ncd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ncd
Entrez Gene ID	43517
Full Name	non-claret disjunctional
Synonyms	CG7831,DmNCD,DmNcd,Dmel\CG7831,KIF C1,KIFC1,NCD,Ncd,ca,ca[nd],cd,l(3)05884,ndc
General protein information	Preferred Names non-claret disjunctional Names CG7831 gene product from transcript CG7831-RB CG7831-PA CG7831-PB Kinesin3A Non-Claret disjunctional claret nondisjunctional kinesin-14 ncd-PA ncd-PB non-claret disjunction non-claret-disjunctional nonclaret disjunction nonclaret disjunctional
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99C1-99C1\|3-100.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057303.5	NP_476651.1	non-claret disjunctional, isoform A
NM_001300663.1	NP_001287592.1	non-claret disjunctional, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIFC1	NP_002254.2
	KIFC1	XP_001109683.1
Canis lupus familiaris (dog)	KIFC1	XP_005627265.1
Rattus norvegicus (Norway rat)	Kifc1	NP_001005878.1
Gallus gallus (chicken)	LOC101748559	XP_004945725.1
Magnaporthe oryzae (rice blast fungus)	MGG_05350	XP_003710188.1
Oryza sativa (rice)	Os04g0629700	NP_001053970.1
Mus musculus (house mouse)	Kifc1	NP_001182227.1
Mus musculus (house mouse)	Kifc5b	NP_444403.2
Drosophila melanogaster (fruit fly)	ncd	NP_476651.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002248	XP_307936.4
Arabidopsis thaliana (thale cress)	ATK5	NP_192428.2
Arabidopsis thaliana (thale cress)	ATK2	NP_567768.1
Neurospora crassa	NCU04581	XP_958282.1
Arabidopsis thaliana (thale cress)	ATK3	NP_568811.1
Pan troglodytes (chimpanzee)	KIFC1	XP_518406.4
Bos taurus (cattle)	KIFC1	NP_001095406.1
Gallus gallus (chicken)	KIFC1	NP_001075167.1
Danio rerio (zebrafish)	kifc1	NP_571281.1
Arabidopsis thaliana (thale cress)	ATK1	NP_193859.1
Xenopus tropicalis (tropical clawed frog)	kifc1	XP_002938711.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

14-3-3 translates a spatial cue provided by Aurora B to target Ncd selectively to the spindle within the large volume of oocytes.
Title: 14-3-3 regulation of Ncd reveals a new mechanism for targeting proteins to the spindle in oocytes.

tip-tracking of kinesin-14 occurs through several concurrent mechanisms: binding of kinesin only to EB1 located at the MT end, interaction of the kinesin molecules with a composite site formed by EB1 and the MT tip, and probably surface diffusion of the tail along MT.
Title: Insights into the process of EB1-dependent tip-tracking of kinesin-14 Ncd. The role of the microtubule.

Drosophila Ncd reveals an evolutionarily conserved powerstroke mechanism for homodimeric and heterodimeric kinesin-14s.
Title: Drosophila Ncd reveals an evolutionarily conserved powerstroke mechanism for homodimeric and heterodimeric kinesin-14s.

Data suggest that the C-terminal residues of Ncd play an important role in modulating the interaction of the motor with the microtubule.
Title: The C-terminus of kinesin-14 Ncd is a crucial component of the force generating mechanism.

Mutation of an invariant residue in loop L7 of the central beta-sheet of the Drosophila kinesin-14 Ncd motor alters both nucleotide and microtubule binding, although the mutated residue is not present in either site.
Title: Altered nucleotide-microtubule coupling and increased mechanical output by a kinesin mutant.

