ppk20 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppk20
Entrez Gene ID	43486
Full Name	pickpocket 20
Synonyms	CG7577,Dmel\CG7577,PPK20
General protein information	Preferred Names pickpocket 20 Names CG7577 gene product from transcript CG7577-RB CG7577-PA CG7577-PB CG7577-PC ppk20-PA ppk20-PB ppk20-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99B6-99B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276145.1	NP_001263074.1	pickpocket 20, isoform B
NM_001276146.1	NP_001263075.1	pickpocket 20, isoform C
NM_143448.2	NP_651705.2	pickpocket 20, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	ppk14	NP_609017.1
Drosophila melanogaster (fruit fly)	ppk19	NP_651708.2
Drosophila melanogaster (fruit fly)	ppk7	NP_609016.2
Drosophila melanogaster (fruit fly)	CG18110	NP_001263076.1
Drosophila melanogaster (fruit fly)	ppk21	NP_001263073.1
Drosophila melanogaster (fruit fly)	ppk20	NP_001263075.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ppk20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppk20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06514	NM_143448.2 Latest version!	Drosophila melanogaster pickpocket 20 (ppk20), transcript variant A, partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa08955	NM_001276145.1 Latest version!	Drosophila melanogaster pickpocket 20 (ppk20), transcript variant B, partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa08956	NM_001276146.1 Latest version!	Drosophila melanogaster pickpocket 20 (ppk20), transcript variant C, partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppk20 ( NM_143448.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651705.2 Drosophila melanogaster pickpocket 20 (ppk20), transcript variant A, partial mRNA.

