCAGCA ACATCACCAG 
CAATACGCCG GCAACACTGG CTACCAGCAG CAGCAGCAGC AGCCGCAACT CCAGCAGCAG 
CATTTCTCCA ACTCCCTGTG CTCCTCGCCA GCTTCAACGG TCTCGTCATT GTCGCCATCG 
CCCGTGCAAC AGCATCATCA GCAGCAACAG GCAGCGGTCT TCACCAGCGA TTCCAGCGAA 
CTGGCGGCAT TGCTGTGCGG ATCCGGAAAT GGAACACTTT CGATCCTGGC AGGTAGTGGT 
GTTACGGTTG CGGAGGAGTG CAACGAGCGG CTGCAGCAAC ATCAGCAGCA GCAACAGCAG 
ACGAGTGGCA ACGAGTTCAG GACTTTCCAG CAGCTGCAGC AGGAGTTGCA GTTGCAGGAG 
GAACAGCAGC AGCGTCAGCA GCAGCAACAA CAGCAGCAAC AGCAGCAGCA GCAACAGCAG 
TTACTCAGCC TAAATATCGA GTGCAAAAAA GAGAAATACG ATGTGCAAAT GGGAGGCAGT 
TTATGTCATC CCATGGAGGA TGCATTTGAG AATGACTACA GCAAGGATTC CGCCAATCTG 
GACTGCTGGG ACCTGATTCA AATGCAGGTG GTGGACACGG AACCCGTGAG TCCCAATGCC 
GCATCTCCCA CTCCCTGCAA GGTGTCTGCC ATCCAGTTGC TCCAGCAACA GCAGCAACTG 
CAGCAGCAGC AGCAGCAGCA GCAGAACATT ATCTTGAATG CAGTGCCATT GATTACCATA 
CAGAATAACA AGGAATTGAT GCAACAGCAG CAGCAGCAGC AGCAACAGCA ACAGCAGGAG 
CAGCTGCAGC AGCCGGCTAT AAAACTGCTG AATGGAGCCA GCATTGCGCC GGTGAACACC 
AAGGCCACCA TCCGCCTGGT GGAGAGCAAG CCGCCCACCA CGACGCAGTC TCGAATGGCG 
AAGGTGAACC TCGTGCCGCA GCAGCAGCAG CATGGAAACA AGCGCCACCT GAACAGCGCG 
ACAGGAGCGG GCAATCCCGT GGAGTCCAAG CGTCTGAAGA GCGGAACCCT CTGCCTGGAC 
GTGCAGTCCC CTCAGCTCCT GCAGCAGCTA ATTGGCAAGG ATCCTGCCCA GCAGCAAACC 
CAAGCAGCCA AGCGAGCAGG CAGTGAGCGC TGGCAATTGT CGGCGGAGAG TAAACAGCAG 
AAGCAGCAAC AACAGCAGTC GAACTCGGTA CTAAAGAACC TGCTGGTCAG CGGACGCGAT 
GATGACGATT CCGAAGCAAT GATAATCGAT GAGGACAACT CCCTGGTGCA GCCAATTCCA 
CTGGGCAAAT ATGGCTTGCC CCTGCACTGT CATACCTCCA CGTCTTCGGT GCTGCGGGAC 
TATCATAACA ACCCGCTGAT CAGTGGCACC AACTTCCAGC TGTCTCCAGT CTTCGGAGGC 
TCCGATTCCA GTGGAGGTGA CGGCGAGACT GGGTCAGTGG TTTCCCTAGA TGACTCGGTG 
CCACCCGGCT TGACAGCCTG CGACACGGAT GCCTCCAGCG ACAGTGGCAT CGATGAGAAC 
AGCCTGATGG ATGGGGCATC GGGGTCACCG CGTAAGAGGT TGTCCTCAAC TTCCAATAGC 
ACTAATCAGG CTGAGTCGGC ACCACCGGCT TTGGACGTGG AGACTCCAGT GACCCAGAAG 
TCGGTAGAAG AGGAGTTCGA GGGAGGCGGA AGCGGCAGTA ACGCCCCCAG CAGGAAGACT 