The following ncd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ncd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06546	NM_057303.5 Latest version!	Drosophila melanogaster non-claret disjunctional (ncd), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06546	NM_001300663.1 Latest version!	Drosophila melanogaster non-claret disjunctional (ncd), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ncd ( NM_057303.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476651.1 Drosophila melanogaster non-claret disjunctional (ncd), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06546
Clone ID Related Accession (Same CDS sequence)	NM_057303.5 , NM_001300663.1
Accession Version	NM_057303.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	non-claret disjunctional, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057303.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATCCC GGCTACCGAA ACCGTCGGGC CTGAAGAAAC CCCAAATGCC GATTAAAACC 
GTGCTGCCCA CAGATCGAAT TCGCGCAGGA TTGGGAGGTG GAGCCGCTGG AGCAGGCGCC 
TTCAATGTCA ATGCCAACCA GACATACTGC GGCAACTTAT TGCCGCCCCT CTCAAGGGAC 
CTCAACAATC TGCCCCAGGT GCTGGAGCGT CGCGGAGGAG GAGCACGTGC CGCCTCCCCA 
GAGCCCATGA AGTTGGGCCA CCGGGCCAAG CTGAGACGTA GCCGTAGCGC TTGCGACATC 
AACGAACTGC GTGGTAACAA GCGCACTGCG GCTGCTCCTT CATTGCCCAG CATTCCCAGC 
AAAGTATCCC GCCTGGGCGG TGCACTCACT GTTTCCAGCC AGCGACTAGT GCGTCCTGCG 
GCGCCTTCGT CAATAACAGC AACAGCTGTC AAAAGACCAC CAGTAACGCG TCCTGCTCCA 
CGGGCTGCAG GAGGAGCAGC CGCCAAGAAA CCAGCAGGAA CAGGAGCAGC AGCTTCGTCA 
GGAGCCGCGG CTGCTGCTCC CAAGCGCATC GCTCCCTACG ACTTCAAGGC CCGCTTCCAC 
GATCTGCTAG AGAAGCACAA GGTGCTTAAG ACAAAGTACG AAAAGCAAAC AGAGGACATG 
GGCGAGCTGG AGTCCATGCC TCAGCAACTG GAGGAGACGC AGAACAAGCT TATCGAGACG 
GAGTCCTCGC TGAAGAACAC CCAGAGCGAC AACGAGTGTC TTCAGAGGCA GGTGAAGCAG 
CATACCGCCA AAATTGAAAC AATCACATCG ACGCTGGGCA GGACCAAAGA GGAGCTATCC 
GAGCTGCAAG CAATACATGA GAAAGTAAAA ACGGAGCATG CTGCTCTAAG CACAGAAGTG 
GTGCATCTGC GCCAGCGCAC CGAGGAACTC CTGCGCTGCA ATGAGCAGCA GGCCGCCGAG 
CTGGAGACCT GCAAAGAGCA GCTCTTCCAG TCGAACATGG AGCGCAAAGA GCTGCACAAC 
