l(3)mbt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	l(3)mbt
Entrez Gene ID	43288
Full Name	lethal (3) malignant brain tumor
Synonyms	CG5954,Dmel\CG5954,L(3)MBT,L(3)MBT/LIN-61,L(3)mbt,L3mbt,MBT,dL(3)mbt,dl(3)MBT,l(3)malignant braintumor,mbt
General protein information	Preferred Names lethal (3) malignant brain tumor Names CG5954 gene product from transcript CG5954-RB CG5954-PB l(3)mbt-PB tumor-supressor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 97F1-97F1\|3-93.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_170330.2	NP_733209.1	lethal (3) malignant brain tumor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	L3MBTL3	XP_419742.4
Homo sapiens (human)	L3MBTL3	NP_115814.1
Pan troglodytes (chimpanzee)	L3MBTL3	XP_518737.2
Mus musculus (house mouse)	L3mbtl3	NP_766375.1
Canis lupus familiaris (dog)	L3MBTL3	XP_003638818.1
Bos taurus (cattle)	L3MBTL3	NP_001070339.1
Danio rerio (zebrafish)	l3mbtl1b	XP_688020.5
	L3MBTL3	XP_001104830.2
Rattus norvegicus (Norway rat)	L3mbtl3	XP_006227760.1
Drosophila melanogaster (fruit fly)	l(3)mbt	NP_733209.1
Xenopus tropicalis (tropical clawed frog)	l3mbtl3	XP_002939531.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CRISPR-mediated loss of function of lethal (3) malignant brain tumor [l(3)mbt] leads to ectopic activation of the germ-specific ping-pong cycle in ovarian somatic cells
Title: Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells.

Data show that L(3)mbt chromosomal targeting requires proteins of a site-specific DNA binding complex Myb-MuvB (MMB core component Mip130)/DREAM.
Title: Chromatin reader L(3)mbt requires the Myb-MuvB/DREAM transcriptional regulatory complex for chromosomal recruitment.

Data indicate that germline genes, some of which drive oncogenesis in Drosophila melanogaster, are similarly ectopically activated in a wide range of human cancers.
Title: Meta-analysis of expression of l(3)mbt tumor-associated germline genes supports the model that a soma-to-germline transition is a hallmark of human cancers.

Data show that brain tumours in l(3)mbt mutants originate from overproliferation of neuroepithelial cells in the optic lobes caused by derepression of target genes in the Salvador-Warts-Hippo (SWH) pathway.
Title: The tumour suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements.

The following l(3)mbt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(3)mbt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06316	NM_170330.2 Latest version!	Drosophila melanogaster lethal (3) malignant brain tumor (l(3)mbt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

l(3)mbt ( NM_170330.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733209.1 Drosophila melanogaster lethal (3) malignant brain tumor (l(3)mbt), mRNA.

