CG4849 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG4849
Entrez Gene ID	43358
Full Name	CG4849 gene product from transcript CG4849-RA
Synonyms	Dmel\CG4849,EFTUD2,Snu114,eEF2,eftud2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98B6-98B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_143348.4	NP_651605.1	uncharacterized protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EFTUD2	XP_005624439.1
Saccharomyces cerevisiae (baker's yeast)	SNU114	NP_012748.1
Eremothecium gossypii	AGOS_AEL124W	NP_984737.1
Schizosaccharomyces pombe (fission yeast)	cwf10	NP_596689.2
Xenopus tropicalis (tropical clawed frog)	eftud2	NP_001025537.1
Homo sapiens (human)	EFTUD2	NP_001245282.1
Pan troglodytes (chimpanzee)	EFTUD2	XP_001145980.1
Oryza sativa (rice)	Os06g0608300	NP_001058038.1
Rattus norvegicus (Norway rat)	Eftud2	XP_213492.3
Arabidopsis thaliana (thale cress)	MEE5	NP_172112.1
Arabidopsis thaliana (thale cress)	AT5G25230	NP_197905.1
Mus musculus (house mouse)	Eftud2	NP_001103465.1
Danio rerio (zebrafish)	eftud2	NP_956802.2
Drosophila melanogaster (fruit fly)	CG4849	NP_651605.1
	EFTUD2	XP_001114964.1
Bos taurus (cattle)	EFTUD2	NP_001076865.1
Gallus gallus (chicken)	EFTUD2	NP_001026672.1
Caenorhabditis elegans (roundworm)	eftu-2	NP_498308.1
Kluyveromyces lactis	KLLA0A06688g	XP_451298.1
Magnaporthe oryzae (rice blast fungus)	MGG_13500	XP_003716278.1
Neurospora crassa	NCU02572	XP_965703.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG4849 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG4849 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06381	NM_143348.4 Latest version!	Drosophila melanogaster uncharacterized protein (CG4849), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG4849 ( NM_143348.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651605.1 Drosophila melanogaster uncharacterized protein (CG4849), mRNA.

Price & Availability↑

CloneID	OFa06381
Clone ID Related Accession (Same CDS sequence)	NM_143348.4
Accession Version	NM_143348.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143348.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTCCG ATTTGTACGA TGAGTTTGGC AACTATATTG GCCCCGATTT AGACAGCGAC 
GAGGAAGATG AGCAAAGTAT TTATGGGCAA CCCGATGTGC AGGATGACCC AGAGGACGCC 
ATGGACGAGG ACGAGGTGGA GCCACAGGAG GACGAGGACA AGGAGGTGAC CGCCGTGGTT 
TTGCACGAGG ACAAACGCTA CTATCCGTCA GCAGTTGAGG TCTACGGCCC GGATGTGGAG 
ACCATTGTCC AGGAGGAGGA CGCCCAGCCG CTGGACAAGC CGCTTATAGA GCCAGTGAAG 
AAGCTCAAGT TCCAGATCAA GGAGCAAGAC ATGCAGGAGA CCACCTACGA CATGGAGTTC 