ACGGTCATGG ACCTGCGCGG CAACATCCGG GTCTTCTGTC GAATACGACC GCCGCTGGAG 
TCCGAGGAGA ACCGTATGTG TTGCACCTGG ACCTATCACG ACGAGTCCAC CGTGGAGCTG 
CAGAGCATTG ACGCACAGGC CAAAAGCAAG ATGGGCCAGC AGATCTTCTC ATTCGACCAG 
GTCTTCCACC CGCTCTCCTC GCAGTCGGAC ATCTTCGAGA TGGTCTCGCC GCTCATCCAG 
TCGGCCCTGG ATGGCTACAA TATCTGCATC TTTGCCTACG GACAGACGGG CAGTGGCAAG 
ACCTACACAA TGGACGGAGT GCCGGAGAGT GTGGGCGTCA TACCGCGCAC GGTGGATCTG 
CTCTTCGACT CCATCCGGGG ATATCGCAAC TTGGGCTGGG AGTACGAGAT CAAGGCCACC 
TTTCTGGAGA TCTACAACGA GGTGCTCTAC GATCTGCTGA GCAACGAGCA GAAGGACATG 
GAGATTCGAA TGGCCAAGAA CAACAAGAAC GACATCTACG TGTCCAACAT AACGGAGGAG 
ACGGTTCTGG ATCCAAATCA CCTGCGCCAC CTCATGCACA CGGCCAAGAT GAACCGTGCC 
ACCGCCTCGA CAGCTGGCAA CGAGCGCTCC TCTCGTTCCC ACGCGGTTAC CAAGCTTGAG 
CTCATCGGAC GCCATGCCGA AAAGCAAGAG ATCTCCGTGG GTTCCATAAA CCTGGTGGAT 
TTGGCCGGCT CTGAGTCTCC CAAGACGAGC ACCCGGATGA CCGAGACAAA GAACATCAAT 
CGCTCGCTAT CGGAGCTCAC CAACGTAATC CTGGCGCTGC TGCAGAAGCA GGACCACATC 
CCGTACAGGA ACTCCAAGCT GACGCACCTG CTGATGCCCT CGCTGGGCGG CAACTCGAAA 
ACGCTTATGT TCATCAACGT CTCGCCGTTC CAAGACTGTT TCCAAGAGTC CGTCAAGTCG 
CTGCGCTTCG CGGCCTCCGT AAACTCCTGC AAAATGACCA AGGCCAAGCG GAATCGCTAC 
CTGAACAACT CGGTGGCCAA CAGCAGCACA CAGAGCAACA ACAGCGGCAG TTTCGATAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476651.1
CDS	122..2224
Translation	MESRLPKPSGLKKPQMPIKTVLPTDRIRAGLGGGAAGAGAFNVNANQTYCGNLLPPLSRDLNNLPQVLERRGGGARAASPEPMKLGHRAKLRRSRSACDINELRGNKRTAAAPSLPSIPSKVSRLGGALTVSSQRLVRPAAPSSITATAVKRPPVTRPAPRAAGGAAAKKPAGTGAAASSGAAAAAPKRIAPYDFKARFHDLLEKHKVLKTKYEKQTEDMGELESMPQQLEETQNKLIETESSLKNTQSDNECLQRQVKQHTAKIETITSTLGRTKEELSELQAIHEKVKTEHAALSTEVVHLRQRTEELLRCNEQQAAELETCKEQLFQSNMERKELHNTVMDLRGNIRVFCRIRPPLESEENRMCCTWTYHDESTVELQSIDAQAKSKMGQQIFSFDQVFHPLSSQSDIFEMVSPLIQSALDGYNICIFAYGQTGSGKTYTMDGVPESVGVIPRTVDLLFDSIRGYRNLGWEYEIKATFLEIYNEVLYDLLSNEQKDMEIRMAKNNKNDIYVSNITEETVLDPNHLRHLMHTAKMNRATASTAGNERSSRSHAVTKLELIGRHAEKQEISVGSINLVDLAGSESPKTSTRMTETKNINRSLSELTNVILALLQKQDHIPYRNSKLTHLLMPSLGGNSKTLMFINVSPFQDCFQESVKSLRFAASVNSCKMTKAKRNRYLNNSVANSSTQSNNSGSFDK

