Pdhb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdhb
Entrez Gene ID	43437
Full Name	CG11876 gene product from transcript CG11876-RA
Synonyms	CG 11876,Dmel\CG11876,E1beta,PDHB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F10-98F10

mRNA and Protein(s)

mRNA	Protein	Name
NM_170388.2	NP_733267.1	pyruvate dehydrogenase E1 beta subunit, isoform C
NM_143411.4	NP_651668.1	pyruvate dehydrogenase E1 beta subunit, isoform D
NM_170387.2	NP_733266.1	pyruvate dehydrogenase E1 beta subunit, isoform B
NM_170386.2	NP_733265.1	pyruvate dehydrogenase E1 beta subunit, isoform A

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	pdb1	NP_596272.1
Bos taurus (cattle)	PDHB	NP_001030512.2
Danio rerio (zebrafish)	pdhb	NP_998319.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010421	XP_311527.2
Neurospora crassa	NCU03004	XP_965390.1
Xenopus tropicalis (tropical clawed frog)	pdhb	NP_001016212.1
Mus musculus (house mouse)	Pdhb	NP_077183.1
Rattus norvegicus (Norway rat)	Pdhb	NP_001007621.1
Gallus gallus (chicken)	PDHB	NP_001185549.1
Caenorhabditis elegans (roundworm)	pdhb-1	NP_500340.1
Saccharomyces cerevisiae (baker's yeast)	PDB1	NP_009780.1
Kluyveromyces lactis	KLLA0F09603g	XP_455516.1
Arabidopsis thaliana (thale cress)	MAB1	NP_199898.1
Oryza sativa (rice)	Os08g0536000	NP_001062360.1
Homo sapiens (human)	PDHB	NP_000916.2
Pan troglodytes (chimpanzee)	PDHB	NP_001233347.1
	PDHB	XP_001096445.1
Canis lupus familiaris (dog)	PDHB	XP_533778.2
Magnaporthe oryzae (rice blast fungus)	MGG_10569	XP_003721383.1
Drosophila melanogaster (fruit fly)	CG11876	NP_651668.1
Eremothecium gossypii	AGOS_AAR167C	NP_982710.1
Oryza sativa (rice)	Os09g0509200	NP_001063627.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pdhb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdhb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06457	NM_170386.2 Latest version!	Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant A (Pdhb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa06457	NM_143411.4 Latest version!	Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant D (Pdhb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa06458	NM_170387.2 Latest version!	Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant B (Pdhb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06458	NM_170388.2 Latest version!	Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant C (Pdhb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdhb ( NM_143411.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651668.1 Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant D (Pdhb), mRNA.