ATGGCCGATC TCATGGACAC TCCGCCGCTT ATTCGCAACG TGGCTTTGGT TGGCCACCTG 
CACCACGGCA AGACCACCTT TGTCGACTGC CTCATCCGGC AGACGCATCC GCAATTTGAG 
ACCATGGAGG AGCGCCAGTT GCGCTACACG GACACCCTGT TCACGGAACA GGAACGCGGC 
TGCAGCATCA AGGCCACGCC GGTCACACTT GTCCTGCAGG ATGTCAAACA GAAGAGCTAC 
TTGCTGAACA TCTTCGACAC CCCGGGGCAT GTCAACTTCT CGGACGAGGC CACCGCCGCC 
ATGCGAATGA GCGATGGTGT CGTGCTGTTC ATCGATGCCG CCGAGGGCGT CATGCTCAAC 
ACGGAGCGAC TGCTCAAGCA CGCGGTGCAA GAGCGCCAGG CTATCACCGT TTGCATAAAC 
AAGATTGATC GCCTCATCCT GGAGCTGAAA TTGCCGCCCC AGGACGCCTA CTTTAAGTTG 
AAACACATTG TGGAGGAAGT CAATGGGCTG CTGAGCACCT ATGGCGCTCC AGATGACAAT 
CTCTTAGTTT CGCCTATTTT GGGCAACGTG TGCTTCGCCA GCTCGTTGTA CGGATTTTGT 
TTCACTCTGA AATCCTTTGC CAAGCTGTAT GCAGACACCT ATGAGGGAGT GGCTTACCTG 
GACTTTGCCA AGCGGCTCTG GGGTGACATG TACTTCAACA GTAAAACGCG CAAGTTCTCG 
AAGAAGCAGC CGCACAATTC GGCACAGCGC AGCTTTGTGG AGTTCATTTT GGAGCCCATG 
TACAAGCTGA TAGCCCAGGT GGTTGGCGAT GTGGACACCA CTTTGTCGGA CACTCTGGCC 
GAGCTGAATG TGCGCGTCTC CAAGGAGGAG ATGAAGTCCA ATATAAGACC ACTGCTGCGT 
CTCGTCTGTA ACCGCTTCAT GGGCGACTGC AGCGGTTTTG TGGATATGTG CGTGGAGCAC 
ATTAAATCCC CTTTGGAAAA TGCCAAGCGA AAGGTGGACC ATATATACAC TGGACCCAAA 
GAGGGTGACA TTTACCGGGA TATGATATCA TGCAACCAAT ACGGCACTCT GATGGTGCAC 
AGTTCCAAGA TGTATCCCAA CGACGACTGT ACCTTCTTCC AAGTGCTGGC GAGGATTGTC 
TCTGGTACTC TACACGCTGG CCAGGAGGTG CGTGTCCTGG GAGAGAACTA CACCTTGCAG 
GATGAGGAGG ACTCTCGCAT TCTGCAAGTC GGTCGTCTGT GGGTATTTGA GTCTCGTTAC 
AAGGTGGAGT TAAATCGTGT ACCCGCCGGA AATTGGGTGC TCATCGAAGG CATCGATCAG 
TGTATCGTTA AGACCTCCAC AATTGTGGAT ATTAATGTTC CAGAAGACCT CTACATATTC 
CGACCACTGA AGTTCAACAC CCAGAGCATT ATCAAGATTG CTGTGGAGCC AGTGAATCCA 
TCCGAGCTTC CCAAGATGTT GGATGGCCTC CGCAAGGTGA ATAAATCCTA TCCACTTCTC 
TCCACTCGAG TTGAAGAATC CGGCGAGCAT GTTATTCTGG GCACTGGCGA GCTGTACTTG 
GATTGTGTCA TGCACGATCT GCGGAAGATG TACTCCGAGA TCGACATCAA AGTGGCGGAT 
CCCGTCGTGG CTTTTTGCGA AACAGTTGTG GAAACGAGTT CCCTGAAATG TTTCGCCGAA 
ACGCCGAATA AGAAAAACAA AATAACCATG ATCTCGGAGC CACTGGAGAA GGGACTGGCC 
GAGGACATTG AGAACGGAAC CGTCTGCATA AACTGGAACA AAAAGCGCAT CGGCGAGTTC 
TTCCAAGTCA ACTACGATTG GGATCTGCTT GCGGCGCGTT CCATTTGGGC CTTCGGTCCC 
GACTCCACGG GTCCAAATAT CCTGGTCGAC GACACCCTGC CCTCGGAAGT GGACAAGAAC 
CTGCTGACCG CGGTTAAGGA TTCGATTGTG CAGGGCTTCC AATGGGGAAC GCGAGAAGGG 
CCGCTGTGCG AGGAGCCAAT CCGTAATGTC AAGTTTAAGA TTCTCGATGG CGTCATCGCC 
AACGAGGCGT TGCATCGAGG CGGTGGACAG ATCATTCCAA CAGCCCGTCG TGTGGCATAC 
TCGGCGTTTT TGATGGCCAC CCCACGTTTA ATGGAACCAT ATCTATTTGT GGAGGTTCAG 
GCGCCGGCAG ATTGTGTATC CGCTGTCTAT ACTGTGCTGG CCAGGCGCAG AGGCCACGTA 
ACGCAGGATG CTCCCGTATC GGGTTCTCCC ATATATACGA TCAAAGCCTT CATACCCGCC 