Target ORF information:

RefSeq Version	NM_057303.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster non-claret disjunctional (ncd), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057303.5

ORF Insert Sequence:

ATGGAATCCC GGCTACCGAA ACCGTCGGGC CTGAAGAAAC CCCAAATGCC GATTAAAACC 
GTGCTGCCCA CAGATCGAAT TCGCGCAGGA TTGGGAGGTG GAGCCGCTGG AGCAGGCGCC 
TTCAATGTCA ATGCCAACCA GACATACTGC GGCAACTTAT TGCCGCCCCT CTCAAGGGAC 
CTCAACAATC TGCCCCAGGT GCTGGAGCGT CGCGGAGGAG GAGCACGTGC CGCCTCCCCA 
GAGCCCATGA AGTTGGGCCA CCGGGCCAAG CTGAGACGTA GCCGTAGCGC TTGCGACATC 
AACGAACTGC GTGGTAACAA GCGCACTGCG GCTGCTCCTT CATTGCCCAG CATTCCCAGC 
AAAGTATCCC GCCTGGGCGG TGCACTCACT GTTTCCAGCC AGCGACTAGT GCGTCCTGCG 
GCGCCTTCGT CAATAACAGC AACAGCTGTC AAAAGACCAC CAGTAACGCG TCCTGCTCCA 
CGGGCTGCAG GAGGAGCAGC CGCCAAGAAA CCAGCAGGAA CAGGAGCAGC AGCTTCGTCA 
GGAGCCGCGG CTGCTGCTCC CAAGCGCATC GCTCCCTACG ACTTCAAGGC CCGCTTCCAC 
GATCTGCTAG AGAAGCACAA GGTGCTTAAG ACAAAGTACG AAAAGCAAAC AGAGGACATG 
GGCGAGCTGG AGTCCATGCC TCAGCAACTG GAGGAGACGC AGAACAAGCT TATCGAGACG 
GAGTCCTCGC TGAAGAACAC CCAGAGCGAC AACGAGTGTC TTCAGAGGCA GGTGAAGCAG 
CATACCGCCA AAATTGAAAC AATCACATCG ACGCTGGGCA GGACCAAAGA GGAGCTATCC 
GAGCTGCAAG CAATACATGA GAAAGTAAAA ACGGAGCATG CTGCTCTAAG CACAGAAGTG 
GTGCATCTGC GCCAGCGCAC CGAGGAACTC CTGCGCTGCA ATGAGCAGCA GGCCGCCGAG 
CTGGAGACCT GCAAAGAGCA GCTCTTCCAG TCGAACATGG AGCGCAAAGA GCTGCACAAC 
ACGGTCATGG ACCTGCGCGG CAACATCCGG GTCTTCTGTC GAATACGACC GCCGCTGGAG 
TCCGAGGAGA ACCGTATGTG TTGCACCTGG ACCTATCACG ACGAGTCCAC CGTGGAGCTG 
CAGAGCATTG ACGCACAGGC CAAAAGCAAG ATGGGCCAGC AGATCTTCTC ATTCGACCAG 
GTCTTCCACC CGCTCTCCTC GCAGTCGGAC ATCTTCGAGA TGGTCTCGCC GCTCATCCAG 
TCGGCCCTGG ATGGCTACAA TATCTGCATC TTTGCCTACG GACAGACGGG CAGTGGCAAG 
ACCTACACAA TGGACGGAGT GCCGGAGAGT GTGGGCGTCA TACCGCGCAC GGTGGATCTG 
CTCTTCGACT CCATCCGGGG ATATCGCAAC TTGGGCTGGG AGTACGAGAT CAAGGCCACC 
TTTCTGGAGA TCTACAACGA GGTGCTCTAC GATCTGCTGA GCAACGAGCA GAAGGACATG 
GAGATTCGAA TGGCCAAGAA CAACAAGAAC GACATCTACG TGTCCAACAT AACGGAGGAG 
ACGGTTCTGG ATCCAAATCA CCTGCGCCAC CTCATGCACA CGGCCAAGAT GAACCGTGCC 
