Bub3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bub3
Entrez Gene ID	43490
Full Name	CG7581 gene product from transcript CG7581-RB
Synonyms	BUB3,CG7581,Dmbub3,Dmel\CG7581,bub3,dBUB3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99B7-99B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300658.1	NP_001287587.1	Bub3, isoform B
NM_058033.4	NP_477381.1	Bub3, isoform A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Bub3	NP_001041371.2
Arabidopsis thaliana (thale cress)	BUB3.1	NP_566644.1
Pan troglodytes (chimpanzee)	BUB3	XP_001161211.1
	BUB3	XP_001105657.1
Mus musculus (house mouse)	Bub3	NP_033904.2
Gallus gallus (chicken)	BUB3	NP_001006506.1
Oryza sativa (rice)	Os03g0448600	NP_001050471.1
Xenopus tropicalis (tropical clawed frog)	bub3	NP_001007498.1
Arabidopsis thaliana (thale cress)	BUB3.2	NP_175413.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010544	XP_314512.3
Homo sapiens (human)	BUB3	NP_004716.1
Bos taurus (cattle)	BUB3	NP_001069645.1
Danio rerio (zebrafish)	bub3	NP_991272.1
Drosophila melanogaster (fruit fly)	Bub3	NP_477381.1
Schizosaccharomyces pombe (fission yeast)	bub3	NP_593798.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Bub3-BubR1 complex on broken DNA inhibits the APC/C locally via the sequestration of Cdc20.
Title: Bub3-BubR1-dependent sequestration of Cdc20Fizzy at DNA breaks facilitates the correct segregation of broken chromosomes.

Bub3 has a nonkinetochore-dependent function that is consistent with its role as a tumor suppressor.
Title: A tumor suppressor role of the Bub3 spindle checkpoint protein after apoptosis inhibition.

Bub3 is required for the mitotic checkpoint and for normal accumulation of cyclins during G2 and early stages of mitosis.
Title: The Drosophila Bub3 protein is required for the mitotic checkpoint and for normal accumulation of cyclins during G2 and early stages of mitosis.

The following Bub3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bub3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06518	NM_001300658.1 Latest version!	Drosophila melanogaster Bub3, transcript variant B (Bub3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06518	NM_058033.4 Latest version!	Drosophila melanogaster Bub3, transcript variant A (Bub3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bub3 ( NM_001300658.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287587.1 Drosophila melanogaster Bub3, transcript variant B (Bub3), mRNA.

Price & Availability↑

CloneID	OFa06518
Clone ID Related Accession (Same CDS sequence)	NM_001300658.1 , NM_058033.4
Accession Version	NM_001300658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Bub3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCCCC CAGAGTTCAA GCTTAACAAT CCGCCGGAGG ACCTGATCTC CGCTGTGAAA 
TTCGGCCCCA AATCGAATCA ATACATGGCG GCGTCTTCTT GGGACGGAAC ACTTAGATTC 
TATGATGTGC CGGCAAACCA GCTGCGTCAG AAATTCGTGC AGGATGCCCC TCTTCTCGAC 
TGTGCCTTCA TGGACATAGT GCACGTGGTC AGTGGCTCCC TGGACAACCA GCTGCGTCTC 
TTTGATGTCA ACACCCAGGC GGAAAGCATA ATTGGTGCCC ACGAGGAGCC CATCAGATGC 
GTGGAGCACG CCGAGTATGT CAATGGCATC CTCACGGGTA GCTGGGACAA TACCGTGAAG 
CTGTGGGACA TGCGGGAGAA GCGCTGCGTT GGAACCTTCG AACAGAACAA CGGAAAGGTG 
TACTCCATGT CCGTGATCGA CGAAAAGATC GTGGTGGCCA CCTCCGACCG CAAGGTGCTC 
ATCTGGGACC TTCGCAAGAT GGACAGCTAC ATCATGAAGC GGGAGTCCTC GCTCAAGTAC 
CAGACCCGCT GCATTCGTCT ATTCCCCAAC AAAGAAGGCT ACGTGATGTC CTCCATCGAG 
GGACGGGTGG CAGTGGAGTA CCTGGATCAC GATCCTGAGG TGCAGCGCCG CAAGTTCGCT 
TTCAAGTGCC ACCGGAATAG GGAGCAGAAT ATCGAACAGA TCTACCCCGT TAATGCCTTG 
AGTTTCCACA ATGTCTATCA GACGTTCGCC ACTGGCGGAT CCGATGGCAT TGTGAACATT 
TGGGATGGCT TCAACAAGAA GCGCCTTTGT CAGTTCCACG AATACGATAC CTCCATATCC 
ACGCTCAACT TCAGCTCCGA TGGCAGCGCC CTGGCCATCG GCTGCTCCTA CTTGGACCAA 
CTGCCAGAGA CGCCGGCTAC AGTGCCACAT CCGGCTATCT ATATACGCTA TCCCACGGAC 
CAGGAAACTA AACAGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287587.1
CDS	114..1094
Translation	MRPPEFKLNNPPEDLISAVKFGPKSNQYMAASSWDGTLRFYDVPANQLRQKFVQDAPLLDCAFMDIVHVVSGSLDNQLRLFDVNTQAESIIGAHEEPIRCVEHAEYVNGILTGSWDNTVKLWDMREKRCVGTFEQNNGKVYSMSVIDEKIVVATSDRKVLIWDLRKMDSYIMKRESSLKYQTRCIRLFPNKEGYVMSSIEGRVAVEYLDHDPEVQRRKFAFKCHRNREQNIEQIYPVNALSFHNVYQTFATGGSDGIVNIWDGFNKKRLCQFHEYDTSISTLNFSSDGSALAIGCSYLDQLPETPATVPHPAIYIRYPTDQETKQK