Price & Availability↑

CloneID	OFa06514
Clone ID Related Accession (Same CDS sequence)	NM_143448.2
Accession Version	NM_143448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pickpocket 20, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Apr 11, 2005 this sequence version replaced NM_143448.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCCAAGG GAGATAATTC GGTCAAACCG ACAGAGGCAG CCGGTTTTGG TGACAACGAG 
AGGTGCCTCG CGGATTTGTT AAAGGTCCAC TTTCGGAGCT ACTGCGAGAA GTCCACCATT 
CACTGCGTTC GCTATCTGTA CGATTCCCAC CTGCATAACT TGGAGAGGTT TGTGGTTTAT 
CCTCTATTCA TATCTACATT CAATTTTAAA TTGTATATAA TCATTGTATT TAGAATCATC 
TGGTCGATGC TGCTCATCAT TAGCATCGGG CTGTCCTTCT TTTTCTACCT CCTGCTCTCC 
GAGCGATTCG TTTCCCAGAA GCTGCAGACG GTGGTCCACG ATCCGCAGTT CCCCGTGTTT 
CTGGTGCCAT TTCCGGCCGT GGGAATCTGC ACGGACAACC GCATCAACTG GAACAAGCTG 
GAGGCGGCCA AGGAGCAGTT CCTGCCGACC AATGCCAGCG TGGAACTGGT CGAAAGTTTC 
ACCGTCCTTG TTTCTCGGAT GGAAACTTTG CGCTTTGGCA GTTACCTTTC AAGCTTGGCC 
GAGCTGGAGG ACGATAACCT GGAGGCGGTG GGCTTTGTGA ACCTAACTGA GCTGGCCATA 
TTTATGACTC TTCAATGCTC GGATATCATG GTACCGAAAT CCTGCCTGTG GCGCAGTTCC 
TCCTTCAATT GCTGCGAATA CTTCGTGTTG GAGAAGACGG AGTTTGGATT TTGTTTGGTT 
TTCAACTCGG AGGTTTCGCC ACGCTCCAAG GCAATTAAGC AGAAGGAGGG TAATAACTTC 
TATCCGAGGC ACAATGCCAA AGCAGGCCAG AGTACGGGCT TGAATTTTGA TCTAATCTTA 
AACGAGAGCT TCAGGCGACC CGATTCGCAG GCGAATAATA ATGTCTACGT AAGTATCTGC 
CAGGCCCCCG ACCAACTGAA CAATGTCGTA TATTCAATAA CCCAAAACAC GGAGACCTAC 
GTGACTGTCC GTCCGGGTCT CACTTGGACG GATAACACCA CGCGCAGTAT CCCGCCGGAG 
CGCAGGAACT GCCTCTTCGC GGACGAGCAG GGCGAACTGG ACGCCAACGA TTCGGCCAAG 
AACTTTGGAA AGCCCTTCCA GCTCTCCAAC TGTCTCAACC GCTGCCATGA GTCCTACCTA 
