dgt6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dgt6
Entrez Gene ID	43441
Full Name	dim gamma-tubulin 6
Synonyms	CG11881,Dgt6,Dmel\CG11881,msd4
General protein information	Preferred Names dim gamma-tubulin 6 Names CG11881 gene product from transcript CG11881-RA CG11881-PA dgt6-PA mitotic spindle density 4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F12-98F12

mRNA and Protein(s)

mRNA	Protein	Name
NM_143414.4	NP_651671.1	dim gamma-tubulin 6

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dgt6 plays a pivotal role in kinetochore-driven k-fiber formation, mediating nucleation and/or initial stabilization of chromosome-induced microtubules.
Title: Drosophila Dgt6 interacts with Ndc80, Msps/XMAP215, and gamma-tubulin to promote kinetochore-driven MT formation.

Results suggest that an important mitotic function for gamma-tubulin may lie within the spindle, where augmin (Dgt2-6) and gamma-tubulin function cooperatively to amplify the number of microtubules.
Title: Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle.

The following dgt6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgt6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06462	NM_143414.4 Latest version!	Drosophila melanogaster dim gamma-tubulin 6 (dgt6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dgt6 ( NM_143414.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651671.1 Drosophila melanogaster dim gamma-tubulin 6 (dgt6), mRNA.

Price & Availability↑

CloneID	OFa06462
Clone ID Related Accession (Same CDS sequence)	NM_143414.4
Accession Version	NM_143414.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dim gamma-tubulin 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143414.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGGA CCATAATTGC ACCGTGGAAG GCCGAAGAGA AGGAGCAGAG CGAGAAGCTC 
CACAGGAAGC TGCAGGGACT GGCCCTGGTG CACCCCCTGC CGGACGAGCT GCGTAAGCTG 
ATTGCCTGGG ACATGTTCCT CAAGCCCAAC CAGGTGGCCT TCGTGCATGT GATGCACTAC 
CTCTTCCGTC TGCTGGATCC CGCGGAGTTC AAACGCCGCT TCTTCTGGCC CATCACCGAC 
AAGAAGTCGG AGGCGAACTT TCGCTCCAGC ACGGTGGAGT ATCTGAAGCA TCTGAACGAG 
AAGCACCAGC TGCATTGGGC CAACATCAAG TCGTATCTGG TGGTGATGCC CGGAGGCATG 
AGGTTCATCA ACTTCCTGCT CGAGTTCGTG GGCTTCGTGA TCCAGGAGCT CATCAAGCAG 
CGCGAGAAGT CACTTGGCTT AGAGGCGGGA ACACCAAACG TGAGCGCCAA GGTCATGGCC 
CGCCAAAATG CCGTGATGAA GGAGTACGCT TCTTCGTATG TGGTCAATCT GGAGGAGAAT 
ACGGCCCTGT TGCGGGACAA GACCCAGAAG ATCAGACGCC TGATGGCCGA TCTGTCTGCA 
GACATGGGAG TGCCCGAGGA GCAACTGGCG GACGATGGCT TTCTGGATGA GTTCGAGGCC 
ACCGCTGCAC TCGGCGTGGA GCGGGTCATT ACCCAACCCA CCGAAAGGAA ATTCGACCTG 
GAGGCATCTC TGTGCGGTCT CAAGGAGGCC ATCGATCTTT TCCAGGTGAA GCAAGCCGAG 
AACAACCAGA GCAAGGAGGC GGTGGAGAAG GCACTTCGGG GCATGCGAGT GCTGTTCGAC 
TGTGATGCAG TCACAGAAGG AGATTTCTAC GATCCTTTGG GCGCATCAAA AATGGATGCG 
CTCTTAAACG GCTTTAATCG GATCAGCGGC ACAATTGCCG AGCAACTGGA TGCTAATGAC 
CACTACAACG AATCGAATGC GTTTGTCACC ACCGATTTGC AGGCTCTTCG AGTGGAACTT 
TCACAGTCCG AAGTCCAATT GAACAATTTA CTCAAGAAAC TAAACGAACC GTCCAAAAAG 
GACAAGGGAA GTGCCAATGC AAGTGGCTCC GCCAGGGTAC AGACGCTTCA GCCGGCCACG 
CCTCGCTTCG AATCGGTTAT CAGCTCCAAA TTTGTTTCCA CGCCTCCCAT CAGAATCGAC 
ATGGGCGGCG GTGGCGGTCG CAATGCCCCA GTGCGTCTTG CACTGCAAGA TGACTTCAAT 
GGCAAACAGT TTGATGCCTT AAGCAACAGC TTACTCGCAC CTGCGCCGCC TCGATCAGCA 
CGCAAGCTGA AAGCCCTTGA TCAATCGACC GGCATTGACC TTAATGGCAC TTTAAACCGA 
TCCAAGATCA ACGATCCCAT GCAAATGCTG CGCACCATTC ACAAAAACAC CTCCAAGGTC 
AAGGCAGCTC CGCAGGCGAA TCTCTCCTCC CTGGGCAGCA AGTGGAAGCA AATGCAAGCG 
TCATTCGGTT TCGATGAGGC GCCGGTGCCA GGAGCAGCAG TGATCTCACC TCAGACCTCA 
CCAACGGATC CCAGCGATCC CTTTACACCC TTGAGTGGTA GCGAGTGCAC CCGAATTGAG 
CGCATTCCGC GCACTTCGGA AAACAACACT TCACTGATCG CCAAGAGCGC TGCGGTTATA 
AAGGTCCTGG AGGTATCACG GAATGTGCTT AATCTTAGCA CCTCGCCATC TGGACGCCTA 
GATGCACTGG TTCCACATAC CGAGAGCCAC CAGCAAGATG TCGCGCCACG TCTTCAGCTC 
AACGACAGAA CAATCAATGA CTCACTGCAG CTTGATCCCG ATTTCAACAT TGAAAACGAT 
AAGAATGCAT TCAATGTCTC GCATAAATTC GATTTTGATC AGATTGGTGG TGAGGATGAC 
CTGCAGAACA TCAGCGATAG CGTGCTCAAG GATATTATCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651671.1
CDS	82..2046
Translation	MDRTIIAPWKAEEKEQSEKLHRKLQGLALVHPLPDELRKLIAWDMFLKPNQVAFVHVMHYLFRLLDPAEFKRRFFWPITDKKSEANFRSSTVEYLKHLNEKHQLHWANIKSYLVVMPGGMRFINFLLEFVGFVIQELIKQREKSLGLEAGTPNVSAKVMARQNAVMKEYASSYVVNLEENTALLRDKTQKIRRLMADLSADMGVPEEQLADDGFLDEFEATAALGVERVITQPTERKFDLEASLCGLKEAIDLFQVKQAENNQSKEAVEKALRGMRVLFDCDAVTEGDFYDPLGASKMDALLNGFNRISGTIAEQLDANDHYNESNAFVTTDLQALRVELSQSEVQLNNLLKKLNEPSKKDKGSANASGSARVQTLQPATPRFESVISSKFVSTPPIRIDMGGGGGRNAPVRLALQDDFNGKQFDALSNSLLAPAPPRSARKLKALDQSTGIDLNGTLNRSKINDPMQMLRTIHKNTSKVKAAPQANLSSLGSKWKQMQASFGFDEAPVPGAAVISPQTSPTDPSDPFTPLSGSECTRIERIPRTSENNTSLIAKSAAVIKVLEVSRNVLNLSTSPSGRLDALVPHTESHQQDVAPRLQLNDRTINDSLQLDPDFNIENDKNAFNVSHKFDFDQIGGEDDLQNISDSVLKDIIF

Target ORF information:

RefSeq Version	NM_143414.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dim gamma-tubulin 6 (dgt6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143414.4

ORF Insert Sequence:

ATGGATCGGA CCATAATTGC ACCGTGGAAG GCCGAAGAGA AGGAGCAGAG CGAGAAGCTC 
CACAGGAAGC TGCAGGGACT GGCCCTGGTG CACCCCCTGC CGGACGAGCT GCGTAAGCTG 
ATTGCCTGGG ACATGTTCCT CAAGCCCAAC CAGGTGGCCT TCGTGCATGT GATGCACTAC 
CTCTTCCGTC TGCTGGATCC CGCGGAGTTC AAACGCCGCT TCTTCTGGCC CATCACCGAC 
AAGAAGTCGG AGGCGAACTT TCGCTCCAGC ACGGTGGAGT ATCTGAAGCA TCTGAACGAG 
AAGCACCAGC TGCATTGGGC CAACATCAAG TCGTATCTGG TGGTGATGCC CGGAGGCATG 
AGGTTCATCA ACTTCCTGCT CGAGTTCGTG GGCTTCGTGA TCCAGGAGCT CATCAAGCAG 
CGCGAGAAGT CACTTGGCTT AGAGGCGGGA ACACCAAACG TGAGCGCCAA GGTCATGGCC 
CGCCAAAATG CCGTGATGAA GGAGTACGCT TCTTCGTATG TGGTCAATCT GGAGGAGAAT 
ACGGCCCTGT TGCGGGACAA GACCCAGAAG ATCAGACGCC TGATGGCCGA TCTGTCTGCA 
GACATGGGAG TGCCCGAGGA GCAACTGGCG GACGATGGCT TTCTGGATGA GTTCGAGGCC 
ACCGCTGCAC TCGGCGTGGA GCGGGTCATT ACCCAACCCA CCGAAAGGAA ATTCGACCTG 
GAGGCATCTC TGTGCGGTCT CAAGGAGGCC ATCGATCTTT TCCAGGTGAA GCAAGCCGAG 
AACAACCAGA GCAAGGAGGC GGTGGAGAAG GCACTTCGGG GCATGCGAGT GCTGTTCGAC 
TGTGATGCAG TCACAGAAGG AGATTTCTAC GATCCTTTGG GCGCATCAAA AATGGATGCG 
CTCTTAAACG GCTTTAATCG GATCAGCGGC ACAATTGCCG AGCAACTGGA TGCTAATGAC 
CACTACAACG AATCGAATGC GTTTGTCACC ACCGATTTGC AGGCTCTTCG AGTGGAACTT 
TCACAGTCCG AAGTCCAATT GAACAATTTA CTCAAGAAAC TAAACGAACC GTCCAAAAAG 
GACAAGGGAA GTGCCAATGC AAGTGGCTCC GCCAGGGTAC AGACGCTTCA GCCGGCCACG 
CCTCGCTTCG AATCGGTTAT CAGCTCCAAA TTTGTTTCCA CGCCTCCCAT CAGAATCGAC 
ATGGGCGGCG GTGGCGGTCG CAATGCCCCA GTGCGTCTTG CACTGCAAGA TGACTTCAAT 
GGCAAACAGT TTGATGCCTT AAGCAACAGC TTACTCGCAC CTGCGCCGCC TCGATCAGCA 
CGCAAGCTGA AAGCCCTTGA TCAATCGACC GGCATTGACC TTAATGGCAC TTTAAACCGA 
TCCAAGATCA ACGATCCCAT GCAAATGCTG CGCACCATTC ACAAAAACAC CTCCAAGGTC 
AAGGCAGCTC CGCAGGCGAA TCTCTCCTCC CTGGGCAGCA AGTGGAAGCA AATGCAAGCG 
TCATTCGGTT TCGATGAGGC GCCGGTGCCA GGAGCAGCAG TGATCTCACC TCAGACCTCA 
CCAACGGATC CCAGCGATCC CTTTACACCC TTGAGTGGTA GCGAGTGCAC CCGAATTGAG 
CGCATTCCGC GCACTTCGGA AAACAACACT TCACTGATCG CCAAGAGCGC TGCGGTTATA 
AAGGTCCTGG AGGTATCACG GAATGTGCTT AATCTTAGCA CCTCGCCATC TGGACGCCTA 
GATGCACTGG TTCCACATAC CGAGAGCCAC CAGCAAGATG TCGCGCCACG TCTTCAGCTC 
AACGACAGAA CAATCAATGA CTCACTGCAG CTTGATCCCG ATTTCAACAT TGAAAACGAT 
AAGAATGCAT TCAATGTCTC GCATAAATTC GATTTTGATC AGATTGGTGG TGAGGATGAC 
CTGCAGAACA TCAGCGATAG CGTGCTCAAG GATATTATCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dgt6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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