Target ORF information:

RefSeq Version	NM_001300658.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Bub3, transcript variant B (Bub3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300658.1

ORF Insert Sequence:

ATGCGTCCCC CAGAGTTCAA GCTTAACAAT CCGCCGGAGG ACCTGATCTC CGCTGTGAAA 
TTCGGCCCCA AATCGAATCA ATACATGGCG GCGTCTTCTT GGGACGGAAC ACTTAGATTC 
TATGATGTGC CGGCAAACCA GCTGCGTCAG AAATTCGTGC AGGATGCCCC TCTTCTCGAC 
TGTGCCTTCA TGGACATAGT GCACGTGGTC AGTGGCTCCC TGGACAACCA GCTGCGTCTC 
TTTGATGTCA ACACCCAGGC GGAAAGCATA ATTGGTGCCC ACGAGGAGCC CATCAGATGC 
GTGGAGCACG CCGAGTATGT CAATGGCATC CTCACGGGTA GCTGGGACAA TACCGTGAAG 
CTGTGGGACA TGCGGGAGAA GCGCTGCGTT GGAACCTTCG AACAGAACAA CGGAAAGGTG 
TACTCCATGT CCGTGATCGA CGAAAAGATC GTGGTGGCCA CCTCCGACCG CAAGGTGCTC 
ATCTGGGACC TTCGCAAGAT GGACAGCTAC ATCATGAAGC GGGAGTCCTC GCTCAAGTAC 
CAGACCCGCT GCATTCGTCT ATTCCCCAAC AAAGAAGGCT ACGTGATGTC CTCCATCGAG 
GGACGGGTGG CAGTGGAGTA CCTGGATCAC GATCCTGAGG TGCAGCGCCG CAAGTTCGCT 
TTCAAGTGCC ACCGGAATAG GGAGCAGAAT ATCGAACAGA TCTACCCCGT TAATGCCTTG 
AGTTTCCACA ATGTCTATCA GACGTTCGCC ACTGGCGGAT CCGATGGCAT TGTGAACATT 
TGGGATGGCT TCAACAAGAA GCGCCTTTGT CAGTTCCACG AATACGATAC CTCCATATCC 
ACGCTCAACT TCAGCTCCGA TGGCAGCGCC CTGGCCATCG GCTGCTCCTA CTTGGACCAA 
CTGCCAGAGA CGCCGGCTAC AGTGCCACAT CCGGCTATCT ATATACGCTA TCCCACGGAC 
CAGGAAACTA AACAGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bub3 ( NM_058033.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477381.1 Drosophila melanogaster Bub3, transcript variant A (Bub3), mRNA.