ATTCAGCTCT GCAACTGCTC CTTGCCCATT TTCTTCCTGT ACAACCACAG AGTTCCGGAT 
TGCAACGCAG TCAGCCTGCG CTGCCTTGCG CGTCACAATG ACATCTTCAG CTACGACAAG 
CGAAGGGACG AGGATGCCCT TTTCAGTGCC ACCAAGCTGG GAATGACCTG TTCCTGCTTG 
GTGGACTGCT ACCTGCTCGA CTATTATACT TCCACCACCA CACTGCCTTT GTCAGCCCAT 
AAACTACCCA AGGATCCGCA TCAGAAACTC TTCAGGGTGG ATGTGCACTA CCAAGTGGAG 
ACCACACCTT TGTATCGCAC AAGCCTGGAG TTTACCATTA TCGATCTAAT TGCCAATCTG 
GGTGGAATCT TTGGACTTTG CTTGGGTGCT TCTATGGTCA GTGCCTTCGA GTTGATCTAC 
TACCTTACTG TGGGACTTGC TATGCATCTA TATGACCACC AGTACTATGG GGTCTTATTT 
AAGCACCTGA AGGCTAAGTG GGTCAATTTA AAGGGCTACC TAAGGAACGA AGTTGGCCAT 
CTGGCTGAGA ATCCCGCTGC CCACAAGGAT ACCAATGACA GGAAATTGAG GCATCCTTTT 
AACTACAGAA AGAATGTGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651705.2
CDS	1..1764
Translation	MAKGDNSVKPTEAAGFGDNERCLADLLKVHFRSYCEKSTIHCVRYLYDSHLHNLERFVVYPLFISTFNFKLYIIIVFRIIWSMLLIISIGLSFFFYLLLSERFVSQKLQTVVHDPQFPVFLVPFPAVGICTDNRINWNKLEAAKEQFLPTNASVELVESFTVLVSRMETLRFGSYLSSLAELEDDNLEAVGFVNLTELAIFMTLQCSDIMVPKSCLWRSSSFNCCEYFVLEKTEFGFCLVFNSEVSPRSKAIKQKEGNNFYPRHNAKAGQSTGLNFDLILNESFRRPDSQANNNVYVSICQAPDQLNNVVYSITQNTETYVTVRPGLTWTDNTTRSIPPERRNCLFADEQGELDANDSAKNFGKPFQLSNCLNRCHESYLIQLCNCSLPIFFLYNHRVPDCNAVSLRCLARHNDIFSYDKRRDEDALFSATKLGMTCSCLVDCYLLDYYTSTTTLPLSAHKLPKDPHQKLFRVDVHYQVETTPLYRTSLEFTIIDLIANLGGIFGLCLGASMVSAFELIYYLTVGLAMHLYDHQYYGVLFKHLKAKWVNLKGYLRNEVGHLAENPAAHKDTNDRKLRHPFNYRKNVW

Target ORF information:

RefSeq Version	NM_143448.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pickpocket 20 (ppk20), transcript variant A, partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143448.2

ORF Insert Sequence:

