wdb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wdb
Entrez Gene ID	43312
Full Name	widerborst
Synonyms	0318/07,B56-2,BEST:LD02456,BcDNA:LD34343,CG5643,Dmel\CG5643,EP3559,LD02456,PP2-AB,PP2A,PP2A-B',PP2A[B'-2],PP2a,WDB,Wdb,dB56-2,dPP2A,dPP2A-B56-2,l(3)S031807
General protein information	Preferred Names widerborst Names CG5643 gene product from transcript CG5643-RD CG5643-PA CG5643-PB CG5643-PC CG5643-PD CG5643-PE CG5643-PF PP2A B subunit PP2A regulatory subunit protein phosphatase 2a protein phosphatase PP2A wdb-PA wdb-PB wdb-PC wdb-PD wdb-PE wdb-PF wiederborst
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98A6-98A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_170338.3	NP_733217.1	widerborst, isoform D
NM_170336.3	NP_733215.1	widerborst, isoform A
NM_170339.3	NP_733218.1	widerborst, isoform E
NM_170340.2	NP_733219.1	widerborst, isoform F
NM_170337.3	NP_733216.1	widerborst, isoform B
NM_143312.4	NP_651569.1	widerborst, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PPP2R5E	XP_003314413.1
Rattus norvegicus (Norway rat)	Ppp2r5e	NP_001100210.1
Danio rerio (zebrafish)	ppp2r5ea	NP_919396.1
Bos taurus (cattle)	PPP2R5E	NP_001076937.1
Caenorhabditis elegans (roundworm)	pptr-1	NP_507133.4
Arabidopsis thaliana (thale cress)	ATB' ALPHA	NP_195967.1
Drosophila melanogaster (fruit fly)	wdb	NP_733216.1
Arabidopsis thaliana (thale cress)	AT3G54930	NP_191053.1
Arabidopsis thaliana (thale cress)	ATB' BETA	NP_187599.1
Homo sapiens (human)	PPP2R5E	NP_001269108.1
	PPP2R5E	XP_002805110.1
Canis lupus familiaris (dog)	PPP2R5E	XP_005623536.1
Mus musculus (house mouse)	Ppp2r5e	NP_036154.1
Gallus gallus (chicken)	PPP2R5E	XP_421412.2
Danio rerio (zebrafish)	ppp2r5eb	NP_919393.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004096	XP_003436712.1
Xenopus tropicalis (tropical clawed frog)	ppp2r5e	NP_989253.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies identify PP2A-Wdb as a participant in Notch signaling, and suggest that M8 activity is controlled by phosphorylation and dephosphorylation.
Title: The Ser/Thr phosphatase PP2A regulatory subunit widerborst inhibits notch signaling.

wdb- and lkb1-dependent lifespan extension is mediated by downregulation of S6K, a downstream component of the insulin/IGF and TOR signaling pathways.
Title: A gain-of-function screen identifies wdb and lkb1 as lifespan-extending genes in Drosophila.

The protein phosphatase PP2A-B' subunit Widerborst is a negative regulator of cytoplasmic activated Akt and lipid metabolism in Drosophila
Title: The protein phosphatase PP2A-B' subunit Widerborst is a negative regulator of cytoplasmic activated Akt and lipid metabolism in Drosophila.

posttranslational mechanisms that drive cycling of PER require the rhythmic expression of PP2A (wdb and tws)
Title: Posttranslational regulation of Drosophila PERIOD protein by protein phosphatase 2A.

Planar cell polarization of wing epithelial cells requires Widerborst, a B' regulatory subunit of protein phosphatase 2A.
Title: Planar cell polarization requires Widerborst, a B' regulatory subunit of protein phosphatase 2A.

