Gycalpha99B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gycalpha99B
Entrez Gene ID	43493
Full Name	Guanylyl cyclase alpha-subunit at 99B
Synonyms	CG1912,Dgc1,Dgcalpha1,Dmel\CG1912,GCalpha1,GYCa 99B,Gcalpha1,Gycalpha-99B,Gycalpha63A,SGCalpha,dgc1,dgca1,dgcalpha1,gycalpha,sGC,sGCalpha1,sGCalpha99B
General protein information	Preferred Names Guanylyl cyclase alpha-subunit at 99B Names CG1912 gene product from transcript CG1912-RA CG1912-PA Gycalpha99B-PA guanyl cyclase alpha-subunit at 63A guanylate cyclase 99B guanylyl cyclase alpha-subunit at 99B soluble guanylate cyclase soluble guanylyl cyclase alpha
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99B7-99B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_057740.4	NP_477088.2	guanylyl cyclase alpha-subunit at 99B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GUCY1A2	XP_536590.2
Rattus norvegicus (Norway rat)	Gucy1a2	NP_076446.1
	GUCY1A2	XP_002799832.1
Gallus gallus (chicken)	GUCY1A2	XP_004938891.1
Pan troglodytes (chimpanzee)	GUCY1A2	XP_001136693.1
Bos taurus (cattle)	GUCY1A2	NP_001178270.1
Mus musculus (house mouse)	Gucy1a2	NP_001028494.1
Drosophila melanogaster (fruit fly)	Gycalpha99B	NP_477088.2
Homo sapiens (human)	GUCY1A2	NP_001243353.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000280	XP_310840.5
Xenopus tropicalis (tropical clawed frog)	gucy1a2	NP_001120379.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gycalpha99B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gycalpha99B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06521	NM_057740.4 Latest version!	Drosophila melanogaster guanylyl cyclase alpha-subunit at 99B (Gycalpha99B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gycalpha99B ( NM_057740.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477088.2 Drosophila melanogaster guanylyl cyclase alpha-subunit at 99B (Gycalpha99B), mRNA.