Price & Availability↑

CloneID	OFa06457
Clone ID Related Accession (Same CDS sequence)	NM_143411.4 , NM_170386.2
Accession Version	NM_143411.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase E1 beta subunit, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143411.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCCGAA CCCGACTCAT CCAGGCGGCC AGCTCCGCCC AGCGAGCGTT CTCCACCAGC 
CAGAAGGCCC TGGCGGCCAA GCAGATGACC GTGCGAGATG CCCTCAACAG CGCATTGGAC 
GATGAGCTGG CCCGCGACGA CCGCGTCTTC ATCCTGGGCG AGGAGGTGGC CCAGTACGAC 
GGTGCTTACA AGGTTTCCCG TGGACTGTGG AAGAAGTACG GCGACAAGCG CGTCATCGAC 
ACGCCAATCA CAGAGATGGG CTTTGCCGGC ATCGCTGTTG GCGCTGCCAT GGCCGGTCTG 
CGTCCCGTGT GCGAGTTCAT GACCTGGAAC TTCTCCATGC AGGCCATCGA TCACATCATC 
AACTCGGCGG CCAAGACCTT CTACATGTCT GCCGGTGCCG TCAATGTGCC CATTGTGTTC 
CGTGGTCCCA ATGGAGCCGC TTCCGGTGTT GCCGCCCAGC ACTCTCAGTG CTTCGCCGCC 
TGGTACGCCC ACTGCCCCGG CCTGAAGGTC CTTTCGCCAT ACGATGCCGA GGATGCCCGC 
GGACTGTTGA AGTCCGCAAT CCGTGATCCC GATCCAGTGG TCTTCCTGGA GAATGAGCTG 
GTCTATGGCA CGGCATTCCC TGTGGCCGAT AACGTGGCCG ACAAGGACTT CTTGGTGCCC 
ATCGGCAAGG CCAAGGTAAT GCGGCCGGGC AAGGACATTA CCCTGGTGGC GCATTCCAAG 
GCGGTGGAGA CATCGCTTCT GGCAGCTGCC GAGCTGGCCA AGAAGGGTAT TGAGGCTGAG 
GTCATTAACC TGCGCTCCAT TCGTCCTCTG GACACGGCGA CCATCTTCGC CTCTGTACGA 
AAGACCCACC ATCTGGTGAC CGTGGAGAAC GGCTGGCCCC AACACGGCGT TGGAGCTGAG 
ATCTGCGCCC GCATAATGGA GGACCAGACC TTCTTCGAGC TGGACGCCCC CGTATGGCGC 
TGCGCCGGCG TGGATGTGCC CATGCCCTAT GCCAAGACGC TGGAGGCCCA CGCCCTGCCC 
CGCGTTCAGG ATCTCGTGGA GGCGACTCTT AAGGTCTTGG GTGGCAAAGT GGGCAAGGCC 
GCTGCGGCCA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651668.1
CDS	51..1148
Translation	MLRTRLIQAASSAQRAFSTSQKALAAKQMTVRDALNSALDDELARDDRVFILGEEVAQYDGAYKVSRGLWKKYGDKRVIDTPITEMGFAGIAVGAAMAGLRPVCEFMTWNFSMQAIDHIINSAAKTFYMSAGAVNVPIVFRGPNGAASGVAAQHSQCFAAWYAHCPGLKVLSPYDAEDARGLLKSAIRDPDPVVFLENELVYGTAFPVADNVADKDFLVPIGKAKVMRPGKDITLVAHSKAVETSLLAAAELAKKGIEAEVINLRSIRPLDTATIFASVRKTHHLVTVENGWPQHGVGAEICARIMEDQTFFELDAPVWRCAGVDVPMPYAKTLEAHALPRVQDLVEATLKVLGGKVGKAAAANK

Target ORF information:

RefSeq Version	NM_143411.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant D (Pdhb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143411.4

ORF Insert Sequence:

ATGCTCCGAA CCCGACTCAT CCAGGCGGCC AGCTCCGCCC AGCGAGCGTT CTCCACCAGC 
CAGAAGGCCC TGGCGGCCAA GCAGATGACC GTGCGAGATG CCCTCAACAG CGCATTGGAC 
GATGAGCTGG CCCGCGACGA CCGCGTCTTC ATCCTGGGCG AGGAGGTGGC CCAGTACGAC 
GGTGCTTACA AGGTTTCCCG TGGACTGTGG AAGAAGTACG GCGACAAGCG CGTCATCGAC 
ACGCCAATCA CAGAGATGGG CTTTGCCGGC ATCGCTGTTG GCGCTGCCAT GGCCGGTCTG 
CGTCCCGTGT GCGAGTTCAT GACCTGGAAC TTCTCCATGC AGGCCATCGA TCACATCATC 
AACTCGGCGG CCAAGACCTT CTACATGTCT GCCGGTGCCG TCAATGTGCC CATTGTGTTC 
CGTGGTCCCA ATGGAGCCGC TTCCGGTGTT GCCGCCCAGC ACTCTCAGTG CTTCGCCGCC 
TGGTACGCCC ACTGCCCCGG CCTGAAGGTC CTTTCGCCAT ACGATGCCGA GGATGCCCGC 
GGACTGTTGA AGTCCGCAAT CCGTGATCCC GATCCAGTGG TCTTCCTGGA GAATGAGCTG 
GTCTATGGCA CGGCATTCCC TGTGGCCGAT AACGTGGCCG ACAAGGACTT CTTGGTGCCC 
ATCGGCAAGG CCAAGGTAAT GCGGCCGGGC AAGGACATTA CCCTGGTGGC GCATTCCAAG 
GCGGTGGAGA CATCGCTTCT GGCAGCTGCC GAGCTGGCCA AGAAGGGTAT TGAGGCTGAG 
GTCATTAACC TGCGCTCCAT TCGTCCTCTG GACACGGCGA CCATCTTCGC CTCTGTACGA 
AAGACCCACC ATCTGGTGAC CGTGGAGAAC GGCTGGCCCC AACACGGCGT TGGAGCTGAG 
ATCTGCGCCC GCATAATGGA GGACCAGACC TTCTTCGAGC TGGACGCCCC CGTATGGCGC 
TGCGCCGGCG TGGATGTGCC CATGCCCTAT GCCAAGACGC TGGAGGCCCA CGCCCTGCCC 
CGCGTTCAGG ATCTCGTGGA GGCGACTCTT AAGGTCTTGG GTGGCAAAGT GGGCAAGGCC 
GCTGCGGCCA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdhb ( NM_170386.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733265.1 Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant A (Pdhb), mRNA.