ATGGCCAAGG GAGATAATTC GGTCAAACCG ACAGAGGCAG CCGGTTTTGG TGACAACGAG 
AGGTGCCTCG CGGATTTGTT AAAGGTCCAC TTTCGGAGCT ACTGCGAGAA GTCCACCATT 
CACTGCGTTC GCTATCTGTA CGATTCCCAC CTGCATAACT TGGAGAGGTT TGTGGTTTAT 
CCTCTATTCA TATCTACATT CAATTTTAAA TTGTATATAA TCATTGTATT TAGAATCATC 
TGGTCGATGC TGCTCATCAT TAGCATCGGG CTGTCCTTCT TTTTCTACCT CCTGCTCTCC 
GAGCGATTCG TTTCCCAGAA GCTGCAGACG GTGGTCCACG ATCCGCAGTT CCCCGTGTTT 
CTGGTGCCAT TTCCGGCCGT GGGAATCTGC ACGGACAACC GCATCAACTG GAACAAGCTG 
GAGGCGGCCA AGGAGCAGTT CCTGCCGACC AATGCCAGCG TGGAACTGGT CGAAAGTTTC 
ACCGTCCTTG TTTCTCGGAT GGAAACTTTG CGCTTTGGCA GTTACCTTTC AAGCTTGGCC 
GAGCTGGAGG ACGATAACCT GGAGGCGGTG GGCTTTGTGA ACCTAACTGA GCTGGCCATA 
TTTATGACTC TTCAATGCTC GGATATCATG GTACCGAAAT CCTGCCTGTG GCGCAGTTCC 
TCCTTCAATT GCTGCGAATA CTTCGTGTTG GAGAAGACGG AGTTTGGATT TTGTTTGGTT 
TTCAACTCGG AGGTTTCGCC ACGCTCCAAG GCAATTAAGC AGAAGGAGGG TAATAACTTC 
TATCCGAGGC ACAATGCCAA AGCAGGCCAG AGTACGGGCT TGAATTTTGA TCTAATCTTA 
AACGAGAGCT TCAGGCGACC CGATTCGCAG GCGAATAATA ATGTCTACGT AAGTATCTGC 
CAGGCCCCCG ACCAACTGAA CAATGTCGTA TATTCAATAA CCCAAAACAC GGAGACCTAC 
GTGACTGTCC GTCCGGGTCT CACTTGGACG GATAACACCA CGCGCAGTAT CCCGCCGGAG 
CGCAGGAACT GCCTCTTCGC GGACGAGCAG GGCGAACTGG ACGCCAACGA TTCGGCCAAG 
AACTTTGGAA AGCCCTTCCA GCTCTCCAAC TGTCTCAACC GCTGCCATGA GTCCTACCTA 
ATTCAGCTCT GCAACTGCTC CTTGCCCATT TTCTTCCTGT ACAACCACAG AGTTCCGGAT 
TGCAACGCAG TCAGCCTGCG CTGCCTTGCG CGTCACAATG ACATCTTCAG CTACGACAAG 
CGAAGGGACG AGGATGCCCT TTTCAGTGCC ACCAAGCTGG GAATGACCTG TTCCTGCTTG 
GTGGACTGCT ACCTGCTCGA CTATTATACT TCCACCACCA CACTGCCTTT GTCAGCCCAT 
AAACTACCCA AGGATCCGCA TCAGAAACTC TTCAGGGTGG ATGTGCACTA CCAAGTGGAG 
ACCACACCTT TGTATCGCAC AAGCCTGGAG TTTACCATTA TCGATCTAAT TGCCAATCTG 
GGTGGAATCT TTGGACTTTG CTTGGGTGCT TCTATGGTCA GTGCCTTCGA GTTGATCTAC 
TACCTTACTG TGGGACTTGC TATGCATCTA TATGACCACC AGTACTATGG GGTCTTATTT 
AAGCACCTGA AGGCTAAGTG GGTCAATTTA AAGGGCTACC TAAGGAACGA AGTTGGCCAT 
CTGGCTGAGA ATCCCGCTGC CCACAAGGAT ACCAATGACA GGAAATTGAG GCATCCTTTT 
AACTACAGAA AGAATGTGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppk20 ( NM_001276145.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263074.1 Drosophila melanogaster pickpocket 20 (ppk20), transcript variant B, partial mRNA.