Price & Availability↑

CloneID	OFa06521
Clone ID Related Accession (Same CDS sequence)	NM_057740.4
Accession Version	NM_057740.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase alpha-subunit at 99B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_057740.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTGTC CTTTCTTCAG GCGTGCGGAC TCGCTGACAC GCCAGCCCTC GGTCATCGCC 
GAGCCTGGTG GCCACTGGGC GCTCGAGGAC GAGGAGCTCT CGGACGACGC CCTGACCCTC 
ACCCACCTCC AGATGGCCAT CCAGCTGCTG ACGGCGCCGT CGAACGAGGA TCTCAACACG 
GCTGTAACGT CTTTGGTGGC CAAGTACCGC CAGAACTGGC CCAATATCCA CAAGCTCAAA 
CTGGATCCGC AAACCTTCAA GAGCTGTGCC AACTATGACT ACTTGGCGGA CATCCAGGAG 
CTGCTGCTGA AGATGGACGA GGCCAGCGCC AGCGAGATTC TGGTGCTGCT CGGCGAGGAG 
CTGATCACCT GCTGCTGCAC AGGTATCATT GAAAGAGCCT TCCGCTGCCT GGGCACCGAT 
CTGCAGGAAT TCCTGGGCTC CCTCGATGGC GTTTACGATG TGCTGAAGCT GCAGGAGGAG 
GACGTCACCG ATACCGGATT CGTGTGTGCC GGCGAGGGCG AGCTGATCTT CACCTCGGAG 
CGCCCGGTCA TTGCCTGGTT GCTCCTGGGC TCCCTGAAGG CGCTCACCCG CATGCTCTAC 
AAGGTGGACG TGAACATCAA GATCGAGCCC GTGGAGGGCG ATGCCCGGCG GTATCGCTAC 
CTCTTCTCGC TGGTCAAGGA CAACTCCCAG ACCATGCTGA TGGGTCGTCC CACTTCCGTG 
AGCAAAACCA TCCCGGAAAC GGTGCAGCGG AGCAACTCGA GCAACGCCTC CGACCTGCAA 
ATGAACTCGT CCAGCTTCTG CAAGATGTTT CCCTGGCACT TCATCATGAA CGAGCAGCTG 
GAACTGGTGC AGTTGGGCAG GGGATTCAGC AAATTGTACA AGCCCTACAT GGCGGACTTT 
GGTTGCCAGG CGACCACCTA CTTTGATTTC AAACGACCCA AGGGATTGAC CATGAAATTC 
CGGGACATCG TGAGGCGCAC CTACACGCCT TTCCTGATCG GTTTGAATAA TCCGCCGGGC 
GCTGTGGATT TCCCGGCCAT TGGACTGGAG ATCAAGGGAC AGATGGTGCA CTGTCCCGAG 
TCCAATTCGT TGCTCTTCAT TGGATCCCCC TTCCTGGACG GCCTGGATGG GTTGACCTGC 
AACGGACTCT TCATCTCGGA CATACCGCTG CACGACGCCA CCAGGGAGGT GATCCTGGTG 
GGCGAACAGG CTCGGGCCCA GGATGGACTG CGCCGGCGCA TGGACAAGAT CAAGAACAGC 
ATTGAGGAGG CCAACTCGGC GGTGACCAAG GAGCGGAAGA AGAACGTCAG TCTGCTGCAC 
CTCATCTTTC CCGCGGAGAT CGCCGAGAAG CTTTGGCTGG GCTCCTCCAT TGACGCCAAG 
ACGTATCCCG ATGTTACGAT CCTGTTCAGC GACATCGTTG GCTTCACTAG CATCTGCTCG 
CGGGCCACTC CCTTCATGGT GATCAGCATG CTGGAGGGGC TGTACAAGGA CTTCGACGAG 
TTCTGCGACT TCTTCGACGT GTACAAGGTG GAGACCATCG GGGATGCCTA CTGCGTGGCC 
AGTGGACTCC ACCGAGCCTC CATCTACGAC GCCCACAAGG TTGCCTGGAT GGCCCTGAAG 
ATGATCGACG CCTGCTCGAA GCACATTACC CACGACGGCG AGCAAATTAA AATGAGGATC 
GGCCTGCACA CGGGCACTGT CTTGGCGGGA GTGGTGGGCA GGAAGATGCC CAGGTATTGC 
CTCTTCGGGC ACAGCGTCAC CATCGCCAAC AAGTTCGAGT CGGGCAGCGA GGCGCTGAAG 
ATTAATGTTA GCCCAACCAC CAAGGATTGG CTGACCAAGC ACGAGGGCTT TGAGTTTGAG 
CTGCAGCCGC GGGATCCATC GTTCCTGCCC AAGGAATTCC CCAATCCCGG CGGCACCGAG 
ACCTGCTACT TCCTGGAGAG CTTCCGCAAC CCGGCTTTGG ACAGCGAGCT GCCGCTGGTG 
GAGCACATCA ATGTGTCCAT GAAGACCATA TCCGAGGGCG GCGATGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477088.2
CDS	180..2210
Translation	MACPFFRRADSLTRQPSVIAEPGGHWALEDEELSDDALTLTHLQMAIQLLTAPSNEDLNTAVTSLVAKYRQNWPNIHKLKLDPQTFKSCANYDYLADIQELLLKMDEASASEILVLLGEELITCCCTGIIERAFRCLGTDLQEFLGSLDGVYDVLKLQEEDVTDTGFVCAGEGELIFTSERPVIAWLLLGSLKALTRMLYKVDVNIKIEPVEGDARRYRYLFSLVKDNSQTMLMGRPTSVSKTIPETVQRSNSSNASDLQMNSSSFCKMFPWHFIMNEQLELVQLGRGFSKLYKPYMADFGCQATTYFDFKRPKGLTMKFRDIVRRTYTPFLIGLNNPPGAVDFPAIGLEIKGQMVHCPESNSLLFIGSPFLDGLDGLTCNGLFISDIPLHDATREVILVGEQARAQDGLRRRMDKIKNSIEEANSAVTKERKKNVSLLHLIFPAEIAEKLWLGSSIDAKTYPDVTILFSDIVGFTSICSRATPFMVISMLEGLYKDFDEFCDFFDVYKVETIGDAYCVASGLHRASIYDAHKVAWMALKMIDACSKHITHDGEQIKMRIGLHTGTVLAGVVGRKMPRYCLFGHSVTIANKFESGSEALKINVSPTTKDWLTKHEGFEFELQPRDPSFLPKEFPNPGGTETCYFLESFRNPALDSELPLVEHINVSMKTISEGGDA

Target ORF information:

RefSeq Version	NM_057740.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase alpha-subunit at 99B (Gycalpha99B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057740.4

ORF Insert Sequence:

ATGGCGTGTC CTTTCTTCAG GCGTGCGGAC TCGCTGACAC GCCAGCCCTC GGTCATCGCC 
GAGCCTGGTG GCCACTGGGC GCTCGAGGAC GAGGAGCTCT CGGACGACGC CCTGACCCTC 
ACCCACCTCC AGATGGCCAT CCAGCTGCTG ACGGCGCCGT CGAACGAGGA TCTCAACACG 
GCTGTAACGT CTTTGGTGGC CAAGTACCGC CAGAACTGGC CCAATATCCA CAAGCTCAAA 
CTGGATCCGC AAACCTTCAA GAGCTGTGCC AACTATGACT ACTTGGCGGA CATCCAGGAG 
CTGCTGCTGA AGATGGACGA GGCCAGCGCC AGCGAGATTC TGGTGCTGCT CGGCGAGGAG 
CTGATCACCT GCTGCTGCAC AGGTATCATT GAAAGAGCCT TCCGCTGCCT GGGCACCGAT 
CTGCAGGAAT TCCTGGGCTC CCTCGATGGC GTTTACGATG TGCTGAAGCT GCAGGAGGAG 
GACGTCACCG ATACCGGATT CGTGTGTGCC GGCGAGGGCG AGCTGATCTT CACCTCGGAG 
CGCCCGGTCA TTGCCTGGTT GCTCCTGGGC TCCCTGAAGG CGCTCACCCG CATGCTCTAC 
AAGGTGGACG TGAACATCAA GATCGAGCCC GTGGAGGGCG ATGCCCGGCG GTATCGCTAC 
CTCTTCTCGC TGGTCAAGGA CAACTCCCAG ACCATGCTGA TGGGTCGTCC CACTTCCGTG 
AGCAAAACCA TCCCGGAAAC GGTGCAGCGG AGCAACTCGA GCAACGCCTC CGACCTGCAA 
ATGAACTCGT CCAGCTTCTG CAAGATGTTT CCCTGGCACT TCATCATGAA CGAGCAGCTG 
GAACTGGTGC AGTTGGGCAG GGGATTCAGC AAATTGTACA AGCCCTACAT GGCGGACTTT 
GGTTGCCAGG CGACCACCTA CTTTGATTTC AAACGACCCA AGGGATTGAC CATGAAATTC 
CGGGACATCG TGAGGCGCAC CTACACGCCT TTCCTGATCG GTTTGAATAA TCCGCCGGGC 
GCTGTGGATT TCCCGGCCAT TGGACTGGAG ATCAAGGGAC AGATGGTGCA CTGTCCCGAG 
TCCAATTCGT TGCTCTTCAT TGGATCCCCC TTCCTGGACG GCCTGGATGG GTTGACCTGC 
AACGGACTCT TCATCTCGGA CATACCGCTG CACGACGCCA CCAGGGAGGT GATCCTGGTG 
GGCGAACAGG CTCGGGCCCA GGATGGACTG CGCCGGCGCA TGGACAAGAT CAAGAACAGC 
ATTGAGGAGG CCAACTCGGC GGTGACCAAG GAGCGGAAGA AGAACGTCAG TCTGCTGCAC 
CTCATCTTTC CCGCGGAGAT CGCCGAGAAG CTTTGGCTGG GCTCCTCCAT TGACGCCAAG 
ACGTATCCCG ATGTTACGAT CCTGTTCAGC GACATCGTTG GCTTCACTAG CATCTGCTCG 
CGGGCCACTC CCTTCATGGT GATCAGCATG CTGGAGGGGC TGTACAAGGA CTTCGACGAG 
TTCTGCGACT TCTTCGACGT GTACAAGGTG GAGACCATCG GGGATGCCTA CTGCGTGGCC 
AGTGGACTCC ACCGAGCCTC CATCTACGAC GCCCACAAGG TTGCCTGGAT GGCCCTGAAG 
ATGATCGACG CCTGCTCGAA GCACATTACC CACGACGGCG AGCAAATTAA AATGAGGATC 
GGCCTGCACA CGGGCACTGT CTTGGCGGGA GTGGTGGGCA GGAAGATGCC CAGGTATTGC 
CTCTTCGGGC ACAGCGTCAC CATCGCCAAC AAGTTCGAGT CGGGCAGCGA GGCGCTGAAG 
ATTAATGTTA GCCCAACCAC CAAGGATTGG CTGACCAAGC ACGAGGGCTT TGAGTTTGAG 
CTGCAGCCGC GGGATCCATC GTTCCTGCCC AAGGAATTCC CCAATCCCGG CGGCACCGAG 
ACCTGCTACT TCCTGGAGAG CTTCCGCAAC CCGGCTTTGG ACAGCGAGCT GCCGCTGGTG 
GAGCACATCA ATGTGTCCAT GAAGACCATA TCCGAGGGCG GCGATGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gycalpha99B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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