TCCATTTCCT TCCTGGACAG CAGCAATCCC CTGCTGCACA CGCCGGCGAT GATGGACCTG 
GTCAACGACG ACTACATCAT GGGCGAGGGT GGCTTCGAGT TTAGCGACAA CCAGTTGGAG 
CAAGTCCTGG GTTGGCCAGA AATCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sima ( NM_001300670.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287599.1 Drosophila melanogaster similar (sima), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa22178
Clone ID Related Accession (Same CDS sequence)	NM_001300670.1
Accession Version	NM_001300670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	similar, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTAATT CCTTCGAGAG CTGCGATTTT TCGTGTACCG GCGACATTTC GTTCTACGGC 
GAAGATGTGA TCCCCGGTTC TAGTTTCTAC GAGTGCGATT CCAAGCCGCA GCGGCAGCGG 
CAGCTGAGCA GAAATGGCCC AGCTTTTGGG CCAATGCCCA TCAATGAACC CACTGTGAAC 
TTTGGCAACG GGTCATTCTA CAATGGCCCT GCTCCCGGAA GCCAACCCTT CGTGGGCGCT 
ATGCTCGGCC CCAATATGTC CTCCACTGGG ATGCAAAGGT ACCATCCGTA CCATCAGCAG 
CAGCCCCAGG ACGCCTTCTA TCGCCAGAGG GGATCGCTTC TGAACGTGGC CACGCCCTGT 
CGCGAAAGCC GCCCCTGGAG CTACGCCTAC TGCTATGGCT ACGCGCCCAC GAACACTCAA 
CCTTGCCAGT TCTCCCAGTT TGTCGACATC GAGGATTTCA TGAACAATGA AAAACGCAAG 
GAAAAGTCTC GCGATGCGGC TAGATGTCGT CGCTCCAAGG AGACAGAGAT ATTCATGGAA 
CTCTCGGCTG CTTTGCCATT GAAAACCGAC GATGTCAACC AACTGGACAA GGCCTCCGTG 
ATGAGGATCA CCATCGCTTT CCTCAAGATT CGCGAAATGC TGCAGTTCGT GCCCAGCTTG 
CGCGACTGCA ATGATGACAT CAAGCAGGAC ATTGAAACTG CAGAGGATCA GCAGGAAGTC 
AAGCCCAAGC TGGAAGTGGG CACTGAGGAT TGGCTCAACG GAGCTGAGGC CAGAGAGCTC 
CTCAAACAGA CCATGGACGG ATTCCTGTTG GTCTTGTCCC ACGAGGGAGA CATCACCTAC 
GTGTCCGAAA ACGTAGTCGA GTATCTGGGC ATCACCAAGA TTGACACACT TGGCCAACAG 
ATCTGGGAGT ACTCGCACCA GTGCGATCAC GCCGAGATCA AGGAGGCTCT CAGCCTAAAG 
CGTGAACTTG CCCAGAAGGT CAAGGATGAG CCGCAGCAGA ACTCTGGAGT GAGCACCCAT 
CACCGGGATC TGTTTGTGCG CTTGAAGTGC ACCTTGACCA GTCGTGGACG AAGCATCAAC 
ATCAAGAGCG CTTCGTATAA GGTCATTCAC ATCACGGGAC ACTTGGTGGT CAATGCAAAG 
GGCGAACGTC TGCTGATGGC CATTGGCCGA CCAATTCCGC ATCCTTCGAA CATTGAGATT 