Price & Availability↑

CloneID	OFa06457
Clone ID Related Accession (Same CDS sequence)	NM_143411.4 , NM_170386.2
Accession Version	NM_170386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase E1 beta subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_170386.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCCGAA CCCGACTCAT CCAGGCGGCC AGCTCCGCCC AGCGAGCGTT CTCCACCAGC 
CAGAAGGCCC TGGCGGCCAA GCAGATGACC GTGCGAGATG CCCTCAACAG CGCATTGGAC 
GATGAGCTGG CCCGCGACGA CCGCGTCTTC ATCCTGGGCG AGGAGGTGGC CCAGTACGAC 
GGTGCTTACA AGGTTTCCCG TGGACTGTGG AAGAAGTACG GCGACAAGCG CGTCATCGAC 
ACGCCAATCA CAGAGATGGG CTTTGCCGGC ATCGCTGTTG GCGCTGCCAT GGCCGGTCTG 
CGTCCCGTGT GCGAGTTCAT GACCTGGAAC TTCTCCATGC AGGCCATCGA TCACATCATC 
AACTCGGCGG CCAAGACCTT CTACATGTCT GCCGGTGCCG TCAATGTGCC CATTGTGTTC 
CGTGGTCCCA ATGGAGCCGC TTCCGGTGTT GCCGCCCAGC ACTCTCAGTG CTTCGCCGCC 
TGGTACGCCC ACTGCCCCGG CCTGAAGGTC CTTTCGCCAT ACGATGCCGA GGATGCCCGC 
GGACTGTTGA AGTCCGCAAT CCGTGATCCC GATCCAGTGG TCTTCCTGGA GAATGAGCTG 
GTCTATGGCA CGGCATTCCC TGTGGCCGAT AACGTGGCCG ACAAGGACTT CTTGGTGCCC 
ATCGGCAAGG CCAAGGTAAT GCGGCCGGGC AAGGACATTA CCCTGGTGGC GCATTCCAAG 
GCGGTGGAGA CATCGCTTCT GGCAGCTGCC GAGCTGGCCA AGAAGGGTAT TGAGGCTGAG 
GTCATTAACC TGCGCTCCAT TCGTCCTCTG GACACGGCGA CCATCTTCGC CTCTGTACGA 
AAGACCCACC ATCTGGTGAC CGTGGAGAAC GGCTGGCCCC AACACGGCGT TGGAGCTGAG 
ATCTGCGCCC GCATAATGGA GGACCAGACC TTCTTCGAGC TGGACGCCCC CGTATGGCGC 
TGCGCCGGCG TGGATGTGCC CATGCCCTAT GCCAAGACGC TGGAGGCCCA CGCCCTGCCC 
CGCGTTCAGG ATCTCGTGGA GGCGACTCTT AAGGTCTTGG GTGGCAAAGT GGGCAAGGCC 
GCTGCGGCCA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733265.1
CDS	120..1217
Translation	MLRTRLIQAASSAQRAFSTSQKALAAKQMTVRDALNSALDDELARDDRVFILGEEVAQYDGAYKVSRGLWKKYGDKRVIDTPITEMGFAGIAVGAAMAGLRPVCEFMTWNFSMQAIDHIINSAAKTFYMSAGAVNVPIVFRGPNGAASGVAAQHSQCFAAWYAHCPGLKVLSPYDAEDARGLLKSAIRDPDPVVFLENELVYGTAFPVADNVADKDFLVPIGKAKVMRPGKDITLVAHSKAVETSLLAAAELAKKGIEAEVINLRSIRPLDTATIFASVRKTHHLVTVENGWPQHGVGAEICARIMEDQTFFELDAPVWRCAGVDVPMPYAKTLEAHALPRVQDLVEATLKVLGGKVGKAAAANK