Price & Availability↑

CloneID	OFa06316
Clone ID Related Accession (Same CDS sequence)	NM_170330.2
Accession Version	NM_170330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (3) malignant brain tumor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170330.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCCAT TGTCGATGGC CAGTGCGGAG GCCAAGCAGC TCCAGCGCAA TATATCATTG 
TCCATGTCGC CAGCGAATGG AGGAGTCATC TCACCATCTA CCGCGAATTG CAATGGTGTT 
GCCTCTGCCT CCGGCGGAAA TCCAAATCCC AACAGCCTCC TCACACTAGC TCCGCGCAAG 
GAGGAGCTAC GCTTCCTGCC CCTGGCGCCC ATGGGCGGCA ACAAGACCTG CAACATCACC 
ATCAGCAATG TCCAGCCAAT GAAATCCAAA CTAAAGTCGG TCAACATAGT GCCAAATTCC 
ATAAAGTCCG CTGGAGCTGG CGCCACTGTC GGATCAGCAG ATGCTCCCCT GTTCCAGCTG 
GTGCCATCGC CCAGTAATCC CAGCCAACCG TTGGTGGCCA TTCTGGCCCC CAATCGCAAG 
ACCACGCTGC TTGGCGGAGG TGCATCCAAT GGCCAACCGG TGGTCATACG CTCCGGAACA 
CCTTTGCACA AGAACAGCCT GACCGTTGAG ACCAGCAACA AGCCCATCCA GCCAGGAGGA 
TCGGCTGCAG CAGCCGCCGT TGGAGGCGGA ACTGGAGGAG CACCTTTAAC GCTCGTGGGC 
AAAACGGTCG TTCCACCGAA AATACAAGCG GCGACACCTG TCCAGATTCC CAGCACGGGT 
CCGGGTGCGC AGGTATCCCT GCTGGCCAAT CCCCTCAAGC CACGTGCTCC ACTCATACTC 
AGCAAGGTGG CGGTGGATAA GCTGCGTCTA AAGTTCAGCC AGGTCAAGAG CAACCCAAAT 
GCACTCGTTC TCGGCAAGGC CAATCATGTG AAGAAACTTC TGACATCGCC GAACCCCAGT 
GGAGAGGATA AGACCAGGAG CACCCAGAAA AACAACAAAC AGAATACTAG TGCCAGCCAA 
CTGAAGCCAG TTGTGGCACC CAAAACGGCT ATGCCCATTG CCACGGAGGT GCCGACCGCC 
GATAACAACA AAATGAGCCT AATTAAGTCC AAGCCCGTGG CCATCAAGGC AGTGCCGCTG 
CCCAGCCAGG AAGCGCCCAT TGTGCCGGCA GTGGCGCCAG TTGTTACATC TCCAGAGAAG 
GTCGACGTTA AGCCCACTGC AAAGCCCTCA ATCAAGACAG CACCCAAACC AACTCCTAAA 
CCTCTGGAGA TGTCCGTAAA TGGACCAGCC AAGGAGAAAA AGGCGCCTGC CAAGGAGCAG 
AAACAGAAGA CAATGGACAA ACCACCGGTG GTCAAGGAAA TCGCTCAACA TACGCCATGT 
GGGCCCAAGG AATTGCCCAA ACTAAAGCGG TCGAAATCCT TCGTGTCCAA CCATCCCGCT 
TCGCCTGTGG CCAACAACGA GCGACGTCAC TCGGTGGCCA TTATGGCCAA GGAGGTGGAT 
GTGATCGAGC AGCCCAGTGC GGCAATCGAG ACCATCACCA TTGAAGACGA TGATGAGAGC 
GAGGAAGAGC AGCAGAAGCC GGAAATTAAG AAGACGCCGA AGCAGAATCA GAAGCCGGAA 
AAGCAGGAGG CGCCGAAGAA GAAGCAGATC CCCATGCCCA TTCTGCCCGC AGGAATAACC 