CCGTTGGGAA CTAGCACCTT CCTTACCAAA CACTCCCTGG ATATGCGTTT CACCTATGTG 
GATGACAAGA TGCATGACTT ACTGGGATAT TCGCCCAAGG ATCTACTTGA CACCTCTTTG 
TTTTCGTGCC AGCATGGAGC AGATTCCGAA CGCCTGATGG CCACCTTCAA GAGCGTGCTG 
AGCAAGGGAC AGGGCGAGAC CTCCCGCTAC AGATTTCTGG GAAAATACGG TGGCTACTGC 
TGGATTCTTA GCCAGGCCAC GATCGTCTAC GATAAGTTAA AGCCGCAGAG CGTCGTTTGC 
GTTAACTATG TCATAAGTAA TCTTGAGAAT AAACATGAGA TATACTCACT TGCTCAACAA 
ACGGCGGCAA GTGAGCAAAA GGAACAACAC CACCAAGCAG CTGAAACCGA GAAGGAGCCC 
GAAAAAGCAG CCGATCCCGA AATAATCGCT CAGGAAACCA AGGAAACAGT AAACACACCT 
ATTCACACCT CAGAGCTGCA AGCTAAGCCA TTGCAACTTG AGAGCGAGAA AGCAGAGAAA 
ACCATCGAGG AAACCAAAAC AATTGCAACA ATCCCACCAG TAACTGCAAC CTCAACGGCT 
GACCAGATAA AGCAGCTGCC CGAGAGCAAT CCGTACAAGC AGATCCTCCA GGCGGAGTTG 
CTCATCAAGC GGGAGAACCA CTCGCCAGGA CCGCGCACCA TCACTGCCCA GCTGCTCAGC 
GGCAGTAGCA GCGGACTGCG TCCCGAGGAG AAGCGTCCCA AGTCGGTGAC CGCTTCGGTC 
CTCCGCCCGA GTCCAGCTCC ACCCCTAACA CCACCGCCCA CAGCGGTGCT CTGCAAGAAG 
ACTCCGCTCG GCGTGGAGCC CAATCTGCCG CCAACCACCA CCGCTACGGC AGCCATAATC 
TCCTCGAGCA ATCAGCAGCT GCAGATCGCA CAGCAAACCC AACTGCAGAA TCCCCAGCAA 
CCAGCTCAAG ATATGAGTAA AGGATTCTGT TCGCTATTTG CCGACGATGG ACGAGGTCTG 
ACAATGCTTA AGGAAGAACC TGACGATTTG TCCCACCACC TGGCCTCCAC CAATTGCATT 
CAGCTGGACG AGATGACTCC CTTCAGTGAC ATGTTGGTTG GCCTCATGGG CACCTGCCTG 
CTGCCCGAGG ATATCAATTC CCTGGACTCG ACCACCTGCT CCACGACGGC CAGCGGCCAG 
CACTACCAGA GCCCCAGCAG CAGCAGCACC TCTGCACCCA GCAATACCAG TAGCAGCAAT 
AATAGCTATG CCAACAGTCC GCTCAGCCCG CTTACACCCA ATTCCACGGC AACCGCTAGC 
AATCCCAGTC ATCAGCAGCA ACAGCAACAT CACAACCAGC AGCAGCAGCA GCAACAACAG 
CAGCAGCATC ATCCCCAGCA CCACGACAAC AGCAACAGCA GCAGCAACAT CGATCCGCTG 
TTCAACTATC GCGAGGAGTC GAATGACACA AGCTGCTCGC AACATCTGCA TTCGCCTAGC 
ATCACATCAA AGAGTCCCGA GGACAGCAGC CTCCCATCGT TGTGCAGTCC CAATTCACTA 
ACACAGGAGG ATGACTTCTC TTTCGAGGCC TTTGCCATGC GTGCGCCCTA CATCCCGATC 
GATGATGATA TGCCCCTGCT AACGGAGACG GATCTCATGT GGTGCCCACC AGAGGATCTG 