Target ORF information:

RefSeq Version	NM_170386.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant A (Pdhb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170386.2

ORF Insert Sequence:

ATGCTCCGAA CCCGACTCAT CCAGGCGGCC AGCTCCGCCC AGCGAGCGTT CTCCACCAGC 
CAGAAGGCCC TGGCGGCCAA GCAGATGACC GTGCGAGATG CCCTCAACAG CGCATTGGAC 
GATGAGCTGG CCCGCGACGA CCGCGTCTTC ATCCTGGGCG AGGAGGTGGC CCAGTACGAC 
GGTGCTTACA AGGTTTCCCG TGGACTGTGG AAGAAGTACG GCGACAAGCG CGTCATCGAC 
ACGCCAATCA CAGAGATGGG CTTTGCCGGC ATCGCTGTTG GCGCTGCCAT GGCCGGTCTG 
CGTCCCGTGT GCGAGTTCAT GACCTGGAAC TTCTCCATGC AGGCCATCGA TCACATCATC 
AACTCGGCGG CCAAGACCTT CTACATGTCT GCCGGTGCCG TCAATGTGCC CATTGTGTTC 
CGTGGTCCCA ATGGAGCCGC TTCCGGTGTT GCCGCCCAGC ACTCTCAGTG CTTCGCCGCC 
TGGTACGCCC ACTGCCCCGG CCTGAAGGTC CTTTCGCCAT ACGATGCCGA GGATGCCCGC 
GGACTGTTGA AGTCCGCAAT CCGTGATCCC GATCCAGTGG TCTTCCTGGA GAATGAGCTG 
GTCTATGGCA CGGCATTCCC TGTGGCCGAT AACGTGGCCG ACAAGGACTT CTTGGTGCCC 
ATCGGCAAGG CCAAGGTAAT GCGGCCGGGC AAGGACATTA CCCTGGTGGC GCATTCCAAG 
GCGGTGGAGA CATCGCTTCT GGCAGCTGCC GAGCTGGCCA AGAAGGGTAT TGAGGCTGAG 
GTCATTAACC TGCGCTCCAT TCGTCCTCTG GACACGGCGA CCATCTTCGC CTCTGTACGA 
AAGACCCACC ATCTGGTGAC CGTGGAGAAC GGCTGGCCCC AACACGGCGT TGGAGCTGAG 
ATCTGCGCCC GCATAATGGA GGACCAGACC TTCTTCGAGC TGGACGCCCC CGTATGGCGC 
TGCGCCGGCG TGGATGTGCC CATGCCCTAT GCCAAGACGC TGGAGGCCCA CGCCCTGCCC 
CGCGTTCAGG ATCTCGTGGA GGCGACTCTT AAGGTCTTGG GTGGCAAAGT GGGCAAGGCC 
GCTGCGGCCA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdhb ( NM_170388.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733267.1 Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant C (Pdhb), mRNA.