Price & Availability↑

CloneID	OFa08955
Clone ID Related Accession (Same CDS sequence)	NM_001276145.1
Accession Version	NM_001276145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pickpocket 20, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCCAAGG GAGATAATTC GGTCAAACCG ACAGAGGCAG CCGGTTTTGG TGACAACGAG 
AGGTGCCTCG CGGATTTGTT AAAGGTCCAC TTTCGGAGCT ACTGCGAGAA GTCCACCATT 
CACTGCGTTC GCTATCTGTA CGATTCCCAC CTGCATAACT TGGAGAGAAT CATCTGGTCG 
ATGCTGCTCA TCATTAGCAT CGGGCTGTCC TTCTTTTTCT ACCTCCTGCT CTCCGAGCGA 
TTCGTTTCCC AGAAGCTGCA GACGGTGGTC CACGATCCGC AGTTCCCCGT GTTTCTGGTG 
CCATTTCCGG CCGTGGGAAT CTGCACGGAC AACCGCATCA ACTGGAACAA GCTGGAGGCG 
GCCAAGGAGC AGTTCCTGCC GACCAATGCC AGCGTGGAAC TGGTCGAAAG TTTCACCGTC 
CTTGTTTCTC GGATGGAAAC TTTGCGCTTT GGCAGTTACC TTTCAAGCTT GGCCGAGCTG 
GAGGACGATA ACCTGGAGGC GGTGGGCTTT GTGAACCTAA CTGAGCTGGC CATATTTATG 
ACTCTTCAAT GCTCGGATAT CATGGTACCG AAATCCTGCC TGTGGCGCAG TTCCTCCTTC 
AATTGCTGCG AATACTTCGT GTTGGAGAAG ACGGAGTTTG GATTTTGTTT GGTTTTCAAC 
TCGGAGGTTT CGCCACGCTC CAAGGCAATT AAGCAGAAGG AGGGTAATAA CTTCTATCCG 
AGGCACAATG CCAAAGCAGG CCAGAGTACG GGCTTGAATT TTGATCTAAT CTTAAACGAG 
AGCTTCAGGC GACCCGATTC GCAGGCGAAT AATAATGTCT ACGTTATGAT TAAGAAGCCC 
GACCAACTGA ACAATGTCGT ATATTCAATA ACCCAAAACA CGGAGACCTA CGTGACTGTC 
CGTCCGGGTC TCACTTGGAC GGATAACACC ACGCGCAGTA TCCCGCCGGA GCGCAGGAAC 
TGCCTCTTCG CGGACGAGCA GGGCGAACTG GACGCCAACG ATTCGGCCAA GAACTTTGGA 
AAGCCCTTCC AGCTCTCCAA CTGTCTCAAC CGCTGCCATG AGTCCTACCT AATTCAGCTC 
TGCAACTGCT CCTTGCCCAT TTTCTTCCTG TACAACCACA GAGTTCCGGA TTGCAACGCA 
GTCAGCCTGC GCTGCCTTGC GCGTCACAAT GACATCTTCA GCTACGACAA GCGAAGGGAC 
GAGGATGCCC TTTTCAGTGC CACCAAGCTG GGAATGACCT GTTCCTGCTT GGTGGACTGC 
TACCTGCTCG ACTATTATAC TTCCACCACC ACACTGCCTT TGTCAGCCCA TAAACTACCC 
AAGGATCCGC ATCAGAAACT CTTCAGGGTG GATGTGCACT ACCAAGTGGA GACCACACCT 
TTGTATCGCA CAAGCCTGGA GTTTACCATT ATCGATCTAA TTGCCAATCT GGGTGGAATC 
TTTGGACTTT GCTTGGGTGC TTCTATGGTC AGTGCCTTCG AGTTGATCTA CTACCTTACT 
GTGGGACTTG CTATGCATCT ATATGACCAC CAGTACTATG GGGTCTTATT TAAGCACCTG 
AAGGCTAAGT GGGTCAATTT AAAGGGCTAC CTAAGGAACG AAGTTGGCCA TCTGGCTGAG 
AATCCCGCTG CCCACAAGGA TACCAATGAC AGGAAATTGA GGCATCCTTT TAACTACAGA 
AAGAATGTGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263074.1
CDS	1..1695
Translation	MAKGDNSVKPTEAAGFGDNERCLADLLKVHFRSYCEKSTIHCVRYLYDSHLHNLERIIWSMLLIISIGLSFFFYLLLSERFVSQKLQTVVHDPQFPVFLVPFPAVGICTDNRINWNKLEAAKEQFLPTNASVELVESFTVLVSRMETLRFGSYLSSLAELEDDNLEAVGFVNLTELAIFMTLQCSDIMVPKSCLWRSSSFNCCEYFVLEKTEFGFCLVFNSEVSPRSKAIKQKEGNNFYPRHNAKAGQSTGLNFDLILNESFRRPDSQANNNVYVMIKKPDQLNNVVYSITQNTETYVTVRPGLTWTDNTTRSIPPERRNCLFADEQGELDANDSAKNFGKPFQLSNCLNRCHESYLIQLCNCSLPIFFLYNHRVPDCNAVSLRCLARHNDIFSYDKRRDEDALFSATKLGMTCSCLVDCYLLDYYTSTTTLPLSAHKLPKDPHQKLFRVDVHYQVETTPLYRTSLEFTIIDLIANLGGIFGLCLGASMVSAFELIYYLTVGLAMHLYDHQYYGVLFKHLKAKWVNLKGYLRNEVGHLAENPAAHKDTNDRKLRHPFNYRKNVW