CAGACCATGG TGCCCAAGGA GATCGACGCC ATACAGCAGC AGTTGCAACA GTTGCAGCAG 
CAACATCACC AGCAATACGC CGGCAACACT GGCTACCAGC AGCAGCAGCA GCAGCCGCAA 
CTCCAGCAGC AGCATTTCTC CAACTCCCTG TGCTCCTCGC CAGCTTCAAC GGTCTCGTCA 
TTGTCGCCAT CGCCCGTGCA ACAGCATCAT CAGCAGCAAC AGGCAGCGGT CTTCACCAGC 
GATTCCAGCG AACTGGCGGC ATTGCTGTGC GGATCCGGAA ATGGAACACT TTCGATCCTG 
GCAGGTAGTG GTGTTACGGT TGCGGAGGAG TGCAACGAGC GGCTGCAGCA ACATCAGCAG 
CAGCAACAGC AGACGAGTGG CAACGAGTTC AGGACTTTCC AGCAGCTGCA GCAGGAGTTG 
CAGTTGCAGG AGGAACAGCA GCAGCGTCAG CAGCAGCAAC AACAGCAGCA ACAGCAGCAG 
CAGCAACAGC AGTTACTCAG CCTAAATATC GAGTGCAAAA AAGAGAAATA CGATGTGCAA 
ATGGGAGGCA GTTTATGTCA TCCCATGGAG GATGCATTTG AGAATGACTA CAGCAAGGAT 
TCCGCCAATC TGGACTGCTG GGACCTGATT CAAATGCAGG TGGTGGACAC GGAACCCGTG 
AGTCCCAATG CCGCATCTCC CACTCCCTGC AAGGTGTCTG CCATCCAGTT GCTCCAGCAA 
CAGCAGCAAC TGCAGCAGCA GCAGCAGCAG CAGCAGAACA TTATCTTGAA TGCAGTGCCA 
TTGATTACCA TACAGAATAA CAAGGAATTG ATGCAACAGC AGCAGCAGCA GCAGCAACAG 
CAACAGCAGG AGCAGCTGCA GCAGCCGGCT ATAAAACTGC TGAATGGAGC CAGCATTGCG 
CCGGTGAACA CCAAGGCCAC CATCCGCCTG GTGGAGAGCA AGCCGCCCAC CACGACGCAG 
TCTCGAATGG CGAAGGTGAA CCTCGTGCCG CAGCAGCAGC AGCATGGAAA CAAGCGCCAC 
CTGAACAGCG CGACAGGAGC GGGCAATCCC GTGGAGTCCA AGCGTCTGAA GAGCGGAACC 
CTCTGCCTGG ACGTGCAGTC CCCTCAGCTC CTGCAGCAGC TAATTGGCAA GGATCCTGCC 
CAGCAGCAAA CCCAAGCAGC CAAGCGAGCA GGCAGTGAGC GCTGGCAATT GTCGGCGGAG 
AGTAAACAGC AGAAGCAGCA ACAACAGCAG TCGAACTCGG TACTAAAGAA CCTGCTGGTC 
AGCGGACGCG ATGATGACGA TTCCGAAGCA ATGATAATCG ATGAGGACAA CTCCCTGGTG 
CAGCCAATTC CACTGGGCAA ATATGGCTTG CCCCTGCACT GTCATACCTC CACGTCTTCG 
GTGCTGCGGG ACTATCATAA CAACCCGCTG ATCAGTGGCA CCAACTTCCA GCTGTCTCCA 
GTCTTCGGAG GCTCCGATTC CAGTGGAGGT GACGGCGAGA CTGGGTCAGT GGTTTCCCTA 
GATGACTCGG TGCCACCCGG CTTGACAGCC TGCGACACGG ATGCCTCCAG CGACAGTGGC 
ATCGATGAGA ACAGCCTGAT GGATGGGGCA TCGGGGTCAC CGCGTAAGAG GTTGTCCTCA 
ACTTCCAATA GCACTAATCA GGCTGAGTCG GCACCACCGG CTTTGGACGT GGAGACTCCA 