Price & Availability↑

CloneID	OFa06458
Clone ID Related Accession (Same CDS sequence)	NM_170388.2 , NM_170387.2
Accession Version	NM_170388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase E1 beta subunit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_170388.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCCGAA CCCGACTCAT CCAGGCGGCC AGCTCCGCCC AGCGAGCGTT CTCCACCAGC 
CAGAAGGCCC TGGCGGCCAA GCAGATGACC GTGCGAGATG CCCTCAACAG CGCATTGGAC 
GATGAGCTGG CCCGCGACGA CCGCGTCTTC ATCCTGGGCG AGGAGGTGGC CCAGTACGAC 
GGTGCTTACA AGGTTTCCCG TGGACTGTGG AAGAAGTACG GCGACAAGCG CGTCATCGAC 
ACGCCAATCA CAGAGATGGG CTTTGCCGGC ATCGCTGTTG GCGCTGCCAT GGCCGGTCTG 
CGTCCCGTGT GCGAGTTCAT GACCTGGAAC TTCTCCATGC AGGCCATCGA TCACGCAAAG 
ATACTCGATT GCGCCAAGCC GCCAGTCGGT GATCGCCCTC TGCCAATAAG CCTCATAATT 
GAAAGTCCAC GCTCAACTTG GCTGAATGAG ATCAACGACA TCCTGCACAG AGATCGAGTC 
GGCGAGGTGA TTGCGCCGCC GCTGGACAAG AGCAAGTCGG ACAAGATGGA GGCGGCCCGC 
CTCATCGTCA TTCGGCGGCG CAAGATGAAG AAGCACAAGC TAAAGAAGCT GCGTCGCAAG 
ATGAAGTTCG AGTGGGCCAA GGTGCGCCAG CGCCGCGAGA TGCGCAAAGA GAAGGCCTTC 
CAAGCAAAGC TGATATCCCA GATCAAGCAG GCGGAGGCAT TCAGTGCGGA GCAGCACGTG 
GCGGAGATCC TGCGGCAGGC CAACGAGACG CCGCTGCCGC GTTTCTGGAA GGGACGCCGC 
CTGCCGGCAT TTATCATTAA GCAGAAACTA GGCATTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733267.1
CDS	120..941
Translation	MLRTRLIQAASSAQRAFSTSQKALAAKQMTVRDALNSALDDELARDDRVFILGEEVAQYDGAYKVSRGLWKKYGDKRVIDTPITEMGFAGIAVGAAMAGLRPVCEFMTWNFSMQAIDHAKILDCAKPPVGDRPLPISLIIESPRSTWLNEINDILHRDRVGEVIAPPLDKSKSDKMEAARLIVIRRRKMKKHKLKKLRRKMKFEWAKVRQRREMRKEKAFQAKLISQIKQAEAFSAEQHVAEILRQANETPLPRFWKGRRLPAFIIKQKLGIK

Target ORF information:

RefSeq Version	NM_170388.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant C (Pdhb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170388.2

ORF Insert Sequence:

ATGCTCCGAA CCCGACTCAT CCAGGCGGCC AGCTCCGCCC AGCGAGCGTT CTCCACCAGC 
CAGAAGGCCC TGGCGGCCAA GCAGATGACC GTGCGAGATG CCCTCAACAG CGCATTGGAC 
GATGAGCTGG CCCGCGACGA CCGCGTCTTC ATCCTGGGCG AGGAGGTGGC CCAGTACGAC 
GGTGCTTACA AGGTTTCCCG TGGACTGTGG AAGAAGTACG GCGACAAGCG CGTCATCGAC 
ACGCCAATCA CAGAGATGGG CTTTGCCGGC ATCGCTGTTG GCGCTGCCAT GGCCGGTCTG 
CGTCCCGTGT GCGAGTTCAT GACCTGGAAC TTCTCCATGC AGGCCATCGA TCACGCAAAG 
ATACTCGATT GCGCCAAGCC GCCAGTCGGT GATCGCCCTC TGCCAATAAG CCTCATAATT 
GAAAGTCCAC GCTCAACTTG GCTGAATGAG ATCAACGACA TCCTGCACAG AGATCGAGTC 
GGCGAGGTGA TTGCGCCGCC GCTGGACAAG AGCAAGTCGG ACAAGATGGA GGCGGCCCGC 
CTCATCGTCA TTCGGCGGCG CAAGATGAAG AAGCACAAGC TAAAGAAGCT GCGTCGCAAG 
ATGAAGTTCG AGTGGGCCAA GGTGCGCCAG CGCCGCGAGA TGCGCAAAGA GAAGGCCTTC 
CAAGCAAAGC TGATATCCCA GATCAAGCAG GCGGAGGCAT TCAGTGCGGA GCAGCACGTG 
GCGGAGATCC TGCGGCAGGC CAACGAGACG CCGCTGCCGC GTTTCTGGAA GGGACGCCGC 
CTGCCGGCAT TTATCATTAA GCAGAAACTA GGCATTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdhb ( NM_170387.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733266.1 Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant B (Pdhb), mRNA.

