Cpsf100 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpsf100
Entrez Gene ID	43426
Full Name	Cleavage and polyadenylation specificity factor 100
Synonyms	BcDNA:LD14168,CG1957,CPSF-100,CPSF100,Dmel\CG1957
General protein information	Preferred Names Cleavage and polyadenylation specificity factor 100 Names CG1957 gene product from transcript CG1957-RA CG1957-PA Cpsf100-PA cleavage and polyadenylation specificity factor 100
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F5-98F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_143401.3	NP_651658.1	cleavage and polyadenylation specificity factor 100

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CPSF2	XP_537353.2
Drosophila melanogaster (fruit fly)	Cpsf100	NP_651658.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002474	XP_312464.4
Caenorhabditis elegans (roundworm)	cpsf-2	NP_504822.1
Arabidopsis thaliana (thale cress)	CPSF100	NP_197776.1
Mus musculus (house mouse)	Cpsf2	NP_058552.1
Danio rerio (zebrafish)	cpsf2	NP_001002384.1
Bos taurus (cattle)	CPSF2	NP_787002.1
Homo sapiens (human)	CPSF2	NP_059133.1
Pan troglodytes (chimpanzee)	CPSF2	XP_001147277.1
Oryza sativa (rice)	Os09g0569400	NP_001063978.1
Xenopus tropicalis (tropical clawed frog)	cpsf2	NP_001120452.1
	CPSF2	XP_001092942.1
Rattus norvegicus (Norway rat)	Cpsf2	NP_001100223.1
Gallus gallus (chicken)	CPSF2	NP_001026379.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors propose a model in which the core cleavage complex formation is mediated by CPSF73, CPSF100, and Symplekin C-termini, and the N-terminal region of Symplekin facilitates cotranscriptional 3' end processing of histone mRNAs.
Title: In vivo characterization of the Drosophila mRNA 3' end processing core cleavage complex.

Data demonstrate that Symplekin (CG2097), CPSF73 (CG7698), and CPSF100 (CG1957) are present in a stable complex and interact with histone-specific processing factors.
Title: A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs.

The following Cpsf100 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpsf100 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06445	NM_143401.3 Latest version!	Drosophila melanogaster cleavage and polyadenylation specificity factor 100 (Cpsf100), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpsf100 ( NM_143401.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651658.1 Drosophila melanogaster cleavage and polyadenylation specificity factor 100 (Cpsf100), mRNA.