GTGACCCAGA AGTCGGTAGA AGAGGAGTTC GAGGGAGGCG GAAGCGGCAG TAACGCCCCC 
AGCAGGAAGA CTTCCATTTC CTTCCTGGAC AGCAGCAATC CCCTGCTGCA CACGCCGGCG 
ATGATGGACC TGGTCAACGA CGACTACATC ATGGGCGAGG GTGGCTTCGA GTTTAGCGAC 
AACCAGTTGG AGCAAGTCCT GGGTTGGCCA GAAATCGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287599.1
CDS	157..4938
Translation	MFNSFESCDFSCTGDISFYGEDVIPGSSFYECDSKPQRQRQLSRNGPAFGPMPINEPTVNFGNGSFYNGPAPGSQPFVGAMLGPNMSSTGMQRYHPYHQQQPQDAFYRQRGSLLNVATPCRESRPWSYAYCYGYAPTNTQPCQFSQFVDIEDFMNNEKRKEKSRDAARCRRSKETEIFMELSAALPLKTDDVNQLDKASVMRITIAFLKIREMLQFVPSLRDCNDDIKQDIETAEDQQEVKPKLEVGTEDWLNGAEARELLKQTMDGFLLVLSHEGDITYVSENVVEYLGITKIDTLGQQIWEYSHQCDHAEIKEALSLKRELAQKVKDEPQQNSGVSTHHRDLFVRLKCTLTSRGRSINIKSASYKVIHITGHLVVNAKGERLLMAIGRPIPHPSNIEIPLGTSTFLTKHSLDMRFTYVDDKMHDLLGYSPKDLLDTSLFSCQHGADSERLMATFKSVLSKGQGETSRYRFLGKYGGYCWILSQATIVYDKLKPQSVVCVNYVISNLENKHEIYSLAQQTAASEQKEQHHQAAETEKEPEKAADPEIIAQETKETVNTPIHTSELQAKPLQLESEKAEKTIEETKTIATIPPVTATSTADQIKQLPESNPYKQILQAELLIKRENHSPGPRTITAQLLSGSSSGLRPEEKRPKSVTASVLRPSPAPPLTPPPTAVLCKKTPLGVEPNLPPTTTATAAIISSSNQQLQIAQQTQLQNPQQPAQDMSKGFCSLFADDGRGLTMLKEEPDDLSHHLASTNCIQLDEMTPFSDMLVGLMGTCLLPEDINSLDSTTCSTTASGQHYQSPSSSSTSAPSNTSSSNNSYANSPLSPLTPNSTATASNPSHQQQQQHHNQQQQQQQQQQHHPQHHDNSNSSSNIDPLFNYREESNDTSCSQHLHSPSITSKSPEDSSLPSLCSPNSLTQEDDFSFEAFAMRAPYIPIDDDMPLLTETDLMWCPPEDLQTMVPKEIDAIQQQLQQLQQQHHQQYAGNTGYQQQQQQPQLQQQHFSNSLCSSPASTVSSLSPSPVQQHHQQQQAAVFTSDSSELAALLCGSGNGTLSILAGSGVTVAEECNERLQQHQQQQQQTSGNEFRTFQQLQQELQLQEEQQQRQQQQQQQQQQQQQQQLLSLNIECKKEKYDVQMGGSLCHPMEDAFENDYSKDSANLDCWDLIQMQVVDTEPVSPNAASPTPCKVSAIQLLQQQQQLQQQQQQQQNIILNAVPLITIQNNKELMQQQQQQQQQQQQEQLQQPAIKLLNGASIAPVNTKATIRLVESKPPTTTQSRMAKVNLVPQQQQHGNKRHLNSATGAGNPVESKRLKSGTLCLDVQSPQLLQQLIGKDPAQQQTQAAKRAGSERWQLSAESKQQKQQQQQSNSVLKNLLVSGRDDDDSEAMIIDEDNSLVQPIPLGKYGLPLHCHTSTSSVLRDYHNNPLISGTNFQLSPVFGGSDSSGGDGETGSVVSLDDSVPPGLTACDTDASSDSGIDENSLMDGASGSPRKRLSSTSNSTNQAESAPPALDVETPVTQKSVEEEFEGGGSGSNAPSRKTSISFLDSSNPLLHTPAMMDLVNDDYIMGEGGFEFSDNQLEQVLGWPEIA