Price & Availability↑

CloneID	OFa06458
Clone ID Related Accession (Same CDS sequence)	NM_170388.2 , NM_170387.2
Accession Version	NM_170387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase E1 beta subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_170387.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCCGAA CCCGACTCAT CCAGGCGGCC AGCTCCGCCC AGCGAGCGTT CTCCACCAGC 
CAGAAGGCCC TGGCGGCCAA GCAGATGACC GTGCGAGATG CCCTCAACAG CGCATTGGAC 
GATGAGCTGG CCCGCGACGA CCGCGTCTTC ATCCTGGGCG AGGAGGTGGC CCAGTACGAC 
GGTGCTTACA AGGTTTCCCG TGGACTGTGG AAGAAGTACG GCGACAAGCG CGTCATCGAC 
ACGCCAATCA CAGAGATGGG CTTTGCCGGC ATCGCTGTTG GCGCTGCCAT GGCCGGTCTG 
CGTCCCGTGT GCGAGTTCAT GACCTGGAAC TTCTCCATGC AGGCCATCGA TCACGCAAAG 
ATACTCGATT GCGCCAAGCC GCCAGTCGGT GATCGCCCTC TGCCAATAAG CCTCATAATT 
GAAAGTCCAC GCTCAACTTG GCTGAATGAG ATCAACGACA TCCTGCACAG AGATCGAGTC 
GGCGAGGTGA TTGCGCCGCC GCTGGACAAG AGCAAGTCGG ACAAGATGGA GGCGGCCCGC 
CTCATCGTCA TTCGGCGGCG CAAGATGAAG AAGCACAAGC TAAAGAAGCT GCGTCGCAAG 
ATGAAGTTCG AGTGGGCCAA GGTGCGCCAG CGCCGCGAGA TGCGCAAAGA GAAGGCCTTC 
CAAGCAAAGC TGATATCCCA GATCAAGCAG GCGGAGGCAT TCAGTGCGGA GCAGCACGTG 
GCGGAGATCC TGCGGCAGGC CAACGAGACG CCGCTGCCGC GTTTCTGGAA GGGACGCCGC 
CTGCCGGCAT TTATCATTAA GCAGAAACTA GGCATTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733266.1
CDS	154..975
Translation	MLRTRLIQAASSAQRAFSTSQKALAAKQMTVRDALNSALDDELARDDRVFILGEEVAQYDGAYKVSRGLWKKYGDKRVIDTPITEMGFAGIAVGAAMAGLRPVCEFMTWNFSMQAIDHAKILDCAKPPVGDRPLPISLIIESPRSTWLNEINDILHRDRVGEVIAPPLDKSKSDKMEAARLIVIRRRKMKKHKLKKLRRKMKFEWAKVRQRREMRKEKAFQAKLISQIKQAEAFSAEQHVAEILRQANETPLPRFWKGRRLPAFIIKQKLGIK

Target ORF information:

RefSeq Version	NM_170387.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase E1 beta subunit, transcript variant B (Pdhb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170387.2

ORF Insert Sequence:

ATGCTCCGAA CCCGACTCAT CCAGGCGGCC AGCTCCGCCC AGCGAGCGTT CTCCACCAGC 
CAGAAGGCCC TGGCGGCCAA GCAGATGACC GTGCGAGATG CCCTCAACAG CGCATTGGAC 
GATGAGCTGG CCCGCGACGA CCGCGTCTTC ATCCTGGGCG AGGAGGTGGC CCAGTACGAC 
GGTGCTTACA AGGTTTCCCG TGGACTGTGG AAGAAGTACG GCGACAAGCG CGTCATCGAC 
ACGCCAATCA CAGAGATGGG CTTTGCCGGC ATCGCTGTTG GCGCTGCCAT GGCCGGTCTG 
CGTCCCGTGT GCGAGTTCAT GACCTGGAAC TTCTCCATGC AGGCCATCGA TCACGCAAAG 
ATACTCGATT GCGCCAAGCC GCCAGTCGGT GATCGCCCTC TGCCAATAAG CCTCATAATT 
GAAAGTCCAC GCTCAACTTG GCTGAATGAG ATCAACGACA TCCTGCACAG AGATCGAGTC 
GGCGAGGTGA TTGCGCCGCC GCTGGACAAG AGCAAGTCGG ACAAGATGGA GGCGGCCCGC 
CTCATCGTCA TTCGGCGGCG CAAGATGAAG AAGCACAAGC TAAAGAAGCT GCGTCGCAAG 
ATGAAGTTCG AGTGGGCCAA GGTGCGCCAG CGCCGCGAGA TGCGCAAAGA GAAGGCCTTC 
CAAGCAAAGC TGATATCCCA GATCAAGCAG GCGGAGGCAT TCAGTGCGGA GCAGCACGTG 
GCGGAGATCC TGCGGCAGGC CAACGAGACG CCGCTGCCGC GTTTCTGGAA GGGACGCCGC 
CTGCCGGCAT TTATCATTAA GCAGAAACTA GGCATTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pdhb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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