spn-A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spn-A
Entrez Gene ID	43577
Full Name	spindle A
Synonyms	CG7948,D37788,DMR,DMR/DroRAD51,DMR1,Dm Rad51,DmRAD51,DmRad51,Dmel\CG7948,Dmrad51,RA51,RA51_DROME,RAD51,Rad51,Rad51dm,Spn-A,SpnA,dmRAD51,dmrad51,rad51,spnA
General protein information	Preferred Names spindle A Names CG7948 gene product from transcript CG7948-RA CG7948-PA CG7948-PB Rad51-like Spindle-A spn-A-PA spn-A-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99D3-99D3\|3-96 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079844.4	NP_524583.1	spindle A, isoform A
NM_170463.3	NP_733342.1	spindle A, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAD51	NP_002866.2
Pan troglodytes (chimpanzee)	RAD51	XP_001144621.1
Gallus gallus (chicken)	RAD51	NP_990504.1
Danio rerio (zebrafish)	rad51	NP_998371.2
Schizosaccharomyces pombe (fission yeast)	rhp51	NP_593882.1
	LOC708579	XP_001097058.1
Canis lupus familiaris (dog)	RAD51	NP_001003043.1
Caenorhabditis elegans (roundworm)	rad-51	NP_001023465.1
Eremothecium gossypii	AGOS_ACR226W	NP_983628.1
Magnaporthe oryzae (rice blast fungus)	MGG_15576	XP_003713761.1
Arabidopsis thaliana (thale cress)	RAD51	NP_568402.1
Xenopus tropicalis (tropical clawed frog)	rad51	NP_001016393.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013412	XP_003436320.1
Saccharomyces cerevisiae (baker's yeast)	RAD51	NP_011021.3
Kluyveromyces lactis	KLLA0E20241g	XP_454867.1
Bos taurus (cattle)	RAD51	NP_001039644.1
Mus musculus (house mouse)	Rad51	NP_035364.1
Rattus norvegicus (Norway rat)	Rad51	NP_001102674.1
Drosophila melanogaster (fruit fly)	spn-A	NP_524583.1
Neurospora crassa	NCU02741	XP_965126.1
Oryza sativa (rice)	Os11g0615800	NP_001068279.1
Oryza sativa (rice)	Os12g0497300	NP_001066806.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that when both spnA and lig4 were mutated and a circular donor was provided, the frequency of ry mutations was still high and no HR products were recovered.
Title: Genetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila.

Ectopically expressed DmRad51 disrupts cell cycle regulation and induces apoptosis.
Title: Overexpression of Drosophila Rad51 protein (DmRad51) disrupts cell cycle progression and leads to apoptosis.

A conserved N-terminal motif in Rad54 is important for chromatin remodeling and homologous strand pairing. Its autoinhibitory activity is relieved by Rad51.
Title: A conserved N-terminal motif in Rad54 is important for chromatin remodeling and homologous strand pairing.

DmRad51/spnA null mutants are viable but oogenesis is disrupted by the activation of a meiotic recombination checkpoint.
Title: An essential role of DmRad51/SpnA in DNA repair and meiotic checkpoint control.