ATTTCCACCA CCAGTACGGC CAAGAAAAAG GCCGTGAGAA AGAACTCCTC CGTGACGACA 
ATCTCTTCTA GTTCCAGCGA CAGTTCGTCC TACAGCGATG TGGAGGTGGT ACCGACCAAG 
AAAACCGAGA AGGAACTCAG TATGCTGCCT GGCATAAGCA TCATCAAGGC GGAATCTCTG 
CCACTGAGCA GGGAGGACTT TGAACGCTCG TTGCGTTGCG ATGAGCAGGT GCCCCAAACT 
CCGCCCAAGT CATCCTCCTC ATCCTCGAGC GGCTCTCACG CATCGGAAAT GTCGCCGCCG 
CCCAAACTCG CCGATCCGAA GCCATCAAGT CCACCTGCAA AGAATGCCAA GAAAGCCAAC 
GACCATGATG CGAACCCATC TCCTAGCAGC AAGACCAATG ACTTTAAAAA GAGCATGATG 
CAGAACCTGA ACATGTTGAA ATGGCGCGGC CAGCAGCCGG CCAACCTGCA GAACTCCACC 
GTCCGGTTCG AGCTGAATGA GTTCAATTTT CTGCAGATAA ACGAACGCTG CCAGCCACGC 
CAGGGTCCAG CTGCATATTT CGAGCGGCCT TTGTATGATC GTCCGGGCCG CAGGCCCTCG 
GCCTGCGTCC ATCCGCTGCT GTACTTGTGC CAGCGTTGCA ATTTCCACGG ACCCGCCGCC 
GACTTTCTGG CACCACATTT CTGCTCCGTT GGCTGTGTGC GTCGATCCCA GAAGCGTCGT 
CTGCCGCAGA GTACGCAGAA GGAGAGCAAG ATAAGCCGTA CCCAACTAGA GCAAACAGCT 
GGAACAGCGC CAGATTTGCC ACAAAAGCGG CAAACCAACT ACAAACCACA CAAATCTCTG 
TCTGCCATAC AGGAACAGCT GCAGCAACGT CAAAAGGATA AGCCCAGAAA GCCATTCCGC 
TGGAGCGAGT ACCTCAAGTC AAAGGGAAAG GATGTGGCAG CGCCCATCCA CCTGTTCCTC 
AATCCGTTCC CCATCAGTCC CAACTGCTTC GAGATCGGCA TGAAGCTAGA GGCCATCGAT 
CCGGAGAACT GCTCGCTCTT CTGTGTCTGC AGCATTGTCG AGGTGCGCGG CTATCGGCTG 
AAGCTTAGCT TCGATGGCTA CTCCTCCATG TACGACTTCT GGGTGAACGC CGACTCGCAG 
GACATCTTTC CGCCAGGCTG GTGCGATGAG ACAGCACGCG TTCTGCAGGC GCCTAAGGAC 
TACAACTCGG AGCGCTTCAG TTGGAGCCGC TATTTGGTGA AAACGGGCGG CAAGGCAGCT 
CCGCGGGCGC TCTTTGGTCA TCTGAATATG CAGCAGCAGA TGGATGTGCG CAACGGCTTC 
GCTGTGGGCA TGCATCTCGA GGCGGAGGAC CTGAACGATA CGGGAAAGAT TTGTGTGGCC 
ACAGTGACGG ATATTCTGGA CGAGCGCATT CGAGTCCACT TCGACGGCTG GGATGACTGC 
TACGACCTGT GGGTACACAT CACCTCACCG TACATCCATC CCTGCGGTTG GCACGAGGGA 
CGCCAGCAGT TGATCGTTCC GCCGGACTAC CAGAAGTCTG CCTTCATTTG GGATGACTAC 
ATATCAGAGG TTGGTGGAAT GGCCGCCTCC AAGGAGCTGT TCACACCACG CCAGCCAATG 
GAGTACCAGG AACGCATGAA ACTGGAGGTG GTGGACCAGC GTAATCCCTG CCTAATTCGT 
CCGGCCACCG TGGTGACGCG GAAGGGTTAC CGGGTTCAGC TGCACCTGGA CTGCTGGCCC 
ACGGAGTACT ACTTCTGGCT GGAGGACGAC AGTCCCGATC TGCACCCCAT CGGCTGGTGC 