The following wdb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wdb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06337	NM_170338.3 Latest version!	Drosophila melanogaster widerborst (wdb), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06337	NM_170337.3 Latest version!	Drosophila melanogaster widerborst (wdb), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06337	NM_170340.2 Latest version!	Drosophila melanogaster widerborst (wdb), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06337	NM_143312.4 Latest version!	Drosophila melanogaster widerborst (wdb), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06337	NM_170336.3 Latest version!	Drosophila melanogaster widerborst (wdb), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06337	NM_170339.3 Latest version!	Drosophila melanogaster widerborst (wdb), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wdb ( NM_170338.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733217.1 Drosophila melanogaster widerborst (wdb), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa06337
Clone ID Related Accession (Same CDS sequence)	NM_170338.3 , NM_170336.3 , NM_170339.3 , NM_170340.2 , NM_170337.3 , NM_143312.4
Accession Version	NM_170338.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	widerborst, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170338.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733217.1
CDS	1374..2948
Translation	MSSGTFVDRIDPFAKRSLKKKGKKSQGSSRYRNSQDVELQQLPPLKADCSSLEQEELFIRKLRQCCVSFDFMDPVTDLKGKEIKRAALNDLSTYITHGRGVLTEPVYPEIIRMISCNLFRTLPPSENPDFDPEEDDPTLEASWPHLQLVYEVFLRFLESQDFQATIGKRVIDQKFVLQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVGELLEILGSIINGFALPLKAEHKQFLVKVLLPLHKVKCLSLYHAQLAYCIVQFLEKDPFLTEPVVRGLLKFWPKTCSQKEVMFLGEIEEILDVIDPPQFVKIQEPLFRQIAKCVSSPHFQVAERALYLWNNEYAMSLIEENNAVIMPIMFPALYRISKEHWNQTIVALVYNVLKTFMEMNSKLFDELTSSYKAERQKEKKRERDREELWKKLHELESNRSSGRTAGGSATTSNSAASAASTSLQPPSSAGLNSHQQQSNSGSSGSLSSGGAGGDNNPATTNAKIKQDKADN

Target ORF information:

RefSeq Version	NM_170338.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster widerborst (wdb), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170338.3

ORF Insert Sequence:

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wdb ( NM_170336.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733215.1 Drosophila melanogaster widerborst (wdb), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06337
Clone ID Related Accession (Same CDS sequence)	NM_170338.3 , NM_170336.3 , NM_170339.3 , NM_170340.2 , NM_170337.3 , NM_143312.4
Accession Version	NM_170336.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	widerborst, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170336.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733215.1
CDS	1526..3100
Translation	MSSGTFVDRIDPFAKRSLKKKGKKSQGSSRYRNSQDVELQQLPPLKADCSSLEQEELFIRKLRQCCVSFDFMDPVTDLKGKEIKRAALNDLSTYITHGRGVLTEPVYPEIIRMISCNLFRTLPPSENPDFDPEEDDPTLEASWPHLQLVYEVFLRFLESQDFQATIGKRVIDQKFVLQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVGELLEILGSIINGFALPLKAEHKQFLVKVLLPLHKVKCLSLYHAQLAYCIVQFLEKDPFLTEPVVRGLLKFWPKTCSQKEVMFLGEIEEILDVIDPPQFVKIQEPLFRQIAKCVSSPHFQVAERALYLWNNEYAMSLIEENNAVIMPIMFPALYRISKEHWNQTIVALVYNVLKTFMEMNSKLFDELTSSYKAERQKEKKRERDREELWKKLHELESNRSSGRTAGGSATTSNSAASAASTSLQPPSSAGLNSHQQQSNSGSSGSLSSGGAGGDNNPATTNAKIKQDKADN

Target ORF information:

RefSeq Version	NM_170336.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster widerborst (wdb), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170336.3

ORF Insert Sequence:

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wdb ( NM_170339.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733218.1 Drosophila melanogaster widerborst (wdb), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa06337
Clone ID Related Accession (Same CDS sequence)	NM_170338.3 , NM_170336.3 , NM_170339.3 , NM_170340.2 , NM_170337.3 , NM_143312.4
Accession Version	NM_170339.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	widerborst, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170339.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733218.1
CDS	1534..3108
Translation	MSSGTFVDRIDPFAKRSLKKKGKKSQGSSRYRNSQDVELQQLPPLKADCSSLEQEELFIRKLRQCCVSFDFMDPVTDLKGKEIKRAALNDLSTYITHGRGVLTEPVYPEIIRMISCNLFRTLPPSENPDFDPEEDDPTLEASWPHLQLVYEVFLRFLESQDFQATIGKRVIDQKFVLQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVGELLEILGSIINGFALPLKAEHKQFLVKVLLPLHKVKCLSLYHAQLAYCIVQFLEKDPFLTEPVVRGLLKFWPKTCSQKEVMFLGEIEEILDVIDPPQFVKIQEPLFRQIAKCVSSPHFQVAERALYLWNNEYAMSLIEENNAVIMPIMFPALYRISKEHWNQTIVALVYNVLKTFMEMNSKLFDELTSSYKAERQKEKKRERDREELWKKLHELESNRSSGRTAGGSATTSNSAASAASTSLQPPSSAGLNSHQQQSNSGSSGSLSSGGAGGDNNPATTNAKIKQDKADN

Target ORF information:

RefSeq Version	NM_170339.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster widerborst (wdb), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170339.3

ORF Insert Sequence:

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wdb ( NM_170340.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733219.1 Drosophila melanogaster widerborst (wdb), transcript variant F, mRNA.