Target ORF information:

RefSeq Version	NM_001300670.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster similar (sima), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300670.1

ORF Insert Sequence:

ATGTTTAATT CCTTCGAGAG CTGCGATTTT TCGTGTACCG GCGACATTTC GTTCTACGGC 
GAAGATGTGA TCCCCGGTTC TAGTTTCTAC GAGTGCGATT CCAAGCCGCA GCGGCAGCGG 
CAGCTGAGCA GAAATGGCCC AGCTTTTGGG CCAATGCCCA TCAATGAACC CACTGTGAAC 
TTTGGCAACG GGTCATTCTA CAATGGCCCT GCTCCCGGAA GCCAACCCTT CGTGGGCGCT 
ATGCTCGGCC CCAATATGTC CTCCACTGGG ATGCAAAGGT ACCATCCGTA CCATCAGCAG 
CAGCCCCAGG ACGCCTTCTA TCGCCAGAGG GGATCGCTTC TGAACGTGGC CACGCCCTGT 
CGCGAAAGCC GCCCCTGGAG CTACGCCTAC TGCTATGGCT ACGCGCCCAC GAACACTCAA 
CCTTGCCAGT TCTCCCAGTT TGTCGACATC GAGGATTTCA TGAACAATGA AAAACGCAAG 
GAAAAGTCTC GCGATGCGGC TAGATGTCGT CGCTCCAAGG AGACAGAGAT ATTCATGGAA 
CTCTCGGCTG CTTTGCCATT GAAAACCGAC GATGTCAACC AACTGGACAA GGCCTCCGTG 
ATGAGGATCA CCATCGCTTT CCTCAAGATT CGCGAAATGC TGCAGTTCGT GCCCAGCTTG 
CGCGACTGCA ATGATGACAT CAAGCAGGAC ATTGAAACTG CAGAGGATCA GCAGGAAGTC 
AAGCCCAAGC TGGAAGTGGG CACTGAGGAT TGGCTCAACG GAGCTGAGGC CAGAGAGCTC 
CTCAAACAGA CCATGGACGG ATTCCTGTTG GTCTTGTCCC ACGAGGGAGA CATCACCTAC 
GTGTCCGAAA ACGTAGTCGA GTATCTGGGC ATCACCAAGA TTGACACACT TGGCCAACAG 
ATCTGGGAGT ACTCGCACCA GTGCGATCAC GCCGAGATCA AGGAGGCTCT CAGCCTAAAG 
CGTGAACTTG CCCAGAAGGT CAAGGATGAG CCGCAGCAGA ACTCTGGAGT GAGCACCCAT 
CACCGGGATC TGTTTGTGCG CTTGAAGTGC ACCTTGACCA GTCGTGGACG AAGCATCAAC 
ATCAAGAGCG CTTCGTATAA GGTCATTCAC ATCACGGGAC ACTTGGTGGT CAATGCAAAG 
GGCGAACGTC TGCTGATGGC CATTGGCCGA CCAATTCCGC ATCCTTCGAA CATTGAGATT 
CCGTTGGGAA CTAGCACCTT CCTTACCAAA CACTCCCTGG ATATGCGTTT CACCTATGTG 
GATGACAAGA TGCATGACTT ACTGGGATAT TCGCCCAAGG ATCTACTTGA CACCTCTTTG 
TTTTCGTGCC AGCATGGAGC AGATTCCGAA CGCCTGATGG CCACCTTCAA GAGCGTGCTG 
AGCAAGGGAC AGGGCGAGAC CTCCCGCTAC AGATTTCTGG GAAAATACGG TGGCTACTGC 
TGGATTCTTA GCCAGGCCAC GATCGTCTAC GATAAGTTAA AGCCGCAGAG CGTCGTTTGC 