GAGGCCACCT CGCACGAACT GGAAACCCCG CCTGGCTATC TTCAGCCCAA GTCCGTGATG 
CCTTGCGATG TGGAGGGATG TCGGGGATTT GGCAACGCCA AGCGCTTCAA CCTCAACGTG 
CATGCTTTGC GGGAATGCTG TCCGTATGCG CCGGAAAATT GGCGTCAGTG GCGCTCCAAA 
ACGGTGAAAC CGCCGCGTGT GGCCCCCGAG AATATCAGAC GTGGATGGGC GAAGAAAACA 
AAGCGTGCCT GTTCGGAGGC CAAGCAAGCC ATTAAGGAGG ACTCCCAACA AGAGATTGTC 
TACCCGAAGG TCGCGGAAGT GGTGCAAGCC AAGCGAAAGA CGTCTCCTTG TGAGGAGAAG 
GTTGTGAAAA AGCAGAAACA GATGCAGAAG GAGGAGGATG CGGAGCAGGT GCCGGATGAA 
CGCTCCCTGG CCATAGCCAG GTCCTTTGTG AAGGACTATG GACCACAGTT CCTGCCAAAC 
TATCGCCTCT GGCAGCTCAA CAGCGCCTTC AAATTGGACG ACGTGCGAAC CAATCCACTC 
CACTGGACCA GCTGGGATGT GTGCGAGTAC ATAGAGCGTG CCCTGGATTC CACTGACATT 
GCCAAGGTGA TCTTCGAGCA GGACATCGAC GGCAGGGCGT TGCTGATGCT GGGGCGCAAG 
GAGTTGGATA CGTACCTGAA ACTCAAGGTG GGCCCGGCCG TGAAGCTCTA CTCCCTGATT 
CTGAATCTGC GCATCGCGGT CGTCTGCAAG TTTGATTCAA AAAACATCGA ACTGGAGAGC 
AATACTGCTG GTCCGATTCA GAGGGAGATG GATACACCTG TTTCTGCGAT AAAACAGCTG 
CCGGAGCAGT CCCAAACCAA CGGCTACAAG ACGGATCACG ACCAGGAGCT GTCCGAGAGC 
GGGGACGACG ATGACGACGA GGATGTGATG CTCGACAGCG ATGACTTTCT CAGCGTTAAG 
CCGAGCTGCC TGGTCATTGA CGAGGACGCC GATGGGGATG GCGATTGTGA ATCCGACGGC 
GATGTGGCGA TGGTGCCGAT GGAAGTGCGC ACGCCCTTGC GCACGTCCTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733209.1
CDS	788..5221
Translation	MLPLSMASAEAKQLQRNISLSMSPANGGVISPSTANCNGVASASGGNPNPNSLLTLAPRKEELRFLPLAPMGGNKTCNITISNVQPMKSKLKSVNIVPNSIKSAGAGATVGSADAPLFQLVPSPSNPSQPLVAILAPNRKTTLLGGGASNGQPVVIRSGTPLHKNSLTVETSNKPIQPGGSAAAAAVGGGTGGAPLTLVGKTVVPPKIQAATPVQIPSTGPGAQVSLLANPLKPRAPLILSKVAVDKLRLKFSQVKSNPNALVLGKANHVKKLLTSPNPSGEDKTRSTQKNNKQNTSASQLKPVVAPKTAMPIATEVPTADNNKMSLIKSKPVAIKAVPLPSQEAPIVPAVAPVVTSPEKVDVKPTAKPSIKTAPKPTPKPLEMSVNGPAKEKKAPAKEQKQKTMDKPPVVKEIAQHTPCGPKELPKLKRSKSFVSNHPASPVANNERRHSVAIMAKEVDVIEQPSAAIETITIEDDDESEEEQQKPEIKKTPKQNQKPEKQEAPKKKQIPMPILPAGITISTTSTAKKKAVRKNSSVTTISSSSSDSSSYSDVEVVPTKKTEKELSMLPGISIIKAESLPLSREDFERSLRCDEQVPQTPPKSSSSSSSGSHASEMSPPPKLADPKPSSPPAKNAKKANDHDANPSPSSKTNDFKKSMMQNLNMLKWRGQQPANLQNSTVRFELNEFNFLQINERCQPRQGPAAYFERPLYDRPGRRPSACVHPLLYLCQRCNFHGPAADFLAPHFCSVGCVRRSQKRRLPQSTQKESKISRTQLEQTAGTAPDLPQKRQTNYKPHKSLSAIQEQLQQRQKDKPRKPFRWSEYLKSKGKDVAAPIHLFLNPFPISPNCFEIGMKLEAIDPENCSLFCVCSIVEVRGYRLKLSFDGYSSMYDFWVNADSQDIFPPGWCDETARVLQAPKDYNSERFSWSRYLVKTGGKAAPRALFGHLNMQQQMDVRNGFAVGMHLEAEDLNDTGKICVATVTDILDERIRVHFDGWDDCYDLWVHITSPYIHPCGWHEGRQQLIVPPDYQKSAFIWDDYISEVGGMAASKELFTPRQPMEYQERMKLEVVDQRNPCLIRPATVVTRKGYRVQLHLDCWPTEYYFWLEDDSPDLHPIGWCEATSHELETPPGYLQPKSVMPCDVEGCRGFGNAKRFNLNVHALRECCPYAPENWRQWRSKTVKPPRVAPENIRRGWAKKTKRACSEAKQAIKEDSQQEIVYPKVAEVVQAKRKTSPCEEKVVKKQKQMQKEEDAEQVPDERSLAIARSFVKDYGPQFLPNYRLWQLNSAFKLDDVRTNPLHWTSWDVCEYIERALDSTDIAKVIFEQDIDGRALLMLGRKELDTYLKLKVGPAVKLYSLILNLRIAVVCKFDSKNIELESNTAGPIQREMDTPVSAIKQLPEQSQTNGYKTDHDQELSESGDDDDDEDVMLDSDDFLSVKPSCLVIDEDADGDGDCESDGDVAMVPMEVRTPLRTSS

Target ORF information:

RefSeq Version	NM_170330.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (3) malignant brain tumor (l(3)mbt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170330.2

ORF Insert Sequence:

ATGCTGCCAT TGTCGATGGC CAGTGCGGAG GCCAAGCAGC TCCAGCGCAA TATATCATTG 
TCCATGTCGC CAGCGAATGG AGGAGTCATC TCACCATCTA CCGCGAATTG CAATGGTGTT 
GCCTCTGCCT CCGGCGGAAA TCCAAATCCC AACAGCCTCC TCACACTAGC TCCGCGCAAG 
GAGGAGCTAC GCTTCCTGCC CCTGGCGCCC ATGGGCGGCA ACAAGACCTG CAACATCACC 
ATCAGCAATG TCCAGCCAAT GAAATCCAAA CTAAAGTCGG TCAACATAGT GCCAAATTCC 
ATAAAGTCCG CTGGAGCTGG CGCCACTGTC GGATCAGCAG ATGCTCCCCT GTTCCAGCTG 
GTGCCATCGC CCAGTAATCC CAGCCAACCG TTGGTGGCCA TTCTGGCCCC CAATCGCAAG 
ACCACGCTGC TTGGCGGAGG TGCATCCAAT GGCCAACCGG TGGTCATACG CTCCGGAACA 
CCTTTGCACA AGAACAGCCT GACCGTTGAG ACCAGCAACA AGCCCATCCA GCCAGGAGGA 
TCGGCTGCAG CAGCCGCCGT TGGAGGCGGA ACTGGAGGAG CACCTTTAAC GCTCGTGGGC 
AAAACGGTCG TTCCACCGAA AATACAAGCG GCGACACCTG TCCAGATTCC CAGCACGGGT 
CCGGGTGCGC AGGTATCCCT GCTGGCCAAT CCCCTCAAGC CACGTGCTCC ACTCATACTC 
AGCAAGGTGG CGGTGGATAA GCTGCGTCTA AAGTTCAGCC AGGTCAAGAG CAACCCAAAT 
GCACTCGTTC TCGGCAAGGC CAATCATGTG AAGAAACTTC TGACATCGCC GAACCCCAGT 
GGAGAGGATA AGACCAGGAG CACCCAGAAA AACAACAAAC AGAATACTAG TGCCAGCCAA 
CTGAAGCCAG TTGTGGCACC CAAAACGGCT ATGCCCATTG CCACGGAGGT GCCGACCGCC 