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CloneID	OFa06337
Clone ID Related Accession (Same CDS sequence)	NM_170338.3 , NM_170336.3 , NM_170339.3 , NM_170340.2 , NM_170337.3 , NM_143312.4
Accession Version	NM_170340.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	widerborst, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170340.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733219.1
CDS	1618..3192
Translation	MSSGTFVDRIDPFAKRSLKKKGKKSQGSSRYRNSQDVELQQLPPLKADCSSLEQEELFIRKLRQCCVSFDFMDPVTDLKGKEIKRAALNDLSTYITHGRGVLTEPVYPEIIRMISCNLFRTLPPSENPDFDPEEDDPTLEASWPHLQLVYEVFLRFLESQDFQATIGKRVIDQKFVLQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVGELLEILGSIINGFALPLKAEHKQFLVKVLLPLHKVKCLSLYHAQLAYCIVQFLEKDPFLTEPVVRGLLKFWPKTCSQKEVMFLGEIEEILDVIDPPQFVKIQEPLFRQIAKCVSSPHFQVAERALYLWNNEYAMSLIEENNAVIMPIMFPALYRISKEHWNQTIVALVYNVLKTFMEMNSKLFDELTSSYKAERQKEKKRERDREELWKKLHELESNRSSGRTAGGSATTSNSAASAASTSLQPPSSAGLNSHQQQSNSGSSGSLSSGGAGGDNNPATTNAKIKQDKADN

Target ORF information:

RefSeq Version	NM_170340.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster widerborst (wdb), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170340.2

ORF Insert Sequence:

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wdb ( NM_170337.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733216.1 Drosophila melanogaster widerborst (wdb), transcript variant B, mRNA.

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CloneID	OFa06337
Clone ID Related Accession (Same CDS sequence)	NM_170338.3 , NM_170336.3 , NM_170339.3 , NM_170340.2 , NM_170337.3 , NM_143312.4
Accession Version	NM_170337.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	widerborst, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170337.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733216.1
CDS	1205..2779
Translation	MSSGTFVDRIDPFAKRSLKKKGKKSQGSSRYRNSQDVELQQLPPLKADCSSLEQEELFIRKLRQCCVSFDFMDPVTDLKGKEIKRAALNDLSTYITHGRGVLTEPVYPEIIRMISCNLFRTLPPSENPDFDPEEDDPTLEASWPHLQLVYEVFLRFLESQDFQATIGKRVIDQKFVLQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVGELLEILGSIINGFALPLKAEHKQFLVKVLLPLHKVKCLSLYHAQLAYCIVQFLEKDPFLTEPVVRGLLKFWPKTCSQKEVMFLGEIEEILDVIDPPQFVKIQEPLFRQIAKCVSSPHFQVAERALYLWNNEYAMSLIEENNAVIMPIMFPALYRISKEHWNQTIVALVYNVLKTFMEMNSKLFDELTSSYKAERQKEKKRERDREELWKKLHELESNRSSGRTAGGSATTSNSAASAASTSLQPPSSAGLNSHQQQSNSGSSGSLSSGGAGGDNNPATTNAKIKQDKADN

Target ORF information:

RefSeq Version	NM_170337.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster widerborst (wdb), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170337.3

ORF Insert Sequence:

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wdb ( NM_143312.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651569.1 Drosophila melanogaster widerborst (wdb), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06337
Clone ID Related Accession (Same CDS sequence)	NM_170338.3 , NM_170336.3 , NM_170339.3 , NM_170340.2 , NM_170337.3 , NM_143312.4
Accession Version	NM_143312.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	widerborst, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143312.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651569.1
CDS	1365..2939
Translation	MSSGTFVDRIDPFAKRSLKKKGKKSQGSSRYRNSQDVELQQLPPLKADCSSLEQEELFIRKLRQCCVSFDFMDPVTDLKGKEIKRAALNDLSTYITHGRGVLTEPVYPEIIRMISCNLFRTLPPSENPDFDPEEDDPTLEASWPHLQLVYEVFLRFLESQDFQATIGKRVIDQKFVLQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVGELLEILGSIINGFALPLKAEHKQFLVKVLLPLHKVKCLSLYHAQLAYCIVQFLEKDPFLTEPVVRGLLKFWPKTCSQKEVMFLGEIEEILDVIDPPQFVKIQEPLFRQIAKCVSSPHFQVAERALYLWNNEYAMSLIEENNAVIMPIMFPALYRISKEHWNQTIVALVYNVLKTFMEMNSKLFDELTSSYKAERQKEKKRERDREELWKKLHELESNRSSGRTAGGSATTSNSAASAASTSLQPPSSAGLNSHQQQSNSGSSGSLSSGGAGGDNNPATTNAKIKQDKADN