The following spn-A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spn-A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06606	NM_079844.4 Latest version!	Drosophila melanogaster spindle A (spn-A), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa06607	NM_170463.3 Latest version!	Drosophila melanogaster spindle A (spn-A), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spn-A ( NM_079844.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524583.1 Drosophila melanogaster spindle A (spn-A), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06606
Clone ID Related Accession (Same CDS sequence)	NM_079844.4
Accession Version	NM_079844.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spindle A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079844.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAAGC TAACGAATGT TCAGGCACAG CAGGAAGAGG AGGAGGAAGA AGGTCCACTC 
AGCGTGACTA AGTTAATAGG CGGCAGCATC ACGGCCAAGG ACATCAAGCT GCTCCAGCAG 
GCCAGTCTGC ACACCGTGGA GTCGGTGGCC AATGCCACCA AGAAGCAACT GATGGCCATT 
CCCGGCTTGG GCGGCGGCAA GGTGGAGCAG ATCATCACGG AGGCTAACAA ACTGGTGCCT 
CTGGGCTTCC TTAGTGCTCG CACCTTCTAT CAAATGCGTG CCGATGTCGT GCAGCTGAGC 
ACGGGCTCCA AGGAGCTGGA TAAACTGCTG GGCGGCGGCA TTGAGACGGG ATCCATTACC 
GAGATCTTTG GCGAGTTCCG CTGCGGAAAG ACCCAATTGT GCCACACTCT GGCAGTAACC 
TGCCAGCTGC CCATCAGCCA GAAGGGCGGC GAGGGCAAGT GCATGTACAT CGACACGGAG 
AACACCTTCC GTCCGGAGCG TTTGGCTGCC ATCGCGCAAA GGTACAAACT GAATGAATCC 
GAGGTGCTGG ACAATGTGGC CTTCACCCGT GCCCACAACT CAGATCAGCA GACCAAGCTC 
ATCCAGATGG CGGCGGGCAT GCTCTTTGAG TCCAGATACG CTTTGCTGAT TGTGGACAGT 
GCCATGGCGC TCTACAGATC CGATTATATT GGTCGCGGGG AGCTGGCCGC CAGGCAAAAC 
CATTTGGGCT TATTTCTGCG CATGCTTCAA CGCCTGGCCG ATGAGTTCGG AGTGGCTGTG 
GTAATTACTA ACCAGGTTAC TGCCTCGCTG GACGGCGCAC CCGGCATGTT TGATGCCAAG 
AAGCCCATTG GCGGGCACAT CATGGCCCAC TCCTCAACCA CGCGGCTGTA TCTGCGCAAG 
GGTAAAGGCG AAACCCGCAT CTGCAAGATC TACGACTCGC CCTGTTTGCC GGAATCGGAG 
GCCATGTTCG CCATTCTGCC GGATGGAATA GGAGACGCCA GGGAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524583.1
CDS	99..1109
Translation	MEKLTNVQAQQEEEEEEGPLSVTKLIGGSITAKDIKLLQQASLHTVESVANATKKQLMAIPGLGGGKVEQIITEANKLVPLGFLSARTFYQMRADVVQLSTGSKELDKLLGGGIETGSITEIFGEFRCGKTQLCHTLAVTCQLPISQKGGEGKCMYIDTENTFRPERLAAIAQRYKLNESEVLDNVAFTRAHNSDQQTKLIQMAAGMLFESRYALLIVDSAMALYRSDYIGRGELAARQNHLGLFLRMLQRLADEFGVAVVITNQVTASLDGAPGMFDAKKPIGGHIMAHSSTTRLYLRKGKGETRICKIYDSPCLPESEAMFAILPDGIGDARES

Target ORF information:

RefSeq Version	NM_079844.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spindle A (spn-A), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079844.4

ORF Insert Sequence:

ATGGAGAAGC TAACGAATGT TCAGGCACAG CAGGAAGAGG AGGAGGAAGA AGGTCCACTC 
AGCGTGACTA AGTTAATAGG CGGCAGCATC ACGGCCAAGG ACATCAAGCT GCTCCAGCAG 
GCCAGTCTGC ACACCGTGGA GTCGGTGGCC AATGCCACCA AGAAGCAACT GATGGCCATT 
CCCGGCTTGG GCGGCGGCAA GGTGGAGCAG ATCATCACGG AGGCTAACAA ACTGGTGCCT 
CTGGGCTTCC TTAGTGCTCG CACCTTCTAT CAAATGCGTG CCGATGTCGT GCAGCTGAGC 
ACGGGCTCCA AGGAGCTGGA TAAACTGCTG GGCGGCGGCA TTGAGACGGG ATCCATTACC 
GAGATCTTTG GCGAGTTCCG CTGCGGAAAG ACCCAATTGT GCCACACTCT GGCAGTAACC 
TGCCAGCTGC CCATCAGCCA GAAGGGCGGC GAGGGCAAGT GCATGTACAT CGACACGGAG 
AACACCTTCC GTCCGGAGCG TTTGGCTGCC ATCGCGCAAA GGTACAAACT GAATGAATCC 
GAGGTGCTGG ACAATGTGGC CTTCACCCGT GCCCACAACT CAGATCAGCA GACCAAGCTC 
ATCCAGATGG CGGCGGGCAT GCTCTTTGAG TCCAGATACG CTTTGCTGAT TGTGGACAGT 
GCCATGGCGC TCTACAGATC CGATTATATT GGTCGCGGGG AGCTGGCCGC CAGGCAAAAC 
CATTTGGGCT TATTTCTGCG CATGCTTCAA CGCCTGGCCG ATGAGTTCGG AGTGGCTGTG 
GTAATTACTA ACCAGGTTAC TGCCTCGCTG GACGGCGCAC CCGGCATGTT TGATGCCAAG 
AAGCCCATTG GCGGGCACAT CATGGCCCAC TCCTCAACCA CGCGGCTGTA TCTGCGCAAG 
GGTAAAGGCG AAACCCGCAT CTGCAAGATC TACGACTCGC CCTGTTTGCC GGAATCGGAG 
GCCATGTTCG CCATTCTGCC GGATGGAATA GGAGACGCCA GGGAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spn-A ( NM_170463.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733342.1 Drosophila melanogaster spindle A (spn-A), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06607
Clone ID Related Accession (Same CDS sequence)	NM_170463.3
Accession Version	NM_170463.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spindle A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170463.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATTC CCGGCTTGGG CGGCGGCAAG GTGGAGCAGA TCATCACGGA GGCTAACAAA 
CTGGTGCCTC TGGGCTTCCT TAGTGCTCGC ACCTTCTATC AAATGCGTGC CGATGTCGTG 
CAGCTGAGCA CGGGCTCCAA GGAGCTGGAT AAACTGCTGG GCGGCGGCAT TGAGACGGGA 
TCCATTACCG AGATCTTTGG CGAGTTCCGC TGCGGAAAGA CCCAATTGTG CCACACTCTG 
GCAGTAACCT GCCAGCTGCC CATCAGCCAG AAGGGCGGCG AGGGCAAGTG CATGTACATC 
GACACGGAGA ACACCTTCCG TCCGGAGCGT TTGGCTGCCA TCGCGCAAAG GTACAAACTG 
AATGAATCCG AGGTGCTGGA CAATGTGGCC TTCACCCGTG CCCACAACTC AGATCAGCAG 
ACCAAGCTCA TCCAGATGGC GGCGGGCATG CTCTTTGAGT CCAGATACGC TTTGCTGATT 
GTGGACAGTG CCATGGCGCT CTACAGATCC GATTATATTG GTCGCGGGGA GCTGGCCGCC 
AGGCAAAACC ATTTGGGCTT ATTTCTGCGC ATGCTTCAAC GCCTGGCCGA TGAGTTCGGA 
GTGGCTGTGG TAATTACTAA CCAGGTTACT GCCTCGCTGG ACGGCGCACC CGGCATGTTT 
GATGCCAAGA AGCCCATTGG CGGGCACATC ATGGCCCACT CCTCAACCAC GCGGCTGTAT 
CTGCGCAAGG GTAAAGGCGA AACCCGCATC TGCAAGATCT ACGACTCGCC CTGTTTGCCG 
GAATCGGAGG CCATGTTCGC CATTCTGCCG GATGGAATAG GAGACGCCAG GGAGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733342.1
CDS	333..1172
Translation	MAIPGLGGGKVEQIITEANKLVPLGFLSARTFYQMRADVVQLSTGSKELDKLLGGGIETGSITEIFGEFRCGKTQLCHTLAVTCQLPISQKGGEGKCMYIDTENTFRPERLAAIAQRYKLNESEVLDNVAFTRAHNSDQQTKLIQMAAGMLFESRYALLIVDSAMALYRSDYIGRGELAARQNHLGLFLRMLQRLADEFGVAVVITNQVTASLDGAPGMFDAKKPIGGHIMAHSSTTRLYLRKGKGETRICKIYDSPCLPESEAMFAILPDGIGDARES