GATAACAACA AAATGAGCCT AATTAAGTCC AAGCCCGTGG CCATCAAGGC AGTGCCGCTG 
CCCAGCCAGG AAGCGCCCAT TGTGCCGGCA GTGGCGCCAG TTGTTACATC TCCAGAGAAG 
GTCGACGTTA AGCCCACTGC AAAGCCCTCA ATCAAGACAG CACCCAAACC AACTCCTAAA 
CCTCTGGAGA TGTCCGTAAA TGGACCAGCC AAGGAGAAAA AGGCGCCTGC CAAGGAGCAG 
AAACAGAAGA CAATGGACAA ACCACCGGTG GTCAAGGAAA TCGCTCAACA TACGCCATGT 
GGGCCCAAGG AATTGCCCAA ACTAAAGCGG TCGAAATCCT TCGTGTCCAA CCATCCCGCT 
TCGCCTGTGG CCAACAACGA GCGACGTCAC TCGGTGGCCA TTATGGCCAA GGAGGTGGAT 
GTGATCGAGC AGCCCAGTGC GGCAATCGAG ACCATCACCA TTGAAGACGA TGATGAGAGC 
GAGGAAGAGC AGCAGAAGCC GGAAATTAAG AAGACGCCGA AGCAGAATCA GAAGCCGGAA 
AAGCAGGAGG CGCCGAAGAA GAAGCAGATC CCCATGCCCA TTCTGCCCGC AGGAATAACC 
ATTTCCACCA CCAGTACGGC CAAGAAAAAG GCCGTGAGAA AGAACTCCTC CGTGACGACA 
ATCTCTTCTA GTTCCAGCGA CAGTTCGTCC TACAGCGATG TGGAGGTGGT ACCGACCAAG 
AAAACCGAGA AGGAACTCAG TATGCTGCCT GGCATAAGCA TCATCAAGGC GGAATCTCTG 
CCACTGAGCA GGGAGGACTT TGAACGCTCG TTGCGTTGCG ATGAGCAGGT GCCCCAAACT 
CCGCCCAAGT CATCCTCCTC ATCCTCGAGC GGCTCTCACG CATCGGAAAT GTCGCCGCCG 
CCCAAACTCG CCGATCCGAA GCCATCAAGT CCACCTGCAA AGAATGCCAA GAAAGCCAAC 
GACCATGATG CGAACCCATC TCCTAGCAGC AAGACCAATG ACTTTAAAAA GAGCATGATG 
CAGAACCTGA ACATGTTGAA ATGGCGCGGC CAGCAGCCGG CCAACCTGCA GAACTCCACC 
GTCCGGTTCG AGCTGAATGA GTTCAATTTT CTGCAGATAA ACGAACGCTG CCAGCCACGC 
CAGGGTCCAG CTGCATATTT CGAGCGGCCT TTGTATGATC GTCCGGGCCG CAGGCCCTCG 
GCCTGCGTCC ATCCGCTGCT GTACTTGTGC CAGCGTTGCA ATTTCCACGG ACCCGCCGCC 
GACTTTCTGG CACCACATTT CTGCTCCGTT GGCTGTGTGC GTCGATCCCA GAAGCGTCGT 
CTGCCGCAGA GTACGCAGAA GGAGAGCAAG ATAAGCCGTA CCCAACTAGA GCAAACAGCT 
GGAACAGCGC CAGATTTGCC ACAAAAGCGG CAAACCAACT ACAAACCACA CAAATCTCTG 
TCTGCCATAC AGGAACAGCT GCAGCAACGT CAAAAGGATA AGCCCAGAAA GCCATTCCGC 
TGGAGCGAGT ACCTCAAGTC AAAGGGAAAG GATGTGGCAG CGCCCATCCA CCTGTTCCTC 
AATCCGTTCC CCATCAGTCC CAACTGCTTC GAGATCGGCA TGAAGCTAGA GGCCATCGAT 
CCGGAGAACT GCTCGCTCTT CTGTGTCTGC AGCATTGTCG AGGTGCGCGG CTATCGGCTG 
AAGCTTAGCT TCGATGGCTA CTCCTCCATG TACGACTTCT GGGTGAACGC CGACTCGCAG 
GACATCTTTC CGCCAGGCTG GTGCGATGAG ACAGCACGCG TTCTGCAGGC GCCTAAGGAC 
TACAACTCGG AGCGCTTCAG TTGGAGCCGC TATTTGGTGA AAACGGGCGG CAAGGCAGCT 