Target ORF information:

RefSeq Version	NM_143312.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster widerborst (wdb), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143312.4

ORF Insert Sequence:

ATGTCATCGG GCACGTTTGT GGATCGAATC GACCCGTTCG CCAAACGTTC GCTCAAGAAG 
AAGGGTAAAA AGAGTCAAGG TTCCTCGCGC TACAGAAATT CTCAGGATGT TGAGCTGCAG 
CAATTGCCGC CGCTGAAAGC TGATTGCTCC AGCCTAGAAC AGGAGGAGCT GTTCATAAGG 
AAGCTGCGCC AGTGTTGCGT GTCCTTTGAC TTTATGGATC CCGTGACGGA CTTGAAGGGC 
AAGGAGATCA AACGGGCCGC GCTCAATGAC CTATCCACCT ATATAACACA TGGGCGCGGC 
GTGCTTACGG AACCGGTTTA TCCGGAAATA ATTCGTATGA TTTCTTGCAA CCTATTTCGC 
ACGTTGCCGC CCAGTGAGAA CCCTGATTTC GATCCCGAGG AGGACGATCC GACACTGGAG 
GCGTCCTGGC CACACCTCCA ACTGGTGTAC GAGGTCTTCT TGCGGTTTCT GGAGTCGCAG 
GATTTCCAAG CAACTATCGG CAAGCGTGTG ATCGATCAAA AGTTTGTCTT GCAGCTTTTG 
GAACTTTTCG ACTCGGAGGA TCCCAGGGAG CGGGACTTTC TGAAGACGGT ATTGCATCGT 
ATATATGGAA AATTCTTAGG ACTGCGGGCT TTTATACGAA AACAGATTAA CAACATATTC 
TTGCGATTCA TTTACGAAAC GGAGCACTTT AATGGAGTCG GTGAGCTCTT GGAAATCCTT 
GGCAGTATCA TCAACGGATT CGCCTTGCCC CTGAAGGCCG AACACAAACA GTTTCTGGTT 
AAGGTCCTAT TGCCACTGCA CAAAGTCAAA TGCCTGTCGC TCTACCACGC TCAACTGGCG 
TATTGCATTG TACAATTCCT AGAGAAGGAT CCATTCCTTA CCGAACCGGT GGTGCGTGGC 
CTGCTGAAGT TCTGGCCCAA GACGTGTTCC CAGAAGGAGG TCATGTTCCT GGGCGAGATT 
GAGGAGATCC TCGATGTGAT CGATCCGCCG CAGTTTGTCA AGATTCAGGA GCCGCTATTC 
CGTCAGATTG CAAAATGCGT GTCGAGTCCA CATTTTCAGG TTGCTGAGCG AGCTCTTTAT 
CTGTGGAACA ATGAGTACGC CATGTCGCTA ATCGAGGAGA ACAATGCGGT CATCATGCCG 
ATCATGTTCC CGGCCCTATA CCGCATCAGC AAGGAACACT GGAATCAAAC TATCGTGGCG 
CTCGTATACA ACGTTTTGAA GACGTTCATG GAGATGAATT CGAAACTGTT CGACGAGCTT 
ACCTCCAGCT ACAAGGCGGA GCGGCAAAAG GAAAAAAAAC GTGAGCGTGA CCGCGAGGAA 
CTGTGGAAGA AACTGCACGA ACTCGAATCC AATCGTTCTA GTGGGCGCAC CGCCGGCGGC 
AGCGCCACGA CATCGAACAG CGCAGCTTCC GCGGCGTCAA CGTCCCTGCA GCCACCCAGC 
TCCGCTGGCC TGAACTCTCA TCAGCAGCAG TCGAATAGCG GCAGCAGCGG TAGCCTGTCC 
AGCGGCGGTG CCGGCGGCGA CAATAATCCT GCGACCACAA ATGCGAAAAT CAAACAGGAT 
AAGGCGGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wdb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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