ATTGATTCAT TTGGATTTGA GACGGATCTC CGCACGCACA CCCAGGGCCA GGCCTTCTGC 
CTTTCGGTGT TCCATCACTG GCAGATAGTG CCTGGCGATC CCCTGGACAA GAGCATCATA 
ATCCGGCCAC TGGAGCCGCA GCAAGCATCC CATTTGGCCA GAGAGTTTAT GATCAAGACG 
CGTCGCAGGA AGGGTCTGTC CGAGGACGTG TCCATCAACA AATTCTTCGA CGATCCTATG 
TTGCTGGAGT TGGCACGCCA GGATGTGCTG ATCAACTACC CGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651605.1
CDS	148..3075
Translation	MDSDLYDEFGNYIGPDLDSDEEDEQSIYGQPDVQDDPEDAMDEDEVEPQEDEDKEVTAVVLHEDKRYYPSAVEVYGPDVETIVQEEDAQPLDKPLIEPVKKLKFQIKEQDMQETTYDMEFMADLMDTPPLIRNVALVGHLHHGKTTFVDCLIRQTHPQFETMEERQLRYTDTLFTEQERGCSIKATPVTLVLQDVKQKSYLLNIFDTPGHVNFSDEATAAMRMSDGVVLFIDAAEGVMLNTERLLKHAVQERQAITVCINKIDRLILELKLPPQDAYFKLKHIVEEVNGLLSTYGAPDDNLLVSPILGNVCFASSLYGFCFTLKSFAKLYADTYEGVAYLDFAKRLWGDMYFNSKTRKFSKKQPHNSAQRSFVEFILEPMYKLIAQVVGDVDTTLSDTLAELNVRVSKEEMKSNIRPLLRLVCNRFMGDCSGFVDMCVEHIKSPLENAKRKVDHIYTGPKEGDIYRDMISCNQYGTLMVHSSKMYPNDDCTFFQVLARIVSGTLHAGQEVRVLGENYTLQDEEDSRILQVGRLWVFESRYKVELNRVPAGNWVLIEGIDQCIVKTSTIVDINVPEDLYIFRPLKFNTQSIIKIAVEPVNPSELPKMLDGLRKVNKSYPLLSTRVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVVAFCETVVETSSLKCFAETPNKKNKITMISEPLEKGLAEDIENGTVCINWNKKRIGEFFQVNYDWDLLAARSIWAFGPDSTGPNILVDDTLPSEVDKNLLTAVKDSIVQGFQWGTREGPLCEEPIRNVKFKILDGVIANEALHRGGGQIIPTARRVAYSAFLMATPRLMEPYLFVEVQAPADCVSAVYTVLARRRGHVTQDAPVSGSPIYTIKAFIPAIDSFGFETDLRTHTQGQAFCLSVFHHWQIVPGDPLDKSIIIRPLEPQQASHLAREFMIKTRRRKGLSEDVSINKFFDDPMLLELARQDVLINYPL

Target ORF information:

RefSeq Version	NM_143348.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG4849), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143348.4

ORF Insert Sequence:

ATGGATTCCG ATTTGTACGA TGAGTTTGGC AACTATATTG GCCCCGATTT AGACAGCGAC 
GAGGAAGATG AGCAAAGTAT TTATGGGCAA CCCGATGTGC AGGATGACCC AGAGGACGCC 
ATGGACGAGG ACGAGGTGGA GCCACAGGAG GACGAGGACA AGGAGGTGAC CGCCGTGGTT 
TTGCACGAGG ACAAACGCTA CTATCCGTCA GCAGTTGAGG TCTACGGCCC GGATGTGGAG 
ACCATTGTCC AGGAGGAGGA CGCCCAGCCG CTGGACAAGC CGCTTATAGA GCCAGTGAAG 
AAGCTCAAGT TCCAGATCAA GGAGCAAGAC ATGCAGGAGA CCACCTACGA CATGGAGTTC 
ATGGCCGATC TCATGGACAC TCCGCCGCTT ATTCGCAACG TGGCTTTGGT TGGCCACCTG 
CACCACGGCA AGACCACCTT TGTCGACTGC CTCATCCGGC AGACGCATCC GCAATTTGAG 
ACCATGGAGG AGCGCCAGTT GCGCTACACG GACACCCTGT TCACGGAACA GGAACGCGGC 
TGCAGCATCA AGGCCACGCC GGTCACACTT GTCCTGCAGG ATGTCAAACA GAAGAGCTAC 
TTGCTGAACA TCTTCGACAC CCCGGGGCAT GTCAACTTCT CGGACGAGGC CACCGCCGCC 
ATGCGAATGA GCGATGGTGT CGTGCTGTTC ATCGATGCCG CCGAGGGCGT CATGCTCAAC 
ACGGAGCGAC TGCTCAAGCA CGCGGTGCAA GAGCGCCAGG CTATCACCGT TTGCATAAAC 
AAGATTGATC GCCTCATCCT GGAGCTGAAA TTGCCGCCCC AGGACGCCTA CTTTAAGTTG 