Price & Availability↑

CloneID	OFa06518
Clone ID Related Accession (Same CDS sequence)	NM_001300658.1 , NM_058033.4
Accession Version	NM_058033.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Bub3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_058033.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCCCC CAGAGTTCAA GCTTAACAAT CCGCCGGAGG ACCTGATCTC CGCTGTGAAA 
TTCGGCCCCA AATCGAATCA ATACATGGCG GCGTCTTCTT GGGACGGAAC ACTTAGATTC 
TATGATGTGC CGGCAAACCA GCTGCGTCAG AAATTCGTGC AGGATGCCCC TCTTCTCGAC 
TGTGCCTTCA TGGACATAGT GCACGTGGTC AGTGGCTCCC TGGACAACCA GCTGCGTCTC 
TTTGATGTCA ACACCCAGGC GGAAAGCATA ATTGGTGCCC ACGAGGAGCC CATCAGATGC 
GTGGAGCACG CCGAGTATGT CAATGGCATC CTCACGGGTA GCTGGGACAA TACCGTGAAG 
CTGTGGGACA TGCGGGAGAA GCGCTGCGTT GGAACCTTCG AACAGAACAA CGGAAAGGTG 
TACTCCATGT CCGTGATCGA CGAAAAGATC GTGGTGGCCA CCTCCGACCG CAAGGTGCTC 
ATCTGGGACC TTCGCAAGAT GGACAGCTAC ATCATGAAGC GGGAGTCCTC GCTCAAGTAC 
CAGACCCGCT GCATTCGTCT ATTCCCCAAC AAAGAAGGCT ACGTGATGTC CTCCATCGAG 
GGACGGGTGG CAGTGGAGTA CCTGGATCAC GATCCTGAGG TGCAGCGCCG CAAGTTCGCT 
TTCAAGTGCC ACCGGAATAG GGAGCAGAAT ATCGAACAGA TCTACCCCGT TAATGCCTTG 
AGTTTCCACA ATGTCTATCA GACGTTCGCC ACTGGCGGAT CCGATGGCAT TGTGAACATT 
TGGGATGGCT TCAACAAGAA GCGCCTTTGT CAGTTCCACG AATACGATAC CTCCATATCC 
ACGCTCAACT TCAGCTCCGA TGGCAGCGCC CTGGCCATCG GCTGCTCCTA CTTGGACCAA 
CTGCCAGAGA CGCCGGCTAC AGTGCCACAT CCGGCTATCT ATATACGCTA TCCCACGGAC 
CAGGAAACTA AACAGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477381.1
CDS	114..1094
Translation	MRPPEFKLNNPPEDLISAVKFGPKSNQYMAASSWDGTLRFYDVPANQLRQKFVQDAPLLDCAFMDIVHVVSGSLDNQLRLFDVNTQAESIIGAHEEPIRCVEHAEYVNGILTGSWDNTVKLWDMREKRCVGTFEQNNGKVYSMSVIDEKIVVATSDRKVLIWDLRKMDSYIMKRESSLKYQTRCIRLFPNKEGYVMSSIEGRVAVEYLDHDPEVQRRKFAFKCHRNREQNIEQIYPVNALSFHNVYQTFATGGSDGIVNIWDGFNKKRLCQFHEYDTSISTLNFSSDGSALAIGCSYLDQLPETPATVPHPAIYIRYPTDQETKQK

Target ORF information:

RefSeq Version	NM_058033.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Bub3, transcript variant A (Bub3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058033.4

ORF Insert Sequence:

ATGCGTCCCC CAGAGTTCAA GCTTAACAAT CCGCCGGAGG ACCTGATCTC CGCTGTGAAA 
TTCGGCCCCA AATCGAATCA ATACATGGCG GCGTCTTCTT GGGACGGAAC ACTTAGATTC 
TATGATGTGC CGGCAAACCA GCTGCGTCAG AAATTCGTGC AGGATGCCCC TCTTCTCGAC 
TGTGCCTTCA TGGACATAGT GCACGTGGTC AGTGGCTCCC TGGACAACCA GCTGCGTCTC 
TTTGATGTCA ACACCCAGGC GGAAAGCATA ATTGGTGCCC ACGAGGAGCC CATCAGATGC 
GTGGAGCACG CCGAGTATGT CAATGGCATC CTCACGGGTA GCTGGGACAA TACCGTGAAG 
CTGTGGGACA TGCGGGAGAA GCGCTGCGTT GGAACCTTCG AACAGAACAA CGGAAAGGTG 
TACTCCATGT CCGTGATCGA CGAAAAGATC GTGGTGGCCA CCTCCGACCG CAAGGTGCTC 
ATCTGGGACC TTCGCAAGAT GGACAGCTAC ATCATGAAGC GGGAGTCCTC GCTCAAGTAC 
CAGACCCGCT GCATTCGTCT ATTCCCCAAC AAAGAAGGCT ACGTGATGTC CTCCATCGAG 
GGACGGGTGG CAGTGGAGTA CCTGGATCAC GATCCTGAGG TGCAGCGCCG CAAGTTCGCT 
TTCAAGTGCC ACCGGAATAG GGAGCAGAAT ATCGAACAGA TCTACCCCGT TAATGCCTTG 
AGTTTCCACA ATGTCTATCA GACGTTCGCC ACTGGCGGAT CCGATGGCAT TGTGAACATT 
TGGGATGGCT TCAACAAGAA GCGCCTTTGT CAGTTCCACG AATACGATAC CTCCATATCC 
ACGCTCAACT TCAGCTCCGA TGGCAGCGCC CTGGCCATCG GCTGCTCCTA CTTGGACCAA 
CTGCCAGAGA CGCCGGCTAC AGTGCCACAT CCGGCTATCT ATATACGCTA TCCCACGGAC 
CAGGAAACTA AACAGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Bub3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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