GTTAACTATG TCATAAGTAA TCTTGAGAAT AAACATGAGA TATACTCACT TGCTCAACAA 
ACGGCGGCAA GTGAGCAAAA GGAACAACAC CACCAAGCAG CTGAAACCGA GAAGGAGCCC 
GAAAAAGCAG CCGATCCCGA AATAATCGCT CAGGAAACCA AGGAAACAGT AAACACACCT 
ATTCACACCT CAGAGCTGCA AGCTAAGCCA TTGCAACTTG AGAGCGAGAA AGCAGAGAAA 
ACCATCGAGG AAACCAAAAC AATTGCAACA ATCCCACCAG TAACTGCAAC CTCAACGGCT 
GACCAGATAA AGCAGCTGCC CGAGAGCAAT CCGTACAAGC AGATCCTCCA GGCGGAGTTG 
CTCATCAAGC GGGAGAACCA CTCGCCAGGA CCGCGCACCA TCACTGCCCA GCTGCTCAGC 
GGCAGTAGCA GCGGACTGCG TCCCGAGGAG AAGCGTCCCA AGTCGGTGAC CGCTTCGGTC 
CTCCGCCCGA GTCCAGCTCC ACCCCTAACA CCACCGCCCA CAGCGGTGCT CTGCAAGAAG 
ACTCCGCTCG GCGTGGAGCC CAATCTGCCG CCAACCACCA CCGCTACGGC AGCCATAATC 
TCCTCGAGCA ATCAGCAGCT GCAGATCGCA CAGCAAACCC AACTGCAGAA TCCCCAGCAA 
CCAGCTCAAG ATATGAGTAA AGGATTCTGT TCGCTATTTG CCGACGATGG ACGAGGTCTG 
ACAATGCTTA AGGAAGAACC TGACGATTTG TCCCACCACC TGGCCTCCAC CAATTGCATT 
CAGCTGGACG AGATGACTCC CTTCAGTGAC ATGTTGGTTG GCCTCATGGG CACCTGCCTG 
CTGCCCGAGG ATATCAATTC CCTGGACTCG ACCACCTGCT CCACGACGGC CAGCGGCCAG 
CACTACCAGA GCCCCAGCAG CAGCAGCACC TCTGCACCCA GCAATACCAG TAGCAGCAAT 
AATAGCTATG CCAACAGTCC GCTCAGCCCG CTTACACCCA ATTCCACGGC AACCGCTAGC 
AATCCCAGTC ATCAGCAGCA ACAGCAACAT CACAACCAGC AGCAGCAGCA GCAACAACAG 
CAGCAGCATC ATCCCCAGCA CCACGACAAC AGCAACAGCA GCAGCAACAT CGATCCGCTG 
TTCAACTATC GCGAGGAGTC GAATGACACA AGCTGCTCGC AACATCTGCA TTCGCCTAGC 
ATCACATCAA AGAGTCCCGA GGACAGCAGC CTCCCATCGT TGTGCAGTCC CAATTCACTA 
ACACAGGAGG ATGACTTCTC TTTCGAGGCC TTTGCCATGC GTGCGCCCTA CATCCCGATC 
GATGATGATA TGCCCCTGCT AACGGAGACG GATCTCATGT GGTGCCCACC AGAGGATCTG 
CAGACCATGG TGCCCAAGGA GATCGACGCC ATACAGCAGC AGTTGCAACA GTTGCAGCAG 
CAACATCACC AGCAATACGC CGGCAACACT GGCTACCAGC AGCAGCAGCA GCAGCCGCAA 
CTCCAGCAGC AGCATTTCTC CAACTCCCTG TGCTCCTCGC CAGCTTCAAC GGTCTCGTCA 
TTGTCGCCAT CGCCCGTGCA ACAGCATCAT CAGCAGCAAC AGGCAGCGGT CTTCACCAGC 
GATTCCAGCG AACTGGCGGC ATTGCTGTGC GGATCCGGAA ATGGAACACT TTCGATCCTG 
GCAGGTAGTG GTGTTACGGT TGCGGAGGAG TGCAACGAGC GGCTGCAGCA ACATCAGCAG 