ACCGCCTCGA CAGCTGGCAA CGAGCGCTCC TCTCGTTCCC ACGCGGTTAC CAAGCTTGAG 
CTCATCGGAC GCCATGCCGA AAAGCAAGAG ATCTCCGTGG GTTCCATAAA CCTGGTGGAT 
TTGGCCGGCT CTGAGTCTCC CAAGACGAGC ACCCGGATGA CCGAGACAAA GAACATCAAT 
CGCTCGCTAT CGGAGCTCAC CAACGTAATC CTGGCGCTGC TGCAGAAGCA GGACCACATC 
CCGTACAGGA ACTCCAAGCT GACGCACCTG CTGATGCCCT CGCTGGGCGG CAACTCGAAA 
ACGCTTATGT TCATCAACGT CTCGCCGTTC CAAGACTGTT TCCAAGAGTC CGTCAAGTCG 
CTGCGCTTCG CGGCCTCCGT AAACTCCTGC AAAATGACCA AGGCCAAGCG GAATCGCTAC 
CTGAACAACT CGGTGGCCAA CAGCAGCACA CAGAGCAACA ACAGCGGCAG TTTCGATAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncd ( NM_001300663.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287592.1 Drosophila melanogaster non-claret disjunctional (ncd), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06546
Clone ID Related Accession (Same CDS sequence)	NM_057303.5 , NM_001300663.1
Accession Version	NM_001300663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	non-claret disjunctional, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATCCC GGCTACCGAA ACCGTCGGGC CTGAAGAAAC CCCAAATGCC GATTAAAACC 
GTGCTGCCCA CAGATCGAAT TCGCGCAGGA TTGGGAGGTG GAGCCGCTGG AGCAGGCGCC 
TTCAATGTCA ATGCCAACCA GACATACTGC GGCAACTTAT TGCCGCCCCT CTCAAGGGAC 
CTCAACAATC TGCCCCAGGT GCTGGAGCGT CGCGGAGGAG GAGCACGTGC CGCCTCCCCA 
GAGCCCATGA AGTTGGGCCA CCGGGCCAAG CTGAGACGTA GCCGTAGCGC TTGCGACATC 
AACGAACTGC GTGGTAACAA GCGCACTGCG GCTGCTCCTT CATTGCCCAG CATTCCCAGC 
AAAGTATCCC GCCTGGGCGG TGCACTCACT GTTTCCAGCC AGCGACTAGT GCGTCCTGCG 
GCGCCTTCGT CAATAACAGC AACAGCTGTC AAAAGACCAC CAGTAACGCG TCCTGCTCCA 
CGGGCTGCAG GAGGAGCAGC CGCCAAGAAA CCAGCAGGAA CAGGAGCAGC AGCTTCGTCA 
GGAGCCGCGG CTGCTGCTCC CAAGCGCATC GCTCCCTACG ACTTCAAGGC CCGCTTCCAC 
GATCTGCTAG AGAAGCACAA GGTGCTTAAG ACAAAGTACG AAAAGCAAAC AGAGGACATG 
GGCGAGCTGG AGTCCATGCC TCAGCAACTG GAGGAGACGC AGAACAAGCT TATCGAGACG 
GAGTCCTCGC TGAAGAACAC CCAGAGCGAC AACGAGTGTC TTCAGAGGCA GGTGAAGCAG 
CATACCGCCA AAATTGAAAC AATCACATCG ACGCTGGGCA GGACCAAAGA GGAGCTATCC 
GAGCTGCAAG CAATACATGA GAAAGTAAAA ACGGAGCATG CTGCTCTAAG CACAGAAGTG 
GTGCATCTGC GCCAGCGCAC CGAGGAACTC CTGCGCTGCA ATGAGCAGCA GGCCGCCGAG 