AAACACATTG TGGAGGAAGT CAATGGGCTG CTGAGCACCT ATGGCGCTCC AGATGACAAT 
CTCTTAGTTT CGCCTATTTT GGGCAACGTG TGCTTCGCCA GCTCGTTGTA CGGATTTTGT 
TTCACTCTGA AATCCTTTGC CAAGCTGTAT GCAGACACCT ATGAGGGAGT GGCTTACCTG 
GACTTTGCCA AGCGGCTCTG GGGTGACATG TACTTCAACA GTAAAACGCG CAAGTTCTCG 
AAGAAGCAGC CGCACAATTC GGCACAGCGC AGCTTTGTGG AGTTCATTTT GGAGCCCATG 
TACAAGCTGA TAGCCCAGGT GGTTGGCGAT GTGGACACCA CTTTGTCGGA CACTCTGGCC 
GAGCTGAATG TGCGCGTCTC CAAGGAGGAG ATGAAGTCCA ATATAAGACC ACTGCTGCGT 
CTCGTCTGTA ACCGCTTCAT GGGCGACTGC AGCGGTTTTG TGGATATGTG CGTGGAGCAC 
ATTAAATCCC CTTTGGAAAA TGCCAAGCGA AAGGTGGACC ATATATACAC TGGACCCAAA 
GAGGGTGACA TTTACCGGGA TATGATATCA TGCAACCAAT ACGGCACTCT GATGGTGCAC 
AGTTCCAAGA TGTATCCCAA CGACGACTGT ACCTTCTTCC AAGTGCTGGC GAGGATTGTC 
TCTGGTACTC TACACGCTGG CCAGGAGGTG CGTGTCCTGG GAGAGAACTA CACCTTGCAG 
GATGAGGAGG ACTCTCGCAT TCTGCAAGTC GGTCGTCTGT GGGTATTTGA GTCTCGTTAC 
AAGGTGGAGT TAAATCGTGT ACCCGCCGGA AATTGGGTGC TCATCGAAGG CATCGATCAG 
TGTATCGTTA AGACCTCCAC AATTGTGGAT ATTAATGTTC CAGAAGACCT CTACATATTC 
CGACCACTGA AGTTCAACAC CCAGAGCATT ATCAAGATTG CTGTGGAGCC AGTGAATCCA 
TCCGAGCTTC CCAAGATGTT GGATGGCCTC CGCAAGGTGA ATAAATCCTA TCCACTTCTC 
TCCACTCGAG TTGAAGAATC CGGCGAGCAT GTTATTCTGG GCACTGGCGA GCTGTACTTG 
GATTGTGTCA TGCACGATCT GCGGAAGATG TACTCCGAGA TCGACATCAA AGTGGCGGAT 
CCCGTCGTGG CTTTTTGCGA AACAGTTGTG GAAACGAGTT CCCTGAAATG TTTCGCCGAA 
ACGCCGAATA AGAAAAACAA AATAACCATG ATCTCGGAGC CACTGGAGAA GGGACTGGCC 
GAGGACATTG AGAACGGAAC CGTCTGCATA AACTGGAACA AAAAGCGCAT CGGCGAGTTC 
TTCCAAGTCA ACTACGATTG GGATCTGCTT GCGGCGCGTT CCATTTGGGC CTTCGGTCCC 
GACTCCACGG GTCCAAATAT CCTGGTCGAC GACACCCTGC CCTCGGAAGT GGACAAGAAC 
CTGCTGACCG CGGTTAAGGA TTCGATTGTG CAGGGCTTCC AATGGGGAAC GCGAGAAGGG 
CCGCTGTGCG AGGAGCCAAT CCGTAATGTC AAGTTTAAGA TTCTCGATGG CGTCATCGCC 
AACGAGGCGT TGCATCGAGG CGGTGGACAG ATCATTCCAA CAGCCCGTCG TGTGGCATAC 
TCGGCGTTTT TGATGGCCAC CCCACGTTTA ATGGAACCAT ATCTATTTGT GGAGGTTCAG 
GCGCCGGCAG ATTGTGTATC CGCTGTCTAT ACTGTGCTGG CCAGGCGCAG AGGCCACGTA 
ACGCAGGATG CTCCCGTATC GGGTTCTCCC ATATATACGA TCAAAGCCTT CATACCCGCC 
ATTGATTCAT TTGGATTTGA GACGGATCTC CGCACGCACA CCCAGGGCCA GGCCTTCTGC 
CTTTCGGTGT TCCATCACTG GCAGATAGTG CCTGGCGATC CCCTGGACAA GAGCATCATA 
ATCCGGCCAC TGGAGCCGCA GCAAGCATCC CATTTGGCCA GAGAGTTTAT GATCAAGACG 
CGTCGCAGGA AGGGTCTGTC CGAGGACGTG TCCATCAACA AATTCTTCGA CGATCCTATG 
TTGCTGGAGT TGGCACGCCA GGATGTGCTG ATCAACTACC CGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG4849 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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