Price & Availability↑

CloneID	OFa06445
Clone ID Related Accession (Same CDS sequence)	NM_143401.3
Accession Version	NM_143401.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cleavage and polyadenylation specificity factor 100
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143401.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCCA TTATCAAGCT GCACACTATA TCTGGGGCGA TGGACGAGTC CCCGCCATGC 
TATATCCTAC AGATAGACGA TGTGCGGATA TTACTTGACT GCGGATGGGA CGAAAAGTTC 
GATGCCAACT TTATCAAGGA ACTGAAGAGG CAGGTGCACA CCCTGGACGC CGTGCTGCTA 
TCCCATCCGG ATGCATATCA TCTTGGAGCC CTGCCATACT TGGTGGGCAA GCTGGGGCTC 
AATTGCCCCA TCTACGCCAC CATTCCCGTG TTCAAAATGG GTCAAATGTT TATGTATGAC 
CTTTACATGT CGCACTTCAA TATGGGAGAC TTCGATCTAT TTTCGCTGGA CGACGTGGAC 
ACGGCTTTCG AAAAGATAAC CCAGTTGAAA TACAACCAAA CAGTTTCACT GAAGGACAAA 
GGATATGGAA TATCCATTAC GCCTTTGAAC GCTGGACACA TGATCGGCGG CACCATCTGG 
AAGATCGTCA AGGTGGGCGA GGAGGATATT GTGTACGCCA CCGACTTCAA CCATAAGAAG 
GAGCGCCACT TAAGTGGCTG CGAGCTGGAC AGGCTGCAAA GACCTTCGCT GCTCATTACC 
GACGCCTACA ACGCACAGTA CCAGCAGGCC AGAAGGAGGG CCCGCGATGA AAAGCTAATG 
ACCAACATCC TGCAAACGGT GCGTAACAAC GGCAATGTCC TGATAGCCGT GGATACGGCA 
GGACGAGTCC TGGAGTTGGC CCACATGCTG GACCAGCTGT GGAAGAACAA GGAATCTGGG 
CTAATGGCCT ACTCCCTGGC CCTTCTTAAC AATGTGAGCT ACAACGTGAT TGAGTTCGCC 
AAGTCGCAGA TAGAATGGAT GAGTGACAAG CTCACGAAGG CTTTCGAAGG TGCCCGCAAC 
AATCCCTTTC AGTTCAAGCA CATCCAGCTG TGCCATTCAC TGGCGGACGT CTATAAATTG 
CCCGCTGGTC CAAAAGTGGT GTTGGCCAGT ACCCCAGATC TGGAGAGCGG ATTCACGAGG 
GATCTCTTCG TGCAATGGGC CAGCAATGCC AACAATAGCA TAATCCTTAC TACTCGTACG 
TCTCCCGGCA CTTTAGCCAT GGAACTGGTG GAGAACTGCG CACCGGGCAA GCAAATCGAA 
TTGGACGTCA GGCGAAGAGT TGACTTAGAA GGAGCTGAGT TGGAGGAGTA CCTACGCACA 
CAGGGCGAGA AACTTAATCC CCTTATCGTA AAGCCCGATG TGGAGGAAGA GAGCAGTTCC 
GAGTCCGAGG ACGACATCGA GATGAGCGTC ATCACGGGCA AGCATGATAT TGTGGTCAGA 
CCGGAGGGTC GCCATCACTC CGGTTTTTTC AAGTCCAACA AACGACACCA CGTGATGTTC 
CCCTATCACG AGGAAAAGGT GAAGTGCGAT GAGTATGGGG AGATCATCAA TCTAGACGAC 
TACCGGATTG CGGATGCGAC AGGCTATGAG TTTGTACCTA TGGAGGAGCA AAACAAGGAG 
AATGTTAAAA AGGAAGAACC GGGCATAGGA GCAGAACAAC AGGCTAACGG AGGAATCGTC 
GACAATGATG TTCAGTTGCT GGAGAAGCCC ACCAAGCTGA TAAGCCAACG CAAAACAATC 
GAGGTGAATG CCCAAGTCCA GAGGATCGAT TTCGAAGGTC GCTCTGATGG CGAGTCCATG 
CTAAAGATCC TCTCCCAGTT GAGGCCACGA AGGGTTATCG TCATACACGG CACTGCCGAA 
GGCACACAAG TGGTGGCCAG GCACTGCGAA CAGAATGTCG GTGCGCGCGT TTTTACTCCG 
CAGAAGGGGG AAATCATTGA TGTCACCAGT GAGATTCACA TCTATCAGGT ACGCCTGACG 
GAAGGACTTG TCTCCCAGCT GCAGTTCCAA AAGGGCAAGG ACGCGGAGGT GGCGTGGGTG 
GATGGTCGCC TGGGCATGCG GGTGAAGGCC ATCGAAGCAC CCATGGACGT AACTGTGGAG 
CAGGACGCTT CCGTGCAAGA AGGCAAGACA TTGACTCTTG AAACACTGGC GGACGATGAG 
ATTCCTATCC ACAACTCAGT GCTCATTAAC GAGCTGAAAC TGTCCGACTT TAAGCAGACT 
CTCATGCGTA ATAACATCAA CTCGGAGTTC TCCGGCGGCG TGCTCTGGTG CAGCAACGGA 
ACTCTGGCCT TGAGGCGAGT GGATGCCGGC AAGGTAGCCA TGGAGGGATG CTTATCCGAG 
GAGTACTACA AGATCAGGGA GTTGCTGTAC GAGCAGTATG CGATCGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651658.1
CDS	91..2361
Translation	MTSIIKLHTISGAMDESPPCYILQIDDVRILLDCGWDEKFDANFIKELKRQVHTLDAVLLSHPDAYHLGALPYLVGKLGLNCPIYATIPVFKMGQMFMYDLYMSHFNMGDFDLFSLDDVDTAFEKITQLKYNQTVSLKDKGYGISITPLNAGHMIGGTIWKIVKVGEEDIVYATDFNHKKERHLSGCELDRLQRPSLLITDAYNAQYQQARRRARDEKLMTNILQTVRNNGNVLIAVDTAGRVLELAHMLDQLWKNKESGLMAYSLALLNNVSYNVIEFAKSQIEWMSDKLTKAFEGARNNPFQFKHIQLCHSLADVYKLPAGPKVVLASTPDLESGFTRDLFVQWASNANNSIILTTRTSPGTLAMELVENCAPGKQIELDVRRRVDLEGAELEEYLRTQGEKLNPLIVKPDVEEESSSESEDDIEMSVITGKHDIVVRPEGRHHSGFFKSNKRHHVMFPYHEEKVKCDEYGEIINLDDYRIADATGYEFVPMEEQNKENVKKEEPGIGAEQQANGGIVDNDVQLLEKPTKLISQRKTIEVNAQVQRIDFEGRSDGESMLKILSQLRPRRVIVIHGTAEGTQVVARHCEQNVGARVFTPQKGEIIDVTSEIHIYQVRLTEGLVSQLQFQKGKDAEVAWVDGRLGMRVKAIEAPMDVTVEQDASVQEGKTLTLETLADDEIPIHNSVLINELKLSDFKQTLMRNNINSEFSGGVLWCSNGTLALRRVDAGKVAMEGCLSEEYYKIRELLYEQYAIV

Target ORF information:

RefSeq Version	NM_143401.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cleavage and polyadenylation specificity factor 100 (Cpsf100), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143401.3

ORF Insert Sequence:

ATGACCTCCA TTATCAAGCT GCACACTATA TCTGGGGCGA TGGACGAGTC CCCGCCATGC 
TATATCCTAC AGATAGACGA TGTGCGGATA TTACTTGACT GCGGATGGGA CGAAAAGTTC 
GATGCCAACT TTATCAAGGA ACTGAAGAGG CAGGTGCACA CCCTGGACGC CGTGCTGCTA 
TCCCATCCGG ATGCATATCA TCTTGGAGCC CTGCCATACT TGGTGGGCAA GCTGGGGCTC 
AATTGCCCCA TCTACGCCAC CATTCCCGTG TTCAAAATGG GTCAAATGTT TATGTATGAC 
CTTTACATGT CGCACTTCAA TATGGGAGAC TTCGATCTAT TTTCGCTGGA CGACGTGGAC 
ACGGCTTTCG AAAAGATAAC CCAGTTGAAA TACAACCAAA CAGTTTCACT GAAGGACAAA 
GGATATGGAA TATCCATTAC GCCTTTGAAC GCTGGACACA TGATCGGCGG CACCATCTGG 
AAGATCGTCA AGGTGGGCGA GGAGGATATT GTGTACGCCA CCGACTTCAA CCATAAGAAG 
GAGCGCCACT TAAGTGGCTG CGAGCTGGAC AGGCTGCAAA GACCTTCGCT GCTCATTACC 
GACGCCTACA ACGCACAGTA CCAGCAGGCC AGAAGGAGGG CCCGCGATGA AAAGCTAATG 
ACCAACATCC TGCAAACGGT GCGTAACAAC GGCAATGTCC TGATAGCCGT GGATACGGCA 
GGACGAGTCC TGGAGTTGGC CCACATGCTG GACCAGCTGT GGAAGAACAA GGAATCTGGG 
CTAATGGCCT ACTCCCTGGC CCTTCTTAAC AATGTGAGCT ACAACGTGAT TGAGTTCGCC 
AAGTCGCAGA TAGAATGGAT GAGTGACAAG CTCACGAAGG CTTTCGAAGG TGCCCGCAAC 
AATCCCTTTC AGTTCAAGCA CATCCAGCTG TGCCATTCAC TGGCGGACGT CTATAAATTG 
CCCGCTGGTC CAAAAGTGGT GTTGGCCAGT ACCCCAGATC TGGAGAGCGG ATTCACGAGG 
GATCTCTTCG TGCAATGGGC CAGCAATGCC AACAATAGCA TAATCCTTAC TACTCGTACG 
TCTCCCGGCA CTTTAGCCAT GGAACTGGTG GAGAACTGCG CACCGGGCAA GCAAATCGAA 
TTGGACGTCA GGCGAAGAGT TGACTTAGAA GGAGCTGAGT TGGAGGAGTA CCTACGCACA 
CAGGGCGAGA AACTTAATCC CCTTATCGTA AAGCCCGATG TGGAGGAAGA GAGCAGTTCC 
GAGTCCGAGG ACGACATCGA GATGAGCGTC ATCACGGGCA AGCATGATAT TGTGGTCAGA 
CCGGAGGGTC GCCATCACTC CGGTTTTTTC AAGTCCAACA AACGACACCA CGTGATGTTC 
CCCTATCACG AGGAAAAGGT GAAGTGCGAT GAGTATGGGG AGATCATCAA TCTAGACGAC 
TACCGGATTG CGGATGCGAC AGGCTATGAG TTTGTACCTA TGGAGGAGCA AAACAAGGAG 
AATGTTAAAA AGGAAGAACC GGGCATAGGA GCAGAACAAC AGGCTAACGG AGGAATCGTC 
GACAATGATG TTCAGTTGCT GGAGAAGCCC ACCAAGCTGA TAAGCCAACG CAAAACAATC 
GAGGTGAATG CCCAAGTCCA GAGGATCGAT TTCGAAGGTC GCTCTGATGG CGAGTCCATG 
CTAAAGATCC TCTCCCAGTT GAGGCCACGA AGGGTTATCG TCATACACGG CACTGCCGAA 
GGCACACAAG TGGTGGCCAG GCACTGCGAA CAGAATGTCG GTGCGCGCGT TTTTACTCCG 
CAGAAGGGGG AAATCATTGA TGTCACCAGT GAGATTCACA TCTATCAGGT ACGCCTGACG 
GAAGGACTTG TCTCCCAGCT GCAGTTCCAA AAGGGCAAGG ACGCGGAGGT GGCGTGGGTG 
GATGGTCGCC TGGGCATGCG GGTGAAGGCC ATCGAAGCAC CCATGGACGT AACTGTGGAG 
CAGGACGCTT CCGTGCAAGA AGGCAAGACA TTGACTCTTG AAACACTGGC GGACGATGAG 
ATTCCTATCC ACAACTCAGT GCTCATTAAC GAGCTGAAAC TGTCCGACTT TAAGCAGACT 
CTCATGCGTA ATAACATCAA CTCGGAGTTC TCCGGCGGCG TGCTCTGGTG CAGCAACGGA 
ACTCTGGCCT TGAGGCGAGT GGATGCCGGC AAGGTAGCCA TGGAGGGATG CTTATCCGAG 
GAGTACTACA AGATCAGGGA GTTGCTGTAC GAGCAGTATG CGATCGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cpsf100 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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