Target ORF information:

RefSeq Version	NM_170463.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spindle A (spn-A), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170463.3

ORF Insert Sequence:

ATGGCCATTC CCGGCTTGGG CGGCGGCAAG GTGGAGCAGA TCATCACGGA GGCTAACAAA 
CTGGTGCCTC TGGGCTTCCT TAGTGCTCGC ACCTTCTATC AAATGCGTGC CGATGTCGTG 
CAGCTGAGCA CGGGCTCCAA GGAGCTGGAT AAACTGCTGG GCGGCGGCAT TGAGACGGGA 
TCCATTACCG AGATCTTTGG CGAGTTCCGC TGCGGAAAGA CCCAATTGTG CCACACTCTG 
GCAGTAACCT GCCAGCTGCC CATCAGCCAG AAGGGCGGCG AGGGCAAGTG CATGTACATC 
GACACGGAGA ACACCTTCCG TCCGGAGCGT TTGGCTGCCA TCGCGCAAAG GTACAAACTG 
AATGAATCCG AGGTGCTGGA CAATGTGGCC TTCACCCGTG CCCACAACTC AGATCAGCAG 
ACCAAGCTCA TCCAGATGGC GGCGGGCATG CTCTTTGAGT CCAGATACGC TTTGCTGATT 
GTGGACAGTG CCATGGCGCT CTACAGATCC GATTATATTG GTCGCGGGGA GCTGGCCGCC 
AGGCAAAACC ATTTGGGCTT ATTTCTGCGC ATGCTTCAAC GCCTGGCCGA TGAGTTCGGA 
GTGGCTGTGG TAATTACTAA CCAGGTTACT GCCTCGCTGG ACGGCGCACC CGGCATGTTT 
GATGCCAAGA AGCCCATTGG CGGGCACATC ATGGCCCACT CCTCAACCAC GCGGCTGTAT 
CTGCGCAAGG GTAAAGGCGA AACCCGCATC TGCAAGATCT ACGACTCGCC CTGTTTGCCG 
GAATCGGAGG CCATGTTCGC CATTCTGCCG GATGGAATAG GAGACGCCAG GGAGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spn-A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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