CAGCAACAGC AGACGAGTGG CAACGAGTTC AGGACTTTCC AGCAGCTGCA GCAGGAGTTG 
CAGTTGCAGG AGGAACAGCA GCAGCGTCAG CAGCAGCAAC AACAGCAGCA ACAGCAGCAG 
CAGCAACAGC AGTTACTCAG CCTAAATATC GAGTGCAAAA AAGAGAAATA CGATGTGCAA 
ATGGGAGGCA GTTTATGTCA TCCCATGGAG GATGCATTTG AGAATGACTA CAGCAAGGAT 
TCCGCCAATC TGGACTGCTG GGACCTGATT CAAATGCAGG TGGTGGACAC GGAACCCGTG 
AGTCCCAATG CCGCATCTCC CACTCCCTGC AAGGTGTCTG CCATCCAGTT GCTCCAGCAA 
CAGCAGCAAC TGCAGCAGCA GCAGCAGCAG CAGCAGAACA TTATCTTGAA TGCAGTGCCA 
TTGATTACCA TACAGAATAA CAAGGAATTG ATGCAACAGC AGCAGCAGCA GCAGCAACAG 
CAACAGCAGG AGCAGCTGCA GCAGCCGGCT ATAAAACTGC TGAATGGAGC CAGCATTGCG 
CCGGTGAACA CCAAGGCCAC CATCCGCCTG GTGGAGAGCA AGCCGCCCAC CACGACGCAG 
TCTCGAATGG CGAAGGTGAA CCTCGTGCCG CAGCAGCAGC AGCATGGAAA CAAGCGCCAC 
CTGAACAGCG CGACAGGAGC GGGCAATCCC GTGGAGTCCA AGCGTCTGAA GAGCGGAACC 
CTCTGCCTGG ACGTGCAGTC CCCTCAGCTC CTGCAGCAGC TAATTGGCAA GGATCCTGCC 
CAGCAGCAAA CCCAAGCAGC CAAGCGAGCA GGCAGTGAGC GCTGGCAATT GTCGGCGGAG 
AGTAAACAGC AGAAGCAGCA ACAACAGCAG TCGAACTCGG TACTAAAGAA CCTGCTGGTC 
AGCGGACGCG ATGATGACGA TTCCGAAGCA ATGATAATCG ATGAGGACAA CTCCCTGGTG 
CAGCCAATTC CACTGGGCAA ATATGGCTTG CCCCTGCACT GTCATACCTC CACGTCTTCG 
GTGCTGCGGG ACTATCATAA CAACCCGCTG ATCAGTGGCA CCAACTTCCA GCTGTCTCCA 
GTCTTCGGAG GCTCCGATTC CAGTGGAGGT GACGGCGAGA CTGGGTCAGT GGTTTCCCTA 
GATGACTCGG TGCCACCCGG CTTGACAGCC TGCGACACGG ATGCCTCCAG CGACAGTGGC 
ATCGATGAGA ACAGCCTGAT GGATGGGGCA TCGGGGTCAC CGCGTAAGAG GTTGTCCTCA 
ACTTCCAATA GCACTAATCA GGCTGAGTCG GCACCACCGG CTTTGGACGT GGAGACTCCA 
GTGACCCAGA AGTCGGTAGA AGAGGAGTTC GAGGGAGGCG GAAGCGGCAG TAACGCCCCC 
AGCAGGAAGA CTTCCATTTC CTTCCTGGAC AGCAGCAATC CCCTGCTGCA CACGCCGGCG 
ATGATGGACC TGGTCAACGA CGACTACATC ATGGGCGAGG GTGGCTTCGA GTTTAGCGAC 
AACCAGTTGG AGCAAGTCCT GGGTTGGCCA GAAATCGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sima cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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