CCGCGGGCGC TCTTTGGTCA TCTGAATATG CAGCAGCAGA TGGATGTGCG CAACGGCTTC 
GCTGTGGGCA TGCATCTCGA GGCGGAGGAC CTGAACGATA CGGGAAAGAT TTGTGTGGCC 
ACAGTGACGG ATATTCTGGA CGAGCGCATT CGAGTCCACT TCGACGGCTG GGATGACTGC 
TACGACCTGT GGGTACACAT CACCTCACCG TACATCCATC CCTGCGGTTG GCACGAGGGA 
CGCCAGCAGT TGATCGTTCC GCCGGACTAC CAGAAGTCTG CCTTCATTTG GGATGACTAC 
ATATCAGAGG TTGGTGGAAT GGCCGCCTCC AAGGAGCTGT TCACACCACG CCAGCCAATG 
GAGTACCAGG AACGCATGAA ACTGGAGGTG GTGGACCAGC GTAATCCCTG CCTAATTCGT 
CCGGCCACCG TGGTGACGCG GAAGGGTTAC CGGGTTCAGC TGCACCTGGA CTGCTGGCCC 
ACGGAGTACT ACTTCTGGCT GGAGGACGAC AGTCCCGATC TGCACCCCAT CGGCTGGTGC 
GAGGCCACCT CGCACGAACT GGAAACCCCG CCTGGCTATC TTCAGCCCAA GTCCGTGATG 
CCTTGCGATG TGGAGGGATG TCGGGGATTT GGCAACGCCA AGCGCTTCAA CCTCAACGTG 
CATGCTTTGC GGGAATGCTG TCCGTATGCG CCGGAAAATT GGCGTCAGTG GCGCTCCAAA 
ACGGTGAAAC CGCCGCGTGT GGCCCCCGAG AATATCAGAC GTGGATGGGC GAAGAAAACA 
AAGCGTGCCT GTTCGGAGGC CAAGCAAGCC ATTAAGGAGG ACTCCCAACA AGAGATTGTC 
TACCCGAAGG TCGCGGAAGT GGTGCAAGCC AAGCGAAAGA CGTCTCCTTG TGAGGAGAAG 
GTTGTGAAAA AGCAGAAACA GATGCAGAAG GAGGAGGATG CGGAGCAGGT GCCGGATGAA 
CGCTCCCTGG CCATAGCCAG GTCCTTTGTG AAGGACTATG GACCACAGTT CCTGCCAAAC 
TATCGCCTCT GGCAGCTCAA CAGCGCCTTC AAATTGGACG ACGTGCGAAC CAATCCACTC 
CACTGGACCA GCTGGGATGT GTGCGAGTAC ATAGAGCGTG CCCTGGATTC CACTGACATT 
GCCAAGGTGA TCTTCGAGCA GGACATCGAC GGCAGGGCGT TGCTGATGCT GGGGCGCAAG 
GAGTTGGATA CGTACCTGAA ACTCAAGGTG GGCCCGGCCG TGAAGCTCTA CTCCCTGATT 
CTGAATCTGC GCATCGCGGT CGTCTGCAAG TTTGATTCAA AAAACATCGA ACTGGAGAGC 
AATACTGCTG GTCCGATTCA GAGGGAGATG GATACACCTG TTTCTGCGAT AAAACAGCTG 
CCGGAGCAGT CCCAAACCAA CGGCTACAAG ACGGATCACG ACCAGGAGCT GTCCGAGAGC 
GGGGACGACG ATGACGACGA GGATGTGATG CTCGACAGCG ATGACTTTCT CAGCGTTAAG 
CCGAGCTGCC TGGTCATTGA CGAGGACGCC GATGGGGATG GCGATTGTGA ATCCGACGGC 
GATGTGGCGA TGGTGCCGAT GGAAGTGCGC ACGCCCTTGC GCACGTCCTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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l(3)mbt cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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