Target ORF information:

RefSeq Version	NM_001276145.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pickpocket 20 (ppk20), transcript variant B, partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276145.1

ORF Insert Sequence:

ATGGCCAAGG GAGATAATTC GGTCAAACCG ACAGAGGCAG CCGGTTTTGG TGACAACGAG 
AGGTGCCTCG CGGATTTGTT AAAGGTCCAC TTTCGGAGCT ACTGCGAGAA GTCCACCATT 
CACTGCGTTC GCTATCTGTA CGATTCCCAC CTGCATAACT TGGAGAGAAT CATCTGGTCG 
ATGCTGCTCA TCATTAGCAT CGGGCTGTCC TTCTTTTTCT ACCTCCTGCT CTCCGAGCGA 
TTCGTTTCCC AGAAGCTGCA GACGGTGGTC CACGATCCGC AGTTCCCCGT GTTTCTGGTG 
CCATTTCCGG CCGTGGGAAT CTGCACGGAC AACCGCATCA ACTGGAACAA GCTGGAGGCG 
GCCAAGGAGC AGTTCCTGCC GACCAATGCC AGCGTGGAAC TGGTCGAAAG TTTCACCGTC 
CTTGTTTCTC GGATGGAAAC TTTGCGCTTT GGCAGTTACC TTTCAAGCTT GGCCGAGCTG 
GAGGACGATA ACCTGGAGGC GGTGGGCTTT GTGAACCTAA CTGAGCTGGC CATATTTATG 
ACTCTTCAAT GCTCGGATAT CATGGTACCG AAATCCTGCC TGTGGCGCAG TTCCTCCTTC 
AATTGCTGCG AATACTTCGT GTTGGAGAAG ACGGAGTTTG GATTTTGTTT GGTTTTCAAC 
TCGGAGGTTT CGCCACGCTC CAAGGCAATT AAGCAGAAGG AGGGTAATAA CTTCTATCCG 
AGGCACAATG CCAAAGCAGG CCAGAGTACG GGCTTGAATT TTGATCTAAT CTTAAACGAG 
AGCTTCAGGC GACCCGATTC GCAGGCGAAT AATAATGTCT ACGTTATGAT TAAGAAGCCC 
GACCAACTGA ACAATGTCGT ATATTCAATA ACCCAAAACA CGGAGACCTA CGTGACTGTC 
CGTCCGGGTC TCACTTGGAC GGATAACACC ACGCGCAGTA TCCCGCCGGA GCGCAGGAAC 
TGCCTCTTCG CGGACGAGCA GGGCGAACTG GACGCCAACG ATTCGGCCAA GAACTTTGGA 
AAGCCCTTCC AGCTCTCCAA CTGTCTCAAC CGCTGCCATG AGTCCTACCT AATTCAGCTC 
TGCAACTGCT CCTTGCCCAT TTTCTTCCTG TACAACCACA GAGTTCCGGA TTGCAACGCA 
GTCAGCCTGC GCTGCCTTGC GCGTCACAAT GACATCTTCA GCTACGACAA GCGAAGGGAC 
GAGGATGCCC TTTTCAGTGC CACCAAGCTG GGAATGACCT GTTCCTGCTT GGTGGACTGC 
TACCTGCTCG ACTATTATAC TTCCACCACC ACACTGCCTT TGTCAGCCCA TAAACTACCC 
AAGGATCCGC ATCAGAAACT CTTCAGGGTG GATGTGCACT ACCAAGTGGA GACCACACCT 
TTGTATCGCA CAAGCCTGGA GTTTACCATT ATCGATCTAA TTGCCAATCT GGGTGGAATC 
TTTGGACTTT GCTTGGGTGC TTCTATGGTC AGTGCCTTCG AGTTGATCTA CTACCTTACT 
GTGGGACTTG CTATGCATCT ATATGACCAC CAGTACTATG GGGTCTTATT TAAGCACCTG 
AAGGCTAAGT GGGTCAATTT AAAGGGCTAC CTAAGGAACG AAGTTGGCCA TCTGGCTGAG 
AATCCCGCTG CCCACAAGGA TACCAATGAC AGGAAATTGA GGCATCCTTT TAACTACAGA 
AAGAATGTGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppk20 ( NM_001276146.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263075.1 Drosophila melanogaster pickpocket 20 (ppk20), transcript variant C, partial mRNA.

Price & Availability↑

CloneID	OFa08956
Clone ID Related Accession (Same CDS sequence)	NM_001276146.1
Accession Version	NM_001276146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pickpocket 20, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCCAAGG GAGATAATTC GGTCAAACCG ACAGAGGCAG CCGGTTTTGG TGACAACGAG 
AGGTGCCTCG CGGATTTGTT AAAGGTCCAC TTTCGGAGCT ACTGCGAGAA GTCCACCATT 
CACTGCGTTC GCTATCTGTA CGATTCCCAC CTGCATAACT TGGAGAGAAT CATCTGGTCG 
ATGCTGCTCA TCATTAGCAT CGGGCTGTCC TTCTTTTTCT ACCTCCTGCT CTCCGAGCGA 
TTCGTTTCCC AGAAGCTGCA GACGGTGGTC CACGATCCGC AGTTCCCCGT GTTTCTGGTG 
CCATTTCCGG CCGTGGGAAT CTGCACGGAC AACCGCATCA ACTGGAACAA GCTGGAGGCG 
GCCAAGGAGC AGTTCCTGCC GACCAATGCC AGCGTGGAAC TGGTCGAAAG TTTCACCGTC 
CTTGTTTCTC GGATGGAAAC TTTGCGCTTT GGCAGTTACC TTTCAAGCTT GGCCGAGCTG 
GAGGACGATA ACCTGGAGGC GGTGGGCTTT GTGAACCTAA CTGAGCTGGC CATATTTATG 
ACTCTTCAAT GCTCGGATAT CATGGTACCG AAATCCTGCC TGTGGCGCAG TTCCTCCTTC 
AATTGCTGCG AATACTTCGT GTTGGAGAAG ACGGAGTTTG GATTTTGTTT GGTTTTCAAC 
TCGGAGGTTT CGCCACGCTC CAAGGCAATT AAGCAGAAGG AGGGTAATAA CTTCTATCCG 
AGGCACAATG CCAAAGCAGG CCAGAGTACG GGCTTGAATT TTGATCTAAT CTTAAACGAG 
AGCTTCAGGC GACCCGATTC GCAGGCGAAT AATAATGTCT ACGTAAGTAT CTGCCAGGCC 
CCCGACCAAC TGAACAATGT CGTATATTCA ATAACCCAAA ACACGGAGAC CTACGTGACT 
GTCCGTCCGG GTCTCACTTG GACGGATAAC ACCACGCGCA GTATCCCGCC GGAGCGCAGG 
AACTGCCTCT TCGCGGACGA GCAGGGCGAA CTGGACGCCA ACGATTCGGC CAAGAACTTT 
GGAAAGCCCT TCCAGCTCTC CAACTGTCTC AACCGCTGCC ATGAGTCCTA CCTAATTCAG 
CTCTGCAACT GCTCCTTGCC CATTTTCTTC CTGTACAACC ACAGAGTTCC GGATTGCAAC 
GCAGTCAGCC TGCGCTGCCT TGCGCGTCAC AATGACATCT TCAGCTACGA CAAGCGAAGG 
GACGAGGATG CCCTTTTCAG TGCCACCAAG CTGGGAATGA CCTGTTCCTG CTTGGTGGAC 
TGCTACCTGC TCGACTATTA TACTTCCACC ACCACACTGC CTTTGTCAGC CCATAAACTA 
CCCAAGGATC CGCATCAGAA ACTCTTCAGG GTGGATGTGC ACTACCAAGT GGAGACCACA 
CCTTTGTATC GCACAAGCCT GGAGTTTACC ATTATCGATC TAATTGCCAA TCTGGGTGGA 
ATCTTTGGAC TTTGCTTGGG TGCTTCTATG GTCAGTGCCT TCGAGTTGAT CTACTACCTT 
ACTGTGGGAC TTGCTATGCA TCTATATGAC CACCAGTACT ATGGGGTCTT ATTTAAGCAC 
CTGAAGGCTA AGTGGGTCAA TTTAAAGGGC TACCTAAGGA ACGAAGTTGG CCATCTGGCT 
GAGAATCCCG CTGCCCACAA GGATACCAAT GACAGGAAAT TGAGGCATCC TTTTAACTAC 
AGAAAGAATG TGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263075.1
CDS	1..1698
Translation	MAKGDNSVKPTEAAGFGDNERCLADLLKVHFRSYCEKSTIHCVRYLYDSHLHNLERIIWSMLLIISIGLSFFFYLLLSERFVSQKLQTVVHDPQFPVFLVPFPAVGICTDNRINWNKLEAAKEQFLPTNASVELVESFTVLVSRMETLRFGSYLSSLAELEDDNLEAVGFVNLTELAIFMTLQCSDIMVPKSCLWRSSSFNCCEYFVLEKTEFGFCLVFNSEVSPRSKAIKQKEGNNFYPRHNAKAGQSTGLNFDLILNESFRRPDSQANNNVYVSICQAPDQLNNVVYSITQNTETYVTVRPGLTWTDNTTRSIPPERRNCLFADEQGELDANDSAKNFGKPFQLSNCLNRCHESYLIQLCNCSLPIFFLYNHRVPDCNAVSLRCLARHNDIFSYDKRRDEDALFSATKLGMTCSCLVDCYLLDYYTSTTTLPLSAHKLPKDPHQKLFRVDVHYQVETTPLYRTSLEFTIIDLIANLGGIFGLCLGASMVSAFELIYYLTVGLAMHLYDHQYYGVLFKHLKAKWVNLKGYLRNEVGHLAENPAAHKDTNDRKLRHPFNYRKNVW

Target ORF information:

RefSeq Version	NM_001276146.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pickpocket 20 (ppk20), transcript variant C, partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276146.1

ORF Insert Sequence:

ATGGCCAAGG GAGATAATTC GGTCAAACCG ACAGAGGCAG CCGGTTTTGG TGACAACGAG 
AGGTGCCTCG CGGATTTGTT AAAGGTCCAC TTTCGGAGCT ACTGCGAGAA GTCCACCATT 
CACTGCGTTC GCTATCTGTA CGATTCCCAC CTGCATAACT TGGAGAGAAT CATCTGGTCG 
ATGCTGCTCA TCATTAGCAT CGGGCTGTCC TTCTTTTTCT ACCTCCTGCT CTCCGAGCGA 
TTCGTTTCCC AGAAGCTGCA GACGGTGGTC CACGATCCGC AGTTCCCCGT GTTTCTGGTG 
CCATTTCCGG CCGTGGGAAT CTGCACGGAC AACCGCATCA ACTGGAACAA GCTGGAGGCG 
GCCAAGGAGC AGTTCCTGCC GACCAATGCC AGCGTGGAAC TGGTCGAAAG TTTCACCGTC 
CTTGTTTCTC GGATGGAAAC TTTGCGCTTT GGCAGTTACC TTTCAAGCTT GGCCGAGCTG 
GAGGACGATA ACCTGGAGGC GGTGGGCTTT GTGAACCTAA CTGAGCTGGC CATATTTATG 
ACTCTTCAAT GCTCGGATAT CATGGTACCG AAATCCTGCC TGTGGCGCAG TTCCTCCTTC 
AATTGCTGCG AATACTTCGT GTTGGAGAAG ACGGAGTTTG GATTTTGTTT GGTTTTCAAC 
TCGGAGGTTT CGCCACGCTC CAAGGCAATT AAGCAGAAGG AGGGTAATAA CTTCTATCCG 
AGGCACAATG CCAAAGCAGG CCAGAGTACG GGCTTGAATT TTGATCTAAT CTTAAACGAG 
AGCTTCAGGC GACCCGATTC GCAGGCGAAT AATAATGTCT ACGTAAGTAT CTGCCAGGCC 
CCCGACCAAC TGAACAATGT CGTATATTCA ATAACCCAAA ACACGGAGAC CTACGTGACT 
GTCCGTCCGG GTCTCACTTG GACGGATAAC ACCACGCGCA GTATCCCGCC GGAGCGCAGG 
AACTGCCTCT TCGCGGACGA GCAGGGCGAA CTGGACGCCA ACGATTCGGC CAAGAACTTT 
GGAAAGCCCT TCCAGCTCTC CAACTGTCTC AACCGCTGCC ATGAGTCCTA CCTAATTCAG 
CTCTGCAACT GCTCCTTGCC CATTTTCTTC CTGTACAACC ACAGAGTTCC GGATTGCAAC 
GCAGTCAGCC TGCGCTGCCT TGCGCGTCAC AATGACATCT TCAGCTACGA CAAGCGAAGG 
GACGAGGATG CCCTTTTCAG TGCCACCAAG CTGGGAATGA CCTGTTCCTG CTTGGTGGAC 
TGCTACCTGC TCGACTATTA TACTTCCACC ACCACACTGC CTTTGTCAGC CCATAAACTA 
CCCAAGGATC CGCATCAGAA ACTCTTCAGG GTGGATGTGC ACTACCAAGT GGAGACCACA 
CCTTTGTATC GCACAAGCCT GGAGTTTACC ATTATCGATC TAATTGCCAA TCTGGGTGGA 
ATCTTTGGAC TTTGCTTGGG TGCTTCTATG GTCAGTGCCT TCGAGTTGAT CTACTACCTT 
ACTGTGGGAC TTGCTATGCA TCTATATGAC CACCAGTACT ATGGGGTCTT ATTTAAGCAC 
CTGAAGGCTA AGTGGGTCAA TTTAAAGGGC TACCTAAGGA ACGAAGTTGG CCATCTGGCT 
GAGAATCCCG CTGCCCACAA GGATACCAAT GACAGGAAAT TGAGGCATCC TTTTAACTAC 
AGAAAGAATG TGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ppk20 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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