CTGGAGACCT GCAAAGAGCA GCTCTTCCAG TCGAACATGG AGCGCAAAGA GCTGCACAAC 
ACGGTCATGG ACCTGCGCGG CAACATCCGG GTCTTCTGTC GAATACGACC GCCGCTGGAG 
TCCGAGGAGA ACCGTATGTG TTGCACCTGG ACCTATCACG ACGAGTCCAC CGTGGAGCTG 
CAGAGCATTG ACGCACAGGC CAAAAGCAAG ATGGGCCAGC AGATCTTCTC ATTCGACCAG 
GTCTTCCACC CGCTCTCCTC GCAGTCGGAC ATCTTCGAGA TGGTCTCGCC GCTCATCCAG 
TCGGCCCTGG ATGGCTACAA TATCTGCATC TTTGCCTACG GACAGACGGG CAGTGGCAAG 
ACCTACACAA TGGACGGAGT GCCGGAGAGT GTGGGCGTCA TACCGCGCAC GGTGGATCTG 
CTCTTCGACT CCATCCGGGG ATATCGCAAC TTGGGCTGGG AGTACGAGAT CAAGGCCACC 
TTTCTGGAGA TCTACAACGA GGTGCTCTAC GATCTGCTGA GCAACGAGCA GAAGGACATG 
GAGATTCGAA TGGCCAAGAA CAACAAGAAC GACATCTACG TGTCCAACAT AACGGAGGAG 
ACGGTTCTGG ATCCAAATCA CCTGCGCCAC CTCATGCACA CGGCCAAGAT GAACCGTGCC 
ACCGCCTCGA CAGCTGGCAA CGAGCGCTCC TCTCGTTCCC ACGCGGTTAC CAAGCTTGAG 
CTCATCGGAC GCCATGCCGA AAAGCAAGAG ATCTCCGTGG GTTCCATAAA CCTGGTGGAT 
TTGGCCGGCT CTGAGTCTCC CAAGACGAGC ACCCGGATGA CCGAGACAAA GAACATCAAT 
CGCTCGCTAT CGGAGCTCAC CAACGTAATC CTGGCGCTGC TGCAGAAGCA GGACCACATC 
CCGTACAGGA ACTCCAAGCT GACGCACCTG CTGATGCCCT CGCTGGGCGG CAACTCGAAA 
ACGCTTATGT TCATCAACGT CTCGCCGTTC CAAGACTGTT TCCAAGAGTC CGTCAAGTCG 
CTGCGCTTCG CGGCCTCCGT AAACTCCTGC AAAATGACCA AGGCCAAGCG GAATCGCTAC 
CTGAACAACT CGGTGGCCAA CAGCAGCACA CAGAGCAACA ACAGCGGCAG TTTCGATAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287592.1
CDS	502..2604
Translation	MESRLPKPSGLKKPQMPIKTVLPTDRIRAGLGGGAAGAGAFNVNANQTYCGNLLPPLSRDLNNLPQVLERRGGGARAASPEPMKLGHRAKLRRSRSACDINELRGNKRTAAAPSLPSIPSKVSRLGGALTVSSQRLVRPAAPSSITATAVKRPPVTRPAPRAAGGAAAKKPAGTGAAASSGAAAAAPKRIAPYDFKARFHDLLEKHKVLKTKYEKQTEDMGELESMPQQLEETQNKLIETESSLKNTQSDNECLQRQVKQHTAKIETITSTLGRTKEELSELQAIHEKVKTEHAALSTEVVHLRQRTEELLRCNEQQAAELETCKEQLFQSNMERKELHNTVMDLRGNIRVFCRIRPPLESEENRMCCTWTYHDESTVELQSIDAQAKSKMGQQIFSFDQVFHPLSSQSDIFEMVSPLIQSALDGYNICIFAYGQTGSGKTYTMDGVPESVGVIPRTVDLLFDSIRGYRNLGWEYEIKATFLEIYNEVLYDLLSNEQKDMEIRMAKNNKNDIYVSNITEETVLDPNHLRHLMHTAKMNRATASTAGNERSSRSHAVTKLELIGRHAEKQEISVGSINLVDLAGSESPKTSTRMTETKNINRSLSELTNVILALLQKQDHIPYRNSKLTHLLMPSLGGNSKTLMFINVSPFQDCFQESVKSLRFAASVNSCKMTKAKRNRYLNNSVANSSTQSNNSGSFDK

Target ORF information:

RefSeq Version	NM_001300663.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster non-claret disjunctional (ncd), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300663.1

ORF Insert Sequence:

ATGGAATCCC GGCTACCGAA ACCGTCGGGC CTGAAGAAAC CCCAAATGCC GATTAAAACC 
GTGCTGCCCA CAGATCGAAT TCGCGCAGGA TTGGGAGGTG GAGCCGCTGG AGCAGGCGCC 
TTCAATGTCA ATGCCAACCA GACATACTGC GGCAACTTAT TGCCGCCCCT CTCAAGGGAC 
CTCAACAATC TGCCCCAGGT GCTGGAGCGT CGCGGAGGAG GAGCACGTGC CGCCTCCCCA 
GAGCCCATGA AGTTGGGCCA CCGGGCCAAG CTGAGACGTA GCCGTAGCGC TTGCGACATC 
AACGAACTGC GTGGTAACAA GCGCACTGCG GCTGCTCCTT CATTGCCCAG CATTCCCAGC 
AAAGTATCCC GCCTGGGCGG TGCACTCACT GTTTCCAGCC AGCGACTAGT GCGTCCTGCG 
GCGCCTTCGT CAATAACAGC AACAGCTGTC AAAAGACCAC CAGTAACGCG TCCTGCTCCA 
CGGGCTGCAG GAGGAGCAGC CGCCAAGAAA CCAGCAGGAA CAGGAGCAGC AGCTTCGTCA 
GGAGCCGCGG CTGCTGCTCC CAAGCGCATC GCTCCCTACG ACTTCAAGGC CCGCTTCCAC 
GATCTGCTAG AGAAGCACAA GGTGCTTAAG ACAAAGTACG AAAAGCAAAC AGAGGACATG 
GGCGAGCTGG AGTCCATGCC TCAGCAACTG GAGGAGACGC AGAACAAGCT TATCGAGACG 
GAGTCCTCGC TGAAGAACAC CCAGAGCGAC AACGAGTGTC TTCAGAGGCA GGTGAAGCAG 
CATACCGCCA AAATTGAAAC AATCACATCG ACGCTGGGCA GGACCAAAGA GGAGCTATCC 
GAGCTGCAAG CAATACATGA GAAAGTAAAA ACGGAGCATG CTGCTCTAAG CACAGAAGTG 
GTGCATCTGC GCCAGCGCAC CGAGGAACTC CTGCGCTGCA ATGAGCAGCA GGCCGCCGAG 
CTGGAGACCT GCAAAGAGCA GCTCTTCCAG TCGAACATGG AGCGCAAAGA GCTGCACAAC 
ACGGTCATGG ACCTGCGCGG CAACATCCGG GTCTTCTGTC GAATACGACC GCCGCTGGAG 
TCCGAGGAGA ACCGTATGTG TTGCACCTGG ACCTATCACG ACGAGTCCAC CGTGGAGCTG 
CAGAGCATTG ACGCACAGGC CAAAAGCAAG ATGGGCCAGC AGATCTTCTC ATTCGACCAG 
GTCTTCCACC CGCTCTCCTC GCAGTCGGAC ATCTTCGAGA TGGTCTCGCC GCTCATCCAG 
TCGGCCCTGG ATGGCTACAA TATCTGCATC TTTGCCTACG GACAGACGGG CAGTGGCAAG 
ACCTACACAA TGGACGGAGT GCCGGAGAGT GTGGGCGTCA TACCGCGCAC GGTGGATCTG 
CTCTTCGACT CCATCCGGGG ATATCGCAAC TTGGGCTGGG AGTACGAGAT CAAGGCCACC 
TTTCTGGAGA TCTACAACGA GGTGCTCTAC GATCTGCTGA GCAACGAGCA GAAGGACATG 
GAGATTCGAA TGGCCAAGAA CAACAAGAAC GACATCTACG TGTCCAACAT AACGGAGGAG 
ACGGTTCTGG ATCCAAATCA CCTGCGCCAC CTCATGCACA CGGCCAAGAT GAACCGTGCC 
ACCGCCTCGA CAGCTGGCAA CGAGCGCTCC TCTCGTTCCC ACGCGGTTAC CAAGCTTGAG 
CTCATCGGAC GCCATGCCGA AAAGCAAGAG ATCTCCGTGG GTTCCATAAA CCTGGTGGAT 
TTGGCCGGCT CTGAGTCTCC CAAGACGAGC ACCCGGATGA CCGAGACAAA GAACATCAAT 
CGCTCGCTAT CGGAGCTCAC CAACGTAATC CTGGCGCTGC TGCAGAAGCA GGACCACATC 
CCGTACAGGA ACTCCAAGCT GACGCACCTG CTGATGCCCT CGCTGGGCGG CAACTCGAAA 
ACGCTTATGT TCATCAACGT CTCGCCGTTC CAAGACTGTT TCCAAGAGTC CGTCAAGTCG 
CTGCGCTTCG CGGCCTCCGT AAACTCCTGC AAAATGACCA AGGCCAAGCG GAATCGCTAC 
CTGAACAACT CGGTGGCCAA CAGCAGCACA CAGAGCAACA ACAGCGGCAG TTTCGATAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ncd cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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