yem cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	yem
Entrez Gene ID	43439
Full Name	yemanuclein
Synonyms	CG11879,CG14513,Dmel\CG14513,Yemalpha,yG4.5,yT4.5,yem-alpha,yema,yemalpha
General protein information	Preferred Names yemanuclein Names CG14513 gene product from transcript CG14513-RA CG14513-PA CG14513-PB yem-PA yem-PB yem-alpha yema nuclein alpha yema-gene-4.5 yemalpha yemanuclein alpha yemanuclein-alpha
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F10-98F10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276125.1	NP_001263054.1	yemanuclein, isoform B
NM_057545.4	NP_476893.1	yemanuclein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Intellectual disability-associated BRWD3 regulates gene expression through inhibition of HIRA/YEM-mediated chromatin deposition of histone H3.3.
Title: Intellectual disability-associated dBRWD3 regulates gene expression through inhibition of HIRA/YEM-mediated chromatin deposition of histone H3.3.

Drosophila Yemanuclein associates with the cohesin and synaptonemal complexes
Title: Drosophila Yemanuclein associates with the cohesin and synaptonemal complexes.

we demonstrate that HIRA and YEM proteins interact and are mutually dependent for their targeting to the decondensing male pronucleus.
Title: Drosophila Yemanuclein and HIRA cooperate for de novo assembly of H3.3-containing nucleosomes in the male pronucleus.

The following yem gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the yem cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06460	NM_057545.4 Latest version!	Drosophila melanogaster yemanuclein (yem), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa08941	NM_001276125.1 Latest version!	Drosophila melanogaster yemanuclein (yem), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

yem ( NM_057545.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476893.1 Drosophila melanogaster yemanuclein (yem), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06460
Clone ID Related Accession (Same CDS sequence)	NM_057545.4
Accession Version	NM_057545.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	yemanuclein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057545.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAAAGG GGGGCGAGCA CAAGCGCGTC ACCCTCACCT CGATCATCCA GGGCGATGCG 
GGCTTCTCGC GCTTCGGCAG CAACATCCTG GAGCCTGATG CTGCGTCCGC TGCACCCGAC 
AACAGCTCCA AACCCACGAC AAAGACTGCC AAGTGCATCC GCATCAAACT CGATCTGTTC 
GAGACCGATT CGAACAAGTA TCCCGAGTTT AACTACTCCC GGCTTTTGTA TTTGGAAAAG 
AAAAAGACCA AAAAGCTGAA GCAGGTGAGC ACTACCAATG GCTCCGCAAG CACTGATCCC 
TTTGCCGACA ACGATGACGA TGTGGCACGG ATCGTCAAGG AGCTGGAGGC CAAGTATGGC 
AACTCCTATG CCACAGGCAG GGGGAAGAGC AAAAAGGACG ACTACCGGGA CATTGGAATG 
GGCTACGATG AGTCGGACTC ATTCATCGAC AACACGGAAG CCTACGACGA AATCATACCG 
GAGGAGGCGG AAACTCTGGA GGGCGGCTTC TACATCAACT GTGGCGCACT GGAGTTCAAG 
AACCTCACCA AGAAGTCCTA CACCACTCGA ACAGATGCCA TCATCAAAAT GCCAGAGCGA 
TCACGCAAGC GCATGGTCTC GTCCAGCTCG GAAAGCTCAT CGTCATCCTC GGGCGATGAT 
GACGAAAACG ATGATGGCAA CAATGAGGAG GACGACGAGA GCGATTCCGA GGACGACTCC 
GAGGAGAACG ATGAAAGCGA CTCCGAAGAC GACTCCGAGA GTGAAAGTCT TGAGGACGAG 
GACTCTGCTG CTACAGCCAA ATCCAGCAGC AAGTACAAAG ACAATCATCA AGCTAAGCGG 
GCCAAGGTCA TCGTTACGGG CAAGTCCAAA CCAAGCTCAA GTTCTTTGAC GAGTGGCAAG 
AAGCCGCCGA CTAAGCCGAT CACCACTTCC TCATCCTCAA ACTCGCCACG ACCCAGCACG 
GTGGAGATTT CGGACACGGA AGACGGCCAA GACCCTATCC AGACTCAGCC ATCAAGTCAG 
CTTCAGTCCC TGCCGCAGAG TCAGGCCCAG GCGCAAGCTT TAAAGAAGGT GGTTAAGACC 
ACAACGGTCA AGGACATGCT AAAGGCCAAG CGAGACAGCT TCCTGAAGTC GCAGAGCGGC 
ACGGCAGCTG TCAAGGGCGT CGGCAACGGC GAGCTGAAGT GTGTTTCCAC CGACGTGTCC 
AGCAGCGATA GCAGCGATAT GGAGTCTGAG CACGGCCGGG CAGATAGGCA GGCCGGTCAG 
CATGGCAAGG ATGGGCAGGA AAATCTTCGC ACCGCCGACA CTCTGCTCCC CACCACATTG 
GATGCGGACA TTGTGACCGC AGTCAACAGC TTCAAAGAAG CGGTGAAGAG TCGAGACATG 
TGCGGCAAGA AGTTTAATCT GGATGTGAAG CTTTCGCCAC TACTGCTTCG GGTGTACGAG 
GCGGTGCTGT GCACGGACCG CAACGAGCGC AACATGGTCT TCTCCCACAT CGAGTATCAG 
CTGCAACTGC CCAAGTACTA CATGCTGCGC AAAGGGAAAC AAGTGCGGGC GAAGGAGGAG 
AAGAGGAAGT CCACCATTAT GCTGGAGAAG CTGCGGCGGG CTGTAGCTGT GGTCATGCCT 
AAGGCGGTGG CCAACTATGA GACGGAACTG CGCACATTTG CTGAACAGGC AGCTGCGGAC 
GTGAACTCCG AACTGCCGCC CAAAATGCCG CGTAAGAAGT TCCAGTGGAC CAGCGAGCTA 
CGCCATCTGC TGTACGATGT GTACCAAGCG CGTTGGACGT CCTACGCTTT CCTAGCCAAG 
CGCAAGGAAT CGTTGGAGGA GTTCATCAAT TGGTATCTCA AGGAAAAGGT GGTGGAACTG 
TGGCCGCCAG GCTGGATGCG TTTGGATGAG TTGCAAAGGG AAATAACACG CTACAAGAAC 
GCCAAGCTGA AAGCAAAGGA GAAGCCAAAG GCACCGCCGG CGTCCGCATC GCCCAAGCCC 
GTTGGAGTTG TATCTGCTCC TGAGCAAATG CCGCCGGCAA GCAGCTACCT AAAGGCAGTG 
GAGGACCCTC GATCTCGAGG TAACTCCGAT ACGGACTCAG CTACAAGTGC CTCCTCCAAC 
AGTCTGAAGC GAAAGCTGAA GGAGATGCCC AAGCAGACCA GCAAGCCGCC AAAGAAAAAG 
GTGGCTAAGC AGGTACCGCT GCAACCGCAA CTTACTCCGC ATCCGCAATT CCAGCTGGCG 
CCGGCTGCCA CGGCGGCCGT CAGTATACCA GCCATTAGCA ATAACAACAA CCACCTGCCC 
CACCTGGACA CGCTGCTCTC CATGCCGAGC ACCTCGGCTC AGGCAGCAGC ACTCAATGCC 
GCCGCCGTAG CAGCCGCCAG TACGGTTCTG GATCTGGCAT CCCCTAGCCG GAAAATTGAC 
CTTAACACCA GCAACAATTT CTACAATCTG ATCACGGCCG CCTCCCTGGC AGCCAGCGGG 
AATCCCAGTC CGCATTCTGG AGACGGCCAA GCCAAGGTGA TTGTGGGAGC TCGGCCCTCT 
CCCCATGTGA TCAACCTGGA TGACTATCAA TGCACCAGCG ATATACTTCA GACGTCCAAG 
CAGCTAGCTG CCACCACCAC TGTCATCACC GCCATCTCAA AGGCCGCGCA AACGACGTCG 
GTAGCCAGGG AAAGCAGCAG CGAGTCGGAT GGCGTTGAGA TTGTGGGAGT TTTTCCTGCC 
TCTAAGCCCC AAAAGAAAGT CCAGTCTAAG CCCAAGAACA AGACTCAGAA CAGAGGTCGG 
TCGTCTTTGG GTGCGGTGGG GCAGGTAAAT GGATCCCTGG GCTACAGCGC GAATAACATG 
TATATCTACA ATAGCCCAAG AACGCTGGGT CCAGTGTACG ATCTCACCGA TCCGCATATA 
ATGAAAACTA TGTCAAATCT GAAGGAAATG GAAAAGCAAT TCATTCAAGC GGCTTTCTCG 
CCGAACAGCG TCAAGGGCGC AAGCGGAGGA ATGGGATCCT CAGCGCCCAG TACGCCCACG 
CGCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476893.1
CDS	679..3687
Translation	MSKGGEHKRVTLTSIIQGDAGFSRFGSNILEPDAASAAPDNSSKPTTKTAKCIRIKLDLFETDSNKYPEFNYSRLLYLEKKKTKKLKQVSTTNGSASTDPFADNDDDVARIVKELEAKYGNSYATGRGKSKKDDYRDIGMGYDESDSFIDNTEAYDEIIPEEAETLEGGFYINCGALEFKNLTKKSYTTRTDAIIKMPERSRKRMVSSSSESSSSSSGDDDENDDGNNEEDDESDSEDDSEENDESDSEDDSESESLEDEDSAATAKSSSKYKDNHQAKRAKVIVTGKSKPSSSSLTSGKKPPTKPITTSSSSNSPRPSTVEISDTEDGQDPIQTQPSSQLQSLPQSQAQAQALKKVVKTTTVKDMLKAKRDSFLKSQSGTAAVKGVGNGELKCVSTDVSSSDSSDMESEHGRADRQAGQHGKDGQENLRTADTLLPTTLDADIVTAVNSFKEAVKSRDMCGKKFNLDVKLSPLLLRVYEAVLCTDRNERNMVFSHIEYQLQLPKYYMLRKGKQVRAKEEKRKSTIMLEKLRRAVAVVMPKAVANYETELRTFAEQAAADVNSELPPKMPRKKFQWTSELRHLLYDVYQARWTSYAFLAKRKESLEEFINWYLKEKVVELWPPGWMRLDELQREITRYKNAKLKAKEKPKAPPASASPKPVGVVSAPEQMPPASSYLKAVEDPRSRGNSDTDSATSASSNSLKRKLKEMPKQTSKPPKKKVAKQVPLQPQLTPHPQFQLAPAATAAVSIPAISNNNNHLPHLDTLLSMPSTSAQAAALNAAAVAAASTVLDLASPSRKIDLNTSNNFYNLITAASLAASGNPSPHSGDGQAKVIVGARPSPHVINLDDYQCTSDILQTSKQLAATTTVITAISKAAQTTSVARESSSESDGVEIVGVFPASKPQKKVQSKPKNKTQNRGRSSLGAVGQVNGSLGYSANNMYIYNSPRTLGPVYDLTDPHIMKTMSNLKEMEKQFIQAAFSPNSVKGASGGMGSSAPSTPTRQ

Target ORF information:

RefSeq Version	NM_057545.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster yemanuclein (yem), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057545.4

ORF Insert Sequence:

ATGTCAAAGG GGGGCGAGCA CAAGCGCGTC ACCCTCACCT CGATCATCCA GGGCGATGCG 
GGCTTCTCGC GCTTCGGCAG CAACATCCTG GAGCCTGATG CTGCGTCCGC TGCACCCGAC 
AACAGCTCCA AACCCACGAC AAAGACTGCC AAGTGCATCC GCATCAAACT CGATCTGTTC 
GAGACCGATT CGAACAAGTA TCCCGAGTTT AACTACTCCC GGCTTTTGTA TTTGGAAAAG 
AAAAAGACCA AAAAGCTGAA GCAGGTGAGC ACTACCAATG GCTCCGCAAG CACTGATCCC 
TTTGCCGACA ACGATGACGA TGTGGCACGG ATCGTCAAGG AGCTGGAGGC CAAGTATGGC 
AACTCCTATG CCACAGGCAG GGGGAAGAGC AAAAAGGACG ACTACCGGGA CATTGGAATG 
GGCTACGATG AGTCGGACTC ATTCATCGAC AACACGGAAG CCTACGACGA AATCATACCG 
GAGGAGGCGG AAACTCTGGA GGGCGGCTTC TACATCAACT GTGGCGCACT GGAGTTCAAG 
AACCTCACCA AGAAGTCCTA CACCACTCGA ACAGATGCCA TCATCAAAAT GCCAGAGCGA 
TCACGCAAGC GCATGGTCTC GTCCAGCTCG GAAAGCTCAT CGTCATCCTC GGGCGATGAT 
GACGAAAACG ATGATGGCAA CAATGAGGAG GACGACGAGA GCGATTCCGA GGACGACTCC 
GAGGAGAACG ATGAAAGCGA CTCCGAAGAC GACTCCGAGA GTGAAAGTCT TGAGGACGAG 
GACTCTGCTG CTACAGCCAA ATCCAGCAGC AAGTACAAAG ACAATCATCA AGCTAAGCGG 
GCCAAGGTCA TCGTTACGGG CAAGTCCAAA CCAAGCTCAA GTTCTTTGAC GAGTGGCAAG 
AAGCCGCCGA CTAAGCCGAT CACCACTTCC TCATCCTCAA ACTCGCCACG ACCCAGCACG 
GTGGAGATTT CGGACACGGA AGACGGCCAA GACCCTATCC AGACTCAGCC ATCAAGTCAG 
CTTCAGTCCC TGCCGCAGAG TCAGGCCCAG GCGCAAGCTT TAAAGAAGGT GGTTAAGACC 
ACAACGGTCA AGGACATGCT AAAGGCCAAG CGAGACAGCT TCCTGAAGTC GCAGAGCGGC 
ACGGCAGCTG TCAAGGGCGT CGGCAACGGC GAGCTGAAGT GTGTTTCCAC CGACGTGTCC 
AGCAGCGATA GCAGCGATAT GGAGTCTGAG CACGGCCGGG CAGATAGGCA GGCCGGTCAG 
CATGGCAAGG ATGGGCAGGA AAATCTTCGC ACCGCCGACA CTCTGCTCCC CACCACATTG 
GATGCGGACA TTGTGACCGC AGTCAACAGC TTCAAAGAAG CGGTGAAGAG TCGAGACATG 
TGCGGCAAGA AGTTTAATCT GGATGTGAAG CTTTCGCCAC TACTGCTTCG GGTGTACGAG 
GCGGTGCTGT GCACGGACCG CAACGAGCGC AACATGGTCT TCTCCCACAT CGAGTATCAG 
CTGCAACTGC CCAAGTACTA CATGCTGCGC AAAGGGAAAC AAGTGCGGGC GAAGGAGGAG 
AAGAGGAAGT CCACCATTAT GCTGGAGAAG CTGCGGCGGG CTGTAGCTGT GGTCATGCCT 
AAGGCGGTGG CCAACTATGA GACGGAACTG CGCACATTTG CTGAACAGGC AGCTGCGGAC 
GTGAACTCCG AACTGCCGCC CAAAATGCCG CGTAAGAAGT TCCAGTGGAC CAGCGAGCTA 
CGCCATCTGC TGTACGATGT GTACCAAGCG CGTTGGACGT CCTACGCTTT CCTAGCCAAG 
CGCAAGGAAT CGTTGGAGGA GTTCATCAAT TGGTATCTCA AGGAAAAGGT GGTGGAACTG 
TGGCCGCCAG GCTGGATGCG TTTGGATGAG TTGCAAAGGG AAATAACACG CTACAAGAAC 
GCCAAGCTGA AAGCAAAGGA GAAGCCAAAG GCACCGCCGG CGTCCGCATC GCCCAAGCCC 
GTTGGAGTTG TATCTGCTCC TGAGCAAATG CCGCCGGCAA GCAGCTACCT AAAGGCAGTG 
GAGGACCCTC GATCTCGAGG TAACTCCGAT ACGGACTCAG CTACAAGTGC CTCCTCCAAC 
AGTCTGAAGC GAAAGCTGAA GGAGATGCCC AAGCAGACCA GCAAGCCGCC AAAGAAAAAG 
GTGGCTAAGC AGGTACCGCT GCAACCGCAA CTTACTCCGC ATCCGCAATT CCAGCTGGCG 
CCGGCTGCCA CGGCGGCCGT CAGTATACCA GCCATTAGCA ATAACAACAA CCACCTGCCC 
CACCTGGACA CGCTGCTCTC CATGCCGAGC ACCTCGGCTC AGGCAGCAGC ACTCAATGCC 
GCCGCCGTAG CAGCCGCCAG TACGGTTCTG GATCTGGCAT CCCCTAGCCG GAAAATTGAC 
CTTAACACCA GCAACAATTT CTACAATCTG ATCACGGCCG CCTCCCTGGC AGCCAGCGGG 
AATCCCAGTC CGCATTCTGG AGACGGCCAA GCCAAGGTGA TTGTGGGAGC TCGGCCCTCT 
CCCCATGTGA TCAACCTGGA TGACTATCAA TGCACCAGCG ATATACTTCA GACGTCCAAG 
CAGCTAGCTG CCACCACCAC TGTCATCACC GCCATCTCAA AGGCCGCGCA AACGACGTCG 
GTAGCCAGGG AAAGCAGCAG CGAGTCGGAT GGCGTTGAGA TTGTGGGAGT TTTTCCTGCC 
TCTAAGCCCC AAAAGAAAGT CCAGTCTAAG CCCAAGAACA AGACTCAGAA CAGAGGTCGG 
TCGTCTTTGG GTGCGGTGGG GCAGGTAAAT GGATCCCTGG GCTACAGCGC GAATAACATG 
TATATCTACA ATAGCCCAAG AACGCTGGGT CCAGTGTACG ATCTCACCGA TCCGCATATA 
ATGAAAACTA TGTCAAATCT GAAGGAAATG GAAAAGCAAT TCATTCAAGC GGCTTTCTCG 
CCGAACAGCG TCAAGGGCGC AAGCGGAGGA ATGGGATCCT CAGCGCCCAG TACGCCCACG 
CGCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

yem ( NM_001276125.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263054.1 Drosophila melanogaster yemanuclein (yem), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08941
Clone ID Related Accession (Same CDS sequence)	NM_001276125.1
Accession Version	NM_001276125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	yemanuclein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAAAGG GGGGCGAGCA CAAGCGCGTC ACCCTCACCT CGATCATCCA GGGCGATGCG 
GGCTTCTCGC GCTTCGGCAG CAACATCCTG GAGCCTGATG CTGCGTCCGC TGCACCCGAC 
AACAGCTCCA AACCCACGAC AAAGACTGCC AAGTGCATCC GCATCAAACT CGATCTGTTC 
GAGACCGATT CGAACAAGTA TCCCGAGTTT AACTACTCCC GGCTTTTGTA TTTGGAAAAG 
AAAAAGACCA AAAAGCTGAA GCAGGTGAGC ACTACCAATG GCTCCGCAAG CACTGATCCC 
TTTGCCGACA ACGATGACGA TGTGGCACGG ATCGTCAAGG AGCTGGAGGC CAAGTATGGC 
AACTCCTATG CCACAGGCAG GGGGAAGAGC AAAAAGGACG ACTACCGGGA CATTGGAATG 
GGCTACGATG AGTCGGACTC ATTCATCGAC AACACGGAAG CCTACGACGA AATCATACCG 
GAGGAGGCGG AAACTCTGGA GGGCGGCTTC TACATCAACT GTGGCGCACT GGAGTTCAAG 
AACCTCACCA AGAAGTCCTA CACCACTCGA ACAGATGCCA TCATCAAAAT GCCAGAGCGA 
TCACGCAAGC GCATGGTCTC GTCCAGCTCG GAAAGCTCAT CGTCATCCTC GGGCGATGAT 
GACGAAAACG ATGATGGCAA CAATGAGGAG GACGACGAGA GCGATTCCGA GGACGACTCC 
GAGGAGAACG ATGAAAGCGA CTCCGAAGAC GACTCCGAGA GTGAAAGTCT TGAGGACGAG 
GACTCTGCTG CTACAGCCAA ATCCAGCAGC AAGTACAAAG ACAATCATCA AGCTAAGCGG 
GCCAAGGTCA TCGTTACGGG CAAGTCCAAA CCAAGCTCAA GTTCTTTGAC GAGTGGCAAG 
AAGCCGCCGA CTAAGCCGAT CACCACTTCC TCATCCTCAA ACTCGCCACG ACCCAGCACG 
GTGGAGATTT CGGACACGGA AGACGGCCAA GACCCTATCC AGACTCAGCC ATCAAGTCAG 
CTTCAGTCCC TGCCGCAGAG TCAGGCCCAG GCGCAAGCTT TAAAGAAGGT GGTTAAGACC 
ACAACGGTCA AGGACATGCT AAAGGCCAAG CGAGACAGCT TCCTGAAGTC GCAGAGCGGC 
ACGGCAGCTG TCAAGGGCGT CGGCAACGGC GAGCTGAAGT GTGTTTCCAC CGACGTGTCC 
AGCAGCGATA GCAGCGATAT GGAGTCTGAG CACGGCCGGG CAGATAGGCA GGCCGGTCAG 
CATGGCAAGG ATGGGCAGGA AAATCTTCGC ACCGCCGACA CTCTGCTCCC CACCACATTG 
GATGCGGACA TTGTGACCGC AGTCAACAGC TTCAAAGAAG CGGTGAAGAG TCGAGACATG 
TGCGGCAAGA AGTTTAATCT GGATGTGAAG CTTTCGCCAC TACTGCTTCG GGTGTACGAG 
GCGGTGCTGT GCACGGACCG CAACGAGCGC AACATGGTCT TCTCCCACAT CGAGTATCAG 
CTGCAACTGC CCAAGTACTA CATGCTGCGC AAAGGGAAAC AAGTGCGGGC GAAGGAGGAG 
AAGAGGAAGT CCACCATTAT GCTGGAGAAG CTGCGGCGGG CTGTAGCTGT GGTCATGCCT 
AAGGCGGTGG CCAACTATGA GACGGAACTG CGCACATTTG CTGAACAGGC AGCTGCGGAC 
GTGAACTCCG AACTGCCGCC CAAAATGCCG CGTAAGAAGT TCCAGTGGAC CAGCGAGCTA 
CGCCATCTGC TGTACGATGT GTACCAAGCG CGTTGGACGT CCTACGCTTT CCTAGCCAAG 
CGCAAGGAAT CGTTGGAGGA GTTCATCAAT TGGTATCTCA AGGAAAAGGT GGTGGAACTG 
TGGCCGCCAG GCTGGATGCG TTTGGATGAG TTGCAAAGGG AAATAACACG CTACAAGAAC 
GCCAAGCTGA AAGCAAAGGA GAAGCCAAAG GCACCGCCGG CGTCCGCATC GCCCAAGCCC 
GTTGGAGTTG TATCTGCTCC TGAGCAAATG CCGCCGGCAA GCAGCTACCT AAAGGCAGTG 
GAGGACCCTC GATCTCGAGG TAACTCCGAT ACGGACTCAG CTACAAGTGC CTCCTCCAAC 
AGTCTGAAGC GAAAGCTGAA GGAGATGCCC AAGCAGACCA GCAAGCCGCC AAAGAAAAAG 
GTGGCTAAGC AGGTACCGCT GCAACCGCAA CTTACTCCGC ATCCGCAATT CCAGCTGGCG 
CCGGCTGCCA CGGCGGCCGT CAGTATACCA GCCATTAGCA ATAACAACAA CCACCTGCCC 
CACCTGGACA CGCTGCTCTC CATGCCGAGC ACCTCGGCTC AGGCAGCAGC ACTCAATGCC 
GCCGCCGTAG CAGCCGCCAG TACGGTTCTG GATCTGGCAT CCCCTAGCCG GAAAATTGAC 
CTTAACACCA GCAACAATTT CTACAATCTG ATCACGGCCG CCTCCCTGGC AGCCAGCGGG 
AATCCCAGTC CGCATTCTGG AGACGGCCAA GCCAAGGTGA TTGTGGGAGC TCGGCCCTCT 
CCCCATGTGA TCAACCTGGA TGACTATCAA TGCACCAGCG ATATACTTCA GACGTCCAAG 
CAGCTAGCTG CCACCACCAC TGTCATCACC GCCATCTCAA AGGCCGCGCA AACGACGTCG 
GTAGCCAGGG AAAGCAGCAG CGAGTCGGAT GGCGTTGAGA TTGTGGGAGT TTTTCCTGCC 
TCTAAGCCCC AAAAGAAAGT CCAGTCTAAG CCCAAGAACA AGACTCAGAA CAGAGGTCGG 
TCGTCTTTGG GTGCGGTGGG GCAGGTAAAT GGATCCCTGG GCTACAGCGC GAATAACATG 
TATATCTACA ATAGCCCAAG AACGCTGGGT CCAGTGTACG ATCTCACCGA TCCGCATATA 
ATGAAAACTA TGTCAAATCT GAAGGAAATG GAAAAGCAAT TCATTCAAGC GGCTTTCTCG 
CCGAACAGCG TCAAGGGCGC AAGCGGAGGA ATGGGATCCT CAGCGCCCAG TACGCCCACG 
CGCCAATGAC ATTCGGGATC GGCCGCACAG CAGCCCCTTC CGGCTGCTGT GACGGCCTTT 
TACAACCAGG AGGCGATGGC CGCCGCCATC GTGCTCTCCA CGATGAGCCA CGCCTCGCCG 
CGCTACGACC TGCTGGCCAA CGGCTCCGTC TCCATGTCCG CCGCCCAGGT GGGAGCCTCT 
GCCAACACAC TGCCACAAGT ATTCGTTAAA CCCAGCCTGC CGCAGCCCAG CGCTGGCGGC 
GATGTGGTGA CCACGACTGG AGCTGCTCGC CCATCCACGC AACAACTGCC CACAAAGTTC 
ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263054.1
CDS	679..3984
Translation	MSKGGEHKRVTLTSIIQGDAGFSRFGSNILEPDAASAAPDNSSKPTTKTAKCIRIKLDLFETDSNKYPEFNYSRLLYLEKKKTKKLKQVSTTNGSASTDPFADNDDDVARIVKELEAKYGNSYATGRGKSKKDDYRDIGMGYDESDSFIDNTEAYDEIIPEEAETLEGGFYINCGALEFKNLTKKSYTTRTDAIIKMPERSRKRMVSSSSESSSSSSGDDDENDDGNNEEDDESDSEDDSEENDESDSEDDSESESLEDEDSAATAKSSSKYKDNHQAKRAKVIVTGKSKPSSSSLTSGKKPPTKPITTSSSSNSPRPSTVEISDTEDGQDPIQTQPSSQLQSLPQSQAQAQALKKVVKTTTVKDMLKAKRDSFLKSQSGTAAVKGVGNGELKCVSTDVSSSDSSDMESEHGRADRQAGQHGKDGQENLRTADTLLPTTLDADIVTAVNSFKEAVKSRDMCGKKFNLDVKLSPLLLRVYEAVLCTDRNERNMVFSHIEYQLQLPKYYMLRKGKQVRAKEEKRKSTIMLEKLRRAVAVVMPKAVANYETELRTFAEQAAADVNSELPPKMPRKKFQWTSELRHLLYDVYQARWTSYAFLAKRKESLEEFINWYLKEKVVELWPPGWMRLDELQREITRYKNAKLKAKEKPKAPPASASPKPVGVVSAPEQMPPASSYLKAVEDPRSRGNSDTDSATSASSNSLKRKLKEMPKQTSKPPKKKVAKQVPLQPQLTPHPQFQLAPAATAAVSIPAISNNNNHLPHLDTLLSMPSTSAQAAALNAAAVAAASTVLDLASPSRKIDLNTSNNFYNLITAASLAASGNPSPHSGDGQAKVIVGARPSPHVINLDDYQCTSDILQTSKQLAATTTVITAISKAAQTTSVARESSSESDGVEIVGVFPASKPQKKVQSKPKNKTQNRGRSSLGAVGQVNGSLGYSANNMYIYNSPRTLGPVYDLTDPHIMKTMSNLKEMEKQFIQAAFSPNSVKGASGGMGSSAPSTPTRQ*HSGSAAQQPLPAAVTAFYNQEAMAAAIVLSTMSHASPRYDLLANGSVSMSAAQVGASANTLPQVFVKPSLPQPSAGGDVVTTTGAARPSTQQLPTKFI

Target ORF information:

RefSeq Version	NM_001276125.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster yemanuclein (yem), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276125.1

ORF Insert Sequence:

ATGTCAAAGG GGGGCGAGCA CAAGCGCGTC ACCCTCACCT CGATCATCCA GGGCGATGCG 
GGCTTCTCGC GCTTCGGCAG CAACATCCTG GAGCCTGATG CTGCGTCCGC TGCACCCGAC 
AACAGCTCCA AACCCACGAC AAAGACTGCC AAGTGCATCC GCATCAAACT CGATCTGTTC 
GAGACCGATT CGAACAAGTA TCCCGAGTTT AACTACTCCC GGCTTTTGTA TTTGGAAAAG 
AAAAAGACCA AAAAGCTGAA GCAGGTGAGC ACTACCAATG GCTCCGCAAG CACTGATCCC 
TTTGCCGACA ACGATGACGA TGTGGCACGG ATCGTCAAGG AGCTGGAGGC CAAGTATGGC 
AACTCCTATG CCACAGGCAG GGGGAAGAGC AAAAAGGACG ACTACCGGGA CATTGGAATG 
GGCTACGATG AGTCGGACTC ATTCATCGAC AACACGGAAG CCTACGACGA AATCATACCG 
GAGGAGGCGG AAACTCTGGA GGGCGGCTTC TACATCAACT GTGGCGCACT GGAGTTCAAG 
AACCTCACCA AGAAGTCCTA CACCACTCGA ACAGATGCCA TCATCAAAAT GCCAGAGCGA 
TCACGCAAGC GCATGGTCTC GTCCAGCTCG GAAAGCTCAT CGTCATCCTC GGGCGATGAT 
GACGAAAACG ATGATGGCAA CAATGAGGAG GACGACGAGA GCGATTCCGA GGACGACTCC 
GAGGAGAACG ATGAAAGCGA CTCCGAAGAC GACTCCGAGA GTGAAAGTCT TGAGGACGAG 
GACTCTGCTG CTACAGCCAA ATCCAGCAGC AAGTACAAAG ACAATCATCA AGCTAAGCGG 
GCCAAGGTCA TCGTTACGGG CAAGTCCAAA CCAAGCTCAA GTTCTTTGAC GAGTGGCAAG 
AAGCCGCCGA CTAAGCCGAT CACCACTTCC TCATCCTCAA ACTCGCCACG ACCCAGCACG 
GTGGAGATTT CGGACACGGA AGACGGCCAA GACCCTATCC AGACTCAGCC ATCAAGTCAG 
CTTCAGTCCC TGCCGCAGAG TCAGGCCCAG GCGCAAGCTT TAAAGAAGGT GGTTAAGACC 
ACAACGGTCA AGGACATGCT AAAGGCCAAG CGAGACAGCT TCCTGAAGTC GCAGAGCGGC 
ACGGCAGCTG TCAAGGGCGT CGGCAACGGC GAGCTGAAGT GTGTTTCCAC CGACGTGTCC 
AGCAGCGATA GCAGCGATAT GGAGTCTGAG CACGGCCGGG CAGATAGGCA GGCCGGTCAG 
CATGGCAAGG ATGGGCAGGA AAATCTTCGC ACCGCCGACA CTCTGCTCCC CACCACATTG 
GATGCGGACA TTGTGACCGC AGTCAACAGC TTCAAAGAAG CGGTGAAGAG TCGAGACATG 
TGCGGCAAGA AGTTTAATCT GGATGTGAAG CTTTCGCCAC TACTGCTTCG GGTGTACGAG 
GCGGTGCTGT GCACGGACCG CAACGAGCGC AACATGGTCT TCTCCCACAT CGAGTATCAG 
CTGCAACTGC CCAAGTACTA CATGCTGCGC AAAGGGAAAC AAGTGCGGGC GAAGGAGGAG 
AAGAGGAAGT CCACCATTAT GCTGGAGAAG CTGCGGCGGG CTGTAGCTGT GGTCATGCCT 
AAGGCGGTGG CCAACTATGA GACGGAACTG CGCACATTTG CTGAACAGGC AGCTGCGGAC 
GTGAACTCCG AACTGCCGCC CAAAATGCCG CGTAAGAAGT TCCAGTGGAC CAGCGAGCTA 
CGCCATCTGC TGTACGATGT GTACCAAGCG CGTTGGACGT CCTACGCTTT CCTAGCCAAG 
CGCAAGGAAT CGTTGGAGGA GTTCATCAAT TGGTATCTCA AGGAAAAGGT GGTGGAACTG 
TGGCCGCCAG GCTGGATGCG TTTGGATGAG TTGCAAAGGG AAATAACACG CTACAAGAAC 
GCCAAGCTGA AAGCAAAGGA GAAGCCAAAG GCACCGCCGG CGTCCGCATC GCCCAAGCCC 
GTTGGAGTTG TATCTGCTCC TGAGCAAATG CCGCCGGCAA GCAGCTACCT AAAGGCAGTG 
GAGGACCCTC GATCTCGAGG TAACTCCGAT ACGGACTCAG CTACAAGTGC CTCCTCCAAC 
AGTCTGAAGC GAAAGCTGAA GGAGATGCCC AAGCAGACCA GCAAGCCGCC AAAGAAAAAG 
GTGGCTAAGC AGGTACCGCT GCAACCGCAA CTTACTCCGC ATCCGCAATT CCAGCTGGCG 
CCGGCTGCCA CGGCGGCCGT CAGTATACCA GCCATTAGCA ATAACAACAA CCACCTGCCC 
CACCTGGACA CGCTGCTCTC CATGCCGAGC ACCTCGGCTC AGGCAGCAGC ACTCAATGCC 
GCCGCCGTAG CAGCCGCCAG TACGGTTCTG GATCTGGCAT CCCCTAGCCG GAAAATTGAC 
CTTAACACCA GCAACAATTT CTACAATCTG ATCACGGCCG CCTCCCTGGC AGCCAGCGGG 
AATCCCAGTC CGCATTCTGG AGACGGCCAA GCCAAGGTGA TTGTGGGAGC TCGGCCCTCT 
CCCCATGTGA TCAACCTGGA TGACTATCAA TGCACCAGCG ATATACTTCA GACGTCCAAG 
CAGCTAGCTG CCACCACCAC TGTCATCACC GCCATCTCAA AGGCCGCGCA AACGACGTCG 
GTAGCCAGGG AAAGCAGCAG CGAGTCGGAT GGCGTTGAGA TTGTGGGAGT TTTTCCTGCC 
TCTAAGCCCC AAAAGAAAGT CCAGTCTAAG CCCAAGAACA AGACTCAGAA CAGAGGTCGG 
TCGTCTTTGG GTGCGGTGGG GCAGGTAAAT GGATCCCTGG GCTACAGCGC GAATAACATG 
TATATCTACA ATAGCCCAAG AACGCTGGGT CCAGTGTACG ATCTCACCGA TCCGCATATA 
ATGAAAACTA TGTCAAATCT GAAGGAAATG GAAAAGCAAT TCATTCAAGC GGCTTTCTCG 
CCGAACAGCG TCAAGGGCGC AAGCGGAGGA ATGGGATCCT CAGCGCCCAG TACGCCCACG 
CGCCAATGAC ATTCGGGATC GGCCGCACAG CAGCCCCTTC CGGCTGCTGT GACGGCCTTT 
TACAACCAGG AGGCGATGGC CGCCGCCATC GTGCTCTCCA CGATGAGCCA CGCCTCGCCG 
CGCTACGACC TGCTGGCCAA CGGCTCCGTC TCCATGTCCG CCGCCCAGGT GGGAGCCTCT 
GCCAACACAC TGCCACAAGT ATTCGTTAAA CCCAGCCTGC CGCAGCCCAG CGCTGGCGGC 
GATGTGGTGA CCACGACTGG AGCTGCTCGC CCATCCACGC AACAACTGCC CACAAAGTTC